Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the effect of replacing corn with glycerol on ruminal bacteria in continuous culture fermenters. | the effects of substituting corn with glycerol on dna concentration of selected ruminal bacteria were investigated using continuous fermenters. four continuous culture fermenters were used in a 4 × 4 latin square design with four 10 days consecutive periods. treatment diets (60:40 forage to concentrate) were fed at 45 g/day dry matter (dm) in three equal portions. glycerol (0.995 g/g glycerol) was used to replace corn in a grain mix at proportions of 0% (t0; control), 15% (t15), 30% (t30) and 45 ... | 2010 | 20880288 |
| specified species in gingival crevicular fluid predict bacterial diversity. | analysis of gingival crevicular fluid (gcf) samples may give information of unattached (planktonic) subgingival bacteria. our study represents the first one targeting the identity of bacteria in gcf. | 2010 | 21049043 |
| comparison of the in vivo antimicrobial effectiveness of sodium hypochlorite and chlorhexidine used as root canal irrigants: a molecular microbiology study. | the purpose of this clinical study was to compare the antimicrobial effects of 2.5% sodium hypochlorite (naocl) and 0.12% chlorhexidine digluconate (chx) when used as irrigants during treatment of teeth with apical periodontitis. | 2011 | 21238793 |
| characterization of lateral flagella of selenomonas ruminantium. | selenomonas ruminantium produces a tuft of flagella near the midpoint of the cell body and swims by rotating the cell body along the cell's long axis. the flagellum is composed of a single kind of flagellin, which is heavily glycosylated. the hook length of s. ruminantium is almost double that of salmonella. | 2011 | 21335384 |
| use of 16s ribosomal rna gene analyses to characterize the bacterial signature associated with poor oral health in west virginia. | west virginia has the worst oral health in the united states, but the reasons for this are unclear. this pilot study explored the etiology of this disparity using culture-independent analyses to identify bacterial species associated with oral disease. | 2011 | 21362199 |
| cadaverine covalently linked to the peptidoglycan serves as the correct constituent for the anchoring mechanism between the outer membrane and peptidoglycan in selenomonas ruminantium. | in selenomonas ruminantium, a strictly anaerobic and gram-negative bacterium, cadaverine covalently linked to the peptidoglycan is required for the interaction between the peptidoglycan and the s-layer homologous (slh) domain of the major outer membrane protein mep45. here, using a series of diamines with a general structure of nh(3)(+)(ch(2))(n)nh(3)(+) (n = 3 to 6), we found that cadaverine (n = 5) specifically serves as the most efficient constituent of the peptidoglycan in acquiring the high ... | 2011 | 21398553 |
| clustering of subgingival microbial species in adolescents with periodontitis. | it has become increasingly recognized that groups of microorganisms interact within the subgingival plaque of adult subjects with periodontitis. it is much less clear, however, whether the consortia of microorganisms associated with periodontitis are different in early and more advanced cases of periodontitis. to investigate this point further, subgingival plaque was collected from six sites in 87 adolescents with periodontitis and 73 controls and the samples were analyzed for the detection of 1 ... | 2011 | 21410554 |
| engineering lower inhibitor affinities in β-d: -xylosidase of selenomonas ruminantium by site-directed mutagenesis of trp145. | β-d: -xylosidase/α-l: -arabinofuranosidase from selenomonas ruminantium is the most active enzyme reported for catalyzing hydrolysis of 1,4-β-d: -xylooligosaccharides to d: -xylose. one property that could use improvement is its relatively high affinities for d: -glucose and d: -xylose (k (i) ~ 10 mm), which would impede its performance as a catalyst in the saccharification of lignocellulosic biomass for the production of biofuels and other value-added products. previously, we discovered that th ... | 2011 | 21528413 |
| the effect of lipid supplements on ruminal bacteria in continuous culture fermenters varies with the fatty acid composition. | a single flow continuous culture fermenter system was used in this study to investigate the influence of dietary lipid supplements varying in their fatty acid content on the dna concentration of selected rumen bacteria. four continuous culture fermenters were used in a 4×4 latin square design with four periods of 10 d each. treatment diets were fed at 45 g/d (dm basis) in three equal portions during the day. the diets were: 1) control (con), 2) control with animal fat source (sat), 3) control wi ... | 2011 | 21538241 |
| Impact of subacute ruminal acidosis (SARA) adaptation and recovery on the density and diversity of bacteria in the rumen of dairy cows. | Subacute ruminal acidosis (SARA) is characterized by ruminal pH depression and microbial perturbation. The impact of SARA adaptation and recovery on rumen bacterial density and diversity was investigated following high-grain feeding. Four ruminally cannulated dairy cows were fed a hay diet, transitioned to a 65% grain diet for 3 weeks, and returned to the hay diet for 3 weeks. Rumen fluid, rumen solids, and feces were sampled during weeks 0 (hay), 1 and 3 (high grain), and 4 and 6 (hay). SARA wa ... | 2011 | 21692816 |
| target region selection is a critical determinant of community fingerprints generated by 16s pyrosequencing. | pyrosequencing of 16s rrna genes allows for in-depth characterization of complex microbial communities. although it is known that primer selection can influence the profile of a community generated by sequencing, the extent and severity of this bias on deep-sequencing methodologies is not well elucidated. we tested the hypothesis that the hypervariable region targeted for sequencing and primer degeneracy play important roles in influencing the composition of 16s pyrotag communities. subgingival ... | 2011 | 21738596 |
| populations of select cultured and uncultured bacteria in the rumen of sheep and the effect of diets and ruminal fractions. | the objective of this study was to assess the importance of select cultured and uncultured bacteria in the rumen by quantifying their populations and the effect of diets and ruminal fractions. full-length 16s rrna gene (rrs) sequences were recovered from rumen samples using specific primers designed from partial sequences recovered previously. five uncultured bacterial operational taxonomic units (otus) were quantified using specific quantitative pcr (qpcr) in fractionated ruminal samples from s ... | 2011 | 21822435 |
| analysis of symptomatic and asymptomatic primary root canal infections in adult norwegian patients. | this molecular study analyzed the microbiota of primary root canal infections from adult norwegian patients. | 2011 | 21846535 |
| tobacco smoking affects bacterial acquisition and colonization in oral biofilms. | recent evidence suggests that smoking affects the composition of the disease-associated subgingival biofilm, yet little is known about its effects during the formation of this biofilm. the present investigation was undertaken to examine the contributions of smoking to the composition and proinflammatory characteristics of the biofilm during de novo plaque formation. marginal and subgingival plaque and gingival crevicular fluid samples were collected from 15 current smokers and from 15 individual ... | 2011 | 21859855 |
| Evidence for the possible involvement of Selenomonas ruminantium in rumen fiber digestion. | Selenomonas ruminantium strains were isolated from sheep rumen, and their significance for fiber digestion was evaluated. Based on the phylogenetic classification, two clades of S. ruminantium (clades I and II) were proposed. Clade II is newly found, as it comprised only new isolates that were phylogenetically distant from the type strain, while all of the known isolates were grouped in the major clade I. More than half of clade I isolates displayed CMCase activity with no relation to the degree ... | 2011 | 22092507 |
| Substrate binding in protein tyrosine phosphatase-like inositol polyphosphatases. | Protein-tyrosine phosphatase-like inositol polyphosphatases are microbial enzymes that catalyze the stepwise removal of one or more phosphates from highly phosphorylated myo-inositols via a relatively ordered pathway. In order to understand the substrate specificity and kinetic mechanism of these enzymes we have determined high resolution, single crystal, X-ray crystallographic structures of inactive Selenomonas ruminantium phyA in complex with myo-inositol hexa- and penta-kisphosphate. These ... | 2011 | 22139834 |
| the subgingival microbiota of papillon-lefèvre syndrome. | background: there is little information about the microbiological profiles of periodontal lesions in papillon-lefèvre syndrome (pls) and the significance of bacteria in the pathogenesis of periodontitis in these patients. this comprehensive analysis of the subgingival microbiota in pls patients used 16s rrna clonal analysis and the 16s rrna-based human oral microbe identification microarray (homim). methods: thirteen pls patients, from 7 unrelated families, volunteered for this microbiological s ... | 2011 | 22141356 |
| Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis. | ABSTRACT: BACKGROUND: Microbial communities inhabiting human mouth are associated with oral health and disease. Previous studies have indicated the general prevalence of adult gingivitis in China to be high. The aim of this study was to characterize in depth the oral microbiota of Chinese adults with or without gingivitis, by defining the microbial phylogenetic diversity and community-structure using highly paralleled pyrosequencing. METHODS: Six non-smoking Chinese, three with and three withou ... | 2011 | 22152152 |
| Critical cellulase and hemicellulase activities for hydrolysis of ionic liquid pretreated biomass. | Critical cellulase and hemicellulase activities are identified for hydrolysis of ionic liquid (IL) pretreated poplar and switchgrass; hemicellulase rich substrates with largely amorphous cellulose. Enzymes from Aspergillus nidulans were expressed and purified: an endoglucanase (EG) a cellobiohydrolase (CBH), an endoxylanase (EX) and an acetylxylan esterase (AXE). ß-Xylosidase (ßX) from Selenomonas ruminantium and a commercial ß-glucosidase (ßG) from Novozyme 188 were admixed with the A. nidulans ... | 2012 | 22154300 |
| the correlation between composition of the bacterial community and concentration of volatile fatty acids in the rumen during the transition period and ketosis in dairy cows. | the transition period is a severe challenge to dairy cows. glucose supply cannot meet demand and body fat is mobilized, potentially leading to negative energy balance (neb), ketosis or fatty liver. propionate produces glucose by gluconeogenesis, which generation depends heavily on the number and species of microbes. in present study we analyzed the rumen microbiome composition of cows in the transition period, cows with ketosis and non-perinatal cows using terminal restriction fragment length po ... | 2012 | 22267666 |