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nitrifying microbial community analysis of nitrite accumulating biofilm reactor by fluorescence in situ hybridization.biological nitrogen removal via nitrite pathway in wastewater treatment is very important especially in the cost of aeration and as an electron donor for denitrification. wastewater nitrification and nitrite accumulations were carried out in a biofllm reactor. the biofilm reactor showed almost complete nitrification and most of the oxidized ammonium was present as nitrite at the ammonium load of 1.2 kg n/m3/d. nitrite accumulation was achieved by the selective inhibition of nitrite oxidizers by ...200312578180
spectroscopic characterization and assignment of reduction potentials in the tetraheme cytochrome c554 from nitrosomonas europaea.the tetraheme cytochrome c(554) (cyt c(554)) from nitrosomonas europaea is an essential electron transfer component in the biological oxidation of ammonia. the protein contains one 5-coordinate heme and three bis-his coordinated hemes in a 3d arrangement common to a newly characterized class of multiheme proteins. the ligand binding, electrochemical properties, and heme-heme interactions are investigated with mössbauer and x- and q-band (parallel/perpendicular mode) epr spectroscopy. the results ...200312580599
canon and anammox in a gas-lift reactor.anoxic ammonium oxidation (anammox) and completely autotrophic nitrogen removal over nitrite (canon) are new and promising microbial processes to remove ammonia from wastewaters characterized by a low content of organic materials. these two processes were investigated on their feasibility and performance in a gas-lift reactor. the anammox as well as the canon process could be maintained easily in a gas-lift reactor, and very high n-conversion rates were achieved. an n-removal rate of 8.9 kg n (m ...200312586414
use of multidimensional scaling in the selection of wastewater toxicity test battery components.in aquatic toxicity testing, no single test species is sensitive to all toxicants. therefore, test batteries consisting of several individual assays are becoming more common. the organisms in a test battery should be representative of the entire system of interest. the results of the assays should be complementary to other components in the test battery to avoid redundancy. with the aid of multidimensional scaling (mds), a multivariate statistical method, we examined the toxicity data of five bi ...200312600394
excision and integration of cassettes by an integron integrase of nitrosomonas europaea.we found in the environmental strain nitrosomonas europaea a chromosomal integron-like structure with an integrase gene, inti(neu). we have tested the capacity of the intineu integrase to excise and integrate several resistance gene cassettes. the results allow us to consider intineu a new functional integron integrase.200312618471
community structure of ammonia-oxidizing bacteria within anoxic marine sediments.the potential for oxidation of ammonia in anoxic marine sediments exists through anaerobic oxidation by nitrosomonas-like organisms, utilizing nitrogen dioxide, coupling of nitrification, manganese reduction, and anaerobic oxidation of ammonium by planctomycetes (the anammox process). here we describe the presence of microbial communities with the potential to carry out these processes in a natural marine sediment system (loch duich, scotland). natural microbial communities of planctomycetales-v ...200312620817
biological treatment process of air loaded with an ammonia and hydrogen sulfide mixture.the physico-chemical characteristics of granulated sludge lead us to develop its use as a packing material in air biofiltration. then, the aim of this study is to investigate the potential of unit systems packed with this support in terms of ammonia and hydrogen sulfide emissions treatment. two laboratory scale pilot biofilters were used. a volumetric load of 680 g h2s m(-3) empty bed day(-1) and 85 g nh3 m(-3) empty bed day(-1) was applied for eight weeks to a unit called bgsn (column packed wi ...200312656240
nitrogen isotopomer site preference of n2o produced by nitrosomonas europaea and methylococcus capsulatus bath.the relative importance of individual microbial pathways in nitrous oxide (n(2)o) production is not well known. the intramolecular distribution of (15)n in n(2)o provides a basis for distinguishing biological pathways. concentrated cell suspensions of methylococcus capsulatus bath and nitrosomonas europaea were used to investigate the site preference of n(2)o by microbial processes during nitrification. the average site preference of n(2)o formed during hydroxylamine oxidation by m. capsulatus b ...200312661029
activity and population dynamics of heterotrophic and ammonia-oxidizing microorganisms in soil surrounding sludge bands spiked with linear alkylbenzene sulfonate: a field study.recent research has documented soil microorganisms to be rather sensitive to linear alkylbenzene sulfonates (las), which may enter the soil environment in considerable quantities following sewage sludge disposal. we here report field effects of las on selected microbial populations present in a sandy soil surrounding well-defined sludge bands spiked with high but realistic las levels (7.1 or 31.3 g/kg). surprisingly, las had no effect on heterotrophic respiration in the sludge compartment per se ...200312685718
selective one-electron reduction of nitrosomonas europaea hydroxylamine oxidoreductase with nitric oxide.hydroxylamine oxidoreductase (hao) from the autotrophic bacterium nitrosomonas europaea catalyzes the 4-e- oxidation of nh2-oh to no2-. the e- are transferred from nh2oh to an unusual 5-coordinate heme known as p460, which is the active site of hao, and from there to an array of seven c-type hemes. no., generated by laser flash photolysis of n,n'-bis(carboxymethyl)-n,n'-dinitroso-1,4-phenylenediamine, is found to act as a 1-e- donor to hao. most likely no. binds p460 to yield a [fe(no)]6 moiety, ...200312693206
lessons from the genome of a lithoautotroph: making biomass from almost nothing. 200312700247
complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph nitrosomonas europaea.nitrosomonas europaea (atcc 19718) is a gram-negative obligate chemolithoautotroph that can derive all its energy and reductant for growth from the oxidation of ammonia to nitrite. nitrosomonas europaea participates in the biogeochemical n cycle in the process of nitrification. its genome consists of a single circular chromosome of 2,812,094 bp. the gc skew analysis indicates that the genome is divided into two unequal replichores. genes are distributed evenly around the genome, with approximate ...200312700255
microbial composition and structure of a rotating biological contactor biofilm treating ammonium-rich wastewater without organic carbon.high nitrogen losses were observed in a rotating biological contactor (rbc) treating ammonium-rich (up to 500 mg nh4(+)-n/l) but organic-carbon-poor leachate from a hazardous waste landfill in kölliken, switzerland. the composition and spatial structure of the microbial community in the biofilm on the rbc was analyzed with specific attention for the presence of aerobic ammonium and nitrite oxidizing bacteria and anaerobic ammonium oxidizers. anaerobic ammonium oxidation (anammox) involves the ox ...200312704553
cytochrome p460 of nitrosomonas europaea. formation of the heme-lysine cross-link in a heterologous host and mutagenic conversion to a non-cross-linked cytochrome c'.the heme of cytochrome p460 of nitrosomonas europaea, which is covalently crosslinked to two cysteines of the polypeptide as with all c-type cytochromes, has an additional novel covalent crosslink to lysine 70 of the polypeptide [arciero, d.m. & hooper, a.b. (1997) febs lett.410, 457-460]. the protein can catalyze the oxidation of hydroxylamine. the gene for this protein, cyp, was expressed in pseudomonas aeruginosa strain pao laci, resulting in formation of a holo-cytochrome p460 which closely ...200312709052
susceptibility of ammonia-oxidizing bacteria to nitrification inhibitors.activity of nitrification inhibitors to several typical ammonia-oxidizing bacteria isolated recently, i. e. nitrosococcus, nitrosolobus, nitrosomonas, nitrosospira and nitrosovibrio species was assayed using 2-amino-4-methyl-trichloromethyl-1,3,5-triazine (mast), 2-amino-4-tribromomethyl-6-trichloromethyl-1,3,5-triazine (br-mast), 2-chloro-6-trichloromethylpyridine (nitrapyrin) and others, and compared to confirm the adequate control of ammonia-oxidizing bacteria by the inhibitors. the order of ...200312710742
cooxidation of naphthalene and other polycyclic aromatic hydrocarbons by the nitrifying bacterium, nitrosomonas europaea.the soil nitrifying bacterium nitrosomonas europaea has shown the ability to transform cometabolically naphthalene as well as other 2- and 3-ringed polycyclic aromatic hydrocarbons (pahs) to more oxidized products. all of the observed enzymatic reactions were inhibited by acetylene, a selective inhibitor of ammonia monooxygenase (amo). a strong inhibitory effect of naphthalene on ammonia oxidation by n. europaea was observed. naphthalene was readily oxidized by n. europaea and 2-naphthol was det ...200212713129
structure and activity of multiple nitrifying bacterial populations co-existing in a biofilm.a biofilm from a nitrifying pilot-scale sequencing batch reactor was investigated for effects of varying process conditions on its microscale activity and structure. microsensor measurements of oxygen, substrates and products of nitrification were applied under incubation at different ammonium and oxygen concentrations which reflected various situations during a treatment cycle. a high net n loss was observed under high ammonium (ha) concentrations in contrast to low ones. additionally, results ...200312713462
nitrification efficiency and nitrifying bacteria abundance in combined as-rbc and a2o systems.this study makes a comparison between the nitrification performance of tncu-i (a combined activated sludge-rotating biological contactor process) and a2o systems by the use of a pilot plant and batch experiments. the nitrifier abundance in both systems was determined, using cloning-denaturing gradient gel electrophoresis (dgge) and fluorescent in-situ hybridization (fish), to investigate the role of rotating biological contactor in the tncu-i process. the stability of the nitrification performan ...200312727236
optimization of single-base-pair mismatch discrimination in oligonucleotide microarrays.the discrimination between perfect-match and single-base-pair-mismatched nucleic acid duplexes was investigated by using oligonucleotide dna microarrays and nonequilibrium dissociation rates (melting profiles). dna and rna versions of two synthetic targets corresponding to the 16s rrna sequences of staphylococcus epidermidis (38 nucleotides) and nitrosomonas eutropha (39 nucleotides) were hybridized to perfect-match probes (18-mer and 19-mer) and to a set of probes having all possible single-bas ...200312732557
community analysis of ammonia and nitrite oxidizers during start-up of nitritation reactors.partial nitrification of ammonium to nitrite under oxic conditions (nitritation) is a critical process for the effective use of alternative nitrogen removal technologies from wastewater. here we investigated the conditions which promote establishment of a suitable microbial community for performing nitritation when starting from regular sewage sludge. reactors were operated in duplicate under different conditions (ph, temperature, and dilution rate) and were fed with 50 mm ammonium either as syn ...200312788718
characterization of an autotrophic nitrogen-removing biofilm from a highly loaded lab-scale rotating biological contactor.in this study, a lab-scale rotating biological contactor (rbc) treating a synthetic nh(4)(+) wastewater devoid of organic carbon and showing high n losses was examined for several important physiological and microbial characteristics. the rbc biofilm removed 89% +/- 5% of the influent n at the highest surface load of approximately 8.3 g of n m(-2) day(-1), with n(2) as the main end product. in batch tests, the rbc biomass showed good aerobic and anoxic ammonium oxidation (147.8 +/- 7.6 and 76.5 ...200312788771
factors influencing spatial variability in nitrogen processing in nitrogen-saturated soils.nitrogen (n) saturation is an environmental concern for forests in the eastern u.s. although several watersheds of the fernow experimental forest (fef), west virginia exhibit symptoms of n saturation, many watersheds display a high degree of spatial variability in soil n processing. this study examined the effects of temperature on net n mineralization and nitrification in n-saturated soils from fef, and how these effects varied between high n-processing vs. low n-processing soils collected from ...200112805879
linking nitrifying biofilm structure and function through fluorescent in situ hybridization and evaluation of nitrification capacity.fluorescent in situ hybridization (fish) was used to quantify the ammonia-oxidizing populations within intact biofilm samples collected from a full-scale nitrifying trickling filter (ntf). ammonia, nitrite, and nitrate concentrations were measured for aqueous samples taken in conjunction with biofilm samples at multiple filter depths. the ammonia removal capability of collected biofilms was evaluated by transferring the biofilms to laboratory batch reactors. nitrate plus nitrite generation rates ...200312837026
molecular biological methods (dgge) as a tool to investigate nitrification inhibition in wastewater treatment.incomplete nitrification at an activated sludge plant for biological pre-treatment of rendering plant effluents led to a detailed investigation on the origin and solution of this problem. preliminary studies revealed that an inhibition of ammonia oxidising microorganisms (aom) by process waters of the rendering plant was responsible for the situation. we were able to show a correlation between the existence of specific aom and nitrification capacity expressed as oxygen uptake rate for maximal ni ...200312906286
characterization of microbial communities and composition in constructed dairy wetland wastewater effluent.constructed wetlands have been recognized as a removal treatment option for high concentrations of contaminants in agricultural waste before land application. the goal of this study was to characterize microbial composition in two constructed wetlands designed to remove contaminants from dairy washwater. water samples were collected weekly for 11 months from two wetlands to determine the efficiency of the treatment system in removal of chemical contaminants and total and fecal coliforms. the red ...200312957887
wide distribution of a novel pmoa-like gene copy among type ii methanotrophs, and its expression in methylocystis strain sc2.experiments were conducted to determine if a novel pmoa-like gene (pmoa2) recently discovered in the methane-oxidizing bacterium methylocystis strain sc2 (p. f. dunfield, m. tchawa yimga, s. d. dedysh, u. berger, w. liesack, and j. heyer, fems microbiol. ecol. 41:17-26, 2002) is present in other methane-oxidizing bacteria (mob), and if it is expressed. a newly developed primer combination (pmoa206f-pmoa703b) allowed a differential detection of pmoa1 and pmoa2. by using this primer combination, w ...200312957949
[pure cultures of nitrosomonas]. 195212998527
the biochemistry of the nitrifying organisms. i. the ammonia oxidizing systems of nitrosomonas. 195213018177
the biochemistry of the nitrifying organisms. ii. the free-energy efficiency of nitrosomonas. 195213018178
the respiration of nitrosomonas. 195213018311
the intermediary metabolism of nitrosomonas. 195213018312
iron and the nitrifying bacteria. 195313035032
composition and cytology of nitrosomonas. 195313037653
the biochemistry of the nitrifying organisms. iii. composition of nitrosomonas. 195313058873
the biochemistry of the nitrifying organisms. iv. the respiration and intermediary metabolism of nitrosomonas. 195313058954
[development of heterotrophes in inorganic medium and role of ammonium]. 195313095323
[feeding of nitrosomonas europea on organic substances]. 195313116876
[oxidation of ammonia and synthesis of protein in pure cultures of nitrosomonas europea]. 195313116891
laboratory studies on the kinetics of the growth of nitrosomonas with relation to the nitrification phase of the b.o.d. test. 195413125339
16s rrna and amoa-based phylogeny of 12 novel betaproteobacterial ammonia-oxidizing isolates: extension of the dataset and proposal of a new lineage within the nitrosomonads.the phylogenetic relationship of 12 ammonia-oxidizing isolates (eight nitrosospiras and four nitrosomonads), for which no gene sequence information was available previously, was investigated based on their genes encoding 16s rrna and the active site subunit of ammonia monooxygenase (amoa). almost full-length 16s rrna gene sequences were determined for the 12 isolates. in addition, 16s rrna gene sequences of 15 ammonia-oxidizing bacteria (aob) published previously were completed to allow for a mo ...200313130037
[obtaining pure cultures of nitrosomonas]. 195313144375
[chemistry of nitrification]. 195413151064
[isolation of pure cultures of nitrosomonas europae]. 195413173366
[acellular nitrification. i. growth of cultures of nitrosomas and formation of cell autolysates]. 195413203080
[acellular nitrification. ii. oxidation of ammonia with autolysates of nitrosomonas]. 195413223516
[acellular nitrifikation. iii. dynamics of concentration of nitrites]. 195513296884
[acellular nitrification. iv. heat inactivation of ferments of nitrosomonas europea oxidizing ammonia]. 195613321524
[relation of nitrosomonas to organic substances]. 195613321539
estimation of numbers of nitrosomonas in soil and culture. 195613321980
[acellular nitrification. v. oxidation of hydroxylamine in acellular autolysates of nitrosomnas]. 195613369172
[possibility of heterotrophic metabolism in nitrifying bacteria]. 195613407450
preservation of nitrosomonas. 195713418790
preservation of nitrosomonas. 195713440942
[accumulation of organic substances by nitrosomonas europaea cultures grown on winogradsky's medium]. 195713477106
[relation of nitrosomonas to radioactive saccharose and carbonate]. 195713483120
growth and autotrophic metabolism of nitrosomonas europaea. 195813563419
influence of corn steep liquor on the oxidation of ammonia to nitrate by nitrosomonas europaea. 195813575767
[the behavior of nitrosomonas europaea after dessication]. 195813589713
isolation of nitrosomonas in pure culture. 195813598713
[nitrogen metabolism in nitrosomonas europaea]. 195813622242
morphology of nitrosomonas europaea and classification of the nitrifying bacteria. 196113696807
[is nitrosomonas capable of assimilating organic substances from the air?]. 196013726365
[the pigmented form of nitrosomonas europaea]. 196013763903
[adaptation of nitrosomonas to conditions of various natural substrates]. 196113779063
[isolation of pure cultures of nitrosomonas from various natural substrates and their characteristics]. 196013779064
[nitrifying activity of pure and accumulated nitrosomonas cultures isolated from various natural substrates]. 196113779065
[on the ecology of nitrosomonas]. 196113779066
enzymatic activity of nitrosomonas extracts. 195913820316
nitrohydroxyl-amine: the unknown intermediate in nitrification? 196213860225
[the effect of light on nitrosomonas europaea win]. 196213909041
[adaptation of nitrosomonas ectotypes to concentrations of organic substances in culture media]. 196213923650
[the physiological role of cytochrome in nitrifying bacteria]. 196213931334
use of electron paramagnetic resonance spectroscopy in investigations of functional metal components in micro-organisms. 196213938326
autotrophic enzyme systems. i. electron transport systems concerned with hydroxylamine oxidation in nitrosomonas. 196314011836
phosphorylation by extracts of nitrosomonas europaea.burge, w. d. (university of california, berkeley), e. malavolta, and c. c. delwiche. phosphorylation by extracts of nitrosomonas europaea. j. bacteriol. 85:106-110. 1963.-cellfree preparations of nitrosomonas europaea are capable of oxidizing hydroxylamine, but not ammonium ion, to nitrite. the quantity of nitrite formed by our preparations was, at most, equivalent to only 70% of the hydroxylamine added. although the preparations had a strong phosphatase activity, resulting in a net loss of orga ...196314016952
[bacterial associations and biological alterations of limestone monuments]. 196314048726
adenosine triphosphate-dependent reduction of nicotinamide adenine dinucleotide by ferro-cytochrome c in chemoautotrophic bacteria. 196314087009
some properties of a hydroxylamine oxidase from nitrosomonas europaea. 196314090439
some organic transformation effected by cell-free preparations of nitrosomonas. 196314099563
the incorporation of labelled co2 into cells and extracts of nitrosomonas europaea. 196414123573
occurrence of cytochrome oxidase and peroxidase in nitrosomonas europaea. 196414165498
the synthesis of atp and the incorporation of 32p by cell-free preparations from nitrosomonas europaea. 196414192897
biochemistry of the cell wall of nitrosomonas europaea. 196414200691
[the behavior of nitrifying bacteria towards dissolved oxygen]. 196414245150
hydroxylamine formation by nitrosomonas europaea. 196414272491
determination of kinetic constants for nitrifying bacteria in mixed culture, with the aid of an electronic computer. 196514287204
studies on the formation of nitrogenous gas from hydroxylamine by nitrosomonas. 196514292843
structure of nitrosocystis oceanus and comparison with nitrosomonas and nitrobacter.murray, r. g. e. (university of western ontario, london, ont., canada), and s. w. watson. structure of nitrosocystis oceanus and comparison with nitrosomonas and nitrobacter. j. bacteriol. 89:1594-1609. 1965.-nitrosocystis oceanus has distinctive features: the cell wall (overall thickness, 250 a) has an inner triplet structure and a dense enveloping layer; between these lie the "cell-wall organelles" (two or more per cell; plaques about 0.5 mu in diameter and 0.1 mu thick) of unknown function an ...196514293026
the effect of 2-chloro, 6-(trichloromethyl) pyridine on the chemoautotrophic metabolism of nitrifying bacteria. i. ammonia and hydroxylamine oxidation by nitrosomonas. 196514315631
[studies on the influence of temperature on nitrification in the elbe estuary]. 196414320216
continuous flow method in soil microbiology. v. nitrification. 196514332135
estimation of the nitric oxide formed from hydroxylamine by nitrosomonas.1. nitric oxide that was produced by reducing nitrite with an excess of acidified potassium iodide under nitrogen in warburg respirometer flasks was rapidly absorbed by a solution of permanganate in sodium hydroxide held in the side arm. a small amount of nitrous oxide (or nitrogen) that was also produced was not absorbed. 2. by using a quantitative method for the recovery of nitrite from samples of the alkaline permanganate, it was found that the sum of the nitrite n formed and the residual nit ...196514342235
studies on the oxidation of ammonia by nitrosomonas.1. free-energy calculations for ph7 showed that the oxidation of ammonia to hydroxylamine is endergonic and that the oxidations of hydroxylamine to nitrite and hydrazine to nitrogen are exergonic. it is suggested that the oxidation of ammonia requires the expenditure of energy. 2. the anaerobic dehydrogenation of hydrazine to nitrogen by extracts of the autotrophic nitrifying micro-organism, nitrosomonas, in the presence of methylene blue as electron acceptor, was less rapid than the anaerobic d ...196514342504
[comparative studies on the effect of visible light on nitrosomonas europaea and nitrobacter winogradskyi]. 196514347921
[myxobacillus accepted as nitrifying bacteria]. 195514383367
[verification of purity of nitrosomonas cultures]. 195514383368
[relation of nh4 ion oxidation processes by nitrite bacteria to certain environmental factors. ii. effect of temperature]. 195914418614
[relation of the process of oxidation of ammonium ion by nitrite bacteria to certain factors of the medium. i. effect of concentrations of ammonium sulfate]. 195914418616
[morphology of nitrosomonas europaea]. 196014439829
growth rates of nitrifying chemoautotrophs in soil. 196214476222
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