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effect of indomethacin on egg transport and pregnancy in the rabbit.treatment of rabbits with indomethacin (10 mg/kg/day) 48 hr before mating, and with 20 mg/kg at 12 hr followed by 8 mg/kg at 48, 72 or 96 hr after mating did not affect the rate of egg transport through the oviduct. indomethacin treatment at the time of implantation interfered with pregnancy and caused degeneration and resorption of embryos. these results suggest that inhibition of prostaglandin synthesis does not directly affect egg transport, but that prostaglandin appears to be required for t ...19761255565
fertilizing ability in vivo and in vitro of spermatozoa of rats and mice treated with alpha-chlorohydrin.the fertilizing ability of epididymal spermatozoa from rats and mice treated for 3 or 4 or 9 or 10 days with various doses of alpha-chlorohydrin was tested in vitro, and in vivo by intrauterine insemination. the minimum doses (per kg/day) needed to affect fertilization significantly were: rat, in vitro, 8-8 mg for 3 or 4 days, 4-4 mg for 4 days and 2-7 mg for 9 or 10 days; in vivo, 4-4 mg for 3 or 4 days and 2-7 mg for 9 or 10 days: mouse, in vitro, 4-4 mg for 3 days and 13-3 mg for 9 days; in v ...19761255566
proceedings: morphological and functional changes in the corpus luteum after restriction of ovarian blood flow. 19761255622
proceedings:steroid and prostaglandin f concentration in human corpora lutea in relation to luteolysis. 19761255623
proceedings: uptake and incorporation of (u-14c)glucose during culture of mouse blastocysts previously undergoing normal implantation and induced delay of implantation. 19761255628
proceedings: proteins of the uterine secretion in sheep. 19761255629
leukocyte alkaline phosphate determinations and urinary estriol excretion in normal and pathologic pregnancies.a comparison of the results of two assays for monitoring fetal well-being in normal and pathologic pregnancies is described. concomitant leukocyte alkaline phosphatase (lap) score determinations and 24-hour urinary estriol excretion were preformed in the same groups of pregnant women from the 31st week of pregnancy onward. there were 45 women with normal pregnancies, 10 women with antepartum death, 8 women in whom the membranes ruptured at least 30 hours before the determination, 40 women with m ...19901255642
the pill and the breast.estrogen and progesterone, although of a synthetic type in contraceptive pills, play a major role in the development, growth and function of the breasts. there is no statistically valid evidence to indicate that the widespread use of estrogens for contraceptive purposes and for the menopausal syndrome has increased the incidence of benign, premalignant or malignant breast lesions. however, if a cancer is present in a woman's breast, estrogen can increase its rate of growth. furthermore, while th ...19901255643
etodolic acid and related compounds. chemistry and antiinflammatory actions of some potent di- and trisubstituted 1, 3, 4, 9-tetrahydropyrano[3, 4-b]indole-1-acetic acids.a series of 37 1-ethyl- and 1-n-propyl-1, 3, 4, 9-tetrahydropyrano[3, 4-b]indole-1-acetic acids bearing one, or two, substituents on the benzene ring has been synthesized via the acid-catalyzed condensation of a substituted tryptophol with ethyl propionylacetate or ethyl butyrylacetate. antiinflammatory and ulcerogenic effects were examined and the results show that 1, 8-diethyl-1, 3, 4, 9-tetrahydropyrano[3, 4-b]indole-1-acetic acid (etodolic acid, usan) is a potent agent, particularly active a ...19761255663
the lehigh--medical college of pennsylvania six-year b.a.-m.d. program.lehigh university and the medical college of pennsylvania have developed a program in which selected high school students are admitted directly to medical school. the students spend two years at the university completing the premedical basic sciences and the required humanities and social sciences for the bachelor's degree. students may choose to extend the two-year university phase to three or four years. science credit is given by the university for medical school courses, and the student grad ...19901255688
the medroxyprogesterone acetate intravaginal silastic ring as a contraceptive device. 20051261263
evaluation of the surgical clerkship experience in affiliated hospitals: performance on objective examinations. 19911263481
the treatment of eating disorders. 19931263597
a feedback system for evaluating educational programmes. 19931263617
modeling health services systems.a conceptual model for the analysis of personal and collective health services systems is proposed. by placing existing formal models in the framework of this conceptual model, we emphasized their qualities and shortcomings. we discussed the normative and descriptive approach of formal modeling. finally we emphasized the close conceptual parallel that exists between personal and collective health services systems.19931263622
mailed versus telephoned appointment reminders to reduce broken appointments in a hospital outpatient department.this study compared mail, telephone, and control strategies to reduce the rate of broken appointments in a pediatric outpatient department. based on 1,039 randomly assigned appointments, the mail strategy had a broken appointment rate of 29.1 per cent, the telephone strategy of 25.3 per cent, and the control of 44.2 per cent. both strategies were significantly effective, but the difference between them was not significant. patients without telephones had a higher rate of broken appointments than ...19931263624
[available list of adverse effects]. 19941263655
[better education--better ambulances]. 19941263670
[internship or residency]. 19951263782
[letter: monoamine oxidase inhibitors]. 19961263804
[test of degranulation of basophils and mast cells for detection of allergic diseases (literature survey)]. 19761274295
letter: eggs and hypercholesterolaemia. 20051276801
eae: a model for immune intervention with synthetic peptides.the cellular and molecular requirements for the autoimmune disease eae are being defined in increasing detail through intense scrutiny of critical autoantigenic peptides, class ii mhc molecules, and alpha beta tcrs involved in the disease process. this study has led to novel immunotherapeutic approaches, many of which are based on the administration of synthetic peptides. since short peptides are understood to be the minimal antigenic units bound by mhc molecules for recognition by t cells, they ...19921285062
cns glial scar tissue: a source of molecules which inhibit central neurite outgrowth. 19921287723
pneumonia associated with salmonella choleraesuis infection in swine: 99 cases (1987-1990).salmonella choleraesuis was isolated in pure or mixed bacterial cultures from 153 swine necropsied between jan 1, 1987 and dec 31, 1990. pneumonia was seen in 99 of 109 swine from which this bacterium was isolated in the absence of other pathogenic bacteria. pneumonia was seen more frequently than hepatitis, splenomegaly, or colitis. pleuropneumonia that was grossly indistinguishable from the pleuropneumonia associated with actinobacillus pleuropneumoniae was seen in 29 of 99 swine from which s ...19921289347
feeling good affects your work. a team approach to health and productivity. 20051291285
serum haptoglobin concentration in swine naturally or experimentally infected with actinobacillus pleuropneumoniae.serum haptoglobin (hp) concentrations were measured in swine that were naturally or experimentally infected with actinobacillus pleuropneumoniae. in swine from a specific-pathogen-free herd, mean serum concentration of hp (+/- sd) was 5.79 +/- 1.06 mg of cyanmethemoglobin-binding capacity (chbc)/dl. serum hp concentrations in paired samples were measured at 7-day intervals in 40 swine randomly selected from a conventional herd that was experiencing an acute episode of pneumonia and deaths caused ...19921293115
adjuvant properties of propionibacterium avidum kp-40 in vaccination against endemic viral and bacterial infections. ii. swine immunized with inactivated haemophilus pleuropneumoniae vaccine and experimentally infected with different virulent serotypes of h. pleuropneumoniae.forty 3-month old swine were treated with immunomodulating propionibacterium avidum kp-40 (pa) and/or vaccinated with a formalin-inactivated mixture of serotypes 1, 3, 5 and 9 of haemophilus pleuropneumoniae (pleurovac). three weeks after revaccination all animals were inoculated with viable single serotypes of haemophilus pleuropneumoniae. the igg antibodies induced by vaccination agglutinated all serotypes of haemophilus pleuropneumoniae, except for serotype 5. antibody titers were not influen ...19921303696
demonstration of atrioventricular dissociation due to complete heart block by pulsed doppler echocardiography of hepatic veins. 20051307094
synthesis and structure-activity relationships of 7-diazabicycloalkylquinolones, including danofloxacin, a new quinolone antibacterial agent for veterinary medicine.a series of novel 6-fluoro-7-diazabicycloalkylquinolonecarboxylic acids substituted with various c8 (h, f, cl, n) and n1 (ethyl, cyclopropyl, vinyl, 2-fluoroethyl, 4-fluorophenyl, 2,4-difluorophenyl) substituents, as well as, 9-fluoro-10-diazabicycloalkylpyridobenzoxazinecarboxylic acids, were prepared and evaluated for antibacterial activity against a range of important veterinary pathogenic bacteria. the diazabicycloalkyl side chains investigated at the 7-position (benzoxazine 10-position) inc ...19921311762
association of the rtx proteins of actinobacillus pleuropneumoniae with hemolytic, camp, and neutrophil-cytotoxic activities.the immunoglobulin g from a monospecific rabbit antiserum to the 110-kda rtx hemolysin of actinobacillus pleuropneumoniae serotype 1 was used to determine that the related rtx proteins in isolates from serotypes 2 to 12 were also responsible for the hemolytic, camp, and neutrophil-cytotoxic activities produced by this bacterium. these proteins share common neutralizing epitopes.19921314227
variation in seropositivity for some respiratory disease agents in finishing pigs: epidemiological studies on some health parameters and farm and management conditions in the herds.the relationship between the extent of seropositivity for aujeszky's disease virus (adv), actinobacillus pleuropneumoniae (app.) serotype 2 and porcine influenza (pi) viruses serotype h1n1 and h3n2 in pigs on the one hand and the health status of the pigs and some farm and management conditions in the herds on the other hand was studied in 45 pig finishing herds, all members of one integration group. the health status was assessed by the extent of clinical signs, the use of veterinary drugs and ...19921315466
the pathogenicity of drug-resistant variants of herpes simplex virus. 19921317602
is there a place for phil donahue in the classroom?the novel approach of using the phil donahue television talk show model in the classroom was successful for us. the model worked well with a panel of guests providing content and the faculty member moderating and directing the discussion by targeting questions and limiting and/or expanding responses as dictated by the objectives for the class. we hypothesize that the johnny carson format, where a single guest speaker is interviewed by a faculty member, also would produce excellent results. we en ...19811320112
adult-onset metachromatic leukodystrophy presenting as isolated peripheral neuropathy.a 38-year-old man presented with weakness of the lower limbs. electrophysiology revealed a pronounced demyelinating neuropathy. nerve biopsy disclosed de- and remyelinating lesions and characteristic lamellar inclusions in schwann cells and macrophages. there was no familial history of neurologic disorder, and impairment of motor evoked potentials was the only sign of cns involvement. arylsulfatase a and cerebroside sulfate sulfatase activities in leukocytes and cultures of the patient's fibrobl ...19921320219
cholecystokinin, gastrin and stress hormone responses in marathon runners.the purpose of this investigation was to determine the influence of long-distance running on the secretion of the gastrointestinal peptide hormones cholecystokinin (cck) and gastrin. several known stress hormones, acth, cortisol and norepinephrine, were also measured. the hormones were estimated before and after a competitive marathon run of 46.5 km and under control conditions a few weeks later. except gastrin, all hormones were significantly higher under prerun conditions than under control co ...19821320260
monitoring of the neuromuscular transmission by electromyography (i). stability and temperature dependence of evoked emg response compared to mechanical twitch recordings in the cat.the stability over time and the effect of muscle temperature change were evaluated for the evoked compound emg and for the mechanomyogram of the tibialis anterior muscle of 7 anaesthetized cats. both emg areas and amplitudes were recorded. during stimulation for 3 h with 0.1 hz (one leg) and train-of-four (tof) (the other leg), the emg was stable while the mechanomyogram initially increased 35-50% in the first 7-8 min and then decreased 19-22% and 5-8% over the first and second 1.5-h period, res ...19921325093
clinical observation and neurological outcomes in "alice in wonderland" syndrome.ten patients, aged from 3 to 10 years, who had either had attacks of perception errors of body schema and objects or had visual hallucination were enrolled from 1987 to 1990. seven were boys and three, girls. the metamorphopsia or visual hallucination that has been named the "alice in wonderland syndrome" (aiws) were the leading presentations. all the patients had preceding episodes of upper respiratory tract infection (uri) from two days to four weeks previously except for one who had had chick ...19921325095
comparison of the cytolysin ii genetic determinants of actinobacillus pleuropneumoniae serotypes.cytolysins (cly) i, ii, and iii are toxins secreted by actinobacillus pleuropneumoniae. these toxins are thought to play an important role in the pathogenesis of porcine pleuropneumonia. clyi and clyii are rtx toxins and in general these toxins are encoded by operons consisting of four genes, c, a, b, and d. our group recently cloned the c and a genes of the clyii operon (clyiica) of serotype 9. we found that this clyii operon is truncated and lacked intact b and d genes (clyiibd). b and d genes ...19921339412
purification of hlyx, a potential regulator of haemolysin synthesis, and properties of hlyx:fnr hybrids.the hlyx gene of the swine pathogen actinobacillus pleuropneumoniae is homologous to fnr, an anaerobic transcriptional regulator of escherichia coli. it endows a haemolytic phenotype upon e. coli, and will complement the anaerobic respiratory deficiencies of fnr mutants of e. coli. the coding region of the hlyx gene was expressed in e. coli and the hlyx protein was purified by using an assay based on its immunological cross-reactivity with anti-fnr antibodies. the hlyx protein had the predicted ...19921355913
effects of mutations altering sos regulation on a nalidixic acid-inducible system for the production of heterologous proteins in escherichia coli.the major leftward early promoter of phage lambda pl, has frequently been used to drive expression of heterologous genes in escherichia coli. pl is typically maintained fully repressed by the lambda cl protein. when induction of heterologous protein synthesis is desired, one of several potential mechanisms of destroying cl function is employed and the expression of the foreign gene commences. one method of derepressing pl involves exposing cells to nalidixic acid, which results in the "activatio ...19901366870
biochemical characterization of an antigenic saline extract of actinobacillus pleuropneumoniae serotype 5 and identification of a serotype-specific antigen for elisa serodiagnosis.a saline extract of boiled-formalinized whole cells from a local strain (81-750; quebec, canada) of actinobacillus pleuropneumoniae, serotype 5b was used as an antigen in an enzyme-linked immunosorbent assay (elisa) for serodiagnosis of swine pleuropneumonia. characterization of this crude extract was done and proteins, neutral sugars, hexosamines, and 2-keto-3-deoxyoctonate (kdo) were evaluated. on phenol extraction of the crude extract a serotype-specific antigen of polysaccharidic nature was ...19921374980
serological heterogeneity of actinobacillus (haemophilus) pleuropneumoniae serotype 5 strains. 19921376626
characterization of the lipopolysaccharide o antigens of actinobacillus pleuropneumoniae serotypes 9 and 11: antigenic relationships among serotypes 9, 11, and 1.the antigenic lipopolysaccharide o polysaccharides of capsular serotypes 9 and 11 were examined by chemical, immunological, and nuclear magnetic resonance methods. immunodiffusion tests carried out on these o antigens indicated that both contained common epitopes which were also shared by actinobacillus pleuropneumoniae serotype 1. chemical analysis and high-field nuclear magnetic resonance spectroscopy showed that the o antigens of serotypes 9 and 11 were high-molecular-weight polymers consisti ...19921379584
inhibition of bactericidal activity of anticapsular antibody by nonspecific antibodies reactive with surface-exposed antigenic determinants on actinobacillus pleuropneumoniae.in an attempt to understand the mechanism of serum resistance in actinobacillus pleuropneumoniae, in the present study we examined various interactions among the bacterial surface constituents, serum antibodies, and complement. analysis of swine sera revealed the presence of anticapsular antibodies in convalescent-phase sera but not in preimmune sera. both types of sera contained antibodies which reacted with each of 14 polypeptides present in saline extracts of the bacteria. absorption of the p ...19921379990
haematological and clinicochemical blood profiles in slaughter pigs.in a cohort study, 40 pig finishing herds were selected: twenty pig herds with a low and twenty pig herds with a high prevalence of several pathological lesions recorded at slaughter in a six-month period before the start of the study. blood samples were taken from 20 pigs per herd at the end of the finishing period to investigate haematological and clinicochemical profiles. there was only a significant difference in serum albumin concentration between the low and high lesion prevalence groups. ...19921380195
identification of the cross-reacting antigen among actinobacillus pleuropneumoniae strains of serotype 1, 9 and 11 by use of monoclonal antibodies.two monoclonal antibodies (mabs), lmab-1 and lmab-5, against actinobacillus pleuropneumoniae serotype 1 were obtained. in enzyme-linked immunosorbent assay-inhibition tests with whole cell antigens obtained from serotype 1 to 12 strains of a. pleuropneumoniae, lmab-1 reacted to only a serotype 1, strain 4074. the epitope recognized by lmab-1 was a carbohydrate sensitive to periodate oxidation and resided on capsular polysaccharide (cp) of a. pleuropneumoniae serotype 1. on the other hand, lmab-5 ...19921382625
characterization of monoclonal antibodies against actinobacillus pleuropneumoniae serotype 5.two monoclonal antibodies against actinobacillus pleuropneumoniae serotype 5, designated as 5mab-1 and 5mab-6, were characterized. enzyme-linked immunosorbent assay-inhibition tests with whole-cell antigens obtained from strains of serotype 1 through 12 of a pleuropneumoniae revealed that 5 mab-1 bound to only serotype-5 strains. the epitope recognized by 5mab-1 was a carbohydrate that was sensitive to periodate oxidation and resided on the structure of beta-1,6-linked d-galactose in an o-antige ...19921384404
comparative virulence of nad-dependent and nad-independent actinobacillus pleuropneumoniae strains.the virulence of a nad-independent actinobacillus pleuropneumoniae serotype 2 strain and nad-dependent serotype 2, 3 and 9 strains was compared under experimental conditions. hysterectomy-derived piglets were inoculated endobronchially with 50-500 cfu of these strains. all 23 piglets inoculated with the nad-dependent strains developed acute disease within 12 hours post inoculation. twenty-two of these piglets died within 24 hours after the first clinical signs. three of nine piglets inoculated w ...19921387754
partial denaturation of transthyretin is sufficient for amyloid fibril formation in vitro.amyloid diseases are caused by the self-assembly of a given protein into an insoluble cross-beta-sheet quaternary structural form which is pathogenic. an understanding of the biochemical mechanism of amyloid fibril formation should prove useful in understanding amyloid disease. toward this end, a procedure for the conversion of the amyloidogenic protein transthyretin into amyloid fibrils under conditions which mimic the acidic environment of a lysosome has been developed. association of a struct ...20051390650
retinal blood flow in diabetic retinopathy.(a) to report on the basic parameters of retinal blood flow in a population of diabetic patients with and without retinopathy and non-diabetic controls; (b) to formulate a haemodynamic model for the pathogenesis of diabetic retinopathy from this and other studies.20061393111
severe pulmonary hypertension without significant pulmonary parenchymal disease in a pediatric patient with acquired immunodeficiency syndrome. 20061395371
influence of actinobacillus pleuropneumoniae serotype 2 and its cytolysins on porcine neutrophil chemiluminescence.the effects of actinobacillus pleuropneumoniae serotype 2 and its metabolites on the oxidative activity of porcine neutrophils were studied by using a chemiluminescence technique. viable a. pleuropneumoniae stimulated the production of oxygen radicals by neutrophils. after having reached a peak value, the oxidative activity decreased until a total inhibition of the oxidative activity of the neutrophils was achieved. all effects were neutralized with homologous convalescent-phase pig sera which h ...19921398943
phagocytosis and killing of actinobacillus pleuropneumoniae by alveolar macrophages and polymorphonuclear leukocytes isolated from pigs.to study the cellular response of phagocytic cells to actinobacillus pleuropneumoniae, we investigated whether porcine alveolar macrophages (am) and polymorphonuclear leukocytes (pmn) are able to phagocytize and intracellularly kill a. pleuropneumoniae in vitro. bacterial cultivation methods of a. pleuropneumoniae were used to assess in vitro phagocytosis and the ability to kill. a specific-pathogen-free pig was killed, blood was collected, and pmn were isolated and counted. the am were isolated ...19921398997
study of acute intraoperative suprachoroidal hemorrhage.acute intraoperative suprachoroidal hemorrhage or effusion (aish) was studied in a prospective manner over the past three years. in a general cataract surgery population of 2,523 patient eyes, 15 (0.6%) experienced an aish. results of a specific surgical management technique are presented. surgical control of the aish consisted of immediate closure of the incision and application of pressure directly to the eye. this tamponades the effusion or hemorrhage, which allows coagulation and completion ...20061403754
[patient-controlled anesthesia in postoperative pain]. 20061410728
do central antiadrenergic actions contribute to the atypical properties of clozapine?full neuropharmacological understanding of the atypical antipsychotic agent clozapine remains elusive. antidopaminergic actions of most neuroleptics probably contribute to their antipsychotic benefits, but also to neurological side-effects. clinical evidence of abnormalities of dopamine (da) and serotonin (5-ht) in psychotic disorders is inconsistent, but there is substantial metabolic and post-mortem evidence for hyperactivity of noradrenaline (na). clozapine is only weakly antidopaminergic but ...19921418884
cellular changes in the bronchoalveolar lavage (bal) of pigs, following immunization by the enteral or respiratory route.normal young pigs were immunized by the oral or aerogenic route with the viable or inactivated lung-pathogenic bacterium actinobacillus (haemophilus) pleuropneumoniae. three weeks later the cellular composition as well as the lymphocyte subset composition of the bronchoalveolar space were examined by bal. lymphocytes in the lavage increased significantly, including cd4+ and cd8+ t cells. after oral immunization a dramatic increase of plasma cells and lymphoid blasts was found. among immunoglobul ...19921424278
activators and inhibitors of the motility of spermatozoa fucus serratus [corrected]. 20061426546
the nysb sund double mutant of dictyostelium discoideum is blocked in the acquisition of non-degradative resistance to the pea phytoalexin pisatin.dictyostelium discoideum amoebae can be induced to acquire a non-degradative resistance to the pea phytoalexin pisatin. the nysb sund double mutant is blocked in the acquisition of this resistance. using parasexual genetics it has been shown that sund is a recessive mutation linked to nysb on linkage group vi.19921426988
affinity for porcine respiratory tract mucus is found in some isolates of actinobacillus pleuropneumoniae.the ability of 17 actinobacillus pleuropneumoniae isolates representing serotypes 1, 2, 5, and 7, to adhere in vitro to porcine respiratory tract mucus was examined. adherence of bacteria to crude mucus preparations was evaluated by use of a dot-blot assay and an enzyme immunoassay. seventy per cent (12/17) of the isolates of a. pleuropneumoniae had affinity, to various degrees, for porcine respiratory tract mucus. no relationship was found between affinity for respiratory mucus and serotype, ha ...19921426994
recommendations for creation of modest level ii neonatal care facilities in india. 20061428139
drug resistant plasmid of actinobacillus pleuropneumoniae isolated from swine pleuropneumonia in thailand.actinobacillus pleuropneumoniae, isolated from the pneumonic lung tissue of pigs, was resistant to streptomycin, sulfadiazine and tetracycline. this isolate, ta5, possessed 2 plasmids (pta51 = 3.7 kb, pta52 = 6.2 kb); one of the plasmids (pta52) was coded for resistance to sm and sa. another isolate of a. pleuropneumoniae, ta8, also possessed 2 plasmids (pta81 = 3.7 kb, pta82 = 4.4 kb), with a plasmid (pta82) coded for resistance to sm and sa. all 4 plasmids were not transmissible and small.19921431383
quantifying by monoclonal antibodies of specific igg, igm and iga in the serum of minipigs experimentally infected with actinobacillus pleuropneumoniae.fifteen minipigs were infected intratracheally with three different doses (10(8), 10(5) or 5 x 10(3) colony forming units) of the reference strains of serotypes 2 or 4 of actinobacillus pleuropneumoniae, and three remained as controls. the titre of specific igg, igm and iga in the serum was measured weekly for 15 weeks with an indirect elisa using monoclonal antibodies specific for each isotype. igg attained the highest titres, igm lower and iga the lowest, being only detected in four animals. s ...19921439217
serological characterization of actinobacillus pleuropneumoniae strains isolated from pigs in quebec.a total of 3306 isolates of a. pleuropneumoniae originating from lung tissues of pigs that died of acute pleuropneumonia and 140 isolates recovered from tonsils or nasal cavities of apparently healthy pigs from chronically infected herds were serotyped. various serotyping methods, such as slide agglutination, tube agglutination, ring precipitation, coagglutination, immunodiffusion, indirect hemagglutination and counterimmunoelectrophoresis either alone or in combination were used. the techniques ...19921441197
nuclear-magnetic-resonance analysis of the capsular antigen of actinobacillus pleuropneumoniae serotype 9. its identity with the capsular antigen of escherichia coli k62 (k2ab), neisseria meningitidis serogroup h and pasteurella haemolytica serotype t15.the specific capsular antigen of actinobacillus pleuropneumoniae serotype 9 was characterized by one-dimensional and two-dimensional high-field nuclear-magnetic-resonance methods, and by chemical analyses, as a teichoic-acid-type polymer of a repeating unit having the structure [formula: see text] the basic polymer structure is identical to capsular antigens of neisseria meningitidis group h, escherichia coli k62 (k2ab) and pasteurella haemolytica serotype t15.19921446664
construction of a broad host range shuttle vector for gene cloning and expression in actinobacillus pleuropneumoniae and other pasteurellaceae.we have constructed a pair of broad host range expression vectors, pjff224-nx and pjff224-xn, based on plasmid rsf1010, which enable cloning and efficient expression of genes in actinobacillus pleuropneumoniae and pasteurella haemolytica and in escherichia coli. the vectors consist of the minimal autonomous replicon of the broad host range plasmid rsf1010 and a type ii chloramphenicol acetyl transferase gene for chloramphenicol resistance selection. in addition, they contain a gene expression ca ...19921448612
[extraction of an artery in rectal biopsy].a biopsy of a rectal carcinoma has led to the extraction of a 2.5 cm arterial specimen, followed by an arterial bleeding. the cause of this exceptional complication was the presence of atypical vessels reaching the rectal lumen due to tumor ulceration.20061449006
sequence analysis of the rob-1 beta-lactamase gene from actinobacillus pleuropneumoniae.the rob-1 beta-lactamase gene from actinobacillus pleuropneumoniae was cloned and sequenced. the structural gene encodes a 305 amino acid polypeptide. the rob-1 beta-lactamase gene sequence is identical to that derived from pasteurella haemolytica and only one amino acid different from that of haemophilus influenzae, suggesting that they are derived from the same ancestor, and transformed from one to another.19921455627
protective efficacy of conjugate vaccines against experimental challenge with porcine actinobacillus pleuropneumoniae.in an attempt to protect pigs against swine pleuropneumonia induced by actinobacillus pleuropneumoniae (spap) by neutralizing the effects of three virulence factors of a. pleuropneumoniae--the capsular polysaccharide (cp), the lipopolysaccharide (lps), and the hemolysin protein (hp)--two subunit conjugate vaccines were prepared by covalently coupling the cp to the hp and the lps to the hp. the cp, lps, and hp were isolated from a. pleuropneumoniae, strain 4074, serotype 1, and the protective eff ...19921455686
effect of citrate feeding on free radical induced changes in experimental urolithiasis.feeding calculi producing diet (cpd) to rats for 4 weeks produced calcium oxaltate stones. supplementation of sodium citrate to cpd (c-cpd) prevented stone formation. except oxalate, the excretion of calcium, phosphorus and magnesium was restored to normal in c-cpd fed rats. the cpd fed rats exhibited increase in glycolic acid oxidase (gao) and lactate dehydrogenase (ldh) activities and only gao activity was partially restored in c-cpd fed rats. kidney sub-cellular fractions of calculi producing ...19921459650
use of passive immunization for the production of monoclonal antibodies against actinobacillus pleuropneumoniae serotype 1,6 and 12.hyperimmune sera from balb/c mice immunized intraperitoneally (ip) with actinobacillus pleuropneumoniae serotype 2 were used for passive intraperitoneal (i.p.) immunization of balb/c mice. the immunized mice were subsequently immunized i.p. with a mixture of a. pleuropneumoniae serotypes 1, 6 and 12. numerous monoclonal antibodies specific for serotypes 1, 6 and 12 were obtained. using this immunization scheme antibodies can be obtained against specific antigens from closely related bacteria.19921474261
chemical systems for delivery of antiepileptic drugs to the central nervous system.the chemical delivery system (cds) approach, a recently developed procedure conceived to enhance the specific central nervous system (cns) uptake of drugs, has been applied to several antiepileptic agents. cdss based on dihydropyridine<-->pyridinium salt type redox targetors, reversibly linked to the drug, were designed, synthesized and tested for some traditional (phenytoin, valproate) and potential (stiripentol) antiepileptic drugs, as well as some compounds (gaba, adenosine) with important ro ...20061478193
[occurrence and drug resistance of bacteria pathogenic to the lungs from autopsy material of swine].eight hundred and fifty-four piglets which died or were euthanized due to pneumonia or rhinitis atrophicans, were investigated during the period of 1986-1990. of the animals, 569 showed bronchopneumonia, 218 had pleuritis, pericarditis and peritonitis, 165 had rhinitis atrophicans, 58 pleuropneumonia, and 9 animals had fibrinous pneumonia. pasteurella multocida, haemophilus parasuis, bordetella bronchiseptica, actinobacillus pleuropneumoniae and pasteurella haemolytica were isolated in 59.1%, 29 ...19921481214
representation of clinical data using snomed iii and conceptual graphs.none of the coding schemes currently contained within the unified medical language system (umls) is sufficiently expressive to represent medical progress notes adequately. some coding schemes suffer from domain incompleteness, others suffer from the inability to represent modifiers and time references, and some suffer from both problems. the recently released version of the systematized nomenclature of medicine (snomed iii) is a potential solution to the data-representation problem because it is ...20061482897
acrylic resin finger prosthesis.a technique is described for the prosthetic replacement of two fingers using acrylic resin. retention was obtained using finger rings. the stages of the technique are outlined. advantages and disadvantages are briefly stated.20061484252
[practice replaced by theory]. 20061489591
characterisation of the igg response to cell proteins of coagulase-negative staphylococci in hydrocephalus shunt infections. 19921489739
a comparison of pain perceptions in women with fibromyalgia and rheumatoid arthritis: relationship to depression and pain extent.two studies were conducted to characterize the pain of fibromyalgia syndrome (fms); to compare it to rheumatoid arthritis (ra) pain; and to examine the relationships between depression, pain extent, and pain description. two methods of administering the mcgill pain questionnaire (mpq) were used. when the mpq was administered in the standard manner, fms pain could not be distinguished from ra pain. when participants were allowed to select as many words from an adapted mpq as they wished, signific ...20061489768
serotype specificity of immunological assays for the capsular polymer of actinobacillus pleuropneumoniae serotypes 1 and 9.the cross-reactivity of the purified polysaccharides of actinobacillus pleuropneumoniae serotypes 1 and 9 were examined using a variety of highly sensitive assays, such as radioimmunoassay, latex agglutination, enzyme-linked immunosorbent assay (elisa), and immunoblotting. in addition, conventional immunodiffusion was included for comparison. latex agglutination, utilizing affinity-purified igg to capsule, was also used to serotype whole cells. agglutination or precipitation tests (radioimmunoas ...19921496808
stimulation and suppression of the oxygenation activity of porcine pulmonary alveolar macrophages by actinobacillus pleuropneumoniae and its metabolites.the effects of actinobacillus (haemophilus) pleuropneumoniae and its metabolites on the oxygenation activity of porcine pulmonary alveolar macrophages (pam) were studied, using a chemiluminescence technique. actinobacillus pleuropneumoniae strains of serotypes 2, 3, and 9 in a dose of 1, 10, and 100 colony-forming units/macrophage first stimulated the oxygen radical production of pam. after having reached a peak value, oxygenation activity decreased, finally resulting in total suppression of pam ...19921497179
pleuropneumonia caused by actinobacillus pleuropneumoniae biotype 2 in growing and finishing pigs.actinobacillus pleuropneumoniae biotype 2 was isolated in pure culture or as the predominant isolate from the lungs of 9 growing and finishing pigs with pleuropneumonia. gross and microscopic lesions resembled those caused by a. pleuropneumoniae biotype 1 serotypes (nos. 1, 5, and 7) traditionally seen in the united states. the overall mortality rate for growing and finishing pigs on this 1,200-sow farrow-to-finish farm ranged from 0.37% to 0.84% per month from july 1990 to february 1991, and mo ...19921515488
[minimally invasive interventions in solitary liver cysts].large intrahepatic cysts need surgical intervention when becoming symptomatic. in case of non-parasitic solitary cysts different surgical treatments are available. we performed three different minimal-invasive procedures in 8 patients. good results were obtained by laparoscopic extended deroofing of the cyst. indications and technical performance are presented.19941534532
rapid purification of a 110-kilodalton hemolysin of actinobacillus pleuropneumoniae by monoclonal antibody-affinity chromatography.an efficient, single-step method for purification of the 110-kilodalton (kda) hemolysin of actinobacillus pleuropneumoniae was developed. an immunoaffinity column was made by cross-linking murine monoclonal antibody 8c2 to the 110-kda hemolysin of a pleuropneumoniae strain j45 serotype 5 to protein a-agarose beads. purified hemolysin with high hemolytic activity was obtained after washing the column with phosphate-buffered saline solution, and eluting the hemolysin with 50 mm diethylamine, ph 11 ...19921539917
prophylaxis against non-steroidal induced upper gastrointestinal side effects. 20061540030
immune complexes in ankylosing spondylitis.immune complexes have been reported in ankylosing spondylitis (as) and may implicate infectious agents. serum samples from 49 patients with as were assayed for immune complexes by polyethylene glycol precipitation, followed by radial immunodiffusion and pepsinogen binding immunoassay. both methods showed increases in iga containing immune complexes, which correlated with serum iga and with iga rheumatoid factor concentrations, but did not show increases in other immune complex components. increa ...19921540045
effects of cardioplegic solutions and their components on human saphenous vein contractility.cardioplegic solution administration into the vein graft is an established method to ensure cardioplegic distribution beyond coronary artery stenoses. the ultrastructural demonstration of severe endothelial damage after cardioplegic exposure suggests that intravenous cardioplegic administration can contribute to early and late graft thrombosis. the direct effect on human saphenous vein contractility of three cardioplegic solutions and their components was compared. a solution with 30 mmol/l k+ a ...19921540064
cloning and expression of a transferrin-binding protein from actinobacillus pleuropneumoniae.an expression library was constructed from actinobacillus pleuropneumoniae serotype 7. escherichia coli transformants expressing recombinant proteins were identified by immunoscreening with porcine convalescent serum. one transformant expressing a 60-kda protein (60k protein) in aggregated form was identified. serum raised against the recombinant protein recognized a polypeptide with an indistinguishable electrophoretic mobility in the a. pleuropneumoniae wild type after iron-restricted growth o ...19921541562
effects of actinobacillus pleuropneumoniae hemolysin on porcine neutrophil function.in an attempt to gain insight into the events that take place during actinobacillus pleuropneumoniae infection, the present study was designed to ascertain the effects of bacterial toxicity on porcine neutrophil functions and viability. incubation of phagocytes (2 x 10(6)) with opsonized a. pleuropneumoniae 4074 (2 x 10(7) cfu) resulted in phagocytic uptake of less than or equal to 4%. at the same bacterium-to-phagocyte ratio, levels of lactate dehydrogenase activity of 74 and 81% were detected ...19921548079
identification of a second hemolysin (hlyii) in actinobacillus pleuropneumoniae serotype 1 and expression of the gene in escherichia coli.hemolysin genes of the reference strains of actinobacillus pleuropneumoniae serotypes 1 and 2 were identified, cloned, and expressed in escherichia coli by using polymerase chain reaction amplification with oligonucleotides derived from the dna sequence of the corresponding appa gene from a. pleuropneumoniae serotype 5. the three genes from serotypes 1, 2, and 5 have identical restriction maps and appear to encode a hemolysin which was previously identified in serotype 2 and designated hlyii. ge ...19921548089
clonal analysis of the actinobacillus pleuropneumoniae population in a geographically restricted area by multilocus enzyme electrophoresis.the genetic diversity among 250 isolates of actinobacillus pleuropneumoniae from lungs of pigs with pleuropneumonia and from tonsils of apparently healthy pigs at slaughter was estimated by multilocus enzyme electrophoresis. the danish strains were derived from both specific-pathogen-free and conventional herds. sixty-six percent of the isolates belonged to three electrophoretic types (ets) of a total of 37 ets detected. while five biotype 2 isolates constituted a separate et closely related to ...19921551979
11 beta-amidoalkyl estradiols, a new series of pure antiestrogens.in order to find new antiestrogens, devoid of any agonistic activity, a series of 11 beta-amidoalkyl estradiols were prepared. these compounds have been studied in comparison with tamoxifen (tam): in vitro, for their relative binding affinities (rba) for mouse and mcf-7 estrogen receptors (er) and for their antiproliferative effect on mcf-7 (estradiol or egf/pdgf stimulated) and ly2 human breast cancer cell lines; in vivo, for their uterotrophic/antiuterotrophic activities in the mouse and for t ...19921562531
in situ hybridization of ovalbumin mrna in the chick oviduct reveals target cell specificity for estrogen and progesterone.an in situ hybridization method using paraffin-embedded sections was used to characterize the chicken oviduct cells synthesizing ovalbumin mrna due to the action of estrogen and progesterone. the cytodifferentiation of the oviduct cells was induced by 17 beta-estradiol administration to newly hatched female chicks. to avoid possible effect of estrogen on the action of progesterone the chicks were withdrawn from the estrogen by six days withdrawal period without hormone treatment. ovalbumin mrna ...19921562535
how the potency of the steroid ru486 is related to p450 activities induced by dexamethasone and phenobarbital in rat hepatoma cells.in a previous work on rat liver microsomes, we demonstrated that cytochrome p450 isozymes (p450) are engaged in the metabolism of ru486. in order to study the underlying mechanism at the molecular level, our investigations were shifted to a simplified system of cultured hepatoma cells which present a dissociation in the expression of distinct p450 coding genes. our results show that fao cells represent a convenient model to study both: (i) the degradation of ru486. forms iib1,2 and iic7, which a ...19921562537
viability of actinobacillus pleuropneumoniae in frozen pig lung samples and comparison of different methods of direct diagnosis in fresh samples.a comparative study on different methods of diagnosis of actinobacillus pleuropneumoniae from both fresh and frozen pig lungs is described. a total of 196 lung tissues with pneumonic lesions were examined for culture isolation on chocolate blood agar, as well as for antigen detection by means of the coagglutination test, the immunodiffusion test and the indirect elisa. these samples were subsequently frozen for 1 yr and then they were recultured. a. pleuropneumoniae was recovered from fresh lung ...19921563263
[recurrent spontaneous miscarriage].spontaneous recurrent miscarriages still represent a poorly defined entity. it is commonly accepted that they include early (1st trimester) spontaneous abortion thus excluding those related to well documented chromosomal, anatomical, hormonal or autoimmune causes such as sle or anticardiolipin syndrome. there is also a general consensus based upon historical series to require at least three consecutive miscarriages. in a series of 58 consecutive couples with recurrent miscarriages studied at the ...20061594822
ventriculopleural shunting used as a temporary diversion.due to the limited absorptive capacity of the pleural cavity, infants and young children are not generally ideal candidates for ventriculopleural shunts. we report using chest cavities as alternate for temporary diversion of csf in a young child. venous access to the cervical region could not be utilized because of scarring from previous procedures, while peritoneal access was contraindicated due to repeated pseudocyst formation. pleural effusions were removed by thoracentesis when necessary, an ...20061595400
the ventriculofugal arteries. 20061595488
equanimity. 20061595502
identification of a rob-1 beta-lactamase in haemophilus ducreyi.a collection of 100 clinical isolates of haemophilus ducreyi from thailand were all found to harbor a 5.4-kb plasmid, designated pth126, which was shown to contain the bla rob-1 gene. restriction enzyme analysis and dna-dna hybridization studies confirmed that pth126 was similar to the rob-1 beta-lactamase plasmid pvm105 from actinobacillus pleuropneumoniae. in approximately one-half of the isolates, pth126 was found together with phd131, which mediates tem-1 beta-lactamase production.19921605612
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