Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| description of a bhk/21 cell line persistently infected with junin virus: its use in diagnostic procedures. | a bhk/21 cell line persistently infected by an arenavirus is described. during four consecutive passages, 30-45% of the cells showed granular cytoplasmic antigen by indirect immunofluorescence, employing both argentine hemorrhagic fever convalescent sera and sera from animals immunized with junin virus. virus isolated from the cells killed suckling mice but not adult mice and protected guinea pigs against further challenge with the virulent prototype strain of junin virus. neutralization tests s ... | 1980 | 6263820 |
| description of a bhk/21 cell line persistently infected with junin virus: its use in diagnostic procedures. | a bhk/21 cell line persistently infected by an arenavirus is described. during four consecutive passages, 30-45% of the cells showed granular cytoplasmic antigen by indirect immunofluorescence, employing both argentine hemorrhagic fever convalescent sera and sera from animals immunized with junin virus. virus isolated from the cells killed suckling mice but not adult mice and protected guinea pigs against further challenge with the virulent prototype strain of junin virus. neutralization tests s ... | 1980 | 6263820 |
| [properties of the interfering particles generated by junin virus in vero cells]. | 1981 | 6267411 | |
| [protection induced in guinea pigs by the variant xj0 of junin virus]. | 1981 | 6267412 | |
| [protection induced in guinea pigs by the variant xj0 of junin virus]. | 1981 | 6267412 | |
| [immunologic markers of attenuation in guinea pigs infected with strains or variants of junin virus]. | 1981 | 6267413 | |
| [immunologic markers of attenuation in guinea pigs infected with strains or variants of junin virus]. | 1981 | 6267413 | |
| [tissue selectivity and virulence indicators of 3 strains of junin virus]. | 1981 | 6268927 | |
| immunogenicity of a/ussr (h1n1) subunit vaccine in unprimed young adults. | a clinical and serological study was performed on 267 of 636 volunteers vaccinated against argentine hemorrhagic fever with the xjcl3 attenuated strain of junin virus seven to nine years earlier, in order to determine their long-term evolution. this study included a clinical examination, a chest roentgenogram, an electrocardiogram, and the following laboratory determinations: white and red cell count, number of platelets, hematocrit, hemoglobin, sedimentation rate (katz index), urea, nitrogen, g ... | 1981 | 6270279 |
| argentine hemorrhagic fever: a biologic marker. | mortality rates and viral replication in blood and brains of wistar rats between 6 h and 26 days of age inoculated with two strains of junin virus of different virulence were compared. viral growth curves in brains showed no differences between strains. however, differences in mortality rates were significant among rats between 1 and 3 days of age. when the intracerebral (i.c.) route was used, high mortality rates were induced by the attenuated xjc13 strain and low mortality rates were induced b ... | 1981 | 6271705 |
| cell-mediated immunity and lymphocyte populations in experimental argentine hemorrhagic fever (junín virus). | guinea pigs infected with the xj prototype strain of junín virus reproduce the main features of argentine hemorrhagic fever, showing hemorrhages, leukothrombocytopenia, and focal lymphoid tissue necrosis. viral lymphotropism is shown by the presence of viral antigens, severe cytopathic effect, and high virus titers in lymphoid organs. a pronounced depression of humoral immune response to sheep erythrocytes as well as to the virus is described. this study was carried out to determine whether cell ... | 1981 | 6273314 |
| cell-mediated immunity and lymphocyte populations in experimental argentine hemorrhagic fever (junín virus). | guinea pigs infected with the xj prototype strain of junín virus reproduce the main features of argentine hemorrhagic fever, showing hemorrhages, leukothrombocytopenia, and focal lymphoid tissue necrosis. viral lymphotropism is shown by the presence of viral antigens, severe cytopathic effect, and high virus titers in lymphoid organs. a pronounced depression of humoral immune response to sheep erythrocytes as well as to the virus is described. this study was carried out to determine whether cell ... | 1981 | 6273314 |
| in vitro infection of murine macrophages with junin virus. | mouse peritoneal macrophages were successfully infected with two strains of junin virus producing high titers with no apparent cell damage. infected cultures survived longer than noninfected cultures. the pattern of virus release suggested a persistent infection. virus replication was delayed in macrophages from mice previously immunized with junin virus. these results support the opinion that macrophages are targets for virus replication in vivo infections. | 1982 | 6274800 |
| cross-protection in nonhuman primates against argentine hemorrhagic fever. | the susceptibility of the marmoset callithrix jacchus to tacaribe virus infection was investigated to perform cross-protection studies between junin and tacaribe viruses. five marmosets inoculated with tacaribe virus failed to show any signs of disease, any alterations in erythrocyte, leukocyte, reticulocyte, and platelet counts or any changes in hematocrit or hemoglobin values. no tacaribe virus could be recovered from blood at any time postinfection. anti-tacaribe neutralizing antibodies appea ... | 1982 | 6276301 |
| cross-protection in nonhuman primates against argentine hemorrhagic fever. | the susceptibility of the marmoset callithrix jacchus to tacaribe virus infection was investigated to perform cross-protection studies between junin and tacaribe viruses. five marmosets inoculated with tacaribe virus failed to show any signs of disease, any alterations in erythrocyte, leukocyte, reticulocyte, and platelet counts or any changes in hematocrit or hemoglobin values. no tacaribe virus could be recovered from blood at any time postinfection. anti-tacaribe neutralizing antibodies appea ... | 1982 | 6276301 |
| diagnosis of junin virus in cell cultures by immunoperoxidase staining. | 1981 | 6277286 | |
| new attenuation marker for junin virus based on immunologic responses of guinea pigs. | a new attenuation marker to distinguish a virulent strain (xjjv) from an attenuated strain (xjc13jv or xjojv) of junin virus by means of the humoral and cellular responses to unrelated antigens was studied in guinea pigs. strain xjjv suppressed the humoral immune response, as shown by the lower titers of precipitating antibody to ovalbumin. the concomitant decrease in serum complement level contributed to a milder arthus cutaneous reactivity. in contrast, the attenuated strains did not decrease ... | 1982 | 6278027 |
| new attenuation marker for junin virus based on immunologic responses of guinea pigs. | a new attenuation marker to distinguish a virulent strain (xjjv) from an attenuated strain (xjc13jv or xjojv) of junin virus by means of the humoral and cellular responses to unrelated antigens was studied in guinea pigs. strain xjjv suppressed the humoral immune response, as shown by the lower titers of precipitating antibody to ovalbumin. the concomitant decrease in serum complement level contributed to a milder arthus cutaneous reactivity. in contrast, the attenuated strains did not decrease ... | 1982 | 6278027 |
| [a new plaque assay for the quantification of junin virus]. | 1981 | 6287522 | |
| [effect of experimental infection of pregnant guinea pigs with junin virus]. | 1981 | 6287523 | |
| [effect of experimental infection of pregnant guinea pigs with junin virus]. | 1981 | 6287523 | |
| [response of calomys musculinus to experimental infection with junin virus]. | 1982 | 6290832 | |
| [response of calomys musculinus to experimental infection with junin virus]. | 1982 | 6290832 | |
| junin virus infection in genetically athymic mice. | the progression of junin virus infection was studied in congenitally athymic mice. immunocompetent littermates were used as infected controls. as expected, the latter developed lethal encephalitis, with viremia and considerable viral replication in the brain. the mortality rate was almost 100%; the few surviving controls exhibited high serum neutralizing antibody levels and a total absence of virus in blood and brain. in contrast, nude mice did not contract the disease; all survived with persist ... | 1983 | 6298141 |
| cytogenetic effect of two strains of junin virus in the guinea pig. | the cytogenetic effect of two strains of junin virus on bone marrow chromosomes of the guinea pig was studied. animals infected with the attenuated strain xj-cl3 showed no differences from control animals. guinea pigs inoculated with the pathogenic strain xj exhibited a significant increase of abnormal cells, chromatid breaks, and chromosome fragments. the clastogenic ability of the xj strain is similar to the reported effect of other viruses, while the xj-cl3 strain does not appear to be clasto ... | 1983 | 6298143 |
| cytogenetic effect of two strains of junin virus in the guinea pig. | the cytogenetic effect of two strains of junin virus on bone marrow chromosomes of the guinea pig was studied. animals infected with the attenuated strain xj-cl3 showed no differences from control animals. guinea pigs inoculated with the pathogenic strain xj exhibited a significant increase of abnormal cells, chromatid breaks, and chromosome fragments. the clastogenic ability of the xj strain is similar to the reported effect of other viruses, while the xj-cl3 strain does not appear to be clasto ... | 1983 | 6298143 |
| junin virus infection of callithrix jacchus: pathologic features. | infection of callithrix jacchus, a new world primate, with the prototype strain of junin virus produced a severe disease. the animals developed multifocal hemorrhages and characteristic microscopic lesions such as meningoencephalitis, interstitial pneumonia, lymphocytic depletion of lymphatic tissue, hepatocytic necrosis, and a variable decrease in bone marrow cellularity. high virus concentrations correlated with lesions, and with the presence of viral antigenic determinants as revealed by immu ... | 1983 | 6301303 |
| junin virus infection of callithrix jacchus: pathologic features. | infection of callithrix jacchus, a new world primate, with the prototype strain of junin virus produced a severe disease. the animals developed multifocal hemorrhages and characteristic microscopic lesions such as meningoencephalitis, interstitial pneumonia, lymphocytic depletion of lymphatic tissue, hepatocytic necrosis, and a variable decrease in bone marrow cellularity. high virus concentrations correlated with lesions, and with the presence of viral antigenic determinants as revealed by immu ... | 1983 | 6301303 |
| congenital and perinatal infection with junin virus in guinea pigs. | junin virus infection in guinea pigs is known to be similar to human argentine hemorrhagic fever (ahf). the guinea pig was chosen as a model for transplacental transmission of junin virus, as both guinea pig and man have a similar placental structure. pregnant guinea pigs were infected with the pathogenic xj strain of junin virus intramuscularly route at different stages of pregnancy. the group infected during the last third of pregnancy produced 16 newborn, but mortality reached 100%: 18% were ... | 1983 | 6302220 |
| congenital and perinatal infection with junin virus in guinea pigs. | junin virus infection in guinea pigs is known to be similar to human argentine hemorrhagic fever (ahf). the guinea pig was chosen as a model for transplacental transmission of junin virus, as both guinea pig and man have a similar placental structure. pregnant guinea pigs were infected with the pathogenic xj strain of junin virus intramuscularly route at different stages of pregnancy. the group infected during the last third of pregnancy produced 16 newborn, but mortality reached 100%: 18% were ... | 1983 | 6302220 |
| [infection of new world primates with junin virus: iii. saimiri sciureus]. | 1982 | 6302438 | |
| [infection of new world primates with junin virus: iii. saimiri sciureus]. | 1982 | 6302438 | |
| [action of cyclophosphamide on intracerebral infection of guinea pigs with junin virus]. | 1982 | 6302439 | |
| [action of cyclophosphamide on intracerebral infection of guinea pigs with junin virus]. | 1982 | 6302439 | |
| immunoperoxidase study of astrocytic reaction in junín virus encephalomyelitis of mice. | intracerebral inoculation of strain xj clon 3 of junín virus into 1-2-day-old mice resulted in the appearance of viral antigen, detectable by means of the peroxidase antiperoxidase (pap) technique, within the cytoplasm of the neurons of the cerebral cortex, the basal nuclei, cerebellum, pons, medulla, spinal cord, and spinal ganglia; junín antigen was likewise observed, although to a lesser extent, in the cytoplasm of astrocytes. the viral antigen was found in highest concentration at the cytopl ... | 1983 | 6303038 |
| [the nursing guinea pig as an indicator of junin virus neurovirulence]. | 1982 | 6306384 | |
| [the nursing guinea pig as an indicator of junin virus neurovirulence]. | 1982 | 6306384 | |
| [infection of the lung with junin virus in experimental argentinian hemorrhagic fever of the guinea pig]. | 1983 | 6308382 | |
| [infection of the lung with junin virus in experimental argentinian hemorrhagic fever of the guinea pig]. | 1983 | 6308382 | |
| protection against a pathogenic strain of junin virus by mucosal infection with an attenuated strain. | in order to determine the degree of mucosal infectivity of the attenuated xjcl3 strain of junin virus, guinea pigs were orally or nasally inoculated. infectivity was 85% for the oral and 100% for the nasal route, as detected by death or serum antibody development. the presence of serum antibodies was closely associated with resistance to challenge with the xj pathogenic strain, which killed 100% of controls when inoculated by the parenteral or nasal route. however, mortality rates after mucosal ... | 1983 | 6309026 |
| protection against a pathogenic strain of junin virus by mucosal infection with an attenuated strain. | in order to determine the degree of mucosal infectivity of the attenuated xjcl3 strain of junin virus, guinea pigs were orally or nasally inoculated. infectivity was 85% for the oral and 100% for the nasal route, as detected by death or serum antibody development. the presence of serum antibodies was closely associated with resistance to challenge with the xj pathogenic strain, which killed 100% of controls when inoculated by the parenteral or nasal route. however, mortality rates after mucosal ... | 1983 | 6309026 |
| in vivo replication of pathogenic and attenuated strains of junin virus in different cell populations of lymphatic tissue. | lymphatic tissue is one of the main sites for replication of junin virus. to characterize which cells are involved in that replication, the presence of junin virus in purified populations of macrophages and dendritic cells from the spleens of guinea pigs infected with pathogenic and attenuated strains was investigated by immunofluorescence and intracerebral inoculation into newborn mice. the pathogenic strain was present both in macrophages and in dendritic cells, but the attenuated strain selec ... | 1983 | 6309667 |
| in vivo replication of pathogenic and attenuated strains of junin virus in different cell populations of lymphatic tissue. | lymphatic tissue is one of the main sites for replication of junin virus. to characterize which cells are involved in that replication, the presence of junin virus in purified populations of macrophages and dendritic cells from the spleens of guinea pigs infected with pathogenic and attenuated strains was investigated by immunofluorescence and intracerebral inoculation into newborn mice. the pathogenic strain was present both in macrophages and in dendritic cells, but the attenuated strain selec ... | 1983 | 6309667 |
| pathogenicity of an attenuated strain (xjcl3) of junin virus. morphological and virological studies in experimentally infected guinea pigs. | infection of guinea pigs with an attenuated strain of junin virus (jv) produced 16% mortality between days 17 and 27 postinfection (p.i.). the morphological study showed a marked pancreatitis between days 6 and 23 p.i. and meningoencephalitis between days 17 and 20 p.i. in a large proportion of the animals. these lesions were coincident with the presence of jv antigenic determinants in the pancreatic acinar cells, neurons and blood vessels of the brain. infectious virus could be isolated from ly ... | 1983 | 6317604 |
| pathogenicity of an attenuated strain (xjcl3) of junin virus. morphological and virological studies in experimentally infected guinea pigs. | infection of guinea pigs with an attenuated strain of junin virus (jv) produced 16% mortality between days 17 and 27 postinfection (p.i.). the morphological study showed a marked pancreatitis between days 6 and 23 p.i. and meningoencephalitis between days 17 and 20 p.i. in a large proportion of the animals. these lesions were coincident with the presence of jv antigenic determinants in the pancreatic acinar cells, neurons and blood vessels of the brain. infectious virus could be isolated from ly ... | 1983 | 6317604 |
| junin virus activity in two rural populations of the argentine hemorrhagic fever (ahf) endemic area. | to determine the prevalence of inapparent infection with junin virus among the rural population and its relation to the clinical disease, a serological study was carried out in two zones of the endemic area of argentine hemorrhagic fever (ahf). from the first appearance of ahf in the zones (1963) and the moment of the survey (1977), 14 years had passed. a total of 695 serum samples were obtained, 540 from córdoba and 155 from buenos aires. of the 695 serum samples, 83 were positive for neutraliz ... | 1983 | 6317799 |
| [chronic infection with junin virus in the rat. pathological and virological studies]. | 1983 | 6318010 | |
| [viral persistence and absence of brain lesions in congenitally athymic mice infected with junin virus]. | 1983 | 6318011 | |
| [different clinical manifestations of suckling mice inoculated with various strains of junin virus]. | 1983 | 6325846 | |
| [neuropathology of the junin virus in the guinea pig: new contributions]. | 1983 | 6325848 | |
| [neuropathology of the junin virus in the guinea pig: new contributions]. | 1983 | 6325848 | |
| protection conferred against junin virus infection in rats. | 2-day-old wistar rats intracerebrally infected with the xjc13 strain of virus exhibited a 5% survival rate which rose to 71% after immune serum treatment. brain viral titers were relatively unaffected by this treatment. histologic studies showed necrosis in the cerebellum and brain cortex with mononuclear cell infiltration in both treated and nontreated groups. beginning on day 16 postinfection, however, intracerebral perivascular gliosis foci and mild meningeal congestion were minimal in the tr ... | 1984 | 6327562 |
| transplacental infection in guinea pigs inoculated with an attenuated strain of junin virus. | transplacental infection by the attenuated xjc13 strain of junin virus (jv) in the guinea pig model was evaluated. 5 pregnant guinea pigs were infected intramuscularly at 45 +/- 3 days of pregnancy. 4 animals were killed at 14 days postinfection (p.i.), and 1 was sacrificed at 137 days p.i. at the end of its second pregnancy. evidence of jv was obtained by vero cell cocultivation in all 14 fetuses harvested (brain and/or spleen) and in 10 of 11 placentas. the results strongly suggest that the at ... | 1984 | 6327563 |
| transplacental infection in guinea pigs inoculated with an attenuated strain of junin virus. | transplacental infection by the attenuated xjc13 strain of junin virus (jv) in the guinea pig model was evaluated. 5 pregnant guinea pigs were infected intramuscularly at 45 +/- 3 days of pregnancy. 4 animals were killed at 14 days postinfection (p.i.), and 1 was sacrificed at 137 days p.i. at the end of its second pregnancy. evidence of jv was obtained by vero cell cocultivation in all 14 fetuses harvested (brain and/or spleen) and in 10 of 11 placentas. the results strongly suggest that the at ... | 1984 | 6327563 |
| persistence of attenuated junin virus strains in guinea pigs infected by im or ic routes. | 1984 | 6330233 | |
| persistence of attenuated junin virus strains in guinea pigs infected by im or ic routes. | 1984 | 6330233 | |
| [persistence of junin virus in diploid human cells]. | 1983 | 6664292 | |
| [prevalence of subclinical junin virus infection in a select population of an endemic area of argentinian hemorrhagic fever]. | 1982 | 7132697 | |
| [congenital infection by junin virus in guinea pigs]. | 1981 | 7321861 | |
| [congenital infection by junin virus in guinea pigs]. | 1981 | 7321861 | |
| [new findings on junin virus infection in rodents inside and outside the endemic area of hemorrhagic fever in argentina]. | in conjunction with field trials for a vaccine against argentine hemorrhagic fever (ahf), small mammals were trapped during a 28-month period (1 november 1987 to 13 march 1990) in 3 epidemiologically defined areas of the central argentine pampas: northern and central buenos aires provinces were included in the ahf "historic" area, where the disease was common 15-20 years ago, but case rates are currently low; southern santa fe province is the current high-incidence area for ahf; the nonendemic a ... | 1991 | 7476104 |
| rapid diagnosis of argentine hemorrhagic fever by reverse transcriptase pcr-based assay. | argentine hemorrhagic fever (ahf) is an endemo-epidemic disease caused by junín virus. this report demonstrates that a reverse transcriptase (rt) pcr-based assay developed in our laboratory to detect junín virus in whole blood samples is sensitive and specific. the experiments were conducted in a double-blinded manner using 94 clinical samples collected in the area in which ahf is endemic. the rt-pcr-based assay was compared with traditional methodologies, including enzyme-linked immunosorbent a ... | 1995 | 7542268 |
| [influence] of enzymatic treatment on junin virus--vero cells interaction]. | the chemical nature of cellular structures involved in the attachment of junin virus (wild type xjc13 and host range mutant cl 67) to vero cells was investigated. enzyme treatment of cells before virus infection indicated that whereas lipids are not directly involved in virus attachment, cellular proteins play a significant role in early interaction with jv. moreover aromatic residues, leucine and basic amino acid seem to actively participate in this interaction with different affinity for the a ... | 1995 | 7568866 |
| early markers of blood coagulation and fibrinolysis activation in argentine hemorrhagic fever. | junin virus, an arenaviridae, is the etiological agent of argentine hemorrhagic fever. in addition to thrombocytopenia, patients present several alterations in both the blood coagulation and the fibrinolytic system, but diffuse intravascular coagulation could not be demonstrated. to investigate further the activation status of the two systems, levels of thrombin-antithrombin complexes (tat), prothrombin fragment 1 + 2, protein c, total and free protein s, c4bbp, antithrombin iii, t-pa, pai-1 and ... | 1995 | 7667817 |
| neural spread of junin virus in intraperitoneally inoculated rats. | on the basis of an already demonstrated junín virus (jv) neural route after peripheral footpad infection of newborn rats, here we attempted to determine the viral pathway following intraperitoneal inoculation. as from the 2nd week post-infection, neurological disease developed reaching 84% mortality at 30 days. immunoperoxidase labeling of viral antigen, concomitantly with infectivity assays and histological examination, was carried out in serially harvested samples. whenever infectivity was det ... | 1994 | 7715431 |
| [present status of zoonotic hemorrhagic fevers of south america]. | since 1958, the geographical distribution of argentine hemorrhagic fever (ahf) has especially extended non only into the province of buenos aires but also towards the provinces of santa fe and cordoba, leading to an estimated population at risk of about 1.2 m inhabitants. recent epidemiological field studies has confirmed the major role of calomys musculinus and c. laucha rodents in both transmission to man and conservation of junin virus in nature. however, the human infection may result essent ... | 1993 | 7819800 |
| [present status of zoonotic hemorrhagic fevers of south america]. | since 1958, the geographical distribution of argentine hemorrhagic fever (ahf) has especially extended non only into the province of buenos aires but also towards the provinces of santa fe and cordoba, leading to an estimated population at risk of about 1.2 m inhabitants. recent epidemiological field studies has confirmed the major role of calomys musculinus and c. laucha rodents in both transmission to man and conservation of junin virus in nature. however, the human infection may result essent ... | 1993 | 7819800 |
| calomys musculinus, the natural reservoir of argentine haemorrhagic fever, is a semipermissive host for a mouse-attenuated mutant of junin virus. | c167, a mouse-attenuated strain of junin virus derived from the xjc13 strain, also displayed reduced virulence for the south american cricetid calomys musculinus, a natural reservoir of this virus in nature. intracerebral inoculation of c. musculinus with 500 pfu of c167 produced only 25% mortality, whereas the parental xjc13 killed 85% of the animals. the attenuation of c167 for this cricetid was lower than for albino mice. the multiplication of c167 in c. musculinus-derived embryo or kidney fi ... | 1994 | 7839011 |
| calomys musculinus, the natural reservoir of argentine haemorrhagic fever, is a semipermissive host for a mouse-attenuated mutant of junin virus. | c167, a mouse-attenuated strain of junin virus derived from the xjc13 strain, also displayed reduced virulence for the south american cricetid calomys musculinus, a natural reservoir of this virus in nature. intracerebral inoculation of c. musculinus with 500 pfu of c167 produced only 25% mortality, whereas the parental xjc13 killed 85% of the animals. the attenuation of c167 for this cricetid was lower than for albino mice. the multiplication of c167 in c. musculinus-derived embryo or kidney fi ... | 1994 | 7839011 |
| cosalane analogues with enhanced potencies as inhibitors of hiv-1 protease and integrase. | several new analogues of the novel anti-hiv agent cosalane have been synthesized and evaluated as inhibitors of hiv-1 integrase and protease, hiv-1 replication, hiv-1 and hiv-2 cytopathicity, hiv-1- and hiv-2-mediated syncytium formation, and cytopathicity of a variety of human pathogenic viruses. the congeners displayed enhanced potencies relative to cosalane itself as inhibitors of hiv-1 integrase and protease. the two most potent analogues against hiv-1 integrase displayed ic50 values of 2.2 ... | 1995 | 7853337 |
| intracellular processing and transport of junin virus glycoproteins influences virion infectivity. | the influence of glycoprotein processing, cleavage and transport on junin virus (jv) infectivity was investigated using monensin combined with lectin binding assays. yields of extracellular virus were more significantly reduced than cell-associated virus, indicating that monensin inhibited the transport of infectious virus to the extracellular space on a late stage of the replicative cycle. shown by lectin reactivity and immunoprecipitation, the intracellular processing of jv glycoproteins invol ... | 1994 | 7856317 |
| prevalence of infection with junin virus in rodent populations in the epidemic area of argentine hemorrhagic fever. | we report the results of indirect fluorescent antibody screening for antibody to junin virus in 1,101 sera from small mammals captured on two mark-recapture grids in the epidemic area of argentine hemorrhagic fever. twenty-six of 29 seropositive animals were the cricetid rodent calomys musculinus, for a 30-month prevalence of 7.9% in that species. combining these data with previously published data on antigen detection provided an estimated total prevalence of infection of 10.9% for this, the pr ... | 1994 | 7985747 |
| prevalence of infection with junin virus in rodent populations in the epidemic area of argentine hemorrhagic fever. | we report the results of indirect fluorescent antibody screening for antibody to junin virus in 1,101 sera from small mammals captured on two mark-recapture grids in the epidemic area of argentine hemorrhagic fever. twenty-six of 29 seropositive animals were the cricetid rodent calomys musculinus, for a 30-month prevalence of 7.9% in that species. combining these data with previously published data on antigen detection provided an estimated total prevalence of infection of 10.9% for this, the pr ... | 1994 | 7985747 |
| latin american hemorrhagic fever. | several viral and bacterial agents are responsible for hemorrhagic fever in latin america, but there are three agents that are only present in this region: (1) the junin virus, (2) the machupo virus, and (3) the guanaritovirus, members of the tacaribe complex of the family arenaviridae and responsible for the argentinian, bolivian, and venezuelan hemorrhagic fever. the clinical manifestations of hemorrhagic fever are similar for the three viruses, mainly myalgia, arthralgia, headache, relative b ... | 1994 | 8021448 |
| the entry of junin virus into vero cells. | the entry mechanism of junin virus (jv) into vero cells was studied analyzing the effect of lysosomotropic compounds and acid ph on jv infection. ammonium chloride, amantadine, chlorpheniramine and procaine inhibited jv production. the action of ammonium chloride was exerted at early times of infection. virus internalization was inhibited and viral protein expression was not detected. when the extracellular medium was buffered at low ph, the ammonium chloride induced block on jv infection was ov ... | 1994 | 8031239 |
| heterogeneity and stability characteristics of candid 1 attenuated strain of junin virus. | fourteen clones of candid 1 strain of junin virus were studied in terms of their attenuation and stability for 9 days-old mice. the cluster analysis of the efficiency of lethal infection, namely the virulence (lethality) indicates (pfu/ld50) of the clones showed that the strain population is heterogeneous. it is constituted by subpopulations with lower and higher virulence as compared to the parent strain. moreover, one or two passages through mice and calomys musculinus, the main junin virus re ... | 1993 | 8105651 |
| heterogeneity and stability characteristics of candid 1 attenuated strain of junin virus. | fourteen clones of candid 1 strain of junin virus were studied in terms of their attenuation and stability for 9 days-old mice. the cluster analysis of the efficiency of lethal infection, namely the virulence (lethality) indicates (pfu/ld50) of the clones showed that the strain population is heterogeneous. it is constituted by subpopulations with lower and higher virulence as compared to the parent strain. moreover, one or two passages through mice and calomys musculinus, the main junin virus re ... | 1993 | 8105651 |
| antiviral treatment of argentine hemorrhagic fever. | argentine hemorrhagic fever is a systemic viral disease caused by junin virus, with a mortality of 15-30% in untreated individuals. current specific therapy is highly effective in reducing mortality, and consists of the early administration of immune plasma in defined doses of specific neutralizing antibodies per kg of body weight. however, several reasons suggest the need to investigate alternative therapies. ribavirin, a broad spectrum antiviral agent, is effective in the treatment of other vi ... | 1994 | 8141590 |
| in vitro selection of junin virus antigenic variants. | the establishment of an experimental persistent infection with junin virus, the aetiological agent of argentine hemorrhagic fever, involves the emergence of antigenic variants in brain and blood of the cricetid calomys musculinus. we demonstrate that antigenic variants can also be isolated in vitro under the selective pressure of polyclonal antibodies and from a long-term infected c. musculinus primary embryo fibroblast culture. the participation of neutralizing antibodies and host cells in the ... | 1993 | 8382041 |
| in vitro selection of junin virus antigenic variants. | the establishment of an experimental persistent infection with junin virus, the aetiological agent of argentine hemorrhagic fever, involves the emergence of antigenic variants in brain and blood of the cricetid calomys musculinus. we demonstrate that antigenic variants can also be isolated in vitro under the selective pressure of polyclonal antibodies and from a long-term infected c. musculinus primary embryo fibroblast culture. the participation of neutralizing antibodies and host cells in the ... | 1993 | 8382041 |
| a simple nucleic acid amplification assay for the rapid detection of junín virus in whole blood samples. | argentine hemorrhagic fever (ahf) is an endemoepidemic disease with cardiovascular, renal and neurologic alterations acquired in the richest farming land in argentina. it is caused by junín virus, one of the few human pathogenic arenaviruses. the s rna of junín virus has been molecularly cloned and its nucleotide sequence determined in our laboratory. this information was used to develop a rapid nucleic acid-based diagnostic test commensurate with the low viraemia detected in ahf patients. junín ... | 1993 | 8383393 |
| safety and immunogenicity of a live-attenuated junin (argentine hemorrhagic fever) vaccine in rhesus macaques. | the safety and immunogenicity of candid #1, a live-attenuated junin virus vaccine, were evaluated in rhesus macaques. candid #1 was inoculated subcutaneously in graded doses ranging from 16 to 127,200 plaque-forming units (pfu) into four groups of five animals each; four controls received saline. there was no significant effect of the immunization on any physical, hematologic, or biochemical parameter measured. junin virus was recovered by cocultivation from peripheral blood mononuclear cells (p ... | 1993 | 8385886 |
| safety and immunogenicity of a live-attenuated junin (argentine hemorrhagic fever) vaccine in rhesus macaques. | the safety and immunogenicity of candid #1, a live-attenuated junin virus vaccine, were evaluated in rhesus macaques. candid #1 was inoculated subcutaneously in graded doses ranging from 16 to 127,200 plaque-forming units (pfu) into four groups of five animals each; four controls received saline. there was no significant effect of the immunization on any physical, hematologic, or biochemical parameter measured. junin virus was recovered by cocultivation from peripheral blood mononuclear cells (p ... | 1993 | 8385886 |
| replication and physical parameters important for preparing purified junin virus. | junin virus (jv) is an arenavirus and the causative agent of argentine hemorrhagic fever (ahf), an often fatal human disease. the attenuated strain xj-clone 3 (xjc13) of jv, after being tested in humans, has been considered a promising vaccine. we found that synthesis of jv xjc13 reaches a peak 2 days after infection and the kinetics of synthesis are little affected by the multiplicity of infection (moi) in a range from 0.125 to 1.00. virus synthesis is sensitive to actinomycin d, indicating tha ... | 1993 | 8388396 |
| the effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of junin virus. | the effects of specific inhibitors of glycoprotein trimming reactions on junin virus (jv) replication were investigated. bromoconduritol, an inhibitor of glucosidase ii, significantly reduced infective virus production (de50: 1.1 mm) and viral protein expression. neither 1-deoxynojirimycin, an inhibitor of both glucosidases i and ii, nor 1-deoxymannojirimycin and swainsonine, inhibitors of mannosidase i and ii, respectively, showed any activity against jv multiplication. these results are the fi ... | 1993 | 8391500 |
| [retrospective detection of hantavirus clinical infections in argentina]. | hantavirus activity in rodents and human beings in argentina has been known since the 1980's. in this study, we retrospectively investigated hantavirus infections among argentine hemorrhagic fever (ahf) cases notified between 1987 and 1994, without virological confirmation. igg and igm antibodies to hantavirus were tested by elisa. among 1028 patients included in the study, we found 13 recent infections (1.26%) and 13 remote infections (1.26%). igg antibodies determined in 745 healthy persons li ... | 1996 | 8734923 |
| low-ph-induced fusion of vero cells infected with junin virus. | junin virus (jv) infected vero cells were used to investigate virus capacity to induce cell-cell fusion. polykaryocyte formation due to jv was found to be ph and temperature-dependent. a reduced fusion activity was detected on bhk-21 cells. different jv-strains exhibited a similar extent and ph dependence of their fusion activity. neutralizing antibodies against the main viral glycoprotein (gp38) inhibited syncytium production and gp38 conformational changes in response to acid treatment were de ... | 1996 | 8774689 |
| the role of mononuclear blood cells in experimental junin virus spread to the central nervous system. | the neuroinvasiveness of junin virus depends on the viral strain, animal species, and age. the role of infected blood cells in hematogenous junin virus spread to the central nervous system (cns) was studied by determining the growth in pheripheral mononuclear cells and brain tissue of candid 1 and xjcl3 laboratory strains, in calomys musculinus and guinea pigs. the present study demonstrated that junin virus replicates in circulating peripheral lymphocytes and macrophages of 11-day-old guinea pi ... | 1995 | 8825295 |
| the role of mononuclear blood cells in experimental junin virus spread to the central nervous system. | the neuroinvasiveness of junin virus depends on the viral strain, animal species, and age. the role of infected blood cells in hematogenous junin virus spread to the central nervous system (cns) was studied by determining the growth in pheripheral mononuclear cells and brain tissue of candid 1 and xjcl3 laboratory strains, in calomys musculinus and guinea pigs. the present study demonstrated that junin virus replicates in circulating peripheral lymphocytes and macrophages of 11-day-old guinea pi ... | 1995 | 8825295 |
| antiviral sulfated steroids from the ophiuroid ophioplocus januarii. | one new and three known sulfated steroidal polyols have been isolated from the ophiuroid ophioplocus januarii, collected at san antonio oeste, río negro, argentina. the four compounds possess 4 alpha,11 beta-dihydroxy-3 alpha,21-disulfoxy substituents and the a/b cis ring junction but differ in the side chain. the new compound has been characterized as (22e)-5 beta-24-norcholest-22-ene-3 alpha,4 alpha,11 beta,21-tetrol 3,21-disulfate (4). the structures of the four compounds were determined from ... | 1996 | 8864241 |
| molecular characteristics of junin virus. | results suggest that protein, glycerophospolipid, galactoside, and sialyl glycoside residues are present in junin virus (jv), are accessible to enzymatic digestion, and play an important role in infection. four major protein bands with molecular masses (mr) 64 +/- 2, 56 +/- 2, 52 +/- 3 (mean +/- standard deviation, n = 4) and approximately 12-18 kda were consistently detected after denaturing gel electrophoresis analysis of purified attenuated jv. the 52 kda protein showed a diffuse tail in the ... | 1997 | 9015273 |
| glial fibrillary acidic protein (gfap) immunochemical profile after junin virus infection of rat cultured astrocytes. | cultured astrocytes derived from newborn rat brain were inoculated with junin virus (jv) to characterize their response to infection by means of their glial fibrillary acidic protein (gfap) immunochemical profile. samples from 1 to 11 days post-inoculation (pi), as well as matched controls, were serially harvested for gfap labeling by peroxidase-antiperoxidase (pap) method. it was only at day 3 that significantly greater values of gfap staining (p < 0.05) were disclosed by three complementary ap ... | 1995 | 9064605 |
| the glycoprotein precursor gene of the attenuated junin virus vaccine strain (candid #1). | a live attenuated virus vaccine has been recently developed to prevent argentine hemorrhagic fever. in this paper, we report the nucleotide sequence of the glycoprotein precursor gene (gpc) of the junin virus vaccine strain (candid #1) and its flanking untranslated regions. the untranslated regions flanking the gpc genes of different arenaviruses are variable in length, sequence, and secondary structure. however, when this highly attenuated junin virus strain is compared with the mc2 strain, whi ... | 1997 | 9080883 |
| molecular characterization of attenuated junin virus strains. | the junin virus strain candid #1 was developed as a live attenuated vaccine for argentine haemorrhagic fever. in this paper we report the nucleotide sequences of s rna of candid #1 and its more virulent ancestors xj#44 and xj (prototype). their relationship to junin virus wild-type mc2 strain and other closely and distantly related arenaviruses was also examined. comparisons of the nucleotide and amino acid sequences of n and gpc genes from candid #1 and its progenitor strains revealed some chan ... | 1997 | 9225036 |
| glycoprotein-mediated biological properties of a host range mutant of junin virus. | the biological properties mediated by the main envelope glycoprotein (gp1) of a mouse-attenuated mutant of junin virus, named cl67, were investigated in comparison with its parental strain xjcl3. in contrast to the parental strain, this mutant was unable to multiply in primary cultures of mouse embryo fibroblasts (mefs). impairment of cl67 multiplication was associated with a lack of virus binding to mef, probably due to an altered interaction between gp1 and cellular receptors. antigenic and im ... | 1997 | 9403931 |
| protective efficacy of a live attenuated vaccine against argentine hemorrhagic fever. ahf study group. | argentine hemorrhagic fever (ahf), caused by the arenavirus junin, is a major public health problem among agricultural workers in argentina. a prospective, randomized, double-blind, placebo-controlled, efficacy trial of candid 1, a live attenuated junin virus vaccine, was conducted over two consecutive epidemic seasons among 6500 male agricultural workers in the ahf-endemic region. twenty-three men developed laboratory-confirmed ahf during the study; 22 received placebo and 1 received vaccine (v ... | 1998 | 9466512 |
| evaluation of an enzyme immunosorbent assay for the diagnosis of argentine haemorrhagic fever. | to elaborate a set of serological tests for the diagnosis of argentine haemorrhagic fever (ahf), an enzyme-linked immunosorbent assay (elisa) for detection of specific anti-junin virus (jv) igg is described, and its performance is compared with that of the plaque reduction neutralization test (prnt). the reproducibility, sensitivity, specificity, and confidence limits for positive and negative results for elisa were statistically analysed. the value of 800 was demonstrated as the lowest positive ... | 1997 | 9607087 |
| in vitro anti-junin virus activity of a peptide isolated from melia azedarach l. leaves. | meliacine, a peptide isolated from leaves of melia azedarach l. inhibited the multiplication of junin virus in vero cells treated with the compound before infection (pre-treatment) or immediately after virus adsorption. analysis of early events following infection demonstrated that meliacine blocks virus penetration by preventing the uncoating step. the addition of meliacine at different times after infection indicated that meliacine also interferes with the release of infectious particles to th ... | 1998 | 9624546 |
| [prevalence of hepatitis c antibodies in plasma donors for the treatment of argentine hemorrhagic fever]. | for argentine hemorrhagic fever, a disease caused by junin virus (jv), there is an effective treatment, consisting of the transfusion of immune plasma (ip). this plasma is obtained from individuals who have had the disease. since hepatitis c virus (hcv) is transmitted parenterally, this study was aimed to estimate the prevalence of anti-hcv in a population of ip donors. in this study, 376 donors (47 females and 329 males) were studied: 95 individuals (24 females and 71 males) who had had fha but ... | 1997 | 9640761 |
| effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of junin virus. | the effect of glycoprotein processing and transport on junin virus (jv) maturation was studied by using brefeldin a (bfa) and carbonyl cyanide m-chlorophenylhydrazone (cccp), two inhibitors affecting different steps of the intracellular exocytic pathway, combined with low temperature incubation. both compounds inhibited the multiplication of jv, strain iv4454, in vero cells in a dose dependent manner. the addition of the compounds after several cycles of infection for a very short treatment peri ... | 1997 | 9672585 |