Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| cloning and inactivation of the gene responsible for a major surface antigen on streptococcus mutans. | to understand more fully the biological function(s) and investigate the reported cross-reactivity with heart tissue of antigen p1 (i/ii) of streptococcus mutans (serotype c), this molecular biological study of the responsible gene, spap, was undertaken. a 5.2 kb hin diii fragment of strain ng5 was cloned into escherichia coli jm109 by a shotgun procedure with puc18 as the vector. recombinant sm2949 expressed a p1 fusion protein under the control of the streptococcal promoter. southern analysis r ... | 1990 | 2088219 |
| molecular cloning and expression of antigens from actinobacillus actinomycetemcomitans in escherichia coli. | in order to study the role of membrane proteins in mucosal colonization by actinobacillus actinomycetemcomitans, a plasmid library was constructed by ligating ecori-digested genomic dna from a. actinomycetemcomitans strain y4 into ecori-digested and dephosphorylated puc13 dna. a second library was constructed by ligating sau3a-digested and size-fractionated a. actinomycetemcomitans strain y4 dna into bamhi-cleaved and dephosphorylated puc13 dna. the dna was transformed into escherichia coli stra ... | 1990 | 2088237 |
| skeletal muscle po2 during hypodynamic sepsis. | 1990 | 2096660 | |
| thermostable beta-galactosidase from the archaebacterium sulfolobus solfataricus. purification and properties. | a thermophilic and thermostable beta-galactosidase activity was purified to homogeneity from crude extracts of the archaebacterium sulfolobus solfataricus, by a procedure including ion-exchange and affinity chromatography. the homogeneous enzyme had a specific activity of 116.4 units/mg at 75 degrees c with o-nitrophenyl beta-galactopyranoside as substrate. molecular mass studies demonstrated that the s. solfataricus beta-galactosidase was a tetramer of 240 +/- 8 kda composed of similar or ident ... | 1990 | 2105216 |
| synthesis and characterization of escherichia coli o18 o-polysaccharide conjugate vaccines. | nontoxic, serologically reactive o polysaccharide was derived from escherichia coli o18 lipopolysaccharide by acid hydrolysis, extraction with organic solvents, and gel filtration chromatography. oxidized o polysaccharide was covalently coupled to either pseudomonas aeruginosa toxin a or cholera toxin by using adipic acid dihydrazide as a spacer molecule in the presence of carbodiimide. the resulting conjugates were composed of approximately equal amounts of o polysaccharide and protein and were ... | 1990 | 2105272 |
| a recombinant rickettsia conorii vaccine protects guinea pigs from experimental boutonneuse fever and rocky mountain spotted fever. | there are no vaccines against boutonneuse fever and rocky mountain spotted fever. previous studies have identified a rickettsia rickettsii surface protein as a vaccine candidate and shown that an antigenically related protein is present in r. conorii, which causes boutonneuse fever. the gene encoding the r. rickettsii protein has been cloned and expressed in escherichia coli. we confirmed by 7.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis of rickettsial lysates followed by immunob ... | 1990 | 2106490 |
| fever response induced by intravenous and intracerebroventricular injection of pyrogen in thyroidectomised and protein-calorie malnourished rabbits. | the development of a fever in response to intravenous (iv, 1.5 micrograms/kg body mass) and intracerebroventricular (icv, 1.5 micrograms/animal) injections of escherichia coli lipopolysaccharide (lps) was studied in control, thyroidectomised and protein-calorie malnourished rabbits (new zealand whites, n = 55). icv injection of lps in control rabbits produced a fever response, the characteristics of which differed from those obtained after iv pyrogen injection. thyroid deficiency caused an atten ... | 1990 | 2107523 |
| differential binding of p. aeruginosa and s. aureus to corneal epithelium in culture. | adherence of bacteria to corneal epithelium is a prerequisite for corneal infection. we used two methods to study the binding of pseudomonas aeruginosa and staphylococcus aureus to rabbit corneal epithelial cells in culture. in the first method, rabbit corneal epithelial cells grown on glass slides were incubated with p. aeruginosa or s. aureus (10(7) cfu/ml) at room temperature for 90 min, and the bacterial binding to the epithelial cells was examined by light microscopy. both p. aeruginosa and ... | 1990 | 2110556 |
| eukaryotic coupled translation of tandem cistrons: identification of the influenza b virus bm2 polypeptide. | previous nucleotide sequence analysis of rna segment 7 of influenza b virus indicated that, in addition to the reading frame encoding the 248 amino acid m1 protein, there is a second overlapping reading frame (bm2orf) of 585 nucleotides that has the coding capacity for 195 amino acids. to search for a polypeptide product derived from bm2orf, a genetically engineered beta-galactosidase-bm2orf fusion protein was expressed in escherichia coli and a polyclonal rabbit antiserum was raised to the puri ... | 1990 | 2114979 |
| properties of a novel plasminogen activator (bm 06.022) produced in escherichia coli. | since currently available thrombolytics still show disadvantages, such as administration by infusion, occurrence of intracranial hemorrhage, major hemorrhagic complications, allergic reactions, and high price, a novel tissue plasminogen activator has been developed. bm 06.022 is a t-pa mutant produced in escherichia coli by dna technology. it has no oligosaccharide side-chains and comprises the kringle 2- and protease domains of t-pa. like t-pa, the enzymatic activity of bm 06.022 can be stimula ... | 1990 | 2129142 |
| radio-immunoassay for formyl methionyl leucyl phenylalanine. i. development and application to assessment of chemotactic peptide production by enteric bacteria. | bacterial chemotactic peptides are low molecular weight peptides which stimulate a wide range of neutrophil functions following binding to specific leucocyte receptors. formyl methionyl leucyl phenylalanine (fmlp) is the major chemotactic peptide in escherichia coli culture supernatants. this paper reports the development and validation of a radio-immunoassay (ria) for fmlp and its application to the analysis of formyl peptide production by enteric bacteria in vitro. the assay was moderately sen ... | 1990 | 2129421 |
| construction, expression and unexpected regulatory properties of a tropomyosin mutant with a 31-residue deletion at the c-terminus (exon 9). | the cdna coding for human skeletal muscle beta-tropomyosin was expressed in escherichia coli to produce an unacetylated beta-tropomyosin. this cdna was deleted from the sequence corresponding to the exon 9 and expressed in e. coli to produce an unacetylated beta-tropomyosin mutant lacking the c-terminal residues 254-284. the main structural and functional properties of the two isolated proteins, designated tropomyosin-1 and des-(254-284)-tropomyosin, respectively, were characterized in compariso ... | 1990 | 2148519 |
| time course of rabbit ocular inflammatory response and mediator release after intravitreal endotoxin. | an inflammatory response was elicited in the rabbit eye by intravitreal injection of endotoxin. the appearance in aqueous humor of selected metabolites of arachidonic acid metabolism at various times was correlated with the influx of protein and myeloperoxidase activity in the iris-ciliary body. after intravitreal injection of endotoxin, aqueous humor protein levels increased substantially within 2 hr. this aqueous humor protein increase occurred before a significant appearance of prostaglandin ... | 1990 | 2154416 |
| identification and dna sequence of the shope fibroma virus dna topoisomerase gene. | the shope fibroma virus (sfv) dna topoisomerase gene has been identified and mapped to the bamhi d fragment near the midpoint of the genome. the dna sequence of the sfv bamhi s fragment together with the contiguous bamhi-clai subfragment of bamhi d which encompasses the topoisomerase gene and two flanking genes has been determined and analyzed. both the sfv dna topoisomerase and the two flanking genes are closely related in terms of sequence and spatial organization to the homologous sequences f ... | 1990 | 2161144 |
| evaluation of piperacillin-tazobactam in experimental meningitis caused by a beta-lactamase-producing strain of k1-positive escherichia coli. | we evaluated the pharmacokinetics and therapeutic efficacy of piperacillin combined with tazobactam, a novel beta-lactamase inhibitor, in experimental meningitis due to a beta-lactamase-producing strain of k1-positive escherichia coli. different doses of piperacillin and tazobactam, as single agents and combined (8:1 ratio; dosage range, 40/5 to 200/25 mg/kg per h), and of ceftriaxone were given to experimentally infected rabbits by intravenous bolus injection followed by a 5-h constant infusion ... | 1990 | 2163241 |
| yersinia pestis ph 6 antigen: genetic, biochemical, and virulence characterization of a protein involved in the pathogenesis of bubonic plague. | we studied a protein antigen, designated ph 6 ag, that has the same regulation of expression as the previously described yersinia pestis ph 6 ag. monospecific antiserum to this antigen recognized several proteins, ranging from 15 to over 75 kilodaltons (kda), which were strongly expressed when y. pestis was cultivated at 37 degrees c and ph 6 but were expressed weakly, if at all, at 37 degrees c and ph 8 and at 26 degrees c. the antigen appeared to be composed of aggregates of a 15-kda subunit. ... | 1990 | 2164509 |
| species-specific substrate interaction of picornavirus 3c proteinase suballelic exchange mutants. | the substrate recognition properties of the polio-virus type 1 and coxsackievirus b3 3c proteinases have been examined in vitro by allelic and suballelic exchange of 3c between the cloned virus genomes. the activity of the altered 3c proteinases was examined by translation of synthetic rna in a rabbit reticulocyte lysate/hela cell extract translation system. analysis of the subsequent processing of virus polyproteins by the altered 3c proteinases showed that all of the mutant proteinases maintai ... | 1990 | 2168426 |
| specific enzyme-linked immunosorbent assay for the detection of antibodies to the human spumavirus. | recombinant plasmid clones were constructed harbouring the central domains of the outer membrane protein and the transmembrane protein of the env gene of human spumaretrovirus (hsrv). the corresponding fusion proteins were expressed in e. coli, purified and used subsequently to produce antibodies against the hsrv env proteins in rabbits. the authenticity of the bacterially produced domain of the hsrv env proteins was shown by radioimmunoprecipitation of the viral env glycoprotein from hsrv-infec ... | 1990 | 2170434 |
| proton-linked sugar transport systems in bacteria. | the cell membranes of various bacteria contain proton-linked transport systems for d-xylose, l-arabinose, d-galactose, d-glucose, l-rhamnose, l-fucose, lactose, and melibiose. the melibiose transporter of e. coli is linked to both na+ and h+ translocation. the substrate and inhibitor specificities of the monosaccharide transporters are described. by locating, cloning, and sequencing the genes encoding the sugar/h+ transporters in e. coli, the primary sequences of the transport proteins have been ... | 1990 | 2172229 |
| a genetic locus of enteropathogenic escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. | the ability of enteropathogenic escherichia coli (epec) to form attaching and effacing intestinal lesions is a major characteristic of epec pathogenesis. using tnphoa mutagenesis we have identified a chromosomal gene (eae, for e. coli attaching and effacing) that is necessary for this activity. a dna probe derived from this gene hybridizes to 100% of e. coli of epec serogroups that demonstrate attaching and effacing activity on tissue culture cells as well as other pathogenic e. coli that produc ... | 1990 | 2172966 |
| separation of sublethal and lethal effects of the bactericidal/permeability increasing protein on escherichia coli. | binding of the bactericidal/permeability increasing protein (bpi) of granulocytes to escherichia coli promptly produces several discrete outer envelope alterations and growth arrest without major impairment of bacterial structure or biosynthetic capabilities, raising the question whether these early effects of bpi are sufficient to cause bacterial death. in this study, the bactericidal action of bpi was examined more closely. we have found that bovine or human serum albumin blocks bacterial kill ... | 1990 | 2179269 |
| acute endotoxin-induced lymphocyte subset sequestration in sheep lungs. | endotoxemia is associated with an early phase of pulmonary hypertension and a later increase in microvascular permeability. these physiologic changes are attended by peripheral blood and lung lymph leukopenia and a rapid accumulation of both granulocytes and lymphocytes in the peripheral lung. in the present study, the numbers of lymphocytes in blood, lung lymph, and lung tissue after infusion of endotoxin were determined by fluorescent labeling of lymphocyte populations with monoclonal antibodi ... | 1990 | 2179624 |
| the major surface glycoprotein (gp63) is present in both life stages of leishmania. | leishmania exist as extracellular promastigotes which multiply in the gut of the sandfly insect vector and as intracellular amastigotes which divide in the phagolysosome of mononuclear phagocytic cells of the mammalian host. promastigotes express a major surface glycoprotein of 63 kda, referred to as gp63. the expression of gp63 in both leishmania life stages was studied using rabbit antibodies against native gp63 as well as rabbit antibodies against recombinant gp63 that was synthesized in an e ... | 1990 | 2181303 |
| oxygen-derived free radicals mediate the cutaneous necrotizing vasculitis induced by epinephrine in endotoxin-primed rabbits. | to assess the possible role of oxygen-derived free radical in the pathogenesis of cutaneous necrotizing vasculitis (thomas reaction) induced by epinephrine in endotoxin-primed rabbits, animals were treated with superoxide dismutase (intraperitoneal or local), catalase, allopurinol, or ibuprofen 2 h before inducing the reaction. this is done by an earlobe intravenous injection (10 micrograms) of endotoxin followed by an intradermal injection (100 micrograms) of epinephrine in the shaved abdomen; ... | 1990 | 2182724 |
| in vitro antibacterial effect of yogurt on escherichia coli. | we investigated the bactericidal and bacteriostatic effects of yogurt on three strains of escherichia coli: human toxigenic (078:h11), rabbit pathogenic (rdec-1) and rabbit nonpathogenic [015:k14(l):h4]. approximately 10(6) organisms were incubated in yogurt, milk, broth, and modifications of these materials. aliquots were removed at various intervals and plated on macconkey's agar for enumeration of e. coli. yogurt was bactericidal (at least 5 log10 reduction in bacterial counts) to all three s ... | 1990 | 2185003 |
| cloning and sequencing of mammalian glutathione reductase cdna. | the molecular cloning of a partial cdna to mouse glutathione reductase mrna and of a full-length cdna to the mrna of the human enzyme is described. an initial cdna clone designated lambda grm-b11 was isolated by plaque-screening of an induced mouse cdna expression library in the lambda gt11 vector with a rabbit antibody probe to human glutathione reductase. 125iodine-labelled whole anti-rabbit immunoglobulin was used as second antibody. ecori digestion of the lambda grm-b11 clone released a 720- ... | 1990 | 2185014 |
| evidence for two types of cytotoxic necrotizing factor in human and animal clinical isolates of escherichia coli. | we have characterized the in vitro and in vivo toxic properties of cell sonic extracts from 22 animal and human clinical isolates of escherichia coli that caused both necrosis in the rabbit skin and multinucleation in tissue cultures, two toxic properties previously reported as being specific for e. coli cytotoxic necrotizing factor (cnf). two distinct toxic phenotypes were observed. type 1, which was displayed by originally described cnf strains, was characterized by extensive multinucleation a ... | 1990 | 2185259 |
| cloning and expression of a mammalian peptide chain release factor with sequence similarity to tryptophanyl-trna synthetases. | the termination of protein synthesis is encoded by in-frame nonsense (stop) codons. most organisms use three nonsense codons: uga, uag, and uaa. in contrast to sense codons, which are decoded by specific trnas, nonsense codons are decoded by proteins called release factors (rfs). here we report the cloning of a mammalian rf cdna by the use of monoclonal antibodies specific for rabbit rf. functional studies showed that, when expressed in escherichia coli, the protein encoded by this cdna has in v ... | 1990 | 2185472 |
| activity of sulbactam in combination with ceftriaxone in vitro and in experimental endocarditis caused by escherichia coli producing shv-2-like beta-lactamase. | we studied the efficacy of sulbactam, a beta-lactamase inhibitor, in combination with ceftriaxone in vitro and in experimental endocarditis due to an escherichia coli strain producing an extended-spectrum beta-lactamase most similar to shv-2, a new mechanism of resistance to broad-spectrum cephalosporins among members of the family enterobacteriaceae. in vitro, ceftriaxone demonstrated an important inoculum effect (mics were 2 and 256 micrograms/ml with 5 x 10(5) and 5 x 10(7) cfu of inoculum pe ... | 1990 | 2188586 |
| complementation of an escherichia coli proc mutation by a gene cloned from treponema pallidum. | little is known concerning the biosynthetic and metabolic capabilities of the syphilis agent, treponema pallidum, because of the inability to cultivate continuously the organism in vitro. to circumvent the problem of cultivation, researchers have used recombinant dna technology to express treponemal protein antigens in escherichia coli. however, with a few notable exceptions, the specific cellular roles of these cloned treponemal proteins have not been determined. in this study, a cosmid library ... | 1990 | 2188947 |
| escherichia coli and klebsiella vaccines and immunotherapy. | a polyvalent vaccine has been prepared from the capsular polysaccharide of 24 different serotypes of klebsiella spp. nearly 200 volunteers have received this vaccine. it is very well tolerated and elicits both binding (elisa) and functional antibody to 21 of 24 antigens. antibodies were also detected against 10 serotypes not included in the vaccine. an immunoglobulin for intravenous use (ivig) was more protective in mouse lethality assays and enhanced opsonophagocytic killing of bacteria more th ... | 1990 | 2189000 |
| bacterial phospholipid hydrolysis enhances the destruction of escherichia coli ingested by rabbit neutrophils. role of cellular and extracellular phospholipases. | escherichia coli ingested by pmn are promptly growth arrested but undergo limited destruction. we have studied bacterial phospholipid hydrolysis as a possible limiting factor in the disassembly of ingested e. coli, comparing the fates, during phagocytosis by rabbit peritoneal exudate pmn, of three isogenic strains, differing in their content of the plda gene encoding the principal e. coli phospholipase a (pla), i.e., plda-, plda+, plda (the latter strain bearing the plda gene in a multicopy plas ... | 1990 | 2189895 |
| construction of a bivalent oral vaccine for prevention of typhoid fever and cholera diarrhea. | a recombinant plasmid pmm-ctb containing the gene for production of the nontoxic b subunit of vibrio cholera was transferred into a safe, effective and attenuated oral vaccine ty21a strain of salmonella typhi. the resulting ty21a (pmm-ctb) could steadily produce ct-b subunit that was secreted extracellularly and had the same antigenicity as ct-b produced by v. cholera. furthermore, the characteristics of the antigenicity, the persistance in mice and the galactose sensitivity possessed in the str ... | 1990 | 2190570 |
| molecular cloning of a cdna for rat hepatic glutaminase. sequence similarity to kidney-type glutaminase. | mammalian liver possesses a unique isozyme of phosphate-activated glutaminase which plays an important role in the regulation of glutamine catabolism. antibodies to hepatic glutaminase were used to screen a lambda gt11 rat liver cdna library. one cdna to hepatic glutaminase was identified. changes in the relative abundance of hepatic glutaminase mrna were determined by hybridization to this cdna. the mrna is found only in liver; it is not present prior to birth but its abundance increases dramat ... | 1990 | 2191954 |
| cloning, expression, and occurrence of the brucella cu-zn superoxide dismutase. | recently, the complete amino acid sequence of a protein expressed in escherichia coli from cloned brucella abortus dna was reported. on the basis of amino acid homology, this protein was identified as a copper-zinc superoxide dismutase (cu-zn sod) (b. l. beck, l. b. tabatabai, and j. e. mayfield, biochemistry 29:372-376, 1990). we demonstrate in this paper that the sequenced protein is the same as the previously studied salt-extractable protein bcsp20. the plasmid-encoded protein expressed from ... | 1990 | 2201639 |
| biological properties of lipopolysaccharides from bordetella species. | biological activities of lipopolysaccharides (lps) extracted from bordetella pertussis, b. parapertussis and b. bronchiseptica were compared with those of escherichia coli lps. the lps preparations from b. pertussis showed biological activities comparable to those of e. coli lps in terms of lethal toxicity in galactosamine-sensitized mice, pyrogenicity in rabbits, mitogenicity in c3h/he spleen cell cultures, macrophage activation, and induction of tumour necrosis factor. all the activities of lp ... | 1990 | 2202781 |
| cloning and characterization of rna polymerase core subunits of chlamydia trachomatis by using the polymerase chain reaction. | taking advantage of sequence conservation of portions of the alpha, beta, and beta' subunits of rna polymerase of bacteria and plant chloroplasts, we have designed degenerate oligonucleotides corresponding to these domains and used these synthetic dna sequences as primers in a polymerase chain reaction to amplify dna sequences from the chlamydial genome. the polymerase chain reaction products were used as a probe to recover the genomic fragments encoding the beta subunit and the 5' portion of th ... | 1990 | 2211507 |
| insertional inactivation of the gene encoding a 76-kilodalton cell surface polypeptide in streptococcus gordonii challis has a pleiotropic effect on cell surface composition and properties. | a library of streptococcus gordonii dl1-challis dna was constructed in lambda gt11. phage plaques were screened for production of antigens that reacted with antiserum to s. gordonii cell surface proteins. a recombinant phage denoted lambda gt11-cp2 was isolated that carried 1.85 kb of s. gordonii dna and that expressed an antigen with a molecular mass of 29 kda in escherichia coli. antibodies that reacted with the expression product were affinity purified and were shown to react with a single po ... | 1990 | 2228239 |
| presequence does not prevent folding of a purified mitochondrial precursor protein and is essential for association with a reticulocyte cytosolic factor(s). | ornithine carbamoyltransferase (otc; subunit, 36,000 da) [ec 2.1.3.3] is initially synthesized as a precursor (potc) with a transient nh2-terminal presequence of 32 amino acid residues, then is imported posttranslationally nto the mitochondrial matrix. we expressed rat potc in escherichia coli, purified it in a denatured form, and showed that could be transported into isolated mitochondria in the presence of rabbit reticulocyte lysate [murakami et al. (1988) j. biol. chem. 263, 18437-18442]. in ... | 1990 | 2229023 |
| evidence for in vivo synthesis of thiamin triphosphate by cytosolic adenylate kinase in chicken skeletal muscle. | we showed previously that cytosolic adenylate kinase (ak1) purified from pig skeletal muscle catalyzes in vitro formation of thiamin triphosphate (ttp) from thiamin diphosphate (tdp) and adp in addition to atp formation from adp [shikata, h. et al. (1989) biochem. int. 18, 933-942]. to obtain evidence for in vivo synthesis of ttp by ak1, changes in ttp content and ak1 activity were determined in chicken skeletal muscle during development after hatching. thiamin phosphate metabolism in chicken sk ... | 1990 | 2229026 |
| embryonic neural cell adhesion molecule in cerebrospinal fluid of younger children: age-dependent decrease during the first year. | poly-alpha-2,8-n-acetylneuraminic acid (poly-alpha-2,8-neuac) is developmentally expressed in neural tissue of higher animals, where it is covalently attached to the neural cell adhesion molecule (ncam), a large integral membrane glycoprotein mediating cell-cell adhesion during neuronal development. ncam exists in several molecular forms, of which only embryonic ncam carries lengthy chains (n greater than 5) of poly-alpha-2,8-neuac. chemically identical poly-alpha-2,8-neuac of bacterial origin i ... | 1990 | 2230809 |
| parental influenza virion nucleocapsids are efficiently transported into the nuclei of murine cells expressing the nuclear interferon-induced mx protein. | the interferon-induced murine mx1 protein, which is localized in the nucleus, most likely specifically blocks influenza virus replication by inhibiting nuclear viral mrna synthesis, including the mrna synthesis catalyzed by inoculum (parental) virion nucleocapsids (r. m. krug, m. shaw, b. broni, g. shapiro, and o. haller, j. virol. 56:201-206, 1985). we tested two possible mechanisms for this inhibition. first, we determined whether the transport of parental nucleocapsids into the nucleus was in ... | 1990 | 2243397 |
| in vitro cytotoxic effect of alpha-hemolytic escherichia coli on human blood granulocytes. inhibition by alpha-hemolysin antibody. | the influence of alpha-hemolysin antibody on the in vitro cytotoxic effect of alpha-hemolytic escherichia coli bacteria and culture filtrates was investigated. damage to human blood granulocytes was quantified by measuring the release of chromium 51 from labelled cells in the presence of whole or fractionated plasma containing alpha-hemolysin antibody. anti-alpha-hemolysin activity was found exclusively in the igg fraction of plasma. human plasma contained "natural" alpha-hemolysin antibody to v ... | 1990 | 2248684 |
| high-level production of escherichia coli stb heat-stable enterotoxin and quantification by a direct enzyme-linked immunosorbent assay. | a convenient and sensitive enzyme-linked immunosorbent assay (elisa) for the stb heat-stable enterotoxin of escherichia coli was developed and used to quantify stb production by strains with a high level of expression. based on an antigenic profile of the secreted form of stb, a synthetic peptide (stb3-27) spanning the major predicted epitope was synthesized, coupled to keyhole limpet hemocyanin, and used to immunize rabbits. anti-stb3-27 antibodies were affinity purified on a synthetic peptide- ... | 1990 | 2254413 |
| endotoxin requirements for alveolar macrophage stimulation. | acute pulmonary failure or ards in severely injured patients continues to be a significant problem. the most important clinical risk factor identified is sepsis syndrome. sepsis syndrome is the clinical correlate of a malignant systemic inflammatory process and is directed in large part by the tissue-fixed macrophage (m phi), such as the alveolar m phi. the m phi is capable of producing most of the central inflammatory mediators responsible for the pathophysiology seen during sepsis and organ in ... | 1990 | 2254991 |
| a single amino acid substitution in the a subunit of escherichia coli enterotoxin results in a loss of its toxic activity. | a plasmid encoding a mutant gene of heat-labile enterotoxin (lt), produced by enterotoxigenic escherichia coli, was induced by treatment of plasmid ewd 299 with hydroxylamine. a mutant strain of e. coli hb 101 carrying the mutant plasmid ptuh 6a produced a low toxic lt analogue (mutant lt), which was cross-reactive with anti-lt antibody. the mutant lt activity was less than 0.15 and 0.006% of the normal lt in the rabbit ileal loop test and in the rabbit skin permeability test, respectively. the ... | 1990 | 2266142 |
| translation of preprochymosin in vitro. evidence for folding of prochymosin to the native conformation. | 1. the cdna coding for preprochymosin has been sub-cloned into the transcription/translation vector pgem-3z, the t7 promoter used to transcribe the gene and the product expressed in an 'in vitro' cell-free system comprising rabbit reticulocyte lysate and dog pancreatic microsomes. 2. translations in various conditions, and analyses of the translation product in reducing and non-reducing conditions, indicate that oxidizing translation conditions and the cleavage of the n-terminal 'pre-' sequence ... | 1990 | 2268293 |
| identification of cbs2 as a mitochondrial protein in saccharomyces cerevisiae. | the nuclear genome encoded yeast protein cbs2 is required for translational activation of mitochondrial cytochrome b rna. genetic studies have shown that the target sequence of the cbs2 protein is the 5' untranslated leader sequence of cytochrome b rna. here we report on the intracellular localization of cbs2. cbs2 protein, expressed in escherichia coli and prepared from inclusion bodies, was used as an antigen to raise a polyclonal rabbit antiserum. affinity-purified cbs2 antibodies detect a 45 ... | 1990 | 2270079 |
| [antibiotic and specific immunoglobulin g in the treatment of e. coli sepsis in mice previously given steroids]. | experimental e. coli sepsis was constituted in two groups of mice. one of the groups have been immunosuppressed by steroid therapy of ten days (group a) and the other group was normal (group b). ceftriaxone and ig g, prepared by vaccinating rabbits with the same strain of e. coli, were used in therapy. the results of the therapy in both were compared. the mortality rate of mice on ig g therapy was % 70 in group a and % 80 in group b and that on ceftriaxone therapy % 60 and % 50, respectively. th ... | 1990 | 2287291 |
| production of specific monoclonal antibodies to salmonella typhi flagellin and possible application to immunodiagnosis of typhoid fever. | four murine monoclonal antibodies (mabs) to salmonella typhi flagellin were produced. these mabs did not react with eight other enterobacterial strains tested: salmonella enteritidis, salmonella typhimurium, salmonella paratyphi a, escherichia coli, shigella flexneri, shigella sonnei, yersinia enterocolitica, and campylobacter jejuni. all four mabs cross-reacted with salmonella muenchen flagellin indicating specificity for d antigenic flagellar epitope. one mab (c4) was selected to develop a dou ... | 1990 | 2295860 |
| high level bacterial expression of uteroglobin, a dimeric eukaryotic protein with two interchain disulfide bridges, in its natural quaternary structure. | bacterial expression of eukaryotic proteins is a tool of ever-increasing importance in biochemistry and molecular biology. however, the majority of the recombinant eukaryotic proteins that have been expressed in bacteria are produced as fusion proteins and not in their native conformation. in particular, correct formation of quaternary structures by recombinant proteins in bacterial hosts has been reported very rarely. to our knowledge, correct intracellular formation of multimeric structures co ... | 1990 | 2318861 |
| the gene sequence and some properties of protein h. a novel igg-binding protein. | the gene for protein h, a novel bacterial cell wall protein with specific affinity for human igg fc, was cloned from a group a streptococcus and expressed in escherichia coli. recombinant e. coli cells produced two forms of a human igg fc-binding protein, one with an apparent mr of 42 kda in a periplasmic fraction and the other with an apparent mr of 45 kda in a mixed fraction of cytoplasms and membranes. both 42-kda and 45-kda protein preparations similarly bound to human igg1 to igg4, human ig ... | 1990 | 2332638 |
| effects of endotoxin and anti-inflammatory agents on superoxide dismutase in the rabbit iris. | superoxide dismutase activity (sod) was measured in the irides of control animals and 24 h after the intravitreal administration of escherichia coli endotoxin. a 3-fold increase in sod was noted (p less than 0.001). to protect against the inflammatory process, either topical dexamethasone (dex) or medrysone (med) was administered t.i.d. for 2 days before and 1 day after endotoxin. although dex prevented the iritis seen with endotoxin alone, a 2-fold increase in sod was measured (p less than 0.00 ... | 1990 | 2342774 |
| ocular inflammatory effects of intravitreally injected tumor necrosis factor-alpha and endotoxin. | intravitreal injection of human recombinant tumor necrosis factor-alpha (tnf) induced inflammation in the rabbit eye characterized by dilation of blood vessels in the iris, disruption of the blood-ocular barriers, infiltration of inflammatory cells into the anterior chamber, and accumulation of prostaglandin e in intraocular fluids. inflammation first appeared on day 1, increased on day 2, and remained elevated on day 7. the inflammatory cell infiltrate in the anterior segment of the eye was lar ... | 1990 | 2361736 |
| the role of modified purine 64 in initiator/elongator discrimination of trna(imet) from yeast and wheat germ. | the role of 2'-ribosylated adenosine 64 in trna(imet) from yeast in initiation/elongation discrimination was investigated. as measured by in vitro translation in rabbit reticulocyte lysate, the specific removal of the 2'-ribosylphosphate at adenosine 64 via periodate oxidation allows trna(imet) to read internal aug codons of the globine messenger rna. yeast met-trna(imet) lacking the modification of nucleoside 64 forms ternary complexes with gtp and elongation factor tu from escherichia coli. th ... | 1990 | 2395634 |
| schistosoma mansoni: cloning of antigen gene sequences in escherichia coli. | fischer rat protective antiserum (f-2x) prepared from schistosoma mansoni-infected rats was used to screen an adult worm cdna library constructed in a lambda gt11 bacteriophage expression vector. this led to the isolation of several clones yielding proteins reactive with antibodies in the infection serum. counter-screening of these clones with wistar-furth rat nonprotective antiserum (w-2x) enabled identification of clones either uniquely or preferentially reacting with f-2x, in addition to clon ... | 1990 | 2403933 |
| deletion of carboxy-terminal residues of murine granulocyte-macrophage colony-stimulating factor results in a loss of biologic activity and altered glycosylation. | a deletion mutant of murine granulocyte-macrophage colony-stimulating factor (gm-csf) which differs in primary structure from native gm-csf in the carboxy-terminal 11 amino acids was prepared. four amino acid residues are mutated and the seven terminal residues including cys-118 are deleted. supernatants from cos-1 cells transfected with this deletion mutant (gm-csf(del] showed a 3000-fold decrease in the ability to stimulate bone marrow stem cells to proliferate and differentiate into granulocy ... | 1990 | 2404451 |
| specificity and inhibitory activity of antibodies to plasmodium falciparum aldolase. | the multiplication of plasmodium falciparum within rbc is energy-dependent and the glucose consumption of infected rbc is increased more than 50 times over the consumption of normal rbc. high levels of glycolytic enzymes such as fructose-1,6-diphosphate aldolase (p41) have been detected in infected rbc. expression of the cloned aldolase gene of p. falciparum in escherichia coli resulted in an enzymatically active polypeptide with a high sp. act. and the recombinant p41 aldolase was used for enzy ... | 1990 | 2406342 |
| membrane morphogenesis from cloned fragments of bacteriophage pm2 dna that contain the sp6.6 gene. | the formation of new membrane vesicles normally occurs during eukaryotic organellogenesis and maturation of bacteriophage pm2. this virus was studied as a simple model for membrane morphogenesis. previous biochemical and genetic studies suggest that a major structural protein of pm2, sp6.6, is an integral membrane protein involved in viral membrane morphogenesis. to establish the necessity of sp6.6 in membrane formation, restriction fragments of pm2 that contained the sp6.6 coding sequence were ... | 1990 | 2407591 |
| precise epitope mapping of the murine transformation-associated protein, p53. | murine p53 cdna sequences were cloned into an in vitro expression vector, protem hind. four deletion libraries were generated using bal31 double-stranded exonuclease; two being made from constructs encoding a fusion protein constructed from sv40 small t sequences and the p53 clone, p27.la; and two from the full length p53 clone, pp53-5. both 5'- and 3'-terminal deletions of the p53 gene were made. transcription of these constructs using escherichia coli rna polymerase holoenzyme, followed by tra ... | 1985 | 2408882 |
| major antigenic determinants of f and colb2 pili. | f-like conjugative pili are expressed by plasmids with closely related transfer systems. they are tubular filaments that are composed of repeating pilin subunits arranged in a helical array. both f and colb2 pilin have nearly identical protein sequences, and both contain an acetylated amino-terminal alanine residue. however, they differ by a few amino acid residues at their amino termini. rabbit antisera raised against purified f and colb2 pili are immunologically cross-reactive by only 25%, as ... | 1985 | 2409073 |
| deposition of c3b and ic3b onto particulate activators of the human complement system. quantitation with monoclonal antibodies to human c3. | monoclonal antibodies were used to determine the number and molecular form of c3 bound to particulate activators of the complement (c) system by human serum. sheep erythrocytes (e) coated with igm (eigm) and igg (eigg) were used to study activation of the classical pathway (cp). yeast (y), rabbit erythrocytes (er), and five species of bacteria (escherichia coli, staphylococcus aureus, streptococcus pneumoniae type 3, streptococcus pyogenes, and hemophilus influenzae type b) were used to study ac ... | 1985 | 2409200 |
| the accessibility of 5-methylcytosine to specific antibodies in double-stranded dna of xanthomonas phage xp12. | antibodies specifically directed to 5-methylcytidine were raised in rabbits and purified by affinity chromatography. the accessibility of 5-methyldeoxycytidine (m5dcyd) to such antibodies was studied with dnas from various origins. the reaction was followed by measuring the retention of radiolabelled dna by antibodies on nitrocellulose filters, by immunoprecipitation, by gel filtration and was visualized with the electron microscope. antibodies did not bind to escherichia coli b dna, which is de ... | 1985 | 2412815 |
| shwartzman reaction in germfree rabbits. | the local shwartzman reaction occurred in germfree rabbits which had no natural antibody to endotoxin and none or only a very small amount of immunoglobulin g. from the results it was concluded that the presence of natural antibody to endotoxin is not a prerequisite of the production of the local shwartzman reaction by bacterial endotoxin. | 1985 | 2412964 |
| cross-reactivity of rabbit antibodies to lipopolysaccharides of escherichia coli j5 and other gram-negative bacteria. | antiserum to rough gram-negative mutants such as escherichia coli j5 and salmonella minnesota re595 is thought to neutralize the toxic effects of lipopolysaccharides (lpss). to verify that such antisera are capable of binding heterologous endotoxins, we examined igg and igm class antibodies induced in rabbits to a variety of lpss. immunization with rough mutants or lipid a induced high igg antibody responses to the homologous purified lps and relatively low but significant responses to heterolog ... | 1985 | 2413146 |
| the induction and characterization of rat liver stearyl-coa desaturase mrna. | poly(a+) rna isolated from livers of rats induced for stearyl-coa desaturase contains elevated levels of mrna for this enzyme which is translated in a rabbit reticulocyte system. the protein is immunologically and by peptide fingerprinting following staphylococcus aureus v8 protease digestion identical to the isolated enzyme and, therefore, not synthesized in a detectable larger precursor form. the desaturase mrna is selectively translated on free cytoplasmic polysomes from rat liver and represe ... | 1985 | 2414298 |
| immunogenicity and antigenicity of synthetic escherichia coli lipid a. | the immunogenicity and antigenicity of synthetic escherichia coli lipid a (compound 506) and its 1- and 4'-monophosphorylated derivatives (compounds 505 and 504, respectively) and nonphosphorylated derivative (compound 503) were compared with those of bis- and 4'-monophosphorylated natural free lipid a from e. coli. the synthetic compounds under study were either coated onto sheep erythrocytes (except for the water-insoluble preparation 503) or incorporated into liposomes and used for the immuni ... | 1986 | 2416687 |
| microbial and host factors that influence adherence of escherichia coli to kidney epithelium. | the adherence of escherichia coli 06k13h1 to punch biopsy specimens of rabbit renal pelvic tissue and isolated epithelial cells was examined quantitatively. organisms with pili adhered readily to kidney tissue, whereas organisms without pili (nonpiliated or grown in glucose-containing media) had significantly less adherence. adherence was inhibited by antibody to pili antigen but not by mannose (a determinant of adherence to buccal mucosal cells). studies were done to evaluate adherence under co ... | 1986 | 2422930 |
| cross-reactivity of monoclonal antibodies against lipopolysaccharides of gram-negative bacteria. | monoclonal antibodies were produced against escherichia coli o111, escherichia coli j5, and the rough (r) mutant of salmonella typhimurium m206, and tested by enzyme-linked immunosorbent assay against lipopolysaccharides of several gram-negative strains. the monoclonal antibodies were also identified with an immunoblotting assay. anti-escherichia coli o111 monoclonal antibodies reacted only with homologous o antigens. anti-j5 monoclonal antibodies cross-reacted with core lipopolysaccharide, espe ... | 1986 | 2424754 |
| [new data on the biological action of normal immunoglobulins]. | experiments on 2,520 cba mice (cba x x c57bl) f1 nice have shown that the injection of homologous serum immunoglobulins (obtained from intact and blood-stimulated animals), made 2 hours after gamma irradiation from a 60co source, prevents the development of intestinal dysbacteriosis and endogenous infection. the injection of mouse and human immunoglobulins to nonirradiated mice improved their resistance to experimental infection with escherichia coli live culture, increased the expression of rec ... | 1986 | 2425516 |
| a reexamination of the o1 lipopolysaccharide antigen group of escherichia coli. | a total of 64 escherichia coli strains of the o1 serogroup were tested for the migration pattern of their lipopolysaccharides (lps) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. o1:k1 and o1:k51 strains of the omp5 outer membrane protein pattern possessed lps with a doublet pattern (o1a1) or the lowermost band of the o1a1 doublet (o1a2). o1:k1 strains of the omp9 pattern possessed lps referred to as o1a, which corresponded to the uppermost band of the o1a1 doublet pattern. a few ... | 1986 | 2426197 |
| colonization, virulence, and mucosal interaction of an enteropathogenic escherichia coli (strain rdec-1) expressing shigella somatic antigen in the rabbit intestine. | the group factor 3,4 somatic antigen was transferred by recombination from a donor strain of shigella flexneri type 2a to the escherichia coli o15 rabbit pathogen strain rdec-1. a hybrid clone (7482-1-1) that expressed only the s. flexneri 3,4 somatic antigen and a second clone (7482-1-7) from the same mating mixture that expressed only the o15 somatic antigen were compared for virulence in rabbits. the 7482-1-1 strain produced diarrhea in 10% of rabbits versus 84% for the 7482-1-7 strain (p les ... | 1986 | 2430026 |
| t gamma protein is expressed on murine fetal thymocytes as a disulphide-linked heterodimer. | during the search for genes coding for the mouse alpha and beta subunits of the antigen-specific receptor of mouse t cells we encountered a third gene, subsequently designated gamma. this gene has many properties in common with the alpha and beta genes, somatic assembly from gene segments that resemble the gene segments for immunoglobulin variable (v), joining (j) and constant (c) regions; rearrangement and expression in t cells and not in b cells; low but distinct sequence homology to immunoglo ... | 1987 | 2434855 |
| synthetic gene construct expressing a repeated and highly immunogenic epitope of the plasmodium falciparum antigen pf155. | the plasmodium falciparum-derived antigen pf155 contains two blocks of tandemly repeated amino acid sequences. a pair of complementary oligonucleotides, encoding the c-terminally located repeat val-glu-his-asp-ala-glu-glu-asn, were synthesized. the oligonucleotides were polymerized by ligation, and the resulting multimers were cloned into an expression vector. one construct that contained four copies of the repeat was expressed in escherichia coli. the product, a fusion protein, was soluble and ... | 1987 | 2434955 |
| synthesis of fusion proteins with multiple copies of an antigenic determinant of foot-and-mouth disease virus. | a series of four expression plasmids coding for fusion proteins containing foot-and-mouth disease virus (fmdv) sequences was constructed. the fusion proteins contain a large part of beta-galactosidase from escherichia coli preceded (n-terminal) by 1, 2, 4 or 8 repeats of the antigenic determinant of fmdv consisting of amino acids 137-162 of the capsid polypeptide vp1. all four fusion proteins were efficiently produced in e. coli host bacteria. immunization of rabbits resulted in fmdv-specific, n ... | 1986 | 2436976 |
| [possible functional role of the dd-domain of rna-dependent polymerases]. | an attempt to study the functional role of one of the most conservative domains found in all rna-dependent rna and dna polymerases of plant and animal viruses (the so called "dd-domain") was made. a structure similar to the "dd-domain" was found in a minor t7 phage tail protein--gpii. antibodies against this phage protein have been raised and used to probe "dd-domain" in molecules of avian myeloblastose virus reverse transcriptase and e. coli rna-dependent rna polymerase. the antibodies are show ... | 1987 | 2437438 |
| pilus-mediated interactions of the escherichia coli strain rdec-1 with mucosal glycoproteins in the small intestine of rabbits. | escherichia coli strain rdec-1 (serotype 015:nm) is an effacing adherent enteropathogen that binds to the intestine of rabbits in a manner morphologically identical to the binding of human enteropathogenic e. coli strains to human intestine. the rabbit enteropathogen adheres to mucosal enterocytes in vivo and to microvillus membranes in vitro. binding of rdec-1 to ileal brush borders and to m cells overlying peyer's patches is mediated by pili (fimbriae) expressed on the cell surface of bacteria ... | 1987 | 2442061 |
| cloning of mycoplasma pneumoniae dna and expression of p1-epitopes in escherichia coli. | a genomic library of mycoplasma pneumoniae was constructed by cloning partial sau3a-digested genomic dna into the expression plasmids pex1 to pex3. the recombinant clones were screened for production of m. pneumoniae p1-antigen by an in situ colony enzyme-linked immunosorbent assay (elisa) blot method with a monospecific rabbit antiserum raised against the surface protein p1. the length of the translated p1-sequence and the size of the inserted dna were determined. by comparison it was shown tha ... | 1987 | 2444565 |
| antigenic determinants of the cholera/coli family of enterotoxins. | hybridoma-derived monoclonal antibodies were raised to enterotoxins of the cholera family and to chimeric b-subunit proteins in which individual amino acid residues of a heat-labile, cholera-related enterotoxin from an escherichia coli strain of porcine origin (p-lt) were substituted with corresponding residues from such an enterotoxin from an e. coli strain of human origin (h-lt). single amino acid substitutions were found to have profound effects on the physicochemical behavior of the proteins ... | 1987 | 2446368 |
| [immunochemical study of modified forms of l-asparaginase]. | certain catalytic and immunological properties of l-asparaginase modified by polyglucin were studied. it was shown that the modified forms of l-asparaginase maintained high catalytic activity (km 0.80.10(-5)-1.89.10(-5) m) and at the same time appeared to be more resistant to inactivation under the effect of antibodies. | 1987 | 2449861 |
| immunological characterization of thioltransferase from pig liver. | polyclonal antibodies against pig liver thioltransferase were raised in a new zealand rabbit. these antibodies completely neutralized the thioltransferase activity of the homogeneous enzyme and that in the crude cytosolic homogenate at an equivalent titer. the antibodies also cross-reacted equally with calf thymus glutaredoxin and calf liver thioltransferase, but not with escherichia coli thioredoxin, suggesting that thioltransferase and glutaredoxin from the same species are identical. immunobl ... | 1988 | 2454232 |
| [direct detection in diarrheal stools of escherichia coli adhesion factors cfa/i, cfa/ii and e8775 by the india ink immune reaction]. | an india ink immune reaction was used for the direct detection, in diarrhoeal stools, of escherichia coli possessing the cfa/i, cfa/ii and e8775 fimbriae. with this method, a presumptive diagnosis of enterotoxigenic e. coli (etec) can rapidly be made. staining required the bivalency of rabbit anti-fimbriae igg; the f(ab')2 fragment was necessary. the reaction was impossible with purified fab fragment. a comparative study of this technique and detection by culture of etec strains showed good corr ... | 1987 | 2456774 |
| [specificity of the protective action of a ribosomal shigella vaccine and the absence of activity in the ribosomes from r mutants]. | the ribosomal preparations of s. sonnei and some other bacterial species were obtained by the method of differential centrifugation, and the specificity of their protective action was studied in the keratoconjunctivitis test on guinea pigs. the ribosomal preparations were introduced parenterally in a single injection, and their protective action was determined two weeks later by the challenge of the animals with s. sonnei virulent strain and the subsequent calculation of the efficiency index (ei ... | 1988 | 2464261 |
| purification and characterization of a phage-encoded cytotoxin from an escherichia coli o111 strain associated with hemolytic-uremic syndrome. | cytotoxin production by escherichia coli o111:h-strain hus-2 (hamburg) is associated with a temperate toxin-converting bacteriophage (tcp-111). e. coli laboratory strain c600 transduced and subsequently lysed by the phage produced and liberated large amounts of cytotoxin (ct111) which was purified by sequential chromatography. when compared with published procedures for toxin release from viable cells, lysis of the c600 culture by the phage was most effective. by sds-page ct111 as shiga toxin fr ... | 1988 | 2464885 |
| epitope specificities of murine monoclonal and rabbit polyclonal antibodies against enterobacterial lipopolysaccharides of the re chemotype. | murine monoclonal and rabbit polyclonal antibodies raised against the lipopolysaccharides (lps) of re mutants of salmonella minnesota, proteus mirabilis, and escherichia coli were serologically characterized. using natural re lps and natural and synthetic partial structures thereof, representing the 3-deoxy-d-manno-2-octulosonic acid (kdo) or lipid a region or both, the epitope specificities of four monoclonal antibodies were defined. clones 20 (immunoglobulin m [igm]) and 25 (igg3) recognize a ... | 1989 | 2474499 |
| expression of shiga toxin epitopes in e. coli immunological characterization. | synthetic oligodeoxynucleotides encoding for two peptides corresponding to residues 9-21 and 19-31 in the amino acid sequence of the b subunit of shiga toxin were prepared and inserted into ptoz plasmid, in phase with the lacz gene. the resultant vectors were used for transfection of e. coli. the bacteria containing the recombinant dna expressed the respective peptides in the form of fusion proteins with beta-galactosidase. the n-terminal region of the shiga toxin b subunit, containing the two p ... | 1989 | 2475438 |
| electrical properties and molecular architecture of the channel formed by escherichia coli hemolysin in planar lipid membranes. | a 107 kda hemolysin from escherichia coli is able to open pores in lipid membranes. by studying its interaction with planar phospholipid bilayers we have derived some structural information on the organization of the pore. we measured the current-voltage characteristic and the ion selectivity of the channel both in neutral membranes, made of egg phosphatidylcholine (pc) and in negatively charged membranes, made of a 1:1 mixture of pc with phosphatidylserine (ps). experiments were performed varyi ... | 1989 | 2477066 |
| molecular cloning of the streptococcus mutans gene specifying antigen a. | a gene encoding a streptococcus mutans surface protein antigen has been isolated from a strain gs-5 gene bank constructed via the streptococcus-escherichia coli shuttle vector psa3. this e. coli recombinant clone, designated 4b2, expressed s. mutans proteins, as shown by western immunoblot analysis with a specific rabbit antibody to s. mutans surface antigens. three bands were observed, including a 52-kilodalton (kda) protein (pi 5.7), a 29-kda protein (pi 4.2), and a 20-kda protein usually pres ... | 1989 | 2478472 |
| a high-molecular-weight fraction of smooth lipopolysaccharide in klebsiella serotype o1:k20 contains a unique o-antigen epitope and determines resistance to nonspecific serum killing. | the lipopolysaccharide (lps) o-antigen side chains of klebsiella serotype o1 have been studied by using mutants selected by resistance to a klebsiella bacteriophage designated o1-a. two classes of lps mutants were identified. the major group (90%) synthesized rough lps. the remaining 10% of the mutants produced a novel lps profile that lacked the highest-molecular-weight o-substituted molecules (hmw-lps) but still produced lower-molecular-weight o-substituted species (lmw-lps). by using antisera ... | 1989 | 2478478 |
| cloning, expression and nucleotide sequence of the gene fragment encoding an antigenic portion of the nucleoside triphosphate hydrolase of toxoplasma gondii. | toxoplasma gondii expresses high levels of an active nucleoside triphosphate hydrolase (ntpase; ec 3.6.1.3) with several unique properties. it has been detected as a circulating antigen in mice, making it an ideal candidate for diagnostic tests for toxoplasmosis. a cdna library constructed from t. gondii poly(a)+rna was made in lambda gt11. one hundred thousand members of this library were immunoscreened with a rabbit polyclonal antibody to the purified ntpase. six positive clones were subcloned ... | 1989 | 2482826 |
| primary structure and functional aspects of the gene coding for the second-largest subunit of rna polymerase iii of drosophila. | we have cloned and sequenced the gene coding for the second-largest subunit of rna polymerase iii of drosophila melanogaster (dmrp135). the gene, interrupted by two introns of 62 and 59 bp, respectively, codes for an mrna of 3.6 kb. as for other housekeeping genes transcription initiates at several sites (between positions -98 and -76) none of which is preceded by a clear tata sequence. the deduced polypeptide consists of 1129 amino acids with an aggregate molecular weight of 128 kda. the protei ... | 1989 | 2482932 |
| passive transfer of meningococcal group b polysaccharide antibodies to the offspring of pregnant rabbits and their protective role against infection with escherichia coli k1. | pregnant rabbits vaccinated with meningococcal group b polysaccharide complexed to outer membrane proteins (serotype 6) responded to produce igg, igm and iga anti-b polysaccharide antibodies, which were passively transferred to the offspring (igg preferentially) and could be detected in their sera immediately after birth. these antibody levels were sustained in the mothers but diminished in the offspring to background levels at day 22 after birth. in a subsequent experiment, rabbits immunized wi ... | 1989 | 2497598 |
| the effects of a new tissue plasminogen activator analogue, fb-fb-cf, on cerebral reperfusion in a rabbit embolic stroke model. | early fibrinolytic therapy with full molecular tissue plasminogen activator (t-pa) has been observed to be both angiographically and clinically effective when employed in animal stroke models. preliminary clinical trials with t-pa are in progress. it is possible to refine t-pa by developing fragments or analogues of the drug. using recombinant dna technology in the escherichia coli system, a t-pa analogue consisting of the catalytic fragment of t-pa and a dimer of the b fragment of staphylococca ... | 1989 | 2499244 |
| reduction of protein mixed disulfides (dethiolation) by escherichia coli thioredoxin: a study with glycogen phosphorylase b and creatine kinase. | the role of thioredoxin in the reduction of protein mixed disulfides (dethiolation) was studied by electrofocusing methodology with glycogen phosphorylase b and creatine kinase as substrates for the reaction. glycogen phosphorylase b was effectively dethiolated by escherichia coli thioredoxin with dithiothreitol as the reductant, while creatine kinase could not be dethiolated by this mechanism. the rate of dethiolation of phosphorylase b was dependent on the concentration of thioredoxin up to a ... | 1989 | 2500063 |
| thromboxane-mediated hypertension and vascular leakage evoked by low doses of escherichia coli hemolysin in rabbit lungs. | escherichia coli hemolysin has been implicated as a pathogenicity factor in extraintestinal e. coli infections including sepsis. in the present study the effects of intravascular administration of hemolysin were investigated in isolated blood-free perfused rabbit lungs. low concentrations of the toxin in the perfusate (0.05-5 hemolytic units/ml, corresponding to approximately 5-500 ng/ml), caused a dose- and time-dependent release of potassium, thromboxane a2, and prostaglandin i2, but not of la ... | 1989 | 2500455 |
| selective segmental hepatic necrosis produced by the shwartzman mechanism in rabbits. | in this study, a new experimental model for hepatic necrosis is presented using rabbits, and e. coli endotoxin as the shwartzman reagent. a segment of the liver was chosen as a target site for the univisceral shwartzman reaction. endotoxin-lipiodol emulsion was used as a preparative injection via the portal venous branch into the target segment by direct puncture. provocation was made by an intravenous injection of endotoxin 24 h later. a marked and sharply demarcated necrotic area was produced ... | 1989 | 2510399 |
| a preliminary study of the inhibiting mechanism of anisodamine on rabbit platelets activated by e. coli endotoxin. | in this study, we observed the endotoxin induced platelet aggregation, 5-ht and beta-g releases, cellular camp decrease, and also the changes of morphology and membrane fluidity of platelet. these changes play an important role in septic shock, especially in disseminated intravascular coagulation (dic) and respiratory distress syndrome (rds). after anisodamine (654) intervention all the parameters mentioned above were improved to a certain extent. this preliminary study of the inhibiting mechani ... | 1989 | 2517730 |
| studies of topoisomerase-specific antitumor drugs in human lymphocytes using rabbit antisera against recombinant human topoisomerase ii polypeptide. | a complementary dna fragment of the human dna topoisomerase ii gene was cloned into a t7 expression vector and overproduced in escherichia coli. rabbit polyclonal antibodies were raised against the recombinant topoisomerase ii polypeptide which corresponds to the c-terminal one-third of human topoisomerase ii polypeptide. using the antiserum, dna topoisomerase ii levels were measured by immunoblotting human lymphocytes following phytohemagglutinin (pha) stimulation. our results showed that the i ... | 1989 | 2536295 |
| site-specific antibodies against the prla (secy) protein of escherichia coli inhibit protein export by interfering with plasma membrane binding of preproteins. | genetic evidence indicates that the prla (secy) protein of escherichia coli functions as a membrane integrated signal sequence receptor in protein "export"--i.e., in protein translocation across (or integration into) the plasma membrane. we have raised antibodies in rabbits against two synthetic peptides representing the hydrophilic n- or c-terminal region of prla. using these antibodies as probes in cell fractionation experiments, we confirm that prla is an integral membrane protein of the plas ... | 1989 | 2538820 |
| ubiquitin fusion augments the yield of cloned gene products in escherichia coli. | despite the availability of efficient transcription and translation signals, some heterologous gene products are not adequately expressed when introduced into prokaryotes and eukaryotes. an expression system has been established in escherichia coli to increase the yield of cloned gene products, where the c terminus of ubiquitin was fused to the n terminus of unstable or poorly expressed proteins. fusion of ubiquitin to yeast metallothionein or to the alpha subunit of the adenylate cyclase-stimul ... | 1989 | 2539593 |