Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| cardiac ankyrin repeat protein is a marker of skeletal muscle pathological remodelling. | in an attempt to identify potential therapeutic targets for the correction of muscle wasting, the gene expression of several pivotal proteins involved in protein metabolism was investigated in experimental atrophy induced by transient or definitive denervation, as well as in four animal models of muscular dystrophies (deficient for calpain 3, dysferlin, alpha-sarcoglycan and dystrophin, respectively). the results showed that: (a) the components of the ubiquitin-proteasome pathway are upregulated ... | 2009 | 19143834 |
| high-density spore production of a b. cereus aquaculture biological agent by nutrient supplementation. | previous studies have demonstrated the efficacy of our bacillus cereus isolate (nrrl 100132) in reducing concentrations of nitrogenous wastes and inhibiting growth of fish pathogens. in vivo efficacy and tolerance to a range of physiological conditions in systems used to rear cyprinus carpio make this isolate an excellent candidate for aquaculture applications. production cost is an important consideration in development of commercially relevant biological products, and this study examines the o ... | 2009 | 19148635 |
| effects of fatiguing jumping exercise on mrna expression of titin-complex proteins and calpains. | eccentric exercise induced by electrostimulation increases mrna expression of titin-complex proteins in rodent skeletal muscle. in this study, mrna expression of titin, muscle lim protein (mlp), cardiac ankyrin repeat protein (carp), ankyrin repeat domain protein 2 (ankrd2), diabetes-related ankyrin repeat protein (darp), and calcium-activated proteinases, calpains, were investigated in human skeletal muscle after fatiguing jumping exercise. fatiguing jumping exercise did not change mrna express ... | 2009 | 19150862 |
| the major portal of entry of koi herpesvirus in cyprinus carpio is the skin. | koi herpesvirus (khv), recently designated cyprinid herpesvirus 3, is the causative agent of a lethal disease in koi and common carp. in the present study, we investigated the portal of entry of khv in carp by using bioluminescence imaging. taking advantage of the recent cloning of the khv genome as a bacterial artificial chromosome (bac), we produced a recombinant plasmid encoding a firefly luciferase (luc) expression cassette inserted in the intergenic region between open reading frame (orf) 1 ... | 2009 | 19153228 |
| effects of acute exposure to metribuzin on some hematological, biochemical and histopathological parameters of common carp (cyprinus carpio l.). | the aim of the study was to evaluate acute toxic effects of the preparation sencor 70 wg (metribuzin 70% w/v) on hematological, biochemical indices and histology of the common carp (cyprinus carpio l.). in carp exposed for 96 h to sencor 70 wg in the concentration of 250.2 mg/l, showed significantly lower (p<0.01) values of plasma total proteins, albumins, total globulins, triacylglycerols, lactate dehydrogenase, lactate, inorganic phosphate, hematocrit, hemoglobin concentration, mean erythrocyt ... | 2009 | 19159050 |
| long-term uvb irradiation affects the immune functions of carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss). | the effects of long-term, low-dose ultraviolet b (uvb) radiation on immune functions of two fish species representing different taxonomic groups, carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss), were assessed in this study. the fish were exposed to 7, 20 or 60 mj cm(-2) uvb three times per week, for 6 weeks. in carp, uvb exposure affected the respiratory burst activity of blood and head kidney phagocytes, differential blood leukocyte counts and blood chemistry. phytohemagglutinin ... | 2009 | 19161399 |
| role of waterborne copper on survival, growth and feed intake of indian major carp, cirrhinus mrigala hamilton. | the effect of copper on survival, growth and feed intake of indian major carp, cirrhinus mrigala (hamilton) fry (0.92 +/- 0.28 g) was studied for 60 days. survival rates of the fish exposed to control (0.02), 0.10, 0.15 and 0.23 mg l(-1) copper were 100%, 83 +/- 3%, 58 +/- 6%, and 50 +/- 4%, respectively. average daily growth was significantly (p < 0.05) lower at 0.15 and 0.23 mg l(-1) of copper. there was almost no growth at 0.23 mg l(-1) of copper. feed intake rates reduced significantly (p < ... | 2009 | 19165406 |
| transfer, distribution and bioaccumulation of microcystins in the aquatic food web in lake taihu, china, with potential risks to human health. | in this paper, accumulation and distribution of microcystins (mcs) was examined monthly in six species of fish with different trophic levels in meiliang bay, lake taihu, china, from june to november 2005. microcystins were analyzed by liquid chromatography electrospray ionization mass spectrometry (lc-esi-ms). average recoveries of spiked fish samples were 67.7% for mc-rr, 85.3% for mc-yr, and 88.6% for mc-lr. the mcs (mc-rr+mc-yr+mc-lr) concentration in liver and gut content was highest in phyt ... | 2009 | 19185334 |
| cyanophycin-degrading bacteria in digestive tracts of mammals, birds and fish and consequences for possible applications of cyanophycin and its dipeptides in nutrition and therapy. | to determine the susceptibility of cyanophycin granule polypeptide (cgp) to degradation by several mammalian, avian and fish gut flora. | 2009 | 19191948 |
| characteristics of sarcoplasmic proteins and their interaction with surimi and kamaboko gel. | this study examined the effect of adding common carp sarcoplasmic proteins (sp- p) on the gel characteristics of threadfin bream surimi and kamaboko while maintaining constant moisture and myofibrillar levels. based on the temperature sweep test, which is involved in heating of surimi gel from 10 to 80 degrees c to monitor the viscoelastic properties, at temperature range of 40 to 50 degrees c, the decrease level (depth of valley) in storage modulus (g') thermograph was in proportion to the conc ... | 2010 | 19200110 |
| a global repair operator for capacitated arc routing problem. | capacitated arc routing problem (carp) has attracted much attention during the last few years due to its wide applications in real life. since carp is np-hard and exact methods are only applicable for small instances, heuristics and metaheuristic methods are widely adopted when solving carp. this paper demonstrates one major disadvantage encountered by traditional search algorithms and proposes a novel operator named global repair operator (gro) to address it. we further embed gro in a recently ... | 2009 | 19211356 |
| isolation, amino-acid sequence, synthesis and biological properties of urotensin i from hippoglossoides elassodon. | abstract a 41-residue urotensin i neuropeptide (h-ui) was isolated from urophyses of the marine teleost hippoglossoides elassodon (the flathead sole). the peptide was recognized by its partial cross-reactivity in a radioimmunoassay developed for catostomus (sucker) ul (s-ui), and was purified by reversed-phase high-performance liquid chromatography. the amino-acid sequence was shown to be h-ser-glu-glu-pro-pro-met-ser-lle-asp-leu-thr-phe-his-met-leu-arg-asn-met-lle-his-arg-ala-lys-met-glu-gly-gl ... | 1990 | 19215432 |
| diverse cell-specific expression of myoglobin isoforms in brain, kidney, gill and liver of the hypoxia-tolerant carp and zebrafish. | myoglobin (mb) is famous as a muscle-specific protein--yet the common carp expresses the gene (cmb1) encoding this protein in a range of non-muscle tissues and also expresses a novel isoform (cmb2) in the brain. using a homologous antibody and riboprobes, we have established the relative amounts and cellular sites of non-muscle mb expression in different tissues. the amounts of carp myoglobin (cmb) in supernatants of different tissues were just 0.4-0.7% relative to that of heart supernatants and ... | 2009 | 19218513 |
| [modulation and function of calcium signaling in retinal horizontal cells.]. | calcium is one of the most versatile intracellular second messengers, which plays crucial roles in many intracellular signaling pathways. researches on intracellular calcium distribution, regulation and function are important for our understanding of cellular physiology. in this mini-review, the regulation of intracellular calcium signal in retinal horizontal cells and the relevant physiological functions were introduced based on the experiments carried out in our laboratory. intracellular calci ... | 2009 | 19224047 |
| comparative study of erythrocytes of polyploid hybrids from various fish subfamily crossings. | we have undertaken comparative studies of the number and phenotypes of erythrocytes in the peripheral blood of red crucian carp (rcc), blunt snout bream (bsb), and their hybrids, including triploids, tetraploids, and pentaploids. the results indicate that the mean nuclear volume of erythrocytes in peripheral blood increases regularly with increasing ploidy. furthermore, many more mature erythrocytes have a dumbbell nucleus in the peripheral blood of polyploid hybrids compared with their diploid ... | 2009 | 19225811 |
| distribution of growth hormone-releasing hormone-like peptide: immunoreactivity in the central nervous system of the adult zebrafish (danio rerio). | the distribution of growth hormone-releasing hormone-like peptides (ghrh-lp) in the central nervous system of the zebrafish was investigated by using immunohistochemical techniques with polyclonal antibodies. elisas showed that the antiserum raised against salmon (s)ghrh-lp recognized both zebrafish ghrh-lp1 and -2, whereas the antiserum raised against carp (c)ghrh-lp was more sensitive but detected only zebrafish ghrh-lp1. neither antiserum detected the true ghrh. large cells in the nucleus lat ... | 2009 | 19235874 |
| antibody response of two populations of common carp, cyprinus carpio l., exposed to koi herpesvirus. | common carp, cyprinus carpio l., exposed to koi herpesvirus (khv) may become persistently infected and populations containing such virus-infected individuals may transmit the virus to other fish when co-habited. detection of virus-infected fish in a population is thus critical to surveillance and control programmes for khv. a study was therefore designed to detect anti-khv serum antibodies, with an enzyme-linked immunosorbent assay, in common carp following experimental exposures to khv under va ... | 2009 | 19236553 |
| molecular cloning and tissue distribution of lipoprotein lipase full-length cdna from pengze crucian carp (carassius auratus var. pengze). | a full-length cdna coding lipoprotein lipase (lpl) was cloned from liver of adult pengze crucian carp (carassius auratus var. pengze) by rt-pcr and rapid amplification of cdna ends (race) approaches. the cdna obtained was 1877 bp long with a 1524 bp open reading frame (orf) encoding 507 amino acids, including a putative signal peptide of 23 amino acids long. the deduced amino acid sequence has a high similarity and shows similar structural features to lpl of other species. the lpl protein has a ... | 2009 | 19236937 |
| evaluation of the acute toxicity of profenofos and its effects on the behavioral pattern of fingerling common carp (cyprinus carpio l., 1758). | profenofos, an organophosphate insecticide is acetylcholinesterase inhibitor that has the potential to contaminate the ground water. the 96 h lc(50) value of profenofos was determined in 3-month-old fingerling common carp (cyprinus carpio) with a body weight 1.04 +/- 0.25 g and a body length 4.25 +/- 0.75 cm at 26 +/- 1 degrees c temperature. trimmed spearman-karber (tsk) software was used for the statistical analysis, which calculated the lc(50) value as 62.4 microg/l for three replicates of th ... | 2009 | 19242633 |
| is lipid peroxidation induced by the aqueous suspension of fullerene c60 nanoparticles in the brains of cyprinus carpio? | this study aimed to clarify whether fullerene c60 nanoparticles induced lipid peroxidation in cyprinus carpio brains. a stable well-characterized aqueous suspension of c60 nanoparticle (diameter: 50th and 95th percentiles, 36 and 95 nm respectively) with 0.1% tween80 solution was prepared by bead milling. lipid hydroperoxides (lpo) were measured in vitro in homogenized fish brain tissues containing 33 microg/mg-protein dispersed c60 nanoparticles under light and dark conditions to verify the lip ... | 2009 | 19245041 |
| polychlorinated dibenzo-p-dioxins, dibenzofurans and biphenyls in crucian carp (carassius auratus) from rivers in korea during 2000-2004. | concentrations of polychlorinated dibenzo-p-dioxins (pcdds) and polychlorinated dibenzofurans (pcdfs) were analyzed in freshwater fish (crucian carp) taken from 16 locations in nine rivers and a wetland in korea, during 2000-2004. dioxin-like polychlorinated biphenyls (dl-pcbs) were also analyzed in the same samples but over shorter period (2002-2004). concentrations on a wet weight basis for 3 years (2002-2004) ranged from below the detection limit (dl) to 0.36 pg teq g(-1) wet wt. for pcdds, b ... | 2009 | 19246071 |
| a hepatic protein modulates glucokinase activity in fish and avian liver: a comparative study. | glucokinase (gck) is a key enzyme involved in hepatic glucose metabolism as well as in glucose homeostasis regulation. in mammals, gck is regulated in vivo by a regulatory protein (gckr) through a nucleus-to-cytoplasm translocation enhanced by fructose 1-phosphate and counteracted by fructose 6-phosphate. there were no previous evidences in literature regarding the presence of gckr in livers of other vertebrates like fish and bird. accordingly, in the present study we assessed gckr presence in c ... | 2009 | 19247671 |
| bioenergetics of fish spermatozoa during semen storage. | this mini-review focuses on changes in atp and creatine phosphate concentrations in fish sperm under storage conditions. the storage of catfish sperm at 4 degrees c leads to atp depletion and decreased sperm motility. the rate of intracellular atp depletion can be diminished through the addition of energetic substrates to the sperm storage medium, with lactate + pyruvate being the most efficient substrates for maintaining atp concentrations in catfish sperm. the decrease in atp concentration is ... | 2009 | 19247796 |
| simultaneous determination of microcystin contaminations in various vertebrates (fish, turtle, duck and water bird) from a large eutrophic chinese lake, lake taihu, with toxic microcystis blooms. | this is the first to conduct simultaneous determination of microcystin (mc) contaminations in multi-groups of vertebrates (fish, turtle, duck and water bird) from lake taihu with microcystis blooms. mcs (-rr, -yr, -lr) in microcystis scum was 328 microg g(-1) dw. mcs reached 235 microg g(-1) dw in intestinal contents of phytoplanktivorous silver carp, but never exceeded 0.1 microg g(-1) dw in intestinal contents of other animals. the highest mc content in liver of fish was in carassius auratus ( ... | 2009 | 19249079 |
| time-dependent oxidative stress responses of crucian carp (carassius auratus) to intraperitoneal injection of extracted microcystins. | this study was conducted to investigate time-dependent changes in oxidative enzymes in liver of crucian carp after intraperitoneally injection with extracted microcystins 600 and 150 microg kg(-1) body weight. the results showed that activities of antioxidant enzymes, including superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase generally exhibited a rapid increase in early phase (1-3 h post injection), but gradually decreased afterwards (12-48 h) compared with the c ... | 2009 | 19252760 |
| surface ultrastructure of gill arches and gill rakers in relation to feeding of an indian major carp, cirrhinus mrigala. | the surface ultrastructure of the gill arches and the gill rakers of an herbivorous fish, cirrhinus mrigala was investigated by scanning electron microscopy. these structures show significant adaptive modifications associated with the food and feeding ecology of the fish. closely lying short gill rakers and narrow inter-raker channels on the gill arches are associated to filter and retain food particles. prominent epithelial protuberances on the gill rakers and the gill arches enable the taste b ... | 2009 | 19261316 |
| association between immune-associated disorders and adverse outcomes of ileal pouch-anal anastomosis. | autoimmune disorders (ads) frequently coexist with inflammatory bowel disease. the aim of the study was to determine whether coexisting ad in patients with ileal pouches increases the risk for chronic antibiotic-refractory pouchitis (carp) and other inflammatory conditions of the pouch. | 2009 | 19262522 |
| new insights into the evolution of ifns: zebrafish group ii ifns induce a rapid and transient expression of ifn-dependent genes and display powerful antiviral activities. | the ifns and their receptors have existed in early chordates for approximately 500 million years and represent the early elements in innate and adaptive immunity. both types i and ii ifns have been discovered in fish, and type i has recently been classified into two groups based on their primary protein sequences. however, the biological activities of fish ifns and their roles in infection are largely unknown. using the zebrafish and manageable bacterial (streptococcus iniae) and viral (spring v ... | 2009 | 19265122 |
| recombinant expression and affinity purification of snake venom gland parvalbumin in escherichia coli. | parvalbumins (pv) are small, acidic, water soluble and calcium-binding proteins generally present in muscular and nervous tissues. in the present study, we identified and characterized a cdna clone encoding pv, named aplpv, from a snake (agkistrodon piscivorus leucostoma) venom gland cdna library. aplpv belongs to ef-hand proteins with six alpha-helices constituting three ef-hand domains. the deduced amino acid sequence of aplpv is 91% and 68% identical to the previously characterized pvs of boa ... | 2009 | 19275943 |
| development and use of real-time reverse transcription-polymerase chain reaction assay to quantify cytochrome p4501a1 expression in american mink. | the distribution of natural populations of american mink is restricted to locales that are in proximity to aquatic ecosystems. because of the lipophilicity and persistence of polychlorinated biphenyls (pcbs) and reliance of mink on aquatic-based diets, mink at contaminated locales often bioacccumulate high levels of pcbs. in addition, in controlled laboratory studies, mink are highly sensitive at reproductive and developmental end points to the toxic effects of environmental pcb mixtures. it is ... | 2009 | 19277445 |
| sublethal cyfluthrin toxicity to carp (cyprinus carpio l.) fingerlings: biochemical, hematological, histopathological alterations. | this study measures a whole series of biological endpoints, and histopathological and behavioral changes of carp (cyprinus carpio l.) fingerlings in order to investigate the adverse effects of a sublethal concentration of 10 microg/l cyfluthrin for 48h and one week using the semi-static test system. the most significantly altered findings of this study was that total antioxidant status levels decreased in 48h, chloride levels decreased in one week, and sodium and phosphorous levels increased in ... | 2009 | 19286258 |
| two divergent leptin paralogues in zebrafish (danio rerio) that originate early in teleostean evolution. | we describe duplicate leptin genes in zebrafish (danio rerio) that share merely 24% amino acid identity with each other and only 18% with human leptin. we were also able to retrieve a second leptin gene in medaka (oryzias latipes). the presence of duplicate leptin genes in these two distantly related teleosts suggests that duplicate leptin genes are a common feature of teleostean fishes. despite low primary sequence conservation, we are confident in assigning orthology between mammalian and zebr ... | 2009 | 19293295 |
| impact of copper on the oxidative metabolism of the fry of common carp, cyprinus carpio (linn.) at different ph. | in order to evaluate the impact of copper on the energetics of a fish, the levels of glucose, glycogen, pyruvate and lactate, the rate of tissue oxygen consumption and the activities of glycogen phosphorylase, isocitrate dehydrogenase (icdh), succinate dehydrogenase (sdh) and lactate dehydrogenase (ldh) were estimated in the whole body of the fry of cyprinus carpio immediately after 1, 7, 15 and 30 days on exposure to a sublethal concentration of copper 0.08 mgl(-1) at ph 7.5 (normal), 6.0 (weak ... | 2008 | 19295071 |
| involvement of gadd153 and cardiac ankyrin repeat protein in cardiac ischemia-reperfusion injury. | oxidative stress is critical for causing cardiac injuries during ischemia-reperfusion (ir), yet the molecular mechanism for this remains unclear. in the present study, we observe that hypoxia and reoxygenation, a component of ischemia, effectively induces apoptosis in the cardiac myocytes from neonatal rats and it concomitantly leads to induction of gadd153, an apoptosis-related gene. furthermore, ir injury of rat heart showed a gadd153 overexpression in the ischemic area where the tunel reactio ... | 2009 | 19299913 |
| differential binding of mammalian and salmon gnrhs with rat and carp pituitary receptors. | receptor binding of gnrh is connected with the stimulation of pituitary gonadotropic cells leading to both the release and biosynthesis of gonadotropins. the binding is connected with the conformational changes in the receptor which induce the specific intracellular signalisation. the study of fish gnrhs and their receptors may give us new knowledge of the complex interplay of different mechanisms involved in neuroendocrine regulation of reproduction. | 2009 | 19300396 |
| etiology and pathology of epidermal papillomas in allogynogenetic crucian carp carassius auratus gibelio (female) x cyprinus carpio var. singuonensis (male). | allogynogenetic crucian carp carassius auratus gibelio (female) x cyprinus carpio var. singuonensis (male) is one of the main freshwater aquaculture species in china. in recent years, epidermal papillomas have been observed on the scales, fins and opercula of adult fish in many fish farms in the chongming county of shanghai, china. the disease appears in the late autumn of the first year and becomes more severe in winter. it gradually regresses in the late spring or summer of the second year, as ... | 2009 | 19301639 |
| scared fish get lazy, and lazy fish get fat. | 1. many biological textbooks present predator-induced morphological changes in prey species as an example of an adaptive response, because the morphological change is associated with lower predation risk. here we show that the adaptive morphological response observed in many systems may actually be an indirect effect of decreased activity - which reduces the predation risk - rather than a direct adaptive response. 2. one of the classical examples comes from crucian carp, where the presence of pi ... | 2009 | 19302323 |
| no need to open the jar: a comparative study of magnetic resonance imaging results on fresh and alcohol preserved common carps (cyprinus carpio (l. 1758), cyprinidae, teleostei). | magnetic resonance imaging (mri) examinations have been conducted both on fresh and alcohol-preserved common carps (cyprinus carpio). the images have been compared to those of a sagittally-cut frozen animal of the same species. this work shows that the images obtained are globally similar, and that the mri technique can be applied to investigate the gross anatomy of alcohol-preserved specimens without destroying them. unfortunately, this kind of study does not provide precise enough anatomical d ... | 2009 | 19304272 |
| first description of a novel weissella species as an opportunistic pathogen for rainbow trout oncorhynchus mykiss (walbaum) in china. | six strains of gram-positive, catalase-negative, non-motile, irregular short rod-shaped weissella bacteria, with width and length of 0.5-0.6 and 1.2-2.7microm were isolated from diseased rainbow trout oncorhynchus mykiss (walbaum) in winter of 2007 at a commercial fishery in jingmen, hubei province, china. the diseased rainbow trout exhibited hemorrhage in eyes, anal region, intestine and abdomen wall, petechia of liver, some fish with hydrocele in stomach. six isolates had identical biochemical ... | 2009 | 19321272 |
| proteomic changes in the crucian carp brain during exposure to anoxia. | during exposure to anoxia, the crucian carp brain is able to maintain normal overall protein synthesis rates. however, it is not known if there are alterations in the synthesis or expression of specific proteins. this investigation addresses this issue by comparing the normoxic and anoxic brain proteome. nine proteins were found to be reduced by anoxia. reductions in the glycolytic pathway proteins creatine kinase, fructose biphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, trioseph ... | 2009 | 19322784 |
| spectacular morphological novelty in a miniature cyprinid fish, danionella dracula n. sp. | danionella dracula is a new species of sexually dimorphic, miniature and highly developmentally truncated cyprinid fish. compared with its close relative, the zebrafish danio rerio, it lacks 44 bones or parts thereof and represents one of the most developmentally truncated vertebrates. absence of the majority of bones appears to be due to developmental truncation via terminal deletion. in contrast to these larval-like features, d. dracula also shows several hyperossifications. uniquely, among ca ... | 2009 | 19324738 |
| characterization of a pattern recognition molecule vitellogenin from carp (cyprinus carpio). | pattern recognition proteins function in innate immune responses by binding to molecules on the surface of invading pathogens and initiating host defense reactions. to explore the role of vitellogenin (vg) in fish innate immunity, we purified vg from carp by gel filtration combined with diethylaminoethyl (deae) chromatography. the purified vg was confirmed by maldi-tof mass spectrometry. antibacterial activity analysis showed that vg inhibited bacterial activity to escherichia coli and staphyloc ... | 2009 | 19327543 |
| resistance of common carp (cyprinus carpio l.) to cyprinid herpesvirus-3 is influenced by major histocompatibility (mh) class ii b gene polymorphism. | the role of mh class ii b (cyca-dab1-like) genes in resistance of common carp (cyprinus carpio l.) to cyprinid herpesvirus-3 (cyhv-3), also known as koi herpesvirus (khv) was analysed. the material consisted of 934 fish from six carp crosses. fish were challenged with cyhv-3 at an age of 7 and 10 months. during challenge experiments the peak of mortality caused by cyhv-3 was observed at days 8-12 p.i. and the overall cumulative mortality reached 79.9%. among six cyca-dab1-like genotypes, reveale ... | 2009 | 19328856 |
| the effects of ghrelin on the in vitro spontaneous and sgnrh-a stimulated luteinizing hormone (lh) release from the pituitary cells of common carp (cyprinus carpio l.). | in fish, like in mammals, ghrelin affects gonadotropin release acting at the level of the hypothalamus as well as directly on the pituitary gland. in the present study, enzymatically dispersed pituitary cells obtained from sexually mature male and female carp (cyprinus carpio l.) were incubated in the presence of human ghrelin at the concentration of 10(-7) or 10(-6) m, salmon gnrh analogue (des-gly(10), d-arg(6), trp(7), leu(8), pro(9))-lhrh (sgnrh-a) at the concentration of 10(-8) m or the com ... | 2009 | 19328862 |
| increased liver protein and mrna expression of natural killer cell-enhancing factor b (nkef-b) in ayu (plecoglossus altivelis) after aeromonas hydrophila infection. | natural killer cell-enhancing factor (nkef) may mediate cellular responses to proinflammatory molecules. the liver proteins of aeromonas hydrophila-infected ayu (plecoglossus altivelis) and healthy control fish were analyzed by 2de. a protein, which increased significantly in diseased fish, was identified as nkef-b by maldi-tof-ms. a full-length cdna clone of this proteinwas subsequently isolated. it contains 1092 bp with an open reading frame of 591 bp, coding for 197 amino acids with mw 21.9 k ... | 2009 | 19340957 |
| x-ray microanalysis (edxma) of cadmium-exposed eggs of bothriocephalus acheilognathi (cestoda: bothriocephalidea) and the influence of this heavy metal on coracidial hatching and activity. | over recent years it has been established that pollutants can have a significant impact on host-parasite systems in the aquatic environment, so much so that it has been proposed that parasite fauna may be a useful parameter to monitor water quality. surprisingly, with perhaps the exception of trematodes and bioaccumulation in adult acanthocephalans, detailed observations on the interaction between helminths, particularly cestodes, and pollutants such as heavy metals, are lacking. in this study, ... | 2009 | 19341741 |
| intron retention generates ankrd1 splice variants that are co-regulated with the main transcript in normal and failing myocardium. | the cardiac ankyrin repeat domain 1 protein (ankrd1, also known as carp) has been extensively characterized with regard to its proposed functions as a cardio-enriched transcriptional co-factor and stress-inducible myofibrillar protein. the present results show the occurrence of alternative splicing by intron retention events in the pig and human ankrd1 gene. in pig heart, ankrd1 is expressed as four alternatively spliced transcripts, three of which have non-excised introns: ankrd1-contained intr ... | 2009 | 19341785 |
| purification and characterization of alpha(1)-proteinase inhibitor and antithrombin iii: major serpins of rainbow trout (oncorhynchuss mykiss) and carp (cyprinus carpio) blood plasma. | the main serine proteinase inhibitors of rainbow trout (oncorhynchuss mykiss) and common carp (cyprinus carpio) blood plasma were isolated and purified. the investigated inhibitors, alpha(1)-proteinase inhibitor (alpha(1)-pi) and antithrombin iii (at iii), act by forming stable complexes with target proteinases. the association rate constants k (on) for the interaction of fish plasma inhibitors with several serine proteinases have been determined: k (on) for both carp and rainbow trout alpha(1)- ... | 2009 | 19343519 |
| purification and partial characterization of vitellogenin from shorthead redhorse (moxostoma macrolepidotum) and copper redhorse (moxostoma hubbsi) and detection in plasma and mucus with a heterologous antibody. | vitellogenin (vtg), the egg yolk precursor protein, was purified from plasma of estradiol-3-benzoate (e2b)-treated male shorthead redhorse (moxostoma macrolepidotum) and immature copper redhorse (moxostoma hubbsi) by a two-step chromatographic procedure without precipitation. intact vtgs appeared as dimers with apparent molecular masses, determined by gel filtration, of approximately 425 kda (copper redhorse) and approximately 450 kda (shorthead redhorse). in native polyacrylamide gel electropho ... | 2009 | 19343520 |
| identification of a splice variant of the metal-responsive transcription factor mtf-1 in common carp. | transactivation of the expression of metallothionein genes involves the metal-responsive transcription factor (mtf-1). we report here the identification of mtf-1.1a, the first known splice variant of mtf-1.1 mrna, in common carp (cyprinus carpio). the lack of a 103 nt internal segment results in a frame shift, causing the early termination of translation. mtf-1.1a mrna encodes a protein consisting of the first 349 amino acids of mtf-1.1 plus an additional 64 amino acids, with no significant simi ... | 2009 | 19345278 |
| identification of two phospholipid hydroperoxide glutathione peroxidase (gpx4) genes in common carp. | the monomeric selenoprotein, phospholipid hydroperoxide glutathione peroxidase (gpx4) is an essential member of the antioxidant defense system. this paper describes the identification of two gpx4 genes (gpx4a and gpx4b) from somatic tissues of common carp (cyprinus carpio). the two sequences exhibited 78% and 79% identity at the dna and the predicted protein level, respectively. the gpx4a transcript was detected in all examined tissues of unstressed animals, with the highest level in the liver. ... | 2009 | 19345279 |
| in vitro hepatic metabolism of 2,2',4,4',5-pentabromodiphenyl ether (bde 99) in chinook salmon (onchorhynchus tshawytscha). | polybrominated diphenyl ethers (pbdes) are brominated flame retardants that persist in the environment and are present in geographically widespread fish species. pbde concentrations can be particularly high in resident chinook salmon (onchorhynchus tshawytscha) in the puget sound, washington. although pbde residues in salmon and other fish are often dominated by lower brominated congeners, these congeners are not produced commercially in the greatest quantity, suggesting bioaccumulation of the l ... | 2009 | 19346012 |
| vitellogenesis in the cestode atractolytocestus huronensis anthony, 1958 (caryophyllidea: lytocestidae). | vitellogenesis in the parthenogenetic caryophyllidean tapeworm atractolytocestus huronensis anthony, 1958, parasitizing the carp cyprinus carpio l., has been examined using light (lm) and transmission electron microscopy and cytochemical staining with periodic acid-thiosemicarbazide-silver proteinate for glycogen. it was found that formation of vitellocytes shares the same basic patterns in lower tapeworms with parthenogenetic and normal reproduction. each vitelline follicle of a. huronensis con ... | 2009 | 19352702 |
| function of metallothioneins in carp cyprinus carpio from two field sites in western ukraine. | the aim of the present study was to elucidate the seasonal and spatial regularity of the properties of metallothioneins (mt) from the liver and gills of carp cyprinus carpio l. in rural (r) and industrial (i) sites in western ukraine. the mt is represented by two chromatographic forms, the features of which exhibit seasonal rather than spatial dependence. the pronounced differences between the sites were due to the lower levels of zn in the liver and the higher levels of zn in mt of carp from si ... | 2009 | 19356800 |
| upregulated expression of cardiac ankyrin repeat protein in human failing hearts due to arrhythmogenic right ventricular cardiomyopathy. | expression of cardiac ankyrin repeat protein (carp) is augmented in heart failure due to dilated or ischaemic cardiomyopathy. it is unclear whether carp is upregulated in heart failure due to arrhythmogenic right ventricular cardiomyopathy (arvc). in the present study, we investigated the expression pattern of carp and the correlation between carp and the well-known heart failure marker pro-atrial natriuretic peptide (proanp) in arvc failing hearts. | 2009 | 19359327 |
| [investigations of ectoparasite fauna of some fish species (cyprinus carpio linnaeus, 1758; cobitis simplicispinna hanko, 1924) from lake akşehir (konya)]. | in this study, the presence of metazoan ectoparasites on 7 cyprinus carpio and 90 cobitis simplicispinna from lake aksehir, turkey were investigated between july 2004 and june 2005. three parasite species were identified on the host fishes: gyrodactylus elegans nordmann, 1832 (42.9%, 293.6+/-482.0 parasite/fish) and dactylogyrus extensus mueller and van cleave, 1932 (85.7%, 9.8+/-6.8), were found on gills of c. carpio, and gyrodactylus cobitis bychowsky, 1933 (68.9%, 15.6+/-18.5) on gills and fi ... | 2009 | 19367558 |
| compositional characteristics of materials recovered from whole gutted silver carp (hypophthalmichthys molitrix) using isoelectric solubilization/precipitation. | isoelectric solubilization/precipitation (isp) at acidic and basic ph was applied to whole carp, yielding proteins, lipids, and insolubles. the objective was to characterize composition of recovered materials. crude protein was concentrated to 89-90% in proteins recovered at acidic ph and to 94-95% at basic ph. basic ph yielded proteins with more (p < 0.05) essential amino acids (eaas). eaa content in recovered proteins met fao/who/uno requirements. isp did not affect fatty acid (fa) composition ... | 2009 | 19368395 |
| the immunogenicity of viral haemorragic septicaemia rhabdovirus (vhsv) dna vaccines can depend on plasmid regulatory sequences. | a plasmid dna encoding the viral hemorrhagic septicaemia virus (vhsv)-g glycoprotein under the control of 5' sequences (enhancer/promoter sequence plus both non-coding 1st exon and 1st intron sequences) from carp beta-actin gene (pae6-g(vhsv)) was compared to the vaccine plasmid usually described the gene expression is regulated by the human cytomegalovirus (cmv) immediate-early promoter (pmcv1.4-g(vhsv)). we observed that these two plasmids produced a markedly different profile in the level and ... | 2009 | 19368775 |
| [influence of stress hormones (adrenalin and glucocorticoids) on the infestation rate of the carp cyprinus carpio l. by dactylogyrus sp. (monogenoidea)]. | influence of adrenaline, hydrocortisone, and prednisolone on the infestation rate of the carp cyprinus carpio by dactylogyrus sp. is investigated, according to the analysis of the abudance index of parasites. introduction of these hormones into experimental fish resulted in the increase of the abudance index of dactylogyrids. dependence of changes in the investigated parameters on the nature and character of the hormones' influence on the host immune system and metabolic processes is revealed. | 2009 | 19370984 |
| co-culture of carp (cyprinus carpio) kidney haematopoietic cells with feeder cells resulting in long-term proliferation of t-cell lineages. | to characterise fish haematopoietic stem/progenitor cells, it is necessary to develop a culture system that supports proliferation and differentiation of these cells. in the present study, we established cell lines from various tissues of carp (cyprinus carpio) and ginbuna (carassius auratus langsdorfii). by using these cell lines, we developed a culture system in which carp haematopoietic cells proliferated and were successively passaged. cell lines from carp thymus (kot), carp fin (kof1) and g ... | 2009 | 19375173 |
| quantitative structure-activity relationship modeling of the toxicity of organothiophosphate pesticides to daphnia magna and cyprinus carpio. | within the reach regulatory framework in the eu, quantitative structure-activity relationships (qsar) models are expected to help reduce the number of animals used for experimental testing. the objective of this study was to develop qsar models to describe the acute toxicity of organothiophosphate pesticides to aquatic organisms. literature data sets for acute toxicity data of organothiophosphates to fish and one data set from experiments with 15 organothiophosphates on daphniamagna performed in ... | 2009 | 19376559 |
| screening for coping style increases the power of gene expression studies. | individuals of many vertebrate species show different stress coping styles and these have a striking influence on how gene expression shifts in response to a variety of challenges. | 2009 | 19390591 |
| three-dimensional study on the effect of organophosphate pesticide 'monocrotophos' on lens of fish and its recovery. | the present studies are based on the feedback provided by fish farmers practicing composite fish culture of carps. according to them, fish habitats are affected by pesticides draining from agricultural fields into their catchments, especially during the rainy season. the lenses of most of the cultured fish became opaque, resulting in poor overall growth. in the literature, there was mounting evidence of pesticide toxicity involving multiple tissues. however, the fish visual system has never been ... | 2009 | 19392874 |
| new insights into seafood allergy. | seafood plays an important role in human nutrition worldwide, sustained by international trade of a variety of new seafood products. increased production and consumption have resulted in more frequent reports of adverse reactions, highlighting the need for more specific diagnosis and treatment of seafood allergy. this review discusses recent literature in this field. | 2009 | 19398906 |
| a novel male-specific dna sequence in the common carp, cyprinus carpio. | in this study, we identified a sex-specific dna marker using randomly amplified polymorphic dna (rapd) fingerprinting in yellow river carps (cyprinus carpio from the yellow river). two hundred and twenty random primers were used in pooled dna samples and individual dna samples from male and female fish for rapd fingerprinting. when using the primer s2107, a novel sex-specific pcr product was identified in all male individuals. dna sequencing revealed that this 909 bp long dna fragment has a low ... | 2009 | 19401231 |
| common carp (cyprinus carpio) response to two pieces of music ("eine kleine nachtmusik" and "romanza") combined with light intensity, using recirculating water system. | the objective of this study was to further investigate the effects of music on fish physiology, bearing in mind available information regarding the involvement of endogenous and exogenous factors in fish farming. therefore, cyprinus carpio (50.5 +/- 0.36 g) were reared in a recirculating water system under 80 and 200 lux and subjected to no music at all (control, ambient noise only), 4 h of mozart's "eine kleine nachtmusik", or 4 h of anonymous "romanza-jeux interdits" for 106 days. both music t ... | 2010 | 19408132 |
| toxic effects of cobalt chloride on hematological factors of common carp (cyprinus carpio). | in this study, we investigate the toxic effects of cobalt chloride on some hematological factors of the carp cyprinus carpio, such as white blood cell count, red blood cell count, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration. at first, lc50 of cobalt in c. carpio was measured during 96 h after exposure. also, physicochemical parameters of water including ph, dissolved oxygen, viscosity, temperature, and conductivity w ... | 2009 | 19415186 |
| molecular connectivity indices for predicting bioactivities of substituted nitrobenzene and aniline compounds. | bioconcentration and toxicity of 22 substituted nitrobenzene and aniline compounds to aquatic organisms were assessed with quantitative structure-activity relationship (qsar). acute toxicities of aquatic organisms, including daphnia (daphnia pulex) and carp (cyprinus carpio), to 22 chemicals have been determined in our previous work. in this study, the bioconcentration factors (bcfs) for carp were further investigated. by the use of multiple-regression analyses, the molecular connectivity indice ... | 2009 | 19423164 |
| spatially explicit modeling of habitat dynamics and fish population persistence in an intermittent lowland stream. | in temperate and arid climate zones many streams and rivers flow intermittently, seasonally contracting to a sequence of isolated pools or waterholes over the dry period, before reconnecting in the wetter parts of the year. this seasonal drying process is central to our understanding of the population dynamics of aquatic organisms such as fish and invertebrates in these systems. however, there is a dearth of empirical data on the temporal dynamics of such populations. we describe a spatially exp ... | 2009 | 19425435 |
| in vitro generation of viral-antigen dependent cytotoxic t-cells from ginbuna crucian carp, carassius auratus langsdorfii. | little is known about antigen-specific t-cell responses to viruses in teleosts due to a lack of a suitable experimental system using inbred or clonal animals. in the present study we have successfully induced an in vitro generation of virus-specific cytotoxic t-cells (ctls) from isogeneic ginbuna crucian carp. responder cells (primarily lymphocytes) from crucian carp haematopoietic necrosis virus (chnv)-infected fish were capable of proliferating after stimulation in vitro with chnv-infected syn ... | 2009 | 19428040 |
| characterisation of a carp cell line for analysis of apoptosis. | teleost fish in general, and common carp in particular, are excellent genetic models for bridging the gap in knowledge between invertebrate models such as c. elegans and d. melanogaster, on one hand, and higher vertebrates on the other hand, although, until now, there have been few well characterised fish cell lines shown to be suitable for studies on apoptosis. the present study describes the suitability of a permanent, nonleukemic, nonvirally infected carp cell line for apoptotic studies. a tr ... | 2009 | 19428480 |
| differentiation between cyprinid herpesvirus type-3 lineages using duplex pcr. | to date, all the isolates of cyprinid herpesvirus type-3 (cyhv3) responsible for serious outbreaks in carps cyprinus carpio have been found to be very similar or identical on the basis of dna sequences of a few reference genes. however, two genetic lineages (u/i and j) are distinguished by full-length genome sequencing. two molecular markers presenting genetic variations were targeted for developing a duplex pcr assay able to distinguish cyhv3-u/i from cyhv3-j while avoiding dna sequencing. the ... | 2009 | 19428569 |
| new tools to study rna interference to fish viruses: fish cell lines permanently expressing sirnas targeting the viral polymerase of viral hemorrhagic septicemia virus. | previous studies have indicated that low transfection efficiency can be a major problem when gene inhibition by the use of small interfering rnas (sirnas) is attempted in fish cells. this may especially be true when targeting genes of viruses which are fast replicating and which can still infect cells that have not been transfected with the antiviral sirnas. to increase the amount of antiviral sirnas per cell a different strategy than transfection was taken here. thus, we describe carp epithelio ... | 2009 | 19428606 |
| in vitro biotransformation of surfactants in fish. part ii--alcohol ethoxylate (c16eo8) and alcohol ethoxylate sulfate (c14eo2s) to estimate bioconcentration potential. | recent regulatory pressures (e.g., reach, cepa) requiring bioaccumulation assessments and the need for reduced animal use have increased the necessity for the development of in vitro-based methods to estimate bioaccumulation. our study explored the potential use of subcellular and cellular hepatic systems to determine the biotransformation potential of two surfactants: octaethylene glycol monohexadecyl ether (c16eo8) and diethylene glycol monotetradecyl ether sulfate (c14eo2s). the subcellular s ... | 2009 | 19433333 |
| immunostimulatory effects of natural human interferon-alpha (huifn-alpha) on carps cyprinus carpio l. | human interferon-alpha (huifn-alpha) is an important immunomodulatory substance used in the treatment and prevention of numerous infectious and immune-related diseases in animals. however, the immunostimulatory effects of huifn-alpha in fish remain to be investigated. in the current study, the immune responses of the carp species cyprinus carpio l. to treatment with huifn-alpha were analyzed via measurement of superoxide anion production, phagocytic activity and the expression of cytokine genes ... | 2009 | 19439367 |
| the antioxidant glutathione in the fish cell lines epc and bcf-2: response to model pro-oxidants as measured by three different fluorescent dyes. | reduced glutathione (gsh) protects cells against injury by oxidative stress and maintains a range of vital functions. in vitro cell cultures have been used as experimental models to study the role of gsh in chemical toxicity in mammals; however, this approach has been rarely used with fish cells to date. the present study aimed to evaluate sensitivity and specificity of three fluorescent dyes for measuring pro-oxidant-induced changes of gsh contents in fish cell lines: monochlorobimane (mbcl), 5 ... | 2009 | 19444932 |
| progestogens cause immunosuppression of stimulated carp (cyprinus carpio l.) leukocytes in vitro. | the involvement of steroid hormones in direct and indirect regulation and modulation of immune responses is well recognized in mammals. here, we demonstrate that progestogens are capable of influencing the innate immunity in fish as well. therefore, we confirmed the known immunosuppressive effects of natural progesterone (p4), and compared them to influences of 17alpha,20beta-dihydroxy progesterone (dhp4) and the synthetic progestins, medroxyprogesterone acetate (mpa) and levonorgestrel (lev), o ... | 2009 | 19444990 |
| n-acetylcysteine provides dose-dependent protection against fenthion toxicity in the brain of cyprinus carpio l. | n-acetyl-l-cysteine, a low-molecular weight thiol compound, with two different doses was used to prevent fenthion, an organophosphorus insecticide and acaricide, related oxidative stress in the brain of a model organism, cyprinus carpio. fish were exposed to sub-lethal and nominal concentration of fenthion after intraperitoneal injection of 0.5 or 400 mg/kg nac. brain tissues were then dissected and homogenized to analyse gsh, gssg, tbars, and protein contents. enzymes that constitute the first ... | 2009 | 19444991 |
| differences in the antibody response and survival of genetically different varieties of common carp (cyprinus carpio l.) vaccinated with a commercial aeromonas salmonicida/a. hydrophila vaccine and challenged with a. hydrophila. | males of two strains of carp, wild duna (d), and inbred szarvas 22 (22), were selected for high and low stress response. two purebreds of d and 22, from randomly chosen parents and four crosses, 22 x 22-l (low stress response), 22 x 22-h (high stress response), 22 x d-l (low stress response) and 22 x d-h (high stress response) from selected stress response parents were produced and vaccinated with a commercial aeromonas salmonicida/aeromonas hydrophila vaccine and their circulating antibody resp ... | 2009 | 19449165 |
| function of the opioid system during inflammation in carp. | the opioid system is involved in modulation of both innate and acquired immune responses, thus altering resistance to a variety of infectious agents. we sequenced and characterized carp opioid receptors (mor, dor, and kor) and found their regulated expression in piscine leukocytes. moreover, both in vivo and in vitro opioids affect activity of leukocytes and expression of inflammatory molecules, especially chemokines and chemokine receptors. our data indicate an evolutionary conserved role for t ... | 2009 | 19456406 |
| transcript levels of five enzymes involved in cortisol synthesis and regulation during the stress response in common carp: relationship with cortisol. | in this study the expression of five genes involved in cortisol synthesis and regulation in the head kidneys of common carp (cyprinus carpio l.) has been investigated in response to 3h net confinement stress, followed by 22h recovery. cortisol, glucose, lactate and free fatty acid levels were measured in blood plasma. star, p450c17a2, 3betahsd, p450c21 and 11betahsd2 transcript sequences were identified based on cyprinidae homologs and quantified by real-time pcr. results showed that the plasma ... | 2009 | 19463824 |
| glucose-6-phosphate isomerase is an endogenous inhibitor to myofibril-bound serine proteinase of crucian carp (carassius auratus). | glucose-6-phosphate isomerase (gpi) was purified to homogeneity from the skeletal muscle of crucian carp ( carassius auratus ) by ammonium sulfate fractionation, column chromatographies of q-sepharose, sp-sepharose, and superdex 200 with a yield of 8.0%, and purification folds of 468. the molecular mass of gpi was 120 kda as estimated by gel filtration, while on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), two subunits (55 and 65 kda) were identified, suggesting that it ... | 2009 | 19472999 |
| determination of microcystin-lr and its metabolites in snail (bellamya aeruginosa), shrimp (macrobrachium nipponensis) and silver carp (hypophthalmichthys molitrix) from lake taihu, china. | this paper describes seasonal changes of microcystin-lr (mc-lr) and its glutathione (mc-lr-gsh) and cysteine conjugates (mc-lr-cys) in three aquatic animals--snail (bellamya aeruginosa), shrimp (macrobrachium nipponensis) and silver carp (hypophthalmichthys molitrix) collected from lake taihu, china. mc-lr, mc-lr-gsh, and mc-lr-cys were determined by liquid chromatography electrospray ionization mass spectrum (lc-esi-ms). the mean mc-lr concentrations in the hepatopancreas of snail and shrimp an ... | 2009 | 19473685 |
| spatiotemporal trends in fish mercury from a mine-dominated ecosystem: clear lake, california. | clear lake, california, usa, receives acid mine drainage and mercury (hg) from the sulphur bank mercury mine, a u.s. environmental protection agency (u.s. epa) superfund site that was active intermittently from 1873 to 1957 and partially remediated in 1992. mercury concentrations were analyzed primarily in four species of clear lake fishes: inland silversides (menidia beryllina, planktivore), common carp (cyprinus carpio, benthic scavenger/omnivore), channel catfish (ictalurus punctatus, benthic ... | 2008 | 19475924 |
| in vivo exposure of susceptible and non-susceptible fish species to myxobolus cerebralis actinospores reveals non-specific invasion behaviour. | due to controversial previous results, it has been unclear whether myxobolus cerebralis (myxosporea, myxozoa) is able to specifically choose a salmonid host by selective attachment and penetration. using a novel in vivo tracking assay that employs fluorescence staining of actinospore sporoplasms, we demonstrate a lack of host specificity of m. cerebralis actinospores during their initial invasion reactions. fish were experimentally exposed to stained actinospores that could be detected as emitte ... | 2009 | 19476282 |
| involvement of pi3 kinase and map kinase in igf-i- and insulin-induced oocyte maturation in cyprinus carpio. | previously, we observed that in vitro germinal vesicle breakdown (gvbd) in cyprinus carpio oocytes was induced by recombinant human insulin-like growth factor-i (igf-i) and bovine insulin (b-insulin) and this induction was steroid-independent. to investigate further the early signal transduction components involved in this process, the possible role of phosphatidylinositol 3-kinase (pi3 kinase) during oocyte maturation was examined. igf-i- and b-insulin-induced oocyte maturation was significantl ... | 2009 | 19482057 |
| physiological responses to mercury in feral carp populations inhabiting the low ebro river (ne spain), a historically contaminated site. | the low ebro river course (northeast spain) is historically affected by mercury pollution due to a chlor-alkali plant operating at the town of flix for more than a century. river sediments analysed during the last 10 years showed high mercury levels in the river section starting just downstream the factory and spanning some 90km, down to the river delta. the possible environmental impact was studied by a combination of field and laboratory studies. mercury concentrations in liver, kidney and mus ... | 2009 | 19482362 |
| trematode centrocestus formosanus infection and distribution in ornamental fishes in mexico. | the aim of this study was to determine the ornamental fish species affected by the metacercariae of the digenean trematode centrocestus formosanus and its distribution in 48 fish farms in morelos, central mexico. the parasite was found to form various numbers of branchial cysts in 11 of the 25 species analyzed. goldfish carassius auratus was the most commonly affected species; 20 of 30 farms were positive for this parasite, the fish showing severe clinical signs and having a high mortality rate. ... | 2009 | 19485122 |
| distribution of the introduced cyprinid herpesvirus 3 in a wild population of common carp, cyprinus carpio l. | cyprinid herpesvirus 3 (cyhv-3), which causes a lethal disease in common carp, cyprinus carpio l., and koi, c. carpio koi, first occurred in lake biwa, japan in 2004. to elucidate distribution of cyhv-3 in a wild common carp population, we conducted a pcr survey of cyhv-3 among such fish in lake biwa in 2006. only 6% (1/18) of the common carp smaller than 300 mm were positive with pcr, whereas 31% (18/58) of fish larger than 300 mm were positive. to evaluate their past exposure to cyhv-3 infecti ... | 2009 | 19500210 |
| [polyclonal antibody preparation and expression pattern analysis of carp]. | the present study aims at preparation of carp antibody and analysis of carp expression pattern. | 2009 | 19500504 |
| embolic implications of combined risk factors in patients with patent foramen ovale (the carpe criteria): consideration for primary prevention closure? | large patent foramen ovale (pfo), spontaneous right-to-left shunt, large atrial septal aneurysm (asa), coagulation abnormalities, and prominent eustachian valve (ev) have all been independently suggested as risk factors for recurrent stroke. we sought to retrospectively evaluate risk of stroke and impact of transcatheter pfo closure in patients with concurrent large pfo, spontaneous right-to-left shunt, large asa, coagulation abnormalities, and prominent ev. | 2009 | 19515082 |
| epizootic haematopoietic necrosis virus--an assessment of the likelihood of introduction and establishment in england and wales. | epizootic haematopoietic necrosis virus (ehnv) is an iridovirus that affects perch (perca fluviatilis) and rainbow trout (oncorhynchus mykiss). it emerged in australia in the 1980s and has not been discovered elsewhere. it causes a high level of mortality in perch resulting in steep population declines. the main possible routes of introduction of the virus to england and wales are the importation of infected live fish or carcasses. however, no trade in live susceptible species is permitted under ... | 2009 | 19515438 |
| tapeworm khawia sinensis: review of the introduction and subsequent decline of a pathogen of carp, cyprinus carpio. | the asian tapeworm khawia sinensis hsü, 1935 (cestoda: caryophyllidea) is a large-sized (body length up to 11.5 cm) monozoic (unsegmented) parasite of common carp (cyprinus carpio l.) that may cause mortality of young fish (fry). since the 1960s, this cestode successfully colonized a large part of europe, including the british isles, north america and japan. however, a review of published records provides evidence that the tapeworm k. sinensis, invasive parasite of carp, has become less common d ... | 2009 | 19520514 |
| common carp (cyprinus carpio) insulin-like growth factor binding protein-2 (igfbp-2): molecular cloning, expression profiles, and hormonal regulation in hepatocytes. | in the present study, we cloned igfbp-2 cdna from common carp (cyprinus carpio) liver. the 1879 bp full-length cdna encodes 274 amino acid residues containing a putative signal peptide of 22 residues. two igfbp-2 transcripts with estimated sizes of 2.2 and 1.5 kb have been detected with northern blot analysis in liver. relatively high levels of igfbp-2 mrna were observed in all regions of brain, liver, pituitary, ovary and testis. intermediate levels were observed in white muscle, thymus gland a ... | 2009 | 19523372 |
| mutations in the ankrd1 gene encoding carp are responsible for human dilated cardiomyopathy. | dilated cardiomyopathy (dcm) is familial in approximately 30% of cases, and mutations have been identified in several genes. however, in a majority of familial cases, the responsible genes are still to be discovered. the ankrd1 gene is over-expressed in heart failure in human and animal models. the encoded protein carp interacts with partners such as myopalladin or titin, previously shown to be involved in dcm. we hypothesized that mutations in ankrd1 could be responsible for dcm. | 2009 | 19525294 |
| biochemical changes and sensory assessment on tissues of carp (cyprinus carpio, linnaeus 1758) during sale conditions. | in this study, some biochemical changes of carp (cyprinus carpio, linnaeus 1758) tissues were investigated. studies have been carried out on carp which have regional economical importance. storage temperature and time are the most important factors that affect the quality of fish during sales. it was observed that the temperature varied between 9 and 12 degrees c in sale conditions. in addition, we assumed the arrival time of the fish at the fish market to be 0 (zero) h. biochemical analyses [ma ... | 2009 | 19533401 |
| identification and evaluation of a novel heterocyclic brominated flame retardant tris(2,3-dibromopropyl) isocyanurate in environmental matrices near a manufacturing plant in southern china. | a brominated flame retardant (bfr), hexabrominated heterocyclic tris-(2,3-dibromopropyl) isocyanurate (tbc), was identified,forthe first time, in the natural environment.the chemical was found in river water (2.33-163 ng/l), surface sediments (85.0 ng/g-6.03 microg/g dry weight (dw)), soils (19.6-672 ng/g dw), earthworm (9.75-78.8 ng/g dw), and carp samples (12.0-646 ng/g dw) from a factory-polluted area in southern china. it was found that tbc can strongly adsorb to organic material in sediment ... | 2009 | 19534117 |
| molecular characterization of complement factor i reveals constitutive expression in channel catfish. | the complement system in vertebrates plays a crucial role in immune defense via recognition and removal of pathogens. complement is tightly regulated by a group of both soluble and cell-associated proteins. complement factor i is a soluble serine protease that regulates multiple pathways in complement activation. in this work, a complement factor i transcript was isolated and sequenced from channel catfish (ictalurus punctatus) liver after screening expressed sequence tags. the full-length cdna ... | 2009 | 19540919 |
| high cgmp synthetic activity in carp cones. | cones show briefer light responses than rods and do not saturate even under very bright light. using purified rod and cone homogenates, we measured the activity of guanylate cyclase (gc), an enzyme responsible for cgmp synthesis and therefore recovery of a light response. the basal gc activity was 36 times higher in cones than in rods: it was mainly caused by higher expression levels of gc in cones (gc-c) than in rods (gc-r). with identification and quantification of gc-activating protein (gcap) ... | 2009 | 19556550 |
| genetic stability of progeny from an artificial allotetraploid carp using sperm from five fish species. | microsatellite markers and d-loop sequences of mtdna from a female allotetraploid parent carp and her progenies of generations 1 and 2 induced by sperm of five distant fish species were analyzed. eleven microsatellite markers were used to identify 48 alleles from the allotetraploid female. the same number of alleles (48) appeared in the first and second generations of the gynogenetic offspring, regardless of the source of the sperm used as an activator. the mtdna d-loop analysis was performed on ... | 2009 | 19565205 |