Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| toxicological profile of cereulide, the bacillus cereus emetic toxin, in functional assays with human, animal and bacterial cells. | some strains of the endospore-forming bacterium bacillus cereus produce a heat-stable ionophoric peptide, cereulide, of high human toxicity. we assessed cell toxicity of cereulide by measuring the toxicities of crude extracts of cereulide producing and non-producing strains of b. cereus, and of pure cereulide, using cells of human, animal and bacterial origins. hepatic cell lines and boar sperm, with cytotoxicity and sperm motility, respectively, as the end points, were inhibited by 1 nm of cere ... | 2007 | 17156808 |
| a gene encoding alanine racemase is involved in spore germination in bacillus thuringiensis. | alanine racemase is a major component of the exosporium of bacillus cereus spores. a gene homologous to that of alanine racemase (alra) was cloned from bacillus thuringiensis subsp. kurstaki, and rt-pcr showed that alra was transcribed only in the sporulating cells. disruption of alra did not affect the growth and sporulation of b. thuringiensis, but promoted l-alanine-induced spore germination. when the spore germination rate was measured by monitoring dpa release, complementation of the alra d ... | 2007 | 17165028 |
| intractable bacillus cereus bacteremia in a preterm neonate. | although often regarded as a contaminant, bacillus spp. have been implicated in serious systemic infections. the incidence of such infections is low with only a few cases reported in the literature. we describe the clinical course of early-onset bacillus cereus bacteremia in a preterm neonate who was successfully treated with vancomycin. | 2007 | 17166929 |
| ac2-dpd, the bis-(o)-acetylated derivative of 4,5-dihydroxy-2,3-pentanedione (dpd) is a convenient stable precursor of bacterial quorum sensing autoinducer ai-2. | ac2-dpd, the bis-(o)-acetylated derivative of 4,5-dihydroxy-2,3-pentanedione (dpd), was prepared both as a racemic mixture and in the optically active form found in naturally occurring dpd. it was shown to exhibit the same ability as dpd to induce bioluminescence in vibrio harveyi and beta-galactosidase activity in salmonella enterica typhimurium, both gram-negative bacteria. likewise, it was also shown to inhibit biofilm formation in gram-positive bacillus cereus. the most likely hypothesis is ... | 2007 | 17169556 |
| the properties of bacillus cereus hemolysin ii pores depend on environmental conditions. | hemolysin ii (hlyii), one of several cytolytic proteins encoded by the opportunistic human pathogen bacillus cereus, is a member of the family of oligomeric beta-barrel pore-forming toxins. this work has studied the pore-forming properties of hlyii using a number of biochemical and biophysical approaches. according to electron microscopy, hlyii protein interacts with liposomes to form ordered heptamer-like macromolecular assemblies with an inner pore diameter of 1.5-2 nm and an outer diameter of ... | 2007 | 17173854 |
| computer aided boar semen motility analysis for cereulide detection in different food matrices. | computer aided semen analysis (casa) study of the boar semen motility has been demonstrated to be an appropriate assay for detection of cereulide (bacillus cereus emetic toxin). application of the boar semen bio-assay to detect cereulide directly in foods requires investigation of potential interference of food components, preservatives and other microbial and chemical food contaminants with the bio-assay. current study provides evidence that none of included staphylococcus aureus enterotoxins a ... | 2007 | 17174428 |
| [cord blood transplantation after successful treatment of brain abscess caused by bacillus cereus in a patient with acute myeloid leukemia]. | central nervous system infection caused by bacillus cereus is a rare condition, which often progresses rapidly and is fatal in immunocompromised patients. a 54-year-old woman with acute myelogenous leukemia fell into a coma with high fever during severe neutropenia while undergoing chemotherapy. a blood culture demonstrated the presence of b. cereus and magnetic resonance imaging showed multiple abnormal lesions in her brain. the patient was treated with meropenem and vancomycin, and recovered f ... | 2006 | 17176890 |
| glucan and fructan production by sourdough weissella cibaria and lactobacillus plantarum. | after a large screening on sourdough lactic acid bacteria, exopolysaccharide (eps)-forming strains of weissella cibaria, lactobacillus plantarum, and pediococcus pentosaceus were selected. after 6 days of incubation at 30 degrees c, the synthesis of eps in mrs-based broth ranged from 5.54 to 7.88 mg ml-1. eps had an apparent molecular mass of ca. 104 da. as shown by carbohydrate consumption, the synthesis of eps was found from sucrose only. two types of homopolysaccharides were synthesized: gluc ... | 2006 | 17177514 |
| microbial composition in bioaerosols of a high-throughput chicken-slaughtering facility. | the microbial composition of the air in various areas of a high-throughput chicken-slaughtering facility was investigated. over a 4-mo period, 6 processing areas were sampled, and the influence of environmental factors was monitored. the highest counts of microorganisms were recorded in the initial stages of processing, comprising the receiving-killing and defeathering areas, whereas counts decreased toward the evisceration, air-chilling, packaging, and dispatch areas. maximum microbial counts w ... | 2007 | 17179429 |
| predictive modeling of bacillus cereus spores in farm tank milk during grazing and housing periods. | the shelf life of pasteurized dairy products depends partly on the concentration of bacillus cereus spores in raw milk. based on a translation of contamination pathways into chains of unit-operations, 2 simulation models were developed to quantitatively identify factors that have the greatest effect on the spore concentration in milk. in addition, the models can be used to determine the reduction in concentration that could be achieved via measures at the farm level. one model predicts the conce ... | 2007 | 17183096 |
| virulent spores of bacillus anthracis and other bacillus species deposited on solid surfaces have similar sensitivity to chemical decontaminants. | to compare the relative sensitivity of bacillus anthracis and spores of other bacillus spp. deposited on different solid surfaces to inactivation by liquid chemical disinfecting agents. | 2007 | 17184315 |
| the microbiological quality of hot water-washed broccoli florets and cut green beans. | to determine the effect of hot water washing on the microbiological quality of cut broccoli florets and trimmed green beans. | 2007 | 17184318 |
| survival and growth of foodborne pathogens during cooking and storage of oriental-style rice cakes. | fresh cooked rice cakes for retail sale are typically held at room temperature because refrigeration dramatically reduces their quality. room temperature, high water activity, and a ph of > 4.6 provided an environment conducive to pathogen growth. to date, no studies have been published regarding survival and growth of foodborne pathogens in fresh cooked rice cakes. this study was undertaken to investigate the effect of steam cooking on foodborne pathogens and their subsequent growth in five var ... | 2006 | 17186677 |
| antimicrobial and cytotoxic knotwood extracts and related pure compounds and their effects on food-associated microorganisms. | knotwood or bark extracts prepared from 30 species of hard and soft wood trees as well as selected pure compounds (lignans, stilbenes and flavonoids) were assayed for their antimicrobial activity against a battery of both gram positive and negative bacteria, yeasts, and filamentous fungi (bacillus cereus, staphylococcus aureus, listeria monocytogenes, lactobacillus plantarum, escherichia coli, salmonella infantis, pseudomonas fluorescens, candida albicans, saccharomyces cerevisiae, aspergillus f ... | 2007 | 17188387 |
| microbial inactivation by microwave radiation in the home environment. | the study reported here looked at the survival of microorganisms (heterotrophic plate counts, total coliforms, e. coli, and bacterial spores) in a consumer-type microwave oven. kitchen sponges, scrubbing pads, and syringes were experimentally contaminated with wastewater and subsequently exposed to microwave radiation. at 100 percent power level, it was found that the heterotrophic plate count (i.e., total bacterial count) of the wastewater was reduced by more that 99 percent within 1 to 2 minut ... | 2006 | 17190338 |
| four new compounds from the leaves of acer truncatum. | four new compounds, 3-(4-hydroxy-3,5-dimethoxyphenyl)propyl formate (1), 2,6-dimethoxy-4-[(1s)-3-methoxypropyl]phenol (2), (1r,2r)-4-[(3r)-3-hydroxybutyl]-3,3,5-trimethylcyclohex-4-ene-1,2-diol (3), and (1s,3r,3ar,6s,7s,9ar)-decahydro-1-(hydroxymethyl)-1,7-dimethyl-3a,7-methano-3ah-cyclopentacyclooctene (4) were isolated from the leaves of acer truncatum, together with twelve known compounds. their structures were elucidated on the basis of extensive spectroscopic techniques. the absolute config ... | 2006 | 17193311 |
| bacillus cereus strains fall into two clusters (one closely and one more distantly related) to bacillus anthracis according to amino acid substitutions in small acid-soluble proteins as determined by tandem mass spectrometry. | small acid-soluble proteins (sasps) are located in the core region of bacillus spores and have been previously demonstrated as reliable biomarkers for differentiating bacillus anthracis and bacillus cereus. using ms and ms-ms analysis of sasps further phylogenetic correlations among b. anthracis and b. cereus strains are described here. esi was demonstrated to be a more comprehensive method, allowing for the analysis of intact proteins in both ms and ms-ms mode, thus providing molecular weight ( ... | 2007 | 17197155 |
| the use of flow cytometry to study the germination of bacillus cereus endospores. | at present the study of endospore germination is conducted using microbiological methods which are slow and yield data based on the means of large heterogeneous populations. flow cytometry (fcm) offers the potential to rapidly quantify and identify germination and outgrowth events for large numbers of individual endospores. | 2007 | 17200957 |
| ceramidase enhances phospholipase c-induced hemolysis by pseudomonas aeruginosa. | we previously reported the purification, molecular cloning, and characterization of a neutral ceramidase from pseudomonas aeruginosa strain an17 (okino, n., tani, m., imayama, s., and ito, m. (1998) j. biol. chem. 273, 14368-14373; okino, n., ichinose, s., omori, a., imayama, s., nakamura, t., and ito, m. (1999) j. biol. chem. 274, 36616-36622). interestingly, the gene encoding the enzyme is adjacent to that encoding hemolytic phospholipase c (plch) in the genome of pseudomonas aeruginosa, which ... | 2007 | 17202150 |
| fatal pneumonia among metalworkers due to inhalation exposure to bacillus cereus containing bacillus anthracis toxin genes. | bacillus cereus pneumonia is unusual in nonimmunocompromised hosts. we describe fatal cases in 2 metalworkers and the associated investigation. anthrax toxin genes were identified in b. cereus isolates from both patients using polymerase chain reaction. finding anthrax toxin genes in non-bacillus anthracis isolates has, to our knowledge, only been reported once previously. | 2007 | 17205450 |
| air-liquid interface biofilms of bacillus cereus: formation, sporulation, and dispersion. | biofilm formation by bacillus cereus was assessed using 56 strains of b. cereus, including the two sequenced strains, atcc 14579 and atcc 10987. biofilm production in microtiter plates was found to be strongly dependent on incubation time, temperature, and medium, as well as the strain used, with some strains showing biofilm formation within 24 h and subsequent dispersion within the next 24 h. a selection of strains was used for quantitative analysis of biofilm formation on stainless steel coupo ... | 2007 | 17209076 |
| [survival of gram-positive spore-forming bacteria including bacillus cereus after hand washing using alcohol-based handrub]. | hand washing is the most fundamental method for preventing infection. currently, hand washing with an alcohol-based handrub is the international gold standard method. however, in our study we found many samples of ineffective hand washing using an alcohol-based handrub. the rates of ineffective samples were 10.4% (5/48) in 2004 and 34.3% (12/35) in 2005. we examined the morphology by gram staining and biochemical properties of the bacteria which remained after hand washing in 2005. their colonie ... | 2006 | 17209516 |
| chitin-supplemented foliar application of chitinolytic bacillus cereus reduces severity of botrytis gray mold disease in chickpea under controlled conditions. | to identify and evaluate chitinolytic bacteria for control of botrytis gray mold (bgm), a devastating disease in chickpea. methods and results: two antifungal bacterial isolates, chitinolytic bacillus cereus crs 7 and nonchitinolytic pseudomonas fluorescens crs 31, from the rhizosphere of chickpea, were applied as a prophylactic foliar spray and evaluated for control of bgm. in a controlled environment, the two isolates reduced the severity of bgm on the susceptible cv. jg 62 to 6.0 and 5.6, res ... | 2007 | 17209822 |
| dimer structure of an interfacially impaired phosphatidylinositol-specific phospholipase c. | the crystal structure of the w47a/w242a mutant of phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus thuringiensis has been solved to 1.8a resolution. the w47a/w242a mutant is an interfacially challenged enzyme, and it has been proposed that one or both tryptophan side chains serve as membrane interfacial anchors (feng, j., wehbi, h., and roberts, m. f. (2002) j. biol. chem. 277, 19867-19875). the crystal structure supports this hypothesis. relative to the crystal structure of ... | 2007 | 17213187 |
| microbiological investigation and nutritional evaluation of selected fast food meat. | the study was designed into two parts: the first part was to determine individual attitudes and beliefs toward fast food in general. one hundred individuals (15-45 yrs old) were involved in this study (50 males and 50 females). the second part of the study was carried out to evaluate microbiological contamination and nutritive value of the selected fast food meat (hardee's fried burger, saudi-american burger, kentucky fried chicken, al-baik broast chicken and shawerma beef). the results indicate ... | 1995 | 17214203 |
| kinetics of plasmid transfer among bacillus cereus group strains within lepidopteran larvae. | the cry toxin encoding plasmid pht73 was transferred from bacillus thuringiensis subspecies kurstaki kt0 to six b. cereus group strains in three lepidopteran (spodoptera exigua, plutella xyllostella and helicoverpa armigera) larvae by conjugation. the conjugation kinetics of the plasmid was precisely studied during the larval infection using a new protocol. the infections were performed with both vegetative and sporulated strains. however, larval death only occurred when infections were made wit ... | 2007 | 17216168 |
| the inhibitory effect of garlic (allium sativum) on growth of some microorganisms. | the aim of the present study was to compare between the anti-microbial activity of fresh garlic bulbs and that of its water extract against the potent food pathogens staphylococcus aureus, esherichia coil, salmonella typhi and bacillus cereus. garlic segments with different concentrations (1%, 5%, and 10%) were used. another 100 gms of garlic segments were weighed and mixed with 100 ml distilled water to prepare water extract of garlic, then 1000, 500 and 100 microg/ml were prepared. these conce ... | 2003 | 17219900 |
| detection of vibrio cholerae by real-time nucleic acid sequence-based amplification. | a multitarget molecular beacon-based real-time nucleic acid sequence-based amplification (nasba) assay for the specific detection of vibrio cholerae has been developed. the genes encoding the cholera toxin (ctxa), the toxin-coregulated pilus (tcpa; colonization factor), the ctxa toxin regulator (toxr), hemolysin (hlya), and the 60-kda chaperonin product (groel) were selected as target sequences for detection. the beacons for the five different genetic targets were evaluated by serial dilution of ... | 2007 | 17220262 |
| identification of three zwittermicin a biosynthesis-related genes from bacillus thuringiensis subsp. kurstaki strain ybt-1520. | zwittermicin a (zwa) is a novel, broad-spectrum linear aminopolyol antibiotic produced by some bacillus cereus and bacillus thuringiensis. however, only part of its biosynthesis cluster has been identified and characterized from b. cereus uw85. to better understand the biosynthesis cluster of zwa, a bacterial artificial chromosome (bac) library of b. thuringiensis subsp. kurstaki strain ybt-1520, a zwa-producing strain, was constructed. two bac clones, 1f8 and 5e2, were obtained by pcr, which ov ... | 2007 | 17225146 |
| low molecular weight chitosans--preparation with the aid of pronase, characterization and their bactericidal activity towards bacillus cereus and escherichia coli. | the homogeneous low molecular weight chitosans (lmwc) of molecular weight 9.5-8.5 kda, obtained by pronase catalyzed non-specific depolymerization (at ph 3.5, 37 degrees c) of chitosan showed lyses of bacillus cereus and escherichia coli more efficiently (100%) than native chitosan (<50%). ir and (1)h-nmr data showed decrease in the degree of acetylation (14-19%) in lmwc compared to native chitosan ( approximately 26%). minimum inhibitory concentration of lmwc towards 10(6) cfu ml(-1) of b. cere ... | 2007 | 17240531 |
| difference between the spore sizes of bacillus anthracis and other bacillus species. | to determine the size distribution of the spores of bacillus anthracis, and compare its size with other bacillus species grown and sporulated under similar conditions. | 2007 | 17241334 |
| genotypic and phenotypic diversity of lactococcus lactis isolates from batzos, a greek pdo raw goat milk cheese. | the genotypic and phenotypic variability of 40 lactococcus lactis isolates obtained from three cheese-making trials of batzos cheese made one in each, winter, spring and summer was investigated. rapd-pcr, plasmid profiling and pfge were used to study the genetic variability and distinguish closely related isolates. results showed a high degree of heterogeneity among strains. according to pfge data, all strains except one were clustered together (at a similarity level of approximately 50%) with t ... | 2007 | 17241681 |
| hqno-sensitive nadh:quinone oxidoreductase of bacillus cereus kctc 3674. | the enzymatic properties of nadh:quinone oxidoreductase were examined in triton x-100 extracts of bacillus cereus membranes by using the artificial electron acceptors ubiquinone-1 and menadione. membranes were prepared from b. cereus kctc 3674 grown aerobically on a complex medium and oxidized with nadh exclusively, whereas deamino-nadh was determined to be poorly oxidized. the nadh oxidase activity was lost completely by solubilization of the membranes with triton x-100. however, by using the a ... | 2007 | 17244482 |
| asymmetric synthesis of diastereomeric diaminoheptanetetraols. a proposal for the configuration of (+)-zwittermicin a. | [structure: see text] a proposed absolute configuration for the 7 stereocenters in (+)-zwittermicin a is described based on asymmetric synthesis of six diastereomeric 2,6-diamino-1,3,5,7-heptanetetraols corresponding to the c9-c15 segment, pairwise 13c nmr chemical shift difference analysis of the models with the natural product, interpretation of enantiospecificity of the serine loading domain of the zwittermicin a biosynthetic gene cluster, and degradation of the natural product. | 2007 | 17249781 |
| low molecular weight chitosan--preparation with the aid of pepsin, characterization, and its bactericidal activity. | pepsin (ec 3.4.4.1) from porcine stomach mucosa caused depolymerization of a chitosan sample (a copolymer of glucosamine and n-acetylglucosamine linked by beta-1-4-glycosidic bonds). n-terminal sequence and zymogram analyses confirmed dual (proteolytic and chitosanolytic) activities of pepsin. optimum depolymerization occurred at ph 5.0 and 45 degrees c with an activity of 4.98 u. low molecular weight chitosan (lmwc), the major depolymerization product, was obtained in a yield of 75-82%, the deg ... | 2007 | 17256986 |
| the redox regulator fnr is required for fermentative growth and enterotoxin synthesis in bacillus cereus f4430/73. | glucose-grown cells of bacillus cereus respond to anaerobiosis and low extracellular oxidoreduction potentials (orp), notably by enhancing enterotoxin production. this response involves the resde two-component system. we searched the b. cereus genome for other redox response regulators potentially involved in this adaptive process, and we identified one gene encoding a protein predicted to have an amino acid sequence 58% identical (80% similar) to that of the bacillus subtilis fnr redox regulato ... | 2007 | 17259311 |
| diagnostic real-time pcr assays for the detection of emetic bacillus cereus strains in foods and recent food-borne outbreaks. | cereulide-producing bacillus cereus can cause an emetic type of food-borne disease that mimics the symptoms provoked by staphylococcus aureus. based on the recently discovered genetic background for cereulide formation, a novel 5' nuclease (taqman) real-time pcr assay was developed to provide a rapid and sensitive method for the specific detection of emetic b. cereus in food. the taqman assay includes an internal amplification control and primers and a probe designed to target a highly specific ... | 2007 | 17259359 |
| of spore opsonization and passive protection against anthrax. | 2007 | 17259600 | |
| method of measuring bacillus anthracis spores in the presence of copious amounts of bacillus thuringiensis and bacillus cereus. | a sensitive and reliable method for the detection of bacillus anthracis (ba; sterne strain 7702) spores in presence of large amounts of bacillus thuringiensis (bt) and bacillus cereus (bc) is presented based on a novel pzt-anchored piezoelectric excited millimeter-sized cantilever (papemc) sensor with a sensing area of 1.5 mm2. antibody (anti-ba) specific to ba spores was immobilized on the sensing area and exposed to various samples of ba, bt, and bc containing the same concentration of ba at 3 ... | 2007 | 17263347 |
| role of bacterial and host factors in infectious endophthalmitis. | endophthalmitis is a frequent blinding complication of globe-penetrating injury and ocular surgery. the outcome of this intraocular infection depends both on the organism involved and management of the ensuing inflammation. the role of various toxins and bacterial factors in the pathogenesis of this infection is beginning to be delineated, but appears to be organism specific. because of the immune-privileged environment of the eye, principles important in the resolution of infection at extraocul ... | 2007 | 17264502 |
| titanium dioxide/uv photocatalytic disinfection in fresh carrots. | increased occurrences of fresh produce-related outbreaks of foodborne illness have focused attention on effective washing processes for fruits and vegetables. a titanium dioxide (tio2) photocatalytic reaction under uv radiation provides a high rate of disinfection. the photo-killing effects of tio2 on bacteria in liquid cultures under experimental conditions have been widely studied. however, the disinfection effects of the tio2 photocatalytic reaction on fresh vegetables during a washing proces ... | 2007 | 17265866 |
| use of high-affinity cell wall-binding domains of bacteriophage endolysins for immobilization and separation of bacterial cells. | immobilization and magnetic separation for specific enrichment of microbial cells, such as the pathogen listeria monocytogenes, depends on the availability of suitable affinity molecules. we report here a novel concept for the immobilization and separation of bacterial cells by replacing antibodies with cell wall-binding domains (cbds) of bacteriophage-encoded peptidoglycan hydrolases (endolysins). these polypeptide modules very specifically recognize and bind to ligands on the gram-positive cel ... | 2007 | 17277212 |
| structural studies of thymidine kinases from bacillus anthracis and bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding. | thymidine kinase (tk) is the key enzyme in salvaging thymidine to produce thymidine monophosphate. owing to its ability to phosphorylate nucleoside analogue prodrugs, tk has gained attention as a rate-limiting drug activator. we describe the structures of two bacterial tks, one from the pathogen bacillus anthracis in complex with the substrate dt, and the second from the food-poison-associated bacillus cereus in complex with the feedback inhibitor dttp. interestingly, in contrast with previous s ... | 2007 | 17288553 |
| food poisoning potential of bacillus cereus strains from norwegian dairies. | characteristics concerning diarrhoeal potential were investigated in b. cereus dairy strains. the thirty-nine strains, isolated from whipping cream, were tested for cytotoxicity after culturing at human body temperature as well as 25 degrees c and 32 degrees c. at 37 degrees c, none of the strains were highly cytotoxic. this observation suggests that those strains should be considered to pose a minor risk with regard to diarrhoeal food poisoning. however, some strains were moderately or highly c ... | 2007 | 17292990 |
| degradation of n-acyl-l-homoserine lactones by bacillus cereus in culture media and pork extract. | degradation of the quorum-sensing signal molecule n-acyl-l-homoserine lactone (ahl) in cocultures was verified with bacillus cereus and yersinia enterocolitica in culture medium and in pork extract. results showed evidence of microbial interaction when the ahl-degrading bacterium and ahl-producing bacterium were cocultured in a food-simulating condition. | 2007 | 17293532 |
| changes in numbers and kinds of bacteria during a chickpea submerged fermentation used as a leavening agent for bread production. | the microflora developed during a submerged fermentation of coarsely ground chickpea (cicer arietinum l.) in water (primary starter) and during raising a dough from wheat flour (adapted starter) was studied. in the fermenting liquid, only populations of bacillus and clostridium developed. bacilli increased their loads significantly (p<0.05) during fermentation for 8-12 h and then remained constant. clostridia developed (p<0.05) subsequently to levels of 10(7) cfu/ml at 18 h, when the ph of the f ... | 2007 | 17300848 |
| group ii intron in bacillus cereus has an unusual 3' extension and splices 56 nucleotides downstream of the predicted site. | all group ii introns known to date fold into six functional domains. however, we recently identified an intron in bacillus cereus atcc 10987, b.c.i4, that splices 56 nt downstream of the expected 3' splice site in vivo (tourasse et al. 2005, j. bacteriol., 187, 5437-5451). in this study, we confirmed by ribonuclease protection assay that the 56-bp segment is part of the intron rna molecule, and computational prediction suggests that it might form a stable stem-loop structure downstream of domain ... | 2007 | 17301069 |
| [screening and real-time quantitative detection for genomic signatures of bacillus anthracis]. | a total amount of 117 candidate chromosomal sequences were screened for the genomic signatures of bacillus anthracis by a 2-step approach. out of them, 19 genomic signatures sequences were selected, among which, 6 genomic signatures were competent as the target sequence of taqman real-time pcr method. with the most significant alignment and specificity, fragment c04, together with paga gene and capb gene from virulence plasmids px01 and px02, was selected to establish a taqman real-time pcr assa ... | 2006 | 17302151 |
| virulence of bacillus cereus: a multivariate analysis. | biological activity and presence of dna sequences related to virulence genes were studied in 21 strains of the bacillus cereus group. the activity of spent culture supernatants and the effect of infection by vegetative bacterial cells were assessed on cultured human enterocytes (caco-2 cells). the effect of extracellular factors on the detachment, necrosis and mitochondrial dehydrogenase activity of cultured human enterocytes was studied. hemolytic activity on rabbit red blood cells was also eva ... | 2007 | 17303280 |
| antibacterial effect of some essential oils administered alone or in combination with norfloxacin. | the objective of the present study was that of verifying a possible synergistic antibacterial effect between pelargonium graveolens [lis-balchin, m., deans, s.g., hart, s., 1996. bioactive geranium oils from different commercial sources. j. essential oil res. 8, 281-290.] essential oil (and its main components) and norfloxacin antibiotic. as a first step growth inhibition by some types of essential oils was assessed in five microbial species. the antimicrobial effects of p. graveolens oil, as we ... | 2007 | 17303397 |
| role of zinc content on the catalytic efficiency of b1 metallo beta-lactamases. | metallo beta-lactamases (mbetal) are enzymes naturally evolved by bacterial strains under the evolutionary pressure of beta-lactam antibiotic clinical use. they have a broad substrate spectrum and are resistant to all the clinically useful inhibitors, representing a potential risk of infection if massively disseminated. the mbetal scaffold is designed to accommodate one or two zinc ions able to activate a nucleophilic hydroxide for the hydrolysis of the beta-lactam ring. the role of zinc content ... | 2007 | 17305336 |
| characteristics of bacteriocin-like inhibitory substances from dochi-isolated enterococcus faecium d081821 and d081833. | to characterize bacteriocin-like inhibitory substances (blis) from two dochi-isolated enterococcus faecium. | 2007 | 17309511 |
| characterization of mesophilic bacilli in faeces of feedlot cattle. | to determine the identity and composition of mesophilic bacillus spp. in faeces sampled from feedlot cattle. | 2007 | 17309638 |
| biomedical evaluation of two sympatric lemur species (propithecus verreauxi deckeni and eulemur fulvus rufus) in tsiombokibo classified forest, madagascar. | complete medical examinations were performed on 20 wild decken's sifaka (propithecus verreauxi deckeni) and 20 wild red-fronted brown lemurs (eulemurfulvus rufus) from western madagascar. each animal received a complete physical examination, and weight, body temperature, heart rate, and respiratory rate were recorded and ectoparasites collected. blood samples were collected for complete blood cell count, differential white blood cell count, hemoparasite examination, serum biochemical profile, fa ... | 2005 | 17312713 |
| a new phylogenetic cluster of cereulide-producing bacillus cereus strains. | phenotypic and molecular studies have established that cereulide-producing strains of bacillus cereus are a distinct and probably recently emerged clone within the bacillus population. we analyzed a set of b. cereus strains, both cereulide producers and nonproducers, by multilocus sequence typing. consistent with earlier reports, nonproducers demonstrated high heterogeneity. most cereulide-producing strains and all flagellar antigen type h1 strains were allocated to the known sequence type of ex ... | 2007 | 17314223 |
| nanowire labeled direct-charge transfer biosensor for detecting bacillus species. | a direct-charge transfer (dct) biosensor was developed for the detection of the foodborne pathogen, bacillus cereus. the biosensor was fabricated using antibodies as the sensing element and polyaniline nanowire as the molecular electrical transducer. the sensor design consisted of four membrane pads, namely, sample application, conjugate, capture and absorption pads. two sets of polyclonal antibodies, secondary antibodies conjugated with polyaniline nanowires and capture antibodies were applied ... | 2007 | 17320373 |
| structural studies examining the substrate specificity profiles of pc-plc(bc) protein variants. | the phosphatidylcholine preferring phospholipase c from bacillus cereus (pc-plc(bc)) catalyzes the hydrolysis of phospholipids in the following order of preference: phosphatidylcholine (pc)>phosphatidylethanolamine (pe)>phosphatidylserine (ps). in previous work, mutagenic, kinetic, and crystallographic experiments suggested that varying the amino acids at the 4th, 56th, and 66th positions had a significant influence upon the substrate specificity profile of pc-plc(bc). here, we report the crysta ... | 2007 | 17324372 |
| quantification of magnetic susceptibility in several strains of bacillus spores: implications for separation and detection. | three strains of bacillus: bacillus atrophaeus (formally bacillus globigii), bacillus thuringiensis, and bacillus cereus were tested for their intrinsic magnetic susceptibility. all three strains when sporulated demonstrated significant magnetic susceptibility using an instrument referred to as cell tracking velocimetry. energy dispersive spectroscopy also confirmed the presence of paramagnetic elements, fe and mn, in the spore form of the bacteria. it was demonstrated that this magnetic suscept ... | 2007 | 17335063 |
| the use of clinical profiles in the investigation of foodborne outbreaks in restaurants: united states, 1982-1997. | improving the efficiency of outbreak investigation in restaurants is critical to reducing outbreak-associated illness and improving prevention strategies. because clinical characteristics of outbreaks are usually available before results of laboratory testing, we examined their use for determining contributing factors in outbreaks caused by restaurants. all confirmed foodborne outbreaks reported to the centers for disease control and prevention (cdc) from 1982 to 1997 were reviewed. clinical pro ... | 2008 | 17335632 |
| [a case of fatal neonatal bacillus cereus meningitis]. | a fatal case of meningoencephalitis caused by bacillus cereus, an uncommon but potential pathogen, resistant to most beta-lactam antibiotics, is described in a 28-day old premature neonate. difficulties for clinical diagnosis and treatment are discussed. a review of the literature (26 published cases) is given. early diagnosis of neonatal b. cereus infection is crucial as it leads to a standard treatment including vancomycin. | 2007 | 17337168 |
| [study on serum and urine concentrations of erythromycin succinate of pyrrolidine-methyl-tetracycline]. | the serum and urine antibiotic levels in a group of 25 patients were determined after a single administration of a new antibiotic salt: erythromycin succinate of pyrrolidine-methyl-tetracycline. some subjects were treated orally and other intramuscularly. the results obtained showed that this drug gives, both orally and intramuscularly, efficient serum and urine concentrations of tetracycline and erythromycin. | 1968 | 17340794 |
| presence and significance of bacillus cereus in dehydrated potato products. | dehydrated potato contains bacillus cereus at a prevalences of 10 to 40% and at numbers usually less than 10(3) cfu g(-1). b. cereus in dehydrated potato is likely to be present as spores that are able to survive drying of the raw vegetable and may represent a significant inoculum in the reconstituted (rehydrated) product where conditions favor germination of, and outgrowth from, spores. holding rehydrated mashed potato alone, or as part of another product (e.g., potato-topped pie), at temperatu ... | 2007 | 17340893 |
| two hlyiir dimers bind to a long perfect inverted repeat in the operator of the hemolysin ii gene from bacillus cereus. | hlyiir is a negative transcriptional regulator of hemolysin ii gene from b. cereus. it binds to a long dna perfect inverted repeat (44bp) located upstream the hlyii gene. here we show that hlyiir is dimeric in solution and in bacterial cells. no protein-protein interactions between dimers and no significant modification of target dna conformation upon complex formation were observed. two hlyiir dimers were found to bind to native operator independently with kd level in the nanomolar range. the m ... | 2007 | 17346714 |
| chromosomal-gene-mediated inhibition of intestinal and foodborne pathogens by lactobacillus acidophilus aa11. | approximately 63 strains of lactobacillus acidophilus were isolated from egyptian home-made cheese and examined for production of antagonism. only eight strains demonstrated inhibitory activity against spoilage microorganisms (i.e. staphylococcus aureus and bacillus cereus) and pathogens (i.e. e. coli, salmonella sp. and shigella sp.). lactobacillus acidophilus aa11 produced a more antimicrobial activity with a wide range of inhibition. the agent aa11 was sensitive to proteolytic enzymes and ret ... | 2006 | 17357571 |
| microbiological detection of 10 quinolone antibiotic residues and its application to artificially contaminated poultry samples. | to assess if microbiological inhibition tests for detection of antibiotic residues are suitable for routine screening for quinolone residues, the limit of detection (lod) of 10 different quinolones and fluoroquinolones was determined. two media were tested, one at ph 6 and the other at ph 8, each seeded with one of the following test strains: bacillus subtilis, escherichia coli or bacillus cereus. lods of the 10 substances were highest on plates seeded with b. cereus, intended for selective dete ... | 2007 | 17364926 |
| antimicrobial activity and pollen composition of honey samples collected from different provinces in turkey. | the antibacterial activity of honey samples from different sources were collected and investigated against bacillus cereus, staphylococcus aureus atcc 25923, pseudomonas aeruginosa atcc 27853, klebsiella pneumoniae atcc 27736, morganella morganii, micrococcus luteus nrrl b-4375, escherichia coli atcc 35218, and candida albicans. pathogens exhibited different sensitivities towards the honey samples. the results showed that majority of the honey samples (75%) generally inhibitied the bacteria test ... | 2007 | 17365707 |
| bacillus cereus strain 10-l-2 produces two arylamine n-acetyltransferases that transform 4-phenylenediamine into 4-aminoacetanilide. | a bacterium, strain 10-l-2, that was isolated from soil and identified as bacillus cereus grew well on medium containing 4-phenylenediamine and polypepton. strain 10-l-2 converted a wide variety of anilines, including 4-phenylenediamine, to their corresponding acetanilides. growing cells acetylated a single amino group of 4-phenylenediamine to form 4-aminoacetanilide with a 97% molar yield, as shown by mass spectrometry and hplc. cell extracts exhibited arylamine n-acetyltransferase (nat) activi ... | 2007 | 17368397 |
| inactivation of foodborne pathogens using a one atmosphere uniform glow discharge plasma. | this study was conducted to determine the efficacy of a one atmosphere uniform glow discharge plasma (oaugdp) for inactivation of foodborne pathogens and to evaluate the influence of growth temperature, ph, and culture age on their inactivation. escherichia coli o157:h7, listeria monocytogenes, staphylococcus aureus, bacillus cereus, salmonella enteritidis, vibrio parahaemolyticus, yersinia enterocolitica, and shigella flexneri were evaluated. three-strain mixtures of each bacterium were inocula ... | 2007 | 17378708 |
| a streamlined metabolic pathway for the biosynthesis of moenomycin a. | moenomycin a (mma) is a member of the phosphoglycolipid family of antibiotics, which are the only natural products known to directly target the extracellular peptidoglycan glycosyltransferases involved in bacterial cell wall biosynthesis. the structural and biological uniqueness of mma make it an attractive starting point for the development of new antibacterial drugs. in order both to elucidate the biosynthesis of this unusual compound and to develop tools to manipulate its structure, we have i ... | 2007 | 17379141 |
| culturable bacteria present in the fluid of the hooded-pitcher plant sarracenia minor based on 16s rdna gene sequence data. | the culturable microbial community within the pitcher fluid of 93 sarracenia minor carnivorous plants was examined over a 2-year study. many aspects of the plant/bacterial/insect interaction within the pitcher fluid are minimally understood because the bacterial taxa present in these pitchers have not been identified. thirteen isolates were characterized by 16s rdna sequencing and subsequent phylogenetic analysis. the proteobacteria were the most abundant taxa and included representatives from s ... | 2007 | 17380356 |
| antimicrobial activity of bacillus subtilis and bacillus pumilus during the fermentation of african locust bean (parkia biglobosa) for soumbala production. | to examine predominant isolates of bacillus subtilis and b. pumilus isolated from soumbala for their antimicrobial activity against indicator microorganisms as micrococcus luteus, staphyloccocus aureus, bacillus cereus, enterococus facium, listeria monocytogenes, escherichia coli, salmonella typhimurium, shigella dysenteriae, yersinia enterocolitica, aspergillus ochraceus and penicillium roqueforti. | 2007 | 17381739 |
| characterization of a bacteriocin, thermophilin 1277, produced by streptococcus thermophilus sbt1277. | to assess the inhibitory activity and the influence of culture condition on the growth and bacteriocin, thermophilin 1277, production by streptococcus thermophilus sbt1277. | 2007 | 17381740 |
| effect of bovicin hc5 on growth and spore germination of bacillus cereus and bacillus thuringiensis isolated from spoiled mango pulp. | to use bovicin hc5 to inhibit predominant bacteria isolated from spoiled mango pulp. | 2007 | 17381743 |
| proteomic analysis of bacillus cereus growing in liquid soil organic matter. | bacillus cereus is believed to be a soil bacterium, but studied solely in laboratory culture media. the aim of this study was to assess the physiology of b. cereus growing on soil organic matter by a proteomic approach. cells were cultured to mid-exponential phase in soil extracted solubilized organic matter (sesom), which mimics the nutrient composition of soil, and in luria-bertani broth as control. silver staining of the two-dimensional gels revealed 234 proteins spots up-regulated when cells ... | 2007 | 17391366 |
| structural insight into repair of alkylated dna by a new superfamily of dna glycosylases comprising heat-like repeats. | 3-methyladenine dna glycosylases initiate repair of cytotoxic and promutagenic alkylated bases in dna. we demonstrate by comparative modelling that bacillus cereus alkd belongs to a new, fifth, structural superfamily of dna glycosylases with an alpha-alpha superhelix fold comprising six heat-like repeats. the structure reveals a wide, positively charged groove, including a putative base recognition pocket. this groove appears to be suitable for the accommodation of double-stranded dna with a fli ... | 2007 | 17395642 |
| insecticidal bacteria isolated from predatory larvae of the antlion species myrmeleon bore (neuroptera: myrmeleontidae). | various bacterial species were isolated from the crop (digestive organ) of the antlion species myrmeleon bore and tested for their insecticidal activity against caterpillars by injection. sixty-eight isolates from the antlion crop were grouped into twenty-four species based on homologies of 16s rrna gene sequences and biochemical properties. isolated bacillus cereus, bacillus sphaericus, morganella morganii, serratia marcescens and a klebsiella species killed 80% or more cutworms when injected a ... | 2007 | 17399737 |
| differentiation of bacillus anthracis, b. cereus, and b. thuringiensis by using pulsed-field gel electrophoresis. | a pulsed-field gel electrophoresis (pfge) method was developed for discriminating bacillus anthracis from b. cereus and b. thuringiensis. a worldwide collection of 25 b. anthracis isolates showed high-profile homology, and these isolates were unambiguously distinguished from b. cereus and b. thuringiensis isolates by cluster analysis of the whole-genome macrorestriction enzyme digestion patterns generated by noti. | 2007 | 17400781 |
| properties of bacterial corrosion of stainless steel and its inhibition by protamine coating. | we investigated characteristics of the corrosion of stainless steel specimens by bacteria and the effects of using antimicrobial coating on the surface for inhibiting corrosion. bacillus sp. 2-a and staphylococcus sp. 2-1 cells adhered tightly to a stainless steel sus304 specimen, formed a microcolony or biofilm, and had highly corrosive activities. microbially influenced corrosion (mc) was observed under or around adhering cells. however, dead cells were markedly less active than viable cells n ... | 2007 | 17408005 |
| germination of bacillus cereus spores adhered to stainless steel. | adhered spores of bacillus cereus represent a significant part of the surface-derived contamination in processing equipment used in the dairy industry. as germinated spores lose their resistance capacities instantaneously, efficient germination prior to a cleaning in place treatment could aid to the disinfecting effect of such a treatment. therefore, spores of b. cereus atcc 14579 and that of the environmental isolate b. cereus cmcc 3328 were assessed for their germination behaviour when adhered ... | 2007 | 17408793 |
| identification of the sigmab regulon of bacillus cereus and conservation of sigmab-regulated genes in low-gc-content gram-positive bacteria. | the alternative sigma factor sigma(b) has an important role in the acquisition of stress resistance in many gram-positive bacteria, including the food-borne pathogen bacillus cereus. here, we describe the identification of the set of sigma(b)-regulated genes in b. cereus by dna microarray analysis of the transcriptome upon a mild heat shock. twenty-four genes could be identified as being sigma(b) dependent as witnessed by (i) significantly lower expression levels of these genes in mutants with a ... | 2007 | 17416654 |
| detection of enterotoxic bacillus cereus producing hemolytic and non hemolytic enterotoxins by pcr test. | nine strains belonging to bacillus cereus group has been isolated from food and environmental samples. their taxonomic position was confirmed by rflp analysis of 16s rrna gene digested with taqi. the detection of dna sequences encoding the hemolysin bl complex and enterotoxin nhe, was studied in bacillus sp. isolates. set of primers was used to amplify fragment of hbld gene by pcr. for the detection of nheb gene a new primer set was developed which allowed to amplify 273 bp fragment from wide nu ... | 2006 | 17419288 |
| asp-120 locates zn2 for optimal metallo-beta-lactamase activity. | metallo-beta-lactamases are zinc-dependent hydrolases that inactivate beta-lactam antibiotics, rendering bacteria resistant to them. asp-120 is fully conserved in all metallo-beta-lactamases and is central to catalysis. several roles have been proposed for asp-120, but so far there is no agreed consensus. we generated four site-specifically substituted variants of the enzyme bcii from bacillus cereus as follows: d120n, d120e, d120q, and d120s. replacement of asp-120 by other residues with very d ... | 2007 | 17426028 |
| multiplexed identification of blood-borne bacterial pathogens by use of a novel 16s rrna gene pcr-ligase detection reaction-capillary electrophoresis assay. | we have developed a novel high-throughput pcr-ligase detection reaction-capillary electrophoresis (pcr-ldr-ce) assay for the multiplexed identification of 20 blood-borne pathogens (staphylococcus epidermidis, staphylococcus aureus, bacillus cereus, enterococcus faecalis, enterococcus faecium, listeria monocytogenes, streptococcus pneumoniae, streptococcus pyogenes, streptococcus agalactiae, escherichia coli, klebsiella pneumoniae, haemophilus influenzae, pseudomonas aeruginosa, acinetobacter bau ... | 2007 | 17428930 |
| extending the bacillus cereus group genomics to putative food-borne pathogens of different toxicity. | the bacillus cereus group represents sporulating soil bacteria containing pathogenic strains which may cause diarrheic or emetic food poisoning outbreaks. multiple locus sequence typing revealed a presence in natural samples of these bacteria of about 30 clonal complexes. application of genomic methods to this group was however biased due to the major interest for representatives closely related to bacillus anthracis. albeit the most important food-borne pathogens were not yet defined, existing ... | 2008 | 17434157 |
| differentiation between bacillus thuringiensis strains by gyrb pcr-sau3ai fingerprinting. | gyrb dna fragments of seven bacillus thuringiensis local collection family representatives were amplified by pcr and sequenced. several differences in their corresponding sequences were evidenced. both in silico and in vitro restriction maps of gyrb sequences and fragments respectively confirmed that ecori and sau3ai could be used to differentiate between b. thuringiensis strains. however, the phylogeny analysis showed that only the gyrb pcr-sau3ai allows a strains classification that correlates ... | 2007 | 17435283 |
| [cloning and characterization of germ gene involved in germination in bacillus thuringiensis]. | in bacilli, germ is a very conservative gene. primers were designed according to the germ gene sequence of bacillus cereus, and a 640bp dna fragment was obtained from bacillus thuringiensis subsp. kustaki 1. 175 by pcr. using this fragment as a probe, a 4.5kb dna fragment was cloned from the partial dna library of bacillus thuringiensis subsp. kustaki 1.175. sequence analysis showed that the fragment contains one complete open reading frame (orf) that encodes a 349-amino acid (aa) protein, which ... | 2007 | 17436617 |
| the in vitro antibacterial activity of dietary spice and medicinal herb extracts. | the in vitro antibacterial activities of a total of 46 extracts from dietary spices and medicinal herbs were investigated by agar-well diffusion method against five foodborne bacteria (bacillus cereus, listeria monocytogenes, staphylococcus aureus, escherichia coli, and salmonella anatum). their total phenolic contents were also evaluated. many herb and spice extracts contained high levels of phenolics and exhibited antibacterial activity against foodborne pathogens. gram-positive bacteria were ... | 2007 | 17449125 |
| swarming behavior of and hemolysin bl secretion by bacillus cereus. | an association between swarming and hemolysin bl secretion was observed in a collection of 42 bacillus cereus isolates (p=0.029). the highest levels of toxin were detected in swarmers along with swarm cell differentiation (p=0.021), suggesting that swarming b. cereus strains may have a higher virulence potential than nonswarming strains. | 2007 | 17449693 |
| use of sodium hypochlorite (naocl) in laundering stops cross-contamination of bacillus cereus. | 2007 | 17452052 | |
| application of real-time quantitative pcr for the detection of selected bacterial pathogens during municipal wastewater treatment. | bacteria were detected at five stages of municipal wastewater treatment using taqman(r) real-time quantitative pcr (qpcr). thirteen probe and primer sets were tested for diverse pathogens that may be present in wastewater, including aeromonas hydrophila, bacillus cereus, clostridium perfringens, enterococcus faecalis, escherichia coli, e. coli o157:h7, helicobacter pylori, klebsiella pneumoniae, legionella pneumophila, listeria monocytogenes, pseudomonas aeruginosa, salmonella sp., and staphyloc ... | 2007 | 17462712 |
| confirmative electric dna array-based test for food poisoning bacillus cereus. | detection of the full set of toxin encoding genes involved in gastrointestinal diseases caused by b. cereus was performed. eight genes determining the b. cereus pathogenicity, which results in diarrhea or emesis, were simultaneously evaluated on a 16-position electrical chip microarray. the dna analyte preparation procedure comprising first 5 min of ultrasonic treatment, dna extraction, and afterwards an additional 10 min sonication, was established as the most effective way of sample processing ... | 2007 | 17466398 |
| phenotypic and genotypic diversity of bacillus cereus isolates recovered from honey. | of 433 honey samples collected in argentina, 114 (27%) yielded bacillus cereus isolates (27%) and 60 (14%) yielded other species of bacillus. the argentinian b. cereus isolates were compared with isolates recovered from honey from other countries (n=16) and with strain atcc 11718. the 133 b. cereus isolates and the atcc strain were characterized using rep-pcr fingerprinting with primers box, rep and eric, restriction fragment length polymorphism analysis of a 16s rrna gene fragment (16s rrna pcr ... | 2007 | 17466403 |
| competition and reproduction in mixed infections of pathogenic and non-pathogenic bacillus spp. | diamondback moth, plutella xylostella, larvae were infected with a primary pathogen, bacillus thuringiensis kurstaki (btk) in single strain and mixed infections. mixed infections comprised btk and a non-pathogenic isolate, either bacillus thuringiensis tenebrionis (btt) or bacillus cereus (bc). all strains reproduced in larval cadavers, but there was evidence of competition between different isolates within hosts. non-pathogenic isolates (btt, bc) had growth rates that were faster than btk in vi ... | 2007 | 17467004 |
| cytotoxicity in bacillus mojavensis is abolished following loss of surfactin synthesis: implications for assessment of toxicity and food poisoning potential. | bacillus subtilis and the closely related species bacillus pumilus and bacillus licheniformis have periodically been suggested to play a role in the aetiology of food poisoning despite the fact that the organisms do not possess the genes associated with enteropathogenicity in bacillus cereus. we show here that bacillus mojavensis, an organism closely related to b. subtilis, is able to produce toxic components which identify as a complex of three different surfactin analogues. these cyclic lipope ... | 2007 | 17467096 |
| biomedical evaluation of black lemurs (eulemur macaco macaco) in lokobe reserve, madagascar. | complete medical evaluations were performed on 25 wild black lemurs (eulemur macaco macaco) in lokobe reserve, northwestern madagascar. each animal received a complete physical examination. weight, body temperature, heart rate, and respiratory rate were recorded. blood samples were collected for complete blood cell count, differential white blood cell count, hemoparasite examination, serum biochemical profile, fat-soluble vitamin analysis, trace mineral analysis, and toxoplasma gondii and viral ... | 2007 | 17469278 |
| prevalence and genetic diversity of bacillus cereus in dried red pepper in korea. | bacillus cereus is a foodborne spore-forming bacterial pathogen that is ubiquitous in the natural environment. infections with this pathogen manifest as diarrheal or emetic types of food poisoning. in this study, 140 samples of dried red pepper purchased in korea were assayed for the presence of b. cereus according to the u.s. food and drug administration standard culture method. a multiplex pcr assay was developed for the rapid confirmation of b. cereus as an alternative to conventional biochem ... | 2007 | 17477261 |
| [endophthalmitis after plana vitrectomy: incidence, microbiology, and visual outcome]. | the aim of this retrospective study was to analyze incidence, microorganisms, and final visual acuity after pars plana vitrectomy. | 2007 | 17486032 |
| growth-related variations in the bacillus cereus secretome. | using 2-de, transcriptional gene fusions and cell cytotoxicity assays, we followed changes in the bacillus cereus strain atcc14579 secretome, gene expression and culture supernatant cytotoxicity from the end of the vegetative phase up to 5 h after entry into the stationary phase. the concentration of each of the 22 proteins in the culture supernatant was determined at various times. in addition, the stability of the proteins was studied. fifteen of these proteins, including 14 members of the vir ... | 2007 | 17486558 |
| vapor-phase activities of cinnamon, thyme, and oregano essential oils and key constituents against foodborne microorganisms. | the aim of the study presented here was to gain knowledge about the vapor-phase antimicrobial activity of selected essential oils and their major putatively active constituents against a range of foodborne bacterial and fungal strains. in a first step, the vapor-phase antimicrobial activities of three commercially available essential oils (eos)-cinnamon (cinnamomum zeylanicum), thyme (thymus vulgaris), and oregano (origanum vulgare)-were evaluated against a wide range of microorganisms, includin ... | 2007 | 17488023 |
| [isolation, molecular characterization and resistance mechanism study on a cadmium hyperresistant bacillus cereus]. | a gram-positive, aerobic, motile, spore-forming bacterium was isolated from a pb-zn mine tailing at suburb of beijing city. the bacterial strain isolated could resist cadmium efficiently and it was able to grow well on media plates containing 1 000 mg/l cadmium. the dna g + c content were found to be 60%. phylogenetic analysis of the 16s rrna gene revealed that this isolate was a member of the genus bacillus. two growth curves about this strain were drawn on media with and without cadmium. this ... | 2007 | 17489211 |