Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [viral origin of rheumatoid arthritis]. | 1987 | 2827142 | |
| molecular cloning of the cdna encoding the epstein-barr virus/c3d receptor (complement receptor type 2) of human b lymphocytes. | complementary dna clones for complement receptor type 2 (cr2), the b-lymphocyte membrane protein that serves as the receptor for epstein-barr virus and the c3d complement fragment, were obtained by screening a lambda gt11 library generated from raji b lymphoblastoid cell mrna. a 4.2-kilobase (kb) clone, representing the entire coding sequence of the protein plus untranslated 5' and 3' nucleotide sequences was obtained and sequenced. the 4.2-kb clone, which contains all but about 500 base pairs ( ... | 1987 | 2827171 |
| kawasaki disease and epstein-barr virus infection. | 1987 | 2827177 | |
| epstein-barr virus-specific cytotoxic t lymphocyte (ebv-ctl) activities in patients with kawasaki syndrome. | 1987 | 2827182 | |
| potential role of epstein-barr virus in sjögren's syndrome. | sjögren's syndrome is an autoimmune disease characterized by lymphocytic infiltration and destruction of salivary and lacrimal glands. this condition may occur as a primary condition or may be associated with other autoimmune disorders such as rheumatoid arthritis or systemic lupus. because the environmental factors that initiate ss are unknown, we have investigated the potential role of ebv, cmv, and other viruses. we observed that epithelial cells in salivary gland biopsies of patients with ss ... | 1987 | 2827246 |
| remarkable depression of cd4+2h4+ t cells in severe chronic active epstein-barr virus infection. | in order to better understand the features of chronic active epstein-barr (eb) virus infection, we employed two-colour immunofluorescence staining with monoclonal antibodies and flow cytometry analysis to study the lymphocyte phenotypes of two patients with severe symptoms of this disorder as well as four patients with mild symptoms. we found an increased number of activated t cells, as characterized by cd4+ia+, cd8+ia+, or cd4+tac+ phenotypes, and a markedly decreased cd4+2h4+ t cell subpopulat ... | 1987 | 2827299 |
| [analysis of the effects of phytohemagglutinin (pha) on inducers of epstein-barr virus antigens and dna synthesis]. | 1987 | 2827404 | |
| [the detection of epstein-barr virus dna in different kinds of nasopharyngeal biopsies]. | 1987 | 2827405 | |
| iga subclass distribution in peripheral blood lymphocyte cultures stimulated with lipopolysaccharide, pokeweed mitogen or epstein-barr virus. | 1987 | 2827442 | |
| herpesviridae in the coronary arteries and aorta of young trauma victims. | the presence of herpes simplex virus (hsv) and cytomegalovirus (cmv) nucleic acid and/or antigen was demonstrated in the coronary arteries and thoracic aortas of young trauma victims by the in situ dna hybridization and abc immunoperoxidase methods, respectively. epstein-barr virus (ebv) nucleic acid and capsid antigen were not detected in the arteries sampled in this study. of 8 subjects in which virus was detected in the coronary arteries, 6 were positive for hsv and 2 for cmv; of 7 cases posi ... | 1988 | 2827495 |
| comparison of the immune response to epstein-barr virus and cytomegalovirus in sera and synovial fluids of patients with rheumatoid arthritis. | the immune response against two herpesviruses has been determined in sera and matched synovial fluids of patients with rheumatoid arthritis (ra) and compared with that of a healthy control population. the increased level of antibody to epstein-barr virus (ebv) induced antigens in patients with ra resembles the antibody pattern observed against cytomegalovirus (cmv) induced antigens, which suggests the presence of a pathological condition in patients with ra that can reactivate latent viral infec ... | 1987 | 2827590 |
| expression of an early epstein-barr virus antigen (ea-d) in e. coli. brief report. | the 1.34 kb bcii-bgiii-fragment of the bamhi-m region of epstein-barr virus genome, comprising the complete bmrf1 open reading frame, was cloned into the tryptophan regulated e. coli expression vector path1. the resulting fusion protein, having a molecular weight of 80 kd, is recognized not only by anti-early antigen (ea)-positive human sera but also by the monoclonal antibody r3 directed against the diffuse component of ea (ea-d). a possible use for this fusion protein as an indicator protein i ... | 1987 | 2827613 |
| cytochemical, cytogenetic, immunophenotypic and tumorigenic characterization of two hairy cell lines. | two cell lines (eh and hk) were derived from two patients with hairy cell leukemia (hcl). both patients exhibited a clinical picture characteristic of hcl, including splenomegaly, cytopenias, and tartrate-resistant acid phosphatase (trap)-positive "hairy" lymphocytes in blood and marrow. eh and hk were demonstrably of b lineage, as judged by cytochemistry and immunophenotype, including expression of b1, b2, and leu-12 antigens and of monoclonal surface immunoglobulins (slgs). monoclonality was c ... | 1988 | 2827813 |
| diminished il-2-induced gamma-interferon production by unstimulated peripheral-blood lymphocytes in rheumatoid arthritis. | a diminished gamma-interferon (ifn-gamma) production by t cells from patients with rheumatoid arthritis (ra) in response to autologous stimulation coincides with a defective regulation of epstein-barr virus (ebv) transformation and is in part due to monocyte-produced interleukin-1 (il-1) inhibitor and prostaglandins. since il-2 can act directly on unstimulated t-cells to induce ifn-gamma production we have now examined the effect of recombinant il-2 (ril-2) on purified resting t lymphocytes from ... | 1988 | 2827831 |
| a major 50-kda human b-cell growth factor-ii induces both tac antigen expression and proliferation by several types of lymphocytes. | many cytokines have been documented to have a multiplicity of biological effects by acting on a variety of cells. in order to determine whether human bcgf-ii acts on any cells in addition to normal b cells, the effect of human bcgf-ii on murine thymocytes, human peripheral blood t cells, a human natural killer-like cell line, yt, and epstein-barr virus (ebv)-transformed b-cell lines was further examined. bcgf-ii augmented incorporation of [3h]thymidine by murine thymocytes in combination with su ... | 1988 | 2827895 |
| epstein-barr virus infections in children. | 1987 | 2827962 | |
| site-specific cleavage of a fusion protein by renin. | a double-stranded synthetic oligonucleotide that codes for an amino acid sequence specifically recognized and cleaved by the endopeptidase, renin, was inserted into a plasmid expression vector. the double-stranded oligonucleotide was placed at the junction between the sequences coding for two distinct domains of a fusion protein. the vector used for this analysis expressed a 190-kd epstein-barr virus membrane antigen (ebv-ma)-beta-galactosidase (beta-gal) fusion protein (beisel et al., 1985). th ... | 1987 | 2827977 |
| in vitro expansion of epstein-barr virus-specific hla-restricted cytotoxic t cells direct from the blood of infectious mononucleosis patients. | the cytotoxic t-cell response induced by primary epstein-barr virus (ebv) infection and detectable in the blood of infectious mononucleosis (im) patients shows several unusual features when tested in in vitro assays. lysis of ebv-transformed target lines occurs with no apparent hla restriction, and the putative ebv specificity of the response has been seriously questioned. in the present work we show that the primary t-cell response in im is polyclonal and indeed does contain a virus-specific hl ... | 1987 | 2828228 |
| interleukin-1-like activity produced by hybrids constructed with epstein-barr-virus-transformed human b lymphocytes and mouse myeloma cells. | peripheral blood b lymphocytes from a donor with positive tuberculin skin test reaction were transformed into lymphoblastoid cell lines by epstein-barr virus and then fused by polyethylene glycol with mouse myeloma cells. human-mouse hybrid cells producing human igm monoclonal antibody to purified protein derivative of tuberculin were isolated, and the concentrated supernatant of one of these cell hybrids was tested for the capacity of interfering with dna synthesis of human and mouse lymphocyte ... | 1988 | 2828240 |
| evaluation of latex-based heterophile antibody assay for diagnosis of acute infectious mononucleosis. | the diagnosis of an acute episode of infectious mononucleosis (im) is made on the basis of clinical symptoms and serological evidence for development of heterophile antibodies. in an effort to obtain a very sensitive and specific assay for diagnosis of im, we have developed meristar:im. the meristar assay uses latex beads coated with purified bovine heterophile antigen. we compared the sensitivity and specificity and performance characteristics of the latex-based test to those of the commerciall ... | 1987 | 2828417 |
| history, pharmacokinetics, and pharmacology of acyclovir. | a screening program for antiviral drugs begun at burroughs wellcome in the 1960s resulted in the discovery of acyclovir in 1974. preclinical investigation brought the drug to clinical trials in 1977 and the first form of the drug (topical) was available to physicians in 1982. activity of acyclovir is greatest against herpes 1 and herpes 2, less against varicella zoster, still less against epstein-barr, and very little against cytomegalovirus. acyclovir is an antiviral agent only after it is phos ... | 1988 | 2828440 |
| human monoclonal antibody directed against gag gene products of the human immunodeficiency virus. | tonsillar b lymphocytes from an asymptomatic individual infected with hiv were transformed with ebv. a cloned lymphoblastoid cell line was obtained that secreted human mab (igg4 subtype) against the known gag gene products of 55,000 and 25,000 da and a protein of 40,000 da. the latter p40 protein appears to be a hiv gag-related gene product that is also recognized immunologically by individuals infected by hiv. although the mab detects infection of cells by different hiv isolates, it does not ne ... | 1988 | 2828474 |
| the high efficiency, human b cell immortalizing heteromyeloma cb-f7. production of human monoclonal antibodies to human immunodeficiency virus. | this paper describes the construction of a new heteromyeloma cell line designated cb-f7. the cell line was derived from xenogeneic somatic cell hybridization between normal human b lymphocytes and the murine hat-sensitive p3x63ag8/653 cell line. cb-f7 cells were characterized by rapid cell growth (doubling time about 16 h) and high cloning efficiencies in culture medium supplemented with 10% or 5% fetal calf serum, respectively. the karyotype of the cells consists of about 75-78 chromosomes as w ... | 1988 | 2828478 |
| burkitt lymphoma in a child with human immunodeficiency virus infection. | 1988 | 2828591 | |
| survey of nonhuman primates for antibodies reactive with epstein-barr virus (ebv) antigens and susceptibility of their lymphocytes for immortalization with ebv. | the susceptibility to transformation with epstein-barr virus (ebv) and the prevalence of antibodies reactive to ebv were examined in 43 primate species. in vitro ebv infection was revealed in lymphocytes from old world monkeys, including patas monkeys and the colobines, as well as in lymphocytes from the apes. antibodies reactive to ebv-early antigen/viral capsid antigen (ea/vca) were detected in all the species of old world monkeys and apes examined and in two out of seven species of new world ... | 1987 | 2828628 |
| seroepidemiology of epstein-barr virus infections in a developing country. | 1987 | 2828648 | |
| conservation of gene organization in the lymphotropic herpesviruses herpesvirus saimiri and epstein-barr virus. | by analyses of short dna sequences, we have deduced the overall arrangement of genes in the (a + t)-rich coding sequences of herpesvirus saimiri (hvs) relative to the arrangements of homologous genes in the (g + c)-rich coding sequences of the epstein-barr virus (ebv) genome and the (a + t)-rich sequences of the varicella-zoster virus (vzv) genome. fragments of hvs dna from 13 separate sites within the 111 kilobase pairs of the light dna coding sequences of the genome were subcloned into m13 vec ... | 1988 | 2828671 |
| different patterns of epstein-barr virus gene expression and of cytotoxic t-cell recognition in b-cell lines infected with transforming (b95.8) or nontransforming (p3hr1) virus strains. | epstein-barr virus (ebv)-negative burkitt's lymphoma (bl) cell lines have been converted to ebv genome positivity by in vitro infection with the transforming ebv strain b95.8 and with the nontransforming mutant strain p3hr1, which has a deletion in the gene encoding the nuclear antigen ebna2. these b95.8- and p3hr1-converted lines have been compared for their patterns of expression of ebv latent genes (i.e., those viral genes constitutively expressed in all ebv-transformed lines of normal b-cell ... | 1988 | 2828684 |
| structural analyses of eber1 and eber2 ribonucleoprotein particles present in epstein-barr virus-infected cells. | the ribonucleoprotein (rnp) particles containing the epstein-barr virus-associated small rnas eber1 and eber2 were analyzed to determine their rna secondary structures and sites of rna-protein interaction. the secondary structures were probed with nucleases and by chemical modification with single-strand-specific reagents, and the sites of modification or cleavage were mapped by primer extension. these data were used to develop secondary structures for the two rnas, and likely sites of close rna ... | 1988 | 2828685 |
| b-cell lymphoproliferation and lymphomagenesis are associated with clonotypic intracellular terminal regions of the epstein-barr virus. | we analyzed 17 b-cell lineages cloned from two patients with infectious mononucleosis and found that different b-cell lineages exhibited notable variation in the length of the fused epstein-barr virus (ebv) terminal region on intracellular ebv episomes. ebv termini in different b-cell clones from the same person differed by as many as 15 to 20 reiterations of the ca. 500-base-pair terminal repeat sequence. in contrast, analysis of seven b-cell lineages cloned from a patient with a fatal, oligocl ... | 1988 | 2828691 |
| viral etiology of testicular tumors. | testicular carcinoma and hodgkin's disease are among the most frequent malignancies afflicting young men in the 15 to 39-year age group. these malignancies share other epidemiological characteristics as well, including multiple histological tumor types, higher rates of occurrence in white, urbanized populations and upper social classes, relative infrequency among black populations, low but definite familial occurrence and an early geographically acquired lifetime risk irrespective of later migra ... | 1988 | 2828695 |
| prior herpes simplex virus type 2 infection as a risk factor for hiv infection. | antibodies to cytomegalovirus (cmv), epstein-barr virus (ebv), and herpes simplex viruses types 1 and 2 (hsv-1, hsv-2) in three sequential serum samples from 62 men who did and 61 men who did not develop human immunodeficiency virus (hiv) infection at the time of the final (third) serum specimen were studied. antibody titers to cmv, ebv, and hsv-1 did not significantly rise in or differ between men who did or did not get hiv infection. however, we found that 32 (68%) of 47 hiv seroconverters had ... | 1988 | 2828700 |
| specificity of routine parasite serological tests in autoimmune disorders, neoplastic disease, ebv-induced mononucleosis, and hiv infection. | sera from 120 patients with rheumatological disorders, neoplastic disease, infectious mononucleosis, and hiv infection, and from 30 healthy blood donors were tested for nonspecific reactivity in 13 routinely used parasite serological tests. responses were detected in 3/30 healthy blood donors (10%) vs 25/120 patients (21%). of 40 responses in these 28 responders most were weakly reactive, and 25 out of 40 responses were only at borderline level. response rates were highest in patients with monon ... | 1987 | 2828756 |
| virus receptors on lymphoid cells. | the studies described above indicate the advances made in the isolation and characterization of virus receptors of lymphoreticular cells (table i). although the examples of lymphotropic virus receptors cited in this chapter indicate that single membrane glycoproteins can serve as receptors, other nonlymphoid viruses such as vesicular stomatitis virus (vsv) (table i) appear to utilize glycolipid or phospholipid components for cell attachment. these molecules may be responsible for the broad speci ... | 1987 | 2828828 |
| receptors for insulin and growth hormone on lymphoid cells. | 1987 | 2828829 | |
| human monoclonal antibodies to pseudomonas aeruginosa produced by ebv-transformed cells. | numerous lymphoblastoid cell lines (lcls) which secreted antibodies against pseudomonas aeruginosa (all fisher's immunotypes and homma's immunotype 1) were established by epstein-barr virus (ebv)-transformation of lymphocytes. five lcls were established as long-term culture lines and their properties were determined. these lcls produced monoclonal antibodies to fisher's immunotype 1 and 4 and homma's immunotype 1, and their immunoglobulin classes were igm, igg, and iga. we found that three monoc ... | 1987 | 2828894 |
| ['it must be a virus...']. | 1988 | 2829039 | |
| corneal subepithelial infiltrates associated with systemic epstein-barr viral infection. | corneal subepithelial infiltration resembling adenoviral keratitis developed in two patients with serologic evidence of systemic epstein-barr viral (ebv) infection and nondetectable antibody levels for adenovirus (adv). these cases suggest that ebv is capable of mimicking more commonly recognized corneal infectious disease entities. | 1987 | 2829091 |
| the many faces of epstein-barr virus. | epstein-barr virus (ebv) persists for life in a person who has been infected. investigators are uncertain what this means to the host, particularly an immunocompromised one. ebv is the most common cause of infectious mononucleosis, but the diagnosis must be based on clinical, hematologic, and serologic criteria because other agents are the cause in about 10% of cases. ebv is the first virus to be associated with a neoplasm--burkitt's lymphoma. this childhood malignancy is relatively common in ce ... | 1988 | 2829148 |
| establishment and characterization of human leukemia cell lines (j6-1, j6-2 and j6-3). | 1986 | 2829161 | |
| precursor b cells transformed by epstein-barr virus undergo sterile plasma-cell differentiation: j-chain expression without immunoglobulin. | human bone marrow cells were depleted of b lymphocytes to enrich for precursor b cells that could be transformed with epstein-barr virus. transformed immunoglobulin-negative precursors either maintained their immunoglobulin genes in the germ-line configuration or had undergone dj or abortive vdj rearrangements (v, d, and j represent variable, diversity, and joining gene segments). all cell lines and their derivative clones, even those with no detectable immunoglobulin gene rearrangements, genera ... | 1988 | 2829207 |
| bamhi e region of the epstein-barr virus genome encodes three transformation-associated nuclear proteins. | recombinant vectors carrying dna fragments from the bamhi e region of the b95-8 epstein-barr virus (ebv) genome were transfected into cos-1 cells, and the transient expression of ebv-encoded nuclear antigens (ebnas) was analyzed by using polyvalent human antisera and rabbit antibodies to synthetic peptides. vector dna containing two rightward open reading frames in the bamhi e fragment, berf2a and berf2b, induced the expression of a nuclear antigen identical serologically and with respect to siz ... | 1988 | 2829223 |
| [cytotoxicity induced by the serum of patients with aids and at high risk of aids against cells infected with epstein-barr virus]. | serologic reactivity against ebv-infected lines was assayed in patients with aids (n = 4) or the aids related complex (aids-rc) (n = 46) residing in argentina. anti-vca serology was comparable to that of controls. however sera from aids and aids-rc could induce antibody dependent cell mediated cytotoxicity (adcc) to ebv-infected cell lines (p3hrik and raji). adcc activity could be recovered in the high molecular weight fractions of aids and aids-rc sera. adcc was observed in sera with high level ... | 1985 | 2829276 |
| [pulmonary complications following bone marrow transplantation as seen on the x-ray]. | in a retrospective study chest radiographs of 87 bone marrow transplant recipients were analysed. 36 patients had pulmonary complications with lung opacifications. interstitial changes were more frequent than airspace pneumonias. the latter were caused by bacteria and fungi only. the most common cause of pulmonary complications was cytomegalovirus pneumonia. it was characterised uniformly by a bilateral diffuse interstitial pattern. idiopathic interstitial pneumonias were indistinguishable from ... | 1988 | 2829300 |
| monocyte-derived human b-cell growth factor identified as interferon-beta 2 (bsf-2, il-6). | soluble products of either epstein-barr virus (ebv)-infected b cells or activated monocytes promote the proliferation of ebv-infected b cells and permit their growth at low cell densities. this suggests that growth factors are important for b-cell immortalization by ebv. in this study, a monocyte-derived factor that promotes the growth of ebv-infected b cells was purified and identified as interferon-beta 2 (ifn-beta 2), which is also known as 26-kilodalton protein, b-cell differentiation factor ... | 1988 | 2829354 |
| molecular analysis of hla-dp specificities hla-dpw1, -dpw2 and -dpw4: dp beta chain heterogeneity correlates with plt subtyping. | hla-dp molecules were isolated from epstein-barr virus transformed b cell lines by immunoprecipitation with monoclonal antibody b7/21.2 and subsequently analysed by two-dimensional gel electrophoresis. the results obtained demonstrate that the hla-dp molecules that can be isolated from cells positive for the hla-dp specificities hla-dpw1, -dpw2 and -dpw4 display dp beta chain isoelectric point differences, whereas no dp alpha chain polymorphism was observed. these results suggest that the plt de ... | 1988 | 2829386 |
| increase of protein synthesis by uridine supplement in lectin-stimulated peripheral blood lymphocytes and eb virus-transformed b cell line of hereditary orotic aciduria type i. | a 2 month-old japanese girl with hereditary orotic aciduria type i was treated with oral uridine supplement. the activities of orotate phosphoribosyltransferase (oprt) and orotidine-5'-phosphate decarboxylase (odc) in erythrocytes were 2.7 and 0.4%, respectively, of those in the controls. megaloblastic anemia, excessive urinary excretion of orotic acid, lymphopenia and decreased number of okt3 positive lymphocytes on admission were corrected after the uridine supplement. peripheral blood lymphoc ... | 1987 | 2829387 |
| tpa-inducible epstein-barr virus genes in raji cells and their regulation. | a number of agents including the tumor promoter 12-o-tetradecanoylphorbol-13-acetate (tpa) can induce an abortive virus cycle in the ebv nonproducer burkitt's lymphoma line raji. we describe the pattern of viral rnas transcribed in uninduced cells and in cells treated with tpa for 8 hr, as analyzed by northern blotting. by comparing the patterns of rnas observed in cells treated with tpa, tpa plus cycloheximide, or cycloheximide alone, we have tested whether any ebv gene in tpa-treated raji cell ... | 1988 | 2829433 |
| neutrophil lactoferrin content in viral infections. | in an attempt to elucidate the previously observed decrease in plasma lactoferrin-neutrophil ratio in subjects with acute viral infections, a study of the neutrophil lactoferrin content in such infections was undertaken. with the use of an immunoperoxidase stain for lactoferrin, neutrophils in viral illness were shown to have reduced lactoferrin content (mean score 97.9 +/- sd [38.0] per 100 neutrophils) as compared with normal subjects (mean score 196.4 +/- sd [3.6] per 100 neutrophils) (t = 7. ... | 1988 | 2829617 |
| ebv transformation and microfusion as the potential source of human monoclonal antisperm antibodies. | peripheral blood lymphocytes were obtained from vasectomized men with high serum titers of antisperm antibodies. an epstein-barr virus (ebv) transformation was performed either with b cells or mononuclear leukocytes. the effect of feeder cells (irradiated umbilical cord blood lymphocytes), cyclosporin a, and in vitro stimulation of lymphocytes with sperm extract on ebv transformation was evaluated. antibody-producing cells were screened for specificity against human sperm by an enzyme-linked imm ... | 1987 | 2829635 |
| intravenous immunoglobulin: new clinical applications. | at the present time there are three commercially available intravenous immunoglobulin (ivig) preparations. there is no distinct therapeutic advantage for any one product over any other. intravenous immunoglobulin is currently approved for the treatment of antibody deficiency syndromes and for acute and chronic idiopathic thrombocytopenic purpura. in addition, controlled clinical trials have demonstrated efficacy for the treatment of kawasaki disease and for the prevention of the following infect ... | 1988 | 2829663 |
| treatment of severe epstein-barr virus-induced polyclonal b-lymphocyte proliferation by anti-b-cell monoclonal antibodies. two cases after hla-mismatched bone marrow transplantation. | we treated two children who developed epstein-barr virus-induced polyclonal b-cell proliferation after hla-mismatched bone marrow transplantation for congenital immunodeficiency with two monoclonal anti-b-cell antibodies. lymphoproliferative syndrome occurred between 50 and 60 days after bone marrow infusion, and was diagnosed by the presence of spontaneously growing b cells containing epstein-barr-nuclear antigen in the blood and bone marrow. the mouse monoclonal anti-b-cell antibodies used wer ... | 1988 | 2829673 |
| chronic fatigue syndrome: a working case definition. | the chronic epstein-barr virus syndrome is a poorly defined symptom complex characterized primarily by chronic or recurrent debilitating fatigue and various combinations of other symptoms, including sore throat, lymph node pain and tenderness, headache, myalgia, and arthralgias. although the syndrome has received recent attention, and has been diagnosed in many patients, the chronic epstein-barr virus syndrome has not been defined consistently. despite the name of the syndrome, both the diagnost ... | 1988 | 2829679 |
| granuloma annulare-like eruption due to chronic epstein-barr virus infection. | a 32-year-old woman afflicted with a severe, chronic epstein-barr virus (cebv) infection of 12 months' duration developed an unusual, granuloma annulare-like eruption. the cutaneous disorder, which paralleled the clinical course of her disease, was characterized by slightly raised, erythematous, annular lesions predominantly involving the face and arms, with sporadic involvement elsewhere. histopathologic examination of biopsy material obtained from affected skin disclosed a granulomatous dermat ... | 1988 | 2829748 |
| [detection of epstein-barr virus nucleic acid sequences in non-hodgkin's lymphoma]. | tumor tissue specimens of 25 patients with various entities of non-hodgkin's lymphomas were examined for the presence of epstein-barr virus (ebv) nucleic acids and ebv nuclear antigens (ebna) by in situ hybridization and anticomplement immunofluorescence. in tumor cells of five patients ebv nucleic acid and ebna were demonstrated. the histopathologic examinations revealed in these cases one burkitt's lymphoma, two centroblastic lymphomas and each one lymphoplasmocytic and lymphocytic lymphoma. | 1987 | 2829788 |
| carboxyethyl gamma-aminobutyric acid, a polyamine derivative, improves the recovery of ebv-transformed lymphocytes. | a polyamine derivative, carboxyethyl - aminobutyric acid (cegaba), induces the formation of a large number of epstein-barr virus (ebv) transformed lymphocytes, when added to the culture medium immediately after ebv infection. however, cegaba shows only a moderate effect on the stability of ebv-transformed cell lines over time, and does not affect the growth of stabilized cell lines. it is possible that cegaba acts on cells other than ebv transformed lymphocytes (in fact, after ebv infection all ... | 1988 | 2829900 |
| effects of human immunodeficiency virus (hiv) on the cytotoxic response to epstein barr virus (ebv) transformed b lymphocytes. | the human immunodeficiency virus (hiv) may interact with the epstein barr virus (ebv) indirectly by effects on the t4 lymphocyte or directly by effects on ebv transformed b lymphocytes. we have confirmed the susceptibility of ebv transformed b lymphocytes to productive hiv infection, and have evaluated the cytotoxic activity of hiv seronegative and seropositive donors after sensitization by their autologous ebv infected (monoinfected) or ebv and hiv infected (coinfected) transformed cell lines i ... | 1987 | 2829951 |
| an ebv-based mammalian cell expression vector for efficient expression of cloned coding sequences. | the construction of a mammalian cell expression vector using human cytomegalovirus immediate early gene enhancer to initiate transcription of inserted coding sequences is described. the vector also carries epstein-barr virus ebna-1 nuclear antigen gene, ori-p sequences and hygromycin b resistance gene hph from e. coli. the expression capacity of this construct was tested by inserting the chloramphenicol acetyltransferase (cat) gene into the vector. the ebv-cat construct was transfected into vari ... | 1988 | 2829966 |
| a human-human hybridoma antibody (trb12) defining subgroups of hla-dqw1 and -dqw3. | we have constructed an igg, kappa human--human hybridoma ab(trb12), which precipitates a molecule consisting of two polypeptides of about 33 and 27 kd in size. trb12 reacted with; (1) 7 out of 10 dqw1-positive cell lines in iif, and all 10 in a rosette-assay; (2) 5 out of 12 dqw3-positive cells, both in iif and the rosette-assay; (3) none of 4 dqw2 homozygous cells. detergent cell lysate of dqw1 homozygous cell lines contained antigens that cross-linked the mouse monoclonal antibody genox 353 g2 ... | 1987 | 2830209 |
| comparative evaluation of three commercial tests for detection of heterophile antibody in patients with infectious mononucleosis. | infectious mononucleosis (im) is caused by the epstein-barr virus. commonly used laboratory tests used for diagnosis of im include a screening test based on the observation that horse erythrocytes are agglutinated by the paul-bunnell antibody found in the serum of patients with im. this study evaluated two latex agglutination (la) kits for im, monolatex (wampole laboratories) and immunoscan-im (american microscan) (formerly monogen; biokit, s.a.), and compared them with monospot (ortho diagnosti ... | 1988 | 2830311 |
| b lymphoblastoid cell lines with rearranged t cell receptor beta-chain genes retain conventional b cell surface antigen and ig expression. | transformation of peripheral blood lymphocytes by co-incubation with ebv produces b lymphoblastoid cell lines, but rearrangement of tcr beta-chain genes was observed in three different cell lines derived from two individuals. because rearrangement of tcr genes in b lymphocytes is considered a rare event, these b lymphoblastoid cell lines with rearranged tcr beta-genes were examined in detail to determine whether there were any additional characteristics to distinguish them from b lymphoblastoid ... | 1988 | 2830338 |
| the chronic mononucleosis syndrome. | 1988 | 2830340 | |
| acute cerebellar syndrome complicating infectious mononucleosis. | 1987 | 2830369 | |
| epstein-barr virus and epithelial cells: implications for the otolaryngologist. | epstein-barr virus (ebv) is an oncogenic herpesvirus associated with two human malignancies. initially linked in the etiology of burkitt's lymphoma, a b cell lymphoma, it has subsequently been implicated in the causation of nasopharyngeal carcinoma, an epithelial tumor of considerable world health importance. more recently a number of head and neck tumors have been shown to harbor the virus, suggesting a possible role for ebv in their development. | 1988 | 2830415 |
| cytotoxic t-cell clones discriminate between a- and b-type epstein-barr virus transformants. | epstein-barr virus (ebv) is the aetiological agent of infectious mononucleosis and is associated with burkitt's lymphoma and nasopharyngeal carcinoma. the virus is harboured for life in all previously infected individuals and is apparently controlled by a population of ebv-specific memory t lymphocytes, specifically activated to recognize the functionally defined lymphocyte-detected membrane antigen. two types (a and b) of ebv have been identified that show dna sequence divergence within the bam ... | 1988 | 2830541 |
| chronic fatigue syndrome--a diagnosis for consideration. | chronic fatigue syndrome (cfs) is an illness which may be mild or completely disabling. clients who return with recurring non-related symptoms and no specific diagnosis may suffer from cfs. the symptoms of cfs are numerous and varied, including fatigue, malaise, myalgias, difficulty concentrating, headaches and sore throat. patient complaints seem out of proportion to the physical findings, which may be normal. there is no cure for this chronic disease. therapy is primarily symptomatic. the role ... | 1988 | 2830563 |
| an epstein-barr virus immediate-early gene product trans-activates gene expression from the human immunodeficiency virus long terminal repeat. | acquired immunodeficiency syndrome patients are frequently coinfected with epstein-barr virus (ebv). in this report, we demonstrate that an ebv immediate-early gene product, bamhi mlf1, stimulates expression of the bacterial chloramphenicol acetyltransferase (cat) gene linked to the human immunodeficiency virus (hiv) promoter. the hiv promoter sequences necessary for trans-activation by ebv do not include the tat-responsive sequences. in addition, in contrast to the other herpesvirus trans-activ ... | 1988 | 2830625 |
| epstein-barr virus transformation. | 1987 | 2830637 | |
| [reactivity of 48-1 antibody with human liver tissue]. | reactivity of monoclonal antibody (48-1), produced by the epstein-barr virus (ebv) transformation method using lymphocytes from a chimpanzee infected with non-a, non-b hepatitis, on human liver biopsy specimens from 240 cases was studied. by means of indirect immunoperoxidase study (secondary antibody: horseradish peroxidase labeled anti-human igm.f(ab')2), the cases with non-a, non-b acute hepatitis showed a high positive reaction (15/24), while those cases with a-type and b-type hepatitis show ... | 1987 | 2830652 |
| reactivation of epstein-barr virus in sjögren's syndrome. | sjögren's syndrome (ss) is an autoimmune disease characterized by lymphocytic infiltration and destruction of salivary and lacrimal glands. this condition may be limited to glandular tissues or may be associated with other autoimmune disorders such as rheumatoid arthritis or systemic lupus erythematosus. since the environmental factors that initiate ss are unknown, we have investigated the potential role of epstein-barr virus (ebv), cytomegalovirus (cmv) and other viruses. we observed that epith ... | 1987 | 2830658 |
| a gene for dihydrofolate reductase in a herpesvirus. | the enzyme dihydrofolate reductase (dhfr) is found ubiquitously in both prokaryotes and eukaryotes. it is essential for de novo synthesis of purines and of deoxythymidine monophosphate for dna synthesis. among viruses, however, only the t-even and t5 bacteriophage have been found to encode their own dhfr. in this study a gene for dhfr was found in a specific subgroup of the gamma or lymphotropic class of herpesviruses. dna sequences for dhfr were found in herpesvirus saimiri and herpesvirus atel ... | 1988 | 2830673 |
| mechanism of activation and biological role of the c-myc oncogene in b-cell lymphomagenesis. | 1987 | 2830825 | |
| studies on cell-mediated immune defects to epstein-barr virus and cytomegalovirus in hiv-related disorders. | 1987 | 2830827 | |
| serological follow-up study on the antibody levels to epstein-barr virus-determined nuclear antigen (ebna) patients with nasopharyngeal carcinoma (npc) after radiation therapy. | serological follow-up studies for up to 4 years on the levels of igg antibody to ebv-determined nuclear antigen (ebna) were carried out on 36 patients with nasopharyngeal carcinoma (npc). the serum levels of iga antibody specific to ebv capsid antigen (vca) were also measured in some of the patients. the titers of ebna antibody were measured by enzyme-linked immunosorbent assay (elisa) and those of iga antibody to vca were measured by the indirect immunofluorescence method. the ebna antibody tit ... | 1987 | 2830879 |
| mycobacterium leprae-reactive t-cell clones isolated from polar lepromatous and tuberculoid leprosy patients. | t-cell clones capable of mounting a proliferative response to mycobacterium leprae were obtained in three leprosy patients (two polar lepromatous and one polar tuberculoid) either from m. leprae-activated or from protein-purified derivative-activated polyclonal t lymphoblasts. all these clones expressed the cd4 surface marker. some of them proliferated to the antigen only in the presence of interleukin-2. a majority expressed cross-reactive responses to other mycobacteria. clones obtained from t ... | 1987 | 2830893 |
| polyspecific natural antibodies and autoantibodies secreted by human lymphocytes immortalized with epstein-barr virus. | recent studies have shown that autoreactive b cells and autoantibodies are present in pathological as well as in normal situations. in the present study, we immortalized human b cell lines from normal individuals and from patients with malignant or benign dysglobulinemia with epstein-barr virus and examined, after cloning, the autoantibody reactivities of the immunoglobulins secreted by these cells. forty-two supernatants were analyzed by enzyme-immunoassay on a panel of 13 self and non-self ant ... | 1988 | 2830925 |
| sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of ebv in a lymphoma line. | here we describe development and application of highly sensitive fluorescence methodology for localization of single-copy sequences in interphase nuclei and metaphase chromosomes by nonisotopic in situ hybridization. application of this methodology to the investigation of epstein-barr virus integration in the namalwa lymphoma cell line has revealed two ebv genomes closely integrated at the known site on chromosome 1. detecting sequences as small as 5 kb, we further demonstrate resolution within ... | 1988 | 2830981 |
| human recombinant interleukin 4 induces normal b cells to produce soluble cd23/ige-binding factor analogous to that spontaneously released by lymphoblastoid b cell lines. | surface-labeled epstein-barr virus (ebv)-transformed lymphoblastoid rpmi 8866 cells release in their supernatant a radiolabeled 25-kda polypeptide which reacts with the fc epsilon rl/cd23-specific monoclonal antibody (mab) 25 and which binds to ige but not igg (ige bf/scd23). ige bf/scd23 had an isoelectric point of 4.5-5.0. the reactivity of mab 25 with ige bf/scd23 allowed us to set up a radioimmunoassay for detection of ige bf/scd23 in cell culture supernatants. supernatants from fc epsilon r ... | 1988 | 2831064 |
| qualitative and quantitative differences between burkitt lymphoma and lymphoblastoid cell lines in the expression of membrane ecto-nucleotidases and in the response to agonists of the a2-type adenosine receptor. | the activity of ecto-nucleotidases has been determined on intact cells from burkitt lymphoma (bl) and lymphoblastoid cell lines established in vitro (lcls). bl cells never express ecto-5'-nucleotidase (5'-n) and exhibit only low levels of ecto-atpase activity, whereas lcl-cells usually express both enzymes to varying degrees. there is a certain correlation (r = 0.75) between 5'-n and ecto-atpase. when camp formation in response to agonists of the a2-type (stimulating) adenosine receptor is measu ... | 1988 | 2831160 |
| binding and tyrosine kinase activities of the insulin receptor on epstein-barr virus transformed lymphocytes from patients with werner's syndrome. | to assess the abnormalities of insulin receptor in patients with werner's syndrome, we established epstein-barr virus transformed lymphocytes and studied the binding as well as kinase activities of insulin receptor. the insulin binding to both intact cells and wga-purified insulin receptor preparations was within normal range. autophosphorylation of the beta-subunit was not altered in patients with werner's syndrome, and the receptors of these patients phosphorylated an exogenous substrate to a ... | 1988 | 2831266 |
| subclass of individual iga-secreting human lymphocytes. investigation of in vivo pneumococcal polysaccharide-induced and in vitro mitogen-induced blood b cells by monolayer plaque-forming cell assays. | the subclass of individual human iga b cells was investigated by means of monolayer plaque-forming cell assays permitting analysis of all iga-secreting cells as well as of cells secreting iga anti-pneumococcal polysaccharide antibody. center cells were examined by indirect immunofluorescence staining with mouse mab against either of the two iga subclasses as primary antibodies and fitc-conjugated rabbit anti-mouse ig as the second antibody. blood lymphocytes spontaneously secreting iga (mean 399 ... | 1988 | 2831268 |
| cr2 is a complement activator and the covalent binding site for c3 during alternative pathway activation by raji cells. | antibody-independent activation of the alternative c pathway by human lymphoblastoid cell lines latently infected with ebv has been recognized for some time, although the mechanisms involved and the specific cell surface molecule(s) recognized by the c system have not been identified. the present studies, carried out with the purified proteins of the alternative pathway have addressed these questions. activation of the purified proteins of the alternative pathway by raji lymphoblastoid cells was ... | 1988 | 2831273 |
| activation of hla-restricted ebv-specific cytotoxic t cells does not require cd4+ (helper) t cells or exogenous cytokines. | mhc-restricted, viral ag-specific "memory" ctl are thought to play a decisive role in the defense against pathogenic viruses. however, the requirements for activating such ctl remain controversial. in particular, the role of cd4+ helper cells and their soluble products (e.g., il-2) are uncertain. to approach these questions as they relate to ebv-specific ctl, highly purified cd8+ t cells from healthy ebv-seropositive individuals were cultured with autologous irradiated ebv-transformed b lymphobl ... | 1988 | 2831276 |
| evaluation and management of nasopharyngeal carcinoma. | 1988 | 2831295 | |
| [comparison of epstein-barr virus (ebv)-associated polypeptides from different strains of ebv]. | 1987 | 2831322 | |
| [transfection of human nasopharyngeal epithelial cells with epstein-barr virus dna derived from nasopharyngeal carcinoma]. | 1987 | 2831323 | |
| identification of the epstein-barr virus gp85 gene. | the epstein-barr virus glycoprotein gp85 has been mapped to the epstein-barr virus dna open reading frame bxlf2 (r. baer, a. bankier, m. biggin, p. deininger, p. farrell, t. gibson, g. hatfull, g. hudson, s. stachwell, c. sequin, p. tufnell, and b. barrell, nature [london] 310:207-211, 1984). a gp85-specific monoclonal antibody reacts with the bxlf2 in vitro transcription-translation product. the monoclonal antibody also precipitates an 85-kilodalton protein from rodent cells transfected with th ... | 1988 | 2831372 |
| induction of antibodies to the epstein-barr virus glycoprotein gp85 with a synthetic peptide corresponding to a sequence in the bxlf2 open reading frame. | epstein-barr virus codes for at least three envelope glycoproteins, one of which, gp85, has not yet been mapped to the viral genome. the publication and analysis of the entire epstein-barr virus dna sequence has allowed identification of open reading frames with potential for encoding membrane glycoproteins. to determine whether one of these candidate open reading frames, bxlf2, codes for gp85, an antibody was made to a 17-residue peptide derived from positions 518 to 533 of the predicted bxlf2 ... | 1988 | 2831373 |
| a fifth epstein-barr virus nuclear protein (ebna3c) is expressed in latently infected growth-transformed lymphocytes. | three distantly homologous neighboring long open reading frames in the epstein-barr virus (ebv) genome are preceded by short open reading frames. the leftmost short and long open reading frames encode ebna3, a nuclear protein which is slightly smaller (145 kilodaltons [kda]) than two other nuclear proteins (150 to 155 kda) detected in western blots (immunoblots) of latently infected cell protein (k. hennessy, f. wang, e. woodland-bushman, and e. kieff, proc. natl. acad. sci. usa 83:5693-5697, 19 ... | 1988 | 2831394 |
| location, transcript analysis, and partial nucleotide sequence of the cytomegalovirus gene encoding an early dna-binding protein with similarities to icp8 of herpes simplex virus type 1. | the results presented here locate the gene encoding an early, nonvirion, single-stranded dna-binding protein of human and simian strains of cytomegalovirus (cmv) [hcmv(towne) db140 and scmv(colburn) db129, respectively] and provide additional evidence that this protein is the cmv homolog of the herpes simplex virus type 1 (hsv-1) major dna-binding protein (icp8), as proposed earlier (d. g. anders, a. irmiere, and w. gibson, j. virol. 58:253-262). the icp8 gene was used as a probe in southern ana ... | 1988 | 2831398 |
| identification and expression of a human cytomegalovirus glycoprotein with homology to the epstein-barr virus bxlf2 product, varicella-zoster virus gpiii, and herpes simplex virus type 1 glycoprotein h. | an open reading frame with the characteristics of a glycoprotein-coding sequence was identified by nucleotide sequencing of human cytomegalovirus (hcmv) genomic dna. the predicted amino acid sequence was homologous with glycoprotein h of herpes simplex virus type 1 and the homologous protein of epstein-barr virus (bxlf2 gene product) and varicella-zoster virus (gpiii). recombinant vaccinia viruses that expressed this gene were constructed. a glycoprotein of approximately 86 kilodaltons was immun ... | 1988 | 2831402 |
| characterization of a t-lymphocyte epstein-barr virus/c3d receptor (cd21). | the epstein-barr virus/c3d receptor (ebvr-cr2) was detected on three t-lymphoblastoid cell lines. the apparent mrs of purified ebvr-cr2 of t-cell and b-cell origin were identical. the n-terminal amino acid sequence from the t-cell ebvr-cr2 confirmed the placement of this receptor in a multigene family of complement regulatory proteins. all ebvr-cr2-positive t-cell lines were t6 and t4-t8 antigen positive. | 1988 | 2831405 |
| viruses and cancer risks: outgrowth of epstein-barr virus-positive burkitt's lymphoma in the immune host. | this work examines ways in which epstein-barr (eb) virus-positive burkitt's lymphoma (bl) cells achieve outgrowth in vivo in the face of prevailing eb virus-specific cytotoxic t-cell surveillance. earlier work has shown that some, but not all, bl cell lines in vitro are insensitive to virus-specific t-cell cytolysis and the present study identifies two mechanisms whereby the tumour cells might evade detection. first, bl-cell lines which stably retain the original tumour cell phenotype on serial ... | 1987 | 2831439 |
| t-cell lymphomas containing epstein-barr viral dna in patients with chronic epstein-barr virus infections. | fatal t-cell lymphomas developed in three patients with a chronic illness manifested by fever, pneumonia, dysgammaglobulinemia, hematologic abnormalities, and extraordinarily high titers of antibody to the epstein-barr virus (ebv) capsid antigen (greater than 10,000) and early antigen (greater than 640) but low titers to the ebv nuclear antigen (less than or equal to 40). to understand the pathogenesis of these tumors better, we determined the immunophenotype of the tumor cells and analyzed tumo ... | 1988 | 2831453 |
| intertypic cross-neutralization of polioviruses by human monoclonal antibodies. | human b cell lines producing monoclonal antibodies (mabs) reactive with poliovirus type 1 were generated by transformation with epstein-barr virus (ebv) b 95-8 of tonsillar lymphocytes from several immune donors. ebv-transformed cells were cloned in semisolid agarose. some neutralizing (nt) human mabs recognized and neutralized only poliovirus type 1, whereas other nt mabs neutralized either poliovirus type 1 and 2 or all three serotypes. mabs reactive with poliovirus type 1 and 3 but not with t ... | 1988 | 2831657 |
| comparison of tests for heterophile antibodies with a test for specific igm-antibodies to epstein-barr virus. brief report. | seventy serum samples from 54 patients with clinically suspected infectious mononucleosis were examined, using the paul-bunnell-davidsohn test, a rapid slide test and an elisa for the detection of igm antibodies against the viral capsid antigen (vca) of epstein-barr virus. the elisa technique was the most sensitive, detecting igm in 29 sera, whereas only 19 sera were positive in the paul-bunnell-davidsohn test (titre greater than or equal to 64). however, using a titre of greater than or equal t ... | 1988 | 2831918 |
| latency comes of age for herpesviruses. | 1988 | 2832064 | |
| selective induction of light chain synthesis in cultures of blood lymphocytes from patients with igg myelomatosis. | in the present study evidence is provided that neoplastic b cells from the blood from four of 24 patients with myelomatosis were activated selectively with polyclonal b cell mitogens. in three of these patients the activated cells produced light chains without heavy chains; of these, two patients had iggk paraproteins and one had free lambda light chain disease. the ratio of kappa-expressing to lambda-expressing b cells in the initial blood b cell preparations was within the range for healthy co ... | 1988 | 2832107 |
| immunoregulatory aberrations in patients with polyarticular juvenile rheumatoid arthritis. | the presence of hypergammaglobulinemia and various circulating autoantibodies in children with polyarticular juvenile rheumatoid arthritis (jra) implies an immunoregulatory disorder. we report here experiments planned to elucidate the underlying cellular aberrations in this disease. twelve children with polyarticular jra were studied. percentages of leu-1, leu-2, and leu 3 t cells were comparable to those of normal individuals. immunofluorescent double staining studies demonstrated elevated numb ... | 1988 | 2832112 |
| response of lfa-1-deficient b cells to interleukin 4 (bsf-1) and low molecular weight b cell growth factor (bcgflow). | t cell-depleted b cells from a patient with lfa-1 deficiency were tested in costimulation assays for responsiveness to recombinant human il4 (bsf-1) and purified low molecular weight b cell growth factor (bcgflow). in both cases the response of lfa-1-deficient b cells was comparable with normal controls. monoclonal antibodies to lfa-1 alpha (cd11a) and beta (cd18) chains were unable to mimic the action of il4 on normal b cells in costimulation assays with anti-igm, and did not inhibit normal b c ... | 1988 | 2832180 |