Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| analysis of dbc1 and its homologs suggests a potential mechanism for regulation of sirtuin domain deacetylases by nad metabolites. | deleted in breast cancer-1 (dbc1) and its paralog carp-1 are large multi-domain proteins, with a nuclear or perinuclear localization, and a role in promoting apoptosis upon processing by caspases. recent studies on human dbc1 show that it is a specific inhibitor of the sirtuin-type deacetylase, sirt1, which deacetylates histones and p53. using sensitive sequence profile searches and hmm-hmm comparisons we show that the central conserved globular domain present in the dbc1 and it homologs from di ... | 2008 | 18418069 |
| distribution of toxins in various tissues of crucian carp intraperitoneally injected with hepatotoxic microcystins. | an acute toxicity experiment was conducted to examine the distribution and depuration of microcystins (mcs) in crucian carp (carassius aurutus) tissues. fish were injected intraperitoneally with extracted mcs at a dose of 200 microg mc-lr (where l=leucine and r=arginine) equivalent/kg body weight. microcystin concentrations in various tissues and aquaria water were analyzed at 1, 3, 12, 24, and 48 h postinjection using liquid chromatography coupled with mass spectrometry. microcystins were detec ... | 2008 | 18419201 |
| production of nitric oxide by carp (cyprinus carpio l.) kidney leukocytes is regulated by cyclic 3',5'-adenosine monophosphate. | the inducible nitric oxide synthase (inos) plays a central role in the inflammatory reactions that follow infection or tissue damage. induction of nitric oxide (no) synthesis by bacterial lipopolysaccharide (lps) depends on activation of g protein-coupled receptors in mammals. thus, it was our intention to evaluate whether similar mechanisms are involved in inos activation in fish leukocytes. therefore, the participation of membrane-bound receptors which activate effectors via g proteins has bee ... | 2008 | 18424130 |
| [mutation rate and pattern of microsatellites in gynogenetic silver crucian carp (carassius autatus gibeblio)]. | the natural gynogenetic triploid silver crucian carp (carassius autatus gibeblio bloch) provides a good system for studying evolutional genetics of the unisexual and polyploidy vertebrate. microsatellites are abundant across genomes and show high levels of polymorphism and mutational rate, so they have been widely used for studying evolutional biology. in this study, the mutation rate and pattern at 33 microsatellite loci of silver crucian carp were investigated. as a result, it was found that t ... | 2008 | 18424420 |
| distinct cytochrome p450 aromatase isoforms in the common carp (cyprinus carpio): sexual dimorphism and onset of ontogenic expression. | cytochrome p450 aromatase (cyp19) is a key enzyme in the steroidogenic pathway that catalyses the conversion of testosterone to estrogen, and therefore is thought to influence gonadal sex differentiation. in an effort to understand the role of this enzyme in ovarian differentiation, we isolated cdna encoding the two distinct isoforms, ovarian and brain (termed cyp19a and cyp19b, respectively) of adult common carp, cyprinus carpio. the cloned cdna for cyp19a had an open reading frame (orf) of 518 ... | 2008 | 18430424 |
| application of pcr-rf-sscp to study major histocompatibility class ii b polymorphism in common carp (cyprinus carpio l.). | a variety of methods have been applied for the characterization of major histocompatibility (mh) polymorphism in fish. we optimized a technique designated polymerase chain reaction-restriction fragments-single strand conformation polymorphism (pcr-rf-sscp) for screening a large number of individuals for the cyca-dab1 and cyca-dab2 genes polymorphism in common carp. the advantages of this technique are simplicity, high sensitivity and low costs. pcr-rf-sscp analysis revealed different genotypes c ... | 2008 | 18430588 |
| intracellular cyclic-amp suppresses the permeability of gap junctions between retinal amacrine cells. | gap junctions are intercellular channels composed of subunit protein connexin and subserve electrotonic transmission between connected neurons. retinal amacrine cells, as well as horizontal cells of the same class, are homologously connected by gap junctions. the gap junctions between these neurons extend their receptive fields, and may increase the inhibitory postsynaptic effects in the retina. in the present study, we investigated whether gap junctions between the neurons are modulated by inte ... | 2008 | 18431817 |
| perfluorinated compounds in common carp (cyprinus carpio) fillets from the upper mississippi river. | ten different perfluoroalkyl acids (pfaas), including perfluooctane sulfonate (pfos), were measured in 30 common carp (cyprinus carpio) fillets collected from three sites on the upper mississippi river in minnesota in an effort to evaluate the potential impact of pfaa emissions in this area. samples upstream of the city of st. cloud (reference site) had median pfos concentrations of 8.1 ng/g wet weight (ng/g wet wt), but median levels increased significantly downstream in the minneapolis-st. pau ... | 2008 | 18439677 |
| perfluorooctane sulfonate (pfos) and other fluorochemicals in fish blood collected near the outfall of wastewater treatment plant (wwtp) in beijing. | perfluorinated compounds (pfcs) were measured in zooplankton and five fish species collected from gaobeidian lake, which receives discharge from wastewater treatment plant (wwtp) in beijing, china. the mean total pfcs in five fish were in the order: crucian carp>common carp>leather catfish>white semiknife carp>tilapia. perfluorooctane sulfonate (pfos) occurred at the greatest concentrations, with mean concentrations ranging from 5.74 to 64.2 ng/ml serum. perfluorodecanoic acid (pfda) was the sec ... | 2008 | 18439735 |
| effects of cu(2+) and pb(2+) on different fish species: liver cytochrome p450-dependent monooxygenase activities and ftir spectra. | the effects of cu(2+)-sulfate and pb(2+)-acetate on carp (cyprinus carpio l.), silver carp (hypopthalmichtys molitrix v.) and wels (silurus glanis l.) were studied. the liver microsomal cyt p450 content, the erod, ecod and apnd monooxygenase activities were measured. in vivo treatment with 1 mg l(-1) cu(2+) significantly elevated the activities of these enzymes and cyt p450 content in silver carp livers. the high-dose cu(2+) treatment (10 mg l(-1)) on silver carp caused two-fold higher induction ... | 2008 | 18439880 |
| development of microsatellite markers and their utilization in genetic diversity analysis of cultivated and wild populations of the mud carp (cirrhina molitorella). | microsatellite markers have been increasingly used in genetic studies on fishery species because of their high applicability in selective breeding programs. here we reported the development of microsatellite markers and their utilization in mud carp (cirrhina molitorella). an (ca)(15) enriched library has been constructed for mud carp, using the magnetic beads enrichment procedure. sequence analysis of 60 randomly picked positive colonies indicate that 56 (93.3%) of the colonies contain microsat ... | 2008 | 18439976 |
| novel candidate genes identified in the brain during nociception in common carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss). | recent studies have demonstrated that teleost fish possess nociceptors that detect potentially painful stimuli and that the physiological properties of these fibres are markedly similar to those found in mammals. this finding led to suggestions of possible pain perception in fish, contrary to the view that the sensory response in these animals is limited to the spinal cord and hindbrain and as such is reflexive. therefore, the aim of this study was to determine if the brain is active at the mole ... | 2008 | 18440145 |
| differential metallothionein induction patterns in fed and starved carp (cyprinus carpio) during waterborne copper exposure. | starved and fed carp (cyprinus carpio) were exposed to sublethal waterborne copper exposure (1 microm) for 28 d in softened antwerp, belgium, city tap water. copper accumulation in liver and gill tissues was determined, and changes in branchial na+/k+-adenosine 5'-triphosphatase (atpase) activity and metallothionein (mt) induction in gill and liver tissues were investigated following 28-d copper exposure. gill na+/k+-atpase activity in exposed fish, both starved and fed, was at its lowest values ... | 2008 | 18444695 |
| carp-2 is an endosome-associated ubiquitin ligase for rip and regulates tnf-induced nf-kappab activation. | the proinflammatory cytokine tumor necrosis factor-alpha (tnf-alpha) elicits cellular responses by signaling through a receptor complex that includes the essential adaptor molecule rip. one important consequence of signaling is activation of the transcription factor nf-kappab, and failure to downregulate tnf-induced nf-kappab transcriptional activity results in chronic inflammation and death. internalization of the receptor complex plays an important regulatory role in tnf signaling. | 2008 | 18450452 |
| meichroacidin containing the membrane occupation and recognition nexus motif is essential for spermatozoa morphogenesis. | meichroacidin (mca) is a highly hydrophilic protein that contains the membrane occupation and recognition nexus motif. mca is expressed during the stages of spermatogenesis from pachytene spermatocytes to mature sperm development and is localized in the male meiotic metaphase chromosome and sperm flagellum. mca sequences are highly conserved in ciona intestinalis, cyprinus carpio, and mammals. to investigate the physiological role of mca, we generated mca-disrupted mutant mice; homozygous mca mu ... | 2008 | 18453535 |
| microcystin-induced variations in transcription of gsts in an omnivorous freshwater fish, goldfish. | the glutathione s-transferases are important enzymes in the microcystin-induced detoxication processes. in this experiment, we cloned the full-length cdna of alpha, pi and theta-class-like glutathione s-transferase genes from goldfish (carassius auratus l). their derived amino acid sequences were clustered with other vertebrate alpha, pi and theta-class gsts in a phylogenetic tree and the goldfish gst sequences have the highest similarity with those from common carp and zebrafish. goldfish were ... | 2008 | 18455246 |
| influence of serotonin on the action of melatonin in mih-induced meiotic resumption in the oocytes of carp catla catla. | the influences of serotonin (5-hydroxytryptamine) on the action of melatonin (n-acetyl-5-methoxytryptamine) in mih (maturation inducing hormone)-induced meiotic resumption were evaluated in the oocytes of carp catla catla using an in vitro model. oocytes from gravid female carp were isolated and incubated separately in medium 199 containing either (a) only melatonin (mel; 100 pg/ml), or (b) only serotonin (ser; 100 pg/ml), or (c) only mih (1 microg/ml), or (d) mel and mih (e) or mel (4 h before) ... | 2008 | 18455941 |
| effects of concentrated drinking water injection on glutathione and glutathione-dependent enzymes in liver of cyprinus carpio l. | two drinking water production plants located in north italy, collecting water from the river po (plants 1 and 2) were chosen for this study. water samples were collected before and after the disinfection process and at two points along the piping system. water samples were concentrated by the solid-phase extraction system and injected intraperitoneally into specimens of cyprinus carpio. the concentration of water samples was 3 l/equiv. in order to assess the effects of the water samples on carp ... | 2008 | 18457861 |
| strongly ph-buffered ringer's solution expands the receptive field size of horizontal cells in the carp retina. | by intracellular recordings, we studied the effects of ph buffering on the size of the receptive field and the extent of dye coupling of horizontal cells (hcs) in the light-adapted carp retina. these parameters were compared between data obtained in fortified ringer's solution and those obtained in control bicarbonate ringer's of the same ph (7.60). in ringer's fortified with 10 mm hepes or 15 mm tris, the dye-coupling ratio of hcs increased by 71% and 70%, respectively. these fortified ringer's ... | 2008 | 18459824 |
| [chromosomal studies of germ cells in diploid and polyploid fish produced by distant crossing]. | by observing the chromosomal spreads of germ cells and tissue sections, we studied the chromosomal spreads of germ cells in diploid and polyploid fish produced by distant crossing. the samples covered the second generation of hybrids of red crucian carp (carassius auratus red var.) (female) x common carp (cyprinus carpio) (male) (2n = 100) (f2), allotetraploid hybrids of red crucian carp (female) x common carp (male)(4n = 200), triploid hybrids of gold fish (female)x allotetraploid (male) (3n = ... | 2008 | 18464590 |
| the complete sequence of mitochondrial genome from a gynogenetic triploid "ginbuna" (carassius auratus langsdorfi). | the complete mitochondrial (mt) genome of the gynogenetic triploid ginbuna (carassius auratus langsdorfi, az3 line) has been cloned and sequenced. the genome consisted of 16,578 bp and encoded the same set of genes (13 proteins, 2 rrnas and 22 trnas) in addition to a d-loop region, as described for other vertebrate mtdnas. comparison with other teleost mtdnas demonstrated that the protein/rrna-coding regions of the ginbuna were highly homologous both in length and nucleotide composition to those ... | 1998 | 18465995 |
| dietary phosphatidylcholine affects postprandial plasma levels and digestibility of lipid in common carp (cyprinus carpio). | this study examined the effect of purified soyabean phosphatidylcholine (spc) on circulating plasma lipids and nutrient digestibility in juvenile common carp. the fish (100 (sd 15) g, 25 degrees c) were fed, for 4 weeks, a casein-based diet containing either 12 % soyabean oil (diet sbo) or 8 % sbo plus 4 % spc (diet spc). the lipid, protein and energy contents of the faeces were analysed for the determination of apparent digestibility. at the end of the trial, the fish were fasted for 48 h and f ... | 2008 | 18466656 |
| expression of an adenovirus encoded reporter gene and its reactivation following uvc and oxidative damage in cultured fish cells. | recombinant human adenovirus, adca35lacz, was used to examine expression of a reporter gene and its reactivation following uvc (200-280 nm) and oxidative damage in fish cells. | 2008 | 18470745 |
| extensive expansion and diversification of the chemokine gene family in zebrafish: identification of a novel chemokine subfamily cx. | the chemokine family plays important roles in cell migration and activation. in humans, at least 44 members are known. based on the arrangement of the four conserved cysteine residues, chemokines are now classified into four subfamilies, cxc, cc, xc and cx3c. given that zebrafish is an important experimental model and teleost fishes constitute an evolutionarily diverse group that forms half the vertebrate species, it would be useful to compare the zebrafish chemokine system with those of mammals ... | 2008 | 18482437 |
| family association between immune parameters and resistance to aeromonas hydrophila infection in the indian major carp, labeo rohita. | seven innate immune parameters were investigated in 64 full-sib families (the offspring of 64 sires and 45 dams) from two year-classes of farmed rohu carp (labeo rohita). survival rates were also available from aeromonas hydrophila infection (aeromoniasis) recorded in controlled challenge tests on a different sample of individuals from the same families. due to strong confounding between the animal additive genetic effect and the family effects (common environmental+non-additive genetic), reliab ... | 2008 | 18486488 |
| [correlation analysis of microsatellite dna markers with body weight, length and height of common carp (cyprinus carpio l.)]. | forty-seven microsatellite markers were selected to analyze the genomic dna of 92 progenies derived from the recombinant inbred lines (ril) of common carp, which came from the cross between barbless carp and hebao-cold tolerance red carp. the results showed that a total of 162 different alleles were found, and the number of alleles in each locus was 2 to 6. the dna fragment length was 100 bp to 444 bp, and the number of mean valid alleles was 1.3069 to 4.2288. the value of heterozygosity was 0.2 ... | 2008 | 18487152 |
| formation and biological characterization of three new types of improved crucian carp. | the improved tetraploids (g(1)xat) were obtained by distant crossing and gynogenesis and the high-body individuals accounted for 2% among g(1)xat. after mating with each other, the high-body individuals produced three kinds of bisexual fertile diploid fishes: high-body red crucian carp, high-body fork-like-tails goldfish and gray common carp. the high-body red crucian carp mating with each other formed three types of improved crucian carp (icc) including improved red crucian carp (ircc), improve ... | 2008 | 18488175 |
| protein expression by aeromonas hydrophila during growth in vitro and in vivo. | expression of aeromonas hydrophila cellular and extracellular products (ecps) was examined following culture of the bacterium in vitro, in tryptic soy broth (tsb), and in vivo, in dialysis tubing placed within the peritoneal cavity of common carp (cyprinus carpio l.). whole cell (wc), outer membrane proteins (omps) and ecp components of the bacteria were analysed by 1 dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (1d sds-page). additionally, 2d sds-page was used to analy ... | 2008 | 18490132 |
| recombinant novel pituitary adenylate cyclase-activating polypeptide from african catfish (clarias gariepinus) authenticates its biological function as a growth-promoting factor in low vertebrates. | nowadays, the studies of pituitary adenylate cyclase-activating polypeptide (pacap)-related peptide (prp) and pacap in non-mammalian vertebrates, especially in fish, have paid attention mainly to the localization, cloning, and structural evolution of the peptides, but very little is known about its biological functions as growth-promoting factors in low vertebrates. in this work, we have cloned and characterized the prp/pacap cdna from the commercially important north african catfish clarias gar ... | 2008 | 18492822 |
| natural hybridization between diploid crucian carp species and genetic independence of triploid crucian carp elucidated by dna markers. | the population structure of genus carassius in lake koyama, southeast japan, was analyzed by genetic markers as microsatellite dna, mtdna rflp and isozymes. based on the ploidy level and morphological analysis, four carassius groups were detected. the triploid group was identified as ginbuna (c. langsdorfii). in the diploid group, nagabuna (c. burugeri sp) and gengoroubuna, (c. cuvieri) were identified. remaining diploid individuals had morphological traits that were intermediate between nagabun ... | 2000 | 18494591 |
| nitric oxide formation from the reaction of nitrite with carp and rabbit hemoglobin at intermediate oxygen saturations. | the nitrite reductase activity of deoxyhemoglobin has received much recent interest because the nitric oxide produced in this reaction may participate in blood flow regulation during hypoxia. the present study used spectral deconvolution to characterize the reaction of nitrite with carp and rabbit hemoglobin at different constant oxygen tensions that generate the full range of physiological relevant oxygen saturations. carp is a hypoxia-tolerant species with very high hemoglobin oxygen affinity, ... | 2008 | 18494799 |
| enhancement of ultrasonically induced cell damage by phthalocyanines in vitro. | in this work, erythrocytes from carp were used as a nucleated cell model to test the hypothesis that the phthalocyanines (zinc--znpc and chloroaluminium -alclpc) enhance ultrasonically induced damage in vitro. in order to confirm and complete our earlier investigation, the influence of ultrasound (us) and phthalocyanines (pcs) on unresearched cellular components, was studied. red blood cells were exposed to 1 mhz continuous ultrasound wave (0.61 and/or 2.44 w/cm(2)) in the presence or absence of ... | 2008 | 18495194 |
| analysis of sulfamerazine in pond water and several fishes by high-performance liquid chromatography using molecularly imprinted solid-phase extraction. | the synthesis and evaluation of a molecularly imprinted polymer (mip) used as a selective solid-phase extraction sorbent and coupled to high-performance liquid chromatography (hplc) for the efficient determination of sulfamerazine (smr) in pond water and three fishes are reported. the polymer was prepared using smr as the template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the crosslinking monomer in the presence of tetrahydrofuran as the solvent. ... | 2008 | 18496678 |
| the complete mitochondrial genome of rock carp procypris rabaudi (cypriniformes: cyprinidae) and phylogenetic implications. | rock carp, procypris rabaudi (tchang), is an endemic fish species in china. we sequenced the complete mitochondrial genome of it by high-fidelity polymerase chain reaction with conserved primers and primer walking sequencing method. the complete mitochondrial genome of rock carp is 16595 bp in length and contains 13 protein-coding genes, two ribosomal rna (rrna) genes, 22 transfer rna (trna) genes and one control region, with an identical order to that of most other vertebrates. the origin of l- ... | 2009 | 18496768 |
| toxicity evaluation of perfluorooctane sulfonate (pfos) in the liver of common carp (cyprinus carpio). | perfluorooctane sulfonate (pfos) has been manufactured for over 50 years in increasing quantities and has been used for several industrial and commercial aims. due to persistence and bioaccumulation of this pollutant, it can be found worldwide in wildlife and humans. biochemical effects of pfos exposure are mainly studied in mammalian model species and information about effects on fish species remain largely scarce. this lack of toxicity data points out that there is an urgent need for the mecha ... | 2008 | 18501439 |
| transcriptional analysis of the common carp (cyprinus carpio l.) immune response to the fish louse argulus japonicus thiele (crustacea: branchiura). | in the present study we investigated changes in transcription levels of a panel of selected immune relevant genes in peripheral blood leucocytes (pbl) and skin samples collected from carp exposed to larval argulus japonicus. we show that in skin up-regulation of gene transcription of the chemokine cxca, and to a lesser extent the chemokine receptor cxcr1 and the cytokine tnfalpha, are good indicators of parasite-induced skin damage at 2 days post-parasite exposure. up-regulation of gene transcri ... | 2008 | 18502149 |
| cdna expression library screening and identification of two novel antigens: ubiquitin and receptor for activated c kinase (rack) homologue, of the fish parasite trypanosoma carassii. | trypanosoma carassii is a kinetoplastid parasite infecting cyprinid fish with a high prevalence in nature. antibodies have been shown to play a protective role in the immune response against this parasite in common carp, cyprinus carpio. to identify immunogenic and putative protective t. carassii antigens we constructed a lambdatriplex2 expression library of the parasite and screened this with pooled carp immune serum collected 6 weeks post-infection. screening of the library not only revealed r ... | 2008 | 18502151 |
| comparison of natural and recombinant forms of the major fish allergen parvalbumin from cod and carp. | allergic reaction following fish consumption can trigger life-threatening reactions in predisposed individuals. parvalbumins from different species have been identified as the major fish allergens. there are two distinct phylogenetic lineages of parvalbumins, alpha and beta. most allergic reactions are caused by beta-parvalbumins. we cloned and expressed cdnas encoding cod (gadus morhua) and carp (cyprinus carpio) beta-parvalbumins and purified natural cod beta-parvalbumin. cd spectra of the pur ... | 2008 | 18504705 |
| corticosteroid receptors involved in stress regulation in common carp, cyprinus carpio. | in higher vertebrates, mineralo- (aldosterone) and glucocorticoids (cortisol/corticosterone) exert their multiple actions via specific transcription factors, glucocorticoid (gr) and mineralocorticoid (mr) receptors. teleostean fishes lack aldosterone and mineral regulatory processes seem under dominant control by cortisol. despite the absence of the classical mineralocorticoid aldosterone, teleostean fishes do have an mr with cortisol and possibly 11-deoxycorticosterone (doc) (as alternative for ... | 2008 | 18505847 |
| hypoxia inhibits fish spawning via lh-dependent final oocyte maturation. | to evaluate the effects of long term hypoxia exposure on fish spawning, mature common carp, cyprinus carpio carpio (linnaeus) were subjected to either normoxia (7.4+/-0.2 mgo(2)mg o(2) l(-1)) or hypoxia (1.0+/-0.2 mgo(2)o(2) l(-1)) for more than two months. gonadosomatic index (gsi), and concentrations of serum luteinizing hormone (lh), testosterone (t), and estroldiol (e2) were measured and gonad histology examined. hypoxia inhibits fish spawning even though the gonad and oocytes developed unde ... | 2008 | 18508413 |
| rod and cone photoreceptors: molecular basis of the difference in their physiology. | vertebrate retinal photoreceptors consist of two types of cells, the rods and cones. rods are highly light-sensitive but their flash response time course is slow, so that they can detect a single photon in the dark but are not good at detecting an object moving quickly. cones are less light-sensitive and their flash response time course is fast, so that cones mediate daylight vision and are more suitable to detect a moving object than rods. the phototransduction mechanism was virtually known by ... | 2008 | 18514002 |
| responses of biochemical markers in carp cyprinus carpio from two field sites in western ukraine. | the aim of this study was to compare environmental quality in two sites of the river in western ukraine, rural (r) and industrial (i) during three seasons via a set of biochemical markers in carp cyprinus carpio l. upon comparing the values of the i-site with those of the r-site, we found that mn- and cu, zn-superoxide dismutase (sod) activities decreased and o(.) production increased; metallothionein (mt) and glutathione levels increased in most cases, and acetylcholinesterase (ache) activity d ... | 2009 | 18514900 |
| immunohistochemical localization of c-rfamide, a fmrf-related peptide, in the brain of the goldfish, carassius auratus. | carassius rfamide (c-rfa) is a novel peptide found in the brain of the japanese crucian carp. it has been demonstrated that mrna of c-rfa is present in the telencephalon, optic tectum, medulla oblongata, and proximal half of the eyeball in abundance. immunohistochemical methods were employed to elucidate the distribution of the peptide in the brain of the goldfish (carassius auratus) in detail. c-rfaimmunoreactive perikarya were observed in the olfactory bulb, the area ventralis telencephali par ... | 2000 | 18522460 |
| antibody response and resistance of cyprinus carpio immunized with cyprinid herpes virus 3 (cyhv-3). | cyhv-3 (cyprinid herpesvirus-3) is a large dna virus that causes a fatal disease in koi and common carp. infection with wild type or attenuated virus induces an immune response that renders the fish resistant to further virus challenges. the kinetics and affinity of the antibody response in immune fish depend on the temperature of the water. virus-inoculated fish produce anti-cyhv-3 antibodies, which gradually decrease during 280 days post infection to a level slightly above that of naïve fish. ... | 2008 | 18534721 |
| carp vitellogenin detection by an optical waveguide lightmode spectroscopy biosensor. | a label-free carp vitellogenin sensor has a strong potential for on-site monitoring on the possible contamination of edible fish with endocrine disruptors as a sum parameter in an inland carp farm. in this study, we performed a sensitive detection for carp vitellogenin with a direct-binding optical waveguide lightmode spectroscopy-based immunosensor. carp vitellogenin bound over the sensor surface quite specifically, judging from the sensor responses according to stepwise antibody immobilization ... | 2008 | 18534837 |
| globins and hypoxia adaptation in the goldfish, carassius auratus. | goldfish (carassius auratus) may survive in aquatic environments with low oxygen partial pressures. we investigated the contribution of respiratory proteins to hypoxia tolerance in c. auratus. we determined the complete coding sequence of hemoglobin alpha and beta and myoglobin, as well as partial cdnas from neuroglobin and cytoglobin. like the common carp (cyprinus carpio), c. auratus possesses two paralogous myoglobin genes that duplicated within the cyprinid lineage. myoglobin is also express ... | 2008 | 18537818 |
| serum crp-like protein profile in common carp cyprinus carpio challenged with aeromonas hydrophila and escherichia coli lipopolysaccharide. | the potential of c-reactive protein (crp)-like proteins to be used as a biomarker of health status in cultured carp obtained from various european fish lines has been assessed. varying crp-like protein levels in the serum of carp were monitored using an indirect competitive enzyme-linked immunosorbent assay. crp-like protein basal levels in normal fish varied between carp lines, ranging on average from 2.9+/-0.15 to 12.57+/-1.19 microg ml(-1). serum levels of crp-like protein in carp were observ ... | 2008 | 18538390 |
| teleostean il11b exhibits complementing function to il11a and expansive involvement in antibacterial and antiviral responses. | interleukin 11 is a class-1 helical cytokine, having the four-helix bundle structure, possessing pleiotropic characteristics involved in physiological processes including blood production, bone formation and placentation. the interleukin 11 paralogues (il11a and il11b) have been identified in fish with only il11a from carp and trout have been characterized and analyzed for its expression thus far. here, we cloned and studied the structure and expression of il11b in japanese flounder (paralichthy ... | 2008 | 18538848 |
| a novel inwardly rectifying k+ channel, kir2.5, is upregulated under chronic cold stress in fish cardiac myocytes. | a new member of the inward-rectifier k(+) channel subfamily kir2 was isolated and characterised from the crucian carp (carassius carassius) heart. when expressed in cos-1 cells this 422 amino acid protein produced an inward-rectifying channel with distinct single-channel conductance, mean open time and open probability. phylogenetic sequence comparisons indicate that it is not homologous to any known vertebrate kir channel, yet belongs to the kir2 subfamily. this novel crucian carp channel incre ... | 2008 | 18552306 |
| expression profiles of matrix metalloproteinase 9 in teleost fish provide evidence for its active role in initiation and resolution of inflammation. | matrix metalloproteinase 9 (mmp-9) belongs to a family of zinc-dependent endopeptidases. as a consequence of its ability to cleave structural extracellular matrix molecules, mammalian mmp-9 is associated with vital inflammatory processes such as leucocyte migration and tissue remodelling and regeneration. interestingly, mmp-9 genes have been identified in fish, but functional data are still limited and focus on the involvement of mmp-9 in embryonic development, reproduction and post-mortem tende ... | 2008 | 18557954 |
| effect of long-term exposure to simazine on histopathology, hematological, and biochemical parameters in cyprinus carpio. | we investigated if residues of simazine in the natural waters would cause histological, hematological, and biochemical alterations in carps from contaminated areas in badajoz (spain). some necrotic foci in kidney and liver, hepatitis, and hepatic steatosis were detected. no changes on measured hematological and biochemical parameters between fish from reference and contaminated ponds were observed. to assess if simazine exposure was the cause of these observations carps were exposed in the labor ... | 2009 | 18561303 |
| trypanoplasma borreli cysteine proteinase activities support a conservation of function with respect to digestion of host proteins in common carp. | trypanoplasma borreli is an extracellular parasite that is transmitted by a leech vector and is naturally found in the blood of cyprinid fish. high parasitemia and associated severe anemia together with splenomegaly are typical of infection of common carp, cyprinus carpio l. papain-like cysteine proteinases expressed by trypanosome parasites contribute to the pathogenicity of trypanosomes, and are considered an important target for the development of new trypanocidal drugs. t. borreli is a membe ... | 2008 | 18571233 |
| adapting a markov monte carlo simulation model for forecasting the number of coronary artery revascularisation procedures in an era of rapidly changing technology and policy. | treatments for coronary heart disease (chd) have evolved rapidly over the last 15 years with considerable change in the number and effectiveness of both medical and surgical treatments. this period has seen the rapid development and uptake of statin drugs and coronary artery revascularization procedures (carps) that include coronary artery bypass graft procedures (cabgs) and percutaneous coronary interventions (pcis). it is difficult in an era of such rapid change to accurately forecast requirem ... | 2008 | 18578858 |
| effect of anoxia on the electroretinogram of three anoxia-tolerant vertebrates. | to survive anoxia, neural atp levels have to be defended. reducing electrical activity, which accounts for 50% or more of neural energy consumption, should be beneficial for anoxic survival. the retina is a hypoxia sensitive part of the central nervous system. here, we quantify the in vivo retinal light response (electroretinogram; erg) in three vertebrates that exhibit varying degrees of anoxia tolerance: freshwater turtle (trachemys scripta), epaulette shark (hemiscyllium ocellatum) and leopar ... | 2008 | 18579424 |
| the optimization of the protocol for immunofluorescence on fish spermatozoa. | in comparison with mammals, the fertilization of fish occurs predominantly outside the organism in a water environment, where fish spermatozoa require specific conditions to interact with oocytes. it is evident that optimal conditions for fish and mammalian spermatozoa are quite different. this paper describes a special approach to handling fish (common carp and siberian sturgeon) spermatozoa in comparison with the samples originating from mammals (boar). this approach concerns not only the diff ... | 2008 | 18584860 |
| cardiac survival in anoxia-tolerant vertebrates: an electrophysiological perspective. | certain vertebrates, such as freshwater turtles of the genus chrysemys and trachemys and crucian carp (carassius carassius), have anoxia-tolerant hearts that continue to function throughout prolonged periods of anoxia (up to many months) due to successful balancing of cellular atp supply and demand. in the present review, we summarize the current and limited understanding of the cellular mechanisms underlying this cardiac anoxia tolerance. what emerges is that cold temperature substantially modi ... | 2008 | 18589002 |
| expression of genes involved in excitatory neurotransmission in anoxic crucian carp (carassius carassius) brain. | the crucian carp, carassius carassius, survives months without oxygen. during anoxia it needs to keep energy expenditure low, particularly in the brain, with its high rate of atp use related to neuronal activity. this could be accomplished by reducing neuronal excitability through altered expression of genes involved in excitatory neurotransmission. through cloning and the use of a recently developed real-time rt-pcr approach, with an external rna control for normalization, we investigated the e ... | 2008 | 18593861 |
| identification and characterization of two mtf-1 genes in common carp. | the metal-responsive transcription factor (mtf-1) serves as an essential regulator of zn(2+) homeostasis via the activation of metallothionein gene expression. only a single mtf-1 gene has been identified in any organism investigated previously. we report here the first evidence of the existence of two genes encoding mtf-1 proteins (mtf-1.1 and mtf-1.2). the expression patterns were followed in the liver, kidney, muscle, brain and heart by means of northern hybridization and reverse transcriptio ... | 2008 | 18602497 |
| effect of botulinum toxin a-induced paralysis and exercise training on mechanosensing and signalling gene expression in juvenile rat gastrocnemius muscle. | intramuscular injections of the paralytic botulinum neurotoxin a (btx) and physical exercise are used in the treatment of chronic spasticity in children with cerebral palsy. we tested whether btx-induced paralysis and/or exercise training would have differential effects on the expression of mechanosensing and signalling genes implicated in the adaptive remodelling of skeletal muscle. juvenile (29-day-old) male rats were injected with btx or saline (nobtx) into the right gastrocnemius and housed ... | 2008 | 18603602 |
| distribution of marine birnavirus in cultured olive flounder paralichthys olivaceus in korea. | surveys of marine birnavirus (mabv) were undertaken in cultured olive flounder paralichthys olivaceus from the south and west coastal areas and jeju in korea during the period january 1999 to april 2007. mabv was detected in all seasons from the fry, juveniles and adult fish from the areas examined. evident cytopathic effects of the virus including rounding and cell lysis were observed in chinook salmon embryo (chse-214) and rainbow trout gonad (rtg-2) cells, but not in fathead minnow (fhm) and ... | 2008 | 18604495 |
| comparative study on the morphology and the composition of the otoliths in the teleosts. | saccular otoliths of teleosts were mostly larger than utricular otoliths, which might relate to the three-dimensional movement. the large and heavy otolith may be better suited in saccules of the bottom and reef fishes. the quantities of iron in lagenar otoliths were found to be lower than those of birds. the function of the fish lagena remains to be elucidated by further studies. | 2008 | 18607888 |
| mmts, a new subfamily of tc1-like transposons. | a novel tc1-like transposable element has been identified as a new dna transposon in the mud loach, misgurnus mizolepis. the m. mizolepis tc1-like transposon (mmts) is comprised of inverted terminal repeats and a single gene that codes tc1-like transposase. the deduced amino acid sequence of the transposase-encoding region of mmts transposon contains motifs including dde motif, which was previously recognized in other tc1-like transposons. however, putative mmts transposase has only 34-37% ident ... | 2008 | 18612245 |
| neuropeptides and monoamines in the carp (cyprinus carpio) pretectum: an immunocytochemical study. | the distribution of neurotensin, neurokinin a, dynorphin a, galanin, somatostatin-28 (1-12), neuropeptide y, vasoactive intestinal polypeptide, gastrin-releasing peptide, gamma-melanocyte stimulating hormone, alpha-neo-endorphin, angiotensin h, cholecystokinin-8, serotonin and tyrosine hydroxylase has been studied in the pretectal nuclei of the cyprinus carpio: nuclei pretectalis superficialis parvicellularis and magnocellularis, pretectalis centralis, pretectalis, and pretectalis periventricula ... | 1993 | 18621246 |
| modulation of fenthion-induced oxidative effects by bso in the liver of cyprinus carpio l. | the objective of the present study was to evaluate the oxidative stress potential of low-level organophosphate fenthion exposure with the modulatory effect of buthionine sulfoximine in the liver of cyprinus carpio l. the fish were exposed to 20% of 96-hour lc(50) of fenthion for 24 and 96 hours. total and oxidized glutathione, thiobarbituric acid reactive substances, protein levels, glutathione peroxidase, glutathione reductase, glutathione-s-transferase, superoxide dismutase, and catalase-speci ... | 2008 | 18622870 |
| [larsen syndrome: two reports of cases with spinal cord compromise]. | larsen syndrome is characterised by untreatable congenital dislocation of multiple body joints, along with marked foot deformities. these patients have a flattened face with a short nose, a broad depressed nasal bridge and a prominent forehead. in this clinical note our aim is to report two cases that coursed with spinal cord compromise. | 2016 | 18623006 |
| rodlet cells in the epidermis of fish exposed to stressors. | rodlet cells (rc) were detected in the epidermis of carp (cyprinus carpio) and trout (oncorhynchus mykiss) exposed to stressors (e.g. acid water, heavy metals, thermal elevation, polluted water of the rhine river, water loaded with organic manure, and distilled water), as well as after wounding. these cells were not found in the epidermis of fish kept under control conditions, thus suggesting that the appearance of rc in the epidermis is stressor-related. immature rc, resembling mucous cells, we ... | 1997 | 18627825 |
| development and evaluation of a one-step loop-mediated isothermal amplification for detection of spring viraemia of carp virus. | spring viraemia of carp virus (svcv) is the causative agent of svc disease. the main aim of our study was to develop a one-step reverse transcription loop-mediated isothermal amplification (rt-lamp) assay for rapid, sensitive and effective detection of svcv. | 2008 | 18631262 |
| effects of cyanobacterial extracts containing anatoxin-a and of pure anatoxin-a on early developmental stages of carp. | this study compares the effects of pure anatoxin-a and cyanobacterial extracts of an anatoxin-a producing strain on early stages of development of carp. carp eggs were exposed from 2:30 h to 4 days post-fertilization to different ecologically relevant concentrations of anatoxin-a, provided as pure toxin or contained in the cyanobacterial extracts. data on time to mortality, mortality rate, time to hatching, hatching rate, skeletal malformations rate, and larval standard length were registered un ... | 2009 | 18640721 |
| bioaccumulation of hexachlorocyclohexane, dichlorodiphenyltrichloroethane, and estradiol-17beta in catfish and carp during the pre-monsoon season in india. | this investigation was performed to monitor hexachlorocyclohexane isomers (hchs), dichlorodiphenyltrichloroethane (ddt, and its metabolites, refered to as ddts), plasma levels of estradiol-17beta (e2), and the gonadosomatic index (gsi) between sampling sites of unpolluted ponds of gujartal, jaunpur (control site) and the polluted rivers gomti (jaunpur) and ganga (varanasi), which affect the reproductive physiology of some edible catfish and carp during the pre-monsoon season. hchs and ddts were ... | 2008 | 18649020 |
| seasonal morphological and biochemical changes of dahlgren cells implies a potential role of the caudal neurosecretory system (cnss) in the reproduction cycle of teleostean fish. | the purpose of this mini-review is to summarize recent research on the seasonal morphological and biochemical changes of dahlgren cells in the caudal neurosecretory system (cnss) of the freshwater teleosts carp carassius auratus. the quantitative proof for these seasonal changes in the morphology and biochemistry of dahlgren cells reflects the relationship between the cnss and the reproduction cycle of fish and implies that the cnss is probably involved in the reproduction process of fish. | 2008 | 18649021 |
| mechanism of cd2+ on dna cleavage and ca2+ on dna repair in liver of silver crucian carp. | the subject of acute injury, apoptosis and canceration of animals induced by heavy metal ions has been one of the hotspots studied worldwide. however, the exact molecular mechanism of cd-induced carcinogenicity remains largely unclear, and how to relieve the toxicity in vivo has rarely been reported. for this paper, we have investigated the mechanism of cd2+ on dna cleavage and ca2+ on dna repair in the liver of silver crucian carp (carassius auratus gibelio) by agarose gel electrophoresis metho ... | 2008 | 18649022 |
| [effectiveness and characteristics of treating algae-laden raw water by stocking silver carp]. | to reduce the negative effect of algae on conventional water treatment, a full-scale research of removing algae from algae-laden raw water by stocking filter-feeding silver carp was processed. after the pretreatment in a presedimentation tank with silver carp, the concentration of phytoplankton, the biomass of cyanobacteria and microsystis flos-aquae in algae-laden raw water with microsystis flos-aquae its dominant species decreased 61.8%, 76.1% and 78.2% respectively. this effective decrease of ... | 2008 | 18649519 |
| effects of a phase iii cardiac rehabilitation program on physical status and lipid profiles in elderly patients with coronary artery disease: juntendo cardiac rehabilitation program (j-carp). | limited data are available regarding the effects of phase iii cardiac rehabilitation on the physical status and risk factors in elderly patients with coronary artery disease (cad). | 2008 | 18654005 |
| effects of subchronic exposure to lasso mtx (alachlor 42% w/v) on hematological indices and histology of the common carp, cyprinus carpio l. | the aim of the study was to evaluate subchronic toxic effects of the preparation lasso mtx (alachlor 42% w/v) on hematological indices and histology of the common carp (cyprinus carpio l.). in carp exposed for 28 days to lasso mtx in the concentration of 2,400 microg l(-1), significant differences (p < 0.05), were detected in all indices of the erythrocyte profiles tested except hematocrit (pcv) when compared to controls. at a lower exposure (240 microg l(-1)) concentration of blood hemoglobin a ... | 2008 | 18654729 |
| expression of cardiac ankyrin repeat protein, carp, in malignant tumors: diagnostic use of carp protein immunostaining in rhabdomyosarcoma. | cardiac ankyrin repeat protein (carp) is highly expressed in cardiac muscles and detectable in normal skeletal muscles. arpp, a close homolog of carp, has been demonstrated to be useful for distinguishing rhabdomyosarcoma from other malignant tumors. however, the carp distributions among malignant tumors have been poorly investigated. here, we analyzed the comprehensive expression of carp in malignant tumors and evaluated its potential use for rhabdomyosarcoma diagnosis. a total of 159 malignant ... | 2008 | 18656235 |
| differential expression of two interferon-gamma genes in common carp (cyprinus carpio l.). | two interferon gamma (ifn-gamma) genes are expressed in immune cells of teleost fish and are potentially implicated in b- and t-lymphocyte responses. ifn-gamma-2 shows structural and functional characteristics to other vertebrate ifn-gamma genes and is associated with t-lymphocyte function. expression profiling shows ifn-gamma-2 upregulation in t-lymphocytes after phytohemagglutinin (pha) stimulation in vitro. unexpectedly, we found ifn-gamma-1, which is structurally different from ifn-gamma-2, ... | 2008 | 18657572 |
| the goldfish (carassius auratus) as a model for neuroendocrine signaling. | goldfish (carassius auratus) are excellent model organisms for the neuroendocrine signaling and the regulation of reproduction in vertebrates. goldfish also serve as useful model organisms in numerous other fields. in contrast to mammals, teleost fish do not have a median eminence; the anterior pituitary is innervated by numerous neuronal cell types and thus, pituitary hormone release is directly regulated. here we briefly describe the neuroendocrine control of luteinizing hormone. stimulation b ... | 2008 | 18657592 |
| qualitative parameters of the piapara semen (leporinus elongatus valenciennes, 1850). | qualitative parameters of piapara semen (leporinus elongatus) were evaluated before and after hormonal induction with carp pituitary extract at 2.5 mg.kg(-1) of live weight. the progressive motility, the spermatic vigor and the lifetime of the spermatozoa were higher before the hormonal induction (p > 0.05). the percentage of normal spermatozoa and spermatozoa with secondary pathologies did not differ (p > 0.05) between treatments: before induction (44.0 and 44.4%, respectively) and after-induct ... | 2008 | 18660966 |
| neurosecretory neurons of the nucleus preopticus (npo) express salmon gnrh mrna and show reproduction phase-related variation in the female indian major carp, cirrhinus cirrhosus. | we studied the expression of sgnrh mrna in the neurons of the nucleus preopticus (npo) of the indian major carp, cirrhinus cirrhosus, and their correlation with the reproductive status of the fish. non-radioisotopic in situ hybridization histochemistry protocol employing biotinylated-oligonucleotide probes complementary to salmon gnrh, cichlid gnrh i, catfish gnrh, chicken gnrh ii (from cichlid and catfish), and mammalian gnrh, were applied to the sections through the poa of the female indian ma ... | 2008 | 18664387 |
| physiological responses of over-wintering common carp (cyprinus carpio) to disturbance by eurasian otter (lutra lutra). | using a tame animal, the impact of otter (lutra lutra) disturbance on over-wintering carp (cyprinus carpio) was monitored in two experiments, 133 and 140 days, respectively, over two consecutive winters (november-april). the level of stress in over-wintering carp exposed to various intensities of disturbance by otters was quantified using biological indicators of stress (cortisol, cortisone, indices of nitrogen, carbohydrate, lipid and mineral metabolism and activity of basic blood plasma enzyme ... | 2008 | 18665460 |
| use of vitamin c as an immunostimulant. effect on growth, nutritional quality, and immune response of labeo rohita (ham.). | teleost fish lack the enzyme for endogenous synthesis of ascorbic acid (aa), an essential micronutrient for fish. the aim of this study was to examine the effect of higher levels of dietary vitamin c on growth, nutritional quality, and immunomodulation in the indian major carp, rohu (labeo rohita). four groups of l. rohita were fed experimental diets containing either no vitamin c (control) or supplemented with vitamin c at 500 mg kg(-1) (exp-1), 1000 mg kg(-1) (exp-2), or 1500 mg kg(-1) (exp-3) ... | 2008 | 18665463 |
| differential host utilisation by different life history stages of the fish ectoparasite argulus foliaceus (crustacea: branchiura). | in this study we examine differences in the occurrence of life history stages of the destructive fish ectoparasite argulus foliaceus (l., 1758) on eight fish species (stickleback, rudd, roach, gudgeon, bream, tench, crucian carp and common carp) sampled from a mixed-species recreational fishing lake on nine occasions during late spring and summer. total numbers ofa. foliaceus, as well as the number of larval, juvenile and adult parasite stages, from each fish were recorded along with the fish sp ... | 2008 | 18666418 |
| in vivo characterization of primitive hematopoietic cells in clonal ginbuna crucian carp (carassius auratus langsdorfii). | primitive hematopoietic cells in mammalian bone marrow are purified by flow cytometry using hoechst 33342 (hoechst) and rhodamine-123 (rho), because these dyes efflux activities of hematopoietic cells widely conserved in mammals. hematopoietic stem cells (hscs) are identified as side population (sp) cells, characterized by specific hoechst efflux pattern in flow cytometric analysis. we previously demonstrated that sp cells from teleost body kidney (bk) had the hsc activity by a transplantation e ... | 2008 | 18674821 |
| tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (carassius carassius) intraperitoneally injected at a sublethal dose. | an acute toxicity experiment was conducted by intraperitoneal injection with a sublethal dose of extracted microcystins (mcs), 50 microg mc-lr (where l = leucine and r = arginine) equivalent/kg body weight (bw), to examine tissue distribution and depuration of mcs in crucian carp (carassius carassius). liver to body weight ratio increased at 3, 12, 24, and 48 h postinjection compared with that at 0 h (p < 0.05). mc concentrations in various tissues and aquaria water were analyzed at 1, 3, 12, 24 ... | 2008 | 18677427 |
| infection of the heart of the common bream, abramis brama (l.), with myxobolus s.l. dogieli (myxozoa, myxobolidae). | myxobolus dogieli bykhovskaya-pavlovskaya & bykhovski, 1940 is regarded as a site specific myxosporean, infecting the heart of cyprinid fish. during a survey of the myxosporean fauna of lake balaton fish, heart myxobolosis was found in the common bream, abramis brama, with heavy infection of the ventricle and the bulbus arteriosus in some infected bream. developing and mature plasmodia were mostly in the connective tissue of the subepicardium and subendocardium. plasmodia developing in the suben ... | 2008 | 18700938 |
| effect of prebiotic xylooligosaccharides on growth performances and digestive enzyme activities of allogynogenetic crucian carp (carassius auratus gibelio). | the effect of prebiotic xylooligosaccharides (xos) on the growth performance and digestive enzyme activities of the allogynogenetic crucian carp, carassius auratus gibelio, was investigated. xos was added to fish basal semi-purified diets at three concentrations by dry feed weight: diet 1, 50 mg kg(-1); diet 2, 100 mg kg(-1); diet 3, 200 mg kg(-1), respectively. twelve aquaria (n = 20) with three replicates for each treatment group (diets 1-3) and control treated without xos were used. weights o ... | 2009 | 18704733 |
| characterisation of cdnas of key genes involved in apoptosis in common carp (cyprinus carpio l.). | apoptosis is a morphologically and biochemically distinct form of eukaryotic cell death that occurs under a variety of physiological and pathological conditions. many of the cell deaths that occur during normal embryological development and during normal tissue turnover display the morphological hallmarks of apoptosis. in the last 20 or so years a better biochemical picture of how the process occurs has been produced, at least in higher vertebrates. it is now widely accepted that many of the pro ... | 2008 | 18707005 |
| [inactivation of amp-deaminase from white muscle of cyprinus carpio in the systems with free radical oxidation]. | the purification and in vitro inactivation of amp-deaminase from white muscle of carp cyprinus carpio were conducted in the fe2+/h2o2 and fe2+/ascorbate oxidation systems. the enzyme activity decreases by 50% within 30 minutes of incubation in the presence of 100 microm of hydrogen peroxide and 5 microm of ferrous sulfate. inactivation depended on incubation time and concentrations of feso4 and h2o2. in the system fe2+/ascorbate the enzyme activity decreased by 50% at concentration of ascorbate ... | 2015 | 18712110 |
| a kinetic model for the relative contribution of waterborne and dietary cadmium and zinc in the common carp (cyprinus carpio). | in this work, the uptake and whole-body accumulation of cd and zn by the common carp from water, diet, and a combination of both was studied using the radioactive tracers 09cd and 65zn. a three-compartment pharmacokinetic model was constructed and revealed metal- and exposure route-dependent accumulation profiles. under the tested exposure conditions in water (0.1 microm cd and 1 microm zn), only 0.1 and 0.07%, respectively, of the total cd and zn load that passed the branchial surfaces was accu ... | 2009 | 18712945 |
| histopathological and ultrastructural features of koi herpesvirus (khv)-infected carp cyprinus carpio, and the morphology and morphogenesis of khv. | epizootics of koi herpesvirus (khv) cause mass mortalities in koi carp and common carp worldwide. we used a newly developed 'per-gill infection' procedure with live khv, and then conducted detailed histopathological and ultrastructural studies of khv-infected cells including an examination of the morphology and morphogenesis of khv. the primary target of khv was respiratory epithelial cells of the gill lamellae, and release of virions from infected epithelial cells resulted in a systemic infecti ... | 2008 | 18714678 |
| cloning, nucleotide sequence and phylogenetic analyses, and tissue-specific expression of the transferrin gene in cirrhinus mrigala infected with aeromonas hydrophila. | transferrin partial complementary dnas were cloned from the livers of five species in four genera of indian carps (indian major carp species: labeo rohita, catla catla and cirrhinus mrigala; medium carp: puntius sarana; minor carp: labeo bata) subsequent to polymerase chain reaction amplification with published heterologous primers or self-designed primers derived from conserved regions of transferrin cdna sequences. the partial transferrin cdnas of the five species of carps had sizes from 624 t ... | 2009 | 18718662 |
| influence of food ration, copper exposure and exercise on the energy metabolism of common carp (cyprinus carpio). | the present study was conducted to extend the understanding of the combined physiological effects of different food rations in combination with sublethal levels of copper in common carp (cyprinus carpio). fish acclimated to low (0.5% body weight) and high (5% body weight) food rations were exposed to 1 microm copper for a period of 28 days and kept for a further 14 days in copper free water to examine their recovery. measurements of oxygen consumption, ammonia excretion and ammonia accumulation ... | 2009 | 18725322 |
| differential effects of nitric oxide on rod and cone pathways in carp retina. | the effects of nitric oxide (no) on electroretinograms and light responses of horizontal cells intracellularly recorded from isolated, superfused carp retinas were studied. sodium nitroprusside (snp), an no donor, suppressed scotopic b wave, while enhancing photopic b wave, and the effects could be blocked by hemoglobin, an no chelator. furthermore, following snp application, light responses of rod horizontal cells were reduced in size and those of cone horizontal cells were increased. these res ... | 1997 | 18726301 |
| selective suppression of rod signal transmission by cobalt ions of low levels in carp retina. | selective suppression of rod signal transmission by cobalt ions was reported in carp retina. using 10 munol/l co(2+), rod-driven horizontal cells were hyperpolarized and light responses were completely suppressed in superfused, isolated retina, while cone-driven horizontal cells were almost unaffected. similarly, scotopic electroretinographic bwave was suppressed by 10 munol/l co(2+), while the photopic b-wave remained unaffected. furthermore, the glutamate-isolated receptor potential (piii) was ... | 1997 | 18726308 |
| the onset of foreign gene transcription in nuclear-transferred embryos of fish. | the transcriptional onset ofhgh-transgene in fish was studied in the following three cases: the first is inmthgh-transgenic f(4) common carp (cyprinus carpio) embryos, the second is in nuclear-transferred embryos supported by the transgenic f(4) embryonic nuclei, and the third is in nuclear-transferred embryos supported by the transgenic f(4) tail-fin nuclei. rt-pcr results show that thehgh-transgene initiates its transcriptional activity from early-gastrula stage, the early blastula stage and e ... | 2000 | 18726354 |
| differential expression and characterization analysis of a new gene with wd domains in fish oogenesis. | a new gene with wd domains is cloned and characterized according to its differential transcription and expression between previtellogenic oocytes (phase i oocytes) and fully-grown oocytes (phase v oocytes) from natural gynogenetic silver crucian carp (carassius auratus gibelio) by using the combinative methods of suppressive subtraction hybridization, smart cdna synthesis and race-pcr. the full-length cdna is 1870 bp. its 5' untranslated region is 210 bp, followed by an open reading frame of 990 ... | 2001 | 18726400 |
| differential modulation by ampa of signals from red- and green-sensitive cones in carp retinal luminosity-type horizontal cells. | intracellular recordings were made from luminosity-type horizontal cells (lhcs) in the isolated superfused carp retina and the effect of ampa (alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid), a glutamate receptor agonist, on these cells was studied. ampa suppressed the responses of lhcs driven by red-sensitive (r-) cones whereas it potentiated the responses driven by green-sensitive (g-) cones. the ampa effect could be completely blocked by gyki 53655, a specific ampa receptor antagoni ... | 2001 | 18726418 |
| effect of starvation on transcriptomes of brain and liver in adult female zebrafish (danio rerio). | we used microarray and quantitative real-time pcr (qrt-pcr) analyses in adult female zebrafish (danio rerio) to identify metabolic pathways regulated by starvation in the liver and brain. the transcriptome of whole zebrafish brain showed little response to 21 days of starvation. only agouti-related protein 1 (agrp1) significantly responded, with increased expression in brains of starved fish. in contrast, a 21-day period of starvation significantly downregulated 466 and upregulated 108 transcrip ... | 2008 | 18728227 |
| toxic effects of streptomyces griseus spores and exudate on gill pathology of freshwater fish. | many unexplained fish-kills in british waters are considered microbial in origin and a large proportion of field sites contains elevated concentrations of filamentous actinobacteria. the present study has shown that a strain of streptomyces griseus, isolated from field sites, elicits pathological changes to the gills of fish under laboratory conditions which mirror those found in situ. these changes include hyperplasia leading to fusion of the secondary lamellae and loss of microridging on the f ... | 2009 | 18755510 |
| two unisexual artificial polyploid clones constructed by genome addition of common carp (cyprinus carp) and crucian carp (carassius auratus). | a polyploid hybrid fish with natural gynogenesis can prevent segregation and maintain their hybrid vigor in their progenies. supposing the reproduction mode of induced polyploid fish being natural gynogenesis, allopolyploid hybrid between common carp and crucian carp into allopolyploid was performed. the purpose of this paper is to describe a lineage from sexual diploid carp transforming into allotriploid and allotetraploid unisexual clones by genome addition. the diploid hybrid between common c ... | 2003 | 18758716 |