Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides gd3, gt3, gm2, gd2, gt2, gm1, and gd1a. | we have synthesized several ganglio-oligosaccharide structures using glycosyltransferases from campylobacter jejuni. the enzymes, alpha-(2-->3/8)-sialyltransferase (cst-ii), beta-(1-->4)-n-acetylgalactosaminyltransferase (cgta), and beta-(1-->3)-galactosyltransferase (cgtb), were produced in large-scale fermentation from escherichia coli and further characterized based on their acceptor specificities. 2-azidoethyl-glycosides corresponding to the oligosaccharides of gd3 (alpha-d-neup5ac-(2-->8)-a ... | 2005 | 16005859 |
| the effect of different media on the survival and induction of stress responses by campylobacter jejuni. | the survival kinetics of campylobacter jejuni strain ci 120 to a challenge of ph 4.5 was studied in seven different media. a medium effect was observed, showing up to a 5-log difference in stress resistance of cells. strain variation in survival of c. jejuni was observed in brucella broth (bbl). the ability of c. jejuni ci 120 to respond to a stress after growth in seven different media was also examined. an adaptive tolerance response (atr) was induced in only three of the seven media tested. t ... | 2005 | 16009274 |
| advantages of peptide nucleic acid oligonucleotides for sensitive site directed 16s rrna fluorescence in situ hybridization (fish) detection of campylobacter jejuni, campylobacter coli and campylobacter lari. | traditionally fluorescence in situ hybridization (fish) has been performed with labeled dna oligonucleotide probes. here we present for the first time a high affinity peptide nucleic acid (pna) oligonucleotide sequence for detecting thermotolerant campylobacter spp. using fish. thermotolerant campylobacter spp, including the species campylobacter coli, campylobacter jejuni and campylobacter lari, are important food and water borne pathogens. the designed pna probe (cje195) bound with higher affi ... | 2005 | 16009278 |
| rapid decay of salmonella flagella antibodies during human gastroenteritis: a follow up study. | an indirect enzyme-linked immunosorbent assay (elisa) based on salmonella re-polymerized flagella was employed to measure levels of immunoglobulin (ig) g, igm and iga antibodies in sera from 303 danish patients diagnosed with either salmonella enteritidis or salmonella typhimurium. the antibody-levels were assessed at one, three and six months after onset of salmonellosis, and sera from a control-group of 170 healthy blood donors were additionally analysed in order to establish cut-off values fo ... | 2005 | 16009280 |
| spatial epidemiology and natural population structure of campylobacter jejuni colonizing a farmland ecosystem. | recent progress in determining the population structure of campylobacter jejuni, and discerning associations between genotypes and specific niches, has emphasized the shortfall in our understanding of the ecology and epidemiology of this bacterium. we examined the natural structure of the c. jejuni community associated with cattle farmland in the uk by structured spatiotemporal sampling of habitats, including livestock and wild animal faeces, environmental water and soil, over a 10-week period w ... | 2005 | 16011749 |
| going against the grain: chemotaxis and infection in vibrio cholerae. | chemotaxis is the process by which motile cells move in a biased manner both towards favourable and away from unfavourable environments. the requirement of this process for infection has been examined in several bacterial pathogens, including vibrio cholerae. the single polar flagellum of vibrio species is powered by a sodium-motive force across the inner membrane, and can rotate to produce speeds of up to 60 cell-body lengths (approximately 60microm) per second. investigating the role of the ch ... | 2005 | 16012515 |
| campylobacter surface-layers (s-layers) and immune evasion. | many pathogenic bacteria have evolved mechanisms for evading host immune systems. one evasion mechanism is manifest by the surface layer (s-layer), a paracrystalline protein structure composed of s-layer proteins (slps). the s-layer, possessed by 2 campylobacter species (c. fetus and c. rectus), is external to the bacterial outer membrane and can have multiple functions in immune avoidance. c. fetus is a pathogen of ungulates and immunocompromised humans, in whom it causes disseminated bloodstre ... | 2002 | 16013216 |
| food safety knowledge of consumers and the microbiological and temperature status of their refrigerators. | the objectives of this study were to examine domestic food safety knowledge levels of consumers, establish the levels and incidence of bacterial contamination and operational temperatures in domestic refrigerators, and identify areas in which consumer food safety education is necessary in ireland. a food safety knowledge questionnaire applied to a representative sample of households (n = 1,020) throughout the island of ireland found the gaps in consumer food safety knowledge. analysis of swab sa ... | 2005 | 16013380 |
| paenibacillus polymyxa purified bacteriocin to control campylobacter jejuni in chickens. | campylobacter spp. cause numerous foodborne diseases. poultry is thought to be a significant source of this zoonosis. although many interventions designed to control this agent have been researched, none have succeeded. we evaluated a bacteriocin-based treatment to reduce campylobacter jejuni colonization in poultry. a previously described purified bacteriocin (class iia; molecular mass, 3,864 da), secreted by paenibacillus polymyxa nrrl-b-30509, was microencapsulated in polyvinylpyrrolidone, an ... | 2005 | 16013385 |
| microbicidal activity of tripotassium phosphate and fatty acids toward spoilage and pathogenic bacteria associated with poultry. | the ability of solutions of tripotassium phosphate (tpp) and fatty acids (lauric and myristic acids) to reduce populations of spoilage and pathogenic microorganisms associated with processed poultry was examined. in vitro studies were conducted with cultures of bacteria (campylobacter jejuni, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, and staphylococcus aureus) and yeasts (candida ernobii and yarrowia lipolytica). cultures of the bacteria and yeasts ... | 2005 | 16013388 |
| [establishment of immunomagnetic capture-fluorescent pcr detection method for campylobacter jejuni]. | in order to develop a rapid method which can check campylobacter jejuni in animal and poultry foods nicely, an immunomagnetic capture-fluorescent pcr (imc-fpcr) method was established in this paper. the reported method involves isolation of the target pathogen by immunocapture prior to the fluorescent pcr step, therefore the immunomagnetic-beads for campylobacter were developed, and two groups of primer/probe, which targeted for the species special sequence of flaa gene and hipo gene for campylo ... | 2005 | 16013502 |
| improved serodiagnosis of campylobacter jejuni infections using recombinant antigens. | campylobacter jejuni is a frequent cause of infectious diarrhoea and is increasingly recognized as a trigger for late-onset complications. the poor standardization of commonly used serological tests might explain the conflicting results regarding the frequency of antecedent c. jejuni infections in defined patient groups. in order to obtain reliable epidemiological data as to the role of c. jejuni in causing late-onset complications, a highly specific and sensitive diagnostic tool for the epidemi ... | 2005 | 16014430 |
| differential effect of the cytolethal distending toxin of actinobacillus actinomycetemcomitans on co-cultures of human oral cells. | the periodontal pathogen actinobacillus actinomycetemcomitans expresses a cytolethal distending toxin (cdt) that typically arrests the growth of eukaryotic cells at either the g0/g1 or g2/m phase of the cell cycle. it was previously found that cdt failed to arrest the growth of human periodontal ligament fibroblasts (hplfs) when grown in pure culture. in contrast, proliferation of an oral epithelial cell line was rapidly inhibited by the toxin. in this study, the feasibility of using mixed-cell ... | 2005 | 16014433 |
| resistance of human periodontal ligament fibroblasts to the cytolethal distending toxin of actinobacillus actinomycetemcomitans. | the cytolethal distending toxin (cdt) of actinobacillus actinomycetemcomitans is a typical member of this gram-negative bacterium holotoxin family that targets a wide spectrum of eukarytotic cells, typically causing cell cycle arrest at either the g(1) or g(2)/m phase of the cell cycle. in view of the possible role of the cdt as a prominent a. actinomycetemcomitans virulence factor in periodontal diseases, we have examined the effects of the toxin on primary cultures of human periodontal ligamen ... | 2005 | 16018764 |
| enterotoxigenic escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention. | etec is an underrecognized but extremely important cause of diarrhea in the developing world where there is inadequate clean water and poor sanitation. it is the most frequent bacterial cause of diarrhea in children and adults living in these areas and also the most common cause of traveler's diarrhea. etec diarrhea is most frequently seen in children, suggesting that a protective immune response occurs with age. the pathogenesis of etec-induced diarrhea is similar to that of cholera and include ... | 2005 | 16020685 |
| epidemiology and management of infectious diseases in international adoptees. | international adoptees represent a group of children with unique health care needs. data from published studies, along with the recent experience of the yale international adoption clinic, suggest that the risk of serious infections in adoptees is low, although infections associated with institutionalization still occur commonly. interpretation of these data must be undertaken with caution, however, since many, if not most, international adoptees are not evaluated in specialty clinics. thus, pro ... | 2005 | 16020687 |
| phage therapy reduces campylobacter jejuni colonization in broilers. | the effect of phage therapy in the control of campylobacter jejuni colonization in young broilers, either as a preventive or a therapeutic measure, was tested. a prevention group was infected with c. jejuni at day 4 of a 10-day phage treatment. a therapeutic group was phage treated for 6 days, starting 5 days after c. jejuni colonization of the broilers had been established. treatment was monitored by enumerating campylobacter colony forming units (cfu) and phage plaque forming units (pfu) from ... | 2005 | 16024187 |
| [viral gastroenteritis in children]. | purpose of the trial: to establish the involvement of viruses in the aetiology of diarrhoeal disorders in children, the incidence of individual viruses in various age groups and in different seasons, the impact of the aetiological agent on the clinical picture, the severity of the disorder, therapy and duration of hospital stay. the significance of intestinal viruses as nosocomial pathogens,a comparison of the sensitivity of latex agglutination and electron microscopy in the diagnosis of rotavir ... | 2005 | 16025426 |
| identification of a novel gene encoding a flavin-dependent trna:m5u methyltransferase in bacteria--evolutionary implications. | formation of 5-methyluridine (ribothymidine) at position 54 of the t-psi loop of trna is catalyzed by site-specific trna methyltransferases (trna:m(5)u-54 mtase). in all eukarya and many gram-negative bacteria, the methyl donor for this reaction is s-adenosyl-l-methionine (s-adomet), while in several gram-positive bacteria, the source of carbon is n(5), n(10)-methylenetetrahydrofolate (ch(2)h(4)folate). we have identified the gene for bacillus subtilis trna:m(5)u-54 mtase. the encoded recombinan ... | 2005 | 16027442 |
| campylobacter jejuni pancolitis mimicking idiopathic ulcerative colitis. | campylobacter jejuni is the most common cause of community-acquired acute bacterial diarrhea. campylobacter diarrhea is usually accompanied by fever and abdominal pain. campylobacter diarrhea is usually watery. nausea, vomiting, headache, and myalgias may also be present. tenesmus is a common feature. the majority of patients with campylobacter diarrhea have some component of segmental colitis, usually beginning in the small bowel and progressing distally to the cecum and colon. c. jejuni is a r ... | 2005 | 16027651 |
| identification of a d-glycero-d-manno-heptosyltransferase gene from helicobacter pylori. | we have identified a helicobacter pylori d-glycero-d-manno-heptosyltransferase gene, hp0479, which is involved in the biosynthesis of the outer core region of h. pylori lipopolysaccharide (lps). insertional inactivation of hp0479 resulted in formation of a truncated lps molecule lacking an alpha-1,6-glucan-, dd-heptose-containing outer core region and o-chain polysaccharide. detailed structural analysis of purified lps from hp0479 mutants of strains ss1, 26695, o:3, and pj1 by a combination of c ... | 2005 | 16030209 |
| seasonal prevalence of thermophilic campylobacter infections in dairy cattle and a study of infection of sheep. | a total of 273 rectal swabs from dairy cows were cultured for campylobacter jejuni/coli. the isolation rate was 17/72 (24%), 33/106 (31%) and 11/95 (12%) during summer, autumn and winter respectively. approximately half of the isolates were c. jejuni and the other half c. coli. the isolates recovered from dairy cows were typed by bacterial restriction endonuclease dna analysis (brenda) and compared with those of sheep. seventeen different brenda patterns were produced by the isolates from dairy ... | 1989 | 16031500 |
| attempted definition by immunoblotting of the causes of reactivity in suspected false-positive sera in the brucella ovis complement fixation test. | seventy-nine suspected false-positive sera, obtained over 1 year from routine submissions for brucella ovis serological testing, were used in this study. these sera, which exhibited titres in the complement fixation test, but which because of their epidemiological history and their reactions in the enzyme-linked immunosorbent assay and gel diffusion test were suspected to be false positives, were further analysed by immunoblotting. in blots, using b. ovis antigens, rough lipopolysaccharide was i ... | 1996 | 16031926 |
| antimicrobial resistance patterns of campylobacter from feedlot cattle*. | this study examined 448 campylobacter strains isolated in 1999 and 2000 from us feedlot cattle for resistance to 12 antimicrobials. | 2005 | 16033459 |
| development of real-time pcr and hybridization methods for detection and identification of thermophilic campylobacter spp. in pig faecal samples. | to develop a real-time (rt) pcr for species differentiation of thermophilic campylobacter and to develop a method for assessing co-colonization of pigs by campylobacter spp. | 2005 | 16033460 |
| genotyping of campylobacter jejuni strains from danish broiler chickens by restriction fragment length polymorphism of the lps gene cluster. | to apply and evaluate lg (lps genes) genotyping, which is a genotyping method based on a cluster of genes involved in the synthesis of surface lipopolysaccharides (lps) in campylobacter species, for typing of campylobacter jejuni isolates obtained from danish broiler chickens. furthermore, the lg genotyping method was used to study the genetic stability of four c. jejuni strains after gastrointestinal passage through experimentally infected chickens. | 2005 | 16033471 |
| promoter analysis of cytolethal distending toxin genes (cdta , b, and c ) and effect of a luxs mutation on cdt production in campylobacter jejuni. | quorum sensing is known to be related to regulation of virulence factors in some pathogens. we report transcriptional analysis of cdt genes (cdta, b, and c ) encoding cytolethal distending toxin (cdt) in campylobacter jejuni and demonstrate that a luxs mutation affects the cdt transcription in c. jejuni. the reverse transcriptase-polymerase chain reaction (rt-pcr) showed that cdta, cdtb, and cdtc genes constitute a polycistronic operon in c. jejuni. a putative transcriptional start site was foun ... | 2005 | 16034202 |
| protein-protein interaction of hp137 with histidine kinase hp244 does not contribute to flagellar regulation in helicobacter pylori. | flagellar motility is essential for the ability of helicobacter pylori to colonize the gastric mucosa. expression of the flagella is controlled by a complex regulatory cascade involving the two-component system flgr-hp244, the sigma factors sigma54 and sigma28 and the anti-sigma28 factor flgm. the protein-protein interaction map of h. pylori, which is based on a high-throughput two-hybrid screen (rain et al., 2001. nature 409, 211-215) indicated a protein-protein interaction between the gene pro ... | 2005 | 16035242 |
| effect of antibiotic sub inhibitory concentration on cytolethal distending toxin production by campylobacter jejuni. | to investigate the effect of pre-exposure antibiotics on cytolethal distending toxin (cdt) production and toxigenic effect of c. jejuni. | 2004 | 16038766 |
| involvement of ganglioside gm3 in g(2)/m cell cycle arrest of human monocytic cells induced by actinobacillus actinomycetemcomitans cytolethal distending toxin. | actinobacillus actinomycetemcomitans produces a toxin called cytolethal distending toxin (cdt), which causes host cell dna damage leading to the induction of dna damage checkpoint pathways. cdt consists of three subunits, cdta, cdtb, and cdtc. cdtb is the active subunit of cdt and exerts its effect as a nuclease that damages nuclear dna, triggering cell cycle arrest. in the present study, we confirmed that the only combination of toxin proteins causing cell cycle arrest was that of all three rec ... | 2005 | 16040998 |
| expression of arg-gingipain rgpb is required for correct glycosylation and stability of monomeric arg-gingipain rgpa from porphyromonas gingivalis w50. | arg-gingipains are extracellular cysteine proteases produced by the gram-negative periodontal pathogen porphyromonas gingivalis and are encoded by rgpa and rgpb. three arg-gingipains, heterodimeric high-molecular-mass arg-gingipain hrgpa comprising the alpha-catalytic chain and the beta-adhesin chain, the monomeric soluble arg-gingipain comprising only the alpha-catalytic chain (rgpa(cat)), and the monomeric membrane-type heavily glycosylated arg-gingipain comprising the alpha-catalytic chain (m ... | 2005 | 16041000 |
| intracellular survival of campylobacter jejuni in human monocytic cells and induction of apoptotic death by cytholethal distending toxin. | campylobacter jejuni 81-176 is capable of extensive replication within human monocytic cell vacuoles and induces apoptotic death via cytolethal distending toxin. | 2005 | 16041038 |
| campylobacter jejuni gene expression in the chick cecum: evidence for adaptation to a low-oxygen environment. | transcriptional profiling of campylobacter jejuni during colonization of the chick cecum identified 59 genes that were differentially expressed in vivo compared with the genes in vitro. the data suggest that c. jejuni regulates electron transport and central metabolic pathways to alter its physiological state during establishment in the chick cecum. | 2005 | 16041056 |
| active membrane transport and receptor proteins from bacteria. | a general strategy for the expression of bacterial membrane transport and receptor genes in escherichia coli is described. expression is amplified so that the encoded proteins comprise 5-35% of e. coli inner membrane protein. depending upon their topology, proteins are produced with rgsh6 or a strep tag at the c-terminus. these enable purification in mg quantities for crystallization and nmr studies. examples of one nutrient uptake and one multidrug extrusion protein from helicobacter pylori are ... | 2005 | 16042616 |
| analysis of recombination in campylobacter jejuni from mlst population data. | we analyze recombination in c. jejuni using mlst data from isolates taken from wild birds, cattle, wild rabbits, and water in a 100-km2 study region in cheshire, uk. we use a recent approximate likelihood method for inference, based on combining likelihood information from all pairs of segregating (polymorphic) sites in the data. we find substantial evidence for recombination, but only for recombination with short tract lengths, of around 225-750 bp. we estimate that the rate of recombination is ... | 2005 | 16044246 |
| [campylobacter jejuni bacteriemia associated with acute pancreatitis]. | 2005 | 16044618 | |
| nssr, a member of the crp-fnr superfamily from campylobacter jejuni, regulates a nitrosative stress-responsive regulon that includes both a single-domain and a truncated haemoglobin. | consistent with its role as a nitric oxide (no)-detoxifying globin in campylobacter jejuni, cgb (campylobacter globin) expression is strongly and specifically induced following exposure to nitrosative stress, suggesting a previously unrecognized capacity for no-related stress sensing in this food-borne pathogen. in this study, fur and perr have been eliminated as major regulators of cgb, and nssr (cj0466), a member of the crp-fnr superfamily, has been identified as the major positive regulatory ... | 2005 | 16045618 |
| bacteriological evaluation of commercial canine and feline raw diets. | twenty-five commercial raw diets for dogs and cats were evaluated bacteriologically. coliforms were present in all diets, ranging from 3.5 x 10(3) to 9.4 x 10(6) cfu/g (mean 8.9 x 10(5); standard deviation 1.9 x 10(6)). escherichia coli was identified in 15/25 (64%) diets; however, e. coli o157 was not detected. salmonella spp. were detected in 5/25 (20%) diets; 1 each of beef-, lamb-, quail-, chicken-, and ostrich-based diets. sporeforming bacteria were identified from 4/25 (16%) samples on dir ... | 2005 | 16048011 |
| real-time pcr screening for 16s rrna mutations associated with resistance to tetracycline in helicobacter pylori. | the effectiveness of recommended first-line therapies for helicobacter pylori infections is decreasing due to the occurrence of resistance to metronidazole and/or clarithromycin. quadruple therapies, which include tetracycline and a bismuth salt, are useful alternative regimens. however, resistance to tetracycline, mainly caused by mutations in the 16s rrna genes (rrna and rrnb) affecting nucleotides 926 to 928, are already emerging and can impair the efficacies of such second-line regimens. her ... | 2005 | 16048919 |
| role of efflux pumps and topoisomerase mutations in fluoroquinolone resistance in campylobacter jejuni and campylobacter coli. | point mutations in the topoisomerase (dna gyrase a) gene are known to be associated with fluoroquinolone resistance in campylobacter. recent studies have shown that an efflux pump encoded by cmeabc is also involved in decreased susceptibilities to fluoroquinolones, as well as other antimicrobials. genome analysis suggests that campylobacter jejuni contains at least nine other putative efflux pumps. using insertional inactivation and site-directed mutagenesis, we investigated the potential contri ... | 2005 | 16048946 |
| a reconsideration of the campylobacter dose-response relation. | as a major foodborne pathogen, campylobacter jejuni receives much attention in quantitative risk assessment. to date, all dose-response assessments have been based on a single human feeding study which unfortunately provides incomplete and possibly biased information on the dose-response relation. an incident at a dairy farm, where several children from a school class became ill as a result of drinking raw milk contaminated with c. jejuni, appeared to show a very clear dose-response relation bet ... | 2005 | 16050502 |
| a large outbreak of campylobacteriosis associated with a municipal water supply in finland. | in august 1998, an outbreak of campylobacteriosis occurred in one municipality in northern finland. a 10% random sample of residents (population 15 000) was selected through the national population registry for a survey conducted by using postal questionnaires. cases were defined as residents of the municipality with onset of acute gastroenteritis from 1 to 20 august 1998. of 1167 respondents (response rate 78%), 218 (18.7%) met the case definition. drinking non-chlorinated municipal tap water w ... | 2005 | 16050503 |
| brucella melitensis infection associated with guillain-barré syndrome through molecular mimicry of host structures. | brucella melitensis is a facultative intracellular bacterium that can survive inside macrophages and the causative agent of brucellosis. in the present study, we found that a lipooligosaccharide of b. melitensis has a gm1 ganglioside-like structure and shows a strong antibody response in mice. the cholera toxin b subunit, which binds to gm1 ganglioside specifically, reacted with the surface of b. melitensis. immunization with b. melitensis induced the production of anti-gm1 ganglioside antibodie ... | 2005 | 16051063 |
| genomic and proteomic identification of a dna-binding protein used in the "fingerprinting" of campylobacter species and strains by maldi-tof-ms protein biomarker analysis. | we have identified a prominent approximately 10-kda protein biomarker observed in the matrix-assisted laser desorption/ionization time-of-flight mass spectra (maldi-tof-ms) of cell lysates of five thermophilic species of campylobacter: jejuni, coli, lari, upsaliensis, and helveticus. the biomarker was unambiguously identified by genomic and proteomic sequencing as a dna-binding protein hu. we report the amino acid sequence of hu as determined by sequencing the hup gene of four species (12 strain ... | 2005 | 16053303 |
| cell-surface alpha-glucan in campylobacter jejuni 81-176. | campylobacter jejuni infection is a main source of severe gastroenteritis-related illnesses in humans and there is also evidence that it may be linked to neurological disorders. c. jejuni 81-176 is a virulent strain that has become the global model in the study of mechanisms and pathogenesis of c. jejuni infection. for this reason, we were engaged in studying the fine structures of cell-surface carbohydrate antigens of c. jejuni 81-176, namely, the capsule polysaccharide (cps) and lipooligosacch ... | 2005 | 16055105 |
| development and application of oligonucleotide probes for in situ detection of thermotolerant campylobacter in chicken faecal and liver samples. | based on campylobacter 16s- and 23s-rrna sequence data oligonucleotide probes specific for thermotolerant campylobacters and for members of the genus campylobacter have been developed. the 16s-rrna-targeted probe camp 653, recommended for a comprehensive detection of members of the genus campylobacter, specifically detected all campylobacter strains used in this study. detection of thermotolerant species has been achieved by the 23s-rrna-targeted probe cajeco1427. optimal hybridisation condition ... | 2005 | 16061298 |
| detection of pathogenic bacteria in food samples using highly-dispersed carbon particles. | there is an unmet need for detection methods that can rapidly and sensitively detect food borne pathogens. a flow through immunoassay system utilizing highly dispersed carbon particles and an amperometric technique has been developed and optimized. a sandwich immunoassay format was utilized in which pathogenic cells were captured by antibodies immobilized onto activated carbon particles, and labeled with horseradish peroxidase (hrp) conjugated antibodies. flow of the peroxidase substrates result ... | 2005 | 16076439 |
| spatial location and requirements for the assembly of the agrobacterium tumefaciens type iv secretion apparatus. | type iv secretion is used by pathogenic microorganisms to transfer effector macromolecules to eukaryotic target cells. the virb/d4 apparatus of agrobacterium tumefaciens transfers dna and proteins to plant cells. we postulated that the cell pole is the site of assembly of the a. tumefaciens type iv apparatus. using immunofluorescence microscopy, we now demonstrate that 10 of the virb proteins localized primarily to one cell pole and a macromolecular virb complex is assembled at the pole. neither ... | 2005 | 16076948 |
| crystal structure of the bacterial yhch protein indicates a role in sialic acid catabolism. | the yhch gene is part of the nan operon in bacteria that encodes proteins involved in sialic acid catabolism. determination of the crystal structure of yhch from haemophilus influenzae was undertaken as part of a structural genomics effort in order to assist with the functional assignment of the protein. the structure was determined at 2.2-a resolution by multiple-wavelength anomalous diffraction. the protein fold is a variation of the double-stranded beta-helix. two antiparallel beta-sheets for ... | 2005 | 16077096 |
| phylogenetic analysis of pasteuria penetrans by use of multiple genetic loci. | pasteuria penetrans is a gram-positive, endospore-forming eubacterium that apparently is a member of the bacillus-clostridium clade. it is an obligate parasite of root knot nematodes (meloidogyne spp.) and preferentially grows on the developing ovaries, inhibiting reproduction. root knot nematodes are devastating root pests of economically important crop plants and are difficult to control. consequently, p. penetrans has long been recognized as a potential biocontrol agent for root knot nematode ... | 2005 | 16077116 |
| helicobacter pylori flagellar hook-filament transition is controlled by a flik functional homolog encoded by the gene hp0906. | helicobacter pylori is a human gastric pathogen which is dependent on motility for infection. the h. pylori genome encodes a near-complete complement of flagellar proteins compared to model enteric bacteria. one of the few flagellar genes not annotated in h. pylori is that encoding flik, a hook length control protein whose absence leads to a polyhook phenotype in salmonella enterica. we investigated the role of the h. pylori gene hp0906 in flagellar biogenesis because of linkage to other flagell ... | 2005 | 16077121 |
| campylobacter jejuni activates mitogen-activated protein kinases in caco-2 cell monolayers and in vitro infected primary human colonic tissue. | the mitogen-activated protein kinases (mapks) play a central role in many host signalling pathways. these signalling proteins are known to be involved in host responses against invasive bacteria including generation of chemotactic and inflammatory cytokines. it was hypothesized that campylobacter jejuni may activate mapks, as intestinal infection may induce a clinical and pathological picture of acute colonic inflammation. infection of caco-2 cell monolayers (human colonic epithelial cell line) ... | 2005 | 16079352 |
| dna sequence-based subtyping and evolutionary analysis of selected salmonella enterica serotypes. | while serotyping and phage typing have been used widely to characterize salmonella isolates, sensitive subtyping methods that allow for evolutionary analyses are essential for examining salmonella transmission, ecology, and evolution. a set of 25 salmonella enterica isolates, representing five clinically relevant serotypes (serotypes agona, heidelberg, schwarzengrund, typhimurium, and typhimurium var. copenhagen) was initially used to develop a multilocus sequence typing (mlst) scheme for salmon ... | 2005 | 16081897 |
| use of the roche lightcycler strep b assay for detection of group b streptococcus from vaginal and rectal swabs. | the results for a real-time pcr assay, using the lightcycler strep b analyte-specific reagents (roche diagnostics corporation, indianapolis, ind.), were compared to a direct plate method combined with a broth enrichment culture method for detection of group b streptococcus colonization in pregnant women. two separate evaluations were conducted using two different automated nucleic extraction instruments, the magna pure lc instrument (roche diagnostics corporation) and the lower-capacity magna pu ... | 2005 | 16081949 |
| genetic diversity of campylobacter sp. isolates from retail chicken products and humans with gastroenteritis in central michigan. | multilocus sequencing was used to compare campylobacter sp. strains isolated from retail chicken products and humans with gastroenteritis in central michigan. sequence comparisons demonstrated overlapping diversity between chicken and human isolates. campylobacter jejuni isolates from clinical sources had a greater diversity of flagellin alleles and a higher rate of quinolone resistance than isolates from retail chicken products. | 2005 | 16081984 |
| risk factors for contamination of ready-to-eat street-vended poultry dishes in dakar, senegal. | our objective was to investigate the salmonella and campylobacter contamination of traditional ready-to-eat street-vended poultry dishes and to assess the association of some restaurant characteristics and cooking practices with the contamination of these meals. one hundred and forty-eight street-restaurants were studied from january 2003 to april 2004 in dakar. a questionnaire was submitted to the managers, and samples of ready-to-eat poultry dishes were taken. salmonella spp. was isolated in 2 ... | 2005 | 16083818 |
| [prevalence and characteristics of guillain-barré syndromes associated with campylobacter jejuni and cytomegalovirus in greater paris]. | we aimed to study prevalence and features of campylobacter jejuni and cytomegalovirus (cmv)-associated guillain-barré syndromes (gbs) in a french care unit. | 2005 | 16084033 |
| inhibition of clostridium perfringens by a novel strain of bacillus subtilis isolated from the gastrointestinal tracts of healthy chickens. | the objectives of this study were to isolate beneficial strains of microorganisms from the gastrointestinal tracts of healthy chickens and to screen them against clostridium perfringens, a causative agent of necrotic enteritis in poultry. one of the bacteria isolated, a strain of bacillus subtilis, was found to possess an anticlostridial factor that could inhibit the c. perfringens atcc 13124 used in this study. the anticlostridial factor produced by b. subtilis pb6 was found to be fully or part ... | 2005 | 16085801 |
| discrimination of enterobacterial repetitive intergenic consensus pcr types of campylobacter coli and campylobacter jejuni by fourier transform infrared spectroscopy. | fourier transform infrared spectroscopy (ft-ir) has been used together with pattern recognition methodology to study isolates belonging to the species campylobacter coli and campylobacter jejuni and to compare ft-ir typing schemes with established genomic profiles based on enterobacterial repetitive intergenic consensus pcr (eric-pcr). seventeen isolates were cultivated under standardized conditions for 2, 3, and 4 days to study variability and improve reproducibility. eric-pcr profiles and ft-i ... | 2005 | 16085819 |
| concurrent quantitation of total campylobacter and total ciprofloxacin-resistant campylobacter loads in rinses from retail raw chicken carcasses from 2001 to 2003 by direct plating at 42 degrees c. | this is the first report on the use of a normally lethal dose of ciprofloxacin in a campylobacter agar medium to kill all ciprofloxacin-sensitive campylobacter spp. but allow the selective isolation and quantitation of naturally occurring presumptive ciprofloxacin-resistant campylobacter cfu in rinses from retail raw chicken carcasses (rtcc). thermophilic-group total campylobacter cfu and total ciprofloxacin-resistant campylobacter cfu (irrespective of species) were concurrently quantified in ri ... | 2005 | 16085843 |
| correlation of campylobacter bacteriophage with reduced presence of hosts in broiler chicken ceca. | campylobacter jejuni and campylobacter-specific bacteriophage were enumerated from broiler chicken ceca selected from 90 united kingdom flocks (n = 205). c. jejuni counts in the presence of bacteriophage (mean log(10) 5.1 cfu/g) were associated with a significant (p < 0.001) reduction compared to samples with campylobacter alone (mean log(10) 6.9 cfu/g). | 2005 | 16085889 |
| identification of a fibronectin-binding domain within the campylobacter jejuni cadf protein. | the binding of campylobacter jejuni to fibronectin (fn), a component of the extracellular matrix, is mediated by a 37 kda outer membrane protein termed cadf for campylobacter adhesion to fn. previous studies have indicated that c. jejuni binds to fn on the basolateral surface of t84 human colonic cells. to further characterize the interaction of the cadf protein with fn, enzyme-linked immunosorbent assays were performed to identify the fn-binding domain (fn-bd). using overlapping 30-mer and 16-m ... | 2005 | 16091041 |
| structural and dynamic properties of bacterial type iv secretion systems (review). | the type iv secretion systems (t4ss) are widely distributed among the gram-negative and -positive bacteria. these systems mediate the transfer of dna and protein substrates across the cell envelope to bacterial or eukaryotic cells generally through a process requiring direct cell-to-cell contact. bacteria have evolved t4ss for survival during establishment of pathogenic or symbiotic relationships with eukaryotic hosts. the agrobacterium tumefaciens virb/d4 t4ss and related conjugation machines s ... | 2005 | 16092524 |
| a newly constructed primer pair for the pcr amplification, cloning and sequencing of the flagellin (flaa) gene from isolatesof urease-negative campylobacter lari. | a newly constructed primer pair (lari-af/lari-ar) designed to generate a product of the flagellin (flaa) gene for urease-negative campylobacter lari produced a pcr amplicon of about 1700 bp for 16 isolates from 7 seagulls, 5 humans, 3 food animals and one mussel in japan and northern ireland. nucleotide sequencing and alignments of the flaa amplicons from these isolates demonstrated that the deduced amino acid sequences of the possible open reading frame were 564-572 amino acid residues in lengt ... | 2005 | 16096687 |
| electrophoresis-assisted open-tubular liquid chromatography/mass spectrometry for the analysis of lipooligosaccharide expressed by campylobacter jejuni. | lipooligosaccharide (los) is the major component of the external membrane of campylobacter jejuni. los contains a hydrophobic moiety, lipid a, and a hydrophilic moiety, oligosaccharide. due to the unique mimicry of human ganglioside structures and potential involvement in the induction of the autoimmune polyneuropathies, guillain-barré and miller fisher syndromes, the structural characterization of c. jejuni los has received much attention. we have been using capillary zone electrophoresis-mass ... | 2005 | 16097024 |
| establishment of a non-human primate campylobacter disease model for the pre-clinical evaluation of campylobacter vaccine formulations. | campylobacter jejuni is a common cause of enteritis worldwide. the mechanisms by which c. jejuni causes disease are unclear. challenge studies in humans are currently considered unethical due to the possibility of severe complications, such as guillain-barré syndrome. campylobacter infection in non-human primates closely mimics the disease and immune response, seen in humans. in this study, we attempted to determine the minimal dose of a pathogenic c. jejuni 81-176 strain required for clinical s ... | 2006 | 16098634 |
| absence of the plasmid-mediated quinolone resistance qnra gene among campylobacter jejuni clinical isolates from greece. | 2005 | 16099626 | |
| campylobacter spp., salmonella spp., verocytotoxic escherichia coli, and antibiotic resistance in indicator organisms in wild cervids. | faecal samples were collected, as part of the national health surveillance program for cervids (hop) in norway, from wild red deer, roe deer, moose and reindeer during ordinary hunting seasons from 2001 to 2003. samples from a total of 618 animals were examined for verocytotoxic e. coli (vtec); 611 animals for salmonella and 324 animals for campylobacter. a total of 50 samples were cultivated from each cervid species in order to isolate the indicator bacterial species e. coli and enterococcus fa ... | 2005 | 16108210 |
| a phylogeny of bacterial rna nucleotidyltransferases: bacillus halodurans contains two trna nucleotidyltransferases. | we have analyzed the distribution of rna nucleotidyltransferases from the family that includes poly(a) polymerases (pap) and trna nucleotidyltransferases (tnt) in 43 bacterial species. genes of several bacterial species encode only one member of the nucleotidyltransferase superfamily (ntsf), and if that protein functions as a tnt, those organisms may not contain a poly(a) polymerase i like that of escherichia coli. the genomes of several of the species examined encode more than one member of the ... | 2005 | 16109934 |
| heat shock response of archaeoglobus fulgidus. | the heat shock response of the hyperthermophilic archaeon archaeoglobus fulgidus strain vc-16 was studied using whole-genome microarrays. on the basis of the resulting expression profiles, approximately 350 of the 2,410 open reading frames (orfs) (ca. 14%) exhibited increased or decreased transcript abundance. these span a range of cell functions, including energy production, amino acid metabolism, and signal transduction, where the majority are uncharacterized. one orf called af1298 was identif ... | 2005 | 16109946 |
| discordant 16s and 23s rrna gene phylogenies for the genus helicobacter: implications for phylogenetic inference and systematics. | analysis of 16s rrna gene sequences has become the primary method for determining prokaryotic phylogeny. phylogeny is currently the basis for prokaryotic systematics. therefore, the validity of 16s rrna gene-based phylogenetic analyses is of fundamental importance for prokaryotic systematics. discrepancies between 16s rrna gene analyses and dna-dna hybridization and phenotypic analyses have been noted in the genus helicobacter. to clarify these discrepancies, we sequenced the 23s rrna genes for ... | 2005 | 16109952 |
| xanthomonas campestris pv. campestris possesses a single gluconeogenic pathway that is required for virulence. | disruption of ppsa, a key gene in gluconeogenesis, of xanthomonas campestris pv. campestris resulted in the failure of the pathogen to grow in medium with pyruvate or c4-dicarboxylates as the sole carbon source and a significant reduction in virulence, indicating that x. campestris pv. campestris possesses only the malic enzyme-ppsa route in gluconeogenesis, which is required for virulence. | 2005 | 16109965 |
| real-time polymerase chain reaction to diagnose lymphogranuloma venereum. | 2005 | 16110579 | |
| identification of a new family of enzymes with potential o-acetylpeptidoglycan esterase activity in both gram-positive and gram-negative bacteria. | the metabolism of the rigid bacterial cell wall heteropolymer peptidoglycan is a dynamic process requiring continuous biosynthesis and maintenance involving the coordination of both lytic and synthetic enzymes. the o-acetylation of peptidoglycan has been proposed to provide one level of control on these activities as this modification inhibits the action of the major endogenous lytic enzymes, the lytic transglycosylases. the o-acetylation of peptidoglycan also inhibits the activity of the lysozy ... | 2005 | 16111493 |
| concurrent infection with an intestinal helminth parasite impairs host resistance to enteric citrobacter rodentium and enhances citrobacter-induced colitis in mice. | infections with intestinal helminth and bacterial pathogens, such as enteropathogenic escherichia coli, continue to be a major global health threat for children. to test the hypothesis that intestinal helminth infection may be a risk factor for enteric bacterial infection, a murine model was established by using the intestinal helminth heligomosomoides polygyrus. to analyze the modulatory effect of a th2-inducing helminth on the outcome of enteric bacterium citrobacter rodentium infection, balb/ ... | 2005 | 16113263 |
| role of flif and flii of listeria monocytogenes in flagellar assembly and pathogenicity. | flagellar structures have been shown to participate in virulence in a variety of intestinal pathogens. here, we have identified two potential flagellar genes of listeria monocytogenes: lmo0713, encoding a protein similar to the flagellar basal body component flif, and lmo0716, encoding a protein similar to flii, the cognate atpase energizing the flagellar export apparatus. expression of flif and flii appears to be downregulated at 37 degrees c, like that of flaa, encoding flagellin. by construct ... | 2005 | 16113269 |
| bacterial contamination of vhuswa--a local weaning food and stored drinking-water in impoverished households in the venda region of south africa. | bacterial contaminants of vhuswa--a traditional maize-based weaning food, and domestic drinking-water stored in impoverished rural households in venda of limpopo province, south africa, were determined. one hundred and twenty-five samples of vhuswa fed to children aged less than five years were assessed for escherichia coli, campylobacter jejuni, salmonella, and shigella. the microbiological quality of 125 drinking-water samples was also evaluated using total coliforms, faecal coliforms, and fae ... | 2005 | 16117367 |
| does age acquired immunity confer selective protection to common serotypes of campylobacter jejuni? | campylobacter infection is a major cause of bacterial gastrointestinal disease. exposure to campylobacter is known to produce an immune response in humans that can prevent future symptomatic infections. further, studies of the general population have shown that seroprevalence to campylobacter increases with age. | 2005 | 16117832 |
| genomes are covered with ubiquitous 11 bp periodic patterns, the "class a flexible patterns". | the genomes of prokaryotes and lower eukaryotes display a very strong 11 bp periodic bias in the distribution of their nucleotides. this bias is present throughout a given genome, both in coding and non-coding sequences. until now this bias remained of unknown origin. | 2005 | 16120222 |
| identification and characterization of neub3 from campylobacter jejuni as a pseudaminic acid synthase. | campylobacter jejuni and campylobacter coli are the main causes of bacterial diarrhea worldwide, and helicobacter pylori is known to cause duodenal ulcers. in all of these pathogenic organisms, the flagellin proteins are heavily glycosylated with a 2-keto-3-deoxy acid, pseudaminic acid (5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-nonulosonic acid). the presence of pseudaminic acid is required for the proper development of the flagella and is thereby necessary for motility in, and invasi ... | 2005 | 16120604 |
| campylobacter coli: prevalence and antimicrobial resistance in antimicrobial-free (abf) swine production systems. | to determine the prevalence and antimicrobial resistance of campylobacter species in swine reared in the intensive and extensive antimicrobial-free (abf) production systems at farm and slaughter. in the abf system, antimicrobials are neither used for growth promotion nor therapeutic purposes. | 2005 | 16120624 |
| inhibition of bacterial rna polymerase by streptolydigin: stabilization of a straight-bridge-helix active-center conformation. | we define the target, mechanism, and structural basis of inhibition of bacterial rna polymerase (rnap) by the tetramic acid antibiotic streptolydigin (stl). stl binds to a site adjacent to but not overlapping the rnap active center and stabilizes an rnap-active-center conformational state with a straight-bridge helix. the results provide direct support for the proposals that alternative straight-bridge-helix and bent-bridge-helix rnap-active-center conformations exist and that cycling between st ... | 2005 | 16122422 |
| [a case of acute cholecystitis by campylobacter jejuni occuring after campylobacter colitis]. | 2005 | 16124715 | |
| toxic megacolon complicating escherichia coli o157 infection. | toxic megacolon is a well known complication in inflammatory bowel disease such as ulcerative colitis or crohn's disease. the development of toxic megacolon as a complication of infectious colitis is rare. however it is recognised as a complication of enteric infections caused by clostridium difficile, campylobacter jejuni, shigella, salmonella species, cytomegalovirus and amoebae. we describe a case of necrotising haemorrhagic ileo-colitis in a previously fit and healthy young adult female caus ... | 2006 | 16126276 |
| guillain barré syndrome precipitated by the use of antilymphocyte globulin in the treatment of severe aplastic anaemia. | this report describes the case of a 54 year old woman with very severe aplastic anaemia who was treated with antilymphocyte globulin (alg) and developed guillain barré syndrome (gbs). no antecedent infective aetiology was identified. although there are numerous reports of autoimmune disease after treatment with alg in aplastic anaemia, and gbs after immunosuppressive treatment, there are none reporting gbs after the use of alg for severe aplastic anaemia. the occurrence of autoimmune disease aft ... | 2005 | 16126887 |
| the hs:1 serostrain of campylobacter jejuni has a complex teichoic acid-like capsular polysaccharide with nonstoichiometric fructofuranose branches and o-methyl phosphoramidate groups. | recently, the cps biosynthetic loci for several strains of campylobacter jejuni were sequenced and revealed evidence for multiple mechanisms of structural variation. in this study, the cps structure for the hs:1 serostrain of c. jejuni was determined using mass spectrometry and nmr at 600 mhz equipped with an ultra-sensitive cryogenically cooled probe. analysis of cps purified using a mild enzymatic method revealed a teichoic acid-like [-4)-alpha-d-galp-(1-2)-(r)-gro-(1-p](n), repeating unit, wh ... | 2005 | 16128810 |
| comparison of automated microbroth dilution and agar dilution for antimicrobial susceptibility of campylobacter jejuni isolated from dairy sources. | to compare the agreement between microbroth dilution and agar dilution for antimicrobial susceptibility testing of campylobacter jejuni. | 2005 | 16131517 |
| carbohydrate phenotyping of human and animal milk glycoproteins. | breast-milk has a well-known anti-microbial effect, which is in part due to the many different carbohydrate structures expressed. this renders it a position as a potential therapeutic for treatment of infection by different pathogens, thus avoiding the drawbacks of many antibiotics. the plethora of carbohydrate epitopes in breast-milk is known to differ between species, with human milk expressing the most complex one. we have investigated the expression of protein-bound carbohydrate epitopes in ... | 2005 | 16133831 |
| live, attenuated salmonella typhimurium vectoring campylobacter antigens. | we describe the evaluation of three live, attenuated deltaphop/q salmonella enteric serovar typhimurium strains expressing peb1 minus its signal sequence (peb1-ss) from three different plasmids: a pbr-based asd plasmid, an arabinose-based runaway plasmid, which each expressed peb1-ss in the bacterial cytosol, and a peb1::hlya fusion plasmid that directs secretion of peb1-ss into the extracellular milieu. serum igg responses specific for peb1-ss were induced by pbr-derived and runaway plasmids, w ... | 2006 | 16135393 |
| genetic profiling of campylobacter jejuni strains from humans infected in sweden or in thailand, and from healthy swedish chickens, studied by pulsed-field gel electrophoresis (pfge). | the major objective of the study was to explore the genomic diversity between campylobacter jejuni (c.jejuni) from different sources as a tool for epidemiological considerations. subtyping was performed using pulsed-field gel electrophoresis (pfge) and the enzyme used for cleavage was smai. isolates originated from humans infected in sweden (n=49) and thailand (n=32) and from healthy swedish chickens (n=51). eight pfge groups were formed in a dendrogram and 48% of the isolates belonged to 1 of t ... | 2005 | 16138427 |
| development of a multilocus sequence typing scheme for characterization of clinical isolates of acinetobacter baumannii. | in this study a multilocus sequence typing (mlst) scheme for acinetobacter baumannii was developed and evaluated by using 40 clinical a. baumannii isolates recovered from outbreaks in spanish and german hospitals during the years 1990 to 2001, as well as isolates from other european hospitals and two dsmz reference strains of a. baumannii. for comparison, two isolates of acinetobacter species 13 (sensu tjernberg and ursing), two clinical isolates, and three dsmz strains of a. calcoaceticus (both ... | 2005 | 16145081 |
| experimental infection of chickens with campylobacter jejuni: strains differ in their capacity to colonize the intestine. | groups of broiler and layer type chickens (25 to 63 d.o.) were inoculated per os with separate isolates of 10 strains of campylobacter jejuni. nine of the 10 strains were originally isolated from chickens and one from a dog. the dog strain and five of the chicken isolates could be isolated after inoculation, but four strains were not recovered from cloacal swabs for up to 4 to 16 days after inoculation. however, it was possible to isolate c. jejuni from these birds, from cloacal swabs, when they ... | 1999 | 16147549 |
| application of a stool antigen test to evaluate the incidence of helicobacter pylori infection in children and adolescents from tehran, iran. | helicobacter pylori infection is acquired mainly in childhood, especially in developing countries, where a low-cost, rapid diagnostic technique which is reliable for all age groups may be useful for the management of h. pylori infection. for this purpose, we used an hpsa test (equipar) to detect h. pylori infection in children and adolescents from tehran, iran. thirty-five children who were positive or negative for h. pylori infection by endoscopy-based tests were used as positive and negative c ... | 2005 | 16148176 |
| posttranslational protein modification in archaea. | one of the first hurdles to be negotiated in the postgenomic era involves the description of the entire protein content of the cell, the proteome. such efforts are presently complicated by the various posttranslational modifications that proteins can experience, including glycosylation, lipid attachment, phosphorylation, methylation, disulfide bond formation, and proteolytic cleavage. whereas these and other posttranslational protein modifications have been well characterized in eucarya and bact ... | 2005 | 16148304 |
| survival of campylobacter jejuni in water: effect of grazing by the freshwater crustacean daphnia carinata (cladocera). | environmental studies of the human-pathogenic bacterium campylobacter jejuni have focused on linking distributions with potential sources. however, in aquatic ecosystems, the abundance of c. jejuni may also be regulated by predation. we examine the potential for grazing by the freshwater planktonic crustacean daphnia carinata to reduce the survival of c. jejuni. we use a system for measuring grazing and clearance rates of d. carinata on bacteria and demonstrate that d. carinata can graze c. jeju ... | 2005 | 16151090 |
| colonization of cattle intestines by campylobacter jejuni and campylobacter lanienae. | the location and abundance of campylobacter jejuni and campylobacter lanienae in the intestines of beef cattle were investigated using real-time quantitative pcr in two studies. in an initial study, digesta and tissue samples were obtained along the digestive tract of two beef steers known to shed c. jejuni and c. lanienae (steers a and b). at the time of slaughter, steer b weighed 540 kg, compared to 600 kg for steer a, yet the intestine of steer b (40.5 m) was 36% longer than the intestine of ... | 2005 | 16151098 |
| new gammaproteobacteria associated with blood-feeding leeches and a broad phylogenetic analysis of leech endosymbionts. | many monophagous animals have coevolutionary relationships with bacteria that provide unavailable nutrients to the host. frequently, these microbial partners are vertically inherited and reside in specialized structures or tissues. here we report three new lineages of bacterial symbionts of blood-feeding leeches, one from the giant amazonian leech, haementeria ghilianii, and two others from placobdelloides species. these hosts each possess a different mycetome or esophageal organ morphology wher ... | 2005 | 16151107 |
| survival of campylobacter jejuni in waterborne protozoa. | the failure to reduce the campylobacter contamination of intensively reared poultry may be partially due to campylobacter resisting disinfection in water after their internalization by waterborne protozoa. campylobacter jejuni and a variety of waterborne protozoa, including ciliates, flagellates, and alveolates, were detected in the drinking water of intensively reared poultry by a combination of culture and molecular techniques. an in vitro assay showed that c. jejuni remained viable when inter ... | 2005 | 16151149 |
| ca2+ release from host intracellular stores and related signal transduction during campylobacter jejuni 81-176 internalization into human intestinal cells. | campylobacter jejuni is the leading bacterial cause of human diarrhoeal disease in many parts of the world, including the usa. the ability of c. jejuni to invade the host intestinal epithelium is an important determinant of virulence. a common theme among pathogenic invasive micro-organisms is their ability to usurp the eukaryotic cell-signalling systems both to allow for invasion and to trigger disease pathogenesis. ca(2+) is very important in a great variety of eukaryotic cell-signalling proce ... | 2005 | 16151220 |
| biogenesis, architecture, and function of bacterial type iv secretion systems. | type iv secretion (t4s) systems are ancestrally related to bacterial conjugation machines. these systems assemble as a translocation channel, and often also as a surface filament or protein adhesin, at the envelopes of gram-negative and gram-positive bacteria. these organelles mediate the transfer of dna and protein substrates to phylogenetically diverse prokaryotic and eukaryotic target cells. many basic features of t4s are known, including structures of machine subunits, steps of machine assem ... | 2005 | 16153176 |