Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| in vivo kinetics of cytokine expression during peritonitis in carp: evidence for innate and alternative macrophage polarization. | despite the discovery of many cytokine genes in fish, knowledge on their functional homology is limited. to enlighten the biological function of inflammation-related mediators, we studied their kinetics of gene expression during peritonitis in carp. zymosan-induced intraperitoneal influx of phagocytes reached a maximum at 24h. in peritoneal leukocytes (ptl) up-regulation of il-1beta, tnf-alpha, cxca, and chemokine receptor cxcr1 preceded this peak. delayed up-regulation of these genes in the hea ... | 2008 | 17988736 |
| characterization and functional analysis of goldfish (carassius auratus l.) tumor necrosis factor-alpha. | we identified and characterized two isoforms of tumor necrosis factor-alpha (tnfalpha) from the goldfish, tnfalpha-1 and tnfalpha-2. at the protein level, goldfish tnfalpha-1 and tnfalpha-2 were most homologous to carp tnfalpha-1 and tnfalpha-2, respectively. phylogenetically, the two goldfish isoforms grouped most closely with the carp tnfalpha isoforms and tnf species of other cyprinids. real-time pcr analysis revealed constitutive expression of goldfish tnfalpha-1 and tnfalpha-2 in all tissue ... | 2008 | 17988738 |
| studies on fish and pork paste gelation by dynamic rheology and circular dichroism. | the muscle paste of fish, pork, and their mixtures were prepared to study the gelling characteristics by dynamic rheological measurement. the gelation mechanisms of muscle paste were also investigated by circular dichroism. gel formation of fish paste occurred in 2 steps of 5 to 35 and 51 to 90 degrees c respectively, while pork paste mainly in 1 step of 49 to 72 degrees c. gel formation was relative to the alpha-helix unfolding of myosin, which responded the melting temperatures of 40 and 50 de ... | 2007 | 17995642 |
| characterization of teleost phagocyte nadph oxidase: molecular cloning and expression analysis of carp (cyprinus carpio) phagocyte nadph oxidase. | we previously demonstrated that some fish have unique response in the form of reactive oxygen species (ros) production. in the present study, we cloned and sequenced the full-length cdnas for carp (cyprinus carpio) phagocyte nadph oxidase components: gp91phox, p22phox, p47phox, p67phox and p40phox. these amino acid sequences were compared with other teleost and mammalian homologues, to elucidate the features of ros production of fish neutrophils. the phylogeny analysis clearly demonstrates that ... | 2008 | 17997160 |
| food safety aspects of toxic element accumulation in fish from wastewater-fed ponds in hanoi, vietnam. | to evaluate the food safety of fish from production systems in hanoi feed with domestic and industrial wastewater with respect to the potentially toxic elements such as arsenic (as), cadmium (cd) and lead (pb). | 2007 | 18005313 |
| occurrence and species distribution of fishborne zoonotic trematodes in wastewater-fed aquaculture in northern vietnam. | to assess the risk of fishborne zoonotic trematode (fzt) infection in fish reared in wastewater-fed ponds in peri-urban areas of northern vietnam. | 2007 | 18005317 |
| administration of recombinant parasite beta-tubulin to goldfish (carassius auratus l.) confers partial protection against challenge infection with trypanosoma danilewskyi laveran and mesnil, 1904. | the infection of carp and other cyprinid fish with trypanosoma danilewskyi was reported to cause significant morbidity and mortality in aquaculture. tubulin is a component of parasite excretory/secretory (es) products recognized by antibodies present in the serum of recovered hosts. to assess the role of parasite tubulin in the induction of a protective immune response in the goldfish, recombinant t. danilewskyi beta-tubulin was produced in escherichia coli and used to immunize goldfish against ... | 2008 | 18006235 |
| microcystin kinetics (bioaccumulation and elimination) and biochemical responses in common carp (cyprinus carpio) and silver carp (hypophthalmichthys molitrix) exposed to toxic cyanobacterial blooms. | two species of common edible fish, common carp (cyprinus carpio) and silver carp (hypophthalmichthys molitrix), were exposed to a microcystis spp.-dominated natural cyanobacterial water bloom for two months (concentrations of cyanobacterial toxin microcystin, 182-539 microg/g biomass dry wt). toxins accumulated up to 1.4 to 29 ng/g fresh weight and 3.3 to 19 ng/g in the muscle of silver carp and common carp, respectively, as determined by enzyme-linked immunosorbent immunoassay. concentrations a ... | 2007 | 18020691 |
| transcription of signal-3 cytokines, il-12 and ifn alpha beta, coincides with the timing of cd8 alpha beta up-regulation during viral infection of common carp (cyprinus carpio l). | mammalian naïve cd8+ t cells are activated by antigen (signal 1) and cd28 costimulation (signal 2) to undergo several rounds of cell division, but programming for survival, effector function and memory requires a third signal that can be provided by il-12 and/or type i interferons. functional studies indicate that the route of antigen presentation and costimulation are conserved from fish to mammals. however, the potential of il-12 and ifn alpha beta to act as signal-3 cytokines in infections in ... | 2008 | 18022233 |
| the effect of temperature increase on the expression of manganese superoxide dismutase in tissues of common carp cyprinus carpio. | the increase of environmental temperature at physiological range can cause oxidative stress in exotherms, which in most cases leads to activation of the antioxidant enzyme, manganese-1 superoxide dismutase (mn-sod). this work is aimed to evaluate the changes in mn-sod enzyme activity and mrna levels in the common carp, cyprinus carpio, during transition from lower (15 degrees c) to higher (25 and 30 degrees c) temperatures. in liver, 25 degrees c exposure elicited little effect, but at 30 degree ... | 2015 | 18030755 |
| morphogenesis of koi herpesvirus observed by electron microscopy. | koi herpesvirus (khv or cyprinid herpesvirus 3) was inoculated onto three fish cell lines derived from carp, cyprinus carpio l., and the process of virion formation observed with electron microscopy. essentially, similar features of virus particles were observed in all three cell lines. the nucleus of infected cells was characterized by margination of chromatin and often contained many capsids of about 110 nm in diameter with varying morphology. the morphological variation of the capsids was ver ... | 2007 | 18034678 |
| a novel immunoglobulin-like transcript from rainbow trout with two ig-like domains and two isoforms. | within the innate immune response in primates the nonrearranging killer immunoglobulin-like receptors (kir) enable natural killer cells to discern target cells exposing "missing self" signals. recently the novel immune-type receptor (nitr) and the novel immunoglobulin-like transcript (nilt) gene families have been discovered in fish encoding nonrearranging receptors with a similar molecular structure to that of kirs. besides the structural similarity the high degree of haplotypic and allelic var ... | 2008 | 18035417 |
| chemical contaminants, health indicators, and reproductive biomarker responses in fish from rivers in the southeastern united states. | largemouth bass (micropterus salmoides) and common carp (cyprinus carpio) were collected from 13 sites located in the mobile (mrb), apalachicola-flint-chattahoochee (arb), savannah (srb), and pee dee (prb) river basins to document spatial trends in accumulative chemical contaminants, health indicators, and reproductive biomarkers. organochlorine residues, 2,3,7,8-tetrachlorodibenzo-p-dioxin-like activity (tcdd-eq), and elemental contaminants were measured in composite samples of whole fish, grou ... | 2008 | 18036634 |
| microsatellite dna marker analysis of genetic diversity in wild common carp (cyprinus carpio l.) populations. | thirty microsatellite loci were used for analyzing six wild populations of common carp (cyprinus carpio l.). observed (h(o)) and expected (h(e)) heterozygosity values, polymorphic information content (pic), and number of effective alleles (a(e)) were all detected. genetic similarity index and genetic distance were computed based on the allele frequency. the hardy-weinberg equilibrium was checked according to the test of chi2. genetic differentiation and hierarchical partition of genetic diversit ... | 2007 | 18037135 |
| influence of fish on habitat choice of water birds: a whole system experiment. | it is notoriously difficult to study population interactions among highly mobile animals that cannot be meaningfully confined to experimental plots of limited size. for example, migratory water birds are believed to suffer from competition with resident fish populations for shared food resources. while observational evidence in support of this hypothesis is accumulating, replicated experiments addressing this issue at the proper spatial scale are lacking. here, we report from a replicated whole- ... | 2007 | 18051660 |
| a potential role for calcium / calmodulin-dependent protein kinase-related peptide in neuronal apoptosis: in vivo and in vitro evidence. | previously, we have established that a product of the doublecortin-like kinase (dclk) gene, dclk-short, is cleaved by caspases during serum deprivation. subsequently, the n-terminal cleavage product of dclk-short facilitates apoptosis in the neuroblastoma cell line ng108. as this n-terminal cleavage product is highly homologous to calcium/calmodulin-dependent protein kinase-related peptide (carp), another dclk gene splice variant, we aimed to determine the possible apoptotic properties of carp i ... | 2007 | 18052980 |
| depletion of high energy phosphates implicates post-exercise mortality in carp and trout; an in vivo 31p-nmr study. | as in vivo 31p-nuclear magnetic resonance spectroscopy is currently the state of the art method to measure continuously intracellular ph (ph(i)) and energy status of muscle tissue, we used this method to study the recovery from exhaustive exercise. the biochemical changes during recovery are not well understood and it was suggested that post-exercise mortality could be caused by low ph(i); other studies however indicate that energy depletion might be more important. to analyse the mechanism of p ... | 2008 | 18055239 |
| routine turning maneuvers of koi carp cyprinus carpio koi: effects of turning rate on kinematics and hydrodynamics. | spontaneous swimming behaviors of koi carp cyprinus carpio koi were recorded using a video tracking system. routine single-beat turns were selected from the recorded image sequences for kinematic and hydrodynamic analysis. as with c-starts, the turns can be divided into two stages (stage 1 and stage 2), based on kinematics. stage 1 involves a bend to one side forming a c-shaped curve in the body, while stage 2 corresponds to the return flip of the body and tail. the turning angle in stage 1 acco ... | 2007 | 18055627 |
| acute toxicity of synthetic pyrethroid cypermethrin to some freshwater organisms. | ninety-six hours static bioassays were made in the laboratory to determine acute toxicity of cypermethrin to five non-target freshwater organisms belonging to different taxa and niche. susceptibility of the organisms to cypermethrin was in the order: the crustacean diaptomus forbesi > the aquatic insect ranatra filiformis > the freshwater carp cyprinus carpio > the tadpole larva of the toad bufo melanostictus > the oligochaet worm branchiura sowerbyi. ninety-six hours lc50 values of aqueous cype ... | 2008 | 18058051 |
| regulation of amp-deaminase activity from white muscle of common carp cyprinus carpio. | amp-deaminase was purified to electrophoretic homogeneity from white skeletal muscle of a teleost fish, the common carp, cyprinus carpio. the purified enzyme was highly stable and showed non-michaelis-menten kinetics with a s(0.5) value for amp of 2.52+/-0.16 mm (sem) and a hill coefficient of 1.19+/-0.11. specific activity of the purified enzyme was 1000-1200 u/mg protein. the ph optimum was 6.3 and the enzyme was activated by adp and atp, but inhibited by phosphate and fluoride. low concentrat ... | 2008 | 18060819 |
| accumulation and effects of metals in caged carp and resident roach along a metal pollution gradient. | metal accumulation and effects on plasma ca(2+), alanine transaminase (alt) and fish condition factor were examined in caged carp (cyprinus carpio) and resident roach (rutilus rutilus) at four locations along the grote nete river system (belgium). cadmium and zinc accumulation were found in carp and roach, with highest concentrations at the most contaminated site (dissolved cd: 1.82 microg/l, zn: 967 microg/l). on the tissue level, highest cadmium concentrations were measured in kidneys of carp ... | 2008 | 18061244 |
| the changes of serum testosterone level and hepatic microsome enzyme activity of crucian carp (carassius carassius) exposed to a sublethal dosage of pentachlorophenol. | pentachlorophenol (pcp), which was reported to be a typical persistent organic pollutant and environmental endocrine disruptor, may cause threat to aquatic species. in this study, serum testosterone concentration, activity of liver microsome ethoxyresorufin o-deethylase (erod) and glutathione s-transferases (gst) of crucian carp (carassius carassius) exposed to pcp for 7 and 15 d, respectively, were examined. the results showed that testosterone concentration was induced remarkably after 7 d (p< ... | 2008 | 18063032 |
| the effect of starving and feeding on copper toxicity and uptake in cu acclimated and non-acclimated carp. | common carp (cyprinus carpio) were fed two different food rations: 0.5% body weight (low ration, lr) and 5% body weight (high ration, hr) and were either acclimated to sublethal copper (1 microm) for 28 days in softened antwerp city tap water or not acclimated. fish were exposed for 10 days to high cu levels using four different concentrations (3.5, 6, 10, and 15 microm) before and after the cu acclimation. fish tolerance against cu exposure was evaluated, and gill, liver, and carcass cu and sod ... | 2008 | 18063142 |
| impact of orthotopic liver transplant for primary sclerosing cholangitis on chronic antibiotic refractory pouchitis. | the effect of orthotopic liver transplantation (olt) for primary sclerosing cholangitis (psc) and post-olt immunosuppression on the disease course of pouchitis is not clear. the aims of this study were to compare the frequency of chronic antibiotic-refractory pouchitis (carp) in psc patients with or without olt and to assess potential risk factors for carp in these patients. | 2008 | 18065274 |
| [genetic potential analysis of germany mirror carp (cyprinus carpio l.) using microsatellite markers]. | using 30 microsatellite markers and combining quantity characters such as body weight, body size and body width, we evaluated the genetic potential of 3 germany mirror carp populations. number of effective alleles (ae), observed (ho) and expected (he) heterozygosity values and polymorphic information contents (pic) were all calculated. 287 alleles and 559 genotypes were detected. the dna fragment length was 109-400 bp. the hardy-weinberg equilibrium was checked and phenomenon of some disequilibr ... | 2007 | 18065388 |
| detection of cyprinid herpesvirus type 3 in goldfish cohabiting with cyhv-3-infected koi carp (cyprinus carpio koi). | 2007 | 18065817 | |
| effects of astragalus polysaccharides (aps) on the expression of immune response genes in head kidney, gill and spleen of the common carp, cyprinus carpio l. | for fish immune defences, cytokines and anti-microbial peptides (lysozyme) in circulating system play important roles. in the present study, the effects of astragalus polysaccharides (aps) injection on gene expression of interleukin 1beta (il-1beta), tumor necrosis factoralpha (tnf-alpha) and lysozyme-c in the head kidney, gill and spleen of common carp were determined using semi-quantitative reverse transcription-polymerase chain reaction (rt-pcr). after injection of aps, il-1beta mrna level in ... | 2008 | 18068100 |
| genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional orf. | the complete genome of mandarin fish siniperca chuatsi rhabdovirus (scrv) was cloned and sequenced. it comprises 11,545 nucleotides and contains five genes encoding the nucleoprotein n, the phosphoprotein p, the matrix protein m, the glycoprotein g, and the rna-dependent rna polymerase protein l. at the 3' and 5' termini of scrv genome, leader and trailer sequences show inverse complementarity. the n, p, m and g proteins share the highest sequence identities (ranging from 14.8 to 41.5%) with the ... | 2008 | 18068257 |
| characterization of nitric oxidergic neurons in the alimentary tract of the snail helix pomatia l.: histochemical and physiological study. | by using nadph-diaphorase (nadph-d) histochemistry, nitric oxide synthase (nos) immunohistochemistry, western blotting, and no pharmacology, we investigated the distribution and possible function of nos-containing neurons in different units of the alimentary tract of the snail, helix pomatia. discrete populations of neurons in the buccal ganglia displayed nadph-d reactivity. nadph-d-reactive and nos-immunoreactive (nos-ir) neurons were present in the caecum, and labeled fibers were found to inne ... | 2008 | 18076086 |
| evaluation of estrogenicity of sewage effluent and reclaimed water using vitellogenin as a biomarker. | to evaluate the quality of reclaimed water, the estrogenicity of effluent from a sewage treatment plant and of reclaimed water treated with coagulation-sedimentation-filtration using the effluent as raw water was assessed using vitellogenin (vtg) as a biomarker. after a three-week exposure, significant (p < 0.05) induction of vtg occurred in female crucian carp (carassius carassius) exposed continuously to the secondary effluent and reclaimed water with different dilutions (12.5, 25, 50, and 100 ... | 2008 | 18092868 |
| on the occurrence of the mrigal, cirrhina mrigala, hamilton, in the river godavari. | 1949 | 18112242 | |
| detection of cyprinid herpesvirus-3 (cyhv-3) dna in infected fish tissues by nested polymerase chain reaction. | diagnostic tests with high analytical sensitivity are required to detect cyprinid herpesvirus-3 (cyhv-3) in carriers that have been implicated in the dissemination of this important disease of koi and common carp cyprinus carpio. a nested polymerase chain reaction (pcr) test was developed to detect cyhv-3 dna in tissues of infected fish. the 2-round pcr amplified 529 and 379 bp segments of the cyhv-3 gene coding for the major capsid protein using specific external and internal primers, respectiv ... | 2007 | 18159669 |
| non-specific adsorption of fish immunoglobulin m (igm) to blocking reagents on elisa plate wells. | enzyme-linked immunosorbent assay (elisa) is a popular technique for quantifiable detection of specific antibodies in warm-blooded animals, but it has not been accepted for detection of fish antibodies because of its low reproducibility, which is due in part to high background optical density (od) measurements. in the present study, we report that the high background of a fish antibody-detection elisa resulted from non-specific adsorption of fish immunoglobulin m (igm) to blocking reagents on th ... | 2007 | 18159673 |
| molecular cloning and functional characterisation of a cathepsin l-like proteinase from the fish kinetoplastid parasite trypanosoma carassii. | trypanosoma carassii is a fish kinetoplastid parasite that belongs to the family trypanosomatida. in the present study we cloned a cathepsin l-like proteinase from t. carassii. the nucleotide sequence of 1371bp translated into a preproprotein of 456 amino acids. the preproprotein contained the oxyanion hole (gln), the active triad formed by cys, his and asn and the conserved erfnin-like, gnfd and gcngg motifs, characteristic for cathepsin l proteinases. phylogenetic analysis showed that the t. c ... | 2008 | 18162408 |
| the cloning of dmc1 cdnas and a comparative study of its expression in different ploidy cyprinid fishes. | dmc1 (disrupted meiotic cdna) is a functionally specific gene, which was firstly discovered in yeast and then found to encode a protein required for homologous chromosome synapsis during the process of meiosis. in this investigation, we cloned the partial cdnas of dmc1 of diploid red crucian carp, japanese crucian carp, common carp, triploid crucian carp and allotetraploid hybrids by using a pair of degenerate primers based on the conservative sequence of amino acids of the dmc1 protein in yeast ... | 2008 | 18176789 |
| relationship among follicular apoptosis, integrin beta1 and collagen type iv during early ovarian regression in the teleost prochilodus argenteus after induced spawning. | early ovarian regression was analyzed in the neotropical freshwater teleost, curimatã-pacu (prochilodus argenteus), in order to evaluate follicular apoptosis, basement membrane morphology, and integrin beta1 and collagen type iv immunostainning in postovulatory follicles. mature females were induced to spawn by using carp pituitary extract for study of ovarian regression up to 5 days post-spawning. morphometric analyses showed that the postovulatory follicle area decreased progressively after sp ... | 2008 | 18193286 |
| mitochondrial dna analysis reveals cryptic large-scale invasion of non-native genotypes of common carp (cyprinus carpio) in japan. | wild common carp (cyprinus carpio) are probably suffering from biological invasions of conspecific domesticated strains. however, such invasions may be largely camouflaged by morphological similarities between introduced and native strains. we conducted a large survey of mitochondrial dna sequences (complete d-loop region) from 11 localities in japan. from a total of 166 individuals, 28 haplotypes were determined to fit into six divergent clades. one of the six clades included 19 closely related ... | 2008 | 18194166 |
| treatment of ichthyophthiriasis in rainbow trout and common carp with common and alternative therapeutics. | the goal of this laboratory study was to provide better knowledge about the treatment of ichthyophthiriasis (causative agent: ichthyophthirius multifiliis, a ciliate bacteria) in rainbow trout oncorhynchus mykiss and common carp cyprinus carpio. the following questions were investigated: (1) the effectiveness of different chemicals (formalin, sodium chloride, hydrogen peroxide, perotan, virkon, aquahumin, baycox, and ivomec) and at different concentrations and durations of application, (2) the n ... | 2007 | 18201060 |
| effects of sodium dodecylbenzenesulfonate on uptake of pyrene by fish gills. | laboratory experiments were carried out to study the effects of anionic surfactant sodium dodecylbenzenesulfonate (sdbs) on the uptake of pyrene by fish gills. the uptake (including adsorption) of pyrene by gills of the color carp (c. carpio var. color) exposed to 20-100 microg/l pyrene in the presence or absence of 0.2-6.0 mg/l sdbs were determined. the sorption of pyrene by mucus was preliminary studied using a four-step sequential extraction procedure. fish gills were extracted with h(2)o, 0. ... | 2008 | 18205055 |
| effect of long-term exposure to simazine on brain and muscle acetylcholinesterase activity of common carp (cyprinus carpio). | several water-contamination incidents with simazine have occurred in the province of badajoz (spain), due to its excessive use for controlling weeds in olive trees and vineyards. simazine residues were also detected in drinking water, increasing public health concern. however, little is known on the effects that low levels of simazine pose to environment organisms. we investigated if residues of simazine in the natural waters would affect brain and muscle acetylcholinesterase activity in common ... | 2008 | 18214903 |
| acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp carassius auratus. | recent evidences suggested that oxidative stress may play a significant role in the pathogenesis of mcs toxicity. in the present study, the acute effects of microcystins on the transcription of antioxidant enzyme genes were investigated in liver of crucian carp i.p.-injected with 50 mug mc-lreq per kg body weight (bw). we reported the cdna sequences for four kinds of antioxidant enzyme (gsh-px, cat, cu/zn sod, and gr) genes, and evaluated the oxidant stress induced by mcs through analyzing the t ... | 2008 | 18214919 |
| predictors and outcomes of a perioperative myocardial infarction following elective vascular surgery in patients with documented coronary artery disease: results of the carp trial. | the predictors and outcomes of patients with a peri-operative elevation in cardiac troponin i above the 99th percentile of normal following an elective vascular operation have not been studied in a homogeneous cohort with documented coronary artery disease. | 2008 | 18245121 |
| stimulation of salmon calcitonin on secretion of 17beta-estradiol by the ovarian follicles of common carp, cyprinus carpio. | the effects of salmon calcitonin (sct) on the secretion of 17beta-estradiol (e(2)) were examined in female common carp, cyprinus carpio. vitellogenic stage fish adapted to high-ca water were i.p. injected with vehicle, sct, human chorionic gonadotropin (hcg), or hcg plus sct. to determine whether ovarian follicles are equipped with ct receptors, a ct binding assay was conducted. in the in vitro experiments, vitellogenic follicles were incubated with stimulators and inhibitors. administration of ... | 2008 | 18252964 |
| [peculiarities of distribution of plasma proteins between the internal medium specialized compartments in the carp cyprinus carpio]. | 2008 | 18265563 | |
| temporal and geographic trends in mercury concentrations in muscle tissue in five species of hudson river, usa, fish. | we analyzed a new york (usa) state database of mercury concentrations in muscle tissue for five species of fish (striped bass, yellow perch, largemouth bass, smallmouth bass, and carp) over a range of locations in the hudson river (usa) between 1970 and 2004. we used regression models to discern temporal and geographic change in the fish while controlling for a positive correlation between mercury concentration and body mass. mercury concentrations significantly increased in fish from new york h ... | 2008 | 18266478 |
| molecular cloning and characterization of common carp (cyprinus carpio l.) tcrgamma and cd3gamma/delta chains. | partial cdna sequences of tcrgamma and cd3gamma/delta were isolated from the thymus of common carp (cyprinus carpio l.) by the method of suppression subtractive hybridization (ssh). subsequently the full length cdnas of carp tcrgamma and cd3gamma/delta were obtained by means of 3' race and 5' race, respectively. the full length of carp tcrgamma chain is 1368bp and encodes 326 amino acids including a signal peptide region of 19 amino acids and a transmembrane region of 23 amino acids at the c-ter ... | 2008 | 18272397 |
| transcriptional deregulation and a missense mutation define ankrd1 as a candidate gene for total anomalous pulmonary venous return. | total anomalous pulmonary venous return (tapvr) is a congenital heart defect in which the pulmonary veins fail to enter the left atrium and drain instead into the right atrium or one of its venous tributaries. although a genetic basis for tapvr has long been recognized, no single gene involved in the pathogenesis of this disease has been identified to date. we previously reported a tapvr patient bearing a de novo 10;21 balanced translocation. in this work, we cloned both translocation breakpoint ... | 2008 | 18273862 |
| binding kinetics and sequencing of hepatic alpha1-adrenergic receptors in two marine teleosts, mackerel (scomber scombrus) and anchovy (engraulis encrasicolus). | liver alpha(1)-adrenoceptors (ars) are demonstrated, or at least hypothesized, in freshwater and brackish-water teleosts, whereas no data are available for marine teleosts. this study evaluates the presence of alpha(1)-ars in the liver of two marine teleosts, the anchovy engraulis encrasicolus and the mackerel scomber scombrus, and examines on a broad scale the possibility that habitats posing different challenges also influence phenotypic trait selection. binding assays were performed also on l ... | 2008 | 18273865 |
| expression of warm temperature acclimation-related protein 65-kda (wap65) mrna, and physiological changes with increasing water temperature in black porgy, acanthopagrus schlegeli. | we isolated the warm temperature acclimation-related protein 65-kda (wap65) cdna from the liver of black porgy and investigated the expression by increasing water temperature in black porgy, acanthopagrus schlegeli. black porgy wap65 full-length cdna consists of 1,338 nucleotides, including an open reading frame, predicted to encode a protein of 425 amino acids and showed high homology to pufferfish (79%), medaka (73%), carp (70%), and goldfish (68%) wap65. increase in water temperature (20 degr ... | 2008 | 18273879 |
| bioaccumulation of heavy metals in fishes from taihu lake, china. | the cr, zn, cu, cd, pb contents were determined in cyprinus carpio linnaeus, carassius auratus linnaeus, hypophthalmichthys molitrix and aristichthys nobilis, which were caught from meiliang bay, taihu lake, a large, shallow and eutrophic lake of china. the results showed that: (1) the cr, cu, pb, cd contents in the edible parts of the four fish species were much lower than chinese food health criterion (1994), but the zn contents were higher than the criterion; (2) cd contents were the highest ... | 2007 | 18277656 |
| myxobolus infections of common carp (cyprinus carpio) in syrian fish farms. | during a survey on myxobolus infection of pond-cultured common carp in syria three myxobolus spp. were found. myxobolus dispar infected the gill arteries, forming large elongated plasmodia in the gill filaments. the plasmodia of m basilamellaris were located in the gill arches at the base of the filaments. elongated filiform plasmodia of m. encephalicus were found in the blood vessels of the brain. despite the common occurrence of the above parasites, no disease symptoms were observed in the inf ... | 2007 | 18277709 |
| [kinetics of hemoglobin carrying and releasing oxygen.]. | the purpose of the present study was to examine the kinetic process of hemoglobin (hb) carrying and releasing oxygen. under the standard conditions (ph 7.4, p(o2) 20 mmhg, 20 degrees c) the blood samples of chicken, rabbit, frog and carp were equilibrated in oxygen content analyzer with calibrated gas mixture a (0.5% co2 and 99.5% n2). then the blood samples were exposed to gas mixture b (21% o2, 0.5% co2 and 78.5% n2). after equilibration, the blood samples were exposed to gas mixture a again. ... | 2008 | 18288362 |
| localization of zinc in the outer retina of carp: a light- and electron-microscopic study. | many lines of evidence suggest that zinc may play an important neuromodulatory role in the central nervous system, including the retina. in this work, localization of zinc in the outer retina of carp was studied, using the silver amplification method, by light and electron microscopy. reaction products (silver grains) were widely distributed throughout the retina, including photoreceptors, the outer and inner nuclear layers (onl and inl), the ganglion cell layer (gcl), as well as in both outer a ... | 2008 | 18288649 |
| effect of endotoxin on the immunity of indian major carp, labeo rohita. | endotoxin, a lipopolysaccharide component of outer cell wall membrane of the gram-negative bacteria is a factor responsible for a number of biological effects including immunostimulatory activities in different animal species including fish. in this study, l. rohita yearlings of weight ranging from 80 to 100g were injected intraperitoneally with 0.5, 1, 2, 5, 10 and 20 eu/fish dose of endotoxin to find out its effect on the immunity. the l. rohita yearlings were found to resist the endotoxin dos ... | 2008 | 18289877 |
| purification and characterization of gelatinase-like proteinases from the dark muscle of common carp (cyprinus carpio). | gelatinolytic proteinases from common carp dark muscle were purified by 30-60% ammonium sulfate fractionation and a combination of chromatographic steps including ion exchange on deae-sephacel, gel filtration on sephacryl s-200, ion exchange on high-q, and affinity on gelatin-sepharose. the molecular masses of these proteinases as estimated by sds-page were 75, 67, and 64 kda under nonreducing conditions. the enzymes revealed high activity at a slightly alkaline ph range, and their activities we ... | 2008 | 18293920 |
| cloning and expression of carp cathepsin z: possible involvement in yolk metabolism. | a cdna encoding cathepsin z (ctpz) was cloned from a carp ovarian cdna library. it is homologous to mammalian ctpz. the amino acid residues important for protein folding and enzymatic activity of mammalian ctpz are conserved in carp ctpz. it is widely expressed in a variety of carp tissues as revealed by western blot and reverse transcription-polymerase chain reaction. the ctpz mrna was transiently accumulated during oocyte maturation. in oocytes, ctpz is localized in cortical granules and in th ... | 2008 | 18294892 |
| improved normalization of real-time reverse transcriptase polymerase chain reaction data using an external rna control. | no golden standard exists for normalization of real-time reverse transcriptase polymerase chain reaction (rt pcr) data and procedures used are often not validated. numerous studies have indicated that current approaches are inadequate. here, we report the development of an external rna control approach. it is the first to add external rna to tissue on a per unit weight basis, and we demonstrate its accuracy, suitability, and necessity in experiments involving severe physiological challenges. we ... | 2008 | 18294949 |
| a quantitative study of testicular germ cell populations in masculinized neomale common carp (cyprinus carpio l.). | the main objective of the present study was to investigate the effects of 17 alpha-methyltestosterone treatment upon the testicular germ cells of gynogenetic masculinized neomale common carp (cyprinus carpio l.) in comparison with diploid carp. gynogenetic common carp progeny (mean body weight, bw, 2.6+/-0.3g; mean total length, 10.4+/-0.5 cm) were treated for a period of 40 days with 17 alpha-methyltestosterone (mt) at a dose of 100mg kg(-1). the oral administration of mt resulted in 61.5-100% ... | 2008 | 18299213 |
| chronic fluoxetine treatment induces brain region-specific upregulation of genes associated with bdnf-induced long-term potentiation. | several lines of evidence implicate bdnf in the pathogenesis of stress-induced depression and the delayed efficacy of antidepressant drugs. antidepressant-induced upregulation of bdnf signaling is thought to promote adaptive neuronal plasticity through effects on gene expression, but the effector genes downstream of bdnf has not been identified. local infusion of bdnf into the dentate gyrus induces a long-term potentiation (bdnf-ltp) of synaptic transmission that requires upregulation of the imm ... | 2007 | 18301726 |
| phenotypic plasticity in gut length in the planktivorous filter-feeding silver carp (hypophthalmichthys molitrix). | phenotypic plasticity widely exists in the external morphology of animals as well as the internal traits of organs. in the present study, we studied the gut length plasticity of planktivorous filter-feeding silver carp under different food resources in large-net cage experiments in meiliang bay of lake taihu in 2004 and 2005. there was a significant difference in stocking density between these 2 years. under a low stocking density and abundant food resources, silver carp increased their energy i ... | 2008 | 18301818 |
| cloning, characterization and promoter analysis of the common carp igf-ii gene. | insulin-like growth factors (igfs) belong to a family of growth factors with structural homology to proinsulin. up till now, no specific details regarding the transcriptional regulation by autocrine, paracrine or endocrine effector molecules in vivo have been described for the igf-ii gene. this is in big contrast to igf-i gene transcription which has been studied more extensively. to better understand how the igf-ii gene is controlled at the gene transcription level, we have isolated the common ... | 2008 | 18304762 |
| seasonal acclimatization of brain lipidome in a eurythermal fish (carassius carassius) is mainly determined by temperature. | crucian carp (carassius carassius) is an excellent vertebrate model for studies on temperature adaptation in biological excitable membranes, since the species can tolerate temperatures from 0 to +36 degrees c. to determine how temperature affects the lipid composition of brain, the fish were acclimated for 4 wk at +30, +16, or +4 degrees c in the laboratory, or seasonally acclimatized individuals were captured from the wild throughout the year (temperature = +1 to +23 degrees c), and the brain g ... | 2008 | 18305022 |
| managing the koi herpesvirus disease outbreak in indonesia and the lessons learned. | in 2002, the suspected koi herpesvirus (khv) outbreak in indonesia was investigated by an international emergency disease control task force organized by naca immediately following a request for assistance by the government of indonesia. the task force gained immediate support from aciar, aahri, fao, centex-thailand, intervet, stirling university, and the university of california. the task force findings revealed the involvement of an infectious agent, an analogy with khv outbreaks, its introduc ... | 2007 | 18306515 |
| gata3 mrna in ginbuna crucian carp (carassius auratus langsdorfii): cdna cloning, splice variants and expression analysis. | gata3, a transcriptional activator, plays a critical role in the development of t-cells and differentiation to t helper type 2 cells. to date, no information is available on the role of gata3 in the teleost immune system. we identified full-length cdna and alternatively spliced variants of ginbuna crucian carp gata3 (gbgata3). the gbgata3 gene is transcribed into multiple splice variants lacking either one or both zinc finger domains, although the sequences of both domains are fully conserved be ... | 2008 | 18313140 |
| monitoring of pesticide chlorpyrifos residue in farmed fish: investigation of possible sources. | chlorpyrifos, a widely used organophosphorus insecticide having many urban and agricultural pest control uses, is one of the major pesticides detected in taiwan fishery products. whereas previous studies examined, this study explored possible sources of chlorpyrifos residue, particularly in farmed fish. eight hundred fourteen samples of marketable fish were analyzed for chlorpyrifos residues. one hundred thirty-seven samples contained detectable residues, and farmed fish showed higher detection ... | 2008 | 18313724 |
| long-term hematopoietic reconstitution by transplantation of kidney hematopoietic stem cells in lethally irradiated clonal ginbuna crucian carp (carassius auratus langsdorfii). | the study of hematopoietic stem cells (hscs) has been facilitated by the transplantation of bone marrow cell populations into lethally irradiated mice. it is widely known that hscs have the capacity for long-term and multilineage hematopoietic reconstitution in lethally irradiated hosts. here, we developed a transplantation model system using clonal ginbuna crucian carp (carassius auratus langsdorfii) that were exposed to a lethal dose of x-rays. the minimum lethal dose (mld) of ginbuna was appr ... | 2008 | 18314191 |
| functionality of a bacillus cereus biological agent in response to physiological variables encountered in aquaculture. | the potential of a bacillus cereus isolate (nrrl 100132) as a biological agent for aquaculture has been demonstrated in vitro and in vivo. the functionality of this isolate across a range of physiological conditions, including salinity, ph and temperature, based on rearing of high-value ornamental cyprinus carpio, was investigated. temperature had a significant influence on germination, specific growth rate and increase in cell number of b. cereus in shake-flask cultures, whilst salinity and ph ... | 2008 | 18317748 |
| sexually disrupting effects of nonylphenol and diethylstilbestrol on male silver carp (carassius auratus) in aquatic microcosms. | based on detected nonylphenol (np) levels in aquaculture water, this study investigated sexually disrupting effects in mature male silver carp (carassius auratus) exposed to np and a positive control diethylstilbestrol (des). the combined evidences of steroid hormone (17beta-estradiol, estrone and testosterone) levels and hispathological pictures showed that np (10 microg/l) and des could exert estrogenic effects through indirect mechanisms [i.e. increased estrogens levels (up to two times) and ... | 2008 | 18325589 |
| comparison of taurine biosynthesis ability between juveniles of japanese flounder and common carp. | this study was conducted to investigate taurine deficiency and the ability of taurine biosynthesis in both juvenile japanese flounder (jf) and juvenile common carp (cc) in vivo using low taurine level diets. three different taurine level diets were prepared by the supplementation of taurine to the basal composition (jf--0, 0.5 and 1.5% in jf; cc--0, 1, 3% in cc). the final average body weight and feed efficiency of jf fed the jf - 1.5% was significantly higher than those of fish fed on the jf--0 ... | 2008 | 18327631 |
| a liposome immune lysis assay for enrofloxacin in carp and chicken muscle. | a homogenous complement-mediated liposome immune lysis assay (lila) was developed for the determination of enrofloxacin (enro) in carp and chicken muscle. enro was covalently coupled to dppe, and then immobilized onto the surface of liposomes by reverse-phase evaporation method. the performed liposome would be specifically lysed by the sequential additions of anti-enro monoclonal antibody (mab) and guinea pig complement. through a competitive assay format, the performed liposome can be used to d ... | 2008 | 18331861 |
| [analysis of genetic structure of mirror carp population and correlation of microsatellite markers and economic traits]. | the genetic structure of one breeding population of mirror carp(cyprinus carpio l.) was analysed using thirty-five polymorphic microsatellite markers. the effective number of alleles (ae), observed heterozygosity (ho), expected heterozygosity (he) and polymorphism information content(pic) were all determined. the hardy-weinberg equilibrium was checked by chi-square test. for each locus, 2-7 alleles were detected, with a total of 118 alleles for 35 loci . the value of ae, ho, he and pic showed th ... | 2008 | 18332007 |
| identification of differentially expressed genes in carp rods and cones. | rods and cones differ in their photoresponse characteristics, morphology, and susceptibilities to certain diseases. to contribute to the studies at the molecular level of these differences, we tried to identify genes expressed preferentially in rods or cones. | 2008 | 18334952 |
| cloning of the koi herpesvirus genome as an infectious bacterial artificial chromosome demonstrates that disruption of the thymidine kinase locus induces partial attenuation in cyprinus carpio koi. | koi herpesvirus (khv) is the causative agent of a lethal disease in koi and common carp. in the present study, we describe the cloning of the khv genome as a stable and infectious bacterial artificial chromosome (bac) clone that can be used to produce khv recombinant strains. this goal was achieved by the insertion of a loxp-flanked bac cassette into the thymidine kinase (tk) locus. this insertion led to a bac plasmid that was stably maintained in bacteria and was able to regenerate virions when ... | 2008 | 18337580 |
| comparison of antimicrobial activity in the epidermal mucus extracts of fish. | the mucus layer on the surface of fish consists of several antimicrobial agents that provide a first line of defense against invading pathogens. to date, little is known about the antimicrobial properties of the mucus of arctic char (salvelinus alpinus), brook trout (s. fontinalis), koi carp (cyprinus carpio sub sp. koi), striped bass (morone saxatilis), haddock (melanogrammus aeglefinus) and hagfish (myxine glutinosa). the epidermal mucus samples from these fish were extracted with acidic, orga ... | 2008 | 18342561 |
| a 15mb duplication of 6q24.1-q25.3 associated with typical but milder features of the duplication 6q syndrome. | we report on a female patient carrying a de novo 15mb duplication of 6q24.1-q25.3 detected by conventional karyotyping and fine-mapped by molecular karyotyping with a 250k snp array. pure interstitial duplications of 6q are rarely reported in the literature and none of them exactly mapped by array technique so far. our patient shows typical aspects of the "duplication 6q" syndrome such as hypertelorism, downslanting palpebral fissures, carp shaped mouth and joint contractures, but milder mental ... | 2008 | 18342594 |
| identification of envelope protein porf81 of koi herpesvirus. | koi herpesvirus (khv), an emerging pathogen causing mass mortality in koi and common carp, possesses the largest known herpesvirus genome of 295 kbp predicted to encode 156 different proteins. however, none of them has been identified or functionally characterized up to now. in this study, a rabbit antiserum was prepared against a bacterial fusion protein that permitted detection of the predicted type iii membrane protein encoded by orf81 of khv. in western blot analyses, the abundant orf81 gene ... | 2008 | 18343829 |
| the effect of hypoxia on gill morphology and ionoregulatory status in the lake qinghai scaleless carp, gymnocypris przewalskii. | goldfish and crucian carp at low temperature exhibit plasticity in gill morphology during exposure to hypoxia to enhance gas exchange. hypoxia-induced changes in gill morphology and cellular ultrastructure of the high altitude scaleless carp from lake qinghai, china, were investigated to determine whether this is a general characteristic of cold water carp species. fish were exposed to acute hypoxia (0.3 mg o2 l(-1)) for 24 h followed by 12 h recovery in normoxic water (6 mg o2 l(-1) at 3200 m a ... | 2008 | 18344480 |
| detection of spring viraemia of carp virus (svcv) by loop-mediated isothermal amplification (lamp) in koi carp, cyprinus carpio l. | spring viraemia of carp virus (svcv) is a rhabdovirus associated with systemic illness and mortality in cyprinids. several diagnostic tests are available for detection of svcv. however, most of these tests are time consuming and are not well adapted for field-based diagnostics. in this study, a diagnostic tool for svcv detection based on reverse transcription loop-mediated isothermal amplification (rt-lamp) has been developed. based on the nucleotide sequence of the glycoprotein (g) gene of svcv ... | 2008 | 18353016 |
| structure, organization and expression of common carp (cyprinus carpio l.) slp-76 gene. | slp-76 is an important member of the slp-76 family of adapters, and it plays a key role in tcr signaling and t cell function. partial cdna sequence of slp-76 of common carp (cyprinus carpio l.) was isolated from thymus cdna library by the method of suppression subtractive hybridization (ssh). subsequently, the full length cdna of carp slp-76 was obtained by means of 3' race and 5' race, respectively. the full length cdna of carp slp-76 was 2007 bp, consisting of a 5'-terminal untranslated region ... | 2008 | 18353678 |
| chemotaxis towards, adhesion to, and growth in carp gut mucus of two aeromonas hydrophila strains with different pathogenicity for common carp, cyprinus carpio l. | characteristics that promote bacterial colonization of the intestinal mucosal surface were examined in two strains of the common fish pathogen aeromonas hydrophila, with different pathogenicity. the characteristics examined were chemotactic activity towards mucus, bacterial adherence to mucus and growth in mucus. intestinal gut mucus of healthy common carp was used. the results indicate that chemotaxis is not necessary for a bacterium to become pathogenic, but it may be a necessary parameter for ... | 2008 | 18355183 |
| expression of the polymeric immunoglobulin receptor (pigr) in mucosal tissues of common carp (cyprinus carpio l.). | the mucosal immune system seems to be an important defence mechanism for fish but the binding of igm in mucosal organs is poorly described in fish. in this study the gene encoding the polymeric immunoglobulin receptor (pigr) in carp has been isolated and sequenced from a liver cdna-library and aligned with other species. the pigr of carp consists of 2 ig domains, a transmembrane and an intracellular region, together 327 amino acids. in situ hybridisations with sense and anti-sense dig-labelled p ... | 2008 | 18356080 |
| isolation and characterization of immunoglobulin of the indian major carp, rohu [labeo rohita (ham.)]. | information on the structure and character of immunoglobulin of fishes is essential in health management. a study was carried out to characterize the serum immunoglobulin (igm) of the indian major carp, rohu labeo rohita (ham.). rohu (500g) were immunised with bovine serum albumin (bsa) and the anti-bsa antibody was purified employing bsa-cl agarose affinity column chromatography. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of the purified ig in a 3% gel under non-reduce ... | 2008 | 18375144 |
| development and characterization of monoclonal antibodies to spring viraemia of carp virus. | five monoclonal antibodies (mabs) against spring viraemia of carp (svcv0504, isolated from common carp in china) were produced from mice immunized with purified virus preparations. the virion of svcv contains five structural proteins, representing the nucleoprotein (n), phosphoprotein (p), matrix protein (m), glycoprotein (g) and rna-dependent rna polymerase (l). western blotting analysis revealed that three mabs (1h5, 1e10, and 1h7) recognized specifically to a single protein of 47kda (n), the ... | 2008 | 18378003 |
| heavy metal accumulation in water, sediments and fishes of nallihan bird paradise, turkey. | the accumulation of some heavy metals such as pb, cd, cu and ni was determined in water sediment and fish samples (albumus escherichii, cyprinus carpio and silurus glanis) collected from nallihan bird paradise (nbp) and its vicinity (sariyar dam). the results showed that these metals are found widespread throughout the study area, but metal concentrations in the water samples are below the detection limits (bdl). pb, cd, cu and ni contamination were determined in sediments and in fish tissues (m ... | 2007 | 18380073 |
| impact of low dose of organophosphate, monocrotophos on the epithelial cells of gills of cyprinus carpio communis linn.--sem study. | the paper deals with the deleterious changes at ultrastructural level of the epithelial cells of gills of cyprinus carpio communis linn. upon exposure to 1/10th of lc50 of monocrotophos which is considered to be insignificant concentration from toxicological point of view. the gills of the fish are the primary corridor formolecularexchange between the internal milieu of a fish and its environment. gills perform numerous functions such as oxygen uptake and co2 excretion, osmoregulation, acid-basi ... | 2007 | 18380092 |
| free cyanide-induced biochemical changes in nitrogen metabolism of the indian major carp cirrhinus mrigala. | we studied the effect of free cyanide on several aspects of nitrogen metabolism in the gills, liver, and muscle tissue of the indian major carp, cirrhinus mrigala. free amino acid levels and protease activity were elevated following exposure to lethal and sublethal doses of free cyanide. at the lethal concentration, significant changes were noticed on day 4 for both parameters. the normal condition was restored only at the sublethal concentration. | 2007 | 18380169 |
| visual, lateral line, and auditory ascending pathways to the dorsal telencephalic area through the rostrolateral region of the lateral preglomerular nucleus in cyprinids. | fiber connections of the rostrolateral region of the lateral preglomerular nucleus (pglr) were studied by tract-tracing methods in carp and goldfish. the pglr receives fibers from the optic tectum, ventrolateral nucleus of semicircular torus, ventromedial thalamic nucleus, medial pretoral nucleus, anterior tuberal nucleus, subglomerular nucleus, and (unexpectedly) also from the retina. dendritic morphology of tecto-preglomerular neurons suggests that they receive retinal inputs. the pglr can be ... | 2008 | 18381599 |
| carps enhance p53 turnover by degrading 14-3-3sigma and stabilizing mdm2. | carp1 and carp2 proteins (carps) are e3 ligases that target p53 as well as phospho-p53 for degradation. because mdm2 is a critical regulator of p53 turnover, we investigated and found that carps associate with mdm2. we provide evidence that carps stabilize mdm2 by inhibiting mdm2 self-ubiquitination. carps together with mdm2 enhance p53 degradation, thereby inhibiting p53-mediated cell death. carp protein levels correlate with mdm2 levels including under hypoxia where both are reduced. carp2 was ... | 2008 | 18382127 |
| intracellular calcium concentration changes initiated by n-methyl-d-aspartic acid receptors in retinal horizontal cells. | intracellular calcium concentration changes initiated by n-methyl-d-aspartic acid receptors were studied in carp retinal horizontal cells. fura-2 fluorescent calcium imaging showed that h1 subtype horizontal cells responded to exogenously applied n-methyl-d-aspartic acid with a transient intracellular free ca(2+) ([ca2+]i) increase that decayed to a sustained, but elevated level of [ca(2+)]i. contributions of different ca(2+) flux pathways underlying the time course of this increase in [ca(2+)]i ... | 2008 | 18382285 |
| exposure of mother-child and postpartum woman-infant pairs to ddt and its metabolites in tianjin, china. | 1,1,1-trichloro-2,2'-bis(4-chlorophenyl)ethane (ddt) exhibits its long persistence in the environment, unusual bioaccumulation, effects on wildlife, and the possibility of long-term adverse effects on human health, especially reproductive toxicity. despite the prohibition of most persistent organochlorine pesticides in china, the presence of organochlorine residue, including ddt, has been widely indicated in environmental substance. however, scarce information is available about accumulative lev ... | 2008 | 18387653 |
| vitellogenin, a biomarker for environmental estrogenic pollution, of reeves' pond turtles: analysis of similarity for its amino acid sequence and cognate mrna expression after exposure to estrogen. | vitellogenin (vtg), a biomarker for environmental estrogenic pollution, can be detected in the bloodstream of oviparous animals before morphological and functional abnormalities appear due to exposure to environmental estrogens. reports observing vtg in turtles have been limited. we therefore cloned and sequenced a partial cdna of vtg in reeves' pond turtle, chinemys reevesii. the cloned cdna fragment possessed the start codon and 2,229 bp, encoding 743 amino acid residues. a sequence of deduced ... | 2008 | 18388421 |
| pathogenic potential of a collagenase gene from aeromonas veronii. | the role of collagenase as a mechanism of bacterial pathogenicity in some pathogenic bacteria has been reported. the information on the role of collagenase in aeromonas spp. pathogenesis is scant. in the present study, a mutant aeromonas veronii ry001 that is deficient in the putative collagenase gene acg was constructed and compared with the wild-type strain for virulence factors. bacterial cells and cell-free extracellular products of the mutant had significantly less collagenolytic activity, ... | 2008 | 18388966 |
| biochemical alterations in juvenile carp (cyprinus carpio) exposed to zinc: glutathione reductase as a target. | the aim of this study was to investigate biochemical changes in juvenile carp (cyprinus carpio) exposed to zinc chloride (10, 30 and 100 microm) for a period of 48 h. zinc exposure caused a concentration-dependent reduction in glutathione reductase (gr) activity in gills, liver and brain. gill glutathione s-transferase (gst) was reduced when animals were exposed to the highest concentration of 100 microm zinc. the phosphorylation of p38(mapk) increased in the brain of fish exposed to zinc 100 mi ... | 2008 | 18395787 |
| increased permeability of the blood-brain barrier following administration of glyceryl trinitrate in common carp (cyprinus carpio l.). | nitric oxide (no) has been implicated in the pathogenesis of migraine and treatment with its exogenous donor glyceryl trinitrate (gtn) represents widely accepted experimental "migraine model". in this study, glyceryl trinitrate was administered intraperitoneally to carps, serum nitrite and nitrate levels were determined, permeability of blood-brain barrier was investigated, and histological changes of brain tissue were analyzed. serum nitrite and nitrate levels displayed characteristic biphasic ... | 2008 | 18405066 |
| differential contribution of neutrophilic granulocytes and macrophages to nitrosative stress in a host-parasite animal model. | tyrosine nitration is a hallmark for nitrosative stress caused by the release of reactive oxygen and nitrogen species by activated macrophages and neutrophilic granulocytes at sites of inflammation and infection. in the first part of the study, we used an informative host-parasite animal model to describe the differential contribution of macrophages and neutrophilic granulocytes to in vivo tissue nitration. to this purpose common carp (cyprinus carpio) were infected with the extracellular blood ... | 2008 | 18406465 |
| strain-associated virulence factors of streptococcus iniae in hybrid-striped bass. | streptococcus iniae is a major fish pathogen producing invasive infections that result in economic losses in aquaculture. development of in vitro models of s. iniae virulence may provide insight to the pathogenesis of infection in vivo. three s. iniae strains (k288, 94-426, and 29178) were tested for virulence in a hybrid-striped bass (hsb) model using intraperitoneal injection. s. iniae strains k288 and 94-426 caused high levels of mortality in hsb (lethal dose 2x10(5)cfu) while strain 29178 wa ... | 2008 | 18406547 |
| genome evolution trend of common carp (cyprinus carpio l.) as revealed by the analysis of microsatellite loci in a gynogentic family. | genome evolution arises from two main ways of duplication and reduction. fish specific genome duplication (fsgd) may have occurred before the radiation of the teleosts. common carp (cyprinus carpio l.) has been considered to be a tetraploid species, because of its chromosome numbers (2n=100) and its high dna content. using 69 microsatellite primer pairs, the variations were studied to better understand the genome evolution (genome duplication and diploidization) of common carp from a gynogenetic ... | 2008 | 18407057 |
| in vitro biotransformation of surfactants in fish. part i: linear alkylbenzene sulfonate (c12-las) and alcohol ethoxylate (c13eo8). | developing regulatory activities (e.g., reach, [dgee. 2003. directorates general enterprise and environment. the new eu chemicals legislation reach. dg enterprise, brussels, belgium. (http://www.europa.eu.int/comm/enterprise/reach/index_en.htm)]) will require bioaccumulation to be assessed for thousands of chemicals. further, there is increasing pressure to reduce, refine or replace animal tests. given this scenario, there is an urgent need to evaluate the feasibility of in vitro systems to supp ... | 2008 | 18407314 |
| characterization of the cell-mediated cytotoxic responses of isogeneic ginbuna crucian carp induced by oral immunisation with hapten-modified cellular antigens. | a better understanding of the immune responses in fish elicited by oral immunisation is of importance for the development of new and effective oral vaccines for cultured fish. in the present study, we characterized specific cell-mediated cytotoxic responses in isogeneic ginbuna crucian carp (carassius auratus langsdorfii) following oral immunisation with cellular antigens. trinitrophenyl- (tnp) or dinitrophenyl- (dnp) modified syngeneic and allogeneic cells were used for studying the fine specif ... | 2008 | 18407518 |
| [use of properties and regulation peculiarities of enzymes of glycogenolysis in fish skeletal muscle depending on peculiarities of motor activity of species]. | levels of activity, properties, and peculiarities of activation of glycogen phosphorylase (gp; ec 2.4.1.1) and glycogen phosphorylase kinase (gpk; ec 2.7.1.38) were studied in the white skeletal muscle of fish differing in motor behavior. no differences in the gp and gpk activity levels were revealed in laskir diplodus annularis (l.), horse mackerel trachurus mediterraneus ponticus, salmon salmo trutta morphario, scorpena scorpaena porcus, scophtalnus maeoticus, and carp cyprinus carpio; however ... | 2008 | 18411509 |