Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| h-ns represses inv transcription in yersinia enterocolitica through competition with rova and interaction with ymoa. | yersinia enterocolitica is able to efficiently invade peyer's patches with the aid of invasin, an outer member protein involved in the attachment and invasion of m cells. invasin is encoded by inv, which is positively regulated by rova in both y. enterocolitica and yersinia pseudotuberculosis while negatively regulated by ymoa in y. enterocolitica and h-ns in y. pseudotuberculosis. in this study we present data indicating h-ns and rova bind directly and specifically to the inv promoter of y. ent ... | 2006 | 16816182 |
| modulation of complement activity in vitro and in vivo by yersinia wild and mutant strains. | the ability of released proteins (yops) and surface lipopolysaccharides (lps) from the wild-type strain yersinia enterocolitica 8081-l2, serotype 0:8 to influence the complement activity was determined. yops and lps from wild-type and mutant strains showed different ability to affect the classical pathway (cp) functional complement activity in vitro. the serum cp activity was inhibited during the infection induced with six y. enterocolitica and three y. pseudotuberculosis strains in rabbits. the ... | 2006 | 16821708 |
| a computational tool for the genomic identification of regions of unusual compositional properties and its utilization in the detection of horizontally transferred sequences. | similarity plot (s-plot) is a windows-based application for large-scale comparisons and 2-dimensional visualization of compositional similarities between genomic sequences. this application combines 2 approaches widely used in genomics: window analysis of statistical characteristics along genomes and dot-plot visual representation. s-plot is effective in identifying highly similar regions between genomes as well as regions with unusual compositional properties (rucps) within a single genome, whi ... | 2006 | 16829541 |
| [adaptive ultrastructural changes in soil-resident yersinia pseudotuberculosis bacteria]. | after the prolonged residence (from 1 month to 2 years) in flow soil columns at 6 - 8 degrees c and 18 - 20 degrees c a complex of ultrastructural changes was detected in y. pseudotuberculosis bacteria, depending on temperature, the duration of residence in the soil and, to a definite extent, on the strain. they were manifested in the form of cell-wall changes, the formation of the capsule and intercellular slime, changes in the ribosomal saturation of cytoplasm and the conformation state of dna ... | 2006 | 16830587 |
| [immunogenicity of b-antigen in experimental plague and pseudotuberculosis]. | the results of the evaluation of the immunogenic properties of b-antigen, earlier identified in the culture fluid of yersinia pseudotuberculosis submerged culture, with respect to experimental plague and pseudotuberculosis are presented. b-antigen has been shown to produce protective effect in guinea pigs and, probably, hamadryas baboons, but not in white mice infected with the causative agent of plague. immunizaton with b-antigen protects guinea pigs from primary pneumonic plague caused by both ... | 2006 | 16830590 |
| [yersinia lipopolysaccharide and its biological activity]. | the data on the structure and biological activity of the lipopolysaccharide (lps) of yersinia as an important virulence factor are analyzed. the biological effects of lps are characterized by dose dependence: small doses stimulate the intensity of phagocytosis, while large doses decrease phagocytic activity and produce cytotoxic effect. yersinia lps plays an important role in the development of such consequences of yersiniosis as reactive arthritis, erythema nodosum, reiter's syndrome. yersinia ... | 2006 | 16830602 |
| genomic comparison of yersinia pestis and yersinia pseudotuberculosis by combination of suppression subtractive hybridization and dna microarray. | in order to further figure out the genetic differences between yersinia pestis and yersinia pseudotuberculosis, and to provide novel insights into the evolution of y. pestis, we compared the genomes of y. pseudotuberculosis serogroup i strain atcc29833 and y. pestis antiqua strain 49006 using a combination of suppression subtractive hybridization (ssh) and comparative genomic hybridization with dnas from a diverse panel of y. pestis and y. pseudotuberculosis strains. ssh followed by blast analys ... | 2006 | 16832628 |
| genome evolution and functional divergence in yersinia. | the steadily increasing number of prokaryotic genomes has accelerated the study of genome evolution; in particular, the availability of sets of genomes from closely related bacteria has made exploration of questions surrounding the evolution of pathogenesis tractable. here we present the results of a detailed comparison of the genomes of yersinia pseudotuberculosis ip32593 and three strains of yersinia pestis (co92, kim10, and 91001). there appear to be between 241 and 275 multigene families in ... | 2007 | 16838303 |
| the serodiagnosis of human infections with yersinia enterocolitica and yersinia pseudotuberculosis. | the techniques of sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and immunoblotting were evaluated for the serodiagnosis of human infections with yersinia enterocolitica and yersinia pseudotuberculosis. lipopolysaccharide (lps) was prepared from strains comprising four serogroups of y. enterocolitica and five serogroups of y. pseudotuberculosis, tested against 200 sera submitted to the laboratory of enteric pathogens for routine serodiagnosis, and shown to contain antibodie ... | 2006 | 16872375 |
| yersinia pseudotuberculosis adhesins regulate tissue-specific colonization and immune cell localization in a mouse model of systemic infection. | yersinia pseudotuberculosis mutants deficient for the adhesins invasin and/or yada were injected intravenously into balb/c mice. invasin expression inhibited colonization of the liver and spleen. yada decreased liver colonization but promoted growth within the lung. the persistence of leukocytes within liver microabscesses correlated with enhanced colonization and lack of adhesin expression. | 2006 | 16920785 |
| comparison of yope and yopt activities in counteracting host signalling responses to yersinia pseudotuberculosis infection. | pathogenic yersinia species share a type iii secretion system that translocates yop effector proteins into host cells to counteract signalling responses during infection. two of these effectors, yope and yopt, downregulate rho gtpases by different mechanisms. here, we investigate whether yopt and yope are functionally redundant by dissecting the contribution of these two effectors to the pathogenesis of yersinia pseudotuberculosis in a mouse infection and tissue culture model. four days after or ... | 2006 | 16922868 |
| yersinia pestis yopj suppresses tumor necrosis factor alpha induction and contributes to apoptosis of immune cells in the lymph node but is not required for virulence in a rat model of bubonic plague. | the virulence of the pathogenic yersinia species depends on a plasmid-encoded type iii secretion system that transfers six yop effector proteins into host cells. one of these proteins, yopj, has been shown to disrupt host cell signaling pathways involved in proinflammatory cytokine production and to induce macrophage apoptosis in vitro. yopj-dependent apoptosis in mesenteric lymph nodes has also been demonstrated in a mouse model of yersinia pseudotuberculosis infection. these results suggest th ... | 2006 | 16926404 |
| rova, a global regulator of yersinia pestis, specifically required for bubonic plague. | the pathogenic species of yersinia contain the transcriptional regulator rova. in yersinia pseudotuberculosis and yersinia enterocolitica, rova regulates expression of the invasion factor invasin (inv), which mediates translocation across the intestinal epithelium. a y. enterocolitica rova mutant has a significant decrease in virulence by ld(50) analysis and an altered rate of dissemination compared with either wild type or an inv mutant, suggesting that rova regulates multiple virulence factors ... | 2006 | 16938880 |
| abdominal aortic aneurysm infected by yersinia pseudotuberculosis. | infected aneurysms caused by yersinia are very uncommon and are principally due to yersinia enterocolitica. we describe the first case of an infected aneurysm caused by yersinia pseudotuberculosis in an elderly patient with a history of atherosclerotic cardiovascular disease. | 2006 | 16954300 |
| yersinia virulence depends on mimicry of host rho-family nucleotide dissociation inhibitors. | yersinia spp. cause gastroenteritis and the plague, representing historically devastating pathogens that are currently an important biodefense and antibiotic resistance concern. a critical virulence determinant is the yersinia protein kinase a, or ypka, a multidomain protein that disrupts the eukaryotic actin cytoskeleton. here we solve the crystal structure of a ypka-rac1 complex and find that ypka possesses a rac1 binding domain that mimics host guanidine nucleotide dissociation inhibitors (gd ... | 2006 | 16959567 |
| comprehensive profiling of n-acylhomoserine lactones produced by yersinia pseudotuberculosis using liquid chromatography coupled to hybrid quadrupole-linear ion trap mass spectrometry. | a method for the comprehensive profiling of the n-acylhomoserine lactone (ahl) family of bacterial quorum-sensing molecules is presented using liquid chromatography (lc) coupled to hybrid quadrupole-linear ion trap (qqqlit) mass spectrometry. information-dependent acquisition (ida), using triggered combinations of triple-quadrupole and linear ion trap modes in the same lc-ms/ms run, was used to simultaneously screen, quantify and identify multiple ahls in a single sample. this ms method uses com ... | 2007 | 16967185 |
| a small-molecule inhibitor of type iii secretion inhibits different stages of the infectious cycle of chlamydia trachomatis. | the intracellular pathogen chlamydia trachomatis possesses a type iii secretion (tts) system believed to deliver a series of effector proteins into the inclusion membrane (inc-proteins) as well as into the host cytosol with perceived consequences for the pathogenicity of this common venereal pathogen. recently, small molecules were shown to block the tts system of yersinia pseudotuberculosis. here, we show that one of these compounds, inp0400, inhibits intracellular replication and infectivity o ... | 2006 | 16973741 |
| the use of bacterial minicells to transfer plasmid dna to eukaryotic cells. | the delivery of dna to mammalian cells is of critical importance to the development of genetic vaccines, gene replacement therapies and gene silencing. for these applications, targeting, effective dna transfer and vector safety are the major roadblocks in furthering development. in this report, we present a novel dna delivery vehicle that makes use of protoplasted, achromosomal bacterial minicells. transfer of plasmid dna as measured by green fluorescent protein expression was found to occur in ... | 2006 | 16984417 |
| the differential expression of yersinia pseudotuberculosis adhesins determines the requirement for fak and/or pyk2 during bacterial phagocytosis by macrophages. | phagocytosis of yersinia pseudotuberculosis by macrophages is initiated by interactions between host cell integrin receptors and the bacterial adhesins, invasin and yada. two non-receptor protein tyrosine kinases, fak and pyk2, have been implicated in this process. in this study, we investigated the mechanisms of activation and functional requirements for these kinases during phagocytosis. a panel of yersinia strains that differentially express invasin and yada were used to infect cells in which ... | 2007 | 16987330 |
| current trends in plague research: from genomics to virulence. | yersinia pestis is the causative agent of plague, which diverged from yersinia pseudotuberculosis within the past 20,000 years. although these two species share a high degree of homology at the dna level (>90%), they differ radically in their pathogenicity and transmission. in this review, we briefly outline the known virulence factors that differentiate these two species and emphasize genetic studies that have been conducted comparing y. pestis and y. pseudotuberculosis. these comparisons have ... | 2006 | 16988099 |
| hepatic yersiniosis in a cougar (felis concolor). | a cougar (felis concolor) was diagnosed with hepatic yersiniosis by bacterial culture and histopathology. the animal had a 2-week history of anorexia and jaundice before its death. grossly, the liver exhibited caseo-necrotic foci. histopathologically, there was necrotizing and suppurative hepatitis, with large numbers of intralesional gram-negative coccobacilli. additional hepatic lesions included central vein thrombosis, lymphoplasmacytic portal hepatitis, and capsulitis. yersinia pseudotubercu ... | 2006 | 17037628 |
| an outbreak of gastrointestinal illness and erythema nodosum from grated carrots contaminated with yersinia pseudotuberculosis. | outbreaks of yersinia pseudotuberculosis infection have been epidemiologically linked to fresh produce, but the bacterium has not been recovered from the food items implicated. in may 2003, a cluster of gastrointestinal illness and erythema nodosum was detected among schoolchildren who had eaten lunches prepared by the same institutional kitchen. | 2006 | 17041846 |
| mutations in the yersinia pseudotuberculosis type iii secretion system needle protein, yscf, that specifically abrogate effector translocation into host cells. | the trafficking of effectors, termed yops, from yersinia spp. into host cells is a multistep process that requires the type iii secretion system (ttss). the ttss has three main structural parts: a base, a needle, and a translocon, which work together to ensure the polarized movement of yops directly from the bacterial cytosol into the host cell cytosol. to understand the interactions that take place at the interface between the tip of the ttss needle and the translocon, we developed a screen to ... | 2007 | 17071752 |
| rovm, a novel lysr-type regulator of the virulence activator gene rova, controls cell invasion, virulence and motility of yersinia pseudotuberculosis. | rova is a marr-type transcriptional regulator that controls transcription of rova, the expression of the primary invasive factor invasin and other virulence genes of yersinia pseudotuberculosis in response to environmental signals. using a genetic approach to identify regulatory components that negatively influence rova expression, we identified a new lysr-type regulatory protein, designated rovm, which exhibits homology to the virulence regulator pect/hexa of plant pathogenic erwinia species. d ... | 2006 | 17074075 |
| disruption of rhogdi and rhoa regulation by a rac1 specificity switch mutant. | rho family gtpases are important regulators of the actin cytoskeleton. activation of these proteins can be promoted by guanine nucleotide exchange factors containing dbl and pleckstrin homology domains resulting in membrane insertion of a rho family member, whereas the inactive gdp-bound form is sequestered primarily in the cytoplasm, bound to the guanosine dissociation inhibitor rhogdi. dominant interfering variants of rac1, but not cdc42, inhibit beta1 integrin-promoted uptake of yersinia pseu ... | 2006 | 17074770 |
| intranasal inoculation of mice with yersinia pseudotuberculosis causes a lethal lung infection that is dependent on yersinia outer proteins and phop. | yersinia pseudotuberculosis infects many mammals and birds including humans, livestock, and wild rodents and can be recovered from the lungs of infected animals. to determine the y. pseudotuberculosis factors important for growth during lung infection, we developed an intranasal model of infection in mice. following intranasal inoculation, we monitored both bacterial growth in lungs and dissemination to systemic tissues. intranasal inoculation with as few as 18 cfu of y. pseudotuberculosis cause ... | 2007 | 17074849 |
| characterization of spnq from the spinosyn biosynthetic pathway of saccharopolyspora spinosa: mechanistic and evolutionary implications for c-3 deoxygenation in deoxysugar biosynthesis. | the c-3 deoxygenation step in the biosynthesis of d-forosamine (4-n,n-dimethylamino-2,3,4,6-tetradeoxy-d-threo-hexopyranose), a constituent of spinosyn produced by saccharopolyspora spinosa, was investigated. the spnq gene, proposed to encode a tdp-4-keto-2,6-dideoxy-d-glucose 3-dehydratase was cloned and overexpressed in e. coli. characterization of the purified enzyme established that it is a pmp and iron-sulfur containing enzyme which catalyzes the c-3 deoxygenation in a reductase-dependent m ... | 2006 | 17076492 |
| analysis of kawasaki disease showing elevated antibody titres of yersinia pseudotuberculosis. | to elucidate a clinical difference between patients with kawasaki disease documented with yersinia pseudotuberculosis infection and patients with kawasaki disease without yersinia pseudotuberculosis infection. | 2006 | 17129979 |
| amplification of sense-stranded prokaryotic rna. | microarray expression analysis has proven to be a valuable methodology. in eukaryotic systems where rna is limiting, established protocols for amplification of mrna, which rely on the poly(a) tails, are well established. in contrast, the difficulty in amplifying prokaryotic mrna has limited the application of microarrays to microbiology. here we present a method for the linear amplification of prokaryotic transcripts (lapt) that is efficient and unbiased. the overhang tailing activity of moloney ... | 2006 | 17132093 |
| isolation and characterization of a low-molecular-weight immunoglobulin-binding protein from yersinia pseudotuberculosis. | a low-molecular-weight immunoglobulin-binding protein (ibp) bound with the cell envelope has been isolated from yersinia pseudotuberculosis cells and partially characterized. this ibp is a hydrophilic protein with a high polarity index of 55.3%. the molecular weight of the protein has been determined by maldi-tof mass spectrometry as 14.3 kd. cd spectroscopy showed that the ibp has high contents of the beta-structure and random coil structure. the ibp contains glycine as the n-terminal amino aci ... | 2006 | 17140390 |
| influence of culture conditions and virulence plasmids on expression of immunoglobulin-binding proteins of yersinia pseudotuberculosis. | the influence of culture conditions and plasmids on immunoglobulin (ig)-binding activity of two isogenic strains of yersinia pseudotuberculosis (plasmid-free strain 48(-)82(-) and strain 48(+)82(+) bearing plasmids pyv48 and pvm82) was studied. the highest activity was observed in the bacteria grown on glucose-containing liquid medium in the stationary growth phase. the ig-binding activity of the bacteria cultured on the liquid medium at ph 6.0 was about 1.5-fold higher than that of the bacteria ... | 2006 | 17140391 |
| [genotyping of yersinia pseudotuberculosis isolated in siberia and far east]. | genotypic characteristics based three main factors of pathogenicity (presence of resident plasmids [pyv, pvm], gene of toxin-superantigen ypm and nine genes for high pathogenicity island [hpi]) of 212 strains of y. pseudotuberculosis isolated in siberia and far east were studied. it was shown that strains of y. pseudotuberculosis with one of two variants of plasmids 82:47 mda and 47 mda (60.8% and 31.6% respectively) are predominated. gene ypma was detected in 96.2% of isolated strains. eight st ... | 2006 | 17163133 |
| [yersinia pseudotuberculosis toxins]. | the review of publications about protein toxins y. pseudotuberculosis are presented. it includes the main data obtained by domestic and foreign investigators as well as the results of our own elaboration in the study of y. pseudotuberculosis protein toxins. the guestions of isolation, purification, characterization of physico-chemical and biological properties, the mechanism action and role of toxins on pathogenesis of infection were discussed. | 2006 | 17163146 |
| the complete genome sequence and comparative genome analysis of the high pathogenicity yersinia enterocolitica strain 8081. | the human enteropathogen, yersinia enterocolitica, is a significant link in the range of yersinia pathologies extending from mild gastroenteritis to bubonic plague. comparison at the genomic level is a key step in our understanding of the genetic basis for this pathogenicity spectrum. here we report the genome of y. enterocolitica strain 8081 (serotype 0:8; biotype 1b) and extensive microarray data relating to the genetic diversity of the y. enterocolitica species. our analysis reveals that the ... | 2006 | 17173484 |
| oral inoculation with type iii secretion mutants of yersinia pseudotuberculosis provides protection from oral, intraperitoneal, or intranasal challenge with virulent yersinia. | the enteric pathogen yersinia pseudotuberculosis (yptb) causes gastroenteritis, mesenteric lymphadenitis, and systemic infections in humans, livestock, and wild animals. yptb type iii secretion system (pttss) mutants efficiently colonize lymphoid tissues, but not the gastrointestinal tract, spleen, or liver. here, we show that a single oral inoculation of pttss mutants prevents morbidity in almost 100% of mice challenged intragastrically with virulent yptb. in addition, a single oral inoculation ... | 2007 | 17194509 |
| yersinia pseudotuberculosis infection of a lumbar facet joint. | 2007 | 17197222 | |
| minimal yopb and yopd translocator secretion by yersinia is sufficient for yop-effector delivery into target cells. | pathogenic yersinia sp. utilise a common type iii secretion system to translocate several anti-host yop effectors into the cytosol of target eukaryotic cells. the secreted yopb and yopd translocator proteins are essential for this process, forming pores in biological membranes through which the effectors are thought to gain access to the cell interior. the non-secreted cognate chaperone, lcrh, also plays an important role by ensuring pre-secretory stabilisation and efficient secretion of yopb an ... | 2007 | 17223369 |
| a horizontally acquired filamentous phage contributes to the pathogenicity of the plague bacillus. | yersinia pestis, the plague bacillus, has an exceptional pathogenicity but the factors responsible for its extreme virulence are still unknown. a genome comparison with its less virulent ancestor yersinia pseudotuberculosis identified a few y. pestis-specific regions acquired after their divergence. one of them potentially encodes a prophage (ypfphi), similar to filamentous phages associated with virulence in other pathogens. we show here that ypfphi forms filamentous phage particles infectious ... | 2007 | 17238929 |
| small molecule inhibitors of type iii secretion in yersinia block the chlamydia pneumoniae infection cycle. | intracellular parasitism by chlamydiales is a complex process involving transmission of metabolically inactive particles that differentiate, replicate, and re-differentiate within the host cell. a type three secretion system (t3ss) has been implicated in this process. we have here identified small molecules of a chemical class of acylated hydrazones of salicylaldehydes that specifically blocks the t3ss of chlamydia. these compounds also affect the developmental cycle showing that the t3ss has a ... | 2007 | 17257594 |
| cutaneous infection with yersinia pseudotuberculosis presenting with sporotrichoid spread. | 2007 | 17288867 | |
| change in substrate specificity of cytotoxic necrotizing factor unmasks proteasome-independent down-regulation of constitutively active rhoa. | cytotoxic necrotizing factors cnf1 and cnf2 are produced by pathogenic escherichia coli strains. they constitutively activate small gtpases of the rho family by deamidation of a glutamine, which is crucial for gtp hydrolysis. recently, a novel cnf (cnf(y)) encompassing 65% identity to cnf1 has been identified in yersinia pseudotuberculosis. in contrast to the e. coli toxins, which activate several isoforms of rho family gtpases, cnf(y) is a strong and selective activator of rhoa in vivo. by cons ... | 2007 | 17296609 |
| a north american yersinia pestis draft genome sequence: snps and phylogenetic analysis. | yersinia pestis, the causative agent of plague, is responsible for some of the greatest epidemic scourges of mankind. it is widespread in the western united states, although it has only been present there for just over 100 years. as a result, there has been very little time for diversity to accumulate in this region. much of the diversity that has been detected among north american isolates is at loci that mutate too quickly to accurately reconstruct large-scale phylogenetic patterns. slowly-evo ... | 2007 | 17311096 |
| a retro-evolution study of cdp-6-deoxy-d-glycero-l-threo-4-hexulose-3-dehydrase (e1) from yersinia pseudotuberculosis: implications for c-3 deoxygenation in the biosynthesis of 3,6-dideoxyhexoses. | cdp-6-deoxy-l-threo-d-glycero-4-hexulose-3-dehydrase (e1), which catalyzes c-3 deoxygenation of cdp-4-keto-6-deoxyglucose in the biosynthesis of 3,6-dideoxyhexoses, shares a modest sequence identity with other b6-dependent enzymes, albeit with two important distinctions. it is a rare example of a b6-dependent enzyme that harbors a [2fe-2s] cluster, and a highly conserved lysine that serves as an anchor for plp in most b6-dependent enzymes is replaced by histidine at position 220 in e1. since alt ... | 2007 | 17323931 |
| yersinia pseudotuberculosis infection in a suckler calf in the united kingdom: a case report. | enterocolitis caused by yersinia pseudotuberculosis was identified by bacterial culture and histopathology in a 5-month old suckler calf. no other significant enteric pathogens were detected. diagnosis of this condition can be missed if selective media are not used for bacteriology and if histopathology is not attempted or the alimentary tract is unsuitable for examination. this is the first case report of y. pseudotuberculosis infection in a calf in the united kingdom. | 2008 | 17329133 |
| yersinia pseudotuberculosis superantigens. | yersinia pseudotuberculosis, a gastro-intestinal bacterium, produces three closely related t cell superantigens, ypma, ypmb and ypmc, which have no significant sequence similarity to other proteins, let alone other bacterial superantigens. y. pseudotuberculosisderived mitogen (ypm) has been shown to play a role in the pathogenesis of human and animal y. pseudotuberculosis infection. the three-dimensional structure of ypma, as determined by x-ray crystallography and nuclear magnetic resonance spe ... | 2007 | 17369701 |
| modulation of innate and acquired immunity by an estrogenic dose of genistein in gonadectomized cats. | dietary genistein has potential as a surrogate estrogen to restore normal control of food intake in cats following gonadectomy. however, since genistein affects immunological responses in other species it is important to determine if there is any immunological effect of genistein in cats, before long-term feeding of it could be considered. it was hypothesized that the minimum estrogenic dose of genistein (peak serum concentration 0.276+/-0.055 microg/ml) would be associated with altered innate a ... | 2007 | 17382408 |
| plague. | bubonic plague is an often fulminant systemic zoonosis, caused by yersinia pestis. conventional microbiology, bacterial population genetics, and genome sequence data, all suggest that y pestis is a recently evolved clone of the enteric pathogen yersinia pseudotuberculosis. the genetic basis of this organism's rapid adaptation to its insect vector (the flea) with transmission between mammalian hosts by novel subcutaneous and pneumonic routes of infection is becoming clearer. this transition provi ... | 2007 | 17416264 |
| growth of yersinia pseudotuberculosis in mice occurs independently of toll-like receptor 2 expression and induction of interleukin-10. | pathogenic yersinia translocates effector proteins into target cells via a type iii secretion system (ttss), modulating the host immune response. a component of the ttss translocon, lcrv, has been implicated in preventing inflammation through toll-like receptor 2 (tlr2) by inducing expression of the anti-inflammatory cytokine interleukin-10 (il-10). tlr2(-/-) mice were reported to be less susceptible to the enteropathogen yersinia enterocolitica. to determine whether tlr2 also plays a role in re ... | 2007 | 17420232 |
| relationships between plants and enterobacteria that are pathogenic for humans. | 2006 | 17425047 | |
| the cytotoxic necrotizing factors from yersinia pseudotuberculosis and from escherichia coli bind to different cellular receptors but take the same route to the cytosol. | the cytotoxic necrotizing factors cnf1 and cnf2 produced by pathogenic escherichia coli strains and cnf(y) of yersinia pseudotuberculosis constitutively activate small gtpases of the rho family. they deamidate a glutamine (gln63 in rhoa), which is crucial for gtp hydrolysis. cnf1 and cnf(y) exhibit 61% identity on the amino acid level, with equal distribution over the whole molecule. although the two toxins are homologous in the receptor binding domain, we show that they bind to different cellul ... | 2007 | 17438028 |
| phenotypic characterization of a virulence-associated protein, vagh, of yersinia pseudotuberculosis reveals a tight link between vagh and the type iii secretion system. | recently, a number of attenuated mutants of yersinia pseudotuberculosis have been identified using a bioinformatics approach. one of the target genes identified in that study was vagh, which the authors now characterized further. vagh shows homology to hemk of escherichia coli, possessing methyltransferase activity similar to that of hemk, and targeting release factors 1 and 2. microarray studies comparing the wild-type and the vagh mutant revealed that the mrna levels of only a few genes were a ... | 2007 | 17464060 |
| extracytoplasmic-stress-responsive pathways modulate type iii secretion in yersinia pseudotuberculosis. | three signal transduction pathways, the two-component systems cpxra and baesr and the alternative sigma factor sigma(e), respond to extracytoplasmic stress that facilitates bacterial adaptation to changing environments. at least the cpxra and sigma(e) pathways control the production of protein-folding and degradation factors that counter the effects of protein misfolding in the periplasm. this function also influences the biogenesis of multicomponent extracellular appendages that span the bacter ... | 2007 | 17517869 |
| application of multiplex pcr for monitoring colonization of pig tonsils by yersinia enterocolitica, including biotype 1a, and yersinia pseudotuberculosis. | a multiplex pcr assay was developed for the detection and differentiation of the yersinia enterocolitica and yersinia pseudotuberculosis isolates in both pure bacterial cultures and pig tonsils. the assay was based on the amplification of the ail, inv, yada, and ystb genes. the pcr products, corresponding to the ail gene and the plasmid-borne yada gene or only one product corresponding to the ail gene, were detected in y. enterocolitica 4 biotype isolates. all of the y. pseudotuberculosis isolat ... | 2007 | 17536668 |
| the weak interaction of lcrv and tlr2 does not contribute to the virulence of yersinia pestis. | yersinia pestis and the enteropathogenic yersinia pseudotuberculosis and yersinia enterocolitica share the virulence-antigen lcrv. previously, using reverse genetics we have proven that lcrv contributes to the virulence of y. enterocolitica serotype o:8 by inducing il-10 via toll-like receptor 2 (tlr2). however, both the ability of y. pestis lcrv to activate tlr2 and a possible role of tlr2-dependent il-10 induction by lcrv in y. pestis are not yet known. to eliminate interference from additiona ... | 2007 | 17556003 |
| expression and insecticidal activity of yersinia pseudotuberculosis and photorhabdus luminescens toxin complex proteins. | photorhabdus luminescens toxin complex (tc) has been characterized as a potent three-component insecticidal protein complex. homologues of genes encoding p. luminescens tc components have been identified in several other enterobacteria and in gram-positive bacteria, showing these genes are widespread in bacteria. in particular, tc gene homologues have been identified in yersinia enterocolitica, yersinia pseudotuberculosis and yersinia pestis and may have a role in y. pestis evolution. y. enteroc ... | 2007 | 17573906 |
| acute oral toxicity of yersinia pseudotuberculosis to fleas: implications for the evolution of vector-borne transmission of plague. | yersinia pestis diverged from yersinia pseudotuberculosis</= 20 000 years ago, during which time it evolved to be transmitted by fleas. in comparing the ability of these closely related species to infect the rat flea xenopsylla cheopis, we found that y. pseudotuberculosis, unlike y. pestis, is orally toxic to fleas. fleas showed signs of acute toxicity, including diarrhoea, immediately after feeding on blood containing y. pseudotuberculosis in response to protein toxin(s) produced by the bacteri ... | 2007 | 17587333 |
| similarities of kawasaki disease and yersinia pseudotuberculosis infection epidemiology. | although an infectious etiology is strongly suspected in kawasaki disease (kd), an etiologic agent has not yet been identified. by reviewing epidemiologic data published in past decades, this work highlights a higher incidence of kd when populations were exposed to the risk of yersinia pseudotuberculosis infection. this hitherto unnoticed element reinforces the hypothesis whereby this bacterium might contribute to the development of kd in some cases. | 2007 | 17596806 |
| regulation of yersinia yop-effector delivery by translocated yope. | the bacterial pathogen yersinia pseudotuberculosis uses a type iii secretion (t3s) system to translocate yop effectors into eukaryotic cells. effectors are thought to gain access to the cytosol via pores formed in the host cell plasma membrane. translocated yope can modulate this pore formation through its gtpase-activating protein (gap) activity. in this study, we analysed the role of translocated yope and all the other known yop effectors in the regulation of effector translocation. elevated l ... | 2008 | 17597003 |
| analysis of the aerobactin and ferric hydroxamate uptake systems of yersinia pestis. | yersinia pestis genomes contain genes homologous to the aerobactin receptor (iuta) and biosynthetic genes (iucabcd) as well as the ferric hydroxamate uptake system (fhucdb) of escherichia coli. however, iuca is disrupted by a frameshift mutation. an e. coli strain carrying the cloned y. pestis aerobactin region was unable to produce aerobactin, but could use the siderophore as an iron source. repair of the frameshift mutation in iuca did not allow aerobactin production in e. coli or y. pestis. i ... | 2007 | 17600077 |
| influence of the cpx extracytoplasmic-stress-responsive pathway on yersinia sp.-eukaryotic cell contact. | the extracytoplasmic-stress-responsive cpxra two-component signal transduction pathway allows bacteria to adapt to growth in extreme environments. it controls the production of periplasmic protein folding and degradation factors, which aids in the biogenesis of multicomponent virulence determinants that span the bacterial envelope. this is true of the yersinia pseudotuberculosis ysc-yop type iii secretion system. however, despite using a second-site suppressor mutation to restore yop effector se ... | 2007 | 17620356 |
| [thermotropic behavior of lipids and the morphology of yersinia pseudotuberculosis cells with a high content of lysophosphatidylethanolamine]. | the content of lysophosphatidylethanolamine (lpe) in y. pseudotuberculosis cells was found to increase during their growth at 8 degrees c under stationary conditions (without stirring the medium) and at 37 degrees c when the medium contained glucose. the maximum level of lpe (up to 45% of the total phospholipids) was observed in cells grown at 8 degrees c under stationary conditions. such cells showed an enhanced growth rate, a reduced yield of biomass, an altered cell morphology, and an increas ... | 2007 | 17633407 |
| [humoral response to selected antigens of yersinia enterocolitica and yersinia pseudotuberculosis in the course of yersiniosis in humans. i. occurrence of antibodies to yersinia lipopolisacharydes and yop proteins by elisa]. | the antibodies against the somatic antigens of y. enterocolitica o3, o8, o9, o5,27,y. pseudotuberculosis i, and released proteins yop were detected using the elisa in 1634 serum samples and 84 synovial fluids collected from 1290 persons suspected for yersiniosis, as well as 200 serum samples from healthy individuals (blood donors). the presence of antibody in diagnostically significant titres for somatic antigens of yersinia were detected by elisa in 20.5% and 50.6%, antibodies for released prot ... | 2006 | 17642308 |
| [humoral response to selected antigens of yersinia enterocolitica and yersinia pseudotuberculosis in the course of yersiniosis in humans. i. occurrence of antibodies to yersinia yop proteins by western-blot]. | the results obtained with the use of the western-blotting showed that antibodies for released proteins yopd (33-36 kda) were the most frequently detected antibodies in serum samples from patients suspected for yersiniosis. reactions between serum samples studied and the yopd protein were very intense, suggesting that protein is the strongest immunogen among the utilised, released proteins yop of yersinia. antibodies igm were more often diagnosed in patients with abdominal pain in the contrary to ... | 2006 | 17642309 |
| a putative dna adenine methyltransferase is involved in yersinia pseudotuberculosis pathogenicity. | some adenine methyltransferases have been shown not only to protect specific dna restriction sites from cleavage by a restriction endonuclease, but also to play a role in various bacterial processes and sometimes in bacterial virulence. this study focused on a type i restriction-modification system (designated yrmi) of y. pseudotuberculosis. this system is composed of three adjacent genes which could potentially encode an n6-adenine dna methylase (yama), an enzyme involved in site-specific recog ... | 2007 | 17660407 |
| [laboratory diagnosis of yersinia infection and its improvement]. | results of the bacteriological and serological tests of patients with y. enterocolitica and y. pseudotuberculosis infections for the period from 1994 to 2004 were analyzed. main reasons of imperfect laboratory diagnostics were revealed, such as, low sensitivity of bacteriologic test, nonobservance of existing recommendations on diagnostics of yersinia infections, performing of single but not repeated serologic test, absence of necessary laboratory equipment. main ways of improving of quality of ... | 2007 | 17672133 |
| [influence of chemical and physical characteristics of seashore soils in primorsky region on preservation and reproduction of listeria and yersinia]. | influence of chemical and physical characteristics of two types of seashore soils (tidal marsh and maritime meadow soils) in primorsky region on reproduction of listeria monocytogenes and yersinia pseudotuberculosis was studied. results of experiments proved the possibility of preservation and active reproduction of these bacteria in studied soils. characteristics of maritime meadow soil are more favorable for mentioned microorganisms than tidal march soil that located closer to the sea. | 2007 | 17672139 |
| prevention and treatment of colitis with lactococcus lactis secreting the immunomodulatory yersinia lcrv protein. | the low calcium response v (lcrv) protein synthesized by gram-negative, pathogenic yersiniae participates in bacterial evasion of the host's innate immune response by stimulating synthesis of the anti-inflammatory interleukin (il)-10 and preventing the activation of proinflammatory cytokines. | 2007 | 17678918 |
| [new accumulation medium for isolation of bacteria of the genus yersinia]. | liquid nutrient peptone-potassium accumulation medium (ppam) has been designed to isolate yersinia when samples taken from patients, animals, and environmental objects are examined. ppam contains enzymatic peptone and disubstituted potassium phosphate. ppam ensures a rapid yersinia and accordingly early (days 2-3) isolation of at least 23.4% of yersinia cultures, suppresses the growth of the associating flora (under the conditions of model experiments by 2-3 orders against one on the buffer-case ... | 2007 | 17682482 |
| how the structural gene products of yersinia pestis relate to virulence. | bubonic plague is the most devastating acute infectious disease known to man. the causative agent, yersinia pestis, is now more firmly entrenched in sylvatic reservoirs throughout the world than at any time in the past. consequently, the organism increasingly causes casual human disease and is readily available for use as a bioweapon. recent attempts to understand the severe nature of plague have focused upon its very recent divergence from yersinia pseudotuberculosis, an etiological instrument ... | 2007 | 17683274 |
| prediction of antigenically active regions in the ompf-like porin of yersinia pseudotuberculosis. | 2007 | 17695318 | |
| ribotyping and virulence markers of yersinia pseudotuberculosis strains isolated from animals in brazil. | ribotyping and virulence markers has been used to investigate 68 yersinia pseudotuberculosis strains of serogroups o:1a and o:3. the strains were isolated from clinical material obtained from healthy and sick animals in the southern region of brazil. ribotypes were identified by double digestion of extracted dna with the restriction endonucleases smai and psti, separation by electrophoresis and hybridization with a digoxigenin-labeled cdna probe. the presence of the chromosomal virulence marker ... | 2007 | 17710303 |
| [elaboration of new adjuvant lipid-saponin complex and its use at experimental immunization by bacterial antigen]. | results of experiments on modification of immunostimulating complexes (iscom's) matrix by the replacement of the phospholipid for the glycolipid (monogalactosyldiacylglycerol) from sea macrophytes, and saponin quilla to triterpene glycoside of cucumarioside a2-2 from cucumaria japonica are shown. the resultant complexes include the morphological structures of two types: iscom-like structures with the characteristic morphology and sizes and also the tubular structures with diameter of approximate ... | 2007 | 17722580 |
| phenotypic analysis of yersinia pseudotuberculosis 32777 response regulator mutants: new insights into two-component system regulon plasticity in bacteria. | two-component regulatory systems (2css) typically comprise a sensor kinase and a response regulator that, in concert, monitor the concentration of particular extracellular factors and mediate the transcription of specific genes accordingly. as such, 2css play an important role in the regulation of bacterial pathogenesis. on the basis of genome-wide in silico analysis, the gram-negative enteropathogenic bacterium yersinia pseudotuberculosis is thought to encode 24 complete 2css. in the present wo ... | 2008 | 17765656 |
| phenotypic characterization of ompx, an ail homologue of yersinia pestis kim. | the goal of this study was to characterize the yersinia pestis kim ompx protein. yersinia spp. provide a model for studying several virulence processes including attachment to, and internalization by, host cells. for yersinia enterocolitica and yersinia pseudotuberculosis, ail, yada and inv, have been implicated in these processes. in y. pestis, yada and inv are inactivated. genomic analysis of two y. pestis strains revealed four loci with sequence homology to ail. one of these genes, designated ... | 2007 | 17768237 |
| the complete genome sequence of yersinia pseudotuberculosis ip31758, the causative agent of far east scarlet-like fever. | the first reported far east scarlet-like fever (feslf) epidemic swept the pacific coastal region of russia in the late 1950s. symptoms of the severe infection included erythematous skin rash and desquamation, exanthema, hyperhemic tongue, and a toxic shock syndrome. the term feslf was coined for the infection because it shares clinical presentations with scarlet fever caused by group a streptococci. the causative agent was later identified as yersinia pseudotuberculosis, although the range of mo ... | 2007 | 17784789 |
| [relationship between the level of serum rearrangement of vaccinated animals and the stress of immunity to experimental plague]. | 2007 | 17802808 | |
| pasteurella pestis: role of pesticin i and iron in experimental plague. | loss of the genetic determinant for pesticin i in pasteurella pestis results in concomitant loss of the plague coagulase and fibrinolytic factor. the median lethal dose for mice of an isolate lacking only these activities is increased by factors of about 10(1), 10(4), and 10(7) cells when administered by the intravenous, intraperitoneal, and subcutaneous routes, respectively. virulence of the aforesaid strain can be enhanced in mice treated with 40 microg of ferrous iron. this response resembles ... | 1965 | 17809405 |
| inhibitors of type iii secretion in yersinia: design, synthesis and multivariate qsar of 2-arylsulfonylamino-benzanilides. | compound 1, 2-(benzo[1,2,5]thiadiazole-4-sulfonylamino)-5-chloro-n-(3,4-dichloro-phenyl)-benzamide, was identified as a putative type iii secretion inhibitor in yersinia, and the compound thus has a potential to be used to prevent or treat bacterial infections. a set of seven analogues was synthesized and evaluated in a type iii secretion dependent reporter-gene assay with viable bacterial to give basic sar. a second set of 19 compounds was obtained by statistical molecular design in the buildin ... | 2007 | 17851084 |
| diminished lcrv secretion attenuates yersinia pseudotuberculosis virulence. | many gram-negative bacterial pathogenicity factors that function beyond the outer membrane are secreted via a contact-dependent type iii secretion system. two types of substrates are predestined for this mode of secretion, namely, antihost effectors that are translocated directly into target cells and the translocators required for targeting of the effectors across the host cell membrane. n-terminal secretion signals are important for recognition of the protein cargo by the type iii secretion ma ... | 2007 | 17873031 |
| [approaches to species-specific typing of double mixed cultures comprising pseudotuberculosis bacteria and atypical plague bacillus strains]. | the species relevance of atypical yersinia strains was determined by various microbiological, immunological, and genetic (including polymerase chain reaction) tests. these strains were shown to represent mixed cultures of y. pseudotuberculosis serovariant o1b and y. pestis var antiqua. identification-resistant cells with atypical properties and plasmid segregation were found in the populations of y. pestis strains. analysis of different diagnostic tests revealed the most reliable ones selected f ... | 2007 | 17915487 |
| dna fingerprinting analysis of breakthrough outbreaks in vaccine-protected poultry stocks. | we report recurrent outbreaks of yersinia pseudotuberculosis conjunctivitis in ducks and of fowl cholera in geese, occurring in stocks previously vaccinated with inactivated autogenous vaccines. enterobacterial repetitive intergenic consensus sequence-based pcr and pulsed-field gel electrophoresis indicated reinfection with a new y. pseudotuberculosis strain and vaccine evasion by the same pasteurella multocida strain. | 2007 | 17928428 |
| [humoral response to selected antigens of yersinia enterocolitica and yersinia pseudotuberculosis in the course of yersiniosis in humans. i. occurrence of antibodies to enterobacterial common antigen (eca)]. | the antibodies against the enterobacterial common antigen (eca) were detected using the elisa in 293 serum samples collected from 185 persons suspected for yersiniosis, as well as 115 serum samples from healthy individuals (blood donors). the presence of iga antibody in diagnostically significant titres for eca were detected by elisa in 3.5%, igg in 13.0%, and igm in 5.2% of blood donors. statistical analysis showed that the frequency of detecting antibodies for eca among the patients with yersi ... | 2007 | 17929407 |
| tentacle probes: differentiation of difficult single-nucleotide polymorphisms and deletions by presence or absence of a signal in real-time pcr. | false-positive results are a common problem in real-time pcr identification of dna sequences that differ from near neighbors by a single-nucleotide polymorphism (snp) or deletion. because of a lack of sufficient probe specificity, post-pcr analysis, such as a melting curve, is often required for mutation differentiation. | 2007 | 17932130 |
| pestoides f, an atypical yersinia pestis strain from the former soviet union. | unlike the classical yersinia pestis strains, members of an atypical group of y. pestis from central asia, denominated y. pestis subspecies caucasica (also known as one of several pestoides types), are distinguished by a number of characteristics including their ability to ferment rhamnose and melibiose, their lack of the small plasmid encoding the plasminogen activator (pla) and pesticin, and their exceptionally large variants of the virulence plasmid pmt (encoding murine toxin and capsular ant ... | 2007 | 17966401 |
| a new asset for pathogen informatics--the enteropathogen resource integration center (eric), an niaid bioinformatics resource center for biodefense and emerging/re-emerging infectious disease. | eric (enteropathogen resource information center) is one of the national institute of allergy and infectious diseases (niaid) bioinformatics resource centers for biodefense and emerging/re-emerging infectious disease. eric serves as a comprehensive information resource for five related pathogens: yersinia enterocolitica, yersinia pestis, diarrheagenic e. coli, shigella spp., and salmonella spp. eric integrates genomics, proteomics, biochemical and microbiological information to facilitate the in ... | 2007 | 17966403 |
| intermediary metabolism, na+, the low calcium-response, and acute disease. | the variables carriage of pcd, co2 tension, exogenous atp, l-glutamate, mg2+, na+, ph, source of energy, and temperature are known to modulate the low calcium response of yersinia pestis in vitro. the role of these effectors and the basis of their interactions are defined here with emphasis on known y. pestis-specific missense mutations in glucose 6-phosphate dehydrogenase and aspartase, which preclude use of the hexose monophosphate pathway and prevent efficient catabolism of l-glutamic acid, r ... | 2007 | 17966409 |
| differential gene regulation in yersinia pestis versus yersinia pseudotuberculosis: effects of hypoxia and potential role of a plasmid regulator. | the molecular basis of the biological differences between yersinia pestis and yersinia pseudotuberculosis remains largely unknown, and relatively little is known about environmental regulation of gene expression in these bacteria. we used a proteomic approach to explore the regulatory response of each bacterium to carbon dioxide-supplemented hypoxic conditions. both organisms responded similarly and the magnitude of their responses was similar to what was observed in low iron conditions. we also ... | 2007 | 17966410 |
| two-component system regulon plasticity in bacteria: a concept emerging from phenotypic analysis of yersinia pseudotuberculosis response regulator mutants. | in bacteria, the most rapid and efficient means of adapting gene transcription to extracellular stresses often involves sophisticated systems referred to as two-component systems (2css). although highly conserved throughout the bacterial world, some of these systems may control distinct cell events and have differing contributions to virulence, depending on the species considered. this chapter summarizes the work performed by our group--from the initial phop-phoq and pmra-pmrb studies to the mos ... | 2007 | 17966411 |
| regulatory elements implicated in the environmental control of invasin expression in enteropathogenic yersinia. | during infections of the intestinal tract yersinia pseudotuberculosis penetrates the epithelial cell layer through m-cells into the peyer's patches. this early step in the infection process is primarily mediated by the outer membrane protein invasin. expression of the invasin gene is activated by the marr-type regulatory protein rova in response to environmental conditions, including temperature and growth phase. in order to gain insight into the nature of the underlying control systems, mutagen ... | 2007 | 17966412 |
| the insect toxin complex of yersinia. | many members of the yersinia genus encode homologues of insect toxins first observed in bacteria that are insect pathogens such as photorhabdus, xenorhabdus and serratia entomophila. these bacteria secrete high molecular weight insecticidal toxins comprised of multiple protein subunits, termed the toxin complexes or tc's. in photorhabdus three distinct tc subunits are required for full oral toxicity in insects, that include the [a], [b] and [c] types, although the exact stochiometry remains uncl ... | 2007 | 17966421 |
| twin arginine translocation in yersinia. | bacteria utilise twin arginine translocation (tat) to deliver folded proteins across the cytoplasmic membrane. disruption of tat typically results in pleiotropic effects on e.g. growth, stress resistance, bacterial membrane biogenesis, motility and cell morphology. further, tat is coupled to virulence in a range of pathogenic bacteria, including species of pseudomonas, legionella, agrobacterium and mycobacterium. we have investigated this, for yersinia, previously unexplored system, and have sho ... | 2007 | 17966422 |
| a rationale for repression and/or loss of motility by pathogenic yersinia in the mammalian host. | pathogenic yersiniae either repress flagella expression under host conditions (yersinia enterocolitica and yersinia pseudotuberculosis) or have permanently lost this capability by mutation (yersinia pestis). the block in flagella synthesis for the enteropathogenic yersinia centers on flia (sigmaf) repression. this repression ensures the downstream repression of flagellin structural genes which can be cross-recognized and secreted by virulence type iii secretion systems. y. pestis carries several ... | 2007 | 17966426 |
| analysis of the three yersinia pestis crispr loci provides new tools for phylogenetic studies and possibly for the investigation of ancient dna. | the precise nature of the pathogen having caused early plague pandemics is uncertain. although yersinia pestis is a likely candidate for all three plague pandemics, the very rare direct evidence that can be deduced from ancient dna (adna) analysis is controversial. moreover, which of the three biovars, antiqua, medievalis or orientalis, was associated with these pandemics is still debated. there is a need for phylogenetic analysis performed on y. pestis strains isolated from countries from which ... | 2007 | 17966429 |
| high throughput screening for small-molecule inhibitors of type iii secretion in yersinia pestis. | yersinia pestis, yersinia pseudotuberculosis and yersinia enterocolitica, utilize a plasmid encoded type iii secretion system (t3ss) to promote infection by delivering yersinia outer proteins (yops) into the cytosol of mammalian cells. this t3ss is absolutely required for yersinia virulence, which makes t3ss an attractive target in the development of novel therapeutics for treatment of plague and other yersinia infections. in this study, a new method for high throughput screening (hts) of small ... | 2007 | 17966433 |
| the flhdc gene affects motility and biofilm formation in yersinia pseudotuberculosis. | the flagella master regulatory gene flhdc of yersinia pseudotuberculosis serotype iii (ypiii) was mutated by deleting the middle region and replaced by a tetracycline resistant gene, and the subsequent mutant strain named ypiiideltaflhdc was obtained. swimming assay showed that the swimming motility of the mutant strain was completely abolished. the promoter region of the flagella second-class regulatory gene flia was fused with the lux box, and was conjugated with the mutant and the parent stra ... | 2007 | 17973093 |
| design, synthesis, and multivariate quantitative structure-activity relationship of salicylanilides--potent inhibitors of type iii secretion in yersinia. | analogues to the salicylanilide n-(4-chlorophenyl)-2-acetoxy-3,5-diiodobenzamide, 1a, an inhibitor of type iii secretion (t3s) in yersinia, were selected, synthesized, and biologically evaluated in three cycles. first, a set of analogues with variations in the salicylic acid ring moiety was synthesized to probe possible structural variation. a basic structure-activity relationship was established and then used to cherry-pick compounds from a principal component analysis score plot of salicylanil ... | 2007 | 17975903 |
| characterization of integrative and conjugative element icekp1-associated genomic heterogeneity in a klebsiella pneumoniae strain isolated from a primary liver abscess. | genomic heterogeneity has been shown to be associated with klebsiella pneumoniae strains causing pyogenic liver abscesses (pla) and metastatic infections. in order to explore the mechanism responsible for genomic heterogeneity in k. pneumoniae, we compared the complete genomic sequences of strains ntuh-k2044 and mgh78578. an approximately 76-kbp dna fragment located adjacent to an asparagine (asn) trna gene was present in ntuh-k2044 but not in mgh78578. this fragment could be divided into three ... | 2008 | 17981959 |
| macrophage activation redirects yersinia-infected host cell death from apoptosis to caspase-1-dependent pyroptosis. | infection of macrophages by yersinia species results in yopj-dependent apoptosis, and naïve macrophages are highly susceptible to this form of cell death. previous studies have demonstrated that macrophages activated with lipopolysaccharide (lps) prior to infection are resistant to yopj-dependent cell death; we found this simultaneously renders macrophages susceptible to killing by yopj(-) yersinia pseudotuberculosis (yptb). yopj(-) yptb-induced macrophage death was dependent on caspase-1 activa ... | 2007 | 17983266 |
| ultraviolet inactivation kinetics of escherichia coli and yersinia pseudotuberculosis in annular reactors. | terrorist threats have precipitated the need for information on the ultraviolet (uv) resistance of potential biothreat agents in food processing, such as yersinia pestis. the objective of this study was to characterize the resistance of the yersinia species to uv treatment using a single-lamp annular uv reactor. a novel method is proposed to measure the inactivation kinetics of yersinia pseudotuberculosis, a surrogate of y. pestis. this proposed method can overcome the disadvantages of the tradi ... | 2007 | 17995726 |
| a structural study of gdp-4-keto-6-deoxy-d-mannose-3-dehydratase: caught in the act of geminal diamine formation. | di- and trideoxysugars are an important class of carbohydrates synthesized by certain plants, fungi, and bacteria. colitose, for example, is a 3,6-dideoxysugar found in the o-antigens of gram-negative bacteria such as escherichia coli, salmonella enterica, yersinia pseudotuberculosis, and vibrio cholerae, among others. these types of dideoxysugars are thought to serve as antigenic determinants and to play key roles in bacterial defense and survival. four enzymes are required for the biochemical ... | 2007 | 17997582 |