Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| molecular investigations of an outbreak of parainfluenza virus type 3 and respiratory syncytial virus infections in a hematology unit. | a large simultaneous outbreak of respiratory syncytial virus (rsv) and parainfluenza type 3 (piv-3) infections occurred on an adult hematology unit. implementation of enhanced infection control was complicated by cocirculation of the two different viruses, with prolonged viral shedding from infected patients, and placed great pressure on health care staff; of 27 infected hematopoietic stem cell transplant patients, 9 died, and the unit was closed for 2 months. retrospective molecular investigati ... | 2007 | 17392447 |
| immunization strategies for the prevention of pneumovirus infections. | pneumoviruses, which are viruses of the family paramyxoviridae, subfamily pneumovirinae, are pathogens that infect the respiratory tract of their host species. the human pneumovirus pathogen, human respiratory syncytial virus (rsv), has counterparts that infect cows (bovine rsv), sheep (ovine rsv), goats (caprine rsv) and rodents (pneumonia virus of mice). each pneumovirus is host specific and results in a spectrum of disease, ranging from mild upper-respiratory illness to severe bronchiolitis a ... | 2007 | 17408367 |
| t helper 1 background protects against airway hyperresponsiveness and inflammation in guinea pigs with persistent respiratory syncytial virus infection. | a family history of allergy has been implicated in children who develop post-bronchiolitis wheezing and asthma. in a guinea pig model of respiratory syncytial virus (rsv) lung infection, we evaluated the role of host th1 background (either genetic or induced) on the development of a persistent infection, nonspecific airway hyperresponsiveness (ahr) and airway inflammation. allergy resistant/t helper 1 (th1)-skewed strain 2 guinea pigs (str2) and cytosine phosphate guanine oligodeoxynucleotides ( ... | 2007 | 17413871 |
| lack of usefulness of an abnormal white blood cell count for predicting a concurrent serious bacterial infection in infants and young children hospitalized with respiratory syncytial virus lower respiratory tract infection. | there continues to be controversy on the most appropriate way to manage infants and young children with fever and documented rsv lower respiratory tract infection (lrti). the objective of this study was to determine the usefulness of an abnormal white blood cell (wbc) count for predicting a concurrent serious bacterial infection in patients admitted with rsv lrti. | 2007 | 17414393 |
| pertussis is common in nonvaccinated infants hospitalized for respiratory syncytial virus infection. | there is some evidence that bordetella pertussis can cause co-infection with respiratory syncytial virus (rsv). | 2007 | 17414394 |
| palivizumab prophylaxis to prevent respiratory syncytial virus mortality after pediatric bone marrow transplantation: a decision analysis model. | palivizumab, a monoclonal antibody against respiratory syncytial virus (rsv), has been demonstrated to be safe and effective in young children, but evidence is lacking as to whether palivizumab is effective in preventing rsv-induced morbidity and mortality in children who are immunosuppressed after bone marrow transplantation (bmt). as a randomized, double-blind, placebo-controlled trial is lacking, we chose to examine this issue with the use of decision analysis methodology. | 2007 | 17414564 |
| intranasal vaccination with peptides and cholera toxin subunit b as adjuvant to enhance mucosal and systemic immunity to respiratory syncytial virus. | amino acid sequences 200-225 and 255-278 of the f protein of human respiratory syncytial virus (hrsv) are t cell epitopes (bourgeois et al., 1991; corvaisier et al, 1993). peptides corresponding to these two regions were synthesized and coupled with keyhole limpet haemocyanin (klh). the two conjugated proteins were administered intranasally to balb/c mice alone or together with cholera toxin b (ctb). elisas revealed that the mixture of the conjugates with ctb increased not only the systemic resp ... | 2007 | 17424945 |
| neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (il)-4 and il-10 gene transcripts. | the lung microenvironment is constantly exposed to microorganisms and particulate matter. lung dendritic cells (dcs) play a crucial role in the uptake and processing of antigens found within the respiratory tract. respiratory syncytial virus (rsv) is a common respiratory tract pathogen in children that induces an influx of dcs to the mucosal surfaces of the lung. using a neonatal lamb model, we examined the in vivo permissiveness of dcs to rsv infection, as well as overall cell surface changes a ... | 2007 | 17425426 |
| pretreatment with recombinant human vascular endothelial growth factor reduces virus replication and inflammation in a perinatal lamb model of respiratory syncytial virus infection. | vascular endothelial growth factor (vegf) is increasingly recognized as a perinatal regulator of lung maturation and surfactant protein expression. preterm and young infants are at increased risk for pulmonary immaturity characterized by insufficient surfactant production as well as increased risk for severe manifestations of respiratory syncytial virus (rsv) infection. innate immune components including surfactant proteins a and d, and beta-defensins have putative antimicrobial activity against ... | 2007 | 17425433 |
| peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells. | respiratory syncytial virus (rsv) is the major causative agent of severe lower respiratory tract disease and death in infants worldwide. the epithelial cells of the airways are the target cells for rsv infection and the site of the majority of the inflammation associated with the disease. however, despite five decades of intensive rsv research there exist neither an effective active vaccine nor a promising antiviral and anti-inflammatory therapy. recently, peroxisome proliferator-activated recep ... | 2007 | 17425601 |
| high-throughput screening of stabilizers for respiratory syncytial virus: identification of stabilizers and their effects on the conformational thermostability of viral particles. | respiratory syncytial virus (rsv) is the leading cause of severe respiratory infection in children worldwide. recombinant live attenuated viral preparations are one of the most promising strategies for vaccination but they typically possess poor thermostability. in this work, a library of compounds was screened and stabilizers were selected based on their ability to inhibit the aggregation of rsv perturbed at 56 degrees c. after screening and selection of excipients, the conformational stability ... | 2007 | 17426457 |
| human metapneumovirus bronchiolitis in infancy is an important risk factor for asthma at age 5. | human metapneumovirus (hmpv) infection plays an important role in the pediatric respiratory infections. however, little is known about the relationship between hmpv-bronchiolitis and the development of subsequent wheezing. the aim of this study was to evaluate the outcome at third to fifth year after admission for hmpv-bronchiolitis and to compare it with children admitted for respiratory syncytial virus (rsv)-bronchiolitis and with children without lower respiratory disease in the first 2 years ... | 2007 | 17427899 |
| performance of a rapid assay (binax now) for detection of respiratory syncytial virus at a children's hospital over a 3-year period. | a rapid assay, binax now rsv, was compared to viral culture for 14,756 pediatric respiratory specimens obtained from 2003 to 2006. there were 794 viral culture-confirmed respiratory syncytial virus infections. sensitivity was 81%, and specificity was 93.2%. sensitivity was greatest for neonates (91.1% versus 80.7% [p < 0.01]). | 2007 | 17428942 |
| phenotype of acute respiratory syncytial virus induced lower respiratory tract illness in infancy and subsequent morbidity. | the objective of this study was to investigate the association hypothesis that outcome following respiratory syncytial virus (rsv) induced bronchiolitis (rsvb) and rsv induced wheeze (rsvw) are different. at 3 years respiratory symptoms were more common in those with rsv infection than the control group but there was no increase in allergic sensitisation (11% vs 10%). those with rsvw were more likely to have evidence of allergic sensitisation when compared with rsvb subjects (22% vs 7%), and hav ... | 2007 | 17429926 |
| high-efficiency promoter-dependent transduction by adeno-associated virus type 6 vectors in mouse lung. | the transduction efficiency of adeno-associated virus (aav) vectors in various somatic tissues has been shown to depend heavily on the aav type from which the vector capsid proteins are derived. among the aav types studied, aav6 efficiently transduces cells of the airway epithelium, making it a good candidate for the treatment of lung diseases such as cystic fibrosis. here we have evaluated the effects of various promoter sequences on transduction rates and gene expression levels in the lung. of ... | 2007 | 17430088 |
| respiratory syncytial virus induces insensitivity to beta-adrenergic agonists in mouse lung epithelium in vivo. | respiratory syncytial virus (rsv) is the most common cause of bronchiolitis in infants and children worldwide. we wished to determine whether intratracheal administration of beta-agonists improved alveolar fluid clearance (afc) across the distal respiratory epithelium of rsv-infected mice. following intranasal infection with rsv strain a2, afc was measured in anesthetized, ventilated balb/c mice by instillation of 5% bsa into the dependent lung. we found that direct activation of protein kinase ... | 2007 | 17435077 |
| evaluation of uplink-rsv: prototype rapid antigen test for detection of respiratory syncytial virus infection. | a prototype rapid antigen test for the on-site detection of respiratory syncytial virus (rsv) infection was developed and evaluated. the platform uses instrumented assay analysis, eliminating potential operator bias in the interpretation of the test result that may occur with visually interpreted rapid antigen assays. the device was tested as the first point-of-care (poc) infectious disease application of novel reporter up-converting phosphor technology (upt) using a specifically designed portab ... | 2007 | 17435154 |
| immunopathogenesis of respiratory syncytial virus bronchiolitis. | the objective of this study was to elucidate the relation between respiratory syncytial virus (rsv) infection and cytokine/chemokine concentrations, as well as the impact that these factors have on the severity of bronchiolitis. | 2007 | 17436234 |
| myd88-mediated instructive signals in dendritic cells regulate pulmonary immune responses during respiratory virus infection. | respiratory syncytial virus (rsv) is the leading cause of respiratory disease in infants worldwide. the induction of innate immunity and the establishment of adaptive immune responses are influenced by the recognition of pathogen-associated molecular patterns by tlrs. one of the primary pathways for tlr activation is by myd88 adapter protein signaling. the present studies indicate that myd88 deficiency profoundly impacts the pulmonary environment in rsv-infected mice characterized by the accumul ... | 2007 | 17442966 |
| adult asthma after non-respiratory syncytial virus bronchiolitis in infancy: subgroup analysis of the 20-year prospective follow-up study. | recent studies have stressed the influence of other viruses than respiratory syncytial virus (rsv) in the development of asthma in later childhood after bronchiolitis in infancy. however, the virus-specific prognosis until adulthood has remained obscure, due to lack of sufficiently long follow-up studies. the aim of the present study was to evaluate adult respiratory morbidity after bronchiolitis in infancy, focused on cases not caused by rsv. | 2007 | 17445037 |
| transcriptional profile of rous sarcoma virus transformed chicken embryo fibroblasts reveals new signaling targets of viral-src. | transformation of chicken fibroblasts in vitro by rous sarcoma virus represents a model of cancer in which a single oncogene, viral src, uniformly and rapidly transforms primary cells in culture. we experimentally surveyed the transcriptional program affected by rous sarcoma virus (rsv) in primary culture of chicken embryo fibroblasts. as a control, we used cells infected with non-transforming rsv mutant td106, in which the src gene was deleted. using affymetrix genechip chicken genome arrays, w ... | 2007 | 17448514 |
| common human toll-like receptor 4 polymorphisms--role in susceptibility to respiratory syncytial virus infection and functional immunological relevance. | evidence suggests that toll-like receptor 4 (tlr4) contributes to immune recognition of respiratory syncytial virus (rsv). the tlr4 gene harbours a polymorphism-asp299gly-previously associated with reduced tlr4 signalling. to understand of how host genetic variation influences the outcome of rsv infection in children, we examined the association between the tlr4 299gly allele and severe rsv disease. by genotyping 236 children with rsv infection and 219 healthy controls we found no association be ... | 2007 | 17449325 |
| [association of interleukin 8 single nucleotide polymorphisms with the susceptibility to respiratory syncytial virus infection]. | to explore the association of interleukin 8 (il-8)-251t/a and 781 c/t single nucleotide polymorphisms (snps) with the susceptibility of infants and young children to respiratory syncytial virus (rsv) infection. | 2007 | 17456336 |
| genetic variability of the fusion protein and circulation patterns of genotypes of the respiratory syncytial virus. | although antigenic and genetic variations were shown to occur both in the g and f protein of respiratory syncytial virus (rsv), few studies looked at the variation of f gene. the f genotypes were determined by the evaluation of clustering patterns, via the phylogenetic analysis of the nucleotide sequences of a variable region in the f gene. one hundred seventy-nine strains obtained from a children's hospital in korea over nine consecutive epidemics were included. the relationship between the f a ... | 2007 | 17457915 |
| respiratory syncytial virus infection in adults. | respiratory syncytial virus (rsv) is a common winter time respiratory virus that affects persons of all ages and is the major cause of serious lower respiratory tract infections in young children. however, rsv is also an important pathogen in adults, particularly in the elderly, patients with chronic lung disease, or those with impaired immunity. clinical features of rsv infections overlap with other respiratory viruses, so laboratory tests are required to establish the diagnosis. reverse transc ... | 2007 | 17458771 |
| [simultaneous outbreaks of rotavirus and respiratory syncytial virus in paris: a 12-year survey]. | the authors had for aim to study the coincidence of rsv and rotavirus epidemic peaks in pediatric patients hospitalized in the paris area. | 2007 | 17459636 |
| development of a 1-step enzyme-linked immunosorbent assay for the rapid diagnosis of bovine respiratory syncytial virus in postmortem specimens. | bovine respiratory syncytial virus (brsv) is associated with severe respiratory disease in cattle. brsv infection frequently leads to the death of young infected animals. the presence of brsv in postmortem specimens is routinely detected using indirect immunofluorescence (iif). however, this technique requires special equipment and considerable expertise. the present paper describes the development of a 1-step elisa for rapid (1.5 hours) detection of brsv antigen in organ homogenates. the perfor ... | 2007 | 17459851 |
| dna immunization with plasmids encoding fusion and nucleocapsid proteins of bovine respiratory syncytial virus induces a strong cell-mediated immunity and protects calves against challenge. | respiratory syncytial viruses (rsv) are one of the most important respiratory pathogens of humans and cattle, and there is currently no safe and effective vaccine prophylaxis. in this study, we designed two codon-optimized plasmids encoding the bovine rsv fusion (f) and nucleocapsid (n) proteins and assessed their immunogenicity in young calves. two administrations of both plasmids elicited low antibody levels but primed a strong cell-mediated immunity characterized by lymphoproliferative respon ... | 2007 | 17459933 |
| il-10 and rantes are elevated in nasopharyngeal secretions of children with respiratory syncytial virus infection. | respiratory syncytial virus (rsv) infection causes asthma-like symptoms in infants and young children. although an increase in several mediators in the airway during rsv infection has been reported, the mechanisms involved in airway inflammation are not fully understood. the aim of this study was to investigate the immunological deviation associated with airway inflammation by measuring cytokine and chemokine levels in the airway during rsv infection. | 2007 | 17460443 |
| effects of respiratory syncytial virus infection on dendritic cells and cysteinyl leukotrienes in lung tissues of a murine model of asthma. | pulmonary dendritic cells (dcs) play critical roles in both allergy and in viral infection. levels of cysteinyl leukotrienes (cyslts) increase after allergen sensitization and viral infection and can modulate the migration and functions of dcs. the present study examines the effects of respiratory syncytial virus (rsv) infection on numbers of dcs and cyslt concentrations in lung tissues of mice sensitized with mite allergen. | 2007 | 17460444 |
| promoter effects of adeno-associated viral vector for transgene expression in the cochlea in vivo. | the aims of this study were to evaluate the expression of enhanced green fluorescent protein (egfp) driven by 6 different promoters, including cytomegalovirus ie enhancer and chicken beta-actin promoter (cag), cytomegalovirus promoter (cmv), neuron-specific enolase promoter (nse), myosin 7a promoter (myo), elongation factor 1alpha promoter (ef-1alpha), and rous sarcoma virus promoter (rsv), and assess the dose response of cag promoter to transgene expression in the cochlea. serotype 1 adeno-asso ... | 2007 | 17464178 |
| cd14 -550 c/t, which is related to the serum level of soluble cd14, is associated with the development of respiratory syncytial virus bronchiolitis in the japanese population. | the contribution that genetic polymorphisms of toll-like receptor (tlr) 4 and of cd14--both of which recognize respiratory syncytial virus (rsv) in the innate immune response--make to rsv bronchiolitis in the japanese population has not yet been clarified. | 2007 | 17471431 |
| characterization of cd4+ memory t cell responses directed against common respiratory pathogens in peripheral blood and lung. | we investigated cd4(+) memory t cell responses to influenza virus (flu), respiratory syncytial virus (rsv), and nontypeable haemophilus influenzae (nthi). | 2007 | 17471443 |
| respiratory syncytial virus differentially activates murine myeloid and plasmacytoid dendritic cells. | respiratory syncytial virus (rsv) is the primary cause of bronchiolitis in young children. upon infection both t helper 1 (th1) and th2 cytokines are produced. because rsv-induced th2 responses have been associated with severe immunopathology and aggravation of allergic reactions, the regulation of the immune response following rsv infection is crucial. in this study we examined the influence of rsv on the activation and function of murine bone marrow-derived dendritic cells (dcs). rsv induced t ... | 2007 | 17472722 |
| bronchiolitis: can objective criteria predict eligibility for brief hospitalization? | to determine if 3 objective criteria - pulse oximetry, respiratory syncytial virus (rsv) testing, and age - could be used to predict which children hospitalized with bronchiolitis will have brief (<36 hour) hospitalizations and therefore be potential candidates for admission to short-stay observation units. | 2003 | 17472765 |
| does blood eosinophilia in wheezing infants predict later asthma? a prospective 18-20-year follow-up. | although eosinophilia in wheezing infants predicts subsequent wheezing, it is not known how long the association persists. our aim was to evaluate the connection between blood eosinophilia in infancy and later wheezing/asthma until adulthood, with special attention paid to infection-induced changes in blood eosinophils. we have prospectively followed up 83 infants until adulthood after hospitalization for bronchiolitis in 1981-1982. blood eosinophils were determined in a counting chamber on admi ... | 2007 | 17479599 |
| dynamics of filamentous viral rnps prior to egress. | the final step in the maturation of paramyxoviruses, orthomyxoviruses and viruses of several other families, entails the budding of the viral nucleocapsid through the plasma membrane of the host cell. many medically important viruses, such as influenza, parainfluenza, respiratory syncytial virus (rsv) and ebola, can form filamentous particles when budding. although filamentous virions have been previously studied, details of how viral filaments bud from the plasma membrane remain largely unknown ... | 2007 | 17485480 |
| human infant respiratory syncytial virus (rsv)-specific type 1 and 2 cytokine responses ex vivo during primary rsv infection. | respiratory syncytial virus (rsv) infection is the most common respiratory viral infection resulting in hospitalizations in infants worldwide. illness severity is likely multifactorial; however, unlike other viral infections, both type 1 and type 2 cytokine responses have been implicated in severe disease. | 2007 | 17492594 |
| neonatal human autologous dendritic cells pulsed with recombinant protein antigen prime the generation of non-polarized cd4 t-cell effectors. | the functional capability of human neonatal cd4 t cells to respond to vaccine antigens is frequently described as th2 biased, but whether this is due to defective t-cell or antigen-presenting cell (apc) function is unclear. in this study, we used purified t cells and autologous monocyte-derived dendritic cells (mddcs) as apcs to model primary and secondary neonatal cd4 t-cell responses in vitro to bbg2na, a recombinant protein subunit vaccine candidate against respiratory syncytial virus (rsv). ... | 2007 | 17493958 |
| function of the respiratory syncytial virus small hydrophobic protein. | respiratory syncytial virus (rsv), a member of the paramyxoviridae family, encodes a small hydrophobic (sh) protein of unknown function. parainfluenza virus 5 (piv5), a prototypical paramyxovirus, also encodes an sh protein, which inhibits tumor necrosis factor alpha (tnf-alpha) signaling. in this study, recombinant piv5 viruses without their own sh but containing rsv sh (from rsv strain a2 or b1) in its place (piv5deltash-rsv sh) and rsv lacking its own sh (rsvdeltash) were generated and analyz ... | 2007 | 17494063 |
| small interfering rna profiling reveals key role of clathrin-mediated endocytosis and early endosome formation for infection by respiratory syncytial virus. | respiratory syncytial virus (rsv) is a common cause of respiratory tract infections in infants and the elderly. like many other ph-independent enveloped viruses, rsv is thought to enter at the cell surface, independently of common endocytic pathways. we have used a targeted small interfering rna (sirna) library to identify key cellular genes involved in cytoskeletal dynamics and endosome trafficking that are important for rsv infection. surprisingly, rsv infection was potently inhibited by sirna ... | 2007 | 17494077 |
| eosinophils contribute to innate antiviral immunity and promote clearance of respiratory syncytial virus. | eosinophils are recruited to the lungs in response to respiratory syncytial virus (rsv) infection; however, their role in promoting antiviral host defense remains unclear. here, we demonstrate that eosinophils express tlrs that recognize viral nucleic acids, are activated and degranulate after single-stranded rna (ssrna) stimulation of the tlr-7-myd88 pathway, and provide host defense against rsv that is myd88 dependent. in contrast to wild-type mice, virus clearance from lung tissue was more ra ... | 2007 | 17495130 |
| antiviral activity of arbidol against influenza a virus, respiratory syncytial virus, rhinovirus, coxsackie virus and adenovirus in vitro and in vivo. | arbidol, ethyl-6-bromo-4-[(dimethylamino)-methyl]-5-hydroxy-1-methyl-2-[(phenylthio)methyl]-in dole-3-carboxylate hydrochloride monohydrate, is an antiviral chemical agent. in this report, we studied the antiviral activity of arbidol against a panel of human respiratory viruses, namely influenza a virus (flu-a, a/pr/8/34 h1n1), respiratory syncytial virus (rsv), human rhinovirus type 14 (hrv 14), coxsackie virus b3 (cvb3) and adenovirus type 7 (adv-7) in vitro in cell culture. arbidol was found ... | 2007 | 17497238 |
| surfactant protein b polymorphisms are associated with severe respiratory syncytial virus infection, but not with asthma. | surfactant proteins (sp) are important for the innate host defence and essential for a physiological lung function. several linkage and association studies have investigated the genes coding for different surfactant proteins in the context of pulmonary diseases such as chronic obstructive pulmonary disease or respiratory distress syndrome of preterm infants. in this study we tested whether sp-b was in association with two further pulmonary diseases in children, i. e. severe infections caused by ... | 2007 | 17498296 |
| vaccination with recombinant modified vaccinia virus ankara expressing bovine respiratory syncytial virus (brsv) proteins protects calves against rsv challenge. | respiratory syncytial virus (rsv) is a major cause of severe respiratory disease in infants and calves. bovine rsv (brsv) is a natural pathogen for cattle, and brsv infection in calves shares many features with the human infection. thus, brsv infection in cattle provides the ideal setting to evaluate the safety and efficacy of novel rsv vaccine strategies. here, we have evaluated the efficacy and safety of modified vaccinia virus ankara (rmva)-based vaccine candidates, expressing the bovine rsv- ... | 2007 | 17499893 |
| [sirna-mediated inhibition of respiratory syncytial virus replication]. | the effect of sirna against respiratory syncytial virus (rsv) was investigated in the cultured cells. ma104 cells were inoculated by rsv and transfected by different variants of sirna against rsv phosphoprotein (p) mrna or non-specific sirna as a control. the inhibition of rsv was assessed by microscopically studying the cells, titrating the virus, and estimating viral rna quantity in the culture supernatants by real-time polymerase chain reaction (pcr). the most potent variants of sirna caused ... | 2007 | 17500231 |
| respiratory virus infection among hematopoietic cell transplant recipients: evidence for asymptomatic parainfluenza virus infection. | the incidence of respiratory virus infection after hematopoietic cell transplantation (hct) has probably been underestimated with conventional testing methods in symptomatic patients. this prospective study assessed viral infection episodes by testing weekly respiratory samples collected from hct recipients, with and without symptoms reported by questionnaire, for 100 days after hct. samples were tested by culture and direct fluorescent antibody testing for respiratory syncytial virus (rsv), par ... | 2007 | 17502457 |
| interaction between respiratory syncytial virus and glycosaminoglycans, including heparan sulfate. | glycosaminoglycans (gags), including heparan sulfate (hs), are expressed on the surface of nearly all cells, linked to transmembrane proteins. these gags are sulfated to varying extents, lending a negative charge, and are used by a large number of viruses to initiate infection of immortalized cell lines. here we describe the rationale and methods for analyzing gag usage by one such virus, respiratory syncytial virus (rsv). the protocols presented allow the determination of which gag(s) is employ ... | 2007 | 17502668 |
| the use of two-dimensional sds-page to analyze the glycan heterogeneity of the respiratory syncytial virus fusion protein. | the respiratory syncytial virus (rsv) fusion (f) protein is synthesized as an inactive precursor (f0), which subsequently undergoes post-translational cleavage to give the disulphide bond-linked f1 and f2 subunits. the methodology detailing the use of two-dimensional electrophoresis, endoglycosidases, and alpha-mannosidase inhibitors, as applied to investigating f protein glycan maturation, is given. examples are used to show how this methodology was used to provide evidence for glycan heterogen ... | 2007 | 17502673 |
| the use of monoclonal antibodies and lectins to identify changes in viral glycoproteins that are influenced by glycosylation: the case of human respiratory syncytial virus attachment (g) glycoprotein. | the influence of viral envelope glycans is often overlooked, but one should bear in mind that variable glycosylation may affect the properties of viral envelope glycoproteins and potentially alter the course of an infection. hence, there is a need for simple methods that can be use to identify changes in the glycosylation pattern of viral glycoproteins in a large number of samples. we describe here methods for the analysis of cell-line specific changes in glycosylation of the respiratory syncyti ... | 2007 | 17502674 |
| secretion of the respiratory syncytial virus fusion protein from insect cells using the baculovirus expression system. | sequences derived from the respiratory syncytial virus (rsv) fusion (f) protein were expressed in insect cells as recombinant glutathione-s-transferase (gst)-tagged proteins. the sequence covering the f2 subunit (gst-f2), and a truncated form of the f protein in which the transmembrane domain was removed (gst-f2/f1), were cloned into the baculovirus pacsecg2t secretory vector. these virus sequences also had the endogenous virus signal sequence removed and replaced with a signal sequence derived ... | 2007 | 17502677 |
| what is the role of virus vaccination in patients with asthma? | it is estimated that viruses play a role in 30% to 80% of asthma exacerbations. thus, virus vaccination in patients with asthma could play an important role in preventing asthma exacerbations and other complications. influenza is the only agent for which a routine vaccine is currently available. this article discusses whether influenza vaccination in patients with asthma, based on the available evidence, is justified. cost-effectiveness of (influenza) vaccination for patients with asthma is ques ... | 2007 | 17504664 |
| simultaneous detection and high-throughput identification of a panel of rna viruses causing respiratory tract infections. | clinical presentations for viral respiratory tract infections are often nonspecific, and a rapid, high-throughput laboratory technique that can detect a panel of common viral pathogens is clinically desirable. we evaluated two multiplex reverse transcription-pcr (rt-pcr) products coupled with microarray-based systems for simultaneous detection of common respiratory tract viral pathogens. the ngen respiratory virus analyte-specific assay (nanogen, san diego, ca) detects influenza a virus (flu-a) ... | 2007 | 17507510 |
| [vronchiolitis and its treatment]. | bronchiolitis is the most common lower respiratory tract illness of infancy and is primarily caused by an infection with the respiratory syncytial virus (rsv). an inadequately elevated hospitalisation rate and the use of different treatments with unproven efficacy lead to high cost in terms of public health. implementation of evidence based guidelines can reduce these costs but not parents satisfaction and quality of care. palivizumab is a preventive medication with specific indications. post-br ... | 2007 | 17508665 |
| atypical measles and enhanced respiratory syncytial virus disease (erd) made simple. | atypical measles and enhanced respiratory syncytial virus disease (erd) were serious diseases that resulted from exposure of children immunized with inactivated vaccines against measles virus (mv) and respiratory syncytial virus (rsv) to the respective wild-type agents in the 1960s. although the clinical manifestations of both illnesses were different, the immune responses elicited and primed for by the vaccines shared important similarities. both vaccines failed to elicit long-lived protective ... | 2007 | 17515829 |
| rsv mediates pseudomonas aeruginosa binding to cystic fibrosis and normal epithelial cells. | cystic fibrosis lung disease typically has a course of exacerbations and remissions, suggesting that external factors like viral infections can influence this course. clinical data suggest synergism between respiratory syncytial virus (rsv) infections and pseudomonas aeruginosa in cystic fibrosis (cf) lung disease. we studied the influence of rsv infection on adherence of p. aeruginosa to ib3-1, hep-2, and a549 epithelial cell monolayers in vitro. rsv infection of epithelial cells as well as sim ... | 2007 | 17515861 |
| coupled translation of the second open reading frame of m2 mrna is sequence dependent and differs significantly within the subfamily pneumovirinae. | coupled translation, first described in the m2 gene of pneumovirus respiratory syncytial virus (rsv), is an alternative mechanism of translational initiation in which the ribosomes which translate the first (m2-1) open reading frame (orf) move a short distance upstream after termination and reinitiate translation from a second (m2-2) overlapping orf. here, we show that the same mechanism occurs in two closely related viruses, avian pneumovirus (apv) and pneumonia virus of mice (pvm), although wi ... | 2007 | 17522208 |
| hospitalized children with respiratory syncytial virus infection and neuromuscular impairment face an increased risk of a complicated course. | respiratory syncytial virus (rsv) infection is an important cause of viral respiratory tract infection in children. in contrast to other confirmed risk factors that predispose to a higher morbidity and mortality, the particular risk of a preexisting neuromuscular impairment (nmi) in hospitalized children with rsv infection has not been prospectively studied in a multicenter trial. | 2007 | 17529864 |
| antibiotic use in children is not influenced by the result of rapid antigen detection test for the respiratory syncytial virus. | rapid antigen detection test (radt) for respiratory syncytial virus (rsv) is widely used in children hospitalized with acute respiratory tract infection (arti), but its influence on antibiotic (ab) use is uncertain. | 2007 | 17532258 |
| high-throughput, sensitive, and accurate multiplex pcr-microsphere flow cytometry system for large-scale comprehensive detection of respiratory viruses. | human respiratory viruses are a diverse group of pathogens composed of hundreds of virus strains, and this presents a major challenge for diagnostic laboratories. to efficiently detect numerous viruses in a large epidemiologic study, we developed a fast, multitarget, sensitive, and specific assay named the respiratory multicode-plx assay (rma). the rma utilizes improved multiplex pcr chemistry (eragen multicode-plx technology) coupled with high-throughput microsphere flow cytometry (luminex). ei ... | 2007 | 17537928 |
| post-infection a77-1726 blocks pathophysiologic sequelae of respiratory syncytial virus infection. | despite respiratory syncytial virus (rsv) bronchiolitis remaining the most common cause of lower respiratory tract disease in infants worldwide, treatment has progressed little in the past 30 years. the aim of our study was to determine whether post-infection administration of de novo pyrimidine synthesis inhibitors could prevent the reduction in alveolar fluid clearance (afc) and hypoxemia that occurs at day 2 after intranasal infection of balb/c mice with rsv. balb/c mice were infected intrana ... | 2007 | 17541010 |
| respiratory syncytial virus infection in infants admitted to paediatric intensive care units in london, and in their families. | we carried out a study in five london paediatric intensive care units (picus), with the objectives of describing a cohort of infants with respiratory syncytial virus (rsv) infection, comparing hospital diagnosis with pcr results and investigating the spread of rsv in families. eligible infants were under 5 months old and admitted betweem november 1998 and october 1999 with respiratory failure, apnoea and/or bradycardia or acute life threatening episodes (alte). we diagnosed rsv by pcr analysis o ... | 2008 | 17541638 |
| chemokines and their receptors in respiratory disease: a therapeutic target for respiratory syncytial virus infection. | cell recruitment is a multistep process orchestrated by chemokines and their receptors. the chemokine/receptor system is central to many inflammatory diseases, making it a key target for therapeutic intervention. despite complexity and redundancy within the system, effective antagonists are in development and undergoing clinical trials, for example, maraviroc, for use in hiv treatment. respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infection in infants, with global ... | 2007 | 17547506 |
| expression of rna virus proteins by rna polymerase ii dependent expression plasmids is hindered at multiple steps. | proteins of human and animal viruses are frequently expressed from rna polymerase ii dependent expression cassettes to study protein function and to develop gene-based vaccines. initial attempts to express the g protein of vesicular stomatitis virus (vsv) and the f protein of respiratory syncytial virus (rsv) by eukaryotic promoters revealed restrictions at several steps of gene expression. | 2007 | 17550613 |
| a prospective three-year cohort study of the epidemiology and virology of acute respiratory infections of children in rural india. | acute respiratory infection (ari) is a major killer of children in developing countries. although the frequency of ari is similar in both developed and developing countries, mortality due to ari is 10-50 times higher in developing countries. viruses are common causes of ari among such children, yet the disease burden of these infections in rural communities is unknown. | 2007 | 17551572 |
| mapk and heat shock protein 27 activation are associated with respiratory syncytial virus induction of human bronchial epithelial monolayer disruption. | respiratory syncytial virus (rsv) is the major cause of bronchiolitis in infants, and a common feature of rsv infections is increased lung permeability. the accumulation of fluid in the infected lungs is caused by changes in the endothelial and epithelial membrane integrity. however, the exact mechanisms of viral-induced fluid extravasation remain unclear. here, we report that infection of human epithelial cells with rsv results in significant epithelial membrane barrier disruption as assessed b ... | 2007 | 17557802 |
| [diagnosis and epidemiological surveillance of influenza and respiratory syncytial virus infections: interest of multiplex pcr]. | respiratory infections require a rapid etiological diagnosis for efficient management of cases. we evaluated multiplex pcr used for the diagnosis and the epidemiological surveillance of influenza and respiratory syncytial virus (rsv) infections. | 2007 | 17560747 |
| intranasal proteosome-based respiratory syncytial virus (rsv) vaccines protect balb/c mice against challenge without eosinophilia or enhanced pathology. | a safe and effective vaccine against respiratory syncytial virus (rsv) is still unavailable. proteosome-based adjuvants are derived from the outer membrane proteins (omp) of neisseria species and are potent inducers of both mucosal and systemic immunity in humans and animals. candidate rsv subunit vaccines comprising enriched rsv proteins (ersv) formulated with proteosomes alone or with lps (protollin) were produced. administered intranasally in balb/c mice, both vaccines elicited long-lasting s ... | 2007 | 17561317 |
| the burden of respiratory viral disease in hospitalized children in paris. | a virus was identified in 464 out of 1,212 patients, 8 days to 16 years of age, who were admitted to the hospital in paris during a 3 year period. respiratory syncytial virus (rsv) was found in 428 patients, 74% were younger than 6 months with bronchiolitis, and 6% were older than 2 years. rsv was the first virus isolated in all patients and in those with pneumonia or asthma. the low number of admissions due to parainfluenzae viruses is characteristic of this area compared to other countries. | 2008 | 17562076 |
| the 24-angstrom structure of respiratory syncytial virus nucleocapsid protein-rna decameric rings. | respiratory syncytial virus (rsv), a nonsegmented, negative-sense rna-containing virus, is a common cause of lower respiratory tract disease. expression of rsv nucleocapsid protein (n) in insect cells using the baculovirus expression system leads to the formation of n-rna complexes that are morphologically indistinguishable from viral nucleocapsids. when imaged in an electron microscope, three distinct types of structures were observed: tightly wound short-pitch helices, highly extended helices, ... | 2007 | 17567697 |
| [acute bronchiolitis, an updated review]. | acute bronchiolitis (ab) is a frequent cause of hospitalization among children and its main etiological agent is respiratory syncytial virus (rsv). it occurs epidemically during autumn and winter. some populations of children such as premature newborns, infants with congenital heart disease and those with chronic lung disease, immunocompromised, undernourished, among others, present increased morbidity and mortality risk. the virus multiplies in epithelial ciliated cells while inflammation and c ... | 2007 | 17568926 |
| evaluation of compliance with palivizumab recommendations in a multicenter study of young children presenting to the emergency department with bronchiolitis. | monthly palivizumab injections from november to march decrease risk of respiratory syncytial virus (rsv)-related hospitalization during rsv season in high-risk infants born less than 35 weeks of gestation. our objective was to investigate compliance with the american academy of pediatrics (aap) recommendations for palivizumab prophylaxis among children who present to the emergency department (ed) with bronchiolitis. | 2007 | 17572518 |
| respiratory syncytial virus fusion inhibitors. part 5: optimization of benzimidazole substitution patterns towards derivatives with improved activity. | extensive sar studies and optimization of adme properties of benzimidazol-2-one derivatives led to the identification of bms-433771 (3) as an orally active rsv fusion inhibitor. in order to extend the structure-activity relationships for this compound series, substitution of the benzimidazole ring was examined with a view to establishing additional productive interactions between the inhibitor and functionality present in the proposed binding pocket. amongst the compounds synthesized, the 5-amin ... | 2007 | 17576060 |
| rsv604, a novel inhibitor of respiratory syncytial virus replication. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract infections worldwide, yet no effective vaccine or antiviral treatment is available. here we report the discovery and initial development of rsv604, a novel benzodiazepine with submicromolar anti-rsv activity. it proved to be equipotent against all clinical isolates tested of both the a and b subtypes of the virus. the compound has a low rate of in vitro resistance development. sequencing revealed that the resis ... | 2007 | 17576833 |
| tlr4 polymorphisms mediate impaired responses to respiratory syncytial virus and lipopolysaccharide. | severe bronchiolitis following respiratory syncytial virus (rsv) infection occurs in only a small subset of infected infants and the basis for variations in disease severity is not understood. innate immune responses to rsv are mediated by tlr-4, and the (299)gly and (399)ile alleles of the tlr4 gene have been linked epidemiologically with increased severity of rsv disease in children. we hypothesized that cellular immune responses to rsv mediated by these variant forms of the receptor are defec ... | 2007 | 17579031 |
| can myoglobin expression in pancreatic beta cells improve insulin secretion under hypoxia? an exploratory study with transgenic porcine islets. | the feasibility of myoglobin (mb)-facilitated oxygen transport in improving porcine islet survival under hypoxia was investigated. discrete groups of islets were transfected with replication-defective adenoviral vector ad5 respiratory syncitial virus (rsv) to induce expression of mb or green fluorescent protein (gfp). native islets served as the controls. in vitro studies at 37 degrees c assessed islet insulin secretion efficacy: (i) to a glucose challenge from 30 to 300 mg/dl at fixed po2; and ... | 2007 | 17584476 |
| genomic analysis of rice stripe virus zhejiang isolate shows the presence of an otu-like domain in the rna1 protein and a novel sequence motif conserved within the intergenic regions of ambisense segments of tenuiviruses. | the complete genome sequence of the four rnas of rice stripe virus zhejiang isolate was determined. in addition to polymerase modules, the pc1 protein encoded on rna1 harbours an ovarian tumour (otu) - like cysteine protease signature near its n-terminus, suggesting that the protein might yield the viral polymerase and one or more additional proteins by autoproteolytic cleavage and/or have deubiquitination activity. a novel inverted repeat sequence motif was found to be universal within the inte ... | 2007 | 17585367 |
| winter air pollution and infant bronchiolitis in paris. | respiratory syncytial virus (rsv) is one of the most common respiratory pathogens in infants and young children. it is not known why some previously healthy infants, when in contact with rsv, develop bronchiolitis whereas others have only mild symptoms. our study aimed to evaluate the possible association between emergency hospital visits for bronchiolitis and air pollution in the paris region during four winter seasons. we included children under the age of 3 years who attended emergency room s ... | 2008 | 17585899 |
| palivizumab prophylaxis, respiratory syncytial virus, and subsequent recurrent wheezing. | children who experience respiratory syncytial virus (rsv) lower respiratory tract infections (lrtis) early in life have high rates of subsequent recurrent wheezing. palivizumab, an anti-rsv monoclonal antibody, has 78% to 80% efficacy in preventing rsv hospitalization in premature infants without chronic lung disease. we hypothesized that palivizumab, by ameliorating or preventing early rsv lrti in preterm infants, might decrease later recurrent wheezing. | 2007 | 17586188 |
| impact of human metapneumovirus and respiratory syncytial virus co-infection in severe bronchiolitis. | we have previously shown high rates of co-infection with respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) in infants with severe bronchiolitis at our institution in 2000-2002, and that co-infection was associated with increased disease severity. in this study, we have attempted to identify differences in intubated infants with severe rsv infection with and without hmpv co-infection. here we show that rsv+/hmpv+ were clinically symptomatic for longer than rsv+/hmpv- infants, but ... | 2007 | 17588245 |
| respiratory syncytial virus infects and abortively replicates in the lungs in spite of preexisting immunity. | respiratory syncytial virus (rsv) is a major cause of bronchiolitis and viral pneumonia in young children and a serious health risk in immunocompromised individuals and the elderly. immunity to rsv is not completely understood. in this work, we established a method for monitoring rsv infection by real-time pcr and applied this method for analysis of rsv replication in vivo in the cotton rat model in naïve animals and in animals rendered immune to rsv by prior rsv infection. we found that even th ... | 2007 | 17596309 |
| cord blood gene expression in infants hospitalized with respiratory syncytial virus bronchiolitis. | only a few infants develop acute bronchiolitis when exposed to respiratory syncytial virus (rsv), and host, environmental, and viral properties are probably all of importance in determining the severity of infection. | 2007 | 17597454 |
| respiratory syncytial virus recombinant f protein (residues 255-278) induces a helper t cell type 1 immune response in mice. | we have developed and evaluated an immunodominant respiratory syncytial virus (rsv) f antigen in a mouse model. the antigenic region corresponding to amino acids 255-278 of the rsv f protein was cloned into a vector containing the ctxa(2)b gene of cholera toxin (ct). the recombinant protein was expressed in escherichia coli and analyzed on sodium dodecyl sulfate-polyacrylamide gels. the purified protein was evaluated by immunoblot and ganglioside gm(1) enzyme-linked immunosorbent assay to confir ... | 2007 | 17603843 |
| kinetics of antibody-induced modulation of respiratory syncytial virus antigens in a human epithelial cell line. | the binding of viral-specific antibodies to cell-surface antigens usually results in down modulation of the antigen through redistribution of antigens into patches that subsequently may be internalized by endocytosis or may form caps that can be expelled to the extracellular space. here, by use of confocal-laser-scanning microscopy we investigated the kinetics of the modulation of respiratory syncytial virus (rsv) antigen by rsv-specific igg. rsv-infected human epithelial cells (hep-2) were incu ... | 2007 | 17608950 |
| influenza and respiratory syncytial virus in infants and children: relationship with attendance at a paediatric emergency unit and characteristics of the circulating strains. | a study was carried out on 2,696 italian children, aged 0-14 years. the goals were: (1) to define the age-related impact of acute respiratory infections (ari), measured as the risk of attendance at the paediatric emergency room, (2) to better define the importance and proportion of influenza and respiratory syncytial virus (rsv) infections and (3) to acquire deeper knowledge of the influenza strains circulating in infants and children. a standardised emergency unit attendance risk (euar) was cal ... | 2007 | 17610094 |
| rapid and highly sensitive qualitative real-time assay for detection of respiratory syncytial virus a and b using nasba and molecular beacon technology. | the performance of a sensitive and specific qualitative respiratory syncytial virus (rsv) assay based on nasba technology and real-time molecular beacon detection is presented. very low detection limits for both rsv a and rsv b were determined: 95% detection hit-rate of 95 and 47 copies/input in isolation for rsv a and rsv b, respectively. rsv was detected in a wide variety of clinical samples including respiratory swabs, nasopharyngeal aspirates (npa), bronchoalveolar lavages (bal), endotrachea ... | 2007 | 17614146 |
| transmission of rice stripe virus acquired from frozen infected leaves by the small brown planthopper (laodelphax striatellus fallen). | rice stripe disease, caused by rice stripe virus (rsv), is one of the most serious rice diseases in temperate and subtropical regions of the world. since rsv is not transmissible mechanically, an insect transmission test was the original basis for identification of the viral population and cultivar resistance. a simple, rapid and reliable method is described by which virus-free small brown planthoppers acquired rsv from frozen infected rice leaves and transmitted the virus to healthy rice plants ... | 2007 | 17614147 |
| respiratory syncytial virus fusion inhibitors. part 6: an examination of the effect of structural variation of the benzimidazol-2-one heterocycle moiety. | the effect of structural variation of the benzimidazol-2-one ring of rsv fusion inhibitors related to bms-433771 (1) was examined in conjunction with side chain modifications and the introduction of an aminomethyl substituent at the 5-position of the core benzimidazole moiety. replacement of the benzimidazol-2-one moiety with benzoxazole, oxindole, quinoline-2-one, quinazolin-2,4-dione and benzothiazine derivatives provided a series of potent rsv fusion inhibitors 4. however, the intrinsic poten ... | 2007 | 17616396 |
| intratumoral expression of respiratory syncytial virus fusion protein in combination with cytokines encoded by adenoviral vectors as in situ tumor vaccine for colorectal cancer. | although cancers can naturally elicit immune responses, immune ignorance is a common observation preventing immune-mediated elimination of tumor cells. we assessed whether intratumoral expression of respiratory syncytial virus fusion (rsv-f) protein, encoded by a replication-defective adenovirus vector (ad.rsv-f), alone or in combination with local coexpression of cytokines can induce tumor-specific immune responses in a syngeneic murine colon cancer model. we confirmed in vitro by dye colocaliz ... | 2007 | 17620425 |
| respiratory syncytial virus rhinosinusitis in intensive care unit patients. | this study reported a case of rhinosinusitis for respiratory syncytial virus in intensive care unit patient. the settings were intensive care unit at hospital das clínicas, university of são paulo medical school, são paulo, brazil. one female hiv-infected patient with respiratory failure and circulatory shock due to splenic and renal abscesses, who developed rhinosinusitis caused by rsv and bacteria. respiratory viruses can play a pathogenic role in airways infection allowing secondary bacterial ... | 2007 | 17625748 |
| differential type i interferon induction by respiratory syncytial virus and influenza a virus in vivo. | type i interferon (ifn) induction is an immediate response to virus infection, and very high levels of these cytokines are produced when the toll-like receptors (tlrs) expressed at high levels by plasmacytoid dendritic cells (pdcs) are triggered by viral nucleic acids. unlike many rna viruses, respiratory syncytial virus (rsv) does not appear to activate pdcs through their tlrs and it is not clear how this difference affects ifn-alpha/beta induction in vivo. in this study, we investigated type i ... | 2007 | 17626092 |
| identification of a cd4 t cell epitope in the pneumonia virus of mice glycoprotein and characterization of its role in protective immunity. | pneumonia virus of mice (pvm) causes bronchiolitis and pneumonia in mice. infection is associated with high levels of viral replication in the lungs and results in the functional inactivation of pulmonary virus-specific cd8 t cells. due to its similarity to severe human respiratory syncytial virus (rsv) infection, pvm infection in mice has been proposed as an alternative rsv model. here, we have delineated the minimal requirements for protective t cell immunity in the pvm model. immunization wit ... | 2007 | 17632195 |
| replacement of the respiratory syncytial virus nonstructural proteins ns1 and ns2 by the v protein of parainfluenza virus 5. | paramyxoviruses have been shown to produce proteins that inhibit interferon production and signaling. for human respiratory syncytial virus (rsv), the nonstructural ns1 and ns2 proteins have been shown to have interferon antagonist activity through an unknown mechanism. to understand further the functions of ns1 and ns2, we generated recombinant rsv in which both ns1 and ns2 were replaced by the piv5 v protein, which has well-characterized ifn antagonist activities (deltans1/2-v). expression of ... | 2007 | 17632199 |
| overlapping roles of the rous sarcoma virus gag p10 domain in nuclear export and virion core morphology. | nucleocytoplasmic shuttling of the rous sarcoma virus (rsv) gag polyprotein is an integral step in virus particle assembly. a nuclear export signal (nes) was previously identified within the p10 domain of rsv gag. gag mutants containing deletions of the p10 nes or mutations of critical hydrophobic residues at positions 219, 222, 225, or 229 become trapped within the nucleus and exhibit defects in the efficiency of virus particle release. to investigate other potential roles for gag nuclear traff ... | 2007 | 17634229 |
| withdrawn: immunoglobulin for preventing respiratory syncytial virus infection. | respiratory syncytial virus, the most important cause of lower respiratory tract infections in infants and young children in industrialized countries, is associated with increased morbidity in premature infants with or without bronchopulmonary dysplasia as well as those with congenital heart disease. because of observations that lower rates of disease occur immediately after birth, presumably due to vertical transmission of maternal antibody, and animal studies where protection from pneumonia wa ... | 2007 | 17636681 |
| respiratory syncytial virus: seasonal data for regions of florida and implications for palivizumab. | the aims of this study are to define the length of the respiratory syncytial virus (rsv) season throughout florida and evaluate the american academy of pediatrics (aap) guidelines on the administration of palivizumab in light of the florida data. | 2007 | 17639745 |
| respiratory syncytial virus synergizes with th2 cytokines to induce optimal levels of tarc/ccl17. | respiratory syncytial virus (rsv) is a ubiquitous virus that preferentially infects airway epithelial cells, causing asthma exacerbations and severe disease in immunocompromised hosts. acute rsv infection induces inflammation in the lung. thymus- and activation-regulated chemokine (tarc) recruits th2 cells to sites of inflammation. we found that acute rsv infection of balb/c mice increased tarc production in the lung. immunization of balb/c mice with individual rsv proteins can lead to the devel ... | 2007 | 17641031 |
| co-infection of human metapneumovirus with adenovirus or respiratory syncytial virus among children in japan. | human metapneumovirus (hmpv) is one of the etiological agents of acute respiratory tract infections. from june 2005 to may 2006, we collected 185 clinical specimens from children in osaka city, japan, and detected 41 hmpv rna. of the 41 specimens, four (9.8%) also contained other viruses (3 with adenovirus [adv] and 1 with respiratory syncytial virus [rsv]). the clinical symptoms of patients coinfected with adv were indistinct from those of patients mono-infected with hmpv. the symptoms of the o ... | 2007 | 17641470 |
| [effect of leukotriene receptor antagonist on the levels of th1 and th2 cytokines in sera of infants with respiratory syncytial virus pneumonia]. | to investigate the effects of leukotriene receptor antagonist on the levels of th1 and th2 cytokines and the serum cysteinyl leukotrenes (cyslts) in infants and young children with respiratory syncytial virus (rsv) pneumonia. | 2007 | 17653314 |
| monoclonal antibodies in pediatrics: use in prevention and treatment. | an update is provided on monoclonal antibodies (mabs): concept, production, indications for the diagnosis and treatment of neoplastic diseases and autoimmune disorders, prevention of transplant rejection, and treatment of allergic diseases, autoimmune disease and other noninflammatory disorders such as coronary disease. mention is also made of mab use in the prevention of respiratory syncytial virus (rsv) infection. a more extensive account is provided of the use of mab in b cell lymphomas (anti ... | 2007 | 17663923 |