Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| the impact of abiotic factors (temperature and glucose) on physicochemical properties of lipids from yersinia pseudotuberculosis. | the impact of the availability of glucose in nutrition medium and growth temperature on the composition and thermotropic behavior of lipids from yersinia pseudotuberculosis (enterobacteriaceae) was studied. y. pseudotuberculosis was grown in nutrition broth (nb) with/without glucose at 8 and 37 degrees c, corresponding to the temperatures of saprophytic and parasitic phases of this bacterium life. the decrease of phosphatidylethanolamine, phosphatidylglycerol and unsaturated fatty acids and the ... | 2004 | 15667937 |
| the flag-2 locus, an ancestral gene cluster, is potentially associated with a novel flagellar system from escherichia coli. | escherichia coli k-12 possesses two adjacent, divergent, promoterless flagellar genes, fhia-mbha, that are absent from salmonella enterica. through bioinformatics analysis, we found that these genes are remnants of an ancestral 44-gene cluster and are capable of encoding a novel flagellar system, flag-2. in enteroaggregative e. coli strain 042, there is a frameshift in lfgc that is likely to have inactivated the system in this strain. tiling path pcr studies showed that the flag-2 cluster is pre ... | 2005 | 15687208 |
| brothers in arms. | 2005 | 15693175 | |
| bacterial toxins and the immune system: show me the in vivo targets. | microorganisms that cause persistent infection often exhibit specific adaptations that allow them to avoid the adaptive immune response. recently, several bacterial toxins have been shown in vitro to disrupt immune cell functions. however, it remains to be established whether these activities are relevant during infection and whether these toxins have specifically evolved to disrupt the adaptive immune system. | 2005 | 15699067 |
| the adaptor molecules lat and slp-76 are specifically targeted by yersinia to inhibit t cell activation. | t cell responses are critical to the survival of yersinia-infected animals. yersinia have the ability to directly suppress t lymphocyte activation through the virulence factor yoph, a tyrosine phosphatase. using single cell video microscopy and facs analysis, here we show that even an average of one yersinia per t cell is sufficient to inhibit or alter t cell responses. this efficient inhibition is traced to specific targeting by yoph of the adaptor proteins, linker for activation of t cells (la ... | 2005 | 15699071 |
| [three cases of yersinia pseudotuberculosis gastrointestinal infection having no apparent epidemiological relationship, caused by identical strains]. | gastrointestinal infections caused by yersinia pseudotuberculosis are uncommon in our country and the epidemiology of the infection is uncertain. | 2005 | 15701328 |
| [bacteriemia and hepatic abscess secondary to yersinia pseudotuberculosis infection]. | 2005 | 15701336 | |
| pseudogene accumulation might promote the adaptive microevolution of yersinia pestis. | plague is a natural focus-based disease, and for better understanding of this disease it is crucial to determine the molecular mechanisms of its pathogen, yersinia pestis, for adapting to different foci. gene inactivation, loss and acquisition are the main mechanisms that contribute to a pathogen's fitness. determination of the whole-genome sequences of three y. pestis strains, co92, kim and 91001, provided a good opportunity to probe into its genome in minute detail. many genetic variations wer ... | 2005 | 15713609 |
| two substrate-targeting sites in the yersinia protein tyrosine phosphatase co-operate to promote bacterial virulence. | yoph is a protein tyrosine phosphatase and an essential virulence determinant of the pathogenic bacterium yersinia. yersinia delivers yoph into infected host cells using a type iii secretion mechanism. yoph dephosphorylates several focal adhesion proteins including p130cas in human epithelial cells, resulting in disruption of focal adhesions and cell detachment from the extracellular matrix. how the c-terminal protein tyrosine phosphatase domain of yoph targets specific substrates such as p130ca ... | 2005 | 15720545 |
| turning yersinia pathogenesis outside in: subversion of macrophage function by intracellular yersiniae. | three bacterial species within the genus yersinia are causative agents of human disease. yersinia pestis is transmitted by fleas or in aerosols, infects regional lymph nodes or lungs, and causes the highly lethal disease known as plague. yersinia enterocolitica and yersinia pseudotuberculosis are enteric pathogens most commonly associated with self-limiting infections of the mesenteric lymph nodes. although y. pestis and the enteropathogenic yersinia species utilize different modes of transmissi ... | 2005 | 15721832 |
| crispr elements in yersinia pestis acquire new repeats by preferential uptake of bacteriophage dna, and provide additional tools for evolutionary studies. | the remarkable repetitive elements called crisprs (clustered regularly interspaced short palindromic repeats) consist of repeats interspaced with non-repetitive elements or 'spacers'. crisprs are present in both archaea and bacteria, in association with genes involved in dna recombination and repair. in the yersinia pestis genome, three such elements are found at three distinct loci, one of them being highly polymorphic. the authors have sequenced a total of 109 alleles of the three y. pestis cr ... | 2005 | 15758212 |
| quantitative microarray-based dna-dna hybridization assay for measuring genetic distances among bacterial species and its application to the identification of family enterobacteriaceae. | quantitative dna-dna hybridization to measure the genetic distances among bacterial species is indispensable for taxonomical determination. in the current studies, we developed a method to determine bacterial dna relatedness on a glass microarray. reference dnas representing a total 93 species of enterobacteriaceae were arrayed on a glass microplate, and signal intensities were measured after 2 hr of hybridization with cy3-labeled bacterial dnas. all immobilized dnas from members of the family e ... | 2005 | 15781999 |
| yersinia pestis v protein epitopes recognized by cd4 t cells. | pneumonic plague, an often-fatal disease for which no vaccine is presently available, results from pulmonary infection by the bacterium yersinia pestis. the y. pestis v protein is a promising vaccine candidate, as v protein immunizations confer to mice significant protection against aerosolized y. pestis. cd4 t cells play central roles during vaccine-primed immune responses, but their functional contributions to y. pestis vaccines have yet to be evaluated and optimized. toward that end, we repor ... | 2005 | 15784563 |
| role of predicted transmembrane domains for type iii translocation, pore formation, and signaling by the yersinia pseudotuberculosis yopb protein. | yopb is a 401-amino-acid protein that is secreted by a plasmid-encoded type iii secretion system in pathogenic yersinia species. yopb is required for yersinia spp. to translocate across the host plasma membrane a set of secreted effector proteins that function to counteract immune signaling responses and to induce apoptosis. yopb contains two predicted transmembrane helices (residues 166 to 188 and 228 to 250) that are thought to insert into the host plasma membrane during translocation. yopb is ... | 2005 | 15784589 |
| linkage of the horizontally acquired ypm and pil genes in yersinia pseudotuberculosis. | the superantigen-encoding ypm gene and the pil gene cluster governing type iv pilus biogenesis have been laterally acquired by yersinia pseudotuberculosis. pcr assays on 270 unrelated strains from various environmental and animal sources revealed a significant association of ypm and pil in isolates. | 2005 | 15784605 |
| role of macrophage apoptosis in the pathogenesis of yersinia. | yersinia species that are pathogenic for humans (yersinia pestis, yersinia pseudotuberculosis, and yersinia enterocolitica) induce apoptosis in macrophages. yersinia-induced apoptosis utilizes the mitochondrial pathway and is executed by activation of caspase cascades. the mechanism of yersinia-induced apoptosis in macrophages has two essential components. one component is the innate immune response of macrophages to the pathogen, which leads to the activation of a survival response and a death ... | 2005 | 15791955 |
| engineered e. coli delivers therapeutic genes to the colonic mucosa. | taking advantage of the proximity of bowel mucosa to luminal bacteria, we have attempted to deliver a therapeutic gene to the colonic mucosa by oral administration of an invasive and non-pathogenic escherichia coli. e. coli diamenopimelate (dap) auxotroph, harboring plasmid pgb2omegainv-hly, express the inv gene from yersinia pseudotubercolosis that confers the ability to invade nonprofessional phagocytic cells and the hly gene from listeria monocytogenes that allows expression of lystreriolysin ... | 2005 | 15815705 |
| subcellular proteomic analysis of host-pathogen interactions using human monocytes exposed to yersinia pestis and yersinia pseudotuberculosis. | yersinia pestis, the etiological agent of plague, is of concern to human health both from an infectious disease and a biodefense perspective. while y. pestis and yersinia pseudotuberculosis share more than 90% dna homology, they have significantly different clinical manifestations. plague is often fatal if untreated, yet y. pseudotuberculosis causes severe intestinal distress but is rarely fatal. a better understanding of host response to these closely related pathogens may help explain the diff ... | 2005 | 15825148 |
| observation of the intracellular behavior of recombinant yersinia pseudotuberculosis invasin protein. | in this study, we observed the intracellular behavior of recombinant invasin, a 103-kda outer membrane protein of yersinia pseudotuberculosis. to mimic the in vivo behavior of bacterial invasin, a polyvalent form of invasin was generated by incubation of biotinylated gst-fused invasin c-terminal portion protein (gst-invs) with avidin. several experiments confirmed that the recombinant invasin could consistently reproduce the invasin-mediated entry to mammalian epithelial cells. we analyzed the m ... | 2005 | 15840954 |
| small-molecule inhibitors specifically targeting type iii secretion. | the type iii secretion (tts) system is used by several animal and plant pathogens to deliver effector proteins into the cytosol of the eukaryotic target cell as a strategy to evade the defense reactions elicited by the infected organism. the fact that these systems are highly homologous implies that novel antibacterial agents that chemically attenuate the pathogens via a specific interaction with the type iii secretion mechanism can be identified. a number of small organic molecules having this ... | 2005 | 15845518 |
| a novel semiquantitative fluorescence-based multiplex polymerase chain reaction assay for rapid simultaneous detection of bacterial and parasitic pathogens from blood. | a multiplex polymerase chain reaction assay was developed for the rapid simultaneous detection of category a select bacterial agents (bacillus anthracis and yersinia pestis) and parasitic pathogens (leishmania species) in blood using the cepheid smart cycler platform. b. anthracis (sterne) and yersinia. pseudotuberculosis were used in the assay for optimization for b. anthracis and y. pestis, respectively. the specificity of the target amplicons [protective antigen gene of b. anthracis and rrna ... | 2005 | 15858151 |
| genetic variations in the pgm locus among natural isolates of yersinia pestis. | a pcr-based screening method was used to study the genetic variations of the pgm locus among natural isolates of yersinia pestis from china. our results indicate that genetic variations in the pgm locus are well correlated with biovars of y. pestis and plague foci, suggesting that the pgm locus plays a role in y. pestis adaptation to its environment. the gene encoding two-component regulatory system sensor kinase became a pseudogene in all strains of biovar orientalis due to a thymidine deletion ... | 2005 | 15864756 |
| horizontal transfer of the high-pathogenicity island of yersinia pseudotuberculosis. | the horizontal transfer of genetic elements plays a major role in bacterial evolution. the high-pathogenicity island (hpi), which codes for an iron uptake system, is present and highly conserved in various enterobacteriaceae, suggesting its recent acquisition by lateral gene transfer. the aim of this work was to determine whether the hpi has kept its ability to be transmitted horizontally. we demonstrate here that the hpi is indeed transferable from a donor to a recipient yersinia pseudotubercul ... | 2005 | 15866919 |
| [influence of gaseous metabolites of soil bacteria on the multiplication of listeria monocytogenes and yersinia pseudotuberculosis]. | the influence of gaseous metabolites of saprophytic soil bacteria on the growth and multiplication of l. monocytogenes and y. pseudotuberculosis was studied. the study revealed that all cultures under study exhibited selectively both inhibiting and stimulating action on the multiplication of test cultures. bacteria of the genera pseudomonas and acinetobacter showed the highest inhibiting activitywith respect to the test cultures. volatile metabolites of bacteria of the genus aeromonas showed the ... | 2005 | 15881930 |
| [yersinia pseudotuberculosis nucleoside-kinase]. | enzyme capable of catalyzing the phosphorylation of thymidine and uridine was isolated from y. pseudotuberculosis cells by fractionation with the use of ammonium sulfate, ion exchange and affinity chromatography. the degree of purification of thymidine- and uridine-kinase was approximately 350 times, and at all stages of isolation the activity of both nucleoside-kinases was detected in the same peaks. the purified enzyme was capable of the phosphorylation of thymidine and uridine at temperatures ... | 2005 | 15881947 |
| [mastitis caused by yersinia pseudotuberculosis in a cow]. | subclinical mastitis with a raised somatic cell count was diagnosed in a cow in her fifth lactation. it was caused by yersinia pseudotuberculosis, which can also infect humans. this is the first time that yersinia pseudotuberculosis has been isolated from a mastitis sample in the netherlands. despite treatment with antibiotics in the dry period, yersinia pseudotuberculosis was still present in the same quarter in the subsequent lactation. the somatic cell count was still high and milk production ... | 2005 | 15938449 |
| oral immunization with a dam mutant of yersinia pseudotuberculosis protects against plague. | inactivation of the gene encoding dna adenine methylase (dam) has been shown to attenuate some pathogens such as salmonella enterica serovar typhimurium and is a lethal mutation in others such as yersinia pseudotuberculosis strain ypiii. in this study the dam methylase gene in yersinia pseudotuberculosis strain ip32953 was inactivated. unlike the wild-type, dna isolated from the mutant could be digested with mboi, which is consistent with an altered pattern of dna methylation. the mutant was sen ... | 2005 | 15941999 |
| inhibition of mapk and nf-kappa b pathways is necessary for rapid apoptosis in macrophages infected with yersinia. | macrophages respond to infection with pathogenic yersinia species by activating mapk- and nf-kappab-signaling pathways. to counteract this response, yersiniae secrete a protease (yersinia outer protein j (yopj)) that is delivered into macrophages, deactivates mapk- and nf-kappab-signaling pathways, and induces apoptosis. nf-kappab promotes cell survival by up-regulating expression of several apoptosis inhibitor genes. previous studies show that deactivation of the nf-kappab pathway by yopj is im ... | 2005 | 15944300 |
| [experimental study on the effect of low molecular dna from salmon milt in pseudotuberculosis infection]. | the effect of low molecular dna from salmon milt (ndna) in experimental pseudotuberculosis in mice was studied. when ndna was admiministered orally, dissemination of the organs by yersinia pseudotuberculosis lowered and the survival of the animals infected with 100-percent lethal dose of the bacteria increased. ndna decreased contamination of the epithelial cells by the microbe in vitro and prevented the lethal effect of the y. pseudotuberculosis toxins on the mice. | 2004 | 15945544 |
| induction of cd8+ t cell responses by yersinia vaccine carrier strains. | yersinia enterocolitica employs a type iii secretion system (ttss) to target virulence factors (e.g. yope) into the cytosol of the host cells. we utilized the ttss to introduce a recombinant antigen directly into the cytosol of host cells and to investigate the potential of y. enterocolitica and y. pseudotuberculosis as live carrier for vaccines. the model antigen ovalbumin (ova) was fused to defined secretion or translocation domains of the yersinia effector protein yope and introduced into att ... | 2005 | 15985316 |
| identification of different regions among strains of yersinia pestis by suppression subtractive hybridization. | yersinia pestis, the causative agent of bubonic and pneumonic plague, has been classified into four biovars: antiqua, mediaevalis, orientalis and microtus. although the entire genome sequences of three y. pestis strains, co92, kim and 91001, of biovar orientalis, mediaevalis and microtus, respectively, have been decoded, the genome sequence of the biovar antiqua strain is unknown. in an initial effort to find antiqua-specific sequences, suppression subtractive hybridization (ssh) was performed a ... | 2005 | 15993571 |
| the comparative importance of e. coli antigen in granulomatous colitis of boxer dogs. | granulomatous colitis of boxer dogs is characterized by mucosal and submucosal infiltration by abundant large macrophages and lymphocytes and plasma cells. involved intestine is thickened, corrugated and ulcerated. the macrophages that occur in colon, cecum and regional lymph nodes are pas-positive, lipid-rich, contain cholesterol, and some of the time can be seen to hold bacteria. paraffin tissue blocks of formalin-fixed colon and colic lymph nodes from 10 cases were cut at 5 microm and immunos ... | 2005 | 15996159 |
| yersinia pseudotuberculosis septicemia and hiv. | two cases of community-acquired septicemia caused by serotype-o1 yersinia pseudotuberculosis were diagnosed in middle-aged, hiv-positive, immunodeficient patients during an 8-month period. bacterial isolates were genetically indistinguishable, but no epidemiologic link between the 2 patients was established. hiv-related immunosuppression should be regarded as a risk factor for y. pseudotuberculosis septicemia. | 2005 | 16022797 |
| yersiniosis in deer from the otago-southland region of new zealand. | samples from 350 routine deer cases submitted to invermay animal health laboratory for diagnosis during 1979-1982 were examined specifically for the presence of yersinia sp. an analysis of 57 cases of yersiniosis due to yersinia pseudotuberculosis is made and two cases due to yersinia enterocolitica are described. the occurrence of cases appeared strongly correlated with periods of stress, predominantly in winter when cool wet conditions and lack of grazing combined to precipitate the disease. a ... | 1983 | 16030939 |
| faecal survey of deer for yersinia pseudotuberculosis and salmonella sp. | the results of a national survey of faeces from clinically normal deer for salmonella sp. and yersinia pseudotuberculosis are reported. five isolates of y. pseudotuberculosis and none of salmonella sp. were made from 3810 faeces representing 122 farms. | 1983 | 16030940 |
| serotypes of yersinia pseudotuberculosis recovered from domestic livestock. | the serological identity of 234 strains of yersinia pseudotuberculosis recovered from domestic animals and birds in new zealand was determined by slide agglutination test. thirty strains were also examined by tube agglutination test. the strains were isolated from cattle (56), sheep (8), deer (117), goats (13), pigs (7), rabbits (6), guinea pigs (5), and aviary species of birds (22). all strains were isolated from animals or birds which had died or shown signs of ill health and amongst which dia ... | 1984 | 16031006 |
| potential wildlife sources of yersinia pseudotuberculosis for farmed deer (cervus elaphus). | during 1982 and 1983 15 serotype i, 6 serotype ii, 1 serotype iii and 3 untyped strains of yersinia pseudotuberculosis were isolated from 675 apparently normal small mammals and birds from the invermay farm and nearby rubbish tip with the following prevalence rates: feral cats 27.8%, norway rats 8.6%, mice 5.5%, hares 3.8% rabbits 1.9% ducks 5.3%, sparrows 2.3%, seagulls 2.3% and starlings 1.7%. for rabbits a significantly higher prevalence of infection was found in the autumn/winter period (4.8 ... | 1984 | 16031024 |
| yersinia pseudotuberculosis recovered from the faeces of clinically healthy deer. | 1984 | 16031054 | |
| recovery of yersinia pseudotuberculosis from the faeces of healthy cattle. | attempts were made to recover and serologically identify yersinia pseudotuberculosis from the faeces of groups of nine to twenty clinically healthy cattle eight to thirteen months old on each of 50 farms in the northern part of new zealand. yersinia pseudotuberculosis was recovered from 134 (26.3%) of 509 faeces samples from cattle on 42 (84%) farms and from nine of ten samples on two of these farms. serotypes i, ii, and iii were identified, of which serotype iii was by far the most common and a ... | 1985 | 16031106 |
| growth of yersinia pseudotuberculosis on selective media. | 1994 | 16031741 | |
| attempted definition by immunoblotting of the causes of reactivity in suspected false-positive sera in the brucella ovis complement fixation test. | seventy-nine suspected false-positive sera, obtained over 1 year from routine submissions for brucella ovis serological testing, were used in this study. these sera, which exhibited titres in the complement fixation test, but which because of their epidemiological history and their reactions in the enzyme-linked immunosorbent assay and gel diffusion test were suspected to be false positives, were further analysed by immunoblotting. in blots, using b. ovis antigens, rough lipopolysaccharide was i ... | 1996 | 16031926 |
| the nematode panagrellus redivivus is susceptible to killing by human pathogens at 37 degrees c. | caenorhabditis elegans has been used as a host for the study of bacteria that cause disease in mammals. however, a significant limitation of the model is that c. elegans is not viable at 37 degrees c. we report that the gonochoristic nematode panagrellus redivivus survives at 37 degrees c and maintains its life cycle at temperatures up to and including 31.5 degrees c. the c. elegans pathogens pseudomonas aeruginosa, salmonella enterica, staphylococcus aureus, but not yersinia pseudotuberculosis, ... | 2005 | 16040202 |
| influence of na(+), dicarboxylic amino acids, and ph in modulating the low-calcium response of yersinia pestis. | the virulence of yersiniae is promoted in part by shared approximately 70-kb plasmids (pcd in yersinia pestis and pyv in enteropathogenic yersinia pseudotuberculosis and yersinia enterocolitica) that mediate a low-calcium response. this phenotype is characterized at 37 degrees c by either bacteriostasis in ca(2+)-deficient medium with expression of pcd/pyv-encoded virulence effectors (yops and lcrv) or vegetative growth and repression of yops and lcrv with > or =2.5 mm ca(2+) (lcr(+)). regulatio ... | 2005 | 16040987 |
| horizontal transfer of yersinia high-pathogenicity island by the conjugative rp4 attb target-presenting shuttle plasmid. | the high-pathogenicity island (hpi) encodes a highly efficient yersiniabactin system of iron acquisition responsible for mouse lethality in yersinia. although the hpi is widely disseminated among enterobacteriaceae it lacks functions necessary for its replication and transmission. therefore, the mechanism of its horizontal transfer and circulation is completely obscure. on the other hand, the hpi is a genetically active island in the bacterial cell. it encodes a functional recombinase and is abl ... | 2005 | 16045617 |
| the evolution of flea-borne transmission in yersinia pestis. | transmission by fleabite is a recent evolutionary adaptation that distinguishes yersinia pestis, the agent of plague, from yersinia pseudotuberculosis and all other enteric bacteria. the very close genetic relationship between y. pestis and y. pseudotuberculosis indicates that just a few discrete genetic changes were sufficient to give rise to flea-borne transmission. y. pestis exhibits a distinct infection phenotype in its flea vector, and a transmissible infection depends on genes that are spe ... | 2005 | 16053250 |
| homology models of the yersinia pseudotuberculosis and yersinia pestis general porins and comparative analysis of their functional and antigenic regions. | the amino acid sequences of the yersinia pseudotuberculosis porin (yps) and y. pestis porin (ypt) have recently deduced but their three-dimensional structures were not known. these sequences were analyzed using the servers 3d-pssm and predport. the yps and ypt porins were shown to have a high degree of identity (above 50%) in primary and secondary structures. the three-dimensional models of the yersinia pseudotuberculosis porin (yps) and y. pestis porin (ypt) were obtained using the homology mod ... | 2005 | 16060690 |
| real-time pcr assays targeting a unique chromosomal sequence of yersinia pestis. | yersinia pestis, the causative agent of the zoonotic infection plague, is a major concern as a potential bioweapon. current real-time pcr assays used for y. pestis detection are based on plasmid targets, some of which may generate false-positive results. | 2005 | 16099940 |
| identification and expression of a host-recognized antigen, fspa, from flavobacterium psychrophilum. | flavobacterium psychrophilum is the aetiological agent of rainbow trout fry syndrome, an economically important disease of immature salmonid fish for which there is no vaccine. convalescent serum from the host, rainbow trout (oncorhynchus mykiss), reacted strongly with a approximately 20 kda, flavobacterium-specific protein antigen (subsequently named fspa) from f. psychrophilum. protein-enriched, detergent-partitioned samples were separated by two-dimensional gel electrophoresis and the protein ... | 2005 | 16151223 |
| distinct mechanisms of integrin binding by yersinia pseudotuberculosis adhesins determine the phagocytic response of host macrophages. | the enteropathogenic yersiniae express two outer membrane adhesins, invasin and yada, that contribute to pathogenesis. while invasin binds directly to beta1 integrin receptors with high affinity, yada binds indirectly through extracellular matrix (ecm) components. in this study, yersinia pseudotuberculosis inv and yada mutants were used to investigate how these distinct binding mechanisms compare and potentially compete in activating signalling pathways and promoting bacterial uptake by host mac ... | 2005 | 16153246 |
| a cohort study of yersinia infection in goats. | to determine the temporal pattern of yersinia infections in three goat flocks and examine the influence of management and seasonal factors on the incidence of those infections over a 1-year period. | 2005 | 16167386 |
| forming and immunological properties of some lipopolysaccharide-chitosan complexes. | the complex formation of lipopolysaccharide (lps) with chitosan (ch) was demonstrated using sedimentation velocity analysis in the analytical ultracentrifuge, centrifugation in glycerol gradient and isopicnic centrifugation in cesium chloride. an addition of ch to the escherichia coli and yersinia pseudotuberculosis lps solutions was found to result in formation of the stable lps-ch complexes. the interaction is a complicated process and depends on time and reaction temperature, as well as on th ... | 2006 | 16181724 |
| caprine enteritis associated with yersinia pseudotuberculosis infection. | yersiniosis was prevalent among a caprine herd during the late autumn of 2003 in iwate prefecture, japan. the disease affected 29 of about 100 lactating goats, but not dried or nonparous goats, mature male goats or kids. four animals died within an epidemic period of 20 days. affected animals developed decreased milk production with subsequent watery diarrhea, neutrophilia with increased band forms and multiple microabscesses characteristic of yersiniosis in the intestinal mucosa from the jejunu ... | 2005 | 16210800 |
| yersinia pseudotuberculosis spatially controls activation and misregulation of host cell rac1. | yersinia pseudotuberculosis binds host cells and modulates the mammalian rac1 guanosine triphosphatase (gtpase) at two levels. activation of rac1 results from integrin receptor engagement, while misregulation is promoted by translocation of yope and yopt proteins into target cells. little is known regarding how these various factors interplay to control rac1 dynamics. to investigate these competing processes, the localization of rac1 activation was imaged microscopically using fluorescence reson ... | 2005 | 16228016 |
| evolutionary population genetics of promoters: predicting binding sites and functional phylogenies. | we study the evolution of transcription factor-binding sites in prokaryotes, using an empirically grounded model with point mutations and genetic drift. selection acts on the site sequence via its binding affinity to the corresponding transcription factor. calibrating the model with populations of functional binding sites, we verify this form of selection and show that typical sites are under substantial selection pressure for functionality: for camp response protein sites in escherichia coli, t ... | 2005 | 16236723 |
| a multiplex polymerase chain reaction microarray assay to detect bioterror pathogens in blood. | heightened concern about the dangers of bioterrorism requires that measures be developed to ensure the safety of the blood supply. multiplex detection of such agents using a blood-screening dna microarray is a sensitive and specific method to screen simultaneously for a number of suspected agents. we have developed and optimized a multiplex polymerase chain reaction microarray assay to screen blood for three potential bioterror bacterial pathogens and a human ribosomal rna gene internal control. ... | 2005 | 16237218 |
| identification of gmha, a yersinia pestis gene required for flea blockage, by using a caenorhabditis elegans biofilm system. | yersinia pestis, the cause of bubonic plague, blocks feeding by its vector, the flea. recent evidence indicates that blockage is mediated by an in vivo biofilm. y. pestis and the closely related yersinia pseudotuberculosis also make biofilms on the cuticle of the nematode caenorhabditis elegans, which block this laboratory animal's feeding. random screening of y. pseudotuberculosis transposon insertion mutants with a c. elegans biofilm assay identified gmha as a gene required for normal biofilms ... | 2005 | 16239518 |
| analysis of rova, a transcriptional regulator of yersinia pseudotuberculosis virulence that acts through antirepression and direct transcriptional activation. | the transcription factor rova of yersinia pseudotuberculosis and analogous proteins in other enterobacteriaceae activate the expression of virulence genes that play a crucial role in stress adaptation and pathogenesis. in this study, we demonstrate that the rova protein forms dimers independent of dna binding, stimulates rna polymerase, most likely via its c-terminal domain, and counteracts transcriptional repression by the histone-like protein h-ns. as the molecular function of the rova family ... | 2005 | 16257976 |
| mapping of a yscy binding domain within the lcrh chaperone that is required for regulation of yersinia type iii secretion. | type iii secretion systems are used by many animal and plant interacting bacteria to colonize their host. these systems are often composed of at least 40 genes, making their temporal and spatial regulation very complex. some type iii chaperones of the translocator class are important regulatory molecules, such as the lcrh chaperone of yersinia pseudotuberculosis. in contrast, the highly homologous pcrh chaperone has no regulatory effect in native pseudomonas aeruginosa or when produced in yersin ... | 2005 | 16267298 |
| molecular characteristics of ompf-like porins from pathogenic yersinia. | nonspecific pore-forming proteins (porins) are the major proteins of the outer membrane of gram-negative bacteria responsible for diffusion of low-molecular-weight compounds. nucleotide sequences of the ompf-like porins from the pathogenic bacteria yersinia pseudotuberculosis (yps) and yersinia enterocolitica (ye) were cloned and determined. values of molecular weights (mw) and isoelectric points (iep) calculated for these proteins (for ompf-yps: mw 37.7 kd, iep 4.45; for ompf-ye: mw 39.5 kd, ie ... | 2005 | 16271025 |
| evaluation of o-antigen inactivation on pla activity and virulence of yersinia pseudotuberculosis harbouring the ppla plasmid. | yersinia pestis is a species that emerged recently from yersinia pseudotuberculosis and gained an exceptional pathogenicity potential. among the major genetic differences between the plague bacillus and its ancestor is the acquisition of the ppla plasmid, which has been associated with the increased virulence of y. pestis. in a previous study, introduction of ppla into y. pseudotuberculosis did not lead to any modification of the virulence of the host bacterium. however, it was subsequently demo ... | 2005 | 16272397 |
| evaluation of a western blot method for the detection of yersinia antibodies: evidence of serological cross-reactivity between yersinia outer membrane proteins and borrelia burgdorferi. | yersinia enterocolitica and yersinia pseudotuberculosis have been identified as causative organisms of reactive arthritis in humans. we evaluated a western blot assay which uses yersinia outer membrane proteins as antigens for the detection of yersinia antibodies as a replacement for the complement fixation (cf) assay. clinical agreement, sensitivity, and specificity were determined by testing 19 positive and 21 negative serum samples by the cf assay, western blot assay, and enzyme-linked immuno ... | 2005 | 16275939 |
| [influence of glucose and galactose on the morphology and biological properties of yersinia pseudotuberculosis]. | when cultivated in the presence of glucose, irrespective of temperature and the degree of aeration, y. pseudotuberculosis cells have the ovoid form, constant size and low hydrophobic properties of their surface. meanwhile the characteristics of the bacteria grown in the medium, carbohydrate-free or with galactose added, essentially depend on the conditions of medium aeration. under the conditions of intensive stirring at both temperatures these bacteria acquire the coccoid form, not typical for ... | 2005 | 16279525 |
| conserved role of the linker alpha-helix of the bacterial disulfide isomerase dsbc in the avoidance of misoxidation by dsbb. | in the bacterial periplasm the co-existence of a catalyst of disulfide bond formation (dsba) that is maintained in an oxidized state and of a reduced enzyme that catalyzes the rearrangement of mispaired cysteine residues (dsbc) is important for the folding of proteins containing multiple disulfide bonds. the kinetic partitioning of the dsba/dsbb and dsbc/dsbd pathways partly depends on the ability of dsbb to oxidize dsba at rates >1000 times greater than dsbc. we show that the resistance of dsbc ... | 2006 | 16280324 |
| yersinia yop-specific iga antibodies in hungarian blood donors. | sera of 112 healthy hungarian blood donors were tested for the presence of yersinia enterocolitica and y. pseudotuberculosis-specific agglutinins by tube agglutination, and for that of yersinia outer membrane protein (yop)-specific iga antibodies by elisa. the positive results of this latter assay were confirmed by immunoblot. only one sample gave a positive agglutination reaction with y. enterocolitica antigen (group 03) and four exhibited an equivocal reaction with y. pseudotuberculosis antige ... | 2005 | 16295667 |
| clpv, a unique hsp100/clp member of pathogenic proteobacteria. | hsp100/clp proteins are key players in the protein quality control network of prokaryotic cells and function in the degradation and refolding of misfolded or aggregated proteins. here we report the identification of a new class of hsp100/clp proteins, termed clpv (virulent strain), that are present in bacteria interacting with eukaryotic cells, including human pathogens. the clpv proteins are most similar to clpb proteins within the hsp100/clp family, but cluster in a separate phylogenetic tree ... | 2005 | 16307477 |
| environmentally controlled invasion of cancer cells by engineered bacteria. | bacteria can sense their environment, distinguish between cell types, and deliver proteins to eukaryotic cells. here, we engineer the interaction between bacteria and cancer cells to depend on heterologous environmental signals. we have characterized invasin from yersinia pseudotuburculosis as an output module that enables escherichia coli to invade cancer-derived cells, including hela, hepg2, and u2os lines. to environmentally restrict invasion, we placed this module under the control of hetero ... | 2006 | 16330045 |
| evaluation of ribotyping as a tool for molecular typing of yersinia pseudotuberculosis strains of worldwide origin. | yersinia pseudotuberculosis is a gram-negative bacterium that infects a wide range of animals, including humans, and is transmitted by the fecal-oral route. this species is found globally and is responsible for human outbreaks, mainly in cold countries. the aim of this study was to evaluate the potential of ribotyping for the molecular typing of worldwide isolates. for this purpose, 80 strains of y. pseudotuberculosis belonging to the six classical serotypes and nine subserotypes and isolated fr ... | 2005 | 16333119 |
| yersiniophage phir1-37 is a tailed bacteriophage having a 270 kb dna genome with thymidine replaced by deoxyuridine. | bacteriophage pir1-37 was isolated based on its ability to infect strain yeo3-r1, a virulence-plasmid-cured o antigen-negative derivative of yersinia enterocolitica serotype o : 3. in this study, the phage receptor was found to be a structure in the outer core hexasaccharide of y. enterocolitica o : 3 lps. the phage receptor was present in the outer core of strains of many other y. enterocolitica serotypes, but also in some yersinia intermedia strains. surprisingly, the receptor structure reside ... | 2005 | 16339954 |
| [yersinia pseudotuberculosis type 4a infection meeting the diagnostic criteria for kawasaki disease complicated by disseminated intravascular coagulation]. | we report a case of yersinia pseudotuberculosis (y. ptbc) infection complicated by disseminated intravascular coagulation (dic) that presented as kawasaki disease (kd). a 9-year-old girl had been well until two days before, when she developed a fever, exanthem, and abdominal pain. an erythematous macular rash was observed in the perineum, and she had a strawberry tongue. the patient was admitted to kawasaki medical school hospital because the macular rash spread over her entire body, and edema o ... | 2005 | 16366361 |
| serotype differences and lack of biofilm formation characterize yersinia pseudotuberculosis infection of the xenopsylla cheopis flea vector of yersinia pestis. | yersinia pestis, the agent of plague, is usually transmitted by fleas. to produce a transmissible infection, y. pestis colonizes the flea midgut and forms a biofilm in the proventricular valve, which blocks normal blood feeding. the enteropathogen yersinia pseudotuberculosis, from which y. pestis recently evolved, is not transmitted by fleas. however, both y. pestis and y. pseudotuberculosis form biofilms that adhere to the external mouthparts and block feeding of caenorhabditis elegans nematode ... | 2006 | 16428415 |
| a role for the smpb-ssra system in yersinia pseudotuberculosis pathogenesis. | yersinia utilizes a sophisticated type iii secretion system to enhance its chances of survival and to overcome the host immune system. smpb (small protein b) and ssra (small stable rna a) are components of a unique bacterial translational control system that help maintain the bacterial translational machinery in a fully operational state. we have found that loss of the smpb-ssra function causes acute defects in the ability of yersinia pseudotuberculosis to survive in hostile environments. most s ... | 2006 | 16450010 |
| seroprevalence of anti-yersinia antibodies in healthy austrians. | yersiniosis is caused by y. enterocolitica and y. pseudotuberculosis mostly presenting as intestinal infection. the infection is usually acquired from contaminated food. the aim of this study was to determine the seroprevalence of anti-yersinia antibodies in austrians. sera of 750 healthy austrians from all nine states were tested for anti-yersinia igg antibodies using the recomblot yersinia westernblot kit. overall seroprevalence was 29.7%. seroprevalence increased significantly with age from 2 ... | 2006 | 16450210 |
| quorum sensing and the lifestyle of yersinia. | bacterial cell-to-cell communication ('quorum sensing') is mediated by structurally diverse, small diffusible signal molecules which regulate gene expression as a function of cell population density. many different gram-negative animal, plant and fish pathogens employ n-acylhomoserine lactones (ahls) as quorum sensing signal molecules which control diverse physiological processes including bioluminescence, swarming, antibiotic biosynthesis, plasmid conjugal transfer, biofilm development and viru ... | 2006 | 16450882 |
| identification of a domain in yersinia virulence factor yada that is crucial for extracellular matrix-specific cell adhesion and uptake. | for many pathogens, cell adhesion factors are critical virulence determinants. enteropathogenic yersinia species express the afimbrial adhesin yada, the prototype of a class of homotrimeric outer membrane adhesins, which mediates adherence to host cells by binding to extracellular matrix components. in this study, we demonstrate that different pathogenic functions are attributable to highly homologous yada proteins. yada of yersinia pseudotuberculosis (yada(pstb)) and yersinia enterocolitica (ya ... | 2006 | 16488979 |
| the proinflammatory response induced by wild-type yersinia pseudotuberculosis infection inhibits survival of yop mutants in the gastrointestinal tract and peyer's patches. | single-strain infections and coinfections are frequently used to assess roles of virulence factors in infected tissues. after oral inoculation of mice, yersinia pseudotuberculosis yope and yoph mutants colonize the intestines and peyer's patches in single-strain infections but fail to persist in competition with wild-type y. pseudotuberculosis, indicating that these two infection models provide different insights into the roles of yops. to determine how wild-type y. pseudotuberculosis hinders yo ... | 2006 | 16495522 |
| endoproteolytic processing of rhoa by rce1 is required for the cleavage of rhoa by yersinia enterocolitica outer protein t. | the bacterial toxin yersinia outer protein t (yopt) is a cysteine protease that cleaves rho gtpases immediately upstream of a carboxyl-terminal isoprenylcysteine. by clipping off the lipid anchor, yopt releases rho gtpases from membranes, resulting in rounding up of mammalian cells in culture. the proteolytic activity of yopt depends on the isoprenylation of the cysteine within the carboxyl-terminal caax motif, a reaction carried out by geranylgeranyltransferase type i. the caax motif (where "a" ... | 2006 | 16495543 |
| the twin arginine translocation system is essential for virulence of yersinia pseudotuberculosis. | yersinia species pathogenic to humans have been extensively characterized with respect to type iii secretion and its essential role in virulence. this study concerns the twin arginine translocation (tat) pathway utilized by gram-negative bacteria to secrete folded proteins across the bacterial inner membrane into the periplasmic compartment. we have shown that the yersinia tat system is functional and required for motility and contributes to acid resistance. a yersinia pseudotuberculosis mutant ... | 2006 | 16495550 |
| biosynthesis of a 3,6-dideoxyhexose: crystallization and x-ray diffraction of cdp-6-deoxy-l-threo-d-glycero-4-hexulose-3-dehydrase (e1) for ascarylose biosynthesis. | cdp-6-deoxy-l-threo-d-glycero-4-hexulose-3-dehydrase (e1), along with its reductase (e3), catalyzes the unusual c-3 deoxygenation of cdp-6-deoxy-l-threo-d-glycero-4-hexulose to form cdp-3,6-dideoxy-l-threo-d-glycero-4-hexulose in cdp-ascarylose biosynthesis [chen et al. (1996), biochemistry, 35, 16412-16420]. this dimeric [2fe-2s] protein, cloned from the bacteria yersinia pseudotuberculosis, is currently the only known example of an enzyme that uses a vitamin b6-derived pyridoxamine 5'-phosphat ... | 2006 | 16511309 |
| veterinary and medical aspects of abortion in danish sheep. | the danish sheep population totals around 144,000 animals, but little is known of the causes and prevalance of diseases. this study focuses on the causes of abortion in danish sheep. during one breeding season, aborted foetuses and stillbirths with signs of intrauterine death or malformation were submitted for laboratory examination from a population of 3,758 breeding ewes. samples from 24 incidents of abortion and 21 ewes delivering malformed lambs or lambs with ante partum decomposition were s ... | 2006 | 16519752 |
| [inhibition of prokaryote and eukaryote cells adhesion by sea proteobacteria pseudoalteromonas nigrifaciens kmm 156 lipopolysaccharide and its fragments]. | the impact of pseudoalteromonas nigrifaciens kmm 156 lipopolysaccharide and its fragments on adhesion of prokaryote and eukaryote cells was studied. the lipopolysaccharide and o-specific polysaccharide identical by its structure to capsular polysaccharide lowered the number of the yersinia pseudotuberculosis bacteria attached to sheep erythrocytes and adhesion of mouse neutrophils to plastic. the kora oligosaccharide had no effect on the processes. | 2005 | 16526601 |
| [experimental morphological study of the therapeutic effect of shark cartilage preparation in a model of infective allergic arthritis]. | the therapeutic effect of a polar shark cartilage preparation which is an enzymatic hydrolysate was studied in a rabbit model of infective allergic pseudotuberculous arthritis. characterization of the chemical composition of the preparation designed by an original method is presented. improvement of the general state of the affected joints and development of tissue immunomorphological responses were shown. | 2005 | 16526605 |
| determination of binding constants of lipopolysaccharides of different structure with chitosan. | the interaction of endotoxins--lipopolysaccharides (lps) different in degree of the o-specific chain polymerization--with 20- and 130-kd chitosan was studied using the competitive binding of lps with the complex of chitosan-anionic dye (tropaeolin 000-2) and the direct binding of (125)i-labeled lps with chitosan immobilized on sepharose 4b. the interaction of 20-kd chitosan with lps was non-cooperative, and immobilization of the polycation on sepharose resulted in its binding to (125)i-labeled l ... | 2006 | 16545072 |
| intracellular pasteurella pseudotuberculosis: multiplication in cultured spleen and kidney cells. | pasteurella pseudotuberculosis multiplied within rabbit cells in primary culture. spleen cells from rabbits were either dispersed mechanically, exposed to the organism immediately after cell preparation, and grown as a pellet or the cells were dispersed enzymatically and grown as a monolayer for 4 to 6 days before the bacteria were introduced. intracellular multiplication proceeded at a logarithmic rate for 1 to 2 days, with a generation time of about 70 min in pelleted cells and 4 to 5 hr in mo ... | 1970 | 16557888 |
| the endotoxin of pasteurella pseudotuberculosis. | 1948 | 16561559 | |
| glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities of pasteurella pestis and pasteurella pseudotuberculosis. | 1962 | 16561972 | |
| recombinant escherichia coli expressing invasin targets the peyer's patches: the basis for a bacterial formulation for oral vaccination. | we have investigated the tropism of nonpathogenic recombinant invasive escherichia coli in the gastrointestinal tract and the efficacy of this invasive e. coli as an oral vaccine for cancer immunotherapy. e. coli expressing invasin from yersinia pseudotuberculosis selectively invade nonphagocytic cells in which beta(1)-integrin is expressed and accessible. following internalization the e. coli are degraded in the phagosome. coexpression of listeriolysin o (llo) mediates release of the content of ... | 2006 | 16581299 |
| exoenzyme s of pseudomonas aeruginosa is not able to induce apoptosis when cells express activated proteins, such as ras or protein kinase b/akt. | intracellular targeting of the pseudomonas aeruginosa toxins, such as exoenzyme s (exos), cause cell death, as well as morphological and physiological changes in various tissue culture cells and animal models. in this report we have investigated the mechanism behind exos-mediated cell death. in order to address this issue, we have used cell lines expressing activated forms of various components of the ras signalling pathway in order to evaluate the importance of the ras pathway for viability and ... | 2006 | 16611230 |
| effect of temperature on synthesis of polyphosphates in yersinia pseudotuberculosis and listeria monocytogenes under starvation conditions. | it was found that at low temperature (6-8 degrees c) in the absence of nitrogen supply and at the presence of phosphate ions in the medium, yersinia pseudotuberculosis and listeria monocytogenes are able to actively synthesize reserve substances as polyphosphates. most of the bacterial polyphosphates are alkali-soluble, especially at the preliminary stage of cell growth (lag-phase). this is proved by electron microscopic studies of ultrastructure of model microorganisms. during a long starvation ... | 2006 | 16615864 |
| the ser/thr kinase activity of the yersinia protein kinase a (ypka) is necessary for full virulence in the mouse, mollifying phagocytes, and disrupting the eukaryotic cytoskeleton. | the yersinia protein kinase a (ypka) is injected into host cells by the yersinial type three secretion system (ttss). ypka is widely believed to function within the host cell based on the fact that its kinase domain is clearly homologous to eukaryotic ser/thr kinases and that its enzymatic activity, when assayed in vitro, is dependent on eukaryotic-derived host factors. whether this activity is required for virulence has not been addressed. here, we report that a yersinia pseudotuberculosis stra ... | 2006 | 16626927 |
| direct visualization of red fluorescent lipoproteins indicates conservation of the membrane sorting rules in the family enterobacteriaceae. | chimeras created by fusing the monomeric red fluorescent protein (rfp) to a bacterial lipoprotein signal peptide (liporfps) were visualized in the cell envelope by epifluorescence microscopy. plasmolysis of the bacteria separated the inner and outer membranes, allowing the specific subcellular localization of liporfps to be determined in situ. when equipped with the canonical inner membrane lipoprotein retention signal cdsr, liporfp was located in the inner membrane in escherichia coli, whereas ... | 2006 | 16672606 |
| functional analysis of the yope gtpase-activating protein (gap) activity of yersinia pseudotuberculosis. | yope of yersinia pseudotuberculosis inactivates three members of the small rhogtpase family (rhoa, rac1 and cdc42) in vitro and mutation of a critical arginine abolishes both in vitro gtpase-activating protein (gap) activity and cytotoxicity towards hela cells, and renders the pathogen avirulent in a mouse model. to understand the functional role of yope, in vivo studies of the gap activity in infected eukaryotic cells were conducted. wild-type yope inactivated rac1 as early as 5 min after infec ... | 2006 | 16681842 |
| immunomodulatory properties of cumaside. | the medical lead, so-called cumaside, was created on the basis of triterpene oligoglycosides from the far-eastern edible sea cucumber (holothurian) cucumaria japonica and its immunomodulatory properties were studied. the haemolytic activity of cumaside was significantly reduced in comparison with original glycosides due to the glycoside-cholesterol complex formation. the influence of cumaside on mouse macrophages in low doses was accompanied by more then two-fold stimulation of lysosomal activit ... | 2006 | 16714210 |
| complete genome sequence of yersinia pestis strains antiqua and nepal516: evidence of gene reduction in an emerging pathogen. | yersinia pestis, the causative agent of bubonic and pneumonic plagues, has undergone detailed study at the molecular level. to further investigate the genomic diversity among this group and to help characterize lineages of the plague organism that have no sequenced members, we present here the genomes of two isolates of the "classical" antiqua biovar, strains antiqua and nepal516. the genomes of antiqua and nepal516 are 4.7 mb and 4.5 mb and encode 4,138 and 3,956 open reading frames, respective ... | 2006 | 16740952 |
| yersinia enterocolitica type iii secretion chaperone sycd: recombinant expression, purification and characterization of a homodimer. | yersinia species pathogenic to human benefit from a protein transport machinery, a type three secretion system (t3ss), which enables the bacteria to inject effector proteins into host cells. several of the transport substrates of the yersinia t3ss, called yops (yersinia outer proteins), are assisted by specific chaperones (syc for specific yop chaperone) prior to transport. yersinia enterocolitica sycd (lcrh in yersinia pestis and yersinia pseudotuberculosis) is a chaperone dedicated to the assi ... | 2006 | 16750393 |
| independent acquisition of site-specific recombination factors by asn trna gene-targeting genomic islands. | two genomic islands, namely the high-pathogenicity island (hpi) and ecoc54n target the same asn trna genes to integrate into the bacterial chromosome. the hpi encodes the siderophore yersiniabactin in the highly pathogenic yersinia group (yersinia pestis, yersinia pseudotuberculosis and yersinia enterocolitica 1b) whilst the ecoc54n island possibly encodes a polyketide synthase with an unknown function in the uropathogenic escherichia coli cft073 strain. hpi encodes the recombinase that promotes ... | 2006 | 16753337 |
| yersinia pseudotuberculosis disseminates directly from a replicating bacterial pool in the intestine. | dissemination of yersinia pseudotuberculosis within mice after oral inoculation was analyzed. y. pseudotuberculosis translocated to organs such as the liver and spleen shortly after oral inoculation, but was quickly cleared. in contrast, a second temporally distinct bacterial translocation event resulted in successful hepatosplenic replication of the bacteria. replicating pools of bacteria could be established in these organs in mouse mutants that lacked peyer's patches. these animals frequently ... | 2006 | 16754724 |
| [anthropurgic foci of pseudotuberculosis and the mechanisms of their formation in groups of servicemen]. | at the period of 1982 - 2003, morbidity rate in pseudotuberculosis and the yersinia pseudotuberculosis culture rates in groups of servicemen (from different abiotic objects, from humans and small rodents) in different geographic zones were studied. the cases of the isolation of y. pseudotuberculosis were most frequently registered in groups of servicemen in the far east and the kola peninsula. in these regions the highest morbidity rate in pseudotuberculosis was registered among servicemen. the ... | 2006 | 16758891 |
| [development of an immunochromayography assay method for the detection of yersinia pestis]. | to develop a method of immunochromatography assay (ica) with sensitive, specific, rapid, simple and suitable for the detection of yersinia pestis antigen at the local laboratories. | 2006 | 16792900 |
| mesenteric adenitis caused by yersinia pseudotubercolosis in a patient subsequently diagnosed with crohn's disease of the terminal ileum. | although the association between inflammatory bowel disease and gastrointestinal infections has been suggested, the mechanisms involved in the pathogenesis of crohn's disease (cd) are still undetermined. we report the case of a man, who presented with mesenteric adenitis initially due to a yersinia pseudotubercolosis infection, who was later diagnosed with crohn's disease. this case is in keeping with recent evidence in the literature which suggests that cd is a disease linked to abnormal immune ... | 2006 | 16804986 |
| a non-invasive quantitative assay to measure murine intestinal inflammation using the neutrophil marker lactoferrin. | intestinal inflammation in mice is most frequently assessed by histology or facs, processes that necessitate sacrificing mice. we developed a lactoferrin elisa for murine feces to quantify intestinal inflammation in mice with enteric infections or colitis. levels of fecal lactoferrin, a protein secreted by activated neutrophils, were consistent with neutrophil infiltration as assessed by histology, indicating that this fecal lactoferrin elisa is a good alternative to histology. the fecal lactofe ... | 2006 | 16806255 |