Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| feasibility of providing sexually transmitted infection testing and treatment in off-campus, nonclinic settings for adolescents enrolled in a school-based research project. | this study examined the acceptability and feasibility of using a biological outcome measure to evaluate a school-based sexuality education program. confidential field-delivered sexually transmitted infection (sti) testing by nonmedical field staff and sti treatment by medically trained field staff was assessed in off-campus and off-clinic settings for adolescents enrolled in the trial. | 2014 | 24749920 |
| the effect of 3-(biphenyl-4-yl)-3-hydoxyquinuclidine (bpq-oh) and metronidazole on trichomonas vaginalis: a comparative study. | trichomonas vaginalis causes trichomoniasis in humans, a sexually transmitted disease commonly treated with metronidazole (mtz), a drug that presents some toxicity, causing undesirable side effects. in addition, an increase in metronidazole-resistant parasites has been reported. thus, the development of alternative treatment is recommended. to date, the search for antiparasitic drugs has been based on different approaches: identification of active natural products, identification of parasite tar ... | 2014 | 24752367 |
| increased susceptibility to vaginal simian/human immunodeficiency virus transmission in pig-tailed macaques coinfected with chlamydia trachomatis and trichomonas vaginalis. | sexually transmitted infections (stis) are associated with an increased risk of human immunodeficiency virus (hiv) infection, but their biological effect on hiv susceptibility is not fully understood. | 2014 | 24755433 |
| mycoplasma genitalium infection is associated with microscopic signs of cervical inflammation in liquid cytology specimens. | cervicitis is a common clinical finding often attributed to sexually transmitted infections (stis), but no etiologic agent is identified in the majority of cases. in this study, we comparatively assessed inflammation among the common infectious etiologies of cervicitis and assessed the potential value of liquid cytology specimens for predicting stis. among 473 louisiana women at low risk for acquiring stis, the prevalences of mycoplasma genitalium, chlamydia trachomatis, neisseria gonorrhoeae, a ... | 2014 | 24759719 |
| university students and the risk of hiv and other sexually transmitted infections in uganda: the crane survey. | adolescents and young adults are at high risk of human immunodeficiency virus (hiv) infection in sub-saharan africa. previous reports have found that university students in africa comprise a sexually active population, although the prevalence of hiv or sexually transmitted infections (sti) has not been measured. we conducted a cross-sectional survey of students from five large universities in kampala, uganda, using respondent-driven sampling. we asked students to complete behavioral questionnair ... | 2014 | 24762640 |
| gonorrhoea, chlamydia, syphilis and trichomonas in children under 13 years of age: national surveillance in the uk and republic of ireland. | sexually transmitted infections in children ought to raise concerns about sexual abuse. it is not known how frequently they are identified in the uk and ireland, nor how well they are investigated. | 2014 | 24771307 |
| development of a loop-mediated isothermal amplification assay for detection of trichomonas vaginalis. | a loop-mediated isothermal amplification (lamp) assay targeting the 2-kbp repeated dna species-specific sequence was developed for detection of trichomonas vaginalis, the causative agent of trichomoniasis. the analytical sensitivity and specificity of the lamp assay were evaluated using pooled genital swab and urine specimens, respectively, spiked with t. vaginalis trophozoites. genital secretion and urine did not inhibit the detection of the parasite. the sensitivity of the lamp was 10-1000 tim ... | 2014 | 24792836 |
| neglected parasitic infections in the united states: trichomoniasis. | trichomonas vaginalis is one of the most common human parasitic infections in the united states, as well as the most prevalent non-viral sexually transmitted infection. however, it has long received much less consideration than other parasitic and sexually transmitted diseases. much of this inattention can be attributed to a poor understanding of the public health impact of trichomoniasis. increasing recognition of the sequelae of infection, including increased risk of infection with human immun ... | 2014 | 24808247 |
| bead-based multiplex sexually transmitted infection profiling. | sexually transmitted infections are a significant cause of genital disease, infertility and hospital admissions. the economic impact is high. an accurate diagnosis is often difficult and time consuming. we report the development and validation of a novel bead-based multiplex sexually transmitted infection profiling (stip) assay that detects 18 sexually transmitted infections using a multiplex pcr followed by luminex bead-based hybridisation. | 2014 | 24814157 |
| trichomonas vaginalis as a cause of perinatal morbidity: a systematic review and meta-analysis. | trichomonas vaginalis is the most common curable sexually transmissible infection worldwide, with high rates in women of reproductive age. there have been inconsistent findings about the impact of infection and its treatment in pregnancy. we conducted a meta-analysis to determine the association between t. vaginalis and perinatal outcomes. electronic databases were searched to may 2013. included studies reported perinatal outcomes in women infected and uninfected with t. vaginalis. meta-analysis ... | 2014 | 24825333 |
| cost-effectiveness analysis of annual trichomonas vaginalis screening and treatment in hiv-positive women to prevent hiv transmission. | because of a high incidence of trichomonas infection among hiv-positive women, annual screening and treatment are recommended. trichomonas infection is associated with a 2-fold risk of hiv transmission. the objective of this study was to determine if annual screening is cost-effective for the prevention of new hiv cases in susceptible male partners secondary to trichomonas infection in hiv-positive women. | 2014 | 24827616 |
| response letter to v tittle et al. – is test of cure necessary in all cases of trichomonas vaginalis? | 2014 | 24829048 | |
| regulation of nuclear translocation of the myb1 transcription factor by tvcyclophilin 1 in the protozoan parasite trichomonas vaginalis. | in trichomonas vaginalis, a myb1 protein was previously demonstrated to repress transcription of an iron-inducible ap65-1 gene. in this study, a human cyclophilin a homologue, tvcyclophilin 1 (tvcyp1), was identified as a myb1-binding protein using a bacterial two-hybrid library screening system. the recombinant tvcyp1 exhibited typical peptidyl-prolyl isomerase activity with kcat/km of ∼7.1 μm(-1) s(-1). in a pulldown assay, the his-tagged myb1 interacted with a gst-tvcyp1 fusion protein, which ... | 2014 | 24831011 |
| prevalence of bacterial vaginosis in grenadian women of reproductive age. | to determine the prevalence of bacterial vaginosis (bv) among grenadian women of reproductive age using the routinely collected pap smear data for the period january 2009 to december 2011. | 2013 | 24831896 |
| performance of the affirm vp-iii using residual vaginal discharge collected from the speculum to characterize vaginitis in symptomatic women. | to evaluate the ability of collecting the affirm vp-iii test sample using the residual vaginal discharge found on the speculum. | 2014 | 24832170 |
| the tc1/mariner transposable element family shapes genetic variation and gene expression in the protist trichomonas vaginalis. | trichomonas vaginalis is the most prevalent non-viral sexually transmitted parasite. although the protist is presumed to reproduce asexually, 60% of its haploid genome contains transposable elements (tes), known contributors to genome variability. the availability of a draft genome sequence and our collection of >200 global isolates of t. vaginalis facilitate the study and analysis of te population dynamics and their contribution to genomic variability in this protist. | 2014 | 24834134 |
| trichomonas vaginalis homolog of macrophage migration inhibitory factor induces prostate cell growth, invasiveness, and inflammatory responses. | the human-infective parasite trichomonas vaginalis causes the most prevalent nonviral sexually transmitted infection worldwide. infections in men may result in colonization of the prostate and are correlated with increased risk of aggressive prostate cancer. we have found that t. vaginalis secretes a protein, t. vaginalis macrophage migration inhibitory factor (tvmif), that is 47% similar to human macrophage migration inhibitory factor (humif), a proinflammatory cytokine. because humif is report ... | 2014 | 24843155 |
| microscopy outperformed in a comparison of five methods for detecting trichomonas vaginalis in symptomatic women. | in the uk, despite its low sensitivity, wet mount microscopy is often the only method of detecting trichomonas vaginalis infection. a study was conducted in symptomatic women to compare the performance of five methods for detecting t. vaginalis: an in-house polymerase chain reaction (pcr); aptima t. vaginalis kit; osom ®trichomonas rapid test; culture and microscopy. symptomatic women underwent routine testing; microscopy and further swabs were taken for molecular testing, osom and culture. a tr ... | 2015 | 24855131 |
| anti-trichomonas vaginalis activity from triterpenoid derivatives. | trichomonas vaginalis is a flagellated parasite that causes trichomonosis, the most common non-viral sexually transmitted disease (std) in the world. worryingly, trichomonosis is associated to increased transmission of hiv. due to high frequency of the infection during pregnancy and the development of metronidazole-resistant isolates, therapeutic alternatives to 5-nitroimidazole are being searched. triterpenes are natural products presenting several biological activities such as anti-protozoal a ... | 2014 | 24880238 |
| a recently transferred cluster of bacterial genes in trichomonas vaginalis--lateral gene transfer and the fate of acquired genes. | lateral gene transfer (lgt) has recently gained recognition as an important contributor to some eukaryote proteomes, but the mechanisms of acquisition and fixation in eukaryotic genomes are still uncertain. a previously defined norm for lgts in microbial eukaryotes states that the majority are genes involved in metabolism, the lgts are typically localized one by one, surrounded by vertically inherited genes on the chromosome, and phylogenetics shows that a broad collection of bacterial lineages ... | 2014 | 24898731 |
| hptn 035 phase ii/iib randomised safety and effectiveness study of the vaginal microbicides buffergel and 0.5% pro 2000 for the prevention of sexually transmitted infections in women. | to estimate the effectiveness of candidate microbicides buffergel and 0.5% pro 2000 gel (p) (pro 2000) for prevention of non-ulcerative sexually transmitted infections (stis). | 2014 | 24898857 |
| potentiating metronidazole scaffold against resistant trichomonas: design, synthesis, biology and 3d-qsar analysis. | metronidazole (mtz), the fda-approved drug against trichomonas vaginalis (tv), is being challenged seriously by drug resistance, while its inertness to sperm makes it ineffective as a vaginal contraceptive. thirteen piperidine dithiocarbamate hybrids of 2-(2-methyl-5-nitro-1h-imidazol-1-yl)ethane (8-20) were designed to potentiate the mtz framework against drug resistance and sperm. new compounds were 1.2-12.1 times more effective against mtz-susceptible and -resistant strains of tv. all of the ... | 2012 | 24900434 |
| leishmania donovani eukaryotic initiation factor 5a: molecular characterization, localization and homology modelling studies. | eukaryotic translation initiation factor 5a (eif5a) is a small acidic protein highly conserved from archaea to mammals. eif5a is the only protein which undergoes a unique lysine residue modification to hypusine. hypusinylation is important for the function of eif5a which is reported to be essential for cell viability. eif5a promotes formation of the first peptide bond at the onset of protein synthesis. however, its function in leishmania donovani is unclear. the present study focuses on the char ... | 2014 | 24909104 |
| in and out of the minor groove: interaction of an at-rich dna with the drug cd27. | the dna of several pathogens is very rich in at base pairs. typical examples include the malaria parasite plasmodium falciparum and the causative agents of trichomoniasis and trypanosomiases. this fact has prompted studies of drugs which interact with the minor groove of dna, some of which are used in medical practice. previous studies have been performed almost exclusively with the aatt sequence. new features should be uncovered through the study of different dna sequences. in this paper, the c ... | 2014 | 24914972 |
| the influence of art on the treatment of trichomonas vaginalis among hiv-infected women. | among women who are human immunodeficiency virus positive (hiv+), both prevalent and persistent infections with trichomonas vaginalis (tv) are common. tv has been shown to increase vaginal shedding of hiv, which may influence hiv sexual and perinatal transmission, making prevention important. in 1 cohort of hiv+ women in kenya, antiretroviral therapy (art) use, mostly nevirapine based, was associated with lower cure rates of tv for single-dose therapy. our goal was to repeat this study in a us-b ... | 2014 | 24917661 |
| trichomonas stableri n. sp., an agent of trichomonosis in pacific coast band-tailed pigeons (patagioenas fasciata monilis). | trichomonas gallinae is a ubiquitous flagellated protozoan parasite, and the most common etiologic agent of epidemic trichomonosis in columbid and passerine species. in this study, free-ranging pacific coast band-tailed pigeons (patagioenas fasciata monilis) in california (usa) were found to be infected with trichomonad protozoa that were genetically and morphologically distinct from t. gallinae. in microscopic analysis, protozoa were significantly smaller in length and width than t. gallinae an ... | 2014 | 24918075 |
| sensitive simultaneous detection of seven sexually transmitted agents in semen by multiplex-pcr and of hpv by single pcr. | sexually transmitted diseases (stds) may impair sperm parameters and functions thereby promoting male infertility. to date limited molecular studies were conducted to evaluate the frequency and type of such infections in semen thus, we aimed at conceiving and validating a multiplex pcr (m-pcr) assay for the simultaneous detection of the following std pathogens in semen: chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium, trichomonas vaginalis, herpes virus simplex (hsv) -1 and - ... | 2014 | 24921247 |
| prevalence of trichomonas vaginalis, mycoplasma genitalium and ureaplasma urealyticum in men with urethritis attending an urban sexual health clinic. | we conducted a study to determine the prevalence of trichomonas vaginalis (tv), mycoplasma genitalium (mg) and ureaplasma urealyticum (uu) in men with urethritis, attending an urban sexual health clinic, in order to inform screening and treatment policies. men attending an urban sexual health clinic between june 2011 and january 2012 were evaluated. urine samples were collected from men with urethritis and tested for chlamydia trachomatis (ct), neisseria gonorrhoeae (gc) and tv using transcripti ... | 2015 | 24925897 |
| editorial commentary: trichomonas vaginalis testing and screening in a high-risk population: is this a glimpse into the future? | 2014 | 24928287 | |
| added benefit of nucleic acid amplification testing for the diagnosis of trichomonas vaginalis among men and women attending a sexually transmitted diseases clinic. | trichomonas vaginalis (tv) is the most common nonviral sexually transmitted infection (sti) in the world. however, tv is not a reportable sti and, with the exception of hiv-positive women, there are no guidelines for screening in women or men. the objective of this study was to determine the added value of nucleic acid amplification tests (naats) for detection of tv in men and women at high risk for infection as well as correlates of infection. | 2014 | 24928292 |
| antibacterial, antiviral, and antifungal properties of wines and winery byproducts in relation to their flavonoid content. | grapes produce organic compounds that may be involved in the defense of the plants against invading phytopathogens. these metabolites include numerous phenolic compounds that are also active against human pathogens. grapes are used to produce a variety of wines, grape juices, and raisins. grape pomace, seeds, and skins, the remains of the grapes that are a byproduct of winemaking, also contain numerous bioactive compounds that differ from those found in grapes and wines. this overview surveys an ... | 2014 | 24945318 |
| what is the importance of zoonotic trichomonads for human health? | trichomonads are common parasites of many vertebrate and invertebrate species, with four species classically recognized as human parasites: dientamoeba fragilis, pentatrichomonas hominis, trichomonas vaginalis, and trichomonas tenax. the latter two species are considered human-specific; by contrast, d. fragilis and p. hominis have been isolated from domestic and farm mammals, demonstrating a wide host range and potential zoonotic origin. several new studies have highlighted the zoonotic dimensio ... | 2014 | 24951156 |
| modulation of dendritic cell function by trichomonas vaginalis-derived secretory products. | trichomoniasis caused by the parasitic protozoan trichomonas vaginalis is the most common sexually transmitted disease in the world. dendritic cells are antigen presenting cells that initiate immune responses by directing the activation and differentiation of naïve t cells. in this study, we analyzed the effect of trichomonas vaginalis-derived secretory products on the differentiation and function of dendritic cells. differentiation of bone marrow-derived dendritic cells in the presence of t. va ... | 2015 | 24965578 |
| the pathogenic microorganisms in papanicolaou vaginal smears and correlation with inflammation. | non-specific cervicitis or inflammatory changes in a smear report are common which are usually unclear for clinical approaches. to investigate the frequency of inflammation and pathogenic vaginal microorganisms in cervical smears among an iranian population sample. | 2013 | 24971098 |
| expression and characterization of a β-fructofuranosidase from the parasitic protist trichomonas vaginalis. | trichomonas vaginalis, a flagellated protozoan, is the agent responsible for trichomoniasis, the most common nonviral sexually transmitted infection worldwide. a reported 200 million cases are documented each year with far more cases going unreported. however, t. vaginalis is disproportionality under studied, especially considering its basic metabolism. it has been reported that t. vaginalis does not grow on sucrose. nevertheless, the t. vaginalis genome contains some 11 putative sucrose transpo ... | 2014 | 24972630 |
| modified pehps medium as an alternative for the in vitro culture of giardia lamblia. | commercial culture media present interlot variations in biological activity. we have previously designed a homemade and economic culture medium, pehps medium, for the axenic cultivation of entamoeba histolytica and trichomonas vaginalis. trophozoites of amoebae and trichomonads grow well in this medium. furthermore, the medium is stable for several months when stored frozen or refrigerated. the objective of this work was to modify pehps medium to support the in vitro growth of giardia lamblia. i ... | 2014 | 24982905 |
| sexually transmitted infections and prostate cancer risk: a systematic review and meta-analysis. | prostate cancer (pc) is the second most incident cancer and the sixth cause of death by cancer in men worldwide. despite extensive research efforts, no modifiable risk factors have been consistently identified for pc risk. a number of studies have focused on possible relationships between sexually transmitted infections (stis) and pc. we performed a meta-analysis to explore the association between infection caused by neisseria gonorrheae, treponema pallidum, chlamydia trachomatis, trichomonas va ... | 2014 | 24986642 |
| recent advances in molecular biology of parasitic viruses. | the numerous protozoa that can inhabit the human gastro-intestinal tract are known, yet little is understood of the viruses which infect these protozoa. the discovery, morphologic details, purification methods of virus-like particles, genome and proteome of the parasitic viruses, entamoeba histolytica, giardia lamblia, trichomonas vaginalis, and the eimeria sp. are described in this review. the protozoan viruses share many common features: most of them are rna or double-stranded rna viruses, ran ... | 2014 | 25019235 |
| epidemiology of curable sexually transmitted infections among women at increased risk for hiv in northwestern tanzania: inadequacy of syndromic management. | curable, non-viral pathogens account for a significant burden of sexually transmitted infections (stis), and there is established evidence that stis increase both hiv acquisition and transmission. we investigated the prevalence, trends, and factors associated with chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis and treponema pallidum, and the performance of syndromic management, among a cohort of women working in bars, hotels, and other food and recreational facilities near l ... | 2014 | 25025338 |
| design and synthesis of γ-butyrolactone derivatives as potential spermicidal agents. | a series of γ-butyrolactone derivatives has been designed and synthesized from commercially available 2-acetyl butyrolactone (3-acetyldihydrofuran-2(3h)-one, 1) by aminoalkylating its active methylene followed by condensation with different aldehydes. compounds having amino group were further converted to their respective tartrate salts and were evaluated for spermicidal activity against human sperm in vitro. compounds showing appreciable spermicidal activity at ⩽0.5% [3c, 4d (0.5%); 2c, 3d (0.1 ... | 2014 | 25027939 |
| in silico single strand melting curve: a new approach to identify nucleic acid polymorphisms in totiviridae. | the pcr technique and its variations have been increasingly used in the clinical laboratory and recent advances in this field generated new higher resolution techniques based on nucleic acid denaturation dynamics. the principle of these new molecular tools is based on the comparison of melting profiles, after denaturation of a dna double strand. until now, the secondary structure of single-stranded nucleic acids has not been exploited to develop identification systems based on pcr. to test the p ... | 2014 | 25030031 |
| whole slide imaging of pap cell block preparations versus liquid-based thin-layer cervical cytology: a comparative study evaluating the detection of organisms and nonneoplastic findings. | cervical cancer is one of the most common malignancies worldwide, yet it is preventable by population screening. in a previous study, we confirmed the feasibility of utilizing whole slide imaging (wsi) of cell block (cb) preparations to overcome the limitations of digitizing cytologic samples. in this study, we evaluated the accuracy of wsi in identifying various organisms and nonneoplastic findings. | 2014 | 25033897 |
| chemical composition of essential oil and anti trichomonas activity of leaf, stem, and flower of rheum ribes l. extracts. | trichomoniasis is one of the most common sexually transmitted diseases in humans and is caused by the protozoan trichomonas vaginalis. nowadays, increasing resistance to drugs such as metronidazole resulted in many problem, so new effective remedies are needed. in this study, we evaluate constituents of essential oil and anti-trichomonas activity of rheum ribes. | 2014 | 25050317 |
| trichomonas vaginalis: unusual presentation with ulcerative lesions. | 2014 | 25070617 | |
| sexually transmitted infections in women. | sexually transmitted infections (stis) are highly prevalent and cause a wide spectrum of disease. however, the majority of these infections may be unrecognized due to lack of overt signs or symptoms of infection. asymptomatic infections remain significant as a result of the potential for long-term sequelae, predominately in women, and the risks of complications during pregnancy as well as mother-to-child transmission. laboratory diagnostics play an important role in identifying infection and in ... | 2014 | 25083897 |
| clinical characteristics associated with mycoplasma genitalium among female sex workers in nairobi, kenya. | the prevalence of mycoplasma genitalium is high in vulnerable populations of women in low-resource settings. however, the epidemiology of infection in these populations is not well established. to determine the prevalence of mycoplasma genitalium and its association with cervical cytology and other correlates, we recruited 350 female sex workers (fsw) who were 18 to 50 years old in nairobi, kenya, for a cross-sectional study. a questionnaire was administered at baseline to obtain information on ... | 2014 | 25100823 |
| detection of infectious organisms in archival prostate cancer tissues. | seroepidemiological studies have reported associations between exposure to sexually transmitted organisms and prostate cancer risk. this study sought dna evidence of candidate organisms in archival prostate cancer tissues with the aim of assessing if a subset of these cancers show any association with common genital infections. | 2014 | 25106851 |
| a sequential procedure for rapid and accurate identification of putative trichomonacidal agents. | in the current report, a sequential step-wise methodology based on in silico, in vitro and in vivo experimental procedures for the prompt detection of potential trichomonacidal drugs is proposed. a combinatorial of 12 qsar (quantitative structure-activity relationship) models based on linear discrimination analysis (lda) are suggested for the rational identification of new trichomonacidal drugs from virtual screening of in house chemical libraries and drug databases. subsequently, compounds sele ... | 2014 | 25107376 |
| defining genital tract cytokine signatures of sexually transmitted infections and bacterial vaginosis in women at high risk of hiv infection: a cross-sectional study. | sexually transmitted infections (sti) and bacterial vaginosis (bv) cause female genital tract inflammation. this inflammation, which is often present in the absence of symptoms, is associated with increased susceptibility to hiv infection. we aimed to evaluate genital cytokine profiles and the degree of inflammation associated with common stis and bv. | 2014 | 25107710 |
| prevalence of trichomonas vaginalis infection in kashan city, iran (2012-2013). | trichomonas vaginalis infection is one of the most common sexually transmitted diseases in humans. t.vaginalis is a parasitic protozoan with a predilection for human urogenital tract and causative agent for vaginitis, cervicitis and urethritis in females. t.vaginalis infection is associated with risk of human immunodeficiency virus infectivity and pregnancy complication. | 2014 | 25114674 |
| trichomonas vaginalis infections are rare among young patients attending an sti clinic in sweden. | 2015 | 25137630 | |
| phylogenomic study indicates widespread lateral gene transfer in entamoeba and suggests a past intimate relationship with parabasalids. | lateral gene transfer (lgt) has impacted the evolutionary history of eukaryotes, though to a lesser extent than in bacteria and archaea. detecting lgt and distinguishing it from single gene tree artifacts is difficult, particularly when considering very ancient events (i.e., over hundreds of millions of years). here, we use two independent lines of evidence--a taxon-rich phylogenetic approach and an assessment of the patterns of gene presence/absence--to evaluate the extent of lgt in the parasit ... | 2014 | 25146649 |
| the vaginal microbiota: what have we learned after a decade of molecular characterization? | we conducted a systematic review of the medline database (u.s. national library of medicine, national institutes of health, bethesda, md, u.s.a) to determine if consistent molecular vaginal microbiota (vmb) composition patterns can be discerned after a decade of molecular testing, and to evaluate demographic, behavioral and clinical determinants of vmb compositions. studies were eligible when published between 1 january 2008 and 15 november 2013, and if at least one molecular technique (sequenci ... | 2014 | 25148517 |
| unusually low prevalence of mycoplasma genitalium in urine samples from infertile men and healthy controls: a prevalence study. | to detect mycoplasma genitalium in urine samples of infertile men and men without any signs of infection in order to investigate whether m. genitalium and other genital mycoplasmas (mycoplasma hominis and ureaplasma spp) are found more often in urine samples of infertile men than in asymptomatic controls and to determine resistance to macrolides. | 2014 | 25157184 |
| successful treatment of refractory trichomonas vaginalis infection using intravenous metronidazole. | trichomonas vaginalis is a sexually transmitted protozoan infection resulting in a vulvo-vaginitis and altered vaginal discharge in symptomatic women. since its introduction in the 1960 s, metronidazole has been the first-line drug for trichomonal infection. other nitroimidazoles, such as tinidazole, are used as alternative regimens with similar activity but at a greater expense. treatment failure usually represents patient non-compliance or reinfection, although metronidazole resistance has pre ... | 2015 | 25161176 |
| a service evaluation of the gen-probe aptima nucleic acid amplification test for trichomonas vaginalis: should it change whom we screen for infection? | a service evaluation of the new gen-probe aptima nucleic acid amplification test was performed to determine the prevalence of trichomonas vaginalis (tv) infection in a uk sexual health clinic and identify risk factors to inform an appropriate tv screening strategy. | 2014 | 25170162 |
| gonococcal polyarthritis with sternoclavicular joint involvement in pregnant woman: a case report. | pregnancy is one of conditions that increase the risk of gonococcal arthritis which result from blood dissemination of neisseria gonorrhoeae. a 20-year-old africain female patient (in the second trimester of pregnancy), was admitted to hospital because of fever, asymmetric joint swollen affecting the hands, wrists, left ankle, and right sternoclavicular joint. laboratory findings (erythrocyte sedimentation rate was 117 mm in first hour, the serum c-reactive protein level was 152 mg/l) the gram s ... | 2014 | 25170386 |
| comparative evaluation of roche aurora flow, becton and dickinson viper system, and dynex ds2 for detection of chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis in various clinical specimens. | the purpose of this study is to introduce a high-throughput system, aurora flow, for the simultaneous detection of 3 clinically relevant pathogens of sexually transmitted infections. comparative evaluation with other systems revealed an overall concordance of 97.9% for chlamydia trachomatis and comparable performance for neisseria gonorrhoeae and trichomonas vaginalis. | 2014 | 25172824 |
| trichomonas vaginalis infection among young pregnant women in brazil. | our goal was to determine the prevalence of trichomonas vaginalis and its associated risk factors in parturient women aged 15-24 years attending brazilian public maternity units. participants answered a demographic, behavioral, and clinical data questionnaire. a sample of urine was screened for t. vaginalis. a total 299 women participated in this study. the prevalence rate of t. vaginalis was 7.7% (95% ci: 4.7-10.7%). the factors associated with t. vaginalis were use of illicit drugs [or=4.70 (9 ... | 2014 | 25181400 |
| signal transduction triggered by iron to induce the nuclear importation of a myb3 transcription factor in the parasitic protozoan trichomonas vaginalis. | iron was previously shown to induce rapid nuclear translocation of a myb3 transcription factor in the protozoan parasite, trichomonas vaginalis. in the present study, iron was found to induce a transient increase in cellular camp, followed by the nuclear influx of myb3, whereas the latter was also induced by 8-bromo-cyclic amp. iron-inducible camp production and nuclear influx of myb3 were inhibited by suramin and sq22536, respective inhibitors of the gα subunit of heterotrimeric g proteins and ... | 2014 | 25183012 |
| comparative protein structure modeling using modeller. | functional characterization of a protein sequence is one of the most frequent problems in biology. this task is usually facilitated by accurate three-dimensional (3-d) structure of the studied protein. in the absence of an experimentally determined structure, comparative or homology modeling can sometimes provide a useful 3-d model for a protein that is related to at least one known protein structure. comparative modeling predicts the 3-d structure of a given protein sequence (target) based prim ... | 2014 | 25199792 |
| trichocystatin-2 (tc-2): an endogenous inhibitor of cysteine proteinases in trichomonas vaginalis is associated with tvcp39. | the causal agent of trichomoniasis is a parasitic protist, trichomonas vaginalis, which is rich in proteolytic activity, primarily carried out by cysteine proteases (cps). some cps are known virulence factors. t. vaginalis also possesses three genes encoding endogenous cystatin-like cp inhibitors. the aim of this study was to identify and characterize one of these cp inhibitors. using two-dimensional gel electrophoresis (2-de) and mass spectrometry (ms), a cystatin-like peptidase inhibitor dubbe ... | 2014 | 25200185 |
| epidemiology of sexually transmitted infections in rural southwestern haiti: the grand'anse women's health study. | the study attempts to define socioeconomic, clinical, and laboratory correlates in vaginitis and other sexually transmitted infections in rural southwestern haiti. a convenience sample of subjects recruited from a rural women's health clinic and attending an established clinic at the haitian health foundation (hhf) clinic was studied. a standardized history and physical examination, including speculum examination, and collection of blood, urine, and vaginal swabs were obtained from the women at ... | 2014 | 25200263 |
| immunity in urogenital protozoa. | innate and adaptive immunity play a significant role in urogenital infections. innate immunity is provided by the epithelial cells and mucus lining along with acidic ph, which forms a strong physical barrier against the pathogens in female reproductive tract. cells of innate immune system, antimicrobial peptides, cytokines, chemokines and adaptive immunity in the reproductive tract are evolved during infection, and a pro-inflammatory response is generated to fight against the invading pathogen t ... | 2014 | 25201404 |
| impact of public housing relocations: are changes in neighborhood conditions related to stis among relocaters? | cross-sectional and ecologic studies suggest that place characteristics influence sexual behaviors and sexually transmitted infections (stis). using data from a predominately substance-misusing cohort of african american adults relocating from us public housing complexes, this multilevel longitudinal study tested the hypothesis that participants who experienced greater postrelocation improvements in neighborhood conditions (i.e., socioeconomic disadvantage, social disorder, sti prevalence, and m ... | 2014 | 25211249 |
| epidemiology of sexually transmitted infections in rural haitian men. | the study attempts to determine the prevalence of organisms associated with urethritis in men in rural southwestern haiti and to determine the association with demographic, clinical and laboratory variables. a standardised verbal interview was conducted; genital examinations were done; urethral swabs were collected for nucleic acid amplification testing, and first void urine was obtained for urinalysis. the mean participant age was 54; 88.8% lived in a rural area. swabs were positive for trichom ... | 2015 | 25228665 |
| high prevalence and incidence of sexually transmitted infections among women living in kwazulu-natal, south africa. | sexually transmitted infections (stis) contribute largely to the burden of health in south africa and are recognized as major contributors to the human immunodeficiency virus (hiv) epidemic. young women are particularly vulnerable to stis. the purpose of this secondary analysis was to examine the risk factors associated with prevalent and incident stis among women who had participated in three clinical trials. | 2014 | 25243015 |
| cultivation of parasites. | parasite cultivation techniques constitute a substantial segment of present-day study of parasites, especially of protozoa. success in establishing in vitro and in vivo culture of parasites not only allows their physiology, behavior and metabolism to be studied dynamically, but also allows the nature of the antigenic molecules in the excretory and secretory products to be vigorously pursued and analyzed. the complex life-cycles of various parasites having different stages and host species requir ... | 0 | 25250227 |
| putrescine-dependent re-localization of tvcp39, a cysteine proteinase involved in trichomonas vaginalis cytotoxicity. | polyamines are involved in the regulation of some trichomonas vaginalis virulence factors such as the transcript, proteolytic activity, and cytotoxicity of tvcp65, a cysteine proteinase (cp) involved in the trichomonal cytotoxicity. in this work, we reported the putrescine effect on tvcp39, other cp that also participate in the trichomonal cytotoxicity. parasites treated with 1,4-diamino-2-butanone (dab) (an inhibitor of putrescine biosynthesis), diminished the amount and proteolytic activity of ... | 2014 | 25251406 |
| prevalence of trichomonas vaginalis, chlamydia trachomatis, neisseria gonorrhoeae and human papillomavirus in a sexual health clinic setting in urban sri lanka. | the prevalences of trichomonas vaginalis, chlamydia trachomatis, neisseria gonorrhoeae, and human papillomavirus (hpv) in sri lanka are not well reported; the objective of this study is to describe the prevalences of these four sexually transmitted infections among attendees of sexual health clinic in an urban setting. vaginal swabs were collected from consenting women attending a sexual health clinic and tested for the presence of the above sexually transmitted infections using nucleic acid amp ... | 2015 | 25258396 |
| genetic characterization of oropharyngeal trichomonad isolates from wild birds indicates that genotype is associated with host species, diet and presence of pathognomonic lesions. | oropharyngeal trichomonad isolates of wild birds from spain were studied. a total of 1688 samples (1214 of predator birds and 474 of prey species) from wildlife recovery centres and scientific bird-ringing campaigns were analysed from 2011 to 2013. the overall infection prevalence was 20.3% (11.4% in predator birds and 43.3% in prey species). pathognomonic lesions were present in 26% of the infected birds (57.3% in predator birds and 4.9% in prey species). the most commonly parasitized species w ... | 2014 | 25262786 |
| trichomonas gypaetinii n. sp., a new trichomonad from the upper gastrointestinal tract of scavenging birds of prey. | in the context of an epidemiological study carried out by several wildlife recovery centers in spain, trichomonads resembling trichomonas gallinae were found in the oropharyngeal cavity of 2 egyptian vultures (neophron percnopterus) and 14 cinereous vultures (aegypius monachus) which did not show any symptoms of trichomonosis. in order to characterize them, these isolates along with seven other t. gallinae isolates obtained from different hosts and from different geographical origin were analyze ... | 2015 | 25273632 |
| identification of cytolytic vaginosis versus vulvovaginal candidiasis. | this study aimed to observe the morphological characteristic of vaginal discharge in patients with cytolytic vaginosis (cv) under the microscope and to identify it in patients with cv and in patients with vulvovaginal candidiasis (vvc). | 2015 | 25279977 |
| trichomonas vaginalis induces cytopathic effect on human lung alveolar basal carcinoma epithelial cell line a549. | trichomonas vaginalis, the causative agent of trichomoniasis is generally known to inhabit the genitourinary tract. however, several case reports with supporting molecular and immunological identifications have documented its occurrence in the respiratory tract of neonates and adults. in addition, the reports have documented that its occurrence is associated with respiratory failures. the medical significance or consequence of this association is unclear. thus, to establish the possible outcome ... | 2014 | 25307688 |
| prevalence and risk factors for chlamydia trachomatis, neisseria gonorrhoeae and trichomonas vaginalis infection in pregnant women in papua new guinea. | to determine the prevalence of, and risk factors associated with, chlamydia trachomatis, neisseria gonorrhoeae and trichomonas vaginalis infection in pregnant women in madang, papua new guinea (png). | 2015 | 25313204 |
| drug-free chitosan coated poly(isobutylcyanoacrylate) nanoparticles are active against trichomonas vaginalis and non-toxic towards pig vaginal mucosa. | the present work reports a non-conventional therapeutic strategy based on the use of vaginally-applied formulations for the treatment of trichomoniasis due to trichomonas vaginalis without adding a drug. | 2015 | 25319099 |
| the parasite trichomonas vaginalis expresses thousands of pseudogenes and long non-coding rnas independently from functional neighbouring genes. | the human pathogen trichomonas vaginalis is a parabasalian flagellate that is estimated to infect 3% of the world's population annually. with a 160 megabase genome and up to 60,000 genes residing in six chromosomes, the parasite has the largest genome among sequenced protists. although it is thought that the genome size and unusual large coding capacity is owed to genome duplication events, the exact reason and its consequences are less well studied. | 2014 | 25326207 |
| male infertility: a public health issue caused by sexually transmitted pathogens. | sexually transmitted diseases (stds) are caused by several pathogens, including bacteria, viruses and protozoa, and can induce male infertility through multiple pathophysiological mechanisms. additionally, horizontal transmission of std pathogens to sexual partners or vertical transmission to fetuses and neonates is possible. chlamydia trachomatis, ureaplasma spp., human papillomavirus, hepatitis b and hepatitis c viruses, hiv-1 and human cytomegalovirus have all been detected in semen from symp ... | 2014 | 25330794 |
| an emerging mycoplasma associated with trichomoniasis, vaginal infection and disease. | humans are colonized by thousands of bacterial species, but it is difficult to assess the metabolic and pathogenic potential of the majority of these because they have yet to be cultured. here, we characterize an uncultivated vaginal mycoplasma tightly associated with trichomoniasis that was previously known by its 16s rrna sequence as "mnola." in this study, the mycoplasma was found almost exclusively in women infected with the sexually transmitted pathogen trichomonas vaginalis, but rarely obs ... | 2014 | 25337710 |
| biological roles of cysteine proteinases in the pathogenesis of trichomonas vaginalis. | human trichomonosis, infection with trichomonas vaginalis, is the most common non-viral sexually transmitted disease in the world. the host-parasite interaction and pathophysiological processes of trichomonosis remain incompletely understood. this review focuses on the advancements reached in the area of the pathogenesis of t. vaginalis, especially in the role of the cysteine proteinases. it highlights various approaches made in this field and lists a group of trichomonad cysteine proteinases in ... | 2014 | 25348828 |
| multisite direct determination of the potential for environmental contamination of urine samples used for diagnosis of sexually transmitted infections. | the detection of a sexually transmitted infection (sti) agent in a urine specimen from a young child is regarded as an indicator of sexual contact. false positives may conceivably arise from the transfer of environmental contaminants in clinic toilet or bathroom facilities into urine specimens. | 2013 | 25349693 |
| coinfection with chlamydia trachomatis, neisseria gonorrhoeae and trichomonas vaginalis: a cross-sectional analysis of positivity and risk factors in remote australian aboriginal communities. | to determine the co-occurrence and epidemiological relationships of chlamydia trachomatis (ct), neisseria gonorrhoeae (ng) and trichomonas vaginalis (tv) in a high-prevalence setting in australia. | 2015 | 25352691 |
| prevalence of trichomonas vaginalis by pcr in men attending a primary care urology clinic in south korea. | trichomonas vaginalis, a causative agent of trichomoniasis, may trigger symptomatic or asymptomatic nongonococcal urethritis and chronic prostatitis in men. despite the availability of highly sensitive diagnostic tests, such as nucleic acid amplification tests, including pcr, few prospective studies present data on male t. vaginalis infection in south korea. in the present study, the prevalence of t. vaginalis and associated clinical conditions were evaluated in 201 male patients from a primary ... | 2014 | 25352707 |
| incidence of curable sexually transmissible infections among adolescents and young adults in remote australian aboriginal communities: analysis of longitudinal clinical service data. | to undertake the first comprehensive analysis of the incidence of three curable sexually transmissible infections (stis) within remote australian aboriginal populations and provide a basis for developing new control initiatives. | 2015 | 25371420 |
| three-dimensional structure of a protozoal double-stranded rna virus that infects the enteric pathogen giardia lamblia. | giardia lamblia virus (glv) is a small, nonenveloped, nonsegmented double-stranded rna (dsrna) virus infecting giardia lamblia, the most common protozoan pathogen of the human intestine and a major agent of waterborne diarrheal disease worldwide. glv (genus giardiavirus) is a member of family totiviridae, along with several other groups of protozoal or fungal viruses, including leishmania rna viruses and trichomonas vaginalis viruses. interestingly, glv is more closely related than other totivir ... | 2014 | 25378500 |
| trichomonas vaginalis: clinical relevance, pathogenicity and diagnosis. | trichomonas vaginalis is the etiological agent of trichomoniasis, the most prevalent non-viral sexually transmitted disease worldwide. trichomoniasis is a widespread, global health concern and occurring at an increasing rate. infections of the female genital tract can cause a range of symptoms, including vaginitis and cervicitis, while infections in males are generally asymptomatic. the relatively mild symptoms, and lack of evidence for any serious sequelae, have historically led to this disease ... | 2016 | 25383648 |
| abuse impedes prevention: the intersection of intimate partner violence and hiv/sti risk among young african american women. | intimate partner violence (ipv) is associated with risky sexual behavior and stis among diverse groups of women. ipv was examined as a moderator of efficacy for an hiv/sti intervention. 848 african american women, 18-29, were randomly assigned to an hiv/sti intervention or control condition. participants completed measures on sociodemographics, ipv, risky sexual behavior and received sti testing. ipv predicted inconsistent condom use and a risky sexual partner over 12-month follow-up. a signific ... | 2015 | 25399033 |
| prevalence and risk factors for cervical neoplasia: a cervical cancer screening program in beijing. | cervical cancer is the second most common cancer and cause of cancer-related death for women worldwide. the aims of this study were to investigate the prevalence of cervical neoplasia and examine factors associated with high-grade cervical squamous intraepithelial lesions (hsil) among women taking part in a cervical cancer screening program in beijing. | 2014 | 25410572 |
| deoxyadenosine family: improved synthesis, dna damage and repair, analogs as drugs. | improved synthesis of 2'-deoxyadenosine using escherichia coli overexpressing some enzymes and gram-scale chemical synthesis of 2'-deoxynucleoside 5'-triphosphates reported recently are described in this review. other topics include dna damage induced by chromium(vi), fenton chemistry, photoinduction with lumazine, or by ultrasound in neutral solution; 8,5'-cyclo-2'-deoxyadenosine isomers as potential biomarkers; and a recapitulation of purine 5',8-cyclonucleoside studies. the mutagenicities of ... | 2013 | 25436589 |
| gene-expression analysis of cold-stress response in the sexually transmitted protist trichomonas vaginalis. | trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common nonviral sexually transmitted disease in the world. this infection affects millions of individuals worldwide annually. although direct sexual contact is the most common mode of transmission, increasing evidence indicates that t. vaginalis can survive in the external environment and can be transmitted by contaminated utensils. we found that the growth of t. vaginalis under cold conditions is greatly inhibited, but rec ... | 2015 | 25440978 |
| design and synthesis of substituted morpholin/piperidin-1-yl-carbamodithioates as promising vaginal microbicides with spermicidal potential. | a series of seventeen morpholin/piperidin-1-yl-carbamodithioate (3-19) were synthesized as topical vaginal microbicidal spermicides. the synthesized compounds were evaluated for their anti-trichomonas activity against mtz susceptible and resistant strains along with their spermicidal and antifungal potential. all the synthesized compounds were assessed for their safety through cytotoxic assay against human cervical cell line (hela) and compatibility with vaginal flora, lactobacillus. the study i ... | 2014 | 25453819 |
| evidence for repeated gene duplications in tritrichomonas foetus supported by est analysis and comparison with the trichomonas vaginalis genome. | tritrichomonas foetus causes a venereal infection in cattle; the disease has mild or no clinical manifestation in bulls, while cows may present vaginitis, placentitis, pyometra and abortion in the more severe cases. t. foetus has one of the largest known genomes among trichomonads. however molecular data are fragmentary and have minimally contributed to the understanding of the biology and pathogenesis of this protozoan. in a search of new t. foetus genes, a detailed exploration was performed us ... | 2014 | 25458117 |
| trichomonas vaginalis origins, molecular pathobiology and clinical considerations. | to integrate a selection of the most recent data on trichomonas vaginalis origins, molecular cell biology and t. vaginalis interactions with the urogenital tract microbiota with trichomoniasis symptoms and clinical management. | 2015 | 25485651 |
| trichomonas vaginalis among women raped in antananarivo, 2005-2011. | rape is a forensic emergency due to the possibility of sexually transmitted infections, including hiv/aids. moreover, an upsurge is currently underway. the objective of this study was to determine the incidence of trichomonas vaginalis in women undergoing tests at the microbiology unit of the joseph ravoahangy andrianavalona antananarivo hospital after being raped. this retrospective descriptive study covers the 7-year period from 2005 to 2011 and reports the results of direct examination of the ... | 2014 | 25499225 |
| the recombinant prepro region of tvcp4 is an inhibitor of cathepsin l-like cysteine proteinases of trichomonas vaginalis that inhibits trichomonal haemolysis. | trichomonas vaginalis expresses multiple proteinases, mainly of the cysteine type (cps). a cathepsin l-like 34kda cp, designated tvcp4, is synthesized as a 305-amino-acid precursor protein. tvcp4 contains the prepro fragment and the catalytic triad that is typical of the papain-like cp family of clan ca. the aim of this work was to determine the function of the recombinant tvcp4 prepro region (pptvcp4r) as a specific inhibitor of cps. we cloned, expressed, and purified the recombinant tvcp4 prep ... | 2015 | 25499446 |
| high prevalence and incidence of asymptomatic sexually transmitted infections during pregnancy and postdelivery in kwazulu natal, south africa. | we report the prevalence and incidence of 3 treatable sexually transmitted pathogens (neiserria gonorrhoeae, chlamydia trachomatis, and trichomonas vaginalis) in women who were hiv infected or at high risk for hiv infection, in pregnancy and postpartum, respectively. | 2015 | 25504300 |
| randomized trial of periodic presumptive treatment with high-dose intravaginal metronidazole and miconazole to prevent vaginal infections in hiv-negative women. | vaginal infections are common, frequently recur, and may increase women's risk for sexually transmitted infections (stis). we tested the efficacy of a novel regimen to prevent recurrent vaginal infections. | 2014 | 25526757 |
| sexually transmitted diseases: epidemiological and clinical aspects in adults. | sexually transmitted diseases (stds) are the first 10 causes of unpleased diseases in young adult women in the world. the concept of stds includes a series of syndromes caused by pathogens that can be acquired by sexual intercourse or sexual activity.adolescents and young adults are responsible for only 25% of the sexually active population and they represent almost 50% of all newly acquired stds.in this way, we evaluated the epidemiological and clinical aspects of most relevant pathogens as nei ... | 2016 | 25532465 |
| performance evaluation of a dna chip assay in the identification of major genitourinary pathogens. | to prevent the recurrence of genitourinary infections and to reduce the risks of their complications, accurate and rapid diagnosis are required. stdetect® chip is a dna chip which allows for the simultaneous detection of 13 major genitourinary pathogens in a single vaginal swab or urine specimen. we evaluated the analytical performance of the stdetect® chip for detecting target pathogens that commonly cause genitourinary infections. | 2015 | 25533217 |
| trichomonas vaginalis metalloproteinase induces mtor cleavage of siha cells. | trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. the mammalian target of rapamycin (mtor) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. we detected various types of metalloproteinases including gp63 protein from t. vaginalis trophozoites, and t. vaginalis gp63 metalloproteinase was confirmed by sequencing and weste ... | 2014 | 25548410 |