Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| bacillus cereus infections in traumatology-orthopaedics department: retrospective investigation and improvement of healthcare practices. | to investigate 41 open fractures infected with bacillus cereus in a traumatology-orthopaedy ward and propose a care protocol at admission. | 2005 | 15603836 |
| high-level expression of recombinant phospholipase c from bacillus cereus in pichia pastoris and its characterization. | the phospholipase c (plc) gene from bacillus cereus was cloned into the ppiczc vector and integrated into the genome of pichia pastoris. the phospholipase c (plc) when expressed in p. pastoris was fused to the alpha-factor secretion signal peptide of saccharomyces cerevisiae and secreted into a culture medium. recombinant p. pastoris x-33 had a clear plc band at 28.5 kda and produced an extracellular plc with an activity of 678 u mg(-1) protein which was more than a recombinant p. pastoris gs115 ... | 2004 | 15604782 |
| enzymatic properties of the membrane-bound nadh oxidase system in the aerobic respiratory chain of bacillus cereus. | membranes prepared from bacillus cereus kctc 3674, grown aerobically on a complex medium, oxidized nadh exclusively, whereas deamino-nadh was little oxidized. the respiratory chain-linked nadh oxidase exhibited an apparent k(m) value of approximately 65 microm for nadh. the maximum activity of the nadh oxidase was obtained at about ph 8.5 in the presence of 0.1 m kcl (or nacl). respiratory chain inhibitor 2-heptyl-4-hydroxyquinoline-n-oxide (hqno) inhibited the activity of the nadh oxidase by ab ... | 2004 | 15607037 |
| essential oils of satureja, origanum, and thymus species: chemical composition and antibacterial activities against foodborne pathogens. | the chemical composition of the essential oils obtained from the species restricted to greece and the eastern mediterranean region, satureja spinosa l. and thymus longicaulis l.; species endemic to central and south greece, satureja parnassica ssp. parnassica heldr. and sart ex boiss.; species endemic to the island of crete, origanum dictamnus l.; and species widely distributed in the mediterranean region, satureja thymbra l. and origanum vulgare l. subsp. hirtum, were determined by gas chromato ... | 2004 | 15612827 |
| antibacterial activity and specificity of the six human {alpha}-defensins. | we developed a kinetic, 96-well turbidimetric procedure that is capable of testing the antimicrobial properties of six human alpha-defensins concurrently on a single microplate. the defensins were prepared by solid-phase peptide synthesis and tested against gram-positive bacteria (staphylococcus aureus and bacillus cereus) and gram-negative bacteria (enterobacter aerogenes and escherichia coli). analysis of the growth curves provided virtual lethal doses (vlds) equivalent to conventional 50% let ... | 2005 | 15616305 |
| phenotypic and genotypic characterisation of bacillus cereus isolates from bangladeshi rice. | we report the first phenotypic and genotypic characterisation of bacillus cereus from bangladeshi rice. seven strains of b. cereus were isolated from two high-yielding rice varieties (br5 and brri dhan28) which are cultivated during different rice-growing seasons in bangladesh. the strains were identified as b. cereus based on colonial and cellular (light microscopic) morphology and were confirmed using the api 50 chb test system and 16s rdna analysis. they could also be distinguished from each ... | 2005 | 15617798 |
| discrimination of pathogenic clinical isolates and laboratory strains of bacillus cereus by nmr-based metabolomic profiling. | six different bacillus cereus strains were selected from two different ecotypes: (1) three commonly used laboratory strains that are considered avirulent, and (2) three clinical isolates from meningitis patients. screening of genomic dna for the presence of genes encoding known toxins gave no candidate genes that were unambiguously able to distinguish between the two groups. however, the application of multivariate pattern-recognition methods to metabolite profiles derived from the different str ... | 2005 | 15621429 |
| heat-stable toxin production by strains of bacillus cereus, bacillus firmus, bacillus megaterium, bacillus simplex and bacillus licheniformis. | strains of bacillus cereus can produce a heat-stable toxin (cereulide). in this study, 101 bacillus strains representing 7 bacillus species were tested for production of heat-stable toxins. strains of b. megaterium, b. firmus and b. simplex were found to produce novel heat-stable toxins, which showed varying levels of toxicity. b. cereus strains (18 out of 54) were positive for toxin production. thirteen were of serovar h1, and it was of interest that some were of clinical origin. two were of se ... | 2005 | 15621453 |
| high-level expression and characterization of two chitinases, chich and chicw, of bacillus cereus 28-9 in escherichia coli. | many chitinase genes have been cloned and sequenced from prokaryotes and eukaryotes but overexpression of chitinases in escherichia coli cells was less reported. chich and chicw of bacillus cereus 28-9 belong to two distinct groups based on their amino acid sequences of catalytic domains, and in addition, domain structures of two enzymes are different. in this study, we established an ideal method for high-level expression of chitinases in e. coli as glutathione-s-transferase fusion proteins usi ... | 2005 | 15629422 |
| emetic toxin formation of bacillus cereus is restricted to a single evolutionary lineage of closely related strains. | an in-depth polyphasic approach was applied to study the population structure of the human pathogen bacillus cereus. to assess the intraspecific biodiversity of this species, which is the causative agent of gastrointestinal diseases, a total of 90 isolates from diverse geographical origin were studied by genetic [m13-pcr, random amplification of polymorphic dna (rapd), multilocus sequence typing (mlst)] and phenetic [fourier transform infrared (ftir), protein profiling, biochemical assays] metho ... | 2005 | 15632437 |
| characterization and antimicrobial activity of bacteriocin 217 produced by natural isolate lactobacillus paracasei subsp. paracasei bgbuk2-16. | the strain lactobacillus paracasei subsp. paracasei bgbuk2-16. which was isolated from traditionally homemade white-pickled cheese, produces bacteriocin 217 (bac217; approximately 7 kda). the onset of bac217 biosynthesis was observed in the logarithmic phase of growth, and the production plateau was reached after 9 or 12 h of incubation at 37 and 30 degrees c, respectively, when culture entered the early stationary phase. biochemical characterization showed that bac217 retained antimicrobial act ... | 2004 | 15633678 |
| incidence and characterization of bacillus cereus isolated from traditional fermented meals in nigeria. | the aim of this study was to examine the presence of bacillus cereus in fermented meals used in food seasoning in nigeria. the microbial profiles of iru and ogiri, two nigerian fermented vegetable proteins, were examined for presence of b. cereus. in the 50 samples tested, b. cereus was detected in all the samples, with the level of detection ranging from log 6.3 to log 8.3 g(-1) sample. phenotypic characteristics of the b. cereus isolates showed that all of them could not ferment many sugars, m ... | 2004 | 15633691 |
| synthesis, surface active and antimicrobial properties of new alkyl 2,6-dideoxy-l-arabino-hexopyranosides. | synthesis of alkyl 2,6-dideoxy-l-arabino-hexopyranosides was accomplished by the reaction of 1,5-anhydro-2,6-dideoxy-l-arabino-hex-1-enitol with fatty alcohols in dichloromethane, catalyzed by triphenylphosphine hydrobromide. reaction with octanol and dodecanol gave the corresponding alpha-glycosides in 50% and 42% yield, the beta-glycosides in 20% and 21% yield and the alpha-anomer of the ferrier product in 10% and 9% yield, respectively. deacetylation of the alpha-/beta-glycosides with sodium ... | 2005 | 15639239 |
| identification and partial characterization of the nonribosomal peptide synthetase gene responsible for cereulide production in emetic bacillus cereus. | cereulide, a depsipeptide structurally related to valinomycin, is responsible for the emetic type of gastrointestinal disease caused by bacillus cereus. due to its chemical structure, (d-o-leu-d-ala-l-o-val-l-val)(3), cereulide might be synthesized nonribosomally. therefore, degenerate pcr primers targeted to conserved sequence motifs of known nonribosomal peptide synthetase (nrps) genes were used to amplify gene fragments from a cereulide-producing b. cereus strain. sequence analysis of one of ... | 2005 | 15640177 |
| inactivation of bacterial endospores by photocatalytic nanocomposites. | a novel biocidal photocatalytic nanocomposite, composed of tio(2) and multi-walled carbon nanotubes (mwnts), was synthesized via wet chemistry followed by a heat treatment. uniform anatase coatings on mwnts were successfully obtained with a thickness of a few nanometers. the nanostructure of the composite was determined by high resolution transmission electron microscopy (hrtem) and x-ray diffraction (xrd). the needle-like shape of the nanocomposite provided more than three times higher photocat ... | 2005 | 15642459 |
| penetration pharmacokinetics of topically administered 0.5% moxifloxacin ophthalmic solution in human aqueous and vitreous. | to investigate the penetration of 0.5% moxifloxacin hydrochloride into the aqueous and vitreous after topical administration in humans. | 2005 | 15642810 |
| synthesis and antibacterial activity of 4-benzoyl-1-methyl-5-phenyl-1h-pyrazole-3-carboxylic acid and derivatives. | some new 1h-pyrazole-3-carboxylic acid and pyridazinone derivatives were synthesized and evaluated for their antibacterial activities against bacillus cereus atcc 7064, staphylococcus aureus atcc 6538, escherichia coli atcc 4230 and pseudomonas putida using tube dilution method. the minimal inhibitory concentrations (mics) experiments revealed that all chemical compounds showed inhibitor effects on the growth of the test microorganisms. moreover, the results of this research showed that the comp ... | 2004 | 15652364 |
| fulminant septicemia caused by bacillus cereus following reduced-intensity umbilical cord blood transplantation. | 2005 | 15653460 | |
| specificity and polymorphism of the plcr-papr quorum-sensing system in the bacillus cereus group. | the expression of extracellular virulence factors in various species of the bacillus cereus group is controlled by the plcr and papr genes, which encode a transcriptional regulator and a cell-cell signaling peptide, respectively. a processed form of papr, presumably a pentapeptide, specifically interacts with plcr to facilitate its binding to its dna targets. this activating mechanism is strain specific, with this specificity being determined by the first residue of the pentapeptide. we carried ... | 2005 | 15659693 |
| quantitative analysis of cereulide, an emetic toxin of bacillus cereus, by using rat liver mitochondria. | an emetic toxin cereulide, produced by bacillus cereus, causes emetic food poisonings, but a method for quantitative measurement of cereulide has not been well established. a current detection method is a bioassay method using the hep-2 cell vacuolation test, but it was unable to measure an accurate concentration. we established a quantitative assay for cereulide based on its mitochondrial respiratory uncoupling activity. the oxygen consumption in a reaction medium containing rat liver mitochond ... | 2005 | 15665450 |
| modeling the growth kinetics of bacillus cereus as a function of temperature, ph, sodium lactate and sodium chloride concentrations. | mathematical models describing the growth kinetic parameters (lag phase duration and growth rate) of bacillus cereus as a function of temperature, ph, sodium lactate and sodium chloride concentrations were obtained in this study. in order to get a residual distribution closer to a normal distribution, the natural logarithm of the growth kinetic parameters were used in modeling. for reasons of parsimony, the polynomial models were reduced to contain only the coefficients significant at a level of ... | 2005 | 15681041 |
| production of antimicrobial metabolites by strains of lactobacillus or lactococcus co-cultured with bacillus cereus in milk. | during co-culture of lactobacillus (five strains) or lactococcus (two strains) with bacillus cereus, organic acids and other potentially antimicrobial metabolites are produced. lactic acid was produced at very different rates by the lactic acid bacteria (lab) and the final concentrations varied much, however, the crucial point of rapid ph reduction during the initial hours of fermentation coincides with lactic acid production. moderate amounts of acetic acid were produced during fermentation and ... | 2005 | 15681046 |
| rapid genotypic detection of bacillus anthracis and the bacillus cereus group by multiplex real-time pcr melting curve analysis. | bacillus anthracis has four plasmid possible virulence genotypes: pxo1+/pxo2+, pxo1+/pxo2-, pxo1-/pxo2+ or pxo1-/pxo2-. due to the lack of a specific chromosomal marker for b. anthracis, differentiation of the pxo1-/pxo2- form of b. anthracis from closely related bacillus cereus group species is difficult. in this study, we evaluate the ability of sspe, pxo1 and pxo2 primers to discriminate individual b. anthracis and the b. cereus group genotypes using multiplex real-time pcr and melting curve ... | 2005 | 15681162 |
| pollution status of swimming pools in south-south zone of south-eastern nigeria using microbiological and physicochemical indices. | microbiological and physicochemical characteristics of swimming pools in south eastern states of nigeria (akwa ibom and cross river) were investigated. the bacterial isolates included enterococcus faecalis, clostridium perfringens, bacillus cereus and escherichia coli. others were pseudomonas aeruginosa, staphylococcus aureus, proteus vulgaris and staphylococcus epidermidis, while fungal isolates were penicillium sp, rhizopus sp, aspergillus versicolor fusarium sp, trichophyton mentagrophytes, m ... | 2004 | 15691161 |
| gerr, a novel ger operon involved in l-alanine- and inosine-initiated germination of bacillus cereus atcc 14579. | bacillus cereus endospores germinate in response to particular nutrients. spores are able to sense these nutrients in the environment by receptors encoded by the gera family of operons. analysis of the bacillus cereus atcc 14579 genome revealed seven gera family homologues. using a transposon tn917-based insertional mutagenesis approach followed by an enrichment procedure to select for l-alanine-induced germination mutants, we isolated a mutant with a defect in the l-alanine germination pathway. ... | 2005 | 15691930 |
| behavior of escherichia coli cells and bacillus cereus spores on poplar wood crates by impedance measurements. | dry poplar wood crates and glass surfaces (bottoms of pyrex flasks) were contaminated with aqueous suspensions of escherichia coli collection de l'institut pasteur (cip) 54.8 cells or bacillus cereus cip 78.3 spores. after different periods of storage at 25 degrees c, the number of cells still present on both surfaces was determined by impedance microbiology. the physical and chemical properties of the wood greatly and rapidly decreased the number of cells. after 4 h of contact time, b. cereus r ... | 2005 | 15693168 |
| synthesis of schiff bases of 4-(4-aminophenyl)-morpholine as potential antimicrobial agents. | in the present study, a novel series of schiff bases of 4-(4-aminophenyl)-morpholine were synthesised and characterised by ir, 1h-nmr, 13c-nmr, mass spectral and elemental analysis. the compounds were screened for antibacterial (staphylococcus aureus (atcc 9144), staphylococcus epidermidis (atcc 155), bacillus cereus (atcc 11778), micrococcus luteus (atcc 4678), and escherichia coli (atcc 25922)) and antifungal (candida albicans (atcc 2091) and aspergillus niger (atcc 9029)) activities. the mini ... | 2005 | 15694658 |
| polyphasic approach for identifying bacillus spp. | 2005 | 15695735 | |
| bacillus cereus fur regulates iron metabolism and is required for full virulence. | a homologue of the bacillus subtilis fur gene was identified in bacillus cereus and characterized. the predicted amino acid sequence of the cloned gene was found to be highly similar to other members of the fur family of transcriptional regulators. the b. cereus fur gene was shown to partially complement an escherichia coli fur mutant. purified b. cereus fur bound specifically to a 19 bp dna sequence homologous to the b. subtilis fur box in a metal-dependent manner. analysis of the available b. ... | 2005 | 15699205 |
| fatal bacillus cereus endocarditis masquerading as an anthrax-like infection in a patient with acute lymphoblastic leukemia: case report. | a 38-year-old male farm worker with relapsing acute lymphoblastic leukemia spontaneously developed an ulcerating ulcer on his anterior thigh which was surrounded by a non-tender area of erythema. bacillus cereus was isolated from the ulcer and blood, and the patient received intravenous penicillin and vancomycin for one week. when sensitivity studies were returned he was treated with gatifloxacin orally. after two weeks of combined antimicrobial therapy and negative blood cultures, the patient r ... | 2005 | 15700434 |
| antibacterial activity of an eudesmane sesquiterpene isolated from common varthemia, varthemia iphionoides. | the ethyl acetate extract of the whole aerial parts of varthemia iphionoides (compositae) showed a pronounced antibacterial activity. the bioassay-guided fractionation of the ethyl acetate extract and further purification of the most antibacterially active fraction led to the isolation and identification of an antibacterial sesquiterpene, selina-4,11(13)-dien-3-on-12-oic acid. this compound exhibited potent antimicrobial activity against six bacterial species (staphylococcus aureus, bacillus sub ... | 2004 | 15707759 |
| identification of an antifungal chitinase from a potential biocontrol agent, bacillus cereus 28-9. | bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in taiwan. this bacterium exhibited biocontrol potential on botrytis leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. at least two chitinases (chicw and chich) were excreted by b. cereus 28-9. the chicw-encoding gene was cloned and moderately expressed in escherichia coli dh5alpha. near homogenous chicw was obtained from the periplasmic fraction of e. coli cells harboring chicw by a puri ... | 2005 | 15715951 |
| presence of bacillus cereus in street foods in gaborone, botswana. | the purpose of this study was to evaluate the microbiological safety and quality of street foods sold in gaborone, botswana. a total of 148 point-of-sale composite street food samples were bought and analyzed between june 2001 and may 2002. the analysis focused on the level of contamination of various street foods with bacillus cereus. the b. cereus (vegetative and spores), total spore, and total viable counts were determined on all the samples. also b. cereus isolates from 444 individual point- ... | 2005 | 15726979 |
| gil16, a new gram-positive tectiviral phage related to the bacillus thuringiensis gil01 and the bacillus cereus pbclin15 elements. | one of the most notable characteristics of tectiviridae resides in their double-layer coats: the double-stranded dna is located within a flexible lipoprotein vesicle covered by a rigid protein capsid. despite their apparent rarity, tectiviruses have an extremely wide distribution compared to other phage groups. members of this family have been found to infect gram-negative (prd1 and relatives) as well as gram-positive (bam35, gil01, ap50, and phins11) hosts. several reports have shown that tecti ... | 2005 | 15743944 |
| toxin-producing ability among bacillus spp. outside the bacillus cereus group. | a total of 333 bacillus spp. isolated from foods, water, and food plants were examined for the production of possible enterotoxins and emetic toxins using a cytotoxicity assay on vero cells, the boar spermatozoa motility assay, and a liquid chromatography-mass spectrometry method. eight strains produced detectable toxins; six strains were cytotoxic, three strains produced putative emetic toxins (different in size from cereulide), and one strain produced both cytotoxin(s) and putative emetic toxi ... | 2005 | 15746316 |
| fingerprinting of bacillus thuringiensis type strains and isolates by using bacillus cereus group-specific repetitive extragenic palindromic sequence-based pcr analysis. | a total of 119 bacillus thuringiensis strains (83 type strains and 26 native isolates), as well as five b. cereus group species, were analyzed by repetitive extragenic palindromic sequence-based pcr analysis (rep-pcr) fingerprinting. primers bc-rep-1 and bc-rep-2 were specifically designed according to an extragenic 26-bp repeated sequence found in the six b. cereus group genomes reported. a total of 47 polymorphic bands were detected, and the patterns varied from 5 to 13 bands in number and fro ... | 2005 | 15746337 |
| polysaccharide-producing bacteria isolated from paper machine slime deposits. | development of novel enzymatic methods for slime deposit control in paper mills requires knowledge of polysaccharide-producing organisms and the polysaccharide structures present in deposits. in this work, 27 polysaccharide-producing bacteria were isolated from slime samples collected from different parts of a paper machine. most of the isolates produced polysaccharides in liquid culture and nine of them were selected for production of polysaccharides for characterisation. the selected isolates ... | 2005 | 15750806 |
| antagonistic effects of several bacteria on fusarium oxysporum, the causal agent of root and crown rot of onion under field conditions. | onion (allium cepa) is one of the most important vegetable crop which is commonly used as a food supplement. this plant is found to be vulnerable to various pathogenic infections during its growth development. among different onion diseases, root and crown rot,caused by fusarium oxysporum f.sp. cepa, s considered an importantfungal disease. in this study, the inhibitory effect of bacillus cereus (isolates 22 and 52), b. subtilis (isolate 126), pseudomonas fluorescens (isolates 48 and chao), beno ... | 2004 | 15756854 |
| "biocontrol of psychrotrophic enterotoxigenic bacillus cereus in a nonfat hard cheese by an enterococcal strain-producing enterocin as-48," a comment on: j. food prot. 67(7):1517-1521 (2004). | 2005 | 15771164 | |
| bacillus cereus food poisoning and its toxins. | the genus bacillus includes members that demonstrate a wide range of diversity from physiology and ecological niche to dna sequence and gene regulation. the species of most interest tend to be known for their pathogenicity and are closely linked genetically. bacillus anthracis causes anthrax, and bacillus thuringiensis is widely used for its insecticidal properties but has also been associated with foodborne disease. bacillus cereus causes two types of food poisoning, the emetic and diarrheal sy ... | 2005 | 15771198 |
| effect of ph on the active site of an arg121cys mutant of the metallo-beta-lactamase from bacillus cereus: implications for the enzyme mechanism. | the zinc-dependent metallo-beta-lactamases are a group of bacterial enzymes that pose a threat to the future efficacy of present-day antibiotics. their mechanism is poorly understood, and there are no clinically useful inhibitors. while most members of the group contain two tightly bound zinc ions in their active sites, the bacillus cereus enzyme has a much lower affinity for its second zinc (zn2), thought to be due to the presence of arg121 immediately beneath the floor of the active site (cf. ... | 2005 | 15779910 |
| the antimicrobial activity of extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent. | the antimicrobial activity and the mic values of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent have been investigated against some microorganisms. at least one of the extracts or 3-hydroxyphysodic acid showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus vulgaris, salmonella ... | 2005 | 15787241 |
| eremophilane-type sesquiterpene derivatives from senecio aegyptius var. discoideus. | investigation of a ch(2)cl(2) extract of the aerial parts of senecio aegyptius var. discoideus afforded nine eremophilane compounds, of which six are new (1-6), namely, 1beta-hydroxy-8alphah-eremophil-7(11),9-dien-8beta,12-olide (1), 1beta,8alpha-dihydroxyeremophil-7(11),9-dien-8beta,12-olide (2), 1beta-hydroxy-8alpha-methoxyeremophil-7(11),9-dien-8beta,12-olide (3), 1-oxo-8alpha-methoxy-10alphah-eremophil-7(11)-en-8beta,12-lactam (4), 1beta,10beta-epoxy-8alpha-hydroxyeremophil-7(11)-en-8beta,12 ... | 2005 | 15787455 |
| genetic diversity among bacillus anthracis, bacillus cereus and bacillus thuringiensis strains using repetitive element polymorphism-pcr. | repetitive element polymorphism-pcr (rep-pcr) is one of the tools that has been used to elucidate genetic diversity of related microorganisms. using the mb1 primer, rep-pcr fingerprints from 110 bacillus strains within the "b. cereus group" have identified eighteen distinct categories, while other more distantly related bacterial species fell within six additional categories. all bacillus anthracis strains tested were found to be monomorphic by fluorophore-enhanced rep-pcr (ferp) fingerprinting ... | 2004 | 15790070 |
| characteristics of bacterial strains inhabiting the wood of coniferous trees. | the presented studies embraced samples of wood chips from coniferous trees which contained layers of duramen, alburnum and bark. microbiological analysis involved qualitative and quantitative determination of bacterial flora inhabiting the studied wood material. the wood chips were found to contain primarily species belonging to the genera bacillus and pseudomonas. the presence of the potentially pathogenic species bacillus cereus 1, sphingomonas paucimobilis, aeromonas salmonicida and chryseomo ... | 2004 | 15790079 |
| multilocus sequence typing reveals that bacillus cereus strains isolated from clinical infections have distinct phylogenetic origins. | eight strains of bacillus cereus isolated from bacteremia and soft tissue infections were assigned to seven sequence types (sts) by multilocus sequence typing (mlst). two strains from different locations had identical sts. the concatenated sequences of the seven sts were aligned with 65 concatenated sequences from reference sts and a neighbor-joining tree was constructed. two strains were distantly related to all reference sts. three strains were recovered in a clade that included bacillus anthr ... | 2005 | 15796996 |
| chitooligosaccharides--preparation with the aid of pectinase isozyme from aspergillus niger and their antibacterial activity. | an isozyme of pectinase from aspergillus niger with polygalacturonase activity caused chitosanolysis at ph 3.5, resulting in low-molecular weight chitosan (86%), chitooligosaccharides (cos, 4.8%) and monomers (2.2%). hplc showed the presence of cos with dp ranging from 2 to 6. charcoal-celite chromatography and re-n-acetylation of the cos followed by cd, ir, maldi-tof-ms and fab-ms analyses revealed an abundance of chitobiose, chitotriose and chitotetraose. the cos-monomeric mixture showed a bac ... | 2005 | 15797142 |
| activation of sphingomyelinase from bacillus cereus by zn2+ hitherto accepted as a strong inhibitor. | sphingomyelinase (smase) from bacillus cereus has been known to be activated by mg2+, mn2+, and co2+, but strongly inhibited by zn2+. in the present study, we investigated the effects of several kinds of metal ions on the catalytic activity of b. cereus smase, and found that the activity was inhibited by zn2+ at its higher concentrations or at higher ph values, but unexpectedly activated at lower zn2+ concentrations or at lower ph values. this result indicates that smase possesses at least two d ... | 2005 | 15797235 |
| finding optimal photosensitisers for the decontamination of foods by the photodynamic effect. | a new method for sanitation based on the photodynamic effect is introduced. photosensitisers are compounds that generate cytotoxic oxygen species and free radicals after excitation with appropriate visible light. the cell killing efficacy is due to their chemical structure, to the actual environment and therefore also to their degree of aggregation. as has been shown, photosensitisers bound to the cell wall or incorporated into the cells are very effective cytotoxic agents after illumination. ev ... | 2003 | 15806940 |
| synthesis of sphingomyelin sulfur analogue and its behavior toward sphingomyelinase. | the sulfur analogue of sphingomyelin was designed and stereoselectively synthesized from s-benzyl-n-boc-cysteine. the introduction of the phosphoryl choline moiety was successfully achieved by our own method using 2-bromoethyl dimethyl phosphite and carbon tetrabromide followed by a trimethylamine treatment. the synthesized compound proved to be a useful substrate for monitoring the enzyme activity of sphingomyelinase by detecting the liberated thiol group with a thiol-sensitive reagent. | 2005 | 15808485 |
| genomics of the bacillus cereus group of organisms. | members of the bacillus cereus group of organisms include bacillus cereus, bacillus anthracis and bacillus thuringiensis. collectively, these organisms represent microbes of high economic, medical and biodefense importance. given this significance, this group contains the highest number of closely related fully sequenced genomes, giving the unique opportunity for thorough comparative genomic analyses. much of the disease and host specificity of members of this group can be attributed to their pl ... | 2005 | 15808746 |
| molecular recognition of an adp-ribosylating clostridium botulinum c3 exoenzyme by rala gtpase. | c3 exoenzymes (members of the adp-ribosyltranferase family) are produced by clostridium botulinum (c3bot1 and -2), clostridium limosum (c3lim), bacillus cereus (c3cer), and staphylococcus aureus (c3stau1-3). these exoenzymes lack a translocation domain but are known to specifically inactivate rho gtpases in host target cells. here, we report the crystal structure of c3bot1 in complex with rala (a gtpase of the ras subfamily) and gdp at a resolution of 2.66 a. rala is not adp-ribosylated by c3 ex ... | 2005 | 15809419 |
| clinical significance of bacteriologic screening in platelet concentrates. | despite routine bacterial screening with a bacterial culturing system (bact/alert, biomerieux) of platelet (plt) concentrates, two cases of life-threatening sepsis attributed to transfused plt products contaminated with bacillus cereus were reported to the regional hemovigilance office in the southwest region of the netherlands. these reports necessitated a retrospective evaluation of the currently applied bacteriologic screening program. | 2005 | 15819671 |
| an assessment of pasteurization treatment of water, media, and milk with respect to bacillus spores. | this study evaluated the ability of spore-forming bacillus spp. to resist milk pasteurization conditions from 72 to 150 degrees c. spores from the avirulent surrogate sterne strain of bacillus anthracis, as well as a representative strain of a common milk contaminant that is also a pathogen, bacillus cereus atcc 9818, were heated at test temperatures for up to 90 min in dh2o, brain heart infusion broth, or skim milk. in skim milk, characteristic log reductions (log cfu per milliliter) for b. ant ... | 2005 | 15830666 |
| the role of sigmab in the stress response of gram-positive bacteria -- targets for food preservation and safety. | the alternative sigma factor sigmab modulates the stress response of several gram-positive bacteria, including bacillus subtilis and the food-borne human pathogens bacillus cereus, listeria monocytogenes and staphylococcus aureus. in all these bacteria, sigmab is responsible for the transcription of genes that can confer stress resistance to the vegetative cell. recent findings indicate that sigmab also plays an important role in antibiotic resistance, pathogenesis and cellular differentiation p ... | 2005 | 15831390 |
| transfer and expression of the mosquitocidal plasmid pbtoxis in bacillus cereus group strains. | the toxicity of bacillus thuringiensis subsp. israelensis to dipteran larvae (mosquitoes and black flies) depends on the presence of the pbtoxis plasmid. in this paper, two antibiotic resistance tagged pbtoxis were transferred by conjugation to other bacillus cereus group strains. among 15 potential recipients, only a lepidopteran active b. thuringiensis subspecies kurstaki and a b. cereus strain received the plasmid pbtoxis with a low transfer rate of about 10(-8) transconjugants/recipient. the ... | 2005 | 15837378 |
| plasmid-encoded regulator of extracellular proteases in bacillus anthracis. | bacillus anthracis sterne cured of the pxo1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. a zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. there were similar amounts of protease secreted by the closely related species bacillus cereus and bacillus thuringiensis, but the patterns differed. among the pxo1 plasmid-encoded proteins, there is a tetratricopepti ... | 2005 | 15838040 |
| biodegradation of imazapyr by free cells of pseudomonas fluorescene biotype ii and bacillus cereus isolated from soil. | 2005 | 15841977 | |
| production and purification of a calcium-dependent protease from bacillus cereus bg1. | the production and purification of a calcium-dependent protease by bacillus cereus bg1 were studied. the production of the protease was found to depend specifically on the calcium concentration in the culture medium. this suggests that this metal ion is essential for the induction of protease production and/or stabilisation of the enzyme after synthesis. the calcium requirement is highly specific since other metal ions (such as mg(2+) and ba(2+), which both activate the enzyme) are not able to i ... | 2005 | 15843974 |
| grafted thermo- and ph responsive co-polymers: surface-properties and bacterial adsorption. | a series of responsive polymers displaying ph and temperature-mediated phase changes were prepared from n-isopropylacrylamide and omega-carboxylic acid functionalised acrylamides. these polymers were grafted to surfaces and their characteristics probed by atomic force microscopy in aqueous solutions. the effects of ph and temperature induced phase transitions on the short-term adsorption of the bacteria salmonella typhimurium and bacillus cereus from pure cultures were assessed. contact angle st ... | 2005 | 15847993 |
| tnxo1, a germination-associated class ii transposon from bacillus anthracis. | bacillus anthracis harbours two virulence plasmids, pxo1 (182 kb) and pxo2 (95 kb). whereas pxo2 harbours the cap operon coding for the capsule, pxo1 contains the pag, lef, and cya genes coding for protective antigen, lethal, and oedema factors, respectively, as well as the atxa regulatory gene. these genes are located within a 44.8 kb long pathogenicity island flanked by insertion sequences. here, we describe the presence in the same plasmid region of an 8679 bp genetic element displaying the s ... | 2005 | 15848228 |
| use of fatty acid profiles to identify food-borne bacterial pathogens and aerobic endospore-forming bacilli. | capillary gas chromatography (gc) with flame ionization detection was used to determine the cellular fatty acid profiles of various food-borne microbial pathogens and to compare the fatty acid profiles of spores and vegetative cells of the same endospore-forming bacilli. fifteen bacteria, representing eight genera (staphylococcus, listeria, bacillus, yersinia, salmonella, shigella, escherichia, and vibrio) and 11 species were used to compare the extracted fatty acid methyl esters (fames). endosp ... | 2005 | 15853428 |
| temperature effect on bacterial growth rate: quantitative microbiology approach including cardinal values and variability estimates to perform growth simulations on/in food. | temperature effect on growth rates of listeria monocytogenes, salmonella, escherichia coli, clostridium perfringens and bacillus cereus, was studied. growth rates were obtained in laboratory medium by using a binary dilutions method in which 15 optical density curves were generated to determine one mu value. the temperature was in the range from 2 to 48 degrees c, depending on the bacterial species. data were analysed after a square root transformation. no large difference between the strains of ... | 2004 | 15854703 |
| a modified weibull model for bacterial inactivation. | in this paper, a modified weibull model is proposed to fit microbial survival curves. this model can incorporate shoulder and/or tailing phenomena if they are encountered. we aim to obtain an accurate fit of the "primary" modelling of the bacterial inactivation and to provide a useful and meaningful model for biologists and food industry. a delta parameter close to the classical concept of the d value, established for sterilisation processes, is used in the model. the specific parameterisation o ... | 2004 | 15854705 |
| validation of an overall model describing the effect of three environmental factors on the apparent d-value of bacillus cereus spores. | several factorial models extending the famous bigelow model to describe the influence of the heating and recovery ph and a(w) conditions on bacterial heat resistance have been developed. these models can be associated in an overall multifactorial model describing the influences of heating and recovery conditions on d values. for bacillus cereus strain adqp 407 the model parameters characterising the environmental factor influences (ph, temperature, a(w)) were evaluated. determination of bacteria ... | 2005 | 15854707 |
| [isolation and study of azobenzene converting soil bacteria]. | heterotrophic bacteria were isolated from soil and glass slides and classified as bacillus cereus snk12, paenibacillus polymyxa snk2, azotobacter chroococcum ankii, and ochrobacterium intermedium anki. their cultures could degrade azobenzene under the conditions of co-metabolism. a rapid test for the ability of bacteria to convert azobenzenes is proposed. | 2005 | 15859461 |
| the linear double-stranded dna of phage bam35 enters lysogenic host cells, but the late phage functions are suppressed. | bam35, a temperate double-stranded dna bacteriophage with a 15-kb linear genome, infects gram-positive bacillus thuringiensis cells. bam35 morphology and genome organization resemble those of prd1, a lytic phage infecting gram-negative bacteria. bam35 and prd1 have an outer protein coat surrounding a membrane that encloses the viral dna. we used electrochemical methods to investigate physiological changes of the lysogenic and nonlysogenic hosts during bam35 dna entry and host cell lysis. during ... | 2005 | 15866940 |
| use of a quartz crystal microbalance to investigate the antiadhesive potential of n-acetyl-l-cysteine. | the reduction of bacterial biofilm formation on stainless steel surfaces by n-acetyl-l-cysteine (nac) is attributed to effects on bacterial growth and polysaccharide production, as well as an increase in the wettability of steel surfaces. in this report, we show that nac-coated stainless steel and polystyrene surfaces affect both the initial adhesion of bacillus cereus and bacillus subtilis and the viscoelastic properties of the interaction between the adhered bacteria and the surface. a quartz ... | 2005 | 15870362 |
| practical application of dynamic temperature profiles to estimate the parameters of the square root model. | optimal experimental design for parameter estimation (oed/pe) is a promising method to improve parameter estimation accuracy and minimise experimental effort in the field of predictive microbiology. in this paper, the oed/pe methodology was applied on two practical examples: the growth of bacillus cereus and enterobacter cloacae in liquid whole egg product. both strains were recovered from samples of a commercial product. the goal of the modelling exercise was to quantify the influence of temper ... | 2005 | 15878409 |
| premicellar complexes of sphingomyelinase mediate enzyme exchange for the stationary phase turnover. | during the steady state reaction progress in the scooting mode with highly processive turnover, bacillus cereus sphingomyelinase (smase) remains tightly bound to sphingomyelin (sm) vesicles (yu et al., biochim. biophys. acta 1583, 121-131, 2002). in this paper, we analyze the kinetics of smase-catalyzed hydrolysis of sm dispersed in diheptanoylphosphatidyl-choline (dc7pc) micelles. results show that the resulting decrease in the turnover processivity induces the stationary phase in the reaction ... | 2005 | 15878423 |
| [heat treatment for the control of bacillus cereus spores in foods]. | the effect of heat treatments on food products related to soybean curd contaminated with bacillus cereus spores was investigated for the purpose of preventing food-poisoning outbreaks by b. cereus. in the case of b. cereus strains isolated from foods, heating foods inoculated with the spores for 20 min at 70 degrees c, 5 min at 75 degrees c, 2 min at 100 degrees c, or 10 sec with a microwave oven plus reheating after standing for 2 hr at 25 degrees c reduced the number of surviving cells within ... | 2005 | 15881248 |
| a ferric dicitrate uptake system is required for the full virulence of bacillus cereus. | bacillus cereus is an opportunistic human pathogen of increasing prevalence. analysis of the bacillus cereus genome sequence identified a potential ferric dicitrate uptake system. the three-gene operon was confirmed to be negatively regulated by the ferric uptake repressor (fur). the fec operon was genetically silenced using the integration suicide vector pmutin4. the mutant strain displayed no growth defect under iron-limited conditions but was unable to grow on ferric citrate as a sole iron so ... | 2005 | 15886918 |
| necrotizing infection due to bacillus cereus mimicking gas gangrene following penetrating trauma. | an 8-year-old boy presented with fulminant necrotizing infection resembling gas gangrene following penetrating trauma from a tree branch. bacillus cereus was isolated from tissue specimens, showing that unexpected pathogens can be isolated. it is essential to submit specimens for culture, as this organism is typically resistant to beta-lactam antibiotics and metronidazole, the empiric choice for gas gangrene. | 2005 | 15891547 |
| host-pathogen interactions: a proteomic view. | host-pathogen interactions reflect the balance of host defenses and pathogen virulence mechanisms. advances in proteomic technologies now afford opportunities to compare protein content between complex biologic systems ranging from cells to animals and clinical samples. thus, it is now possible to characterize host-pathogen interactions from a global proteomic view. most reports to date focus on cataloging protein content of pathogens and identifying virulence-associated proteins or proteomic al ... | 2005 | 15892564 |
| the exsa protein of bacillus cereus is required for assembly of coat and exosporium onto the spore surface. | the outermost layer of spores of the bacillus cereus family is a loose structure known as the exosporium. spores of a library of tn917-ltv1 transposon insertion mutants of b. cereus atcc 10876 were partitioned into hexadecane; a less hydrophobic mutant that was isolated contained an insertion in the exsa promoter region. exsa is the equivalent of safa (yrba) of bacillus subtilis, which is also implicated in spore coat assembly; the gene organizations around both are identical, and both proteins ... | 2005 | 15901704 |
| successful treatment of bacillus cereus meningitis following allogenic stem cell transplantation. | we report the case of a 19-yr-old boy, who received an allogeneic stem cell transplantation for the second relapse of hodgkin's disease. the patient developed seizures and flaccid hemiparesis on day +10. meningoencephalitis induced by bacillus cereus was diagnosed. the treatment consisted of appropriate antibiotics, g-csf and removal of the central venous line. infection control and nearly full neurological recovery was achieved. immunocompromised patients susceptible to b. cereus infection, ind ... | 2005 | 15910391 |
| bacterial strains from moldy buildings are highly potent inducers of inflammatory and cytotoxic effects. | we aimed to identify inflammatory and cytotoxic potential of individual indoor air bacterial and fungal strains, as well as extracts of indoor air filter samples containing bacteria and fungi. mouse raw264.7 macrophages were exposed in vitro to four bacterial strains; streptomyces californicus, mycobacterium terrae, bacillus cereus and pseudomonas fluorescens, and three fungal strains; penicillium spinulosum, aspergillus versicolor and stachybotrys chartarum. furthermore, raw264.7 macrophages we ... | 2005 | 15910531 |
| dynamic kinetic resolution of amino acid amide catalyzed by d-aminopeptidase and alpha-amino-epsilon-caprolactam racemase. | amino acid amide racemizing activity was discovered in alpha-amino-epsilon-caprolactam (acl) racemase (ec 5. 1. 1. 15) from achromobacter obae. the enzymatic synthesis of d-alanine from l-alanine amide has been demonstrated by use of d-aminopeptidase (dap; ec 3. 4. 11. 19) from ochrobactrum anthropi c1-38 and acl racemase. the conversion of 45 mm l-alanine amide was carried out at 30 degrees c for 7 h; l-alanine amide was completely converted to d-alanine, and no l-alanine was detected. the resu ... | 2005 | 15913357 |
| partial purification and characterization of protease enzyme from bacillus subtilis and bacillus cereus. | the aim of this experimental study was to isolate and partially purify protease enzyme from bacillus cereus and bacillus subtilis. protease enzyme is obtained by inducing spore genesis of bacteria from bacillus species in suitable nutrient plates. the partial purification was realized by applying, respectively, ammonium sulfate precipitation, dialysis, and deae-cellulose ion-exchange chromatography to the supernatant that was produced later. optimum ph, optimum temperature, ph stability, and tem ... | 2005 | 15917598 |
| microbial synthesis and characterization of physiochemical properties of polyhydroxyalkanoates (phas) produced by bacteria isolated from activated sludge obtained from the municipal wastewater works in hong kong. | the first objective of this study was the measurement of physical properties of p(3hb-co-3hv) copolymers with different (hydroxybutyrate) hb to (hydroxyvalerate) hv ratios produced by bacillus cereus (try2) isolated from activated sludge. the 3hv phbv copolymers were 0.05, 22.6, 39.2, 54.1, and 69.1 mol%, respectively. the second objective was to study possible waste water treatment and production of phas at the same time by b. cereus (try2) and pseudomonas spp. (tob17) (both were isolated from ... | 2005 | 15920276 |
| hydrocarbon biodegradation in oxygen-limited sequential batch reactors by consortium from weathered, oil-contaminated soil. | we studied the use of sequential batch reactors under oxygen limitation to improve and maintain consortium ability to biodegrade hydrocarbons. air-agitated tubular reactors (2.5 l) were operated for 20 sequential 21-day cycles. maya crude oil-paraffin mixture (13,000 mg/l) was used as the sole carbon source. the reactors were inoculated with a consortium from the rhizosphere of cyperus laxus, a native plant that grows naturally in weathered, contaminated soil. oxygen limitation was induced in th ... | 2005 | 15920621 |
| formation of the spore clumps during heat treatment increases the heat resistance of bacterial spores. | effects of the clumping of bacterial spores on their heat resistance as a result of heat treatment were investigated. spore suspensions of bacillus cereus, bacillus coagulans and bacillus licheniformis were heated at 85 degrees c. survivor curves of the three strains showed tailing in all treatments after 30 min. as the treatment time increased, the formation of spore clumps increased in all strains after 20 min. relative hydrophobicity of the spore surface increased as a result of heat treatmen ... | 2005 | 15925006 |
| chromium-resistant bacteria and cyanobacteria: impact on cr(vi) reduction potential and plant growth. | two chromium-resistant bacterial strains, bacillus cereus s-6 and ochrobactrum intermedium crt-1, and two cyanobacterial strains, oscillatoria sp. and synechocystis sp., were used in this study. at initial chromate concentrations of 300 and 600 microg k2cro4 ml(-1), and an inoculum size of 9.6 x 10(7) cells ml(-1), b. cereus s-6 completely reduced cr(vi), while o. intermedium crt-1 reduced cr(vi) by 98% and 70%, respectively after 96 h. at 100 microg k2cro4 ml(-1), synechocystis sp. mk(s) and os ... | 2005 | 15926088 |
| production of catechol-siderophore and utilization of transferrin-bound iron in bacillus cereus. | in the present study we attempted to ascertain whether bacillus cereus was able to produce catechol-siderophore(s), and whether it was able to utilize transferrin-bound iron. the growth of b. cereus was stimulated in proportion to the iron-saturation level of the transferrin, and catechol-siderophores were produced in inverse proportion to this level. b. cereus was proved to uptake iron from partially iron-saturated transferrin or holotransferrin, without destroying the transferrin by its protea ... | 2005 | 15930764 |
| characterization of chito-oligosaccharides prepared by chitosanolysis with the aid of papain and pronase, and their bactericidal action against bacillus cereus and escherichia coli. | papain (from papaya latex; ec 3.4.22.2) and pronase (from streptomyces griseus; ec 3.4.24.31) caused optimum depolymerization of chitosan at ph 3.5 and 37 degrees c, resulting in lmmc (low molecular mass chitosan) and chito-oligomeric-monomeric mixture. the yield of the latter was 14-16% and 14-19% respectively for papain- and pronase-catalysed reactions, depending on the reaction time (1-5 h). hplc revealed the presence of monomer(s) and oligomers of dp (degree of polymerization) 2-6, which was ... | 2005 | 15932346 |
| influence of glutamate on growth, sporulation, and spore properties of bacillus cereus atcc 14579 in defined medium. | a chemically defined medium in combination with an airlift fermentor system was used to study the growth and sporulation of bacillus cereus atcc 14579. the medium contained six amino acids and lactate as the main carbon sources. the amino acids were depleted during exponential growth, while lactate was metabolized mainly during stationary phase. two concentrations of glutamate were used: high (20 mm; ylhg) and low (2.5 mm; yllg). under both conditions, sporulation was complete and synchronous. s ... | 2005 | 15933027 |
| phosphatidylinositol-specific phospholipase c of bacillus anthracis down-modulates the immune response. | phosphatidylinositol-specific phospholipases (pi-plcs) are virulence factors produced by many pathogenic bacteria, including bacillus anthracis and listeria monocytogenes. bacillus pi-plc differs from listeria pi-plc in that it has strong activity for cleaving gpi-anchored proteins. treatment of murine dcs with bacillus, but not listeria, pi-plc inhibited dendritic cell (dc) activation by tlr ligands. infection of mice with listeria expressing b. anthracis pi-plc resulted in a reduced ag-specifi ... | 2005 | 15944308 |
| expanding the bactericidal action of the food color additive phloxine b to gram-negative bacteria. | phloxine b (d&c red no. 28) is a color additive for food, drugs, and cosmetics. it has been previously shown to have anti-staphylococcus aureus activities. in this work, the effect of phloxine b on various gram-negative bacteria and other gram-positive bacteria including bacillus cereus, bacillus thuringiensis, bacillus mycoides, bacillus subtilis, bacillus aureus, salmonella, escherichia coli and shigella was studied, along with the mechanism of anti-microbial activity. in the presence of fluor ... | 2005 | 15949926 |
| inhibitors of metallo-beta-lactamase generated from beta-lactam antibiotics. | the resistance of bacteria to the normally lethal action of beta-lactam antibiotics is largely due to the production of beta-lactamases that catalyze the hydrolysis of the beta-lactam. one class of these enzymes is a zinc-dependent metallo-beta-lactamase for which there are no clinically available inhibitors. the hydrolysis of cephalosporin beta-lactam antibiotics generates dihydrothiazines which subsequently undergo isomerization at c6 by c-s bond cleavage and through the intermediacy of a thio ... | 2005 | 15952764 |
| spore-formers in foods and the food processing chain. | 2001 | 15954647 | |
| antimicrobial activity of lactic acid bacteria isolated from tenerife cheese: initial characterization of plantaricin tf711, a bacteriocin-like substance produced by lactobacillus plantarum tf711. | the screening and initial characterization of bacteriocins produced by lactic acid bacteria (lab) from raw tenerife goats' cheese with possible application as biopreservatives or ripening accelerators for tenerife cheese. | 2005 | 15960667 |
| aerosolization as novel sanitizer delivery system to reduce food-borne pathogens. | as a preliminary experiment on new sanitizer delivery tools, the efficacy of aerosolized sanitizer on food-borne pathogens was investigated in larger model chamber system. | 2005 | 15960753 |
| peptidoglycan n-acetylglucosamine deacetylases from bacillus cereus, highly conserved proteins in bacillus anthracis. | the genomes of bacillus cereus and its closest relative bacillus anthracis contain 10 polysaccharide deacetylase homologues. six of these homologues have been proposed to be peptidoglycan n-acetylglucosamine deacetylases. two of these genes, namely bc1960 and bc3618, have been cloned and expressed in escherichia coli, and the recombinant enzymes have been purified to homogeneity and further characterized. both enzymes were effective in deacetylating cell wall peptidoglycan from the gram(+) bacil ... | 2005 | 15961396 |
| rare trisubstituted sesquiterpenes daucanes from the wild daucus carota. | phytochemical and biological investigation of the roots of the wild daucus carota ssp. carota afforded three new and four known compounds, including four sesquiterpenes daucane esters (1-3 [new], and 4), one polyacetylene (5), one sesquiterpene coumarin (6), and sitosterol glucoside. the structures of the new compounds were determined by comprehensive nmr studies, including dept, cosy, noesy, hmqc and hmbc analyses. based on an agar diffusion assay, 1, 2 and 4-6 were screened and found to contai ... | 2005 | 15964039 |
| in-vitro antibacterial, antifungal and cytotoxic properties of sulfonamide--derived schiff's bases and their metal complexes. | a series of new antibacterial and antifungal schiff's bases derived from sulfonamides, as well as their transition metal complexes incorporating cobalt (ii), copper (ii), nickel (ii) and zinc (ii) were synthesized, characterized and screened for their in-vitro antibacterial activity against six gram-negative (escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi and shigella dysentriae) and four gram-positive (bacillus cereus, corynebacterium diphth ... | 2005 | 15968823 |
| use of a spectrophotometric bioassay for determination of microbial sensitivity to manuka honey. | the antimicrobial activity of manuka honey has been well documented (molan, 1992a,b,c, 1997) [molan, p.c., 1992. the antibacterial activity of honey. 1: the nature of the antibacterial activity. bee world 73 (1) 5-28; molan, p.c., 1992. the antibacterial activity of honey. 2: variation in the potency of the antibacterial activity. bee world 73 (2) 59-76; molan, p.c., 1992. medicinal uses for honey. beekeepers quarterly 26; molan, p.c., 1997. finding new zealand honeys with outstanding antibacter ... | 2006 | 15979745 |
| in-house validation of the bactec 9240 blood culture system for detection of bacterial contamination in platelet concentrates. | at present, only two commercially available automated culture systems are cleared by the fda for the purpose of quality control (qc) testing for bacterial contamination of platelet (plt) concentrates: the bact/alert blood culture system (biomérieux) and the pall ebds (pall corporation), both of which allow testing of leukoreduced apheresis as well as whole blood-derived plts. after the decision of the aabb to institute universal qc testing of plt concentrates for evidence of bacterial contaminat ... | 2005 | 15987359 |
| [identification of psychrotrophs syp-a2-3 producing cold-adapted protease from the no. 1 glacier of china and study on its fermentation conditions]. | the psychrotrophs syp-a2-3 producing the cold-adapted protease has been isolated from the bacterial samples collected from the no. 1 glacier of china and identified as bacillus cereus according to its morphological and physiochemical characteristics and 16s rdna gene sequence analysis. it could grow between 0 degree c and 38 degrees c while its optimal growth temperature was 25 degrees c and the optimal temperature for its protease production was 15 degrees c. the cold-adapted protease was ident ... | 2005 | 15989272 |
| effect of the implementation of haccp on the microbiological quality of meals at a university restaurant. | a study was conducted to evaluate the microbiological quality, including total mesophilic counts and markers of bacteriological hygiene, as indicator of food safety of three categories of the most consumed meals in a university restaurant, before and after implementation of the haccp system and personnel training. cold gastronomy products, cooked warm-served products, and cooked cold-served products were tested for bacterial contamination. throughout the experiment, 894 samples were examined for ... | 2005 | 15992308 |
| antagonistic activity against salmonella infection in vitro and in vivo for two lactobacillus strains from swine and poultry. | in this study, we isolated two lactobacillus strains, i.e., strain lap5 and lf33, from swine and poultry, respectively, and showed that both strains were acid as well as bile tolerant and were able to adhere to the cultured human intestinal cell lines, such as int-407 and caco-2 cells, and to the intestinal epithelium cells isolated from swine, poultry and mouse (balb/c). both of these lab strains were shown to inhibit the growth of escherichia coli, salmonella typhimurium, staphylococcus aureus ... | 2005 | 15992617 |