Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [acute measenteric adenitis due to pasteurella pseudotuberculosis (malassez-vignal bacillus). apropos of 30 observations. 1. clinical serological and bacteriological study]. | 1960 | 14423389 | |
| [studies with pasteurella pseudotuberculosis and pasteurella pestis phages]. | 1962 | 14457197 | |
| [mesenteric adenitis due to pasteurella pseudotuberculosis]. | 1962 | 14462654 | |
| [on the epidemiology of pasteurella pseudotuberculosis infection in man]. | 1962 | 14471487 | |
| [on the "appendicitic" form of pasteurella pseudotuberculosis infection in children]. | 1962 | 14475993 | |
| [on a case of re-laparotomy after an appendicitic form of course of pasteurella pseudotuberculosis]. | 1961 | 14484466 | |
| family outbreak of pasteurella pseudotuberculosis infection. | 1962 | 14490225 | |
| comparison of the structure and regulation of the udp gene of vibrio cholerae, yersinia pseudotuberculosis, salmonella typhimurium, and escherichia coli. | the nucleotide sequences of the udp gene encoding uridine phosphorylase of yersinia pseudotuberculosis and vibrio cholerae are presented and compared with the udp sequences of salmonella typhimurium and escherichia coli. both genes contain 759 bases and encode a 253 amino acid polypeptide, which is the same as for e. coli and s. typhimurium. the amino acid sequence derived from s. typhimurium gene was more similar to the derived e. coli sequence, with only a 7 amino acid difference. the y. pseud ... | 2003 | 14499937 |
| the ability to replicate in macrophages is conserved between yersinia pestis and yersinia pseudotuberculosis. | yersinia pestis, the agent of plague, has arisen from a less virulent pathogen, yersinia pseudotuberculosis, by a rapid evolutionary process. although y. pestis displays a large number of virulence phenotypes, it is not yet clear which of these phenotypes descended from y. pseudotuberculosis and which were acquired independently. y. pestis is known to replicate in macrophages, but there is no consensus in the literature on whether y. pseudotuberculosis shares this property. we investigated wheth ... | 2003 | 14500510 |
| pseudotuberculosis in sheep as a concern of veterinary public health. | 2003 | 14535419 | |
| ileitis due to yersinia pseudotuberculosis in crohn's disease. | we present the case of a young woman with fulminating terminal ileitis due to yersinia pseudotuberculosis, in whom crohn's disease was subsequently diagnosed. serological testing was prompted by radiological, macroscopic and histological changes which were atypical of crohn's disease. we speculate that y. pseudotuberculosis ileitis may occasionally produce an acute exacerbation of crohn's disease, but that its occurrence may not be diagnosed if the atypical features are not recognised. in this c ... | 2003 | 14556758 |
| a caenorhabditis elegans model of yersinia infection: biofilm formation on a biotic surface. | to investigate yersinia pathogenicity and the evolutionary divergence of the genus, the effect of pathogenic yersiniae on the model organism caenorhabditis elegans was studied. three strains of yersinia pestis, including a strain lacking pmt1, caused blockage and death of c. elegans; one strain, lacking the haemin storage (hms) locus, caused no effect. similarly, 15 strains of yersinia enterocolitica caused no effect. strains of yersinia pseudotuberculosis showed different levels of pathogenicit ... | 2003 | 14600234 |
| use of o-antigen gene cluster-specific pcrs for the identification and o-genotyping of yersinia pseudotuberculosis and yersinia pestis. | yersinia pestis is a very recently evolved clone of yersinia pseudotuberculosis serotype o:1b. this close relationship causes potential difficulties in dna-based diagnostic methods. analysis of the o-antigen gene clusters in these two organisms identified two regions that were used to specifically identify y. pestis-y. pseudotuberculosis as a group or y. pestis alone. both pcr assays were found to be 100% specific when tested on a large collection of yersinia species and other enterobacteriaceae ... | 2003 | 14605146 |
| duplex real-time sybr green pcr assays for detection of 17 species of food- or waterborne pathogens in stools. | a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using the qiaamp dna stool mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. the protocol used the same lc-pcr with 20 pairs of specific primers. the products formed were identified based on a melting point temperature (t(m)) curve analysis. the 17 species of food- or waterborne pathogens examined were enteroinvasive escherichi ... | 2003 | 14605150 |
| interaction of porin from yersinia pseudotuberculosis with different structural forms of endogenous lipopolysaccharide. | the interaction of yersinia pseudotuberculosis porin solubilized in deoxycholate with the s- and r-forms of endogenous lipopolysaccharide (lps) was studied by the quenching of intrinsic protein fluorescence. the samples of s-lps differed both in the length of o-specific polysaccharide (n = 1 and 4) and in the acylation degree of the 3-hydroxytetradecanoic acid residues of the lipid a moiety (12-66%). r-lps (12%) binding to porin was found to occur with positive cooperativity on two integrated st ... | 2003 | 14606939 |
| [mesenteric adenitis and terminal ileitis due to yersinia pseudotuberculosis simulating an abdominal tumor]. | 2003 | 14613698 | |
| septicemia due to yersinia pseudotuberculosis--a case report. | a case report is presented concerning yersinia pseudotuberculosis septicemia presenting as an acute abdominal emergency in an elderly diabetic man with multiple medical problems. | 2003 | 14616729 |
| experimental infections with wild and mutant yersinia pseudotuberculosis strains in rabbits. | experimental oral infections of rabbits with a wild-type yersinia pseudotuberculosis strain (pib102), and two null-mutants (yopk and ypka) were carried out with the aim to explore the possibility to use mutant strains of y. pseudotuberculosis as live carrier vaccine strains. the infectious process of the three strains proceed with passing hyperthermia, leucocytosis with granulocytosis, moderate monocytosis and a transient lymphopenia, better demonstrated at mutant strain infections. short-term b ... | 2003 | 14628999 |
| lack of o-antigen is essential for plasminogen activation by yersinia pestis and salmonella enterica. | the o-antigen of lipopolysaccharide (lps) is a virulence factor in enterobacterial infections, and the advantage of its genetic loss in the lethal pathogen yersinia pestis has remained unresolved. y. pestis and salmonella enterica express beta-barrel surface proteases of the omptin family that activate human plasminogen. plasminogen activation is central in pathogenesis of plague but has not, however, been found to be important in diarrhoeal disease. we observed that the presence of o-antigen re ... | 2004 | 14651623 |
| a convenient synthesis of gdp d-glycero-alpha-d-manno-heptopyranose. | gdp d-glycero-alpha-d-manno-heptopyranose has been prepared in good overall yield from 2,3,4,6,7-penta-o-acetyl-d-glycero-d-manno-heptopyranose by a short-step synthesis. phosphitylation using the phosphoramidite procedure afforded the alpha-anomer in high selectivity. subsequent oxidation and partial deprotection gave the acetylated phosphate derivative, which was subjected to the coupling reaction with gmp-morpholidate to furnish the acetylated heptose nucleoside diphosphate in good yield. de- ... | 2004 | 14659681 |
| the biosynthesis and biological role of lipopolysaccharide o-antigens of pathogenic yersiniae. | lipopolysaccharide (lps) is the major component of the outer leaflet of the outer membrane of gram-negative bacteria. the lps molecule is composed of two biosynthetic entities: the lipid a--core and the o-polysaccharide (o-antigen). most biological effects of lps are due to the lipid a part, however, there is an increasing body of evidence indicating that o-antigen (o-ag) plays an important role in effective colonization of host tissues, resistance to complement-mediated killing and in the resis ... | 2003 | 14670713 |
| [development of a competitive immunoassay based on monoclonal antibodies for the detection of specific antibodies to pseudotuberculosis pathogen]. | a highly sensitive, effective and rapid method--a competitive alternative to elisa--was developed for the detection of specific antibodies to y. pseudotuberculosis. it is based on monoclonal antibodies (mabs) recognizing the species- or serogroup specific epitopes of y. pseudotuberculosis. the competitive elisa was used in testing the murine hyperimmune sera and human antisera sampled from patients with different infectious intestinal diseases including several cases of pseudotuberculosis. the u ... | 2003 | 14708373 |
| [dna microarray for the detection of yersinia pesits]. | to develop a dna microarray technique for fast diagnosis of plague. | 2004 | 14724095 |
| colonic bacterial superantigens evoke an inflammatory response and exaggerate disease in mice recovering from colitis. | there is renewed interest in commensal bacteria as triggers of idiopathic disease, a concept that is prominent in inflammatory bowel disease (ibd). here the effect of intracolonic instillation of staphylococcus aureus enterotoxin b (seb), a model superantigen (sags: potent t-cell stimuli), into mice was examined. | 2003 | 14724831 |
| crystal and solution structures of a superantigen from yersinia pseudotuberculosis reveal a jelly-roll fold. | superantigens are a class of microbial proteins with the ability to excessively activate t cells by binding to the t cell receptor. the staphylococcal and streptococcal superantigens are closely related in structure and possess an n-terminal domain that resembles an ob fold and a c-terminal domain similar to a beta-grasp fold. yersinia pseudotuberculosis produces superantigens, ypma, ypmb, and ypmc, which have no significant amino acid similarity to other proteins. we have determined the crystal ... | 2004 | 14725774 |
| the mechanism of phagocytic uptake promoted by invasin-integrin interaction. | many pathogenic bacterial species produce factors that promote their internalization by host cells. the crucial components for uptake of one such pathogen, yersinia pseudotuberculosis, have been identified. efficient uptake of this microorganism requires tight binding of the bacterial invasin protein to integrins on the cell surface. internalization also involves coordination of signals responsible for cytoskeletal rearrangements and those involved in receptor-mediated endocytosis. a start is be ... | 1995 | 14732167 |
| the yersinia pseudotuberculosis cytotoxic necrotizing factor (cnfy) selectively activates rhoa. | the cytotoxic necrotizing factors (cnf)1 and cnf2 from pathogenic escherichia coli strains activate rhoa, rac1, and cdc42 by deamidation of gln63 (rhoa) or gln61 (rac and cdc42). recently, a novel cytotoxic necrotizing factor termed cnfy was identified in yersinia pseudotuberculosis strains (lockman, h. a., gillespie, r. a., baker, b. d., and shakhnovich, e. (2002) infect. immun. 70, 2708-2714). we amplified the cnfy gene from genomic dna of y. pseudotuberculosis, cloned and expressed the recomb ... | 2004 | 14761941 |
| a palpable right lower abdominal mass due to yersinia mesenteric lymphadenitis. | infection by yersinia pseudotuberculosis has become of increasing pathological importance. this report describes the case of a 12-year-old female with mesenteric lymphadenitis due to yersinia pseudotuberculosis. the patient presented with fever, abdominal pain, and a palpable right abdominal mass. abdominal ultrasonic imaging and computerized axial tomography (ct) revealed a mass. an exploratory laparotomy was performed, followed by appendectomy and mesenteric lymph node biopsy. the diagnosis of ... | 2004 | 14770325 |
| [the toxin of pasteurella pseudotuberculosis; analogies with the toxin of past. pestis (with reference to a memorandum of a. s. lazarus and m. m. nozawa)]. | 1950 | 14771604 | |
| [the decomposition of urea in ferguson synthetic medium by past. pseudotuberculosis, new reaction for differentiating the etiologic agents of plague and pseudotuberculosis]. | 1950 | 14771613 | |
| [experimental research on a strain of pasteurella pseudotuberculosis isolated from a pigeon]. | 1950 | 14787214 | |
| lysis of pasteurella pseudotuberculosis by bacteriophage. | 1951 | 14832498 | |
| rapid differentiation between pasteurella pestis and pasteurella pseudotuberculosis by action of bacteriophage. | 1951 | 14850752 | |
| salad and pseudoappendicitis: yersinia pseudotuberculosis as a foodborne pathogen. | 2004 | 14976590 | |
| a widespread outbreak of yersinia pseudotuberculosis o:3 infection from iceberg lettuce. | the vehicles and sources of yersinia pseudotuberculosis infection are unknown. in finland, clinical microbiology laboratories routinely report y. pseudotuberculosis isolations and submit isolates for serotype analysis. in october 1998, the number of serotype o:3 infections increased markedly. | 2004 | 14976592 |
| identification of novel virulence-associated genes via genome analysis of hypothetical genes. | the sequencing of bacterial genomes has opened new perspectives for identification of targets for treatment of infectious diseases. we have identified a set of novel virulence-associated genes (vag genes) by comparing the genome sequences of six human pathogens that are known to cause persistent or chronic infections in humans: yersinia pestis, neisseria gonorrhoeae, helicobacter pylori, borrelia burgdorferi, streptococcus pneumoniae, and treponema pallidum. this comparison was limited to genes ... | 2004 | 14977936 |
| critical role of t cell migration in bacterial superantigen-mediated shock in mice. | bacterial superantigens have been implicated in the pathogenesis of several human diseases. among them, toxic shock syndrome (tss) is a prototypic acute intoxication caused by the pyrogenic exotoxin family of superantigens. in this study, we investigated the pathophysiological mechanism of tss using the yersinia pseudotuberculosis-derived mitogen (ypm) and its point mutants. the results indicated that ypm could induce toxic shock in balb/c mice but not in t cell-deficient scid mice. we found tha ... | 2004 | 15003813 |
| crystal structure at 1.8 a resolution of cdp-d-glucose 4,6-dehydratase from yersinia pseudotuberculosis. | cdp-d-glucose 4,6-dehydratase catalyzes the conversion of cdp-d-glucose to cdp-4-keto-6-deoxyglucose in an nad(+)-dependent manner. the product of this conversion is a building block for a variety of primary antigenic determinants in bacteria, possibly implicated directly in reactive arthritis. here, we describe the solution of the high-resolution crystal structure of cdp-d-glucose 4,6-dehydratase from yersinia pseudotuberculosis in the resting state. this structure represents the first cdp nucl ... | 2004 | 15023057 |
| the protein kinase pkr is required for macrophage apoptosis after activation of toll-like receptor 4. | macrophages are pivotal constituents of the innate immune system, vital for recognition and elimination of microbial pathogens. macrophages use toll-like receptors (tlrs) to detect pathogen-associated molecular patterns--including bacterial cell wall components, such as lipopolysaccharide or lipoteichoic acid, and viral nucleic acids, such as double-stranded (ds)rna--and in turn activate effector functions, including anti-apoptotic signalling pathways. certain pathogens, however, such as salmone ... | 2004 | 15029200 |
| the recent emergence of plague: a process of felonious evolution. | yersinia pestis, the causative agent of bubonic plague, evolved from closely related yersinia pseudotuberculosis within the past 20,000 years, an event that corresponds to the end of the last ice age and distribution of homo sapiens throughout the world. y. pseudotuberculosis causes chronic but generally mild enteropathogenic infections whereas plague is the most devastating acute disease experienced by mankind. the very recent evolution of plague from its progenitor assures close genomic homoge ... | 2004 | 15037962 |
| yersinia pseudotuberculosis anti-inflammatory components reduce trinitrobenzene sulfonic acid-induced colitis in the mouse. | rectal instillation of trinitrobenzene sulfonic acid (tnbs) induces acute colitis in the mouse. we tested the efficacy of yersinia pseudotuberculosis anti-inflammatory components in preventing tnbs-triggered colitis. animals were orally inoculated with virulence-attenuated yersinia cells (a phop mutant) prior to tnbs administration. under these experimental conditions, colonic lesions and tumor necrosis factor alpha mrna levels were significantly reduced. | 2004 | 15039377 |
| the yersiniae--a model genus to study the rapid evolution of bacterial pathogens. | yersinia pestis, the causative agent of plague, seems to have evolved from a gastrointestinal pathogen, yersinia pseudotuberculosis, in just 1,500-20,000 years--an 'eye blink' in evolutionary time. the third pathogenic yersinia, yersinia enterocolitica, also causes gastroenteritis but is distantly related to y. pestis and y. pseudotuberculosis. why do the two closely related species cause remarkably different diseases, whereas the distantly related enteropathogens cause similar symptoms? the rec ... | 2003 | 15040180 |
| morphological and immunological characterization of immunostimulatory complexes based on glycoglycerolipids from laminaria japonica. | some physicochemical properties of glycoglycerolipids (monogalactosyldiacylglycerol, digalactosyldiacylglycerol and sulfoquinovosyldiacylglycerol) from the sea algae laminaria japonica, as well as their ability to become incorporate into immunostimulating complexes (iscoms), used as a delivery system of microbial and tumor antigens in vesicular form, were studied. these glycolipids were found to differ essentially in fatty acid composition, unsaturation index and thermotropic behavior. the possi ... | 2004 | 15094848 |
| contrasting contributions of complementarity-determining region 2 and hypervariable region 4 of rat bv8s2+ (vbeta8.2) tcr to the recognition of myelin basic protein and different types of bacterial superantigens. | in experimental autoimmune encephalomyelitis (eae) of lew rats, bv8s2(+) (v(beta)8.2) t cells dominate the rt1b(l)-restricted response to guinea pig myelin basic protein (gpmbp), and respond to the superantigens (sag) staphylococcus enterotoxin c1 (sec1), mycoplasma arthritidis sag (mas) and yersinia pseudotuberculosis mitogen (ypm). t cells expressing the closely related bv8s4 differ from bv8s2 t cells in their response to gpmbp, and the sag sec1 and mas, but not in their response to ypm. the f ... | 2004 | 15096488 |
| effect of bovine lactoferricin on enteropathogenic yersinia adhesion and invasion in hep-2 cells. | bovine lactoferricin, a pepsin-generated antimicrobial peptide from bovine lactoferrin active against a wide range of bacteria, was tested for its ability to influence the adhesion and invasion of yersinia enterocolitica and yersinia pseudotuberculosis in hep-2 cells. the addition of non-cytotoxic and non-bactericidal concentrations of lactoferricin to cell monolayers before infection, under different bacterial growth experimental conditions, was ineffective or resulted in about a 10-fold increa ... | 2004 | 15096550 |
| use of florfenicol in non-human primates. | studies were undertaken to determine if florfenicol, an antimicrobial agent structurally similar to chloramphenicol, could be used as an effective broad spectrum antibiotic for the treatment of bacterial infections in primates. florfenicol was developed as an injectable antibiotic for use in cattle on an every other day dosing schedule. its broad spectrum activity, long duration of action following i.m. administration, and its safety as compared with chloramphenicol made it an attractive antibio ... | 2004 | 15102069 |
| identification of signature genes for rapid and specific characterization of yersinia pestis. | polymerase chain reaction (pcr) amplification of dna-based unique markers, the signature sequences, is ideal for rapid detection and identification of pathogens. we described the discovery of twenty-eight signature genes of yersinia pestis by dna microarray-based comparative genome hybridization in conjunction with pcr validation. three pairs of y. pestis-specific primers designed from signature genes were demonstrated to have the expected specificity to this target bacterium, without cross-reac ... | 2004 | 15107536 |
| adp-ribosylation by exoenzyme t of pseudomonas aeruginosa induces an irreversible effect on the host cell cytoskeleton in vivo. | pseudomonas aeruginosa utilises a type iii secretion system (ttss) to introduce exoenzyme s and exoenzyme t into host cells to subvert host cell signalling and thereby promote infection. in this study, we have employed the heterologous ttss of yersinia to deliver different mutants of exot into hela cells. wild-type exot and exot variants expressing either gap (gtpase activating protein) or adp-ribosyltransferase activity mediated changes in cell morphology, which correlated to disruption of the ... | 2004 | 15109724 |
| yersinia yopj inhibits pro-inflammatory molecule expression in human bronchial epithelial cells. | human bronchial epithelial cell pro-inflammatory molecule expression plays a role in the pathogenesis of airway diseases. we hypothesize that yersinia outer protein-j (yopj), a yersinia virulence effector which inhibits mitogen activated protein (map) kinase kinases (mkks), attenuates epithelial cell pro-inflammatory molecule expression. 16hbe14o-cells were co-transfected with cdnas encoding yersinia pseudotuberculosis yopj or empty vector. expression of yopj reduced activation of extracellular ... | 2004 | 15109931 |
| characterization and utilization of monoclonal antibodies reactive to yersinia pseudotuberculosis. | the 3 murine monoclonal antibodies, yps1, yps2 and yps3 reactive to y. pseudotuberculosis can be stabilized and all were found to be of igg type. monoclonal antibody, yps1, recognized a glycoprotein antigen of the organism with reactivity at the 55-75 kda region, while yps2 and yps3 recognized protein antigens of y. pseudotuberculosis 65 kda and 26-28 kda molecular weight regions, respectively. the specificity of monoclonal antibodies was tested using dot elisa and western blotting with whole ce ... | 2003 | 15115097 |
| loss of srf-3-encoded nucleotide sugar transporter activity in caenorhabditis elegans alters surface antigenicity and prevents bacterial adherence. | during the establishment of a bacterial infection, the surface molecules of the host organism are of particular importance, since they mediate the first contact with the pathogen. in caenorhabditis elegans, mutations in the srf-3 locus confer resistance to infection by microbacterium nematophilum, and they also prevent biofilm formation by yersinia pseudotuberculosis, a close relative of the bubonic plague agent yersinia pestis. we cloned srf-3 and found that it encodes a multitransmembrane hydr ... | 2004 | 15123614 |
| age influences resistance of caenorhabditis elegans to killing by pathogenic bacteria. | caenorhabditis elegans has previously been proposed as an alternative host for models of infectious disease caused by human pathogens. when exposed to some human pathogenic bacteria, the life span of nematodes is significantly reduced. we have shown that mutations in the age-1, and/or age-2 genes of c. elegans, that normally enhance life expectancy, can also increase resistance to killing by the bacterial pathogens pseudomonas aeruginosa, salmonella enterica var. typhimurium, burkholderia cepaci ... | 2004 | 15135534 |
| potential therapeutic applications of recombinant, invasive e. coli. | an invasive escherichia coli expressing the inv gene from yersinia pseudotuberculosis was used as a vector for protein delivery to mammalian epithelial cells. upon incubation with beta1-integrin-expressing mammalian cells, the bacteria are internalized, allowing bacteria-encoded proteins to function from within the mammalian cell. these bacteria are eventually processed in the host phagosome where they are destroyed. expression of listeriolysin o from listeria monocytogenes in the bacterium and ... | 2004 | 15141160 |
| excision of the high-pathogenicity island of yersinia pseudotuberculosis requires the combined actions of its cognate integrase and hef, a new recombination directionality factor. | the yersinia high-pathogenicity island (hpi) encodes the siderophore yersiniabactin-mediated iron uptake system. the hpi of yersinia pseudotuberculosis i has previously been shown to be able to excise precisely from the bacterial chromosome by recombination between the attb-r and attb-l sites flanking the island. however, the nature of the y. pseudotuberculosis hpi excision machinery remained unknown. we show here that, upon excision, the hpi forms an episomal circular molecule. the island thus ... | 2004 | 15165237 |
| variation in lipid a structure in the pathogenic yersiniae. | important pathogens in the genus yersinia include the plague bacillus yersinia pestis and two enteropathogenic species, yersinia pseudotuberculosis and yersinia enterocolitica. a shift in growth temperature induced changes in the number and type of acyl groups on the lipid a of all three species. after growth at 37 degrees c, y. pestis lipopolysaccharide (lps) contained the tetra-acylated lipid iv(a) and smaller amounts of lipid iv(a) modified with c10 or c12 acyl groups, y. pseudotuberculosis c ... | 2004 | 15165239 |
| invasin and beyond: regulation of yersinia virulence by rova. | rova, a member of the marr/slya family of winged-helix transcription factors, regulates expression of invasin, the major adhesion and invasion factor in yersinia enterocolitica and yersinia pseudotuberculosis. disruption of rova increases the ld(50) of the organism when inoculated using the oral route. however, when administered by intraperitoneal injection only a slight difference in ld(50) between mutant and wild-type organisms is apparent. the study of rova and the genes it regulates provides ... | 2004 | 15165608 |
| a process for controlling intracellular bacterial infections induced by membrane injury. | strategies for inhibiting phagolysosome fusion are essential for the intracellular survival and replication of many pathogens. we found that the lysosomal synaptotagmin syt vii is required for a mechanism that promotes phagolysosomal fusion and limits the intracellular growth of pathogenic bacteria. syt vii was required for a form of ca2+-dependent phagolysosome fusion that is analogous to ca2+-regulated exocytosis of lysosomes, which can be triggered by membrane injury. bacterial type iii secre ... | 2004 | 15178804 |
| multiple outbreaks of yersinia pseudotuberculosis infections in finland. | during 2001, 89 culture-confirmed cases of yersinia pseudotuberculosis were reported in finland; 55 (62%) were serotype o:1, and 34 (38%) were serotype o:3. four major pulsed-field gel electrophoresis profiles were identified. a case-control study of 25 case patients and 71 healthy controls identified eating outside the home as a risk factor for infection. | 2004 | 15184472 |
| recombinant escherichia coli as a gene delivery vector into airway epithelial cells. | to transfer genes into airway epithelial cells, we have generated auxotrophic dap escherichia coli bm2710 mutant that expresses the invasin of yersinia pseudotuberculosis and the listeriolysin of listeria monocytogenes. e. coli bm2710 harboring a plasmid carrying the gfp gene was incubated with immortalized normal or cystic fibrosis (cf) airway epithelial cells or with primary bronchial epithelial cells grown as an explant-outgrowth cell culture model. approximately 2% of immortalized cells expr ... | 2004 | 15196763 |
| the yopd translocator of yersinia pseudotuberculosis is a multifunctional protein comprised of discrete domains. | to establish an infection, yersinia pseudotuberculosis utilizes a plasmid-encoded type iii translocon to microinject several anti-host yop effectors into the cytosol of target eukaryotic cells. yopd has been implicated in several key steps during yop effector translocation, including maintenance of yop regulatory control and pore formation in the target cell membrane through which effectors traverse. these functions are mediated, in part, by an interaction with the cognate chaperone, lcrh. to ga ... | 2004 | 15205412 |
| novel protein-protein interactions of the yersinia pestis type iii secretion system elucidated with a matrix analysis by surface plasmon resonance and mass spectrometry. | binary complexes formed by components of the yersinia pestis type iii secretion system were investigated by surface plasmon resonance (spr) and matrix-assisted laser desorption time-of-flight mass spectrometry. pairwise interactions between 15 recombinant yersinia outer proteins (yops), regulators, and chaperones were first identified by spr. mass spectrometry confirmed over 80% of the protein-protein interactions suggested by spr, and new binding partners were further characterized. the yop sec ... | 2004 | 15213222 |
| proteomic characterization of host response to yersinia pestis and near neighbors. | host-pathogen interactions result in protein expression changes within both the host and the pathogen. here, results from proteomic characterization of host response following exposure to yersinia pestis, the causative agent of plague, and to two near neighbors, yersinia pseudotuberculosis and yersinia enterocolitica, are reported. human monocyte-like cells were chosen as a model for macrophage immune response to pathogen exposure. two-dimensional electrophoresis followed by mass spectrometry wa ... | 2004 | 15219853 |
| characterization of outer membrane proteins of yersinia pestis and yersinia pseudotuberculosis strains isolated from india. | the majority of virulence factors including the 12 yersinia outer membrane proteins (yops), 29 yop secretion proteins (ysc) and few specific yop chaperone (syc) are contributed by the 70 kb lcr middle plasmid of yersinia pestis. yersinia pestis isolates recovered during 1994 plague outbreak and rodent surveillance samples of southern states of india were studied for the presence of important yops by the conventional procedure of partially purifying outer membrane proteins (omps) after cultivatio ... | 2004 | 15233478 |
| influence of yersinia pseudotuberculosis outer proteins (yops) on interleukin-12, tumor necrosis factor alpha and nitric oxide production by peritoneal macrophages. | an essential key to pathogenicity in yersinia is the presence of a 70 kb plasmid (pyv) which encodes a type-iii secretion system and several virulence outer proteins whose main function is to enable the bacteria to survive in the host. thus, a specific immune response is needed in which cytokines are engaged. the aim of this study was to assess the influence of yersinia outer proteins (yops) released by yersinia pseudotuberculosis on the production of the proinflammatory cytokines, interleukin-1 ... | 2004 | 15234540 |
| rova is autoregulated and antagonizes h-ns-mediated silencing of invasin and rova expression in yersinia pseudotuberculosis. | the transcriptional activator rova of yersinia pseudotuberculosis, a member of the slya/hor family, activates its own expression and that of the virulence factor invasin in response to moderate growth temperature, but not at 37 degrees c. in this work, we analysed the mechanism of rova-dependent transcription of the rova and inv genes. we found that rova is transcribed by two different promoters. sequences located upstream and downstream of the promoters were involved in rova autoregulation and ... | 2004 | 15255899 |
| a movable surface: formation of yersinia sp. biofilms on motile caenorhabditis elegans. | bubonic plague is transmitted by fleas whose feeding is blocked by a mass of yersinia pestis in the digestive tract. y. pestis and the closely related y. pseudotuberculosis also block the feeding of caenorhabditis elegans by forming a biofilm on the nematode head. c. elegans mutants with severe motility defects acquire almost no biofilm, indicating that normal animals accumulate the biofilm matrix as they move through a yersinia lawn. using the lectin wheat germ agglutinin as a probe, we show th ... | 2004 | 15262945 |
| dna microarray analysis of genome dynamics in yersinia pestis: insights into bacterial genome microevolution and niche adaptation. | genomics research provides an unprecedented opportunity for us to probe into the pathogenicity and evolution of the world's most deadly pathogenic bacterium, yersinia pestis, in minute detail. in our present work, extensive microarray analysis in conjunction with pcr validation revealed that there are considerable genome dynamics, due to gene acquisition and loss, in natural populations of y. pestis. we established a genomotyping system to group homologous isolates of y. pestis, based on profili ... | 2004 | 15262950 |
| yapi, a new yersinia pseudotuberculosis pathogenicity island. | pathogenicity islands (pais) are chromosomal clusters of pathogen-specific virulence genes often found at trna loci. in the yersinia pseudotuberculosis 32777 chromosome, we characterized a 98-kb segment that has all of the characteristic features of a pai, including insertion in a (phenylalanine) trna gene, the presence of a bacteriophage-like integrase-encoding gene, and direct repeats at the integration sites. the g+c content of the segment ranges from 31 to 60%, reflecting a genetic mosaic: t ... | 2004 | 15271940 |
| [prevalence of anti-yersinia antibodies in european brown hares in north-rhine westphalia, germany]. | yersinia (y.) pseudotuberculosis infections may lead to significant lethality in european brown hare (lepus europaeus, pallas) populations especially during the cold and wet seasons. in recent decades, also y. enterocolitica was isolated from hares found dead. consequently, a western-blot technique proved to be valuable for the detection of antibodies against all pathogenic yersinia isolates was applied to monitor the prevalence of antibodies in hare populations in north-rhine westphalia, german ... | 2004 | 15287580 |
| short communication: yersinia pseudotuberculosis septicemia in an hiv-infected patient failed haart. | the first case of septicemia due to yersinia pseudotuberculosis in an hiv-infected person was reported. the 42-year-old woman was severely immunosuppressed despite a prolonged exposure to haart. specific amplicons for inv, yada, and lcrf genes showed the pathogenetic potential of the y. pseudotuberculosis serotype o1 isolate. a favorable clinical response to ceftriaxone and levofloxacin was observed. | 2004 | 15307915 |
| the response regulator phop of yersinia pseudotuberculosis is important for replication in macrophages and for virulence. | yersinia pestis and yersinia pseudotuberculosis are closely related facultative intracellular pathogens. the response regulator phop was previously shown to be important for y. pestis survival in macrophages and for virulence in a murine bubonic plague infection assay. here the importance of phop for y. pseudotuberculosis pathogenesis was investigated. y. pseudotuberculosis phop mutants were unable to replicate in low-mg(2+) medium or in macrophages. phop(+) y. pseudotuberculosis strains initiat ... | 2004 | 15321989 |
| [characterization of yersinia pseudotuberculosis heat stable serovar specific polypeptides with the use of monoclonal antibodies]. | the capacity of y. pseudotuberculosis to express serovar specific polypeptides with different specificity of antigenic determinants was proved with the use of monoclonal antibodies (mcab). for the first time y. pseudotuberculosis o antigens were found to have heat stable protein components carrying linear epitopes complementary to serovar specific maab and ensuring the serological specificity of the infective agent. the possibility of improving intraspecific classification of y. pseudotuberculos ... | 2004 | 15346942 |
| [the action of yersinia pseudotuberculosis heat stable lethal toxin on the biosynthesis of protein in vitro]. | the results of the in vitro action of y. pseudotuberculosis heat stable lethal toxin on the biosynthesis of protein are presented. the toxin was shown to inhibit the inclusion of exogenous amino acid into newly synthesized peptides. the degree of the inhibition of the biosynthesis of protein in the in vitro system depends on the amount of the toxin added to the incubation mixture. the use of the conjugated transcription and translation system confirms our earlier data on the influence of the let ... | 2004 | 15346943 |
| [influence of volatile metabolites of saprophytic soil microflora on the propagation of pathogenic bacteria]. | the influence of volatile metabolites of saprophytic soil microflora on the propagation of listeria monocytogenes and yersinia pseudotuberculosis is shown. different character of interspecific relationships between bacteria, influencing their propagation, can be observed on the metabolic level. volatile compounds produced by microorganisms are capable to act as both intra- and interspecific regulators of microbial communities. in this connection the propagation of pathogenic bacteria inhabiting ... | 2004 | 15346944 |
| [physicochemical and immune properties of glycoglycerolipids from laminaria japonica within immunostimulating complexes (iscoms)]. | certain physicochemical properties of glycoglycerolipids from marine alga laminaria japonica (monogalactosyl diacylglycerol, digalactosyl diacylglycerol, and sulfoquinovosyl diacylglycerol) and their ability to be incorporated into immunostimulating complexes (iscoms) used for presentation of microbial and tumor antigens in vesicular form were comparatively described. these glycolipids proved to considerably differ by fatty acid composition, degree of unsaturation, and phase transition temperatu ... | 2004 | 15354955 |
| insights into the evolution of yersinia pestis through whole-genome comparison with yersinia pseudotuberculosis. | yersinia pestis, the causative agent of plague, is a highly uniform clone that diverged recently from the enteric pathogen yersinia pseudotuberculosis. despite their close genetic relationship, they differ radically in their pathogenicity and transmission. here, we report the complete genomic sequence of y. pseudotuberculosis ip32953 and its use for detailed genome comparisons with available y. pestis sequences. analyses of identified differences across a panel of yersinia isolates from around t ... | 2004 | 15358858 |
| toll-like receptor 2-dependent bacterial sensing does not occur via peptidoglycan recognition. | toll-like receptor 2 (tlr2) has been shown to recognize several classes of pathogen-associated molecular patterns including peptidoglycan (pg). however, studies linking pg with tlr2 recognition have relied mainly on the use of commercial staphylococcus aureus pg and have not addressed tlr2 recognition of other pg types. using highly purified pgs from eight bacteria (escherichia coli, pseudomonas aeruginosa, yersinia pseudotuberculosis, helicobacter pylori, bacillus subtilis, listeria monocytogen ... | 2004 | 15359270 |
| srf-3, a mutant of caenorhabditis elegans, resistant to bacterial infection and to biofilm binding, is deficient in glycoconjugates. | srf-3 is a mutant of c. elegans that is resistant to infection by microbacterium nematophilum and to binding of the biofilm produced by yersinia pseudotuberculosis and yersinia pestis. recently, srf-3 was characterized as a nucleotide sugar transporter of the golgi apparatus occurring exclusively in hypodermal seam cells, pharyngeal cells, and spermatheca. based on the above observations, we hypothesized that srf-3 may have altered glyconjugates that may enable the mutant nematode to grow unaffe ... | 2004 | 15452127 |
| modification of biological activity of lipopolysaccharide in the complex with chitosan. | in the complex with chitosan, lipopolysaccharide partially lost its ability to induce lymphokines tumor necrosis factor and interleukin-8, but retained immunostimulating properties and increased phagocytic function of macrophages by improving digestion of bacteria. | 2004 | 15452608 |
| repeated cycles of rapid actin assembly and disassembly on epithelial cell phagosomes. | we have found that early in infection of the intracellular pathogen listeria monocytogenes in madin-darby canine kidney epithelial cells expressing actin conjugated to green fluorescent protein, f-actin rapidly assembles (approximately 25 s) and disassembles (approximately 30 s) around the bacteria, a phenomenon we call flashing. l. monocytogenes strains unable to perform actin-based motility or unable to escape the phagosome were capable of flashing, suggesting that the actin assembly occurs on ... | 2004 | 15456901 |
| [variability of the clonal structure of yersinia pseudotuberculosis population under different conditions]. | in a series of experiments the dynamics of the clonal structure of y. pseudotuberculosis population was evaluated by cytopathogenicity in soil extract, as well as in associations with blue-green algae (cyanobacteria) and infusoria, under different temperature conditions. in all variants of experiments made at low environmental temperature (10 degrees c) a considerable part of y. pseudotuberculosis clones (25-40%) was found to be cytopathogenic, while at 22 degrees c such clones were absent or ha ... | 2004 | 15481916 |
| the eyam plague revisited: did the village isolation change transmission from fleas to pulmonary? | back in the 17th century the derbyshire village of eyam fell victim to the black death, which is thought to have arrived from london in some old clothes brought by a travelling tailor. the village population was 350 at the commencement of plague, of which only 83 survived. led by the church leaders, the village community realized that the whole surrounding region was at risk from the epidemic, and therefore decided to seal themselves off from the other surrounding villages. in the first 275 days ... | 2004 | 15488668 |
| comparative and evolutionary genomics of yersinia pestis. | yersinia pestis is the causative agent of plague, causing three human plague pandemics in history. comparative and evolutionary genomics of y. pestis are extensively discussed in this review. understanding the genomic variability and the adaptive evolution of y. pestis from the genomic point of view will contribute greatly to plague detection, identification, control and prevention. | 2004 | 15488743 |
| darwin's principle of divergence revisited: small steps and quantum leaps set the path of microbial evolution. | 2004 | 15493815 | |
| lcrv synthesis is altered by dna adenine methylase overproduction in yersinia pseudotuberculosis and is required to confer immunity in vaccinated hosts. | yersinia pseudotuberculosis mutants that overproduce the dna adenine methylase (damop yersinia) are attenuated, confer robust protective immune responses, and synthesize or secrete several yersinia outer proteins (yops) under conditions that are nonpermissive for synthesis and secretion in wild-type strains. to understand the molecular basis of immunity elicited by damop yersinia, we investigated the effects of dam overproduction on the synthesis and localization of a principal yersinia immunoge ... | 2004 | 15501808 |
| isolated gastric malakoplakia: a case report and review of the literature. | a 62-year-old african american woman presented with weight loss and dyspepsia. she did not have any clinical evidence of immunodeficiency. upper gastrointestinal endoscopy revealed multiple small polypoid lesions in the gastric body and fundus that appeared larger and more erythematous than usual fundic gland polyps. examination of biopsy specimens revealed an infiltrate of large histiocytes with eosinophilic granular cytoplasm located in the lamina propria and containing michaelis-gutmann bodie ... | 2004 | 15504079 |
| modulation of yersinia type three secretion system by the s1 domain of polynucleotide phosphorylase. | both low temperatures and encounters with host phagocytes are two stresses that have been relatively well studied in many species of bacteria. previous work has shown that the exoribonuclease polynucleotide phosphorylase (pnpase) is required for yersiniae to grow at low temperatures. here, we show that pnpase also enhances the ability of yersinia pseudotuberculosis and yersinia pestis to withstand the killing activities of murine macrophages. pnpase is required for the optimal functioning of the ... | 2005 | 15509583 |
| identification of an unusual [2fe-2s]-binding motif in the cdp-6-deoxy-d-glycero-l-threo-4-hexulose-3-dehydrase from yersinia pseudotuberculosis: implication for c-3 deoxygenation in the biosynthesis of 3,6-dideoxyhexoses. | cdp-6-deoxy-l-threo-d-glycero-4-hexulose-3-dehydrase (e(1)) catalyzes the c-3 deoxygenation in the biosynthesis of 3,6-dideoxyhexoses in yersinia pseudotuberculosis. e(1) is a pyridoxamine 5'-phosphate (pmp)-dependent enzyme that also contains a [2fe-2s] center. this iron-sulfur cluster is catalytically essential, since removal of the [2fe-2s] center leads to inactive enzyme. to identify the [2fe-2s] core in e(1) and to study the effect of impairing the iron-sulfur cluster on the activity of e(1 ... | 2004 | 15518577 |
| genome update: dna repeats in bacterial genomes. | 2004 | 15528640 | |
| sensitive and specific detection of yersinia pseudotuberculosis by loop-mediated isothermal amplification. | we developed a loop-mediated isothermal amplification method able to detect yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. this method is more sensitive than pcr and might be a useful tool for detecting and identifying y. pseudotuberculosis. | 2004 | 15528740 |
| "one size fits it all": translocation of foreign antigens by yersinia type iii secretion system (ttss) leads to concomitant cd4 and cd8 t-cell priming. | live replicating bacteria expressing heterologous antigens are vaccine candidates that are able to induce complex immune responses. yersinia pseudotuberculosis employs a type iii secretion system for translocation of several virulence factors directly to the cytosol of eukaryotic cells. mice orally inoculated with an attenuated recombinant yersinia strain translocating a chimeric yersinia outer protein e (yope) molecule reveal high numbers of foreign antigen-specific cd4 and cd8 t cells. thus, c ... | 2004 | 15532990 |
| evaluation of the role of constitutive isocitrate lyase activity in yersinia pestis infection of the flea vector and mammalian host. | yersinia pestis, unlike the closely related yersinia pseudotuberculosis, constitutively produces isocitrate lyase (icl). here we show that the y. pestis acea homologue encodes icl and is required for growth on acetate but not for flea infection or virulence in mice. thus, deregulation of the glyoxylate pathway does not underlie the recent adaptation of y. pestis to arthropod-borne transmission. | 2004 | 15557663 |
| experimental kinetics of infection induced by yersinia pseudotuberculosis isolated from stock animals. | the course of in vivo infection of five isolates of yersinia pseudotuberculosis was followed for three weeks in swiss mice. the strains were isolated from diarrheic and normal feces and mesenteric lymph nodes of healthy and sick stock animals. four strains of serogroup o:3 and one of serogroup o:1a, with and without the virulence plasmid, were inoculated intragastrically and intravenously in the mice. groups of five animals were sacrificed at 6 h and 3, 6, 10, 15, and 21 days after inoculation, ... | 2004 | 15558174 |
| acute toxoplasmosis in three wild arctic foxes (alopex lagopus) from svalbard; one with co-infections of salmonella enteritidis pt1 and yersinia pseudotuberculosis serotype 2b. | acute disseminated toxoplasmosis was diagnosed in three wild arctic foxes (alopex lagopus) that were found dead in the same locality on svalbard (norway). the animals included one adult female and two 4-months-old pups. the adult fox was severely jaundiced. necropsy revealed multifocal, acute, necrotizing hepatitis, acute interstitial pneumonia, and scattered foci of brain gliosis, often associated with toxoplasma tachyzoites. one pup also had toxoplasma-associated meningitis. in addition, the l ... | 2005 | 15563924 |
| granulomatous appendicitis. | granulomatous inflammation of the appendix is uncommon. it can be caused by a variety of conditions, including systemic disorders such as crohn's disease and sarcoidosis, and infections such as mycobacterium tuberculosis, yersinia pseudotuberculosis, parasites and fungi. granulomatous appendicitis as an isolated pathological entity unassociated with systemic disease is rare. isolated granulomatous inflammation of the appendix of unknown aetiology, otherwise known as idiopathic granulomatous appe ... | 2003 | 15570781 |
| the pmrf polymyxin-resistance operon of yersinia pseudotuberculosis is upregulated by the phop-phoq two-component system but not by pmra-pmrb, and is not required for virulence. | the yersinia pseudotuberculosis chromosome contains a seven-gene polycistronic unit (the pmrf operon) whose products share extensive homologies with their pmrf counterparts in salmonella enterica serovar typhimurium (s. typhimurium), another gram-negative bacterial enteropathogen. this gene cluster is essential for addition of 4-aminoarabinose to the lipid moiety of lps, as demonstrated by maldi-tof mass spectrometry of lipid a from both wild-type and pmrf-mutated strains. as in s. typhimurium, ... | 2004 | 15583148 |
| microevolution and history of the plague bacillus, yersinia pestis. | the association of historical plague pandemics with yersinia pestis remains controversial, partly because the evolutionary history of this largely monomorphic bacterium was unknown. the microevolution of y. pestis was therefore investigated by three different multilocus molecular methods, targeting genomewide synonymous snps, variation in number of tandem repeats, and insertion of is100 insertion elements. eight populations were recognized by the three methods, and we propose an evolutionary tre ... | 2004 | 15598742 |
| biosynthesis of colitose: expression, purification, and mechanistic characterization of gdp-4-keto-6-deoxy-d-mannose-3-dehydrase (cold) and gdp-l-colitose synthase (colc). | l-colitose is a 3,6-dideoxyhexose found in the o-antigen of gram-negative lipopolysaccharides. to study the biosynthesis of this unusual sugar, we have cloned and sequenced the l-colitose biosynthetic gene cluster from yersinia pseudotuberculosis vi. the cold and colc genes in this cluster have been overexpressed and each gene product has been purified and characterized. our results showed that cold functions as gdp-4-keto-6-deoxy-d-mannose-3-dehydrase responsible for c-3 deoxygenation of gdp-4- ... | 2004 | 15610039 |
| cell invasion and il-8 production pathways initiated by yada of yersinia pseudotuberculosis require common signalling molecules (fak, c-src, ras) and distinct cell factors. | the yada protein of yersinia pseudotuberculosis promotes tight adhesion and invasion into mammalian cells through beta(1)-integrins. in this work, we demonstrate that yada also triggers the production of interleukin-8 (il-8) in host cells and we identify intracellular signal transduction mechanisms involved in yada-initiated cell invasion and/or il-8 synthesis. tyrosine protein kinases, including the focal adhesion kinase (fak) and c-src, as well as the small gtpase ras, were shown to play a sig ... | 2005 | 15617524 |
| [method for the calculation of the 50% effective dose of biologically active agents]. | a newly proposed method for the mathematical and graphic determination and calculation of ed50 (ld50) on the abscissa at the meeting point of the cumulate of dead and the cumulate of survived animals (in absolute figures) in "dose-effect) experiments is described. "the method of meeting cumulates) for the calculation of ed50 (ld50) is simple, eliminates unnecessary calculations, yields results, highly similar (95-100%) to those obtained by other methods and may be used in different medico-biolog ... | 2004 | 15636133 |