Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| anti-immunoglobulin and phorbol ester induce phosphorylation of proteins associated with the plasma membrane and cytoskeleton in murine b lymphocytes. | protein phosphorylations are rapidly induced in intact b cells by antibodies to surface immunoglobulin (anti-igm) and by phorbol 12-myristate 13-acetate (pma). a comparison of the molecular weight, isoelectric points, phosphopeptides, and phosphoamino acids of the phosphoproteins induced by anti-igm and by pma suggests that anti-igm acts through the activation of protein kinase c. this conclusion is strengthened by the observation that prolonged treatment with pma ablates the ability of anti-igm ... | 2010 | 3021753 |
| anti-immunoglobulin and phorbol ester induce phosphorylation of proteins associated with the plasma membrane and cytoskeleton in murine b lymphocytes. | protein phosphorylations are rapidly induced in intact b cells by antibodies to surface immunoglobulin (anti-igm) and by phorbol 12-myristate 13-acetate (pma). a comparison of the molecular weight, isoelectric points, phosphopeptides, and phosphoamino acids of the phosphoproteins induced by anti-igm and by pma suggests that anti-igm acts through the activation of protein kinase c. this conclusion is strengthened by the observation that prolonged treatment with pma ablates the ability of anti-igm ... | 2010 | 3021753 |
| human leukocyte antigens and sister chromatid exchanges in families with multiple adenomatosis coli. | to study human leukocyte antigens (hla) and sister chromatid exchanges (sce) in multiple adenomatosis coli (mac), eight families were evaluated. twenty-five subjects (14 affected members and 11 healthy relatives) were studied with hla a, b, and c typing of peripheral lymphocytes and sce count in cells that had replicated twice. the results of the present immunogenetic study, although limited to a small number of cases, suggest a correlation between hla, sce, and mac. when the results of further ... | 1986 | 3022927 |
| partial transformation of mouse fibroblastic and epithelial cell lines with the v-myc oncogene. | to investigate the role of the myc gene in mammalian cell transformation, plasmid constructs containing the v-myc oncogene and a co-selectable g418 resistance marker were introduced into both mouse fibroblasts (nih-3t3) and bladder epithelial cells (bbn3 and bbn7). after transfection or microinjection of dna, no transformed foci could be detected on confluent monolayers but, when the cells were cultured under conditions in which individual cells were allowed to grow and form colonies, morphologi ... | 1987 | 3023119 |
| total iridectomy does not alter outflow facility responses to cyclic amp in cynomolgus monkeys. | total outflow facility was measured by two-level constant pressure anterior chamber perfusion in surgically untouched and totally iridectomized cynomolgus monkey eyes receiving bolus intracameral infusions of cyclic amp, dibutyryl cyclic amp, or 5'amp. cyclic amp doses of 50 and 100 micrograms (corresponding to initial intracameral concentrations of 1.5- and 3 mm) increased facility by approximately 20 and 40% respectively in both surgically untouched and aniridic eyes. higher doses produced no ... | 1986 | 3023122 |
| a conserved human germline v kappa gene directly encodes rheumatoid factor light chains. | the full-length gene that encodes the light chain variable regions of an idiotypically related group of human igm kappa rheumatoid factors (rfs) has been cloned and sequenced. the deduced amino acid sequence is identical to four separate rf proteins. these results prove that genes capable of encoding human anti-igg autoantibody light chains without any somatic mutation are present in the kappa gene repertoire of normal people. | 1986 | 3023521 |
| a conserved human germline v kappa gene directly encodes rheumatoid factor light chains. | the full-length gene that encodes the light chain variable regions of an idiotypically related group of human igm kappa rheumatoid factors (rfs) has been cloned and sequenced. the deduced amino acid sequence is identical to four separate rf proteins. these results prove that genes capable of encoding human anti-igg autoantibody light chains without any somatic mutation are present in the kappa gene repertoire of normal people. | 1986 | 3023521 |
| initiation of swimming activity by trigger neurons in the leech subesophageal ganglion. iii. sensory inputs to tr1 and tr2. | in papers i and ii of this series, we described two pairs of interneurons, tr1 and tr2, in the leech subesophageal ganglion which can trigger swimming activity in the isolated central nervous system (cns). in this paper, we describe sensory inputs to these trigger neurons from previously identified mechanosensory neurons. we found that: weak mechanical stimulation (stroking) of a body wall flap attached to a segmental ganglion in an otherwise isolated cns excites the contralateral tr1 slightly. ... | 2010 | 3023604 |
| mutations linked to the pro alpha 2(i) collagen gene are responsible for several cases of osteogenesis imperfecta type i. | we have analysed six south african families with osteogenesis imperfecta type i using three dna polymorphisms associated with the pro alpha 2(i) collagen gene. in four of these families linkage of the pro alpha 2(i) gene and the osteogenesis imperfecta phenotype was suggested, whereas in the remaining two families there was a lack of linkage. no distinct correlation could be made between the phenotypic features of the families studied and linkage or lack of linkage to the pro alpha 2(i) gene. tw ... | 2010 | 3023615 |
| structure of replicating simian virus 40 minichromosomes. the replication fork, core histone segregation and terminal structures. | the structure of replicating simian virus 40 (sv40) minichromosomes was studied by dna crosslinking with trimethyl-psoralen. the procedure was used both in vitro with extracted sv40 minichromosomes as well as in vivo with sv40-infected cells. both procedures gave essentially the same results. mature sv40 minichromosomes are estimated to contain about 27 nucleosomes (error +/- 2), except for those molecules with a nucleosome-free gap, which are interpreted to contain 25 nucleosomes (error +/- 2). ... | 1986 | 3023620 |
| relative activities of 5'-nucleotidase and adenosine deaminase in atrial and ventricular myocardium--the enzyme paradox. | the 5'-nucleotidase and adenosine deaminase activities were determined for tissue extracts of up to 10 defined regions of the normal dog heart. the specific activity of 5'-nucleotidase was very significantly higher in atrial than in ventricular myocardium (4 x) and was also higher in right than in left sites of the heart (2 x). the reason for the markedly higher specific activity in atrial muscle is not clear and appears paradoxical relative to adenine nucleotide content and pattern of atp decay ... | 1986 | 3025011 |
| epidemiology of borderline ovarian tumors. | ovarian tumors of low malignant potential, often termed "borderline tumors," have been defined as those that have some but not all of the morphologic features of malignancy (i.e., they are not invasive). with the use of data obtained by the western washington population-based cancer surveillance system for 1975-83, the incidence of serous and mucinous borderline epithelial ovarian tumors was analyzed, as well as the survival of women who developed them. the incidence of borderline tumors increas ... | 2010 | 3025504 |
| epidemiology of borderline ovarian tumors. | ovarian tumors of low malignant potential, often termed "borderline tumors," have been defined as those that have some but not all of the morphologic features of malignancy (i.e., they are not invasive). with the use of data obtained by the western washington population-based cancer surveillance system for 1975-83, the incidence of serous and mucinous borderline epithelial ovarian tumors was analyzed, as well as the survival of women who developed them. the incidence of borderline tumors increas ... | 2010 | 3025504 |
| structurally related bacillus thuringiensis delta-endotoxins display major differences in insecticidal activity in vivo and in vitro. | many strains within the 22 serotypes of bacillus thuringiensis produce crystal delta-endotoxins with slight differences in their insecticidal toxicity spectrum in vivo. since the basis of this specificity is unknown, we chose to compare the activity of delta-endotoxins from three strains: b. thuringiensis var. kurstaki hd-1, var. aizawai hd-249 and var. thuringiensis hd-350, both in vivo and on insect cell lines in vitro. immunoblotting with antisera to activated var. kurstaki p1 lepidopteran to ... | 1986 | 3027111 |
| nucleotide sequence of the virulence gene virg of the agrobacterium tumefaciens octopine ti plasmid: significant homology between virg and the regulatory genes ompr, phob and dye of e. coli. | the entire nucleotide sequence of the virg locus, from the octopine ti plasmid of agrobacterium tumefaciens strain 15955, has been determined. the virg gene is 801 nucleotides in length and has one open reading frame which encodes a protein of mr 29,995. the virg gene is involved in the transcriptional activation of the ti plasmid vir-loci, which occurs after induction by specific compounds present in plant exudate. sequence analysis of the agrobacterium virg protein showed significant homology ... | 1986 | 3027669 |
| the effects of electroconvulsive therapy and antidepressant drugs on monoamine receptors in rodent brain--similarities and differences. | repeated administration to rodents of electroconvulsive shock (ecs) produces changes in brain monoamine biochemistry and function, several of which are also seen after repeated administration of antidepressant drugs. both repeated ecs and antidepressant drug administration decrease cortical beta-adrenoceptor density and attenuate the alpha 2-adrenoceptor-mediated sedation response to clonidine injection. neither procedure alters phenylephrine-induced locomotor activity in mice, a measure of alph ... | 1986 | 3028726 |
| the effects of electroconvulsive therapy and antidepressant drugs on monoamine receptors in rodent brain--similarities and differences. | repeated administration to rodents of electroconvulsive shock (ecs) produces changes in brain monoamine biochemistry and function, several of which are also seen after repeated administration of antidepressant drugs. both repeated ecs and antidepressant drug administration decrease cortical beta-adrenoceptor density and attenuate the alpha 2-adrenoceptor-mediated sedation response to clonidine injection. neither procedure alters phenylephrine-induced locomotor activity in mice, a measure of alph ... | 1986 | 3028726 |
| reconstitution of vesicle fusions occurring in endocytosis with a cell-free system. | we have used defined subcellular fractions to reconstitute in a cell-free system vesicle fusions occurring in the endocytic pathway. the endosomal fractions were prepared by immuno-isolation using as antigen an epitope located on a foreign protein, the transmembrane glycoprotein g (g-protein) of vesicular stomatitis virus. the g-protein was first implanted in the cell plasma membrane and subsequently endocytosed for 15 to 30 min at 37 degrees c. the endosomal fractions were immuno-isolated on a ... | 1986 | 3028771 |
| reconstitution of vesicle fusions occurring in endocytosis with a cell-free system. | we have used defined subcellular fractions to reconstitute in a cell-free system vesicle fusions occurring in the endocytic pathway. the endosomal fractions were prepared by immuno-isolation using as antigen an epitope located on a foreign protein, the transmembrane glycoprotein g (g-protein) of vesicular stomatitis virus. the g-protein was first implanted in the cell plasma membrane and subsequently endocytosed for 15 to 30 min at 37 degrees c. the endosomal fractions were immuno-isolated on a ... | 1986 | 3028771 |
| phospholipase c contracts visceral smooth muscle. | phospholipase c added into the muscle bath (0.2-1.5 units/ml) contracted the guinea-pig taenia coli in a concentration-dependent manner. the fully developed contraction appeared within second, like the contractile effect evoked by 5 microm acetylcholine. pharmacochemical dissection (using phospholipase d, indomethacin, neomycin, li+, phorbol ester and oleoyl-acetyl-glycerol) showed that the contractions induced by exogenous phospholipase c are mediated by inositol trisphosphate, not by diacylgly ... | 1986 | 3028825 |
| phospholipase c contracts visceral smooth muscle. | phospholipase c added into the muscle bath (0.2-1.5 units/ml) contracted the guinea-pig taenia coli in a concentration-dependent manner. the fully developed contraction appeared within second, like the contractile effect evoked by 5 microm acetylcholine. pharmacochemical dissection (using phospholipase d, indomethacin, neomycin, li+, phorbol ester and oleoyl-acetyl-glycerol) showed that the contractions induced by exogenous phospholipase c are mediated by inositol trisphosphate, not by diacylgly ... | 1986 | 3028825 |
| virulence of iron transport mutants of shigella flexneri and utilization of host iron compounds. | mutants of shigella flexneri defective in aerobactin-mediated iron transport were assayed for virulence in several model systems. a tn5 insertion mutant was invasive in hela cells, lethal in the chicken embryo, and produced keratoconjunctivitis in the guinea pig, indicating little or no loss of ability to invade and multiply intracellularly. although the mutant failed to grow in low-iron medium in vitro, growth equivalent to that of the wild type was observed in hela cell lysates. thus, there ap ... | 1987 | 3028962 |
| test anxiety in master's students: a comparative study. | evaluation events are major problems for graduate students in nursing. the purpose of this study was to determine the correlation of test anxiety before and cognitive interferences during a comprehensive examination. the sample (n = 54) consisted of two groups of students (group i, n = 30 and group ii, n = 24) in a master's program at a state university. data were collected using three questionnaires: to measure general test anxiety, the test anxiety questionnaire (taq) by sarason was used; the ... | 2011 | 3029346 |
| porous hydroxyapatite as a bone graft substitute in diaphyseal defects: a histometric study. | porous hydroxyapatite (ip200), formed by conversion of the poritidae porites exoskeleton, has pores averaging 230 microns and pore interconnections averaging 190 microns in diameter. in the distal radial diaphyses of 14 dogs, bilateral 7.5 x 20 mm cortical windows were created and fitted with 5 x 7.5 x 20 mm blocks of ip200 implants and iliac autografts. both implanted and contralateral grafted radius specimens were retrieved at 3, 6, 12, 24, and 48 months. unstained undecalcified sections were ... | 1987 | 3029358 |
| on the fidelity of dna replication: herpes dna polymerase and its associated exonuclease. | procaryotic dna polymerases contain an associated 3'----5' exonuclease activity which provides a proofreading function and contributes substantially to replication fidelity. dna polymerases of the eucaryotic herpes-type viruses contain similar associated exonuclease activities. we have investigated the fidelity of polymerases purified from wild type herpes simplex virus, as well as from mutator and antimutator strains. on synthetic templates, the herpes enzymes show greater relative exonuclease ... | 1987 | 3029700 |
| phospholipid metabolism and stimulus-response coupling. | 2010 | 3030324 | |
| phospholipid metabolism and stimulus-response coupling. | 2010 | 3030324 | |
| [effect of thymosin on afferent transmission in the somatosensory system of macaca rhesus]. | 2010 | 3030681 | |
| [effect of thymosin on afferent transmission in the somatosensory system of macaca rhesus]. | 2010 | 3030681 | |
| a cellular protein binds to a conserved sequence in the adenovirus type 2 enhancer. | a sensitive gel retention assay has been utilized to detect proteins from uninfected hela nuclei which interact with the adenovirus type 2 enhancer. this assay has been employed to monitor fractionation of nuclear extracts. three enhancer binding factors were resolved by chromatography on deae-sepharose and one of the factors was further purified by chromatography on heparin-sepharose. dnase protection experiments have shown that the heparin-sepharose fraction contains a factor which binds predo ... | 1987 | 3031610 |
| interactions between catecholamines and their receptors in blood. | 2010 | 3032424 | |
| integration and expression of provirus in human cells transformed by avian sarcoma virus. | previously, human diploid fibroblasts from some donors infected in vitro by avian sarcoma virus (asv) were transformed and found, by electron microscopy, to produce small numbers of virus particles that were infectious by bioassay; also, a line of human osteosarcoma cells infected with asv developed additional characteristics of transformation and released a small number of infectious virus particles. in this study the complete proviral sequence was shown to be integrated in the genome of these ... | 1987 | 3033382 |
| effects of inhibitors of the cytoplasmic structures and functions on the early phase of infection of cultured cells with simian virus 40. | to obtain information about cytoplasmic structures and functions involving the entry of simian virus 40 virions into cells, we examined whether the inhibitors that affect the functions and/or structure of lysosomes, cell membrane, and cytoskeletons inhibit expression of nuclear t antigen in the sv40-inoculated rat 3y1 and monkey cv-1 cells. chloroquine, methylamine, and butylamine did not inhibit t-antigen expression, suggesting that lysosomal acidification is not required for establishment of i ... | 1987 | 3033894 |
| the effect of cycloleucine on sfv a7(74) infection in mice. | cycloleucine (cl), a non-metabolizable amino acid analogue, was found to reduce thymus and spleen weights in semliki forest virus (sfv) strain a7(74) infected and control mice. the maximum effects were seen when three daily doses of cl were given to mice 24 h after an sfv a7(74) infection. in these mice thymus atrophy led to abolition of thymus dependent immune responses and changes in the pathological features of the viral infection--the most striking feature being prevention of demyelination. ... | 1987 | 3034321 |
| the student health center: a unique clinical experience for nursing leadership students. | 2010 | 3035135 | |
| chronic food restriction and the circadian rhythms of pituitary-adrenal hormones, growth hormone and thyroid-stimulating hormone. | adult male sprague-dawley rats were subjected to food restriction so that they ate 65% of food ingested by control rats. while control rats had free access to food over the 24-hour period, food-restricted rats were provided with food daily at 10 a.m. the experimental period lasted for 34 days. on day 35, rats from both experimental groups were killed at 08.00, 11.00, 14.00, 24.00 and 02.00 h. food restriction modified the circadian rhythms of acth and corticosterone. in addition, total circulati ... | 1987 | 3035995 |
| chronic food restriction and the circadian rhythms of pituitary-adrenal hormones, growth hormone and thyroid-stimulating hormone. | adult male sprague-dawley rats were subjected to food restriction so that they ate 65% of food ingested by control rats. while control rats had free access to food over the 24-hour period, food-restricted rats were provided with food daily at 10 a.m. the experimental period lasted for 34 days. on day 35, rats from both experimental groups were killed at 08.00, 11.00, 14.00, 24.00 and 02.00 h. food restriction modified the circadian rhythms of acth and corticosterone. in addition, total circulati ... | 1987 | 3035995 |
| [expressing plasmid vectors on the basis of escherichia coli beta-galactosidase gene fragments]. | with the use of synthetic dna fragments, a set of new plasmid vectors has been obtained. the vectors provided high level expression of peptides and small proteins in e. coli as fusions with fragments of beta-galactosidase of various length. these vectors were used to achieve expression of a synthetic gene for a functionally active fragment of bacteriorhodopsin. the yields of hybrid proteins consisting of beta-galactosidase and bacteriorhodopsin fragments were in the range of 5-30% from the total ... | 1987 | 3036168 |
| a high-yield, high-purity elutriation method for preparing human granulocytes demonstrating enhanced experimental lifetimes. | a counterflow centrifugal elutriation procedure for preparing human granulocytes is described. this method permits isolation of 1-2 x 10(9) granulocytes with a yield of 70% +/- 7% from a unit of whole blood in 3 h. these cells are 99% +/- 1% pure and 95-99% viable. when compared to cells prepared by a conventional procedure employing hypotonic lysis to remove red cells, the elutriated cells show enhanced oxidative responses resulting from enhanced longevity. other responses including receptor-me ... | 1987 | 3036986 |
| selective effects of ect on hypothalamic-pituitary activity. | the hypothesis that ect produces selective effects on hypothalamic-pituitary activity was investigated by determining the effect of ect on pituitary hormone release in nine depressed patients. after ect there were massive and rapid increases in the plasma concentrations of nicotine- and oestrogen-stimulated neurophysin (nsn and esn), prolactin (prl) and adrenocorticotropin (acth), smaller increases in plasma luteinizing hormone (lh) and cortisol, a significant decrease in plasma growth hormone ( ... | 2010 | 3037582 |
| structure of the 3' region of the human elastin gene: great abundance of alu repetitive sequences and few coding sequences. | two overlapping clones encompassing 8.5 kb of the human elastin gene were isolated from two genomic libraries constructed by partial digestion with either haeiii/alui or sau3a and contained in lambda charon 4a or embl3, respectively. the 6 kb of dna comprising the most 3' portion of the gene were sequenced demonstrating an extremely low coding ratio since only three exons containing a total of 134 translated nucleotides were identified. two exons totaling 78 bp of translated sequences which were ... | 1987 | 3038460 |
| structure of the 3' region of the human elastin gene: great abundance of alu repetitive sequences and few coding sequences. | two overlapping clones encompassing 8.5 kb of the human elastin gene were isolated from two genomic libraries constructed by partial digestion with either haeiii/alui or sau3a and contained in lambda charon 4a or embl3, respectively. the 6 kb of dna comprising the most 3' portion of the gene were sequenced demonstrating an extremely low coding ratio since only three exons containing a total of 134 translated nucleotides were identified. two exons totaling 78 bp of translated sequences which were ... | 1987 | 3038460 |
| complete amino acid sequence of cytochrome c551 from erythrobacter species strain och 114. | the complete amino acid sequence of cytochrome c551 isolated from an aerobic photosynthetic bacterium, erythrobacter sp. strain och 114, was determined. the cytochrome molecule was composed of a total of 119 amino acid residues and its molecular weight including heme was calculated to be 13,235. the sequence was (sequence: see text). its molecular weight indicates that this cytochrome is of the l-type. sequence alignment with other bacterial cytochromes c shows that this cytochrome is similar to ... | 1987 | 3038859 |
| [functional macrophage activity in infection with virulent and avirulent strains of the dysentery microbe]. | the effect of s. flexneri virulent and avirulent (vaccine) strains 2a on the cytoplasmic membrane of mouse macrophages has been studied by evaluating the action of these bacteria on the activity of 5-nucleotidase. the dynamics of the activity of 5-nucleotidase after the introduction of both virulent and avirulent strains has a phasic character with alternating rises and falls in the activity of this enzyme in comparison with the control. s. flexneri vaccine strain produces mainly a stimulating e ... | 1987 | 3039764 |
| investigation of adrenomedullary function in atopic dermatitis. | adrenomedullary function in patients with atopic dermatitis was assessed by measurement of plasma levels of catecholamines (adrenalin and noradrenalin) and cyclic amp in response to the stimuli of standing after lying supine, and a 5-min infusion of histamine in the standing position (i.e. histamine plus standing). plasma clearance of adrenalin was examined by measurement of plasma catecholamine and cyclic amp levels following a 15-min intravenous infusion of adrenalin in the supine position. re ... | 2011 | 3040069 |
| evaluation with an iuc::tn10 mutant of the role of aerobactin production in the virulence of shigella flexneri. | to evaluate the role of aerobactin production in the virulence of shigella flexneri, a iuc::tn10 insertion mutant was obtained from strain m90t, a serotype 5 isolate. this mutant was tested for its ability to invade and kill hela cells in monolayers, to elicit keratoconjunctivitis in guinea pigs and to infect ligated segments of rabbit ileal loops. although this mutant did not grow in iron-depleted media, its ability to grow intracellulalry and eventually kill hela cells was unchanged from that ... | 1987 | 3040587 |
| identification of glycoprotein ib beta as one of the major proteins phosphorylated during exposure of intact platelets to agents that activate cyclic amp-dependent protein kinase. | platelet function is inhibited by prostaglandin e1, prostaglandin i2, or forskolin, agents that increase the intracellular concentration of cyclic amp. the inhibition appears to result from cyclic amp-stimulated phosphorylation of specific intracellular proteins. one of the major increases in phosphorylation occurs in a polypeptide of mr = 24,000 (p24). in this study, an effort was made to identify p24. platelets prelabeled with [32p]phosphate were incubated with prostaglandin e1, prostaglandin ... | 2010 | 3040761 |
| [2 cases of creutzfeldt-jacob disease with bilateral vocal cord paralysis]. | 2009 | 3042238 | |
| [surgical therapy of prenatally detected abdominal abnormalities]. | 2010 | 3042547 | |
| [surgical therapy of prenatally detected abdominal abnormalities]. | 2010 | 3042547 | |
| biphasic radiation-sensitivity of the transplantation immunity for rejection of an allogeneic ascites tumor. | transplantation immunity for second-set rejection of an allogeneic ascites tumor was induced by sensitizing mice with h-2-identical allogeneic spleen cells, and radiation-sensitivity of this immunity was studied. the immunity was not severely affected by 400 rads whole-body x-ray irradiation given at one day before the initial antigenic stimulation. in contrast, it was totally inactivated by 300-400 rads irradiation that was given one day before tumor challenge. an adoptive cell transfer experim ... | 2011 | 3042613 |
| [ultrasonics and directional doppler. study of superficial phlebitis of the lower extremities]. | the objective of the study was to evaluate the usefulness of ultrasonic methods: ultrasonography and doppler c.w. in the diagnosis of superficial varicose phlebitis. especially, measurement of the venous pressure (v.p.) at the posterior tibial (p.t.) and the greater saphenous vein (g.s.v.) in clino and orthostatism, have proved to be useful. in ten cases, one patient with a varicose in the thigh, was also found to have a femoral vein thrombosis secondary to a deep thrombus entering the junction. ... | 2011 | 3043475 |
| the epidemiology of s. sonnei and s. flexneri in pima county, arizona: an exploratory study. | investigation of 189 cases of shigellosis reported to the pima county, arizona health department in 1986 revealed that 23 per cent of cases could be attributed to travel to mexico, and 10 per cent to day care attendees and their household contacts. no source of infection or high-risk activity could be demonstrated for 43 per cent of the cases. households in which s. flexneri occurred were more likely to be characterized by crowded living situations and to have no known source of infection. | 1988 | 3044149 |
| isolation of shiga toxin-resistant vero cells and their use for easy identification of the toxin. | shiga toxin-resistant vero cells were isolated by treatment of the cells with nitrosoguanidine. these mutant cells were not affected by shiga toxin at more than 1 microgram/ml, although the parent vero cells were sensitive to 25 pg of the toxin per ml. immunofluorescence studies showed that all the mutant cells had lost toxin-binding capacity. the cytotoxic activities of various bacterial cultures against the parent and mutant cells were compared. all samples from 10 strains of shigella dysenter ... | 1988 | 3045003 |
| congo red binding and salt aggregation as indicators of virulence in shigella species. | smooth strains of shigella dysenteriae type 1, shigella flexneri, shigella boydii, and shigella sonnei which form pigmented colonies (pcr+) on congo red agar were virulent in the sereny test. smooth variants unable to bind congo red (pcr-) were avirulent. measurements of dye uptake from solution showed that s. dysenteriae type 1 bound the most dye, followed in order of uptake by s. flexneri, s. boydii, and s. sonnei. using the salt aggregation test (sat) to determine cell surface hydrophobicity, ... | 1988 | 3045151 |
| clinical and microbiologic features of shigella and enteroinvasive escherichia coli infections detected by dna hybridization. | to determine the clinical and microbiologic features of shigella and enteroinvasive escherichia coli (eiec) infections, we investigated 410 children with diarrhea and 410 control children without diarrhea who were seen at children's hospital, bangkok, thailand, from january to june 1985. shigella spp. were isolated from 96 (23%) and eiec were isolated from 17 (4%) of 410 children with diarrhea and from 12 (3%) and 6 (1%) of 410 control children, respectively. the isolation rates of both pathogen ... | 1988 | 3045152 |
| glycocalyx in virulent and avirulent strains of shigella flexneri. | 1988 | 3045162 | |
| [morphologic manifestations of the medicinal lysis of a coronary artery thrombus]. | morphologic studies of coronary arterial thrombi have shown immature fibrin fibres to prevail in those, with fibrin degradation products always present. this fact suggests that fibrinolytic components of hemostasis, limiting the rates of fibrin polymerization and maturation, may participate in thrombogenesis. at the same time, macrophages that phagocytose fibrin and blood cells (platelets and erythrocytes) become activated since the early hours of the thrombus' existence. selective administratio ... | 1988 | 3045402 |
| [mechanism of the nonspecific protective action of shigella antigens]. | the mechanism promoting the nonspecific action of antigens obtained from s. flexneri and s. sonnei by a sparing method has been studied. these antigens stimulate the t- and b- systems of immunity, that is followed by activation of myelopoiesis and the humoral protective factors of the body, which seems to underlie the formation of resistance to infection caused by nonspecific microorganisms. | 1988 | 3046195 |
| measurement of l-[1-14c]leucine kinetics in splanchnic and leg tissues in humans. effect of amino acid infusion. | although whole-body leucine flux is widely measured to study body protein turnover in humans, the contribution of specific tissues to the total-body measurement remains unknown. by combining the organ-balance technique with the systemic infusion of l-[1-14c]leucine, we quantitated leucine production and disposal by splanchnic and leg tissues and by the whole body, simultaneously, in six normal men before and during amino acid infusion. at steady state, disposal of arterial leucine by splanchnic ... | 2011 | 3046968 |
| immunoreactivity of a surface wall fraction produced by spherules of coccidioides immitis. | the membranous spherule outer wall (sow) isolated from liquid cultures of coccidioides immitis has been shown to elicit reactivity with human anti-coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. the serologically reactive components were extracted from sow with the nonionic detergent n-octyl-beta-d-glucopyranoside (og). the og-soluble fraction of sow was shown to be reactive with immunoglobulin g in 25 serum samples from coccidioidomycosis patients by a ... | 1988 | 3047063 |
| immunoreactivity of a surface wall fraction produced by spherules of coccidioides immitis. | the membranous spherule outer wall (sow) isolated from liquid cultures of coccidioides immitis has been shown to elicit reactivity with human anti-coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. the serologically reactive components were extracted from sow with the nonionic detergent n-octyl-beta-d-glucopyranoside (og). the og-soluble fraction of sow was shown to be reactive with immunoglobulin g in 25 serum samples from coccidioidomycosis patients by a ... | 1988 | 3047063 |
| clinical trials of intramuscular sulprostone for second trimester abortion. | the synthetic prostaglandin e2 derivative sulprostone (16-phenoxy-w-17, 18, 19, 20-tetranor) is an effective 2nd trimester abortifacient. this study compares 2 dosage regimens of intramuscular administration. in regimen 1, 9 women with intact pregnancy and 3 with intrauterine fetal death were given sulprostone 500 mcg 3 times at 4-hour intervals to be repeated after 24 hours if abortion did not occur. in regimen 2, 12 women with intact pregnancies were given sulprostone 500 ug every 6 hours for ... | 2011 | 3047304 |
| [lipid peroxidation in the pathogenetic and clinical aspects of infectious diseases]. | 1988 | 3047989 | |
| blood-injury phobia: a review. | natural human uneasiness about blood, injury, or deformity sometimes becomes a specific phobia, which can lead to serious disability if vital medical procedures are refused. blood-injury phobia usually starts in childhood and is often familial. unlike other phobic cues, which cause persistent tachycardia, blood-injury phobic cues evoke an initial rise in heart rate followed by vasovagal bradycardia and, frequently, syncope. although blood-injury phobia may have an evolutionary, genetic, and phys ... | 1988 | 3048117 |
| [the extension of deep venous thrombosis treated by intravenous heparin: apropos of 12 cases]. | 2011 | 3048545 | |
| radiosensitivity of cloned permanent murine bone marrow stromal cell lines: nonuniform effect of low dose rate. | the x-irradiation biology of supportive stromal cells of the bone marrow microenvironment was investigated by using cloned permanent cell lines that were established from hematopoietically active murine long-term bone marrow cultures. x-irradiation survival curves were derived for each cell line at either 120 cgy/min or clinical low dose rate (ldr) (5 cgy/min) that is used in total body irradiation protocols prior to bone marrow transplantation. four cell lines, mba-1, mba-13, 14f2.1, and d2xrii ... | 2011 | 3049129 |
| the endocrine responses to general anesthesia. | 2011 | 3049390 | |
| deletion of the shiga toxin gene in a chlorate-resistant derivative of shigella dysenteriae type 1 that retains virulence. | we used a probe specific for detecting the structural-gene sequences of shiga toxin to analyze the genetic nature of toxin synthesis in mutant derivatives of shigella dysenteriae type 1. a chlorate-resistant (chl) mutant (725-78) of s. dysenteriae type 1 strain 3818t, which had retained virulence but had lost production of high levels of cytotoxic activity associated with shiga toxin synthesis, contained a complete deletion of the shiga toxin structural-gene sequences. these structural-gene sequ ... | 1988 | 3049838 |
| botulinum toxin a-induced protective ptosis in corneal disease. | botulinum toxin a produces a temporary, flaccid ptosis when injected into the levator palpebrae superioris muscle. the resulting protective ptosis was used to aid healing in 21 cases of indolent ulceration, and, prophylactically, in 4 cases of neuroparalytic keratitis. of the indolent ulcers, 90% healed completely. in all but one case, the cornea was covered completely by the lid and complete ptosis was produced in 75% of cases in an average of 3.6 days, lasting for 16 days on average before rec ... | 2011 | 3050691 |
| office evaluation and management of the shoulder impingement syndrome. | the shoulder impingement syndrome is the most common cause of shoulder pain. a complete office evaluation can be aided by a printed exam sheet. careful consideration of the differential diagnosis is important, often employing the xylocaine injection test. the impingement syndrome responds to conservative treatment the majority of the time. long follow-up determines the need for an invasive evaluation or surgical treatment. | 2011 | 3050719 |
| [pro-allergy role of infection. a component of the mode of reacting]. | no organism from the cradle lives germ-free or allergen-free. the modalities by which infections facilitate conditions of abnormal reactivity, in particular respiratory asthma and allergy to cow milk proteins, are examined. 216 asthmatic children and 50 infants with rotavirus enteritis have been considered. infections, besides representing stimuli directly projected on the immune system, also constitute factors which more generally influence the way of reacting. | 1988 | 3050902 |
| [tecto-thalamo-telencephalic visual system of the brain in 13-day-old chick embryos]. | light and electron microscopic studies have been made of the nervous tissue in three parts of the tecto-thalamo-telencephalic visual system--i.e. tectum opticum, nucleus rotundus of thalamus and ectostriatum of telencephalon--of 13-day chick embryos. neuroblasts and neurones at various stages of differentiation were described together with various types of synaptic and nonsynaptic intercellular contacts in the neuropil of these brain structures. heterochronous maturation of these parts of the vi ... | 2010 | 3051806 |
| [differentiation of virulent strains of shigella and entero-invasive escherichia from their avirulent variants using modified indirect immunoenzyme analysis]. | the data obtained in this investigation confirm that the modified indirect enzyme immunoassay (eia) permits the differentiation of virulent bacteria of the genus shigella and enteroinvasive escherichia (group 1), regularly containing virulence plasmids with a molecular weight of 120-140 md, from their avirulent variants which have lost these plasmids (group 2). the ratio of the optic density (od) values of the positive control samples (the od of group 1) to the od values of the negative ones (th ... | 1988 | 3051808 |
| the persistence of shigella flexneri in the united states: increasing role of adult males. | the annual reported isolation rate of shigella flexneri decreased from 1964 to 1973, but has remained constant since then at 1 per 100,000. between 1975 and 1985, the median age of males from whom s. flexneri was isolated rose from 5 to 26 years. during this time, the isolation rate of s. flexneri rose more than five-fold among men, did not change in adult women, and decreased in children. by 1985, 23 per cent of reported s. flexneri isolates came from men aged 20-49. increased male homosexual t ... | 1988 | 3052115 |
| the first international standard for antibodies to double stranded dna. | this paper announces the availability of the first international standard for anti-double-stranded dna (anti-dsdna). the material, coded wo/80, was obtained after recalcification of plasma taken from a patient with systemic lupus erythematosus. vials were filled with 500 microliters serum and freeze dried. the serum contains no other autoantibodies in measurable quantities. the vials should be stored at -20 degrees c. the standard should be used for establishing national, regional, or local stan ... | 2011 | 3052322 |
| the epidemiology of shigellosis in israel. | analysis of shigella isolates referred for serotyping to the central shigella reference laboratory has revealed a slight predominance of s. sonnei (47%) over s. flexneri (44%) in israel. in female patients, the predominance of s. sonnei isolates was more accentuated (54 v. 37.5% for s. flexneri). on the other hand, an increased incidence of s. flexneri was observed in military personnel (59 v. 32% for s. sonnei), and even more so in the arab population (69 v. 19%). incidence peaked in summer (ju ... | 1988 | 3052334 |
| the ecology and pathogenicity of urease-producing bacteria in the urinary tract. | urease activity is a physiological function of many bacteria that enables these organisms to utilize urea as a source of nitrogen. the association of ureolytic bacteria with human or animal hosts varies widely from a commensal relationship as demonstrated with skin microflora, a symbiotic relationship in the gastrointestinal tract, to a pathogenic relationship in the urinary tract. since similar or identical species of bacteria such as staphylococcus aureus are found in all three environments, t ... | 1988 | 3053050 |
| the ecology and pathogenicity of urease-producing bacteria in the urinary tract. | urease activity is a physiological function of many bacteria that enables these organisms to utilize urea as a source of nitrogen. the association of ureolytic bacteria with human or animal hosts varies widely from a commensal relationship as demonstrated with skin microflora, a symbiotic relationship in the gastrointestinal tract, to a pathogenic relationship in the urinary tract. since similar or identical species of bacteria such as staphylococcus aureus are found in all three environments, t ... | 1988 | 3053050 |
| shigella flexneri bacteraemia in post partum period. | 1988 | 3053638 | |
| [a case using plasmapheresis in treating infectious toxicosis in young infants]. | 1988 | 3054796 | |
| [stability of shigella flexneri attenuated strain 2a 77 ade- rif-r after cultivation in a fermenter and lyophilization]. | the method for the cultivation of s. flexneri attenuated strain 2a 77 ade- rifr in a fermenter with the use of casein fermentative broth containing 200-250 mg% of amino nitrogen has been developed. no changes in the properties of the initial strain have been found after its cultivation in a fermenter or after the lyophilization of the cultures grown in a fermenter. after cultivation in casein fermentative broth with 100 mg% of amino nitrogen the presence of s-r dissociation has been established, ... | 1988 | 3055763 |
| gadolinium-dtpa-enhanced mr imaging of spinal neoplasms: preliminary investigation and comparison with unenhanced spin-echo and stir sequences. | unenhanced t1- and t2-weighted spin-echo, short inversion time inversion recovery (stir), and gadolinium-dtpa (gd-dtpa)-enhanced spin-echo and stir imaging techniques were used in 20 patients as part of a multicenter study to assess the safety and efficacy of gd-dtpa in spinal imaging. five patients had normal mr scans. of those with lesions, both gd-dtpa-enhanced t1-weighted spin-echo and unenhanced stir scans improved detection and evaluation of spinal tumors over conventional spin-echo method ... | 2011 | 3055895 |
| relation of silent myocardial ischemia to ventricular arrhythmias and sudden death. | silent myocardial ischemia is common in the clinical spectrum of coronary disease. ambulatory electrocardiographic monitoring has provided the most objective evidence of silent ischemia, but the phenomenon has also been detected in patients with coronary artery disease through analysis of exercise-induced ischemic st-segment alterations, scintigraphic myocardial perfusion defects and left ventricular wall motion abnormalities. silent myocardial ischemia frequently occurs in patients with stable ... | 2011 | 3055916 |
| [phase ii study of carboplatin in cervical carcinoma]. | a phase ii multi-center study of carboplatin for cervical carcinoma was carried out in 22 institutes throughout japan. the patients registered consisted of 40 women with 39 cervical carcinomas and an endometrial carcinoma, of whom 31 were evaluable. carboplatin was administered intravenously every 4 weeks at a dose of 400 mg/m2, in cases with no prior therapies and/or p.s. 0-1, and 300 mg/m2 in cases with prior therapies and/or p.s. 2-3. the overall response rate of 31 evaluable cases was 19.4% ... | 2011 | 3056277 |
| vagal innervation techniques in the treatment of panic disorder. | 2011 | 3056393 | |
| seventy years on. | 2008 | 3057455 | |
| characterization of invasion plasmid antigen genes (ipabcd) from shigella flexneri. | the large invasion plasmid of shigella flexneri m9ot-w was used to generate recombinant plasmids carrying the ipaa, -b, -c, and -d genes, whose products are associated with the entry of the bacteria into colonic epithelial cells. complete dna sequences of ipab, -c, and -d were determined. the proteins predicted (62, 42, and 37 kda, respectively) from the nucleotide sequences lack a signal-peptide sequence. hydrophilic segments of the ipab and ipac proteins were found to overlap known epitopic do ... | 1988 | 3057506 |
| persistent shigellosis. | shigella usually causes a self-limited infection which untreated lasts on average seven days (range one to 30 days). we report a patient who had persistent symptoms for 10 months caused by shigella flexneri which was finally identified on cultures from a rectal swab. | 1988 | 3058558 |
| affinity chromatography of reca protein and reca nucleoprotein complexes on reca protein-agarose columns. | we have analyzed the nature of reca protein-reca protein interactions using an affinity column prepared by coupling reca protein to an agarose support. when radiolabeled soluble proteins from escherichia coli are applied to this column, only the labeled reca protein from the extract was selectively retained and bound tightly to the affinity column. efficient binding of purified 35s-labeled reca protein required mg2+, and high salt did not interfere with the binding of reca protein to the column. ... | 1988 | 3058701 |
| [substantiation of permissible migration quantities of beryllium in sanitary-chemical studies of materials designated for contact with food products]. | 2011 | 3060413 | |
| [etiologic structure of bacterial dysentery in the ussr in 1983-1985]. | the etiological structure of dysentery in the ussr in 1983-1985 is characterized. sonne dysentery was found to prevail in the territories with adequate water supply, while dysentery caused by shigella flexneri prevailed at the territories with unsatisfactory water supply. s. dysenteriae and s. boydii were found to play a limited role in the etiology of dysentery. in the presence of global pandemic, an increase in the isolation rate of s. dysenteriae i in the ussr is observed. the data on the bio ... | 1988 | 3061262 |
| a 13c-n.m.r. study of some shigella flexneri o-polysaccharides. | 13c-n.m.r. data for some shigella flexneri o-polysaccharides are reported. it is concluded that the chemical shifts observed for the linear shigella flexneri type y o-polysaccharide are similar to those calculated by adding substituent shifts, obtained from disaccharides with similar stereochemistry around the glycosidic linkage, to the chemical shifts for the monomers. the influence of substitution with alpha-d-glucopyranosyl and/or o-acetyl groups in different positions of this polysaccharide ... | 1988 | 3061647 |
| epidemiological aspects of tardive dyskinesia. | although there continues to be some debate as to the extent to which neuroleptic drug treatment is either necessary or sufficient to produce abnormal involuntary movements in various psychiatric populations, the consensus at present is that neuroleptics do play a major role in producing, precipitating or evoking abnormal involuntary movements. no doubt a variety of other factors play a critical role in predisposing particular individuals to the development of the condition. numerous prevalence s ... | 2010 | 3063513 |
| [pathogenetic significance of virulence-associated genes in shigella flexneri]. | 1988 | 3063851 | |
| interaction between vestibulocollic and cervicocollic reflexes: automatic compensation of reflex gain by muscle afferents. | 1988 | 3064144 | |
| [electron microscopic study of the interaction of virulent and avirulent shigella flexneri strains with the macrophages of murine peritoneal exudate]. | the electron microscopic dynamic study of the interaction of s. flexneri virulent strain 2a and s. flexneri avirulent strain, genetically related to strain 2a and having the same antigenic structure, with peritoneal macrophages of cba mice at an early period showed differences in the cytotoxic action of these strains. even at the early period of the interaction between macrophages and virulent shigellae structural changes were observed in macrophages: ruptures in the membranes of parasitophore p ... | 1988 | 3064506 |
| [scientific bases of epidemiologic surveillance of intestinal infections]. | 1988 | 3064508 | |
| microcystic adenoma of the pancreas: report of a case and review of the literature. | 2011 | 3064760 | |
| the use of dc electric fields to promote regeneration in the mammalian nervous system. | 2010 | 3064792 |