Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| subcellular localization of ipac, an invasion plasmid antigen of shigella dysenteriae type 1 and its in-vitro binding capability to mammalian cells. | invasion plasmid antigen c (ipac), a 45-kda protein encoded by an invasion plasmid of shigella, is associated with the invasion of epithelial cells by the bacteria. invasive strains of s. dysenteriae type 1 secreted more proteins into the extracellular environment than a non-invasive strain and secreted more ipac protein. an anti-ipac mouse monoclonal antibody was used as a probe to determine the subcellular localization of ipac and its involvement in invasion of mammalian cells. immunogold labe ... | 1995 | 24414917 |
| [effects of lamivudine on growth of intestinal characteristic bacteria by microcalorimetry in vitro]. | the study is aimed to investigate the effect of lamivudine on growth and metabolism of three intestinal characteristic bacteria (namely, bifidobacterium adolescentis, escherichia coli and shigella dysenteriae). the growth condition of the three bacteria was quantitatively evaluated by microcalorimetry with four characteristic parameters of the thermal power-time curves, including the growth rate constant (k), thermal power (p), time to peak (t) and calorific value (q). the results showed that th ... | 2013 | 24417087 |
| an environmental escherichia albertii strain, dm104, induces protective immunity to shigella dysenteriae in guinea pig eye model. | the environmental escherichia albertii strain dm104, which cross-reacts serologically with shigella dysenteriae was assessed for pathogenic properties, immunogenicity, and protective efficacy in different animal models to evaluate it as a vaccine candidate against s. dysenteriae, which causes the severe disease, shigellosis. the dm104 isolate was found to be non-invasive and did not produce any entero- or cyto-toxins. the strain also showed negative results in the mouse lethal activity assay. th ... | 2014 | 24452425 |
| new cationic antimicrobial peptide screened from boiled-dried anchovies by immobilized bacterial membrane liposome chromatography. | an efficient immobilized bacterial membrane liposome chromatography method was used to screen potential antimicrobial peptides from boiled-dried anchovies. a novel cationic antimicrobial peptide (apep10) was successfully isolated by one-step chromatography. the sequence of apep10 was identified as glarclagtl by matrix-assisted laser desorption/ionization quadrupole time-of-flight tandem mass spectrometry (maldi-q-tof ms). the antimicrobial activity assessment indicated that apep10 inhibited the ... | 2014 | 24499191 |
| [antibacterial effect of tjiliwung river water on shigella shigae in connection with the presence of corresponding antagonists and bacteriophages]. | 1950 | 24539386 | |
| [duration of survival time of shigella dysenteriae in feces]. | 1953 | 24543444 | |
| [fermentative characteristics of collection of culture of shigella dysenteriae newcastle]. | 1953 | 24543449 | |
| some inadequacies of culture transfer data as indices of drug resistance rates. | 1956 | 24544153 | |
| [effect of bacteriophage on resistance of flexner's bacillus to antibiotics]. | 2016 | 24544299 | |
| [intramuscular administration of biomycin and terramycin in experimental dysenterial infection]. | 2014 | 24544302 | |
| [biomycin and levomycethin in prevention and therapy of experimental dysenterial intoxication]. | 2014 | 24544304 | |
| [stability of changed properties of streptomycin-resistant strain of shigella flexneri after prolonged preservation]. | 2014 | 24544446 | |
| [effect of streptomycin and of chlortetracycline on catalase and on certain dehydrases in escherichia coli and in shigella dysenteriae]. | 2014 | 24544605 | |
| [effect of combined antibiotics of the tetracycline series with bacteriophage on shigella dysenteriae]. | 2014 | 24544824 | |
| rapid sanger sequencing of the 16s rrna gene for identification of some common pathogens. | conventional sanger sequencing remains time-consuming and laborious. in this study, we developed a rapid improved sequencing protocol of 16s rrna for pathogens identification by using a new combination of sybr green i real-time pcr and sanger sequencing with fta® cards. to compare the sequencing quality of this method with conventional sanger sequencing, 12 strains, including three kinds of strains (1 reference strain and 3 clinical strains, which were previously identified by biochemical tests) ... | 2014 | 24551186 |
| comparison of anti-bacterial activity of three types of di-o-caffeoylquinic acids in lonicera japonica flowers based on microcalorimetry. | the anti-bacterial activities of three types of di-o-caffeoylquinic acids (dicqas) in lonicera japonica flowers, a traditional chinese medicine (tcm), on bacillus shigae growth were investigated and compared by microcalorimetry. the three types of dicqas were 3, 4-di-o-caffeoylquinic acid (3, 4-dicqa), 3, 5-di-o-caffeoylquinic acid (3, 5-dicqa), and 4, 5-di-o-caffeoylquinic acid (4, 5-dicqa). some qualitative and quantitative information of the effects of the three dicqas on metabolic power-time ... | 2014 | 24636060 |
| chemical composition, antioxidant and antimicrobial activity of essential oil and extracts of tragopogon graminifolius, a medicinal herb from iran. | tragopogon graminifolius dc., family compositae, is widely consumed as a green vegetable in the west of iran and for the treatment of gastrointestinal and hepatic ailments. in this study, the chemical composition of the essential oil from t. graminifolius aerial parts was evaluated by gas chromatography and gas chromatography mass spectrometry. moreover, antioxidant and antimicrobial activity of the essential oil and various extracts of t. graminifolius were determined. fifty-eight compounds rep ... | 2014 | 24660479 |
| [microcalorimetric investigation on the interaction of six alkaloids from rhizoma coptidis]. | how to identify active constituents of traditional chinese medicines (tcms) and study their interactions are key problems in the development of tcms. the inhibitory effect of six alkaloids from rhizoma coptidis (rc) on shigella dysenteriae (s. dysenteria) growth had been investigated by microcalorimetry in this study. main active constituents of rc were confirmed by comparing their contributions to the bacteriostatic effect, and the interactions among active constituents were further researched. ... | 2013 | 24689238 |
| hemolytic uremic syndrome associated with acinetobacter hemolyticus. | shiga toxin-producing escherichia coli and shigella dysenteriae have been associated with bloody diarrhea and hemolytic uremic syndrome (hus) in humans. however, there have been only a couple of reports describing bloody diarrhea associated with acinetobacter spp. and there are no reports of these bacteria causing hus in children. here, we report the case of a nine-month-old boy with bloody diarrhea who developed non-oliguric renal failure. the clinical and laboratory findings supported the diag ... | 2014 | 24828697 |
| faecal pollution loads in the wastewater effluents and receiving water bodies: a potential threat to the health of sedibeng and soshanguve communities, south africa. | the discharge of untreated or inadequately treated effluents has been identified among the activities responsible for the spread of a wide range of potentially infectious agents. the aim of this study was to determine whether inadequate treatment of wastewater and the faecal pollution load of effluents and receiving water bodies in sedibeng district and soshanguve peri-urban area of the tshwane metropolitan municipality could be a potential threat to the health of the surrounding communities. va ... | 2014 | 24838129 |
| genomic analysis of the emergence of 20th century epidemic dysentery. | shigella dysenteriae type 1 (sd1) causes recurrent epidemics of dysentery associated with high mortality in many regions of the world. sd1 infects humans at very low infectious doses (10 cfu), and treatment is complicated by the rapid emergence of antibiotic resistant sd1 strains. sd1 is only detected in the context of human infections, and the circumstances under which epidemics emerge and regress remain unknown. | 2014 | 24886041 |
| passive immunity with multi-serotype heat-killed shigellae in neonatal mice. | the short- and long-term passive protective efficacy of a mixture of heat-killed cells of six serogroups/serotypes of shigella strains (shigella dysenteriae 1, s. flexneri 2a, s. flexneri 3a, s. flexneri 6, s. boydii 4, and s. sonnei) were studied in neonatal mice. neonatal mice from immunized dams exhibited significant short- and long-term passive protection against individual challenge by each of the six shigella strains. high igg and iga titers against the lipopolysaccharide from each of the ... | 2014 | 24909404 |
| new high-affinity monoclonal antibodies against shiga toxin 1 facilitate the detection of hybrid stx1/stx2 in vivo. | shiga toxin-producing e. coli (stec) are a group of common and potentially deadly intestinal pathogens expressing shiga toxin (stx) as a primary virulence factor. of the two types of stx, stx2 is responsible for more severe symptoms during infection, while stx1 is almost identical to the shiga toxin from shigella dysenteriae, a ubiquitous pathogen in developing countries. although antibodies against stx1 have been reported, few have reached the affinity needed for assembling highly sensitive imm ... | 2014 | 24914553 |
| shigella isolates from the global enteric multicenter study inform vaccine development. | shigella, a major diarrheal disease pathogen worldwide, is the target of vaccine development. the global enteric multicenter study (gems) investigated burden and etiology of moderate-to-severe diarrheal disease in children aged <60 months and matched controls without diarrhea during 3 years at 4 sites in africa and 3 in asia. shigella was 1 of the 4 most common pathogens across sites and age strata. gems shigella serotypes are reviewed to guide vaccine development. | 2014 | 24958238 |
| activity of antibacterial, antiviral, anti-inflammatory in compounds andrographolide salt. | andrographolide sulfonic acid sodium salt (ass) was synthesized to increase the the solubility of andrographolide in aqueous solution. we have studied its pharmacological effect of antibiosis, anti-inflammatory and immunoregulation. cylinder-plate method was used to study ass׳s in vitro antibacterial activity, and its protection for mice infected by staphylococcus aureus and shigella dysenteriae. various inflammation models, including the auricular edema induced by xylene in mice, cmc-na induced ... | 2014 | 24998876 |
| genome sequencing, annotation and comparative genomic analysis of shigella dysenteriae strain sd1d. | shigellosis is an acute form of gastroenteritis caused by the bacteria belonging to the genus shigella. it is the most common cause of morbidity and mortality in children. shigella belongs to the family enterobactericeae, which is a gram-negative and rod shaped bacterium. in the present study, we report the draft genome of shigella dysenteriae strain sd1d, which was isolated from the stool sample of a healthy individual. | 2014 | 25028600 |
| use of plant extracts to control and treat ab5 enterotoxin-related diarrhea. | plants contain a broad spectrum of small molecules with potential antimicrobial properties. here, we review the antimicrobial activities of plant extracts against enterotoxic bacteria encoding ab5 toxins, including vibrio cholerae, shigella dysenteriae and enterotoxic escherichia coli strains. several plant extracts have strong antimicrobial effects and the potential to boost oral rehydration therapy, which is the first line of treatment for acute diarrhea. | 2014 | 25033656 |
| effect of shiga toxin and its subunits on cytokine induction in different cell lines. | shiga toxins (stxs) are bacterial virulence factors produced by shigella dysenteriae serotype 1 and escherichia coli strains. stxs are critical factors for the development of diseases such as severe bloody diarrhea and hemolytic uremic syndrome. additionally, stxs trigger the secretion of pro- inflammatory cytokines and chemokines, particularly in monocytes or macrophages. the inflammatory cytokines result in the modulation of the immune system, local inflammations and enhancement of cytotoxicit ... | 2014 | 25035861 |
| lethal synergy involving bicyclomycin: an approach for reviving old antibiotics. | one way to address the growing problem of antimicrobial resistance is to revive old compounds that may have intrinsic lethal activity that is obscured by protective factors. bicyclomycin is an old inhibitor of the rho transcription terminator that by itself shows little rapid lethal activity. however, bicyclomycin participates in bacteriostatic synergy, which raises the possibility that conditions for lethal synergy may exist, perhaps through a suppression of protective factors. | 2014 | 25085655 |
| shigella: a highly virulent and elusive pathogen. | despite a significant decrease in shigella-related mortality, shigellosis continues to carry a significant burden of disease worldwide, particularly in asia and africa. shigella is a highly virulent pathogen comprised of four major species with numerous subtypes. shigella dysenteriae and shigella flexneri infections are predominant in resource-limited settings. clinical presentations range from mild watery diarrhea to severe dysentery with systemic complications such as electrolyte imbalance, se ... | 2014 | 25110633 |
| effect of water storage in silver container on the viability of enteric bacterial pathogens. | silver is one of the heavy metals traditionally played major role in the human life. it is used in the form of ornaments or as containers to store or drink water and other consumable liquids. the study was designed to observe the effect of water storage in silver containers on enteric pathogens. three sets of sterile silver, stainless steel and glass metal screw capped containers were filled with non-chlorinated sterilized well water. one each of the three sets was inoculated with enteric pathog ... | 2012 | 25145073 |
| antibacterial and antifungal activity of holothuria leucospilota isolated from persian gulf and oman sea. | emergence of antimicrobial resistance toward a number of conventional antibiotics has triggered the search for antimicrobial agents from a variety of sources including the marine environment. | 2014 | 25147657 |
| proteomes of pathogenic escherichia coli/shigella group surveyed in their host environments. | proteomic studies on shigella dysenteriae, shigella flexneri, enterohemorrhagic escherichia coli and uropathogenic e. coli (upec) are reviewed. upec causes infections in the urogenital tract, whereas the other species colonize and, to varying degrees, invade the intestinal tract. type iii secretion systems used to breach the mucosal barrier by the intestinal pathogens revealed distinct expression patterns in different host environments. dynamic adaptations to changes in nutrient availability and ... | 2014 | 25163594 |
| total phenolic content and antibacterial activity of five plants of labiatae against four foodborne and some other bacteria. | the aim of this study was to evaluate the antibacterial effects of thymus vulgaris, thymus caramanicus, zataria multiflora, ziziphora clinopodioides and ziziphora tenuior against four foodborne and four other bacteria including staphylococcus aureus, shigella dysenteriae, salmonella typhimurium, escherichia coli, staphylococcus epidermidis, bacillus subtilis, mrsa and pseudomona aeruginosa and measuring the amount of total phenolics of the plants. the extracts were prepared by maceration method. ... | 2014 | 25237351 |
| hijacking bacterial glycosylation for the production of glycoconjugates, from vaccines to humanised glycoproteins. | glycosylation or the modification of a cellular component with a carbohydrate moiety has been demonstrated in all three domains of life as a basic post-translational process important in a range of biological processes. this review will focus on the latest studies attempting to exploit bacterial n-linked protein glycosylation for glycobiotechnological applications including glycoconjugate vaccine and humanised glycoprotein production. the challenges that remain for these approaches to reach full ... | 2014 | 25244672 |
| isolation and development of bioluminescent reporter phages for bacterial dysentery. | shigellosis is a significant cause of morbidity and mortality worldwide, most notably amongst children. moreover, there is a global increase in the occurrence of multidrug-resistant isolates, including the epidemic and pandemic shigella dysenteriae type 1 strain. we developed a bioluminescent reporter phage assay to facilitate detection and simultaneously determine antibiotic susceptibility. a shigella flexneri phage (shfl25875) was isolated from environmental wastewater and characterized by dna ... | 2015 | 25252629 |
| molecular characterization of serologically atypical provisional serovars of shigella isolates from kolkata, india. | during 2000-2004, 13 shigella strains that were untypable by commercially available antisera were isolated from children <5 years of age with acute diarrhoea in kolkata. these strains were subsequently identified as shigella dysenteriae provisional serovar 204/96 (n = 3), shigella dysenteriae provisional serovar e23507 (n = 1), shigella dysenteriae provisional serovar i9809-73 (n = 1), shigella dysenteriae provisional serovar 93-119 (n = 1), shigella flexneri provisional serovar 88-893 (n = 6) a ... | 2014 | 25261061 |
| clinical isolates of shiga toxin 1a-producing shigella flexneri with an epidemiological link to recent travel to hispañiola. | shiga toxins (stx) are cytotoxins involved in severe human intestinal disease. these toxins are commonly found in shigella dysenteriae serotype 1 and shiga-toxin-producing escherichia coli; however, the toxin genes have been found in other shigella species. we identified 26 shigella flexneri serotype 2 strains isolated by public health laboratories in the united states during 2001-2013, which encode the shiga toxin 1a gene (stx1a). these strains produced and released stx1a as measured by cytotox ... | 2014 | 25271406 |
| antibacterial activity of ethyl acetate and aqueous extracts of mentha longifolia l. and hydroalcoholic extract of zataria multiflora boiss. plants against important human pathogens. | to determine the potential antibacterial activity of ethyl acetate and aqueous extracts from mentha longifolia l. (m. longifolia) and hydroalcoholic extract of zataria multiflora boiss. (z. multiflora) against important human pathogens. | 2014 | 25312118 |
| an in vitro antibacterial and antifungal effects of cadmium(ii) complexes of hexamethyltetraazacyclotetradecadiene and isomers of its saturated analogue. | to evaluate the antibacterial and antifungal effects of cadmium(ii) complexes with hexamethyltetraazacyclotetradecadiene ligands. | 2014 | 25312179 |
| shigella dysenteriae modulates bmp pathway to induce mucin gene expression in vivo and in vitro. | mucosal epithelial cells in the intestine act as the first line of host defense against pathogens by increasing mucin production for clearance. despite this fact, the underlying molecular mechanisms by which shigella dysenteriae transduce mucin gene expression remain poorly defined. the goal of this study was to determine the role of bone morphogenetic protein (bmp) pathway in mucin gene expression during s. dysenteriae infection. in this study we demonstrate that s. dysenteriae activates bmp si ... | 2014 | 25365201 |
| the preliminary assessment of anti-microbial activity of hplc separated components of kirkia wilmsii. | most communities in developing countries rely on traditional medicines for the treatment of diseases. in south africa, the limpopo province, within the lebowakgomo district, uses tuberous roots of kirkia wilmsii, after infusion in water for the treatment of a wide range of diseases by sotho communities. | 2014 | 25371593 |
| synthesis of an aminooxy derivative of the trisaccharide globotriose gb3. | the synthesis of α-aminooxy trisaccharide moiety [α-d-gal-(1,4)-β-d-gal-(1,4)-β-d-glc-α-aminooxy], related to the cell surface globotriaosylceramide (gb3) receptor of the b subunit of the ab5 shiga toxin of shigella dysenteriae, has been synthesized for the first time in 11 steps with a 15% overall isolated yield. a highlight of this work entails utilizing chemically compatible synthetic transformations, including those related to glycosylation, incorporative of the succinimidyl moiety as a prec ... | 2014 | 25382930 |
| evaluation of antibacterial and anthelmintic activities with total phenolic contents of piper betel leaves. | the study was conducted to investigate the antibacterial and anthelmintic activities and to determine total phenolic contents of methanolic extract of piper betel leaves. | 2014 | 25386394 |
| shiga toxin-induced apoptosis is more efficiently inhibited by dimeric recombinant hybrid-igg/iga immunoglobulins than by the parental igg monoclonal antibodies. | shiga toxin 1 (stx1) is a virulence factor of enterohaemorrhagic escherichia coli strains such as o157:h7 and shigella dysenteriae. to prevent entry of stx1 from the mucosal surface, an immunoglobulin a (iga) specific for stx1 would be useful. due to the difficulty of producing iga monoclonal antibodies (mab) against the binding subunit of stx1 (stx1b) in mice, we took advantage of recombinant technology that combines the heavy chain variable region from stx1b-specific igg1 mab and the fc region ... | 2014 | 25469594 |
| molecular characterization of multidrug-resistant shigella spp. of food origin. | shigella spp. are the causative agents of food-borne shigellosis, an acute enteric infection. the emergence of multidrug-resistant clinical isolates of shigella presents an increasing challenge for clinicians in the treatment of shigellosis. several studies worldwide have characterized the molecular basis of antibiotic resistance in clinical shigella isolates of human origin, however, to date, no such characterization has been reported for shigella spp. of food origin. in this study, we characte ... | 2015 | 25485847 |
| shiga toxin (stx) classification, structure, and function. | shiga toxin (stx) is one of the most potent bacterial toxins known. stx is found in shigella dysenteriae 1 and in some serogroups of escherichia coli (called stx1 in e. coli). in addition to or instead of stx1, some e. coli strains produce a second type of stx, stx2, that has the same mode of action as stx/stx1 but is antigenically distinct. because subtypes of each toxin have been identified, the prototype toxin for each group is now designated stx1a or stx2a. the stxs consist of two major subu ... | 2014 | 25530917 |
| characterization of new myoviridae bacteriophage wz1 against multi-drug resistant (mdr) shigella dysenteriae. | shigella dysenteriae is a normal inhabitant of the human gastrointestinal tract, but sometimes it causes severe infection known as shigellosis (bacillary dysentery). bacteriophages are considered very safe and effective agents for controlling bacterial infections and contaminations. in this study, we describe the isolation and characterization of bacteriophage wz1, isolated from waste water which inhibits the growth of s. dysenteriae. phage wz1 showed maximum stability at 37 °c and was stable up ... | 2015 | 25557472 |
| antibacterial efficacy of five medicinal plants against multidrug-resistant enteropathogenic bacteria infecting under-5 hospitalized children. | to evaluate in vitro antibacterial effectiveness of five medicinal plants used by an indian aborigine, against 8 multidrug-resistant (mdr) enteropathogenic bacteria isolated from clinical samples of under-5 hospitalized children. | 2015 | 25609371 |
| shigella outer membrane protein pssp-1 is broadly protective against shigella infection. | in developing countries, shigella is a primary cause of diarrhea in infants and young children. although antibiotic therapy is an effective treatment for shigellosis, therapeutic options are narrowing due to the emergence of antibiotic resistance. thus, preventive vaccination could become the most efficacious approach for controlling shigellosis. we have identified several conserved protein antigens that are shared by multiple shigella serotypes and species. among these, one antigen induced cros ... | 2015 | 25651919 |
| virulence variations in shigella and enteroinvasive escherichia coli using the caenorhabditis elegans model. | shigella species and enteroinvasive escherichia coli (eiec) belong to the same species genetically, with remarkable phenotypic and genomic similarities. shigella is the main cause of bacillary dysentery with around 160 million annual cases, while eiec generally induces a milder disease compared to shigella. this study aimed to determine virulence variations between shigella and eiec using the nematode caenorhabditis elegans as a model host. caenorhabditis elegans killing- and bacterial colonizat ... | 2015 | 25673655 |
| the genome of shigella dysenteriae strain sd1617 comparison to representative strains in evaluating pathogenesis. | we sequenced and analyzed shigella dysenteriae strain sd1617 serotype 1 that is widely used as model strain for vaccine design, trials and research. a combination of next-generation sequencing platforms and assembly yielded two contigs representing a chromosome size of 4.34 mb and the large virulence plasmid of 177 kb. this genome sequence is compared with other shigella genomes in order to understand gene complexity and pathogenic factors. | 2015 | 25743074 |
| prevalence of enteropathogenic bacteria in treated effluents and receiving water bodies and their potential health risks. | the failure of wastewater treatment plants to produce effluents of a high microbiological quality is a matter of great concern in terms of water resource pollution. a more serious concern is that this water source is used by communities in developing countries for multiple purposes, which include drinking, recreation and agriculture. the current study investigated the prevalence and potential health risks of enteropathogenic bacteria (salmonella typhimurium, shigella dysenteriae and vibrio chole ... | 2015 | 25777950 |
| characterization of indigenous lactobacillus strains for probiotic properties. | probiotics are defined as adequate amount of live microorganisms able to confer health benefits on the host. currently, most commercially available probiotic products in the market belong to genera lactobacillus. traditional dairy products are usually rich source of lactobacillus strains with significant health benefits. in order to evaluate the probiotic potential of these bacteria, it is essential to assess their health benefits, efficacy, and safety. | 2015 | 25793099 |
| antibacterial, antifungal, and insecticidal potentials of oxalis corniculata and its isolated compounds. | oxalis corniculata is a common medicinal plant widely used against numerous infectious diseases. the agrochemical potential of methanolic extract, n-hexane, chloroform, ethyl acetate, and n-butanol fractions were assessed to measure the antibacterial, antifungal, and insecticidal activities of the plant. the crude, chloroform, and n-butanol soluble fractions showed excellent activities against escherichia coli, shigella dysenteriae, salmonella typhi, and bacillus subtilis but have no activity ag ... | 2015 | 25873973 |
| antibacterial and antispasmodic activities of a dichloromethane fraction of an ethanol extract of stem bark of piliostigma reticulatum. | this study presents the antispasmodic and antibacterial properties of an ethanol extract and fractions the of stem bark of piliostigma reticulatum. | 2017 | 25883517 |
| detection of e. coli o157:h7 and shigella dysenteriae toxins in clinical samples by pcr-elisa. | shiga toxin producing bacteria are potential causes of serious human disease such as hemorrhagic colitis, severe inflammations of ileocolonic regions of gastrointestinal tract, thrombocytopenia, septicemia, malignant disorders in urinary ducts, hemolytic uremic syndrome (hus). shiga toxin 1 (stx1), shiga toxin 2 (stx2), or a combination of both are responsible for most clinical symptoms of these diseases. a lot of methods have been developed so far to detect shiga toxins such as cell culture, el ... | 2015 | 25911087 |
| a novel method for screening of potential probiotics for high adhesion capability. | to screen for potential probiotics with high adhesion capability, a chemostat model-based cultured human feces and denaturing gradient gel electrophoresis methods were applied, and the adhesion capability of the isolates was evaluated in vitro and in vivo. lactobacillus plantarum (hm218749), lactobacillus reuteri (eu547310), and enterococcus faecalis (hm218543) were isolated from the slime on the chemostat wall, as these organisms could grow better at 37°c in an anaerobic environment and could r ... | 2015 | 25912863 |
| high mean water vapour pressure promotes the transmission of bacillary dysentery. | bacillary dysentery is an infectious disease caused by shigella dysenteriae, which has a seasonal distribution. external environmental factors, including climate, play a significant role in its transmission. this paper identifies climate-related risk factors and their role in bacillary dysentery transmission. harbin, in northeast china, with a temperate climate, and quzhou, in southern china, with a subtropical climate, are chosen as the study locations. the least absolute shrinkage and selectio ... | 2015 | 25946209 |
| in vitro antibacterial and in vivo cytotoxic activities of grewia paniculata. | grewia paniculata (family: malvaceae) has been used to treat inflammation, respiratory disorders and fever. it is additionally employed for other health conditions including colds, diarrhea and as an insecticide in bangladesh. the aim of the present study was to investigate the antibacterial and cytotoxic activities of different extracts of grewia paniculata. | 0 | 25949950 |
| prevalence of shiga toxin-producing shigella species isolated from french travellers returning from the caribbean: an emerging pathogen with international implications. | shiga toxins (stxs) are potent cytotoxins that inhibit host cell protein synthesis, leading to cell death. classically, these toxins are associated with intestinal infections due to stx-producing escherichia coli or shigella dysenteriae serotype 1, and infections with these strains can lead to haemolytic-uraemic syndrome. over the past decade, there has been increasing recognition that stx is produced by additional shigella species. we recently reported the presence and expression of stx genes i ... | 2015 | 25980352 |
| synthesis, chiral separation, absolute configuration assignment, and biological activity of enantiomers of retro-1 as potent inhibitors of shiga toxin. | the shiga toxin (stx) family is composed of related protein toxins produced by the bacteria shigella dysenteriae and certain pathogenic strains of e. coli. no effective therapies for stx intoxication have been developed yet. however, inhibitors that act on the intracellular trafficking of these toxins may provide new options for the development of therapeutic strategies. this study reports the synthesis, chromatographic separation, and pharmacological evaluation of the two enantiomers of retro-1 ... | 2015 | 26033849 |
| expression, purification and immunological characterization of recombinant shiga toxin a subunit. | shiga toxin family comprises toxins belonging to two major groups, stx1 and stx2, produced by the bacteria shigella dysenteriae and some strains of escherichia coli. shiga toxins are the leading cause of diarrhea associated with life threatening hemolytic uremic syndrome (hus). stxa is a ribosome inactivating protein (rip) which inhibits the protein synthesis in most species of prokaryotes and eukaryotes. an in vitro expression system has not been reported to produce full- length biological acti ... | 2015 | 26149398 |
| safety and immunogenicity of a candidate bioconjugate vaccine against shigella dysenteriae type 1 administered to healthy adults: a single blind, partially randomized phase i study. | shigellae cause severe disease in endemic countries, especially in children. several efficacy trials have been conducted with candidate vaccines against shigellae, but the lack of protection, the safety concerns, or manufacturing challenges hindered successful market approval. conjugated vaccines have been shown to be safe and effective for different pathogens (i.e., neisseria meningitidis, shigella pneumonia, haemophilus influenzae). the bio-conjugation technology, exploited here for the shigel ... | 2015 | 26162850 |
| inhibition of pathogenic bacterial growth on excision wound by green synthesized copper oxide nanoparticles leads to accelerated wound healing activity in wistar albino rats. | an impaired wound healing is one of the major health related problem in diabetic and non-diabetic patients around the globe. the pathogenic bacteria play a predominant role in delayed wound healing, owing to interaction in the wound area. in our previous work we developed green chemistry mediated copper oxide nanoparticles using ficus religiosa leaf extract. in the present study we make an attempt to evaluate the anti-bacterial, and wound healing activity of green synthesized copper oxide nanopa ... | 2015 | 26194977 |
| [blood groups - minuses and pluses. do the blood group antigens protect us from infectious diseases?]. | human blood can be divided into groups, which is a method of blood classification based on the presence or absence of inherited erythrocyte surface antigens that can elicit immune response. according to the international society of blood transfusion, there are 341 blood group antigens collected in 35 blood group systems. these antigens can be proteins, glycoproteins or glycosphingolipids, and function as transmembrane transporters, ion channels, adhesion molecules or receptors for other proteins ... | 2015 | 26206987 |
| in vitro and in vivo examination of anticolonization of pathogens by lactobacillus paracasei fj861111.1. | very limited information exists on the exclusion of pathogens by probiotics in the gut of the host challenged with pathogens. in this study, we tested probiotic characteristics in vitro and anticolonization ability of lactobacillus paracasei fj861111.1 in mice infected with selected pathogenic microorganisms. the in vitro results indicated that l. paracasei fj861111.1 had a high survival in acidic conditions at ph 2.5 and bile salt concentration at 0.3%, and strong inhibition ability against com ... | 2015 | 26254535 |
| intracellular and membrane-damaging activities of methyl gallate isolated from terminalia chebula against multidrug-resistant shigella spp. | shigella spp. (shigella dysenteriae, shigella flexneri, shigella boydii and shigella sonnei) cause bacillary dysentery (shigellosis), which is characterized by bloody mucous diarrhoea. although a variety of antibiotics have been effective for treatment of shigellosis, options are becoming limited due to globally emerging drug resistance. in the present study, in vitro antibacterial activity of methyl gallate (mg) isolated from terminalia chebula was determined by performing mic, minimal bacteric ... | 2015 | 26272388 |
| expression, purification and immunological characterization of recombinant shiga toxin a subunit. | shiga toxin family comprises toxins belonging to two major groups, stx1 and stx2, produced by the bacteria shigella dysenteriae and some strains of escherichia coli. shiga toxins are the leading cause of diarrhea associated with life threatening hemolytic uremic syndrome (hus). stxa is a ribosome inactivating protein (rip) which inhibits the protein synthesis in most species of prokaryotes and eukaryotes. an in vitro expression system has not been reported to produce full-length biological activ ... | 2015 | 26282729 |
| exoelectrogens leading to precise reduction of graphene oxide by flexibly switching their environment during respiration. | reduced graphene oxide (rgo) has been prepared by a simple, cost-effective, and green route. in this work, graphene oxide (go) has been reduced using gram-negative facultative anaerobe s. dysenteriae, having exogenic properties of electron transfer via electron shuttling. apparently, different concentrations of go were successfully reduced with almost complete mass recovery. an effective role of lipopolysaccharide has been observed while comparing rgo reduced by s. dysenteriae and s. aureus. it ... | 2015 | 26288348 |
| purification and characterization of a shigella conjugate vaccine, produced by glycoengineering escherichia coli. | shigellosis remains a major cause of diarrheal disease in developing countries and causes substantial morbidity and mortality in children. glycoconjugate vaccines consisting of bacterial surface polysaccharides conjugated to carrier proteins are the most effective vaccines for controlling invasive bacterial infections. nevertheless, the development of a multivalent conjugate vaccine to prevent shigellosis has been hampered by the complex manufacturing process as the surface polysaccharide for ea ... | 2016 | 26353918 |
| novel strain of shigella dysenteriae serotype 7 from india. | we describe a strain of shigella dysenteriae serotype 7 which had novel biochemical and genetic characters. unlike other s. dysenteriae, it produced gas, fermented mannitol, was a late-lactose fermenter and harboured the set 1a and set 1b genes. the significance of such atypical strains is that they are difficult to identify. if such strains are missed, they could prove to be a serious public health problem because the infectious dose is very low and they may harbour integrons contributing to dr ... | 2015 | 26442152 |
| shiga toxins: potent poisons, pathogenicity determinants, and pharmacological agents. | the shiga toxins (stxs), also known as vero toxins and previously called shiga-like toxins, are a family of potent protein synthesis inhibitors made by shigella dysenteriae type 1 and some serogroups of escherichia coli that cause bloody diarrhea in humans. stxs act as virulence factors for both s. dysenteriae and e. coli and contribute to the disease process initiated by those organisms both directly and indirectly. a handful of methods exist for toxin purification, and the toxins can now even ... | 2005 | 26443514 |
| immunization of mice with a live transconjugant shigella hybrid strain induced th1 and th17 cell-mediated immune responses and confirmed passive protection against heterologous shigellae. | an avirulent, live transconjugant shigella hybrid (ltshδstx) strain was constructed in our earlier study by introducing a plasmid vector, ppr1347, into a shiga toxin gene deleted shigella dysenteriae 1. three successive oral administrations of ltshδstx to female adult mice produced comprehensive passive heterologous protection in their offspring against challenge with wild-type shigellae. production of no and different cytokines such asil-12p70, il-1β and il-23 in peritoneal mice macrophages ind ... | 2016 | 26478541 |
| stx-producing shigella species from patients in haiti: an emerging pathogen with the potential for global spread. | shiga toxins (stx) are commonly produced by shigella dysenteriae serotype 1 and stx-producing escherichia coli. however, the toxin genes have been detected in additional shigella species. we recently reported the emergence of stx-producing shigella in travelers in the united states and france who had recently visited hispaniola (haiti and the dominican republic). in this study, we confirm this epidemiological link by identifying stx-producing shigella from haitian patients attending clinics near ... | 2015 | 26484357 |
| controlling the cytokine storm in severe bacterial diarrhoea with an oral toll-like receptor 4 antagonist. | shigella dysenteriae causes the most severe of all infectious diarrhoeas and colitis. we infected rhesus macaques orally and also treated them orally with a small and non-absorbable polypropyletherimine dendrimer glucosamine that is a toll-like receptor-4 (tlr4) antagonist. antibiotics were not given for this life-threatening infection. six days later, the clinical score for diarrhoea, mucus and blood was 54% lower, colon interleukin-8 and interleukin-6 were both 77% lower, and colon neutrophil ... | 2016 | 26496144 |
| slow relaxation of shape and orientational texture in membrane gel domains. | gel domains in lipid bilayers are structurally more complex than fluid domains. growth dynamics can lead to noncircular domains with a heterogeneous orientational texture. most model membrane studies involving gel domain morphology and lateral organization assume the domains to be static. here we show that rosette shaped gel domains, with heterogeneous orientational texture and a central topological defect, after early stage growth, undergo slow relaxation. on a time scale of days to weeks domai ... | 2015 | 26501924 |
| synthesis, density functional theory (dft), urease inhibition and antimicrobial activities of 5-aryl thiophenes bearing sulphonylacetamide moieties. | a variety of novel 5-aryl thiophenes 4a-g containing sulphonylacetamide (sulfacetamide) groups were synthesized in appreciable yields via pd[0] suzuki cross coupling reactions. the structures of these newly synthesized compounds were determined using spectral data and elemental analysis. density functional theory (dft) studies were performed using the b3lyp/6-31g (d, p) basis set to gain insight into their structural properties. frontier molecular orbital (fmos) analysis of all compounds 4a-g wa ... | 2015 | 26556326 |
| development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid detection of francisella tularensis. | francisella tularensis is a potential biowarfare/bioterrorism agent and zoonotic pathogen that causes tularemia; thus, surveillance of f. tularensis and first-level emergency response using point-of-care testing (poct) are essential. the upt-lf poct assay was established to quantitatively detect f. tularensis within 15 min, and the sensitivity of the assay was 10(4) cfu · ml(-1) (100 cfu/test). the linear quantitative range covered five orders of magnitude, and the coefficients of variation were ... | 2015 | 26608358 |
| comparative analysis of the shiga toxin converting bacteriophage first detected in shigella sonnei. | here we report the first complete nucleotide sequence of a shiga toxin (stx) converting phage from a shigella sonnei clinical isolate that harbors stx1 operon, first identified in the chromosome of shigella dysenteriae type 1. the phage named shigella phage 75/02 stx displayed podoviridae morphology. it proved to be transferable to escherichia coli k-12 strains, and cytotoxicity of the lysogenized strains was demonstrated in vero cell cultures. genomic analysis revealed that the prophage genome ... | 2016 | 26616675 |
| evaluation of anti-bacterial effects of some novel thiazole and imidazole derivatives against some pathogenic bacteria. | bacterial resistance to antibiotics has motivated the researchers to evaluate the novel anti-bacterial compounds such as some thiazole and imidazole derivatives. thereby, in this work, we investigated the anti-bacterial effects of one new thiazole and two new imidazole derivatives on bacillus cereus, listeria monocytogenes, escherichia coli, salmonella typhimurium, proteus mirabilis and shigella dysenteriae. | 2015 | 26719785 |
| evaluation of antibacterial activity of plant mediated cao nanoparticles using cissus quadrangularis extract. | an assessment of antibacterial activity of greenly synthesized nanoparticles using aqueous stem extract of cissus quadrangularis was carried out. the synthesized nanoparticles were characterized by uv-vis spectroscopy, sem, xrd, ftir and further subjected for antibacterial activity against the pathogens staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae and vibrio cholerae. the sem photograph represents cubic and hexagon ... | 2016 | 26723000 |
| antimicrobial properties of a potential probiotic lactobacillus from thai newborn feces. | probiotics are increasingly used to treat infectious diarrhea and antibiotic-associated diarrhea. many probiotic bacteria are classified in general such as lactobacillus and are able to colonize the gastrointestinal tracts of infants. | 2015 | 26817219 |
| factors affecting inducible expression of outer membrane protein a (ompa) of shigella dysenteriae type-1 in lactococcus lactis using nisin inducible controlled expression (nice). | potential use of lactococcus lactis (l. lactis) as a heterologous protein expression host as well as for delivery of multiple therapeutic proteins has been investigated extensively using nisin inducible controlled expression (nice) system. optimum inducible expression of heterologous protein by nice system in l. lactis depends on multiple factors. to study the unexplored role of factors affecting heterologous protein expression in l. lactis using nice, the present study outlines the optimization ... | 2016 | 26843700 |
| shiga toxin (stx) classification, structure, and function. | shiga toxin (stx) is one of the most potent bacterial toxins known. stx is found in shigella dysenteriae 1 and in some serogroups of escherichia coli (called stx1 in e. coli). in addition to or instead of stx1, some e. coli strains produce a second type of stx, stx2, that has the same mode of action as stx/stx1 but is antigenically distinct. because subtypes of each toxin have been identified, the prototype toxin for each group is now designated stx1a or stx2a. the stxs consist of two major subu ... | 2014 | 26904753 |
| shiga toxin 1-producing shigella sonnei infections, california, united states, 2014-2015. | shiga toxins (stx) are primarily associated with shiga toxin-producing escherichia coli and shigella dysenteriae serotype 1. stx production by other shigellae is uncommon, but in 2014, stx1-producing s. sonnei infections were detected in california. surveillance was enhanced to test s. sonnei isolates for the presence and expression of stx genes, perform dna subtyping, describe clinical and epidemiologic characteristics of case-patients, and investigate for sources of infection. during june 2014 ... | 2016 | 26982255 |
| shiga toxins as multi-functional proteins: induction of host cellular stress responses, role in pathogenesis and therapeutic applications. | shiga toxins (stxs) produced by shiga toxin-producing bacteria shigella dysenteriae serotype 1 and select serotypes of escherichia coli are primary virulence factors in the pathogenesis of hemorrhagic colitis progressing to potentially fatal systemic complications, such as hemolytic uremic syndrome and central nervous system abnormalities. current therapeutic options to treat patients infected with toxin-producing bacteria are limited. the structures of stxs, toxin-receptor binding, intracellula ... | 2016 | 26999205 |
| screening of furanone in cucurbita melo and evaluation of its bioactive potential using in silico studies. | the work presented here attempts to screen for the presence of 4-hydroxy-2,5-dimethyl-3(2h)-furanone in fruits and also any bioactive potential present in the fruit extracts. curcurbita melo was selected for the study, and the fruit was crushed, filtered and extracted with ethyl acetate as the solvent. the resulting extract was subjected to disk diffusion test using kirby bauer method for checking its antimicrobial potential. melon extract showed promising results against different clinical path ... | 2016 | 27013507 |
| two novel antimicrobial peptides from skin venoms of spadefoot toad megophrys minor. | amphibian skin contains rich bioactive peptides. especially, a large amount of antimicrobial peptides have been identified from amphibian skin secretions. antimicrobial peptides display potent cytolytic activities against a range of pathogenic bacteria and fungi and play important defense roles. no antimicrobial peptides have been reported from toads belonging to the family of pelobatidae. in this work, two novel antimicrobial peptides (megin 1 and megin 2) were purified and characterized from t ... | 2016 | 27114317 |
| shigella diversity and changing landscape: insights for the twenty-first century. | shigella is a pathovar of escherichia coli comprising four groups, shigella flexneri, shigella sonnei, shigella dysenteriae, and shigella boydii, each of them, with the exception of s.sonnei, comprising several serotypes. shigella accounts for the majority of dysentery causing infections occurring world-wide each year. recent advancements in the shigella field have led to a better understanding of the molecular mechanisms underlying host epithelial cell invasion and immune cell function manipula ... | 2016 | 27148494 |
| [microbiological approach to a possible infective endocarditis case caused by aggregatibacter actinomycetemcomitans]. | aggregatibacter (actinobacillus) actinomycetemcomitans, a small, gram-negative coccobacillus that grows slow and fastidious, is generally colonized in the oral cavity. it is a rarely seen bacterium because of the difficulty of isolation but it can be a causative agent for dental infections and infective endocarditis (ie) particularly in the persons having prosthetic heart valves. in this report, a possible ie case caused by a.actinomycetemcomitans in a patient with aortic valve replacement has b ... | 2016 | 27175505 |
| competitive survival of escherichia coli, vibrio cholerae, salmonella typhimurium and shigella dysenteriae in riverbed sediments. | studies on the survival of bacterial enteric pathogens in riverbed sediments have mostly focused on individual organisms. reports on the competitive survival of these pathogens in riverbed sediments under the same experimental setup are limited. we investigated the survival of escherichia coli, salmonella enterica ser. typhimurium, vibrio cholerae and shigella dysenteriae in riverbed sediments of the apies river. experiments were performed in flow chambers containing three sediment types and con ... | 2016 | 27193001 |
| a two-step method for extraction of lipopolysaccharide from shigella dysenteriae serotype 1 and salmonella typhimurium: an improved method for enhanced yield and purity. | bacterial lipopolysaccharide (lps) has been widely used as an antigen and adjuvant in immunological applications. amongst the methods developed for extraction of lps, hot phenol extraction (hpe) method is the gold standard. however, the hpe method provides poor yield of lps (~4.5% by weight), is associated with relatively higher impurities of proteins and nucleic acids, and the acidic hot phenol can cause a degradative effect on lps. in this work a two-step extraction (tse) method was developed ... | 2016 | 27208662 |
| occurrence of potential bacterial pathogens and their antimicrobial susceptibility patterns isolated from herbal medicinal products sold in different markets of gondar town, northwest ethiopia. | background. the world health organization estimates that about 80% of the world's population uses herbal medicine to treat various illnesses as means of primary healthcare. however, during preparation, herbal plants may be exposed to contamination by potential pathogens, and this may lead to infections. the aim of this study was to determine bacterial contamination of herbal medicinal products and to assess the antibiotic susceptibility pattern of the isolated bacteria. methods. a cross-sectiona ... | 2016 | 27299154 |
| pathogen-associated porin turns il-10 competent b-1a cells toward proinflammatory cytokine response. | shigellosis is a major problem in the developing countries causing mortality and morbidity particularly among the children. shigella spp. harbours the epithelial cells of the human colon to infect the host and spread the disease. we analyzed the response of b-1a cells, the major component of the mucosal immune system to porin of shigella dysenteriae type 1. we show that porin while proliferating b-1a cells, deplete siglec-g, the inhibitory molecule present on b-1a cells. adjuvanticity of porin h ... | 2016 | 27498179 |
| erratum to: genome sequencing, annotation and comparative genomic analysis of shigella dysenteriae strain sd1d. | [this corrects the article doi: 10.1186/1757-4749-6-28.]. | 2016 | 27516810 |
| isolation of pathogenic bacteria from hospital staff apparel in nigeria. | a survey of bacteria contamination of hospital staff apparel in use in anambra state, nigeria, was carried out to determine the extent of contamination by clinically important bacteria. of a total of 125 swab samples of hospital staff apparel, 72 (58%) showed bacterial contamination including 32 (70%) of 46 samples from hand gloves, 28 of 45 (62%) samples from protective gowns, and 12 of 34 (35%) samples from face-shields. the potentially pathogenic bacteria isolated were salmonella spp, proteus ... | 2005 | 27529000 |
| global phylogeography and evolutionary history of shigella dysenteriae type 1. | together with plague, smallpox and typhus, epidemics of dysentery have been a major scourge of human populations for centuries(1). a previous genomic study concluded that shigella dysenteriae type 1 (sd1), the epidemic dysentery bacillus, emerged and spread worldwide after the first world war, with no clear pattern of transmission(2). this is not consistent with the massive cyclic dysentery epidemics reported in europe during the eighteenth and nineteenth centuries(1,3,4) and the first isolation ... | 2016 | 27572446 |
| a review on sero diversity and antimicrobial resistance patterns of shigella species in africa, asia and south america, 2001-2014. | shigella, gram negative bacterium, is responsible for shigellosis/bacillary dysentery. it is a global concern although it predominates in developing countries. these are shigella dysenteriae, shigella flexneri, shigella boydii and shigella sonnei. drug resistance by shigella species is another headache of the world. therefore; this study aimed to review distribution of shigella serogroups and their antimicrobial patterns carried out in africa, asia and south america. | 2016 | 27576729 |
| characterization of beauty salon wastewater from kwame nkrumah university of science and technology, kumasi, ghana, and its surrounding communities. | due to the increase in students' population over the years, the kwame nkrumah university of science and technology (knust), kumasi, ghana, and its surrounding communities have seen an increase in the number of beauty salons. the assessment of the quality of salon wastewater has received little attention, as a potential source of environmental and public health hazard, due to the lack of literature on this issue. the main aim of this study is to assess wastewater effluent characteristics in knust ... | 2016 | 27594788 |