Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| investigation on the agonistic and antagonistic biological activities of synthetic chlamydia lipid a and its use in in vitro enzymatic assays. | the synthetic 1,4'-bisphosphorylated penta-acyl and tetra-acyl lipid a structures representing the major molecular species of natural chlamydial lipid a were tested for their endotoxic activities as measured by interleukin-8 release from human embryonic kidney (hek) 293 cells expressing toll-like receptor (tlr) 2 or tlr4. both compounds were unable to activate hek293 cells transiently transfected with tlr2. the penta-acyl lipid a was a weak activator of hek293 cells expressing tlr4/md-2/cd14 whe ... | 2007 | 17621554 |
| effects of salmonella enterica subsp. enterica serovar enteritidis vaccination in layer hens subjected to s. enteritidis challenge and various feed withdrawal regimens. | levels of salmonella enterica subsp. enterica serovar enteritidis infection and serum s. enteritidis antibodies after experimental s. enteritidis challenge and feed withdrawal were investigated in s. enteritidis-vaccinated and unvaccinated hens. the results were used to determine whether formalin-inactivated s. enteritidis vaccination can protect layer hens from s. enteritidis challenge during feed withdrawal periods. s. enteritidis infection rates were evaluated from cloacal swabs, eggs and org ... | 2007 | 17624693 |
| the distribution of acetohydroxyacid synthase in soil bacteria. | most bacteria possess the enzyme acetohydroxyacid synthase, which is used to produce branched-chain amino acids. enteric bacteria contain several isozymes suited to different conditions, but the distribution of acetohydroxyacid synthase in soil bacteria is largely unknown. growth experiments confirmed that escherichia coli, salmonella enterica serotype typhimurium, and enterobacter aerogenes contain isozymes of acetohydroxyacid synthase, allowing the bacteria to grow in the presence of valine (w ... | 2008 | 17624809 |
| chicken gallinacin gene cluster associated with salmonella response in advanced intercross line. | salmonella enterica serovar enteritidis is a gram-negative bacterium that negatively affects human and animal health. many eukaryotes use antimicrobial peptides (alpha-defensins, beta-defensins, gamma-defensins, and cathelicidins) in innate immune responses to fight bacterial infections. poultry gallinacins are the functional equivalents of mammalian beta-defensins. two related advanced intercross lines of chickens were analyzed for association of gallinacin genotypic variation with salmonella e ... | 2007 | 17626484 |
| molting in salmonella enteritidis-challenged laying hens fed alfalfa crumbles. i. salmonella enteritidis colonization and virulence gene hila response. | the objectives of this study were to enumerate salmonella enterica serovar enteritidis colonization in fecal, cecal, and internal organs, and to compare the level of virulence gene expression (hila) of experimentally challenged laying hens fed different dietary molt-induction regimens. twelve salmonella-free single comb leghorn hens (>50 wk old) hens were randomly assigned to each of 6 treatment groups designated based on diet in 2 trials: 1) feed withdrawal salmonella enteritidis-positive (fw+) ... | 2007 | 17626806 |
| assessing the prevalence of salmonella enterica in poultry hatcheries by using hatched eggshell membranes. | salmonella enterica causes a number of significant poultry diseases and is also a major pathogen in humans. most poultry infected by salmonella become carriers; infection may also be fatal, depending on the particular serovar and the age of the bird at infection. younger birds are more susceptible to infection by salmonella, so it is critical that hatcheries monitor birds. we developed a method to use hatched eggshell membranes (hem) to assess contamination by salmonella in poultry hatching cabi ... | 2007 | 17626809 |
| gene promoter scan methodology for identifying and classifying coregulated promoters. | a critical challenge of the postgenomic era is to understand how genes are differentially regulated. genetic and genomic approaches have been used successfully to assign genes to distinct regulatory networks in both prokaryotes and eukaryotes. however, little is known about what determines the differential expression of genes within a particular network, even when it involves a single transcription factor. the fact that coregulated genes may be differentially expressed suggests that subtle diffe ... | 2007 | 17628149 |
| phosphorylation assays of chemotaxis two-component system proteins in borrelia burgdorferi. | borrelia burgdorferi has a complex chemotaxis signal transduction system with multiple chemotaxis gene homologs similar to those found in escherichia coli and bacillus subtilis. the b. burgdorferi genome sequence encodes two chea, three chey, three chew, two cheb, two cher, but no chez genes. instead of chez, b. burgdorferi contains a different chey-p phosphatase, referred to as chex. the multiple b. burgdorferi histidine kinases (chea1 and chea2) and response regulators (chey1, chey2, and chey3 ... | 2007 | 17628153 |
| core-linked lps expression of shigella dysenteriae serotype 1 o-antigen in live salmonella typhi vaccine vector ty21a: preclinical evidence of immunogenicity and protection. | shigella dysenteriae serotype 1 (s. dysenteriae 1) causes severe shigellosis that is typically associated with high mortality. antibodies against shigella serotype-specific o-polysaccharide (o-ps) have been shown to be host protective. in this study, the rfb locus and the rfp gene with their cognate promoter regions were pcr-amplified from s. dysenteriae 1, cloned, and sequenced. deletion analysis showed that eight rfb orfs plus rfp are necessary for biosynthesis of this o-ps. a tandemly-linked ... | 2007 | 17629369 |
| emergence of multidrug-resistant salmonella enterica serovar typhi associated with a class 1 integron carrying the dfra7 gene cassette in nepal. | a total of 121 salmonella enterica serovars typhi and paratyphi a isolated from enteric fever patients at a university hospital in nepal between february 2004 and january 2006 were tested for their antimicrobial susceptibility. the occurrence and cassette content of integrons as well as the molecular mechanisms of resistance among the multidrug-resistant (mdr) s. typhi were evaluated. thirty-nine percent of the isolates were susceptible to all the antimicrobial agents tested. seven of the s. typ ... | 2007 | 17629465 |
| tricarballylate catabolism in salmonella enterica. the tcub protein uses 4fe-4s clusters and heme to transfer electrons from fadh2 in the tricarballylate dehydrogenase (tcua) enzyme to electron acceptors in the cell membrane. | tricarballylate, a citrate analogue, is considered the causative agent of grass tetany, a ruminant disease characterized by acute magnesium deficiency. although the normal rumen flora cannot catabolize tricarballylate, the gram-negative enterobacterium salmonella enterica can. an operon dedicated to tricarballylate utilization (tcuabc) present in this organism encodes all functions required for tricarballylate catabolism. tricarballylate is converted to the cis-aconitate in a single oxidative st ... | 2007 | 17630784 |
| isolation of salmonella enterica serotype newport from a partly ruptured splenic abscess in a traveler returning from zanzibar. | salmonella enterica subsp. enterica serotype newport is a pathogen of growing importance because of its epidemic spread in dairy cattle and increasing rate of antimicrobial resistance. human infections, however, are rare. we report a case of a splenic abscess in a young traveler returning from east africa. | 2007 | 17634302 |
| multiplex, bead-based suspension array for molecular determination of common salmonella serogroups. | we report the development and evaluation of a salmonella o-group-specific bio-plex assay to detect the six most common serogroups in the united states (b, c(1), c(2), d, e, and o13) plus serotype paratyphi a. the assay is based on rfb gene targets directly involved in o-antigen biosynthesis; it can be completed 45 min post-pcr amplification. the assay correctly and specifically identified 362 of 384 (94.3%) isolates tested in comparison to traditional serotyping. seventeen isolates (4.4%) produc ... | 2007 | 17634307 |
| salmonella pathogenicity island 4-mediated adhesion is coregulated with invasion genes in salmonella enterica. | salmonella pathogenicity island 4 (spi4) encodes a type i secretion system and the cognate substrate protein, siie. we have recently demonstrated that siie is a giant nonfimbrial adhesin involved in the adhesion of salmonella enterica serovar typhimurium to polarized epithelial cells. we also observed that under in vitro culture conditions, the synthesis and secretion of siie coincided with the activation of salmonella invasion genes. these observations prompted us to investigate the regulation ... | 2007 | 17635868 |
| plasmid-mediated quinolone resistance determinant qnrs1 detected in salmonella enterica serovar corvallis strains isolated in denmark and thailand. | 2007 | 17635876 | |
| the effect of methylation on dna replication in salmonella enterica serovar typhimurium. | the dna adenine methylase of salmonella typhimurium methylates adenine at gatc sequences. strains deficient in this methylase are not well transformed by methylated plasmids, but unmethylated plasmids transform them at high frequencies. hemimethylated daughter molecules accumulate after the transformation of dam(-) strains with fully methylated plasmids, suggesting that hemimethylation prevents dna replication. it will also be shown that plasmids isolated from dam(-) bacteria are hemimethylated ... | 2007 | 17637438 |
| cloning, nucleotide sequencing, and analysis of the acrab-tolc efflux pump of enterobacter cloacae and determination of its involvement in antibiotic resistance in a clinical isolate. | enterobacter cloacae is an emerging clinical pathogen that may be responsible for nosocomial infections. management of these infections is often difficult, owing to the high frequency of strains that are resistant to disinfectants and antimicrobial agents in the clinical setting. multidrug efflux pumps, especially those belonging to the resistance-nodulation-division family, play a major role as a mechanism of antimicrobial resistance in gram-negative pathogens. in the present study, we cloned a ... | 2007 | 17638702 |
| a multiplex polymerase chain reaction method for rapid identification of citrobacter freundii and salmonella species, including salmonella typhi. | salmonella enterica is one of the most common enteric pathogens worldwide. conventional methods of isolation of salmonella strains take 4-7 days to complete, are laborious and require substantial manpower. we devised a polymerase chain reaction (pcr) method that simultaneously uses three pairs of specific primers to detect inv, spv, and via genes of salmonella. | 2007 | 17639162 |
| change of serotype pattern of group d non-typhoidal salmonella isolated from pediatric patients in southern taiwan. | group d non-typhoidal salmonella infection is increasing in taiwan. this study aimed to investigate the changing serotypes and antibiotic resistance of childhood group d salmonella infection. | 2007 | 17639164 |
| extraintestinal focal infections in adults with salmonella enterica serotype choleraesuis bacteremia. | salmonella enterica serotype choleraesuis, which is associated with severe human infections and multidrug resistance, poses a serious problem in taiwan. the aim of the study was to investigate the epidemiology and clinical features of s. choleraesuis bacteremia. | 2007 | 17639165 |
| gas-forming splenic abscess due to salmonella enterica serotype enteritidis in a chronically hemodialyzed patient. | we describe a diabetic patient who was chronically hemodialyzed due to end-stage renal disease and developed a gas-forming splenic abscess and bacteremia caused by salmonella enterica serotype enteritidis. fever persisted despite urgent splenectomy and intravenous ceftriaxone and metronidazole for 14 days. he recovered completely after intravenous ciprofloxacin/metronidazole treatment for a further 14 days. the isolate was susceptible to ciprofloxacin and ceftriaxone and did not exhibit extended ... | 2007 | 17639171 |
| [prevalence of the bacteria causing diarrea in a rosario hospital, argentina]. | bacterial agents causing diarrea in patients attended in a hospital of rosario, argentina. the frequency of bacterial agents causing diarrhea can vary in patients of different areas, and through the time. in an epidemiological surveillance we studied 304 patients with diarrheal diseases looking for salmonella enterica, shigella spp, campylobacter spp, aeromonas spp, yersinia enterocolitica, and diarrheogenic escherichia coli. c. jejuni was isolated in 30 patients (9,9 %), salmonella in 18 (5,9 % ... | 2006 | 17639815 |
| phage typing, biotyping & antimicrobial resistance profile of salmonella enterica serotype typhi from kolkata. | kolkata and its suburbs in eastern india are known to be endemic for typhoid fever. the objective of this study was to determine phage types, biotypes and antimicrobial resistance patterns of salmonella enterica serotype typhi isolated during the period 2003-2005 from a prospective surveillance for typhoid fever in two urban slums in kolkata. | 2007 | 17642505 |
| ctx-m-14 type beta-lactamase producing salmonella enterica serovar enteritidis isolated from imported chicken meat. | 2007 | 17642544 | |
| a preliminary report on current antibiogram of salmonella enterica serotype typhi in nagpur. | 2003 | 17643050 | |
| new signaling molecules in some gram-positive and gram-negative bacteria. | a new family of putative signaling molecules having a 2(5h)-furanone configuration has been described in this work. they were released during late exponential or stationary phase in different growth media by some gram-positive bacteria, such as lactobacillus helveticus, lactobacillus plantarum, lactobacillus paraplantarum, lactobacillus sanfranciscensis, enterococcus faecalis, and a gram-negative species, i.e. salmonella enterica. a pair of 2(5h)-furanones called furanones a and b occurred in al ... | 2007 | 17643538 |
| nose-to-nose transmission of salmonella typhimurium between weaned pigs. | little attention has been paid to the possibility of transmission of salmonella in intensive pig production systems through alternate methods, such as airborne or direct nose-to-nose contact. this experimental study tested the hypothesis of nose-to-nose transmission of salmonella enterica serovars typhimurium (trial i) and agona (trial ii) in weaned pigs using stainless steel/glass isolation cabinets. in each trial, cabinet 1 (control pigs) and cabinet 2 (sentinel pigs) were connected directly t ... | 2007 | 17643873 |
| potential management of resistant microbial infections with a novel non-antibiotic: the anti-inflammatory drug diclofenac sodium. | diclofenac sodium (dc), an anti-inflammatory agent, has remarkable inhibitory action both against drug-sensitive and drug-resistant clinical isolates of various gram-positive and gram-negative bacteria. the aim of this study was to determine the ability of dc to protect mice from a virulent salmonella infection. dc injected at 1.5 microg/g and 3.0 microg/g mouse body weight significantly protected animals from the lethality of salmonella infection. as was the case for the in vitro interaction, d ... | 2007 | 17644318 |
| bacteria-generated ptdins(3)p recruits vamp8 to facilitate phagocytosis. | salmonella enterica serovar typhimurium invades non-phagocytic cells by inducing macropinocytosis. sopb is involved in modulating actin dynamics to promote salmonella-induced invasion. we report here that sopb-generated ptdins(3)p binds vamp8/endobrevin to promote efficient bacterial phagocytosis. vamp8 is recruited to salmonella-induced macropinosomes in a nocodazole-dependent, but brefeldin a-independent, manner. we found that vamp8 directly binds to and colocalizes with ptdins(3)p. the inosit ... | 2007 | 17645435 |
| stec is a salmonella kinase required for spi-2-dependent f-actin remodelling. | salmonella enterica serovar typhimurium (s. typhimurium) replicates inside mammalian cells within membrane-bound compartments called salmonella-containing vacuoles. intracellular replication is dependent on the activities of several effector proteins translocated across the vacuolar membrane by the salmonella pathogenicity island 2 (spi-2)-type iii secretion system (t3ss). this is accompanied by the formation in the vicinity of bacterial vacuoles of an f-actin meshwork, thought to be involved in ... | 2008 | 17645553 |
| prevalence and characterization of extended-spectrum cephalosporin-resistant nontyphoidal salmonella isolates in adults in saint petersburg, russia (2002-2005). | we studied the prevalence of resistance to extended-spectrum cephalosporins (esc) among 1,078 salmonella enterica isolates collected from adults admitted to botkin hospital, st. petersburg, russia, for gastroenteritis between 2002 and 2005. only two esc-resistant isolates were detected, giving a low percentage of strains resistant to esc (0.2%). one multidrug-resistant (mdr) isolate of the virchow serotype produced a ctxm-3 extended-spectrum beta-lactamase (esbl). the bla(ctx-m-3) gene was locat ... | 2007 | 17650961 |
| prevalence, antimicrobial resistance, and extended-spectrum beta-lactamases characterization of salmonella isolates in apulia, southern italy (2001-2005). | the prevalence, antimicrobial susceptibility, and production of extended-spectrum beta-lactamases (esbls) of salmonella collected from several hospitals in apulia (southern italy) were evaluated. the most common salmonella isolates were salmonella enterica serovar typhimurium (44.6%), s. enterica serovar enteritidis (33.4 %), s. enterica serovar infantis (3.2 %), s. enterica serovar typhi (1.5%), and s. enterica serovar bovismorbificans (1.5%). the other serovars accounted for less than 1% each. ... | 2007 | 17650965 |
| complete nucleotide sequence of a small qnrs1-carrying plasmid from salmonella enterica subsp. enterica typhimurium dt193. | 2007 | 17652106 | |
| geobacillus stearothermophilus lv cada gene mediates resistance to cadmium, lead and zinc in znta mutants of salmonella entérica serovar typhimurium. | salmonella entérica serovar typhimurium cells expressing the cada gene of geobacillus stearothermophilus lv exhibit a hypersensitive phenotype to cadmium chloride. deletion of the orf stm3576 from the salmonella genome resulted in cadmium, lead and zinc sensitivity, confirming that this orf is a homologue of the znta gene. the observed sensitivity was reverted upon expression of the g. stearothermophilus lv cada gene. these results indicate that the cada gene product is involved in cd, pb and zn ... | 2007 | 17657347 |
| characterization of the osmoprotectant transporter opuc from pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function. | the plant pathogen pseudomonas syringae may cope with osmotic stress on plants, in part, by importing osmoprotective compounds. in this study, we found that p. syringae pv. tomato strain dc3000 was distinct from most bacterial species in deriving greater osmoprotection from exogenous choline than from glycine betaine. this superior osmoprotection was correlated with a higher capacity for uptake of choline than for uptake of glycine betaine. of four putative osmoregulatory abc transporters in dc3 ... | 2007 | 17660277 |
| alterations in the two globular domains or in the connecting alpha-helix of bacterial ribosomal protein l9 induces +1 frameshifts. | the ribosomal 50s subunit protein l9, encoded by the gene rpli, is an elongated protein with an alpha-helix connecting the n- and c-terminal globular domains. we isolated rpli mutants that suppress the +1 frameshift mutation hisc3072 in salmonella enterica serovar typhimurium. these mutants have amino acid substitutions in the n-terminal domain (g24d) or in the c-terminal domain (i94s, a102d, g126v, and f132s) of l9. in addition, different one-base deletions in rpli altered either the final port ... | 2007 | 17660285 |
| mutations in yhit enable utilization of exogenous pyrimidine intermediates in salmonella enterica serovar typhimurium. | mutants capable of utilizing the pyrimidine biosynthetic intermediates carbamoylaspartate and dihydroorotate for growth were derived from pyrimidine auxotrophs of salmonella enterica serovar typhimurium lt2. the gain-of-function phenotypes both resulted from mutations in a single gene, yhit, the third gene of a putative four-gene operon, yhivuts, for which there is no homologous region in escherichia coli. notably, when a mutant yhit allele was transferred to a pyrimidine-requiring e. coli strai ... | 2007 | 17660412 |
| the escherichia coli arac-family regulators gadx and gadw activate gade, the central activator of glutamate-dependent acid resistance. | escherichia coli can survive ph 2 acid stress by using several acid resistance systems. the most efficient of these employs glutamate decarboxylase (gada/gadb) to consume protons, and an antiporter (gadc) to exchange the intracellular decarboxylation product for external glutamic acid. expression of the essential transcriptional activator of this system, gade, is controlled by several regulators in a hierarchical fashion. in this study, two additional activators have been identified. the arac-fa ... | 2007 | 17660422 |
| shigella flexneri phagosomal escape is independent of invasion. | infections with salmonella enterica serovar typhimurium and shigella flexneri result in mucosal inflammation in response to epithelial cell invasion and macrophage cytotoxicity. these processes are mediated by type iii secretion systems encoded in homologous virulence loci in the two species, namely, salmonella pathogenicity island 1 (spi-1), carried in the genome, and the shigella entry region (ser), carried in a large virulence plasmid. here we show that spi-1 can functionally complement a del ... | 2007 | 17664266 |
| i/st mice hypersusceptible to mycobacterium tuberculosis are resistant to m. avium. | we previously demonstrated that mice of the i/st strain are extremely susceptible to mycobacterium tuberculosis, as well as to the taxonomically distant intracellular bacteria chlamydia pneumoniae and salmonella enterica. to broaden our knowledge about the control of susceptibility to intracellular pathogens, we studied the infection caused by mycobacterium avium virulent strain 724 in a panel of inbred mouse strains and found that i/st mice are resistant to m. avium. by comparing i/st mice with ... | 2007 | 17664269 |
| a proteomic approach to study salmonella enterica serovar typhimurium putative transporter yjeh associated with ceftriaxone resistance. | mutant 6b7 of salmonella enterica serovar typhimurium has a transposon inserted in the putative transporter gene yjeh and shows a more-than-fourfold reduction in resistance to ceftriaxone. in this report we have used proteomic analysis to compare outer membrane protein profiles between this mutant and its parental strain r200. five identified proteins were found to be altered. of these proteins, the level of expression of the porin ompd was increased and those of the putative outer membrane prot ... | 2007 | 17669360 |
| role of nucleoid-associated proteins hha and h-ns in expression of salmonella enterica activators hild, hilc, and rtsa required for cell invasion. | the coordinate expression of salmonella enterica invasion genes on salmonella pathogenicity island 1 is under the control of the complex circuits of regulation that involve the arac/xyls family transcriptional activators hild, hilc, and rtsa and nucleoid-associated proteins. single-copy transcription fusions were used to assess the effects of nucleoid-associated proteins hha and h-ns on hild, hilc, and rtsa expression. the data show that all three genes, hild, hilc, and rtsa, were repressed by h ... | 2007 | 17675384 |
| isolation of salmonella enterica serovar enteritidis from houseflies (musca domestica) found in rooms containing salmonella serovar enteritidis-challenged hens. | houseflies (musca domestica) released into rooms containing hens challenged with salmonella enterica serovar enteritidis (salmonella serovar enteritidis) rapidly became contaminated with salmonella serovar enteritidis. forty to 50% of the flies were contaminated at 48 h, and the percentage increased to 50 to 70% at 4 and 7 days postexposure and then decreased to 30% at day 15. initial attempts at recovering surface organisms for culture using an aqueous rinse were largely unsuccessful, while cul ... | 2007 | 17675422 |
| cloning and transfer of the salmonella pathogenicity island 2 type iii secretion system for studies of a range of gram-negative genera. | the engineering of bacterial strains with specific phenotypes frequently requires the use of blocks or "cassettes" of genes that act together to perform a desired function. the potential benefits of utilizing type iii secretion systems in this regard are becoming increasingly realized since these systems can be used to direct interactions with host cells for beneficial purposes such as vaccine development, anticancer therapies, and targeted protein delivery. however, convenient methods to clone ... | 2007 | 17675443 |
| icsbp1/irf-8 is required for innate and adaptive immune responses against intracellular pathogens. | the chronic myeloid leukemia syndrome of the bxh-2 mouse strain (mus musculus) is caused by a recessive mutation (r294c) in the transcriptional regulator icsbp1/irf-8. in trans activation assays using an il-12p40 gene reporter construct introduced in raw 264.7 mouse macrophages, we show that the icsbp1(c294) isoform behaves as a partial loss-of-function. the icsbp1(c294) hypomorph allele appears to have a threshold effect on il-12 production, with pleiotropic consequences on resistance to differ ... | 2007 | 17675508 |
| global gene expression profile after salmonella enterica serovar enteritidis challenge in two f8 advanced intercross chicken lines. | a chicken 13k cdna microarray was used to profile global gene expression after salmonella enteritidis (se) challenge of young chickens. two f8 advanced intercross lines (ail), broiler by leghorn, and broiler by fayoumi, were studied. day-old chicks were orally inoculated with se, and spleens were harvested at day 7 or 8 post-inoculation. the se bacteria burden in the spleen was quantified. the 20% high and 20% low se burden birds within each ail and harvest time were studied by microarray. the l ... | 2007 | 17675853 |
| susceptibility of germ-free pigs to challenge with protease mutants of salmonella enterica serovar typhimurium. | salmonella protease mutants, clpp and especially htra, are candidate live oral vaccines in humans. a functional and mature immune system is, however, required to cope with them in mice. here, we test the cytokine response of highly susceptible germ-free pigs to infection with salmonella typhimurium clpp and htra mutants. cytokine levels (il-4, il-10, il-18 and ifn-gamma) were measured by elisa in plasma and washes from the terminal small bowel 24h after oral challenge. unlike the infection with ... | 2007 | 17678715 |
| immune complex-induced enhancement of bacterial antigen presentation requires fcgamma receptor iii expression on dendritic cells. | dendritic cells (dcs) are capable of initiating adaptive immune responses against infectious agents by presenting pathogen-derived antigens on mhc molecules to naïve t cells. because of their key role in priming adaptive immunity, it is expected that interfering with dc function would be advantageous to the pathogen. we have previously shown that salmonella enterica serovar typhimurium (st), is able to survive inside dcs and interfere with their function by avoiding activation of bacteria-specif ... | 2007 | 17679697 |
| cytokine gene expression in chicken cecal tonsils following treatment with probiotics and salmonella infection. | probiotics are currently employed for control of pathogens and enhancement of immune response in chickens. in this study, we investigated the underlying immunological mechanisms of the action of probiotics against colonization of the chicken intestine by salmonella enterica subsp. enterica serovar typhimurium (salmonella serovar typhimurium). birds received probiotics by oral gavage on day 1 of age and, subsequently, received salmonella serovar typhimurium on day 2 of age. cecal tonsils were rem ... | 2008 | 17681719 |
| n-lysine propionylation controls the activity of propionyl-coa synthetase. | reversible protein acetylation is a ubiquitous means for the rapid control of diverse cellular processes. acetyltransferase enzymes transfer the acetyl group from acetyl-coa to lysine residues, while deacetylase enzymes catalyze removal of the acetyl group by hydrolysis or by an nad(+)-dependent reaction. propionyl-coenzyme a (coa), like acetyl-coa, is a high energy product of fatty acid metabolism and is produced through a similar chemical reaction. because acetyl-coa is the donor molecule for ... | 2007 | 17684016 |
| assessment of contamination potential of lettuce by salmonella enterica serovar newport added to the plant growing medium. | the capacity of salmonella enterica serovar newport to contaminate romaine lettuce (lactuca sativa l. cv. nogal) via the root system was evaluated in 17-, 20-, and 33-day-old plants. apparent internalization of salmonella via the root to the above-ground parts was identified in 33- but not 17- or 20-day-old plants and was stimulated by root decapitation. leaves of lettuce plants with intact and damaged roots harbored salmonella at 500 +/- 120 and 5,130 +/- 440 cfu/g of leaf, respectively, at 2 d ... | 2007 | 17685348 |
| outbreaks where food workers have been implicated in the spread of foodborne disease. part 1. description of the problem, methods, and agents involved. | food workers in many settings have been responsible for foodborne disease outbreaks for decades, and there is no indication that this is diminishing. the committee on control of foodborne illnesses of the international association for food protection was tasked with collecting and evaluating any data on worker-associated outbreaks. a total of 816 reports with 80,682 cases were collected from events that occurred from 1927 until the first quarter of 2006. most of the outbreaks reviewed were from ... | 2007 | 17685355 |
| protective role of hemidesmus indicus r. br. root extract against salmonella typhimurium-induced cytotoxicity in int 407 cell line. | the present investigation deals with the effect of the chloroform fraction composed of sterols and fatty acids isolated from hemidesmus indicus root extract (chi) on salmonella enterica serovar typhimurium (s. typhimurium)-induced cytotoxicity in a human intestinal epithelial cell line (int 407). the optimum dose was fixed as 100 microg/ml for chi against s. typhimurium, which was quite safe for int 407 cells as the cd(50) concentration (50% cell death) of chi was determined to be 500 microg/ml ... | 2007 | 17685386 |
| part i. analysis of data gaps pertaining to salmonella enterica serotype typhi infections in low and medium human development index countries, 1984-2005. | there are only 10 contemporary, population-based studies of typhoid fever that evaluate disease incidence using blood culture for confirmation of cases. reported incidence ranged from 13 to 976/100 000 persons per year. these studies are likely to have been done preferentially in high- incidence sites which makes generalization of data difficult. only five of these studies reported mortality. of these the median (range) mortality was 0% (0-1.8%). since study conditions usually involved enhanced ... | 2008 | 17686194 |
| glutamine phosphoribosylpyrophosphate amidotransferase-independent phosphoribosyl amine synthesis from ribose 5-phosphate and glutamine or asparagine. | phosphoribosylamine (pra) is the first intermediate in the common pathway to purines and thiamine and is generated in bacteria by glutamine phosphoribosylpyrophosphate (prpp) amidotransferase (ec 2.4.2.14) from prpp and glutamine. genetic data have indicated that multiple, non-prpp amidotransferase mechanisms exist to generate pra sufficient for thiamine but not purine synthesis. here we describe the purification and identification of an activity (present in both escherichia coli and salmonella ... | 2007 | 17686772 |
| salmonella enterica serovar typhi ty21a expressing human papillomavirus type 16 l1 as a potential live vaccine against cervical cancer and typhoid fever. | human papillomavirus (hpv) vaccines based on l1 virus-like particles (vlps) can prevent hpv-induced genital neoplasias, the precursors of cervical cancer. however, most cervical cancers occur in developing countries, where the implementation of expensive vaccines requiring multiple injections will be difficult. a live salmonella-based vaccine could be a lower-cost alternative. we previously demonstrated that high hpv type 16 (hpv16)-neutralizing titers are induced after a single oral immunizatio ... | 2007 | 17687110 |
| concurrent infections in acute febrile illness patients in egypt. | we report the occurrence of concurrent infections with multiple acute febrile illness (afi) pathogens during an ongoing prospective laboratory-based surveillance in four infectious disease hospitals in urban and rural areas of egypt from june 2005 to august 2006. patients were screened for leptospira, rickettsia typhi, brucella, or salmonella enterica serogroup typhi by various methods including serology, culture, and pcr. one hundred eighty-seven of 1,510 patients (12.4%) evaluated had supporti ... | 2007 | 17690420 |
| the thiamine kinase (ycfn) enzyme plays a minor but significant role in cobinamide salvaging in salmonella enterica. | cobinamide (cbi) salvaging is impaired, but not abolished, in a salmonella enterica strain lacking a functional cobu gene. cobu is a bifunctional enzyme (ntp:adenosylcobinamide [ntp:adocbi] kinase, gtp:adenosylcobinamide-phosphate [gtp:adocbi-p] guanylyltransferase) whose adocbi kinase activity is necessary for cbi salvaging in this bacterium. inactivation of the ycfn gene in a deltacobu strain abrogated cbi salvaging. introduction of a plasmid carrying the ycfn(+) allele into a deltacobu deltay ... | 2007 | 17693493 |
| phopq-mediated regulation produces a more robust permeability barrier in the outer membrane of salmonella enterica serovar typhimurium. | the phopq two-component system of salmonella enterica serovar typhimurium produces a remodeling of the lipid a domain of the lipopolysaccharide, including the pagp-catalyzed addition of palmitoyl residue, the pmrab-regulated addition of the cationic sugar 4-aminoarabinose and phosphoethanolamine, and the lpxo-catalyzed addition of a 2-oh group onto one of the fatty acids. by using the diffusion rates of the dyes ethidium, nile red, and eosin y across the outer membrane, as well as the susceptibi ... | 2007 | 17693506 |
| recombining population structure of plesiomonas shigelloides (enterobacteriaceae) revealed by multilocus sequence typing. | plesiomonas shigelloides is an emerging pathogen that is widespread in the aquatic environment and is responsible for intestinal diseases and extraintestinal infections in humans and other animals. virtually nothing is known about its genetic diversity, population structure, and evolution, which severely limits epidemiological control. we addressed these questions by developing a multilocus sequence typing (mlst) system based on five genes (fusa, leus, pyrg, recg, and rpob) and analyzing 77 epid ... | 2007 | 17693512 |
| molecular characterization of the thi3 gene involved in thiamine biosynthesis in zea mays: cdna sequence and enzymatic and structural properties of the recombinant bifunctional protein with 4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate) kinase and thiamine monophosphate synthase activities. | a thiamine biosynthesis gene, thi3, from maize zea mays has been identified through cloning and sequencing of cdna and heterologous overexpression of the encoded protein, thi3, in escherichia coli. the recombinant thi3 protein was purified to homogeneity and shown to possess two essentially different enzymatic activities of hmp(-p) [4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate)] kinase and tmp (thiamine monophosphate) synthase. both activities were characterized in terms of basic kineti ... | 2007 | 17696876 |
| sopd acts cooperatively with sopb during salmonella enterica serovar typhimurium invasion. | the intracellular bacterial pathogen, salmonella enterica serovar typhimurium (s. typhimurium), causes disease in a variety of hosts. to invade and replicate in host cells, these bacteria subvert host molecular machinery using bacterial proteins, called effectors, which they translocate into host cells using specialized protein delivery systems. one of these effectors, sopd, contributes to gastroenteritis, systemic virulence and persistence of s. typhimurium in animal models of infection. recent ... | 2007 | 17696999 |
| molecular characterisation of multidrug-resistant salmonella enterica serovar typhimurium isolates from gomel region, belarus. | this study describes the characterisation by pulsed-field gel electrophoresis (pfge), multilocus variable number tandem repeat analysis (mlva) typing and antimicrobial resistance profiles of 35 salmonella enterica serovar typhimurium isolates, mostly from infections in children who acquired an infection outside hospitals in the gomel region of belarus. thirty-one isolates were highly similar according to pfge and mlva typing, were multidrug-resistant, including resistance to ceftiofur, and harbo ... | 2007 | 17697002 |
| identification of the salmonella enterica serotype typhimurium sipa domain responsible for inducing neutrophil recruitment across the intestinal epithelium. | in human intestinal disease induced by salmonella enterica serotype typhimurium (s. typhimurium) transepithelial migration of polymorphonuclear leukocytes (pmns) rapidly follows attachment of the bacteria to the epithelial apical membrane. previously, we have shown that the s. typhimurium effector protein, sipa, plays a pivotal role in signalling epithelial cell responses that lead to the transepithelial migration of pmns. thus, the objective of this study was to determine the functional domain ... | 2007 | 17697195 |
| regional ifngamma expression is insufficient for efficacious control of food-borne bacterial pathogens at the gut epithelial barrier. | ifngamma is critical for host defence against various food-borne pathogens including salmonella enterica and listeria monocytogenes, the causative agents of salmonellosis and listeriosis, respectively. we investigated the impact of regional ifngamma expression at the intestinal epithelial barrier on host invasion by salmonellae and listeriae following oral challenge. transgenic mice (ifngamma-gut), generated on an ifngamma knock-out (ko) background, selectively expressed ifngamma in the gut driv ... | 2007 | 17698562 |
| lipopolysaccharide from salmonella enterica activates nf-kappab through both classical and alternative pathways in primary b lymphocytes. | lipopolysaccharides (lps) are potent polyclonal b-lymphocyte activators. recently, we have shown that lps inhibits both spontaneous and drug-induced apoptosis in mature b lymphocytes, through cytosolic retention of bax, a proapoptotic protein of the bcl-2 family, by preventing its translocation to mitochondria. research within the last few years has revealed that members of the nf-kappab transcription factor regulate cell viability by activating genes involved in mitochondrion-dependent apoptosi ... | 2007 | 17698569 |
| constitutive acid sphingomyelinase enhances early and late macrophage killing of salmonella enterica serovar typhimurium. | we have identified acid sphingomyelinase (asm) as an important player in the early and late anti-salmonella activity of macrophages. a functional asm participated in the killing activity of macrophages against wild-type salmonella enterica serovar typhimurium. the role of asm in early macrophage killing of salmonella appears to be linked to an active nadph phagocyte oxidase enzymatic complex, since the flavoprotein inhibitor diphenyleneiodonium not only blocked a productive respiratory burst but ... | 2007 | 17698574 |
| comparative analysis of inchi2 plasmids carrying blactx-m-2 or blactx-m-9 from escherichia coli and salmonella enterica strains isolated from poultry and humans. | salmonella enterica bla(ctx-m-2) and bla(ctx-m-9) plasmid backbones from isolates from belgium and france were analyzed. the bla(ctx-m-2-)plasmids from both human and poultry isolates were related to the inchi2 papec-o1-r plasmid, previously identified in the united states in avian escherichia coli strains; the bla(ctx-m-9) plasmids were closely related to the inchi2 r478 plasmid. | 2007 | 17698627 |
| improving live attenuated bacterial carriers for vaccination and therapy. | live attenuated bacteria are well established as vaccines. thus, their use as carriers for prophylactic and therapeutic macromolecules is a logical consequence. here we describe several experimental applications of bacteria to carry heterologous macromolecules into the murine host. first, listeria monocytogenes are described that are able to transfer eukaryotic expression plasmids into host cells for gene therapy. high multiplicities of infection are still required for efficient gene transfer an ... | 2008 | 17702649 |
| induction of rpos degradation by the two-component system regulator rsta in salmonella enterica. | bacterial survival in diverse and changing environments relies on the accurate interplay between different regulatory pathways, which determine the design of an adequate adaptive response. the proper outcome depends on a precise gene expression profile generated from the finely tuned and concerted action of transcriptional factors of distinct regulatory hierarchies. salmonella enterica serovar typhimurium harbors multiple regulatory systems that are crucial for the bacterium to cope with harsh e ... | 2007 | 17704217 |
| influence of lactic acid bacteria on longevity of caenorhabditis elegans and host defense against salmonella enterica serovar enteritidis. | this study aimed to develop a convenient model to investigate the senescence of host defenses and the influence of food and nutrition. a small soil nematode, caenorhabditis elegans, was grown for 3 days from hatching on a lawn of escherichia coli op50 as the normal food source, and subsequently some of the nematodes were fed lactic acid bacteria (lab). the life spans of worms fed lab were significantly longer than the life spans of those fed op50. to investigate the effect of age on host defense ... | 2007 | 17704266 |
| ability of bacteriophages isolated from different sources to reduce salmonella enterica serovar enteritidis in vitro and in vivo. | salmonella enterica serovar enteritidis-lysing bacteriophages isolated from poultry or human sewage sources were used to reduce salmonella enteritidis in vitro and in experimentally infected chicks. cocktails of 4 different bacteriophages obtained from commercial broiler houses (cb4ø) and 45 bacteriophages from a municipal wastewater treatment plant (wt45ø) were evaluated. in experiment 1, an in vitro crop assay was conducted with selected bacteriophage concentrations (10(5) to 10(9) pfu/ml) to ... | 2007 | 17704377 |
| trends in salmonella enterica serotypes isolated from human, food, animal, and environment in tunisia, 1994-2004. | the present study was conducted to investigate, for the first time in tunisia, the current trends in salmonella enterica serotypes in tunisia (human, food, animal, and environment) which would help to improve the control and prevention of salmonella infections. | 2007 | 17706289 |
| chicken cecum immune response to salmonella enterica serovars of different levels of invasiveness. | day-old chicks are very susceptible to infections with salmonella enterica subspecies. the gut mucosa is the initial site of host invasion and provides the first line of defense against the bacteria. to study the potential of different s. enterica serovars to invade the gut mucosa and trigger an immune response, day-old chicks were infected orally with salmonella enterica serovar enteritidis, s. enterica serovar typhimurium, s. enterica serovar hadar, or s. enterica serovar infantis, respectivel ... | 2007 | 17709416 |
| contribution of the stg fimbrial operon of salmonella enterica serovar typhi during interaction with human cells. | salmonella serovars contain a wide variety of putative fimbrial systems that may contribute to colonization of specific niches. salmonella enterica serovar typhi is the etiologic agent of typhoid fever and is a pathogen specific to humans. in a previous study, we identified a gene, sty3920 (stgc), encoding the predicted usher of the stg fimbrial operon, that was expressed by serovar typhi during infection of human macrophages. the stg genes are located in the glms-psts intergenic region in serov ... | 2007 | 17709421 |
| omptin proteins: an expanding family of outer membrane proteases in gram-negative enterobacteriaceae. | the escherichia coli k-12 outer membrane protein ompt is a prototype of a unique family of bacterial endopeptidases known as the omptins. this family includes ompt and ompp of e. coli, sopa of shigella flexneri, pgte of salmonella enterica, and pla of yersinia pestis. despite their sequence similarities, the omptins vary in their reported functions. the ompt protease is characterized by narrow cleavage specificity defined by the extracellular loops of the beta-barrel protruding above the lipid b ... | 2007 | 17710644 |
| contribution of spi-4 genes to the virulence of salmonella enterica. | salmonella pathogenicity island-4 (spi-4) is a 27-kb region that carries six genes designated siiabcdef. siic, siid, and siif form a type i secretion apparatus for the secretion of siie, a huge (approximately 600 kda) protein contributing to the colonization of the bovine intestines. here it is shown that loss of spi-4 attenuates the oral virulence of salmonella enterica serovars typhimurium and enteritidis in mice. fifty percent lethal doses were elevated in both serovars upon the loss of spi-4 ... | 2007 | 17711458 |
| late recurrent salmonella sacroiliac osteomyelitis with psoas abscess in a non-sickle cell adult: case report. | 2007 | 17712436 | |
| predictive models for reduction of salmonella hadar on chicken skin during single and double sequential spraying treatments with acetic acid. | the response surface methodology was used to evaluate the effect of acetic acid concentration, spraying time and temperature on the reduction of salmonella hadar on poultry skin in a laboratory spraying process, and to identify the best conditions required to develop this operation. | 2007 | 17714385 |
| multiple-locus variable-number tandem repeat analysis of salmonella enterica subsp. enterica serovar typhimurium: comparison of isolates from pigs, poultry and cases of human gastroenteritis. | pulsed-field gel electrophoresis (pfge) and variable number tandem repeat (vntr) profiles of 195 epidemiologically unrelated salmonella typhimurium strains isolated in 1997-2004 from pigs were analysed and the results compared to establish the discriminatory ability of each method. in order to investigate the epidemiology of s. typhimurium from different populations, the vntr profiles from pigs were compared with those obtained from 190 s. typhimurium strains isolated from poultry and 186 strain ... | 2007 | 17714389 |
| growth inhibitory factors in bovine faeces impairs detection of salmonella dublin by conventional culture procedure. | to analyse the relative importance of different biological and technical factors on the analytical sensitivity of conventional culture methods for detection of salmonella dublin in cattle faeces. | 2007 | 17714398 |
| the lactobacillus plantarum strain aca-dc287 isolated from a greek cheese demonstrates antagonistic activity in vitro and in vivo against salmonella enterica serovar typhimurium. | the purpose of this study was to investigate the antibacterial activity of the xynotyri cheese isolate lactobacillus plantarum aca-dc287 using a set of in vitro and in vivo assays. | 2007 | 17714399 |
| identification and characterization of starter lactic acid bacteria and probiotics from columbian dairy products. | considering the significant rise in the probiotic market in columbia, and given the lack of reports concerning the microbial population and strain performance in products from different producers, this study aims at determining the number of viable starter bacteria and probiotics in bio-yoghurts available at the columbian market, identifying the species and analysing the performance of the isolated strains in bile acid resistance, antagonistic activity against pathogens, and adherence capacity t ... | 2007 | 17714400 |
| pyroptosis and host cell death responses during salmonella infection. | salmonella enterica are facultatively intracellular pathogens causing diseases with markedly visible signs of inflammation. during infection, salmonella interacts with various host cell types, often resulting in death of those cells. salmonella induces intestinal epithelial cell death via apoptosis, a cell death programme with a notably non-inflammatory outcome. in contrast, macrophage infection triggers caspase-1-dependent proinflammatory programmed cell death, a recently recognized process ter ... | 2007 | 17714514 |
| it's not easy being green. | 2007 | 17714671 | |
| salmonella pathogenicity islands in host specificity, host pathogen-interactions and antibiotics resistance of salmonella enterica. | salmonella enterica is a pathogen highly successful in causing a variety of gastrointestinal and systemic diseases in animals and humans. while some serovars of s. enterica are able to infect a broad range of host organisms, other serovars are highly restricted to specific host species. the colonization of hosts by s. enterica depends on the function of a large number of virulence determinants. the molecular analyses of virulence genes demonstrated that most of these loci are clustered within sa ... | 2007 | 17715824 |
| one step engineering of t7-expression strains for protein production: increasing the host-range of the t7-expression system. | the t7-expression system has been very useful for protein expression in escherichia coli. however, it is often desirable to over-express proteins in species other than e. coli. here, we constructed an inducible broad-host-range t7-expression transposon, which allows simple one-step construction of t7-expression strains in various species, providing the option to over-express proteins of interest in a broader host-range. this transposon contains the t7 rna polymerase driven by the lacuv5 promoter ... | 2007 | 17716915 |
| temporal fragmentation of speciation in bacteria. | because bacterial recombination involves the occasional transfer of small dna fragments between strains, different sets of niche-specific genes may be maintained in populations that freely recombine at other loci. therefore, genetic isolation may be established at different times for different chromosomal regions during speciation as recombination at niche-specific genes is curtailed. to test this model, we separated sequence divergence into rate and time components, revealing that different reg ... | 2007 | 17717188 |
| the pattern of pleiomorphism in stressed salmonella virchow populations is nutrient and growth phase dependent. | to describe the interactions of imposed osmotic and nutritional stress on the morphology of stationary and exponential phase s. virchow cells. | 2007 | 17718839 |
| bringing order to a complex molecular machine: the assembly of the bacterial flagella. | the bacterial flagellum is an example of elegance in molecular engineering. flagella dependent motility is a widespread and evolutionarily ancient trait. diverse bacterial species have evolved unique structural adaptations enabling them to migrate in their environmental niche. variability exists in the number, location and configuration of flagella, and reflects unique adaptations of the microorganism. the most detailed analysis of flagellar morphogenesis and structure has focused on escherichia ... | 2008 | 17719558 |
| protective action of lactobacillus kefir carrying s-layer protein against salmonella enterica serovar enteritidis. | eight lactobacillus kefir strains isolated from different kefir grains were tested for their ability to antagonize salmonella enterica serovar enteritidis (salmonella enteritidis) interaction with epithelial cells. l. kefir surface properties such as autoaggregation and coaggregation with salmonella and adhesion to caco-2/tc-7 cells were evaluated. l. kefir strains showed significantly different adhesion capacities, six strains were able to autoaggregate and four strains coaggregated with salmon ... | 2007 | 17719671 |
| monocyte and dendritic cell recruitment and activation during oral salmonella infection. | immunity to bacterial infection involves the joint effort of the innate and adaptive immune systems. the innate immune response is triggered when the body senses bacterial components, such as lipopolysaccharide, that alarm the body of the invader. an array of cell types function in the innate response. these cells are rapidly recruited to the infection site and activated to optimally perform their functions. the adaptive immune response follows the innate response, and one cell type in particula ... | 2007 | 17720254 |
| spontaneous zygogenesis, a wide-ranging mating process in bacteria. | spontaneous zygogenesis (or z-mating) in escherichia coli, unlike f-mediated conjugation, promotes formation of complete zygotes. most z-mating products have proven to be phenotypically unstable, losing part of the phenotype for which they were selected. persistent z-mating products appear as stable genetic recombinants or yield subclones of two types: either both parental types or one of them plus a recombinant type, and the ability to promote spontaneous zygogenesis can appear in all emerging ... | 2007 | 17720456 |
| cobalt targets multiple metabolic processes in salmonella enterica. | cobalt is essential for growth of salmonella enterica and other organisms, yet this metal can be toxic when present in excess. wild-type salmonella exhibits several metabolic defects when grown in the presence of cobalt, some of which generate visible growth consequences. work herein identifies sulfur assimilation, iron homeostasis, and fe-s cluster metabolism as targets for cobalt toxicity. in each case it is proposed that cobalt exerts its effect by one of two mechanisms: direct competition wi ... | 2007 | 17720790 |
| systemic translocation of salmonella enterica serovar dublin in cattle occurs predominantly via efferent lymphatics in a cell-free niche and requires type iii secretion system 1 (t3ss-1) but not t3ss-2. | salmonella enterica is an important diarrheal pathogen, and infections may involve severe systemic sequelae depending on serovar- and host-specific factors. the molecular mechanisms underlying translocation of host-restricted and -specific serovars of s. enterica from the intestines to distal organs are ill defined. by surgical cannulation of lymph and blood vessels draining the distal ileum in cattle, s. enterica serovar dublin was observed to translocate predominantly via mesenteric lymph node ... | 2007 | 17724072 |
| evaluation of phop and rpos mutants of salmonella enterica serovar typhi as attenuated typhoid vaccine candidates: virulence and protective immune responses in intranasally immunized mice. | the attenuation and immunoenhancing effects of rpos and phop salmonella enterica serovar strain typhi (salmonella typhi) mutants have not been compared. here, three s. typhi deletion mutants (phop, rpos, and rpos-phop double mutant) are constructed and these mutants are characterized with respect to invasiveness, virulence, and protective immune response compared with wild-type ty2. it was found that phop and phop-rpos deletion mutants are less invasive to ht-29 cells than the wild-type ty2 and ... | 2007 | 17725620 |
| the salmonella enterica serotype typhi regulator tvia reduces interleukin-8 production in intestinal epithelial cells by repressing flagellin secretion. | unlike non-typhoidal salmonella serotypes, s. enterica serotype typhi does not elicit neutrophilic infiltrates in the human intestinal mucosa. the vi capsule-encoding tviabcdevexabcde operon (viab locus) is a s. typhi-specific dna region preventing production of interleukin (il)-8 during infection of intestinal epithelial cells. we elucidated the mechanism by which the viab locus reduces il-8 production in human colonic epithelial (t84) cells. a s. typhi tviabcdevexabcde deletion mutant, but not ... | 2008 | 17725646 |
| a role for natural killer cells in intestinal inflammation caused by infection with salmonella enterica serovar typhimurium. | acute gastroenteritis caused by salmonella infection is a significant public health problem. using a mouse model of this condition, the authors demonstrated previously that the cytokine gamma interferon (ifn-gamma) is required for a normal intestinal inflammatory response to the pathogen. in the present study, these experiments are extended to show that natural killer (nk) cells constitute an early source of intestinal ifn-gamma during salmonella infection, and that these cells have a significan ... | 2007 | 17727655 |
| a new concept to stimulate mucosal as well as systemic immunity by parenteral vaccination as applied to the development of a live attenuated salmonella enterica serovar dublin vaccine. | a new concept of slow "drip feeding" that enables activation of mucosal as well as systemic immunity following parenteral vaccination was demonstrated using salmonella dublin in a mouse model. the live vaccine candidate, n-rm25, generated from a wild s. dublin strain utilising metabolic-drift (spontaneous chromosomal) mutations had a unique sensitivity to bile and restricted growth in the presence of a very low concentration of bile salts no. 3 (0.075% (w/v)) but also had the ability to survive ... | 2007 | 17727804 |
| pulmonary colonization with salmonella enterica serovar kentucky in an intensive care unit. | 2007 | 17728015 |