Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| genital ulcers: it is not always simplex …. | patients with vulval aphthae, also termed lipschütz ulcers, often present to genitourinary medicine clinics. typically, these ulcers present as acute, painful, vulval ulcers in young women and adolescents. the aetiology is unknown, and often these ulcers are accompanied by flu-like symptoms. previous case reports have linked such lesions to acute viral infections such as epstein-barr virus, cytomegalovirus and influenza a. we report the first case of influenza b virus and adenovirus infections a ... | 2015 | 24970475 |
| multicenter clinical evaluation of the novel alere™ i influenza a&b isothermal nucleic acid amplification test. | rapid detection of influenza infection is important for patient management and timely anti-viral therapy. rapid antigen detection tests for influenza have inferior sensitivity when compared to nucleic acid-based amplification tests. an isothermal nucleic acid amplification test that offers the potential for rapid molecular testing at the clinical point-of-care with simple equipment can improve influenza detection rates. | 2014 | 24973813 |
| risk factors for hospitalization among children with influenza b infection. | data regarding risk factors for hospitalization among children with influenza b infection are limited. we conducted a retrospective study of 184 children with influenza b infection during october 2011 - september 2012 seen at bamrasnaradura infectious diseases institute, thailand; clinical and laboratory data were compared between hospitalized and outpatient children. the numbers of hospitalized and outpatient children were 65 (35%) and 119 (65%), respectively. most children (> 80%) were aged > ... | 2014 | 24974646 |
| estimates of mortality attributable to influenza and rsv in the united states during 1997-2009 by influenza type or subtype, age, cause of death, and risk status. | influenza and respiratory syncytial virus (rsv) cause substantial mortality from respiratory and other causes in the usa, especially among people aged 65 and older. | 2014 | 24975705 |
| protective immunity based on the conserved hemagglutinin stalk domain and its prospects for universal influenza vaccine development. | influenza virus surface glycoprotein hemagglutinin (ha) is an excellent and chief target that elicits neutralizing antibodies during vaccination or natural infection. its ha2 subunit (stem domain) is most conserved as compared to ha1 subunit (globular head domain). current influenza vaccine relies on globular head domain that provides protection only against the homologous vaccine strains, rarely provides cross-protection against divergent strains, and needs to be updated annually. there is an u ... | 2014 | 24982895 |
| pathogenic influenza b virus in the ferret model establishes lower respiratory tract infection. | influenza b viruses have become increasingly more prominent during influenza seasons. influenza b infection is typically considered a mild disease and receives less attention than influenza a, but has been causing 20 to 50 % of the total influenza incidence in several regions around the world. although there is increasing evidence of mid to lower respiratory tract diseases such as bronchitis and pneumonia in influenza b patients, little is known about the pathogenesis of recent influenza b virus ... | 2014 | 24989173 |
| evaluation of alere i influenza a&b for rapid detection of influenza viruses a and b. | rapid and accurate diagnosis of influenza is important for infection control, as well as for patient management. alere i influenza a&b is an isothermal nucleic acid amplification-based integrated system for detection and differentiation of influenza virus a and influenza virus b. the performance of the alere i influenza a&b was screened using frozen nasopharyngeal-swab specimens collected in viral transport medium (vtm) that were originally tested fresh with the filmarray respiratory panel (rp) ... | 2014 | 24989611 |
| a flow-through chromatography process for influenza a and b virus purification. | vaccination is still the most efficient measure to protect against influenza virus infections. besides the seasonal wave of influenza, pandemic outbreaks of bird or swine flu represent a high threat to human population. with the establishment of cell culture-based processes, there is a growing demand for robust, economic and efficient downstream processes for influenza virus purification. this study focused on the development of an economic flow-through chromatographic process avoiding virus str ... | 2014 | 24992667 |
| 4flu - an individual based simulation tool to study the effects of quadrivalent vaccination on seasonal influenza in germany. | influenza vaccines contain influenza a and b antigens and are adjusted annually to match the characteristics of circulating viruses. in germany, influenza b viruses belonged to the b/yamagata lineage, but since 2001, the antigenically distinct b/victoria lineage has been co-circulating. trivalent influenza vaccines (tiv) contain antigens of the two a subtypes a(h3n2) and a(h1n1), yet of only one b lineage, resulting in frequent vaccine mismatches. since 2012, the who has been recommending vaccin ... | 2014 | 24993051 |
| viral etiology and seasonality of influenza-like illness in gabon, march 2010 to june 2011. | surveillance of influenza-like illness (ili) in central africa began only recently, and few data are therefore available on the circulation of influenza virus and other respiratory viruses. in gabon, a central african country, we established a surveillance network in four major towns in order to analyze cases of ili among patients who visited health centers between march 2010 and june 2011, and to determine the viral etiology. | 2014 | 25000832 |
| influenza a and b virus intertypic reassortment through compatible viral packaging signals. | influenza a and b viruses cocirculate in humans and together cause disease and seasonal epidemics. these two types of influenza viruses are evolutionarily divergent, and exchange of genetic segments inside coinfected cells occurs frequently within types but never between influenza a and b viruses. possible mechanisms inhibiting the intertypic reassortment of genetic segments could be due to incompatible protein functions of segment homologs, a lack of processing of heterotypic segments by influe ... | 2014 | 25008914 |
| etiology and clinical outcomes of acute respiratory virus infection in hospitalized adults. | etiologies and clinical profiles of acute respiratory viral infections need to be clarified to improve preventive and therapeutic strategies. | 2014 | 25024868 |
| effectiveness of trivalent seasonal influenza vaccine in preventing laboratory-confirmed influenza in primary care in the united kingdom: 2012/13 end of season results. | the effectiveness of the 2012/13 trivalent seasonal influenza vaccine (tiv) was assessed using a test-negative case-control study of patients consulting primary care with influenza-like illness in the united kingdom. strain characterisation was undertaken on selected isolates. vaccine effectiveness (ve) against confirmed influenza a(h3n2), a(h1n1) and b virus infection, adjusted for age, sex, surveillance scheme (i.e. setting) and month of sample collection was 26% (95% confidence interval (ci): ... | 2014 | 25033051 |
| a comparison of the demographic and clinical characteristics of laboratory-confirmed influenza b yamagata and victoria lineage infection. | the evolution of influenza viruses is characterized by the co-circulation of two antigenically and genetically distinct lineages b/victoria/2/87-like viruses (victoria) and b/yamagata/16/88-like viruses (yamagata). to date, there is not much data associating lineages variation with demographic and clinical data. | 2014 | 25034374 |
| epidemiology and impact of influenza in mongolia, 2007-2012. | mongolia's health service began to conduct surveillance for influenza in the 1970s. this surveillance has become more comprehensive over time and now includes 155 sentinel sites in mongolia. in this study, we analyzed the epidemiological characteristics and impact of influenza using data from influenza surveillance in mongolia. | 2014 | 25043147 |
| influenza vaccine effectiveness in australia: results from the australian sentinel practices research network. | to estimate influenza vaccine coverage and effectiveness against medically attended laboratory-confirmed influenza for the 2012 season. | 2014 | 25045991 |
| live attenuated influenza vaccine. | cold-adapted ann arbor based live attenuated influenza vaccine (laiv) has been available in the usa since 2003. the vaccine is efficacious against influenza infection. features of laiv include: easy administration suitable for mass immunization, cross-reactivity to drifted strains for broader coverage, and establishment of herd immunity for control of influenza spread. annual seasonal laiv now contains four strains against influenza a h1n1, h3n2, influenza b-victoria, and b-yamagata lineages tha ... | 2015 | 25059893 |
| sentinel surveillance of influenza in burkina faso: identification of circulating strains during 2010-2012. | although influenza surveillance has recently been improved in some sub-saharan african countries, no information is yet available from burkina faso. | 2014 | 25074591 |
| detection of varying influenza circulation within england in 2012/13: informing antiviral prescription and public health response. | subnational variation of 2009 pandemic influenza activity in england has been reported; however, little work has been published on this topic for seasonal influenza. if variation is present, this knowledge may assist with both identifying the onset of influenza epidemics, informing community antiviral prescription and local health planning. | 2015 | 25096307 |
| influenza-like illness sentinel surveillance in one hospital in medellin, colombia. 2007-2012. | the city of medellin in colombia has almost no documentation of the causes of acute respiratory infections (aris). as part of an ongoing collaboration, we conducted an epidemiologic surveillance for influenza and other respiratory viruses. it described the influenza strains that were circulating in the region along with their distribution over time, and performing molecular characterization to some of those strains. this will contribute to the knowledge of local and national epidemiology. | 2015 | 25100179 |
| comparison of severe pneumonia caused by human metapneumovirus and respiratory syncytial virus in hospitalized children. | the objective of this study is to compare the incidence and clinical characteristics of severe pneumonia caused by human metapneumovirus (hmpv) to respiratory syncytial virus (rsv) infection in children. | 2014 | 25118733 |
| the n terminus of the influenza b virus nucleoprotein is essential for virus viability, nuclear localization, and optimal transcription and replication of the viral genome. | the nucleoprotein (np) of influenza viruses is a multifunctional protein with essential roles throughout viral replication. despite influenza a and b viruses belonging to separate genera of the orthomyxoviridae family, their np proteins share a relatively high level of sequence conservation. however, np of influenza b viruses (bnp) contains an evolutionarily conserved n-terminal 50-amino-acid extension that is absent from np of influenza a viruses. there is conflicting evidence as to the functio ... | 2014 | 25122787 |
| tropical influenza and weather variability among children in an urban low-income population in bangladesh. | influenza seasonality in the tropics is poorly understood and not as well documented as in temperate regions. in addition, low-income populations are considered highly vulnerable to such acute respiratory disease, owing to limited resources and overcrowding. nonetheless, little is known about their actual disease burden for lack of data. we therefore investigated associations between tropical influenza incidence and weather variability among children under five in a poor urban area of dhaka, ban ... | 2014 | 25128806 |
| editorial commentary: changing epidemiology of influenza b virus. | 2014 | 25139967 | |
| impact of influenza b lineage-level mismatch between trivalent seasonal influenza vaccines and circulating viruses, 1999-2012. | influenza b virus strains in trivalent influenza vaccines are frequently mismatched to the circulating b strains, but the population-level impact of such mismatches is unknown. we assessed the impact of vaccine mismatch on the epidemiology of influenza b during 12 recent seasonal outbreaks of influenza in finland. | 2014 | 25139969 |
| respiratory virus is a real pathogen in immunocompetent community-acquired pneumonia: comparing to influenza like illness and volunteer controls. | viral pathogens were more commonly reported than previously estimated in community-acquired pneumonia (cap) patients. however, the real role of virus was still controversial. | 2014 | 25178477 |
| comparison of three commercial rt-pcr systems for the detection of respiratory viruses. | due to the insensitivity of rapid tests for respiratory viruses, nucleic acid amplification tests are quickly becoming the standard of care. | 2014 | 25183359 |
| performance of the simplexa™ flu a/b & rsv direct kit on respiratory samples collected in saline solution. | molecular assays for diagnosis of influenza a, influenza b, and respiratory syncytial virus (rsv) with short turnaround time are of considerable clinical importance. we have evaluated the diagnostic performance of the simplexa(™) flu a/b & rsv direct kit, which has a run time of 60 min, using different types of respiratory samples collected from patients with a suspected respiratory tract infection, including materials not previously evaluated on this kit. | 2014 | 25195649 |
| using daily syndrome-specific absence data for early detection of school outbreaks: a pilot study in rural china. | this paper describes and preliminarily evaluates the usefulness of the daily syndrome-specific absenteeism surveillance system (dssass) as an early warning system of school outbreaks in rural china. | 2014 | 25200208 |
| assessment of the antiviral properties of recombinant surfactant protein d against influenza b virus in vitro. | the armamentarium of antiviral drugs against influenza viruses is limited. furthermore, influenza viruses emerge that are resistant to existing antiviral drugs like the m2 and na inhibitors. therefore, there is an urgent need for the development of novel classes of antiviral drugs. here we investigated the antiviral properties of recombinant porcine surfactant protein d (rpsp-d), an innate defense molecule with lectin properties, against influenza b viruses. we have previously shown that porcine ... | 2015 | 25200748 |
| comparison of the simplexa™ flu a/b & rsv kit (nucleic acid extraction-dependent assay) and the prodessa proflu+™ assay for detecting influenza and respiratory syncytial viruses. | the relative performance of 2 widely used reverse transcription polymerase chain reaction (rt-pcr) assays, the focus diagnostics simplexa™ flu a/b & rsv kit (nucleic acid extraction-dependent assay) and the prodessa proflu+™ assay, was evaluated using 735 prospectively and retrospectively collected nasopharyngeal swab specimens. overall, the assays showed positive and negative agreements of 100% and 99.7% for influenza a, 98.1% and 99.9% for influenza b, and 99.3% and 99.5% for respiratory syncy ... | 2014 | 25209363 |
| influenza vaccines to control influenza-associated bacterial infection: where do we stand? | influenza a virus is a pathogen that is feared for its capacity to cause pandemics. in this review, we illustrate the clinical evidence which support the theory that bacterial co-infection is a considerable risk factor for exacerbated disease during pandemic and seasonal influenza, including infection with influenza b viruses. we provide an overview of the multiple and diverse mechanisms that help explain how influenza creates an opportunity for replication of secondary bacterial infections. inf ... | 2015 | 25209381 |
| evaluation of the alere i influenza a&b nucleic acid amplification test by use of respiratory specimens collected in viral transport medium. | the alere i influenza a&b assay is a newly developed rapid molecular assay which has the potential to generate results within 15 min from sample collection. in this study, we evaluated the alere i influenza a&b assay by using salvaged frozen respiratory specimens that were collected in viral transport medium from children ages 10 months to 19 years. alere i influenza a&b assay test results were compared with viral culture and proflu(+) real-time reverse transcription-pcr (rt-pcr) assay results. ... | 2014 | 25210070 |
| effectiveness of post-exposition prophylaxis with oseltamivir in nursing homes: a randomised controlled trial over four seasons. | oseltamivir has been registered for use as post-exposition prophylaxis (pep) following exposure to influenza, based on studies among healthy adults. effectiveness among frail elderly nursing home populations still needs to be properly assessed. | 2014 | 25210532 |
| influenza epidemiology, vaccine coverage and vaccine effectiveness in sentinel australian hospitals in 2013: the influenza complications alert network. | the national influenza program aims to reduce serious morbidity and mortality from influenza by providing public funding for vaccination to at-risk groups. the influenza complications alert network (flucan) is a sentinel hospital-based surveillance program that operates at 14 sites in all states and territories in australia. this report summarises the epidemiology of hospitalisations with confirmed influenza, estimates vaccine coverage and influenza vaccine protection against hospitalisation wit ... | 2014 | 25222208 |
| influenza vaccine effectiveness estimates for western australia during a period of vaccine and virus strain stability, 2010 to 2012. | during 2010-2012 the strain composition of the influenza vaccine in the southern hemisphere did not change, but the circulating virus type/subtype did. we pooled data for these years from the western australian sentinel medical practice surveillance system for influenza to estimate vaccine effectiveness (ve) by influenza virus type and subtype. a case test-negative design was used with ve estimated as (1-odds ratio)×100%. there were 2182 patients included in the analysis across the 3 years studi ... | 2014 | 25223268 |
| influenza virus infection of marine mammals. | interspecies transmission may play a key role in the evolution and ecology of influenza a viruses. the importance of marine mammals as hosts or carriers of potential zoonotic pathogens such as highly pathogenic h5 and h7 influenza viruses is not well understood. the fact that influenza viruses are some of the few zoonotic pathogens known to have caused infection in marine mammals, evidence for direct transmission of influenza a virus h7n7 subtype from seals to man, transmission of pandemic h1n1 ... | 2016 | 25231137 |
| implementation of influenza-like illness sentinel surveillance in togo. | the emergence of avian influenza a/h5n1 in 2003 as well as the pandemic influenza a (h1n1) pdm09 highlighted the need to establish influenza sentinel surveillance in togo. the ministry of health decided to introduce influenza to the list of diseases with epidemic potential. by april 2010, togo was actively involved in influenza surveillance. this study aims to describe the implementation of ili surveillance and results obtained from april 2010 to december 2012. | 2014 | 25239536 |
| evaluation of 3 rapid influenza diagnostic tests during the 2012-2013 epidemic: influences of subtype and viral load. | this article evaluates the performance of 3 rapid influenza diagnostic tests (ridts), in correlation with the influenza subtypes and the viral load. a total of 236 samples were prospectively analyzed with binaxnow influenza a/b, directigen ez flu a and b, and bionexia influenza a+b. the results were compared to cell cultures and real-time polymerase chain reaction. positive samples were further subtyped. thirty-seven samples were positive for influenza a, and 57, for influenza b. for a(h1n1), th ... | 2014 | 25241638 |
| evaluation of a rapid antigen detection point-of-care test for respiratory syncytial virus and influenza in a pediatric hospitalized population in the netherlands. | this pilot study evaluates the diagnostic performance of sofia rsv fluorescent immunoassay analyzer (fia) and sofia influenza a + b fia for rapid detection of respiratory syncytial virus and influenza a and b. sofia had a lower-than-expected sensitivity for all viruses and a high rate of false-positive results for influenza b virus. | 2014 | 25241640 |
| [severe upper abdominal pain during a long distance flight]. | a 43-year-old woman of ghanaian origin presented with severe upper abdominal pain starting on a long distance flight. physical examination revealed tenderness on palpation in the left upper abdomen and flank. there was no report of pre-existing conditions or permanent medication in the medical history. | 2014 | 25254402 |
| burden of influenza b virus infections in scotland in 2012/13 and epidemiological investigations between 2000 and 2012. | 2014 | 25259532 | |
| modes of transmission of influenza b virus in households. | while influenza a and b viruses can be transmitted via respiratory droplets, the importance of small droplet nuclei "aerosols" in transmission is controversial. | 2014 | 25268241 |
| [detection of respiratory viruses in influenza-like illness in shijiazhuang, china in 2011]. | this study aimed to investigate viral infections and the prevalence of influenza-like illness (ili) in shijiazhuang, china, in 2011 and to provide a scientific basis for the diagnosis and control of respiratory tract infections. throat swab specimens were collected from 483 cases of ili who were outpatients in the influenza surveillance sentinel hospitals in shijiazhuang between january and december 2011. all specimens were examined by multiplex rt-pcr for the following 15 respiratory tract viru ... | 2014 | 25272592 |
| comparison of the imdx influenza a virus, influenza b virus, and respiratory syncytial virus a/b assay on the m2000 platform with real-time reverse transcriptase pcr assays. | 2014 | 25275003 | |
| update: influenza activity -- united states and worldwide, may 18-september 20, 2014. | during may 18-september 20, 2014, the united states experienced low levels of seasonal influenza activity overall. influenza a (h1n1)pdm09 (ph1n1), influenza a (h3n2), and influenza b viruses were detected worldwide and were identified sporadically in the united states. in august, two influenza a (h3n2) variant viruses (h3n2v) were detected in ohio. this report summarizes influenza activity in the united states and worldwide during may 18-september 20, 2014. | 2014 | 25275330 |
| in vitro neuraminidase inhibitory activity of four neuraminidase inhibitors against clinical isolates of influenza virus in the japanese 2012-2013 season. | the neuraminidase inhibitors (nais) oseltamivir phosphate (tamiflu(®)), zanamivir (relenza(®)), laninamivir octanoate (inavir(®)), and peramivir (rapiacta(®)) have been available for the treatment of influenza in japan since 2010. the emergence of resistant virus to any of the nais is a great concern for influenza treatment. to assess the extent of viral resistance, we measured the 50% inhibitory concentration (ic50) of each nai for influenza virus isolates in the 2012-2013 influenza season and ... | 2015 | 25277670 |
| relative timing of influenza disease by age group. | a detailed understanding of influenza movement in communities during yearly epidemics is needed to inform improved influenza control programs. we sought to determine the relative timing of influenza presentation and symptom onset by age group and influenza strain. prospective, laboratory-confirmed surveillance was performed over three moderate influenza seasons in emergency departments and inpatient settings of both medical centers in winston-salem, nc. influenza disease presented first in schoo ... | 2014 | 25280434 |
| interactions of lipids and detergents with a viral ion channel protein: molecular dynamics simulation studies. | structural studies of membrane proteins have highlighted the likely influence of membrane mimetic environments (i.e., lipid bilayers versus detergent micelles) on the conformation and dynamics of small α-helical membrane proteins. we have used molecular dynamics simulations to compare the conformational dynamics of bm2 (a small α-helical protein from the membrane of influenza b) in a model phospholipid bilayer environment with its behavior in protein-detergent complexes with either the zwitterio ... | 2015 | 25286030 |
| a highly sensitive europium nanoparticle-based immunoassay for detection of influenza a/b virus antigen in clinical specimens. | we report the development of a novel europium nanoparticle-based immunoassay (enia) for rapid detection of influenza a and influenza b viruses. the enia demonstrated sensitivities of 90.7% (147/162) for influenza a viruses and 81.80% (9/11) for influenza b viruses compared to those for an in-house reverse transcription (rt)-pcr assay in testing of influenza-positive clinical samples. | 2014 | 25297327 |
| influenza season in réunion dominated by influenza b virus circulation associated with numerous cases of severe disease, france, 2014. | the 2014 seasonal influenza in réunion, a french overseas territory in the southern hemisphere, was dominated by influenza b. resulting morbidity impacted public health. relative to the total number of all-cause consultations over the whole season, the rate of acute respiratory infection (ari) consultations was 6.5%. severe disease occurred in 32 laboratory-confirmed influenza cases (31.7 per 100,000 ari consultations), 16 with influenza b. the observed disease dynamics could present a potential ... | 2014 | 25306979 |
| optic neuritis associated with influenza b virus meningoencephalitis. | various postinfectious neurological manifestations have been described associated to influenza viruses. optic neuritis is a serious, often reversible disease reported among several infectious diseases and vaccines complications. we report a case of optic neuritis following an influenza b virus infection in a 10-year-old male. | 2014 | 25308101 |
| fatal clinical course of probable invasive pulmonary aspergillosis with influenza b infection in an immunocompetent patient. | invasive pulmonary aspergillosis (ipa) is rarely reported in patients who have normal immune function. recently, ipa risk was reported in nonimmunocompromised hosts, such as patients with chronic obstructive pulmonary disease and critically ill patients in intensive care units. moreover, influenza infection is also believed to be associated with ipa among immunocompetent patients. however, most reports on ipa with influenza a infection, including pandemic influenza h1n1, and ipa associated with ... | 2014 | 25309610 |
| canine susceptibility to human influenza viruses (a/pdm 09h1n1, a/h3n2 and b). | we investigated the infectivity and transmissibility of the human seasonal h3n2, pandemic (pdm) h1n1 (2009) and b influenza viruses in dogs. dogs inoculated with human seasonal h3n2 and pdm h1n1 influenza viruses exhibited nasal shedding and were seroconverted against the viruses; this did not occur in the influenza b virus-inoculated dogs. transmission of human h3n2 virus between dogs was demonstrated by observing nasal shedding and seroconversion in naïve dogs after contact with inoculated dog ... | 2015 | 25312584 |
| risk factors of prolonged hospital stay in children with viral severe acute respiratory infections. | severe acute lower respiratory infections (saris) are one of the major causes of morbidity and mortality in young children, especially in developing countries. the present study focused on detection of risk factors for prolonged hospital stays among children with viral saris. | 2014 | 25313605 |
| novel mode of isg15-mediated protection against influenza a virus and sendai virus in mice. | isg15 is a diubiquitin-like modifier and one of the most rapidly induced genes upon type i interferon stimulation. hundreds of host proteins and a number of viral proteins have been shown to be isgylated, and understanding how these modifications affect the interferon response and virus replication has been of considerable interest. isg15(-/-) mice exhibit increased susceptibility to viral infection, and in the case of influenza b virus and vaccinia virus, isg15 conjugation has been shown to res ... | 2014 | 25320315 |
| microarray multiplex assay for the simultaneous detection and discrimination of influenza a and influenza b viruses. | in this study, we present a microarray approach for the typing of influenza a and b viruses, and the subtyping of h1 and h3 subtypes. we designed four pairs of specific multiplex rt-pcr primers and eight specific oligonucleotide probes and prepared microarrays to identify the specific subtype of influenza virus. through amplification and fluorescent marking of the multiplex rt-pcr products on the m gene of influenza a and b viruses and the ha gene of subtypes h1 and h3, the pcr products were hyb ... | 2014 | 25320424 |
| reassortment between influenza b lineages and the emergence of a coadapted pb1-pb2-ha gene complex. | influenza b viruses make a considerable contribution to morbidity attributed to seasonal influenza. currently circulating influenza b isolates are known to belong to two antigenically distinct lineages referred to as b/victoria and b/yamagata. frequent exchange of genomic segments of these two lineages has been noted in the past, but the observed patterns of reassortment have not been formalized in detail. we investigate interlineage reassortments by comparing phylogenetic trees across genomic s ... | 2015 | 25323575 |
| epidemiological and virological characteristics of influenza viruses circulating in cambodia from 2009 to 2011. | the cambodian national influenza center (nic) monitored and characterized circulating influenza strains from 2009 to 2011. | 2014 | 25340711 |
| human rhinovirus c infections in pediatric hematology and oncology patients. | children with cancer and hsct recipients are at high risk for common viral infections. we sought to define the viral etiology of ari and identify risk factors. nasal wash samples were collected from pediatric hematology-oncology patients and hsct recipients with ari during the 2003-2005 winter seasons. real-time rt-pcr was performed to detect flu a, influenza b, rsv, piv 1-3, human mpv, and hrv. hrv specimens were sequenced and genotyped. seventy-eight samples from 62 children were included. vir ... | 2015 | 25377237 |
| case series of rash associated with influenza b in school children. | this case series describes morbilliform and other rash presentations among schoolchildren during a march 2014 outbreak of influenza-like illness (ili) in british columbia, canada. multiplex nucleic acid testing of nasopharyngeal specimens and paired serologic investigations identified that influenza b, characterized as b/massachusetts/02/2012-like (yamagata-lineage), was the only viral aetiology and most likely cause of ili and rash. an association between influenza b and rash has been described ... | 2015 | 25382064 |
| antibody and cell-mediated immune response to whole virion and split virion influenza vaccine in patients with inflammatory bowel disease on maintenance immunosuppressive and biological therapy. | influenza vaccination is recommended for inflammatory bowel disease (ibd) patients on immunosuppressive therapy. the objective was to evaluate the antibody and cell-mediated immune response to the split and whole virion influenza vaccine in patients with ibd treated with anti-tnf-α and immunosuppressive therapy. | 2015 | 25384624 |
| immunogenicity and safety of inactivated quadrivalent and trivalent influenza vaccines in children 18-47 months of age. | because inactivated trivalent influenza vaccines (tivs) contain 1 influenza b strain, whereas 2 lineages may co-circulate, b lineage mismatch is frequent. we assessed an inactivated quadrivalent influenza vaccine (qiv) containing both b lineages versus tiv in young children. | 2014 | 25386965 |
| risk factors and outcomes of influenza infection among children presenting with influenza-like illness. | limited data were available to guide management, counseling, and/or diagnostic investigation among children presenting with influenza-like illness (ili). during a recent period of high influenza activity, we wished to determine the frequency, outcomes, and factors associated with influenza infection among children presenting with ili. | 2014 | 25391171 |
| the enigma ml fluab-rsv assay: a fully automated molecular test for the rapid detection of influenza a, b and respiratory syncytial viruses in respiratory specimens. | the enigma(®) ml fluab-rsv assay (enigma diagnostics, porton down, salisbury, uk) is a ce-ivd marked multiplex molecular panel for the detection of influenza a, b and respiratory syncytial viruses in nasopharyngeal swabs. the assay runs on the fully automated enigma ml platform without further specimen manipulation and provides a sample-to-answer result within 95 min. the reported sensitivity and specificity for influenza a are 100% (95% ci: 98.2-100) and 98.3% (95% ci: 95.5-99.4), respectively, ... | 2015 | 25399865 |
| influenza vaccine effectiveness in the united states during 2012-2013: variable protection by age and virus type. | during the 2012-2013 influenza season, there was cocirculation of influenza a(h3n2) and 2 influenza b lineage viruses in the united states. | 2015 | 25406334 |
| structural insight into cap-snatching and rna synthesis by influenza polymerase. | influenza virus polymerase uses a capped primer, derived by 'cap-snatching' from host pre-messenger rna, to transcribe its rna genome into mrna and a stuttering mechanism to generate the poly(a) tail. by contrast, genome replication is unprimed and generates exact full-length copies of the template. here we use crystal structures of bat influenza a and human influenza b polymerases (flua and flub), bound to the viral rna promoter, to give mechanistic insight into these distinct processes. in the ... | 2014 | 25409151 |
| molecular markers of influenza b lineages and clades. | co-circulation of two influenza b virus lineages, b/yamagata and b/victoria, has been recognized since the late 1980s. the assessment of the prevalent lineage and the group of viruses in circulation is of importance in order to decide on the vaccine composition and evaluate its efficacy. the molecular characterization of influenza b viruses in circulation has been the aim of this study; this was approached by identifying and locating nucleotide substitutions in the influenza b virus hemagglutini ... | 2014 | 25412364 |
| clinical manifestations of influenza a and b in children and adults at a tertiary hospital in korea during the 2011-2012 season. | this study aims to evaluate and compare the epidemiological patterns and clinical courses of influenza a and b among children and adults. for this purpose, we retrospectively reviewed the medical records of 809 children and 271 adolescents/adults with laboratory-confirmed influenza between october 2011 and may 2012 at a tertiary-care hospital. children with influenza b presented with high fever (body temperature >39℃), sputum production, diarrhea, nausea/vomiting, and myalgia more frequently tha ... | 2015 | 25420662 |
| cost-effectiveness analysis of quadrivalent influenza vaccine versus trivalent influenza vaccine for elderly in hong kong. | cost and quality-adjusted life-years (qalys) gained by quadrivalent influenza vaccine (qiv) versus trivalent influenza vaccine (tiv) in hong kong elderly were estimated over 9 seasons. | 2014 | 25420713 |
| influenza pneumonia surveillance among hospitalized adults may underestimate the burden of severe influenza disease. | studies seeking to estimate the burden of influenza among hospitalized adults often use case definitions that require presence of pneumonia. the goal of this study was to assess the extent to which restricting influenza testing to adults hospitalized with pneumonia could underestimate the total burden of hospitalized influenza disease. | 2014 | 25423025 |
| genetic characterisation of influenza b viruses detected in singapore, 2004 to 2009. | influenza b viruses are classified into two main lineages: yamagata-like and victoria-like, which differ antigenically and phylogenetically. to understand the evolution of influenza b viruses in south east asia as well as to determine the vaccine efficacy, we genetically characterised gene segments 4, 6 and 8 from non-tissue culture adapted influenza b viruses detected in singapore from 2004 to 2009. | 2014 | 25435177 |
| descriptive epidemiology of school outbreaks of seasonal influenza b during 2012/2013 in the thames valley, united kingdom. | 2014 | 25443101 | |
| continued effectiveness of laninamivir octanate hydrate for influenza treatment in japan: comparison between the 2011-2012 and 2012-2013 influenza seasons. | the clinical effectiveness of laninamivir octanoate hydrate (laninamivir) was investigated in the japanese 2012-2013 influenza season for comparison with that of the japanese 2011-2012 influenza season. a total of 235 patients were enrolled, of whom 210 were evaluated for the duration of fever and other symptoms. the median durations of fever for a(h3n2) were 32.0 and 38.0 h and the median durations of symptoms for the a(h3n2) were 102.0 and 84.0 h for patients aged under 10 and 10 years or olde ... | 2014 | 25444677 |
| understanding influenza vaccine protection in the community: an assessment of the 2013 influenza season in victoria, australia. | the influenza virus undergoes frequent antigenic drift, necessitating annual review of the composition of the influenza vaccine. vaccination is an important strategy for reducing the impact and burden of influenza, and estimating vaccine effectiveness (ve) each year informs surveillance and preventative measures. we aimed to describe the influenza season and to estimate the effectiveness of the influenza vaccine in victoria, australia, in 2013. | 2015 | 25448093 |
| many respiratory viruses have temporal association with meningococcal disease. | previous ecological studies have shown a temporal and spatial association between influenza epidemics and meningococcal disease (mng); however, none have examined more than two respiratory viruses. | 2015 | 25456529 |
| environmental sampling for respiratory pathogens in jeddah airport during the 2013 hajj season. | respiratory tract infections (rtis) are common during the hajj season and are caused by a variety of organisms, which can be transmitted via the air or contaminated surfaces. we conducted a study aimed at sampling the environment in the king abdul aziz international (kaai) airport, pilgrims city, jeddah, during hajj season to detect respiratory pathogens. | 2014 | 25465254 |
| evaluation of safety and efficacy of intravenous zanamivir in the treatment of hospitalized japanese patients with influenza: an open-label, single-arm study. | zanamivir is a neuraminidase (na) inhibitor used for the treatment of influenza. there is an unmet need for a parenteral influenza antiviral therapy to treat hospitalized patients. therefore the safety and efficacy profile of intravenous zanamivir (ivz) was examined in japanese patients with severe influenza. | 2015 | 25470818 |
| influenza virus surveillance among young children in são paulo, brazil: the impact of vaccination. | this study assessed the presence of influenza virus among young children and the coverage of vaccination from 2010 to 2012 in são paulo, brazil. our results demonstrated a lower rate of influenza detection and a predominance of influenza b. a decrease of coverage vaccination through the surveillance periods was observed. | 2014 | 25477951 |
| computational insights into the inhibition of influenza viruses by rupestonic acid derivatives: pharmacophore modeling, 3d-qsar, comfa and comsia studies. | the pharmacophore modeling and 3d-qsar studies were performed on a series of amino alkyl rupestonates (rupestonic acid) derivatives reported for h1n1, h3n2 and influenza b virus, na inhibition. in order to improve the efficacy of amino alkyl rupestonates derivatives, a four point pharmacophore model with one acceptor and three hydrophobic regions was developed. furthermore, the 3d-qsar model was generated based on the pharmacophore hypothesis (ahhh) for each subtype. the hypothesis was more sign ... | 2015 | 25483013 |
| [simultaneous detection of respiratory viruses and influenza a virus subtypes using multiplex pcr]. | this study was conducted to investigate the respiratory viruses and subtyping of influenza a virus when positive by multiplex pcr in patients with flu-like symptoms, after the pandemic caused by influenza a (h1n1)pdm09. nasopharyngeal swab samples collected from 700 patients (313 female, 387 male; age range: 24 days-94 yrs, median age: 1 yr) between december 2010 - january 2013 with flu-like symptoms including fever, headache, sore throat, rhinitis, cough, myalgia as defined by the world health ... | 2014 | 25492660 |
| natural attack rate of influenza in unvaccinated children and adults: a meta-regression analysis. | the natural (i.e. unvaccinated population) attack rate of an infectious disease is an important parameter required for understanding disease transmission. as such, it is an input parameter in infectious disease mathematical models. influenza is an infectious disease that poses a major health concern worldwide and the natural attack rate of this disease is crucial in determining the effectiveness and cost-effectiveness of public health interventions and informing surveillance program design. we e ... | 2014 | 25495228 |
| neuraminidase mutations conferring resistance to laninamivir lead to faster drug binding and dissociation. | the neuraminidase (na) inhibitors oseltamivir and zanamivir are administered twice daily for 5days for treatment of influenza. laninamivir is a 7-methoxy derivative of zanamivir, but a single dose is effective when taken as the laninamivir octanoate prodrug. we show here in ic50 kinetics assays and a solid phase reactivation assay that compared to zanamivir laninamivir also demonstrates slow binding to but slower dissociation from multiple wild type nas. a d197e mutation in an influenza b and an ... | 2015 | 25499124 |
| performance of the molecular alere i influenza a&b test compared to that of the xpert flu a/b assay. | data on the performance of rapid molecular point-of-care use platforms for diagnosis of influenza are lacking. we validated nasopharyngeal (np) flocked specimens in universal transport medium (utm) and evaluated the clinical sensitivity and specificity of the alere i influenza a&b test compared to those of the xpert flu a/b assay. the alere i influenza a&b test had an overall sensitivity and specificity of 93.8% and 62.5% for influenza a, respectively, and of 91.8% and 53.6% for influenza b, res ... | 2015 | 25502527 |
| [the 2013-2014 epidemic season. hospital monitoring and antiviral therapy for influenza ]. | to characterize the 2013-2014 epidemic season from the results of detection of influenza infection in patients; to provide the molecular genetic characteristics of the strains isolated from deceased patients. | 2014 | 25509893 |
| differentiation of influenza b lineages from clinical samples by one-step real-time rt-pcr. | influenza viruses type a and type b are a leading cause of annual epidemics in human populations. since the 1970s, influenza b viruses have diverged into two antigenically distinct virus lineages called the yamagata and victoria lineages. we describe the validation and implementation of a one-step real-time rt-pcr (rrt-pcr) assay that can differentiate between the two genetic lineages of type b. | 2014 | 25518567 |
| randomized, controlled trial of high-dose influenza vaccine among frail residents of long-term care facilities. | despite vaccination, residents of long-term-care facilities (ltcfs) remain at high risk of influenza-related morbidity and mortality. more-effective vaccine options for this population are needed. | 2015 | 25525051 |
| development of high yield reassortants for influenza type b viruses and analysis of their gene compositions. | a critical step in producing the annual inactivated influenza vaccine is the development of high yield (hy) seed viruses by reassortment for improved growth in ovo. although hy reassortants for type a influenza viruses have been developed for many years, hy b influenza reassortant virus development for vaccine production has proven difficult. in this study, we have developed fourteen hy influenza type b reassortants as vaccine candidate strains with b/lee/40 as the donor virus. upon characteriza ... | 2015 | 25545595 |
| influenza viral load and peramivir kinetics after single administration and proposal of regimens for peramivir administration against resistant variants. | we estimated the efficacy of the current single administration of peramivir on the basis of peramivir pharmacokinetics in the upper respiratory tract (urt) and determined the predictive peramivir concentration-time curve to assess its efficacy against viruses with decreased susceptibility to neuraminidase inhibitors. serum, nasal swab, or aspiration samples were collected from 28 patients treated with 10 mg/kg body weight peramivir. the sequential influenza viral rna load and susceptibility afte ... | 2015 | 25547357 |
| need for an improved vaccination rate in primary immune thrombocytopenia patients exposed to rituximab or splenectomy. a nationwide population-based study in france. | international guidelines on immune thrombocytopenia (itp) management recommend vaccination against streptococcus pneumoniae (s.p.), haemophilus influenza b (hib) and neisseiria meningitidis (n.m.) before splenectomy. french guidelines also recommend these vaccinations before rituximab. the aim of this study was to assess the application of these recommendations. the french adult itp: a french pharmacoepidemiological study (faith, n°encepp 4574) is aimed at following in the french national health ... | 2015 | 25557586 |
| comparison of multiplex rt-pcr with virus isolation for detection, typing and sub-typing of influenza virus from influenza-like illness cases. | influenza epidemics and periodic pandemics occur worldwide resulting in significant mortality, morbidity and economic loss. there is need for a sensitive, rapid and cost-effective assay to detect, type and sub-type influenza viruses, as cell culture has a long turnaround time. | 2015 | 25560005 |
| ghrelin-reactive immunoglobulins and anxiety, depression and stress-induced cortisol response in adolescents. the trails study. | ghrelin, a hunger hormone, has been implicated in the regulation of stress-response, anxiety and depression. ghrelin-reactive immunoglobulins (ig) were recently identified in healthy and obese humans showing abilities to increase ghrelin's stability and orexigenic effects. here we studied if ghrelin-reactive ig are associated with anxiety and depression and with the stress-induced cortisol response in a general population of adolescents. furthermore, to test the possible infectious origin of ghr ... | 2015 | 25562566 |
| detection of influenza virus infection using two pcr methods. | rapid, accurate, and cost-effective methods to identify the cause of respiratory tract infections are needed to maximize clinical benefit. outpatients with acute respiratory illness were tested for influenza using a singleplex reverse transcriptase polymerase chain reaction (srt-pcr) method. a multiplex rt-pcr (mrt-pcr) method tested for influenza and 17 other viruses and was compared with srt-pcr using chi-square tests. among 935 patients, 335 (36%) tested positive for influenza a and influenza ... | 2014 | 25574169 |
| early estimates of seasonal influenza vaccine effectiveness - united states, january 2015. | in the united states, annual vaccination against seasonal influenza is recommended for all persons aged ≥6 months. each season since 2004-05, cdc has estimated the effectiveness of seasonal influenza vaccine in preventing medically attended acute respiratory illness (ari) associated with laboratory-confirmed influenza. this season, early estimates of influenza vaccine effectiveness are possible because of widespread, early circulation of influenza viruses. by january 3, 2015, 46 states were expe ... | 2015 | 25590680 |
| the contrasting phylodynamics of human influenza b viruses. | a complex interplay of viral, host, and ecological factors shapes the spatio-temporal incidence and evolution of human influenza viruses. although considerable attention has been paid to influenza a viruses, a lack of equivalent data means that an integrated evolutionary and epidemiological framework has until now not been available for influenza b viruses, despite their significant disease burden. through the analysis of over 900 full genomes from an epidemiological collection of more than 26,0 ... | 2015 | 25594904 |
| the use of coroner's autopsy reports to validate the use of targeted swabbing rather than tissue collection for rapid confirmation of virological causes of sudden death in the community. | in this study, coroner's autopsy reports were used to validate results obtained from respiratory virus screening of swabs rather than tissue collected during autopsy in cases of adult death of unknown cause. | 2015 | 25600607 |
| molecular epidemiology and phylogenetic analyses of influenza b virus in thailand during 2010 to 2014. | influenza b virus remains a major contributor to the seasonal influenza outbreak and its prevalence has increased worldwide. we investigated the epidemiology and analyzed the full genome sequences of influenza b virus strains in thailand between 2010 and 2014. samples from the upper respiratory tract were collected from patients diagnosed with influenza like-illness. all samples were screened for influenza a/b viruses by one-step multiplex real-time rt-pcr. the whole genome of 53 influenza b iso ... | 2015 | 25602617 |
| selective inactivation of usp18 isopeptidase activity in vivo enhances isg15 conjugation and viral resistance. | protein modification by the ubiquitin-like protein isg15 is an interferon (ifn) effector system, which plays a major role in antiviral defense. isg15 modification is counteracted by the isopeptidase usp18, a major negative regulator of ifn signaling, which was also shown to exert its regulatory function in an isopeptidase-independent manner. to dissect enzymatic and nonenzymatic functions of usp18 in vivo, we generated knock-in mice (usp18(c61a/c61a)) expressing enzymatically inactive usp18. usp ... | 2015 | 25605921 |
| comparison of the sofia and veritor direct antigen detection assay systems for identification of influenza viruses from patient nasopharyngeal specimens. | influenza antigen detection assays (sofia fluorescent immunoassay [fia] and veritor) yield objective results, which are potentially useful for point-of-care testing. the assays were evaluated with reverse transcriptase pcr (rt-pcr) using 411 nasopharyngeal swab specimens. sensitivity and specificity values (percentages) of 79.0/99.0 and 64.0/99.4 for influenza a and 92.9/96.7 and 78.6/98.7 for influenza b were obtained for the sofia and veritor assays, respectively. | 2015 | 25609718 |
| 2012/13 influenza vaccine effectiveness against hospitalised influenza a(h1n1)pdm09, a(h3n2) and b: estimates from a european network of hospitals. | while influenza vaccines aim to decrease the incidence of severe influenza among high-risk groups, evidence of influenza vaccine effectiveness (ive) among the influenza vaccine target population is sparse. we conducted a multicentre test-negative case-control study to estimate ive against hospitalised laboratory-confirmed influenza in the target population in 18 hospitals in france, italy, lithuania and the navarre and valencia regions in spain. all hospitalised patients aged ≥18 years, belongin ... | 2015 | 25613779 |