Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| the antileishmanial effects of lowsonia inermis and cedrus libani on leishmania major promastigotes: an in vitro study. | leishmaniasis is a parasitic disease caused by different species of protozoan parasites belonging to the genus leishmania. in this study, leishmania major (mrho/ir/75/er) promastigotes were cultured at 23-25 °c in brain heart infusion (bhi) medium supplemented with 10 % heat-inactivated fetal bovine serum (fbs) and penicillin and streptomycin. antileishmanial effects of lowsonia inermis and cedrus libani methanolic extracts (0.07, 0.15, 0.31, 0.62, 1.25, 2.5, 5, 10 mg/ml) on leishmania major pro ... | 2017 | 28615844 |
| identification of etiologic agents of cutaneous leishmaniasis in palpebral position followed by effective therapy. | cutaneous leishmaniasis (cl) is a self-healing lesion but prevention of complications and involvement of vital organs such as palpebra requires proper treatment. | 2017 | 28616411 |
| a dicre recombinase-based system for inducible expression in leishmania major. | here we present the establishment of an inducible system based on the dimerizable cre recombinase (dicre) for controlled gene expression in the protozoan parasite leishmania. rapamycin-induced dicre activation promoted efficient flipping and expression of gene products in a time and dose-dependent manner. the dicre flipping activity induced the expression of target genes from both integrated and episomal contexts broadening the applicability of the system. we validated the system by inducing the ... | 2017 | 28629935 |
| biodistribution and in vivo antileishmanial activity of 1,2-distigmasterylhemisuccinoyl-sn-glycero-3-phosphocholine liposome-intercalated amphotericin b. | 1,2-distigmasterylhemisuccinoyl-sn-glycero-3-phosphocholine (dshemspc) is a new lipid in which two molecules of stigmasterol (an inexpensive plant sterol) are covalently linked via a succinic acid to glycerophosphocholine. our previous study revealed that liposome (lip)-intercalated amphotericin b (amb) prepared from dshemspc (dshemspc-amb-lip) possesses excellent colloidal properties and in vitro antifungal and antileishmanial activities similar to those of the liposomal amb preparation ambisom ... | 2017 | 28630182 |
| a computational methodology to overcome the challenges associated with the search for specific enzyme targets to develop drugs against leishmania major. | we present an approach for detecting enzymes that are specific of leishmania major compared with homo sapiens and provide targets that may assist research in drug development. this approach is based on traditional techniques of sequence homology comparison by similarity search and markov modeling; it integrates the characterization of enzymatic functionality, secondary and tertiary protein structures, protein domain architecture, and metabolic environment. from 67 enzymes represented by 42 enzym ... | 2017 | 28638238 |
| gallic and ellagic acids: two natural immunomodulator compounds solve infection of macrophages by leishmania major. | leishmaniasis is a complex of parasitic protozoan diseases caused by more than 20 different species of parasites from leishmania genus. conventional treatments are high costly, and promote a sort of side effects. besides, protozoan resistance to treatments has been reported. natural products have been investigated as a source of new therapeutic alternatives, not only acting directly against the parasite but also being able to synergistically act on the host immune system in order to control para ... | 2017 | 28643086 |
| differential activation of human keratinocytes by leishmania species causing localized or disseminated disease. | all leishmania species parasites are introduced into mammalian skin through a sand fly bite, but different species cause distinct clinical outcomes. mouse studies suggest that early responses are critical determinants of subsequent adaptive immunity in leishmaniasis, yet few studies address the role of keratinocytes, the most abundant cell in the epidermis. we hypothesized that leishmania infection causes keratinocytes to produce immunomodulatory factors that influence the outcome of infection. ... | 2017 | 28647347 |
| divergent roles for ly6c+ccr2+cx3cr1+ inflammatory monocytes during primary or secondary infection of the skin with the intra-phagosomal pathogen leishmania major. | inflammatory monocytes can be manipulated by environmental cues to perform multiple functions. to define the role of monocytes during primary or secondary infection with an intra-phagosomal pathogen we employed leishmania major-red fluorescent protein (rfp) parasites and multi-color flow cytometry to define and enumerate infected and uninfected inflammatory cells in the skin. during primary infection, infected monocytes had altered maturation and were the initial mononuclear host cell for parasi ... | 2017 | 28666021 |
| in vitro and in vivo control of secondary bacterial infection caused by leishmania major. | bacterial infections of cutaneous leishmaniasis cause skin ulcers on mice, resulting in increased tissue deterioration, and these infections can be controlled with liquid allicin. to isolate and identify the incidences of real secondary bacterial infections in mice, we performed the current study by injecting mice (n = 50) with leishmania major. l. major infections were initiated by an intramuscular injection of 0.1 ml roswell park memorial institute (rpmi 1640 media/mouse (10(7) promastigote/ml ... | 2017 | 28703780 |
| first report on natural infection of phlebotomus sergenti with leishmania tropica in a classical focus of leishmania major in tunisia. | in tunisia, chronic cutaneous leishmaniasis due to leishmania tropica is an important health problem. its spreading has not been fully elucidated. information on sandfly vectors, as well as their associated leishmania species, is of paramount importance since vector dispersion is one of the major factors responsible for pathogen dissemination. ninety-seven unfed females belonging to the genera sergentomyia and phlebotomus were collected between june and august 2015 using sticky paper traps. poly ... | 2017 | 28719307 |
| arginase activity of leishmania isolated from patients with cutaneous leishmaniasis. | cutaneous leishmaniasis (cl) is one of the most important vector-borne parasitic diseases, highly endemic in iran, and its prevalence is increasing all over the country. arginase (arg) activity in isolated leishmania parasites from cl patients is yet to be explored. this study aimed to compare the arg activity of isolated leishmania promastigotes from cl patients with a standard strain of leishmania major and its influences on the disease pathogenesis. we recruited 16 confirmed cl patients from ... | 2017 | 28731592 |
| macromolecular biosynthetic parameters and metabolic profile in different life stages of leishmania braziliensis: amastigotes as a functionally less active stage. | it was recently hypothesized that leishmania amastigotes could constitute a semi-quiescent stage characterized by low replication and reduced metabolic activity. this concept developed with leishmania (leishmania) mexicana and leishmania (leishmania) major models might explain numerous clinical and sub-clinical features of leishmania (viannia) braziliensis infections, like reactivation of the disease, non-response to chemotherapy or asymptomatic infections. we compared here in vitro the prolifer ... | 2017 | 28742826 |
| promising antileishmanial effectiveness of doxorubicin and doxil against leishmania major: an in vitro assay. | to evaluate the effect of doxorubicin and its pegylated liposomal formulation (doxil, caelyx) on in vitro susceptibility of promastigote and amastigote stages of leishmania major. | 2017 | 28756917 |
| murine cutaneous leishmaniasis investigated by maldi mass spectrometry imaging. | imaging mass spectrometry (ims) is recognized as a powerful tool to investigate the spatial distribution of untargeted or targeted molecules of a wide variety of samples including tissue sections. leishmania is a protozoan parasite that causes different clinical manifestations in mammalian hosts. leishmaniasis is a major public health risk in different continents and represents one of the most important neglected diseases. cutaneous lesions from mice experimentally infected with leishmania spp. ... | 2017 | 28783195 |
| sensitive molecular diagnostics for cutaneous leishmaniasis. | rapid diagnosis of cutaneous leishmaniasis (cl) and identification of leishmania species is highly important for the disease management. in israel, cl is caused mainly by leishmania major and leishmania tropica species. | 2017 | 28795077 |
| cutaneous leishmaniasis in saudi arabia: a comprehensive overview. | despite the great efforts by health authorities in kingdom of saudi arabia (ksa), cutaneous leishmaniasis (cl) continues to be a major public health problem in the country. many risk factors make ksa prone to outbreaks and epidemics; among these, rapid urbanization and the huge population movement are the most important. the disease is endemic in many parts of ksa, with the majority of cases concentrated in six regions, including al-qaseem, riyadh, al-hassa, aseer, ha'il, and al-madinah. leishma ... | 2017 | 28806141 |
| structural characterization of acidic m17 leucine aminopeptidases from the tritryps and evaluation of their role in nutrient starvation in trypanosoma brucei. | leucine aminopeptidase (lap) is found in all kingdoms of life and catalyzes the metal-dependent hydrolysis of the n-terminal amino acid residue of peptide or amino acyl substrates. laps have been shown to participate in the n-terminal processing of certain proteins in mammalian cells and in homologous recombination and transcription regulation in bacteria, while in parasites, they are involved in host cell invasion and provision of essential amino acids for growth. the enzyme is essential for su ... | 2017 | 28815215 |
| chemical composition of mentha pulegium and rosmarinus officinalis essential oils and their antileishmanial, antibacterial and antioxidant activities. | the aim of the study was the determination of the chemical composition of mentha pulegium l. and rosmarinus officinalis l. essential oils and the evaluation of their antileishmanial, antibacterial and antioxidant activities. essential oils (eos) were isolated using steam distillation and the chemical composition was determined using gc-ms analysis. the antibacterial activity was tested against ten pathogenic strains using the diffusion method, the minimum inhibitory concentration (mic) and the m ... | 2017 | 28821401 |
| protein kinase c-delta (pkcδ), a marker of inflammation and tuberculosis disease progression in humans, is important for optimal macrophage killing effector functions and survival in mice. | we previously demonstrated that protein kinase c-δ (pkcδ) is critical for immunity against listeria monocytogenes, leishmania major, and candida albicans infection in mice. however, the functional relevance of pkcδ during mycobacterium tuberculosis (mtb) infection is unknown. pkcδ was significantly upregulated in whole blood of patients with active tuberculosis (tb) disease. lung proteomics further revealed that pkcδ was highly abundant in the necrotic and cavitory regions of tb granulomas in mu ... | 2017 | 28832027 |
| insights into the dynamics and dissociation mechanism of a protein redox complex using molecular dynamics. | leishmania major peroxidase (lmp) is structurally and functionally similar to the well-studied yeast cytochrome c peroxidase (ccp). a recent brownian dynamics study showed that l. major cytochrome c (lmcytc) associates with lmp by forming an initial complex with the n-terminal helix a of lmp, followed by a movement toward the electron transfer (et) site observed in the lmp-lmcytc crystal structure. critical to forming the active electron transfer complex is an intermolecular arg-asp ion pair at ... | 2017 | 28841378 |
| modeling zoonotic cutaneous leishmaniasis incidence in central tunisia from 2009-2015: forecasting models using climate variables as predictors. | transmission of zoonotic cutaneous leishmaniasis (zcl) depends on the presence, density and distribution of leishmania major rodent reservoir and the development of these rodents is known to have a significant dependence onenvironmental and climate factors. zcl in tunisia is one of the most common forms of leishmaniasis. the aim of this paper was to build a regression model of zcl cases to identify the relationship between zcl occurrence and possible risk factors, and to develop a predicting mod ... | 2017 | 28841642 |