Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients. | oral mucositis is a serious and debilitating side effect of conditioning regimens for hematopoietic stem cell transplant (hsct). through hsct, the homeostasis in the oral cavity is disrupted. the contribution of the oral microflora to mucositis remains to be clarified. the aim of our study was to investigate the relationship between yeasts, bacteria associated with periodontitis, and oral ulcerations in hsct recipients. | 2012 | 22531876 |
| serum antibodies to periodontal pathogens are a risk factor for alzheimer's disease. | chronic inflammation in periodontal disease has been suggested as a potential risk factor in alzheimer's disease (ad). the purpose of this study was to examine serum antibody levels to bacteria of periodontal disease in participants who eventually converted to ad compared with the antibody levels in control subjects. | 2012 | 22546352 |
| fusobacterium nucleatum and tannerella forsythia induce synergistic alveolar bone loss in a mouse periodontitis model. | tannerella forsythia is strongly associated with chronic periodontitis, an inflammatory disease of the tooth-supporting tissues, leading to tooth loss. fusobacterium nucleatum, an opportunistic pathogen, is thought to promote dental plaque formation by serving as a bridge bacterium between early- and late-colonizing species of the oral cavity. previous studies have shown that f. nucleatum species synergize with t. forsythia during biofilm formation and pathogenesis. in the present study, we show ... | 2012 | 22547549 |
| effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis. | emingil g, han b, özdemir g, tervahartiala t, vural c, atilla g, baylas h, sorsa t. the effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis. j periodont res 2012; 47: 729-739. © 2012 john wiley & sons a/s background and objective: to study the effectiveness of azithromycin in combination with nonsurgical periodontal therapy on clinical and microbiological parame ... | 2012 | 22571226 |
| bacterial pathogens related to chronic suppurative otitis media in individuals with cleft palate: bacteriological culture and polymerase chain reaction. | to characterize the microbial etiology of chronic suppurative otitis media comparing the methods of classical bacteriological culture and polymerase chain reaction. | 2014 | 22594401 |
| septic arthritis and osteomyelitis in a 10-year-old boy, caused by fusobacterium nucleatum, diagnosed with pcr/16s ribosomal bacterial dna amplification. | a 10-year-old boy presented with an atypical non-febrile septic arthritis/osteomyelitis. he was unresponsive to routine antibiotic treatment with flucloxacillin/gentamicin as the pain and fluid collection increased. synovial fluid cultures are negative and gram stain remained negative. only after pcr/16s ribosomal bacterial dna amplification a fusobacterium nucleatum could be detected, and antibiotic therapy switched to clindamycin with rapid response. septic osteomyelitis and arthritis are rela ... | 2012 | 22605875 |
| relationship between oral anaerobic bacteria and otitis media with effusion. | in this study hypothesing the translocation of oral bacteria from oropharynx into the middle ear cavity may be involved in the pathogenesis of otitis media with effusion (ome), we aimed to investigate the presence and similarity of fusobacterium nucleatum and treponema denticola in saliva, nasopharyngeal secretion and the middle ear effusion samples from the children with ome. | 2012 | 22606045 |
| the role of coaggregation between porphyromonas gingivalis and fusobacterium nucleatum on the host response to mixed infection. | to evaluate the role of coaggregation between porphyromonas gingivalis and fusobacterium nucleatum on the virulence of the mixed infection in mice. | 2012 | 22607053 |
| neutrophil superoxide production in the presence of cigarette smoke extract, nicotine and cotinine. | to determine the effect of cigarette smoke extract, nicotine and cotinine on lucigenin-detectable neutrophil superoxide production. | 2012 | 22607095 |
| occurrence and antimicrobial susceptibility of porphyromonas spp. and fusobacterium spp. in dogs with and without periodontitis. | the occurrence of porphyromonas gulae, porphyromonas macacae, fusobacterium nucleatum and fusobacterium canifelinum in subgingival plaque from dogs with and without periodontitis as well as their antimicrobial susceptibility were evaluated. from 50 dogs with periodontitis were identified 38 p. gulae, 8 p. macacae, 26 f. nucleatum and 15 f. canifelinum, and from 50 dogs without periodontitis were identified 15 p. gulae, 12 f. nucleatum and 11 f. canifelinum. all strains were susceptible to most o ... | 2012 | 22609780 |
| microbial diversity in failed endodontic root-filled teeth. | persistent/secondary infections of human root canals play an important role in the failure of endodontic treatment. this study used 16s rrna sequencing to assess microbial diversity in root-filled teeth associated with failed endodontic treatment. | 2012 | 22613548 |
| [impact of periodontal disease on arterial pressure in diabetic mice]. | diabetes-driven cardiovascular diseases represent a high challenge for developed countries. periodontal disease is strictly linked to the aforementioned diseases, due to its gram negative-driven inflammation. thus, we investigated the effects of periodontal disease on arterial pressure during the development of diabetes in mice. to this aim, c57bl/6 female mice were colonized with pathogens of periodontal tissue (porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum) for 1m ... | 2012 | 22621847 |
| the foma porin from fusobacterium nucleatum is a toll-like receptor 2 agonist with immune adjuvant activity. | many bacterial components selectively activate immune and nonhematopoietic target cells via toll-like receptor (tlr) signaling; modulation of such host responses defines the immune adjuvant properties of these bacterial products. for example, the outer membrane protein porins from neisseria, salmonella, and shigella are known tlr2 agonists with established systemic and mucosal immune adjuvanticity. early work indicated that the foma porin from fusobacterium nucleatum has immune adjuvant activity ... | 2012 | 22623652 |
| pulmonary botryomycosis on a lung cavity: a rare pulmonary infection mimicking cancer. | lung botryomycosis is a rare disease. we report what is to our knowledge the first case occurring on a lung cavity. in a 42-year-old man suffering asthenia and cough, a chest radiograph revealed a right upper lobe opacity. computed tomography scan showed a necrotic mass which was also spiculated. repeated research for mycobacterium tuberculosis was negative. the patient underwent a lobectomy. histological and bacteriological examinations made the diagnosis of botryomycosis, because the cavity pr ... | 2012 | 22627958 |
| autoinducer 2 of fusobacterium nucleatum as a target molecule to inhibit biofilm formation of periodontopathogens. | periodontitis is initiated by bacteria in subgingival biofilms, which are composed mostly of gram-negative anaerobes. autoinducer 2 (ai-2) is a universal quorum sensing (qs) molecule that mediates intergeneric signalling in multispecies bacterial communities and may induce biofilm formation. as fusobacterium nucleatum is the major coaggregation bridge organism that links early colonising commensals and late pathogenic colonisers in dental biofilms via the accretion of periodontopathogens, we hyp ... | 2013 | 22633049 |
| co-localized or randomly distributed? pair cross correlation of in vivo grown subgingival biofilm bacteria quantified by digital image analysis. | the polymicrobial nature of periodontal diseases is reflected by the diversity of phylotypes detected in subgingival plaque and the finding that consortia of suspected pathogens rather than single species are associated with disease development. a number of these microorganisms have been demonstrated in vitro to interact and enhance biofilm integration, survival or even pathogenic features. to examine the in vivo relevance of these proposed interactions, we extended the spatial arrangement analy ... | 2012 | 22655057 |
| [lemierre's syndrome as differential diagnosis of lung cancer]. | lemierre's syndrome is a disseminated infection which is usually caused by fusobacterium necrophorum. an oropharyngeal infection progresses to a septic thrombophlebitis of the internal jugular vein and later metastatic infections throughout the body occur. we present a clinical case in which a patient, initially presenting with symptoms characteristic of pulmonary cancer, turned out to have a rare variant of lemierre's syndrome caused by fusobacterium nucleatum. | 2012 | 22668649 |
| comprehensive analysis of bacterial flora in postoperative maxillary cyst fluid by 16s rrna gene and culture methods. | intracystic fluid was aseptically collected from 11 patients with postoperative maxillary cyst (pomc), and dna was extracted from the pomc fluid. bacterial species were identified by sequencing after cloning of approximately 580 bp of the 16s rrna gene. identification of pathogenic bacteria was also performed by culture methods. the phylogenetic identity was determined by sequencing 517-596 bp in each of the 1139 16s rrna gene clones. a total of 1114 clones were classified while the remaining 25 ... | 2012 | 22685668 |
| effect of s-prg eluate on biofilm formation and enzyme activity of oral bacteria. | recently, the antibacterial activity of a composite resin containing prereacted glass ionomer (s-prg) filler was revealed. we examined the effect of an s-prg eluate on various biologic activities of streptococcus mutans and porphyromonas gingivalis. adherence ability of s. mutans was evaluated by microtiter plate assay; protease and gelatinase activities of p. gingivalis were examined by synthetic substrate hydrolysis and gelatin film spot assay, respectively. coaggregation of p. gingivalis with ... | 2012 | 22693508 |
| a microbiological profile of unexposed and exposed pulp space of primary endodontic infections by checkerboard dna-dna hybridization. | the aim of this study was to evaluate, by checkerboard dna-dna hybridization, the composition of the microbiota of primary endodontic infections in cases associated with exposed (n = 30) and unexposed (n = 30) pulp space. | 2012 | 22703649 |
| peritonsillar abscess: treatment with immediate tonsillectomy - 10 years of experience. | immediate tonsillectomy in patients with peritonsillar abscess is a safe and effective treatment that should be considered as an alternative to conventional incision and drainage. | 2012 | 22708633 |
| subgingival microbiota from cebus apella (capuchin monkey) with different periodontal conditions. | this present study evaluated the subgingival microbiota of the cebus apella with different periodontal conditions kept by the tufted capuchin monkey procreation center (são paulo state university - unesp) or free-ranging monkeys. for this purpose, clinical specimens of subgingival biofilm were collected from 52 monkeys, of both genders, 40 kept in captivity and 12 free-ranging monkeys. the primates were submitted to periodontal evaluation and biofilm samples were transferred to vmga iii transpor ... | 2012 | 22710412 |
| antimicrobial activity of nanoparticulate metal oxides against peri-implantitis pathogens. | dental plaque accumulation may result in peri-implantitis, an inflammatory process causing loss of supporting bone that may lead to dental implant failure. the antimicrobial activities of six metal and metal oxide nanoparticles and two of their composites against bacterial pathogens associated with peri-implantitis were examined under anaerobic conditions. the activities of nanoparticles of silver (ag), cuprous oxide (cu(2)o), cupric oxide (cuo), zinc oxide (zno), titanium dioxide (tio(2)), tung ... | 2012 | 22727529 |
| subgingival biodiversity in subjects with uncontrolled type-2 diabetes and chronic periodontitis. | there is a bidirectional relationship between periodontal disease and type-2 diabetes mellitus (dm). inflammatory mediators may negatively affect glycemic control, and increased glucose levels and resultant glycation end-products may alter the host response against bacterial infection. however, no agreement has been reached regarding the effect of dm on periodontal subgingival microbiota. therefore, the purpose of the present study was to compare the subgingival biodiversity in deep periodontal ... | 2013 | 22762355 |
| strain-specific colonization patterns and serum modulation of multi-species oral biofilm development. | periodontitis results from an ecological shift in the composition of subgingival biofilms. subgingival community maturation is modulated by inter-organismal interactions and the relationship of communities with the host. in an effort to better understand this process, we evaluated biofilm formation, with oral commensal species, by three strains of the subgingivally prevalent microorganism fusobacterium nucleatum and four strains of the periodontopathogen porphyromonas gingivalis. we also tested ... | 2012 | 22771792 |
| effectiveness of egg yolk immunoglobulin (igy) against periodontal disease-causing fusobacterium nucleatum. | to evaluate the in vitro and in vivo effectiveness of egg yolk immunoglobulin (igy) against periodontal disease-causing fusobacterium nucleatum. | 2012 | 22789022 |
| comparison of endotoxin levels found in primary and secondary endodontic infections. | this clinical study was conducted to compare the levels of endotoxins (lipopolysaccharides [lpss]) found in primary and secondary endodontic infections with apical periodontitis by correlating lps contents with clinical/radiographic findings. in addition, the presence of target gram-negative anaerobic bacteria was also investigated. | 2012 | 22794210 |
| efficacy of berberine, an antimicrobial plant alkaloid, as an endodontic irrigant against a mixed-culture biofilm in an in vitro tooth model. | berberine, a plant alkaloid isolated from many medicinal plants, has shown antimicrobial activity against selected oral pathogens. the purpose of this investigation was to evaluate the antimicrobial efficacy of berberine solution against selected endodontic pathogens using a multispecies biofilm tooth model. | 2012 | 22794217 |
| antibacterial effects of blackberry extract target periodontopathogens. | antimicrobial agents provide valuable adjunctive therapy for the prevention and the control of oral diseases. limitations in their prolonged use have stimulated the search for new, naturally occurring agents with more specific activity and fewer adverse effects. here we sought to determine the antibacterial properties of blackberry extract (bbe) in vitro against oral bacterial commensals and periodontopathogens. | 2012 | 22812456 |
| osteomyelitis of a long bone due to fusobacterium nucleatum and actinomyces meyeri in an immunocompetent adult: a case report and literature review. | fusobacterium species are uncommon causes of osteomyelitis. these organisms are normal flora of the oral cavity. therefore, they mostly cause osteomyelitis of the head and neck. hematogenous osteomyelitis at distant sites other than the head and neck has rarely been reported in pediatric or immunocompromised patients. here, we report the first case of osteomyelitis of a long bone combined with a muscle abscess due to fusobacterium nucleatum in an otherwise healthy adult. | 2012 | 22817336 |
| porphyromonas gingivalis mediates inflammasome repression in polymicrobial cultures through a novel mechanism involving reduced endocytosis. | the interleukin (il)-1β-processing inflammasome has recently been identified as a target for pathogenic evasion of the inflammatory response by a number of bacteria and viruses. we postulated that the periodontal pathogen, porphyromonas gingivalis may suppress the inflammasome as a mechanism for its low immunogenicity and pathogenic synergy with other, more highly immunogenic periodontal bacteria. our results show that p. gingivalis lacks signaling capability for the activation of the inflammaso ... | 2012 | 22843689 |
| fusobacterium nucleatum infection mimicking metastatic cancer. | a 48-year-old man presented with fevers, chills, weight loss, multiple liver masses, and several superficial and deep venous thromboses in lower extremities. cancer work up was negative. a liver biopsy grew fusobacterium nucleatum. to our knowledge, f. nucleatum infection presenting with multiple liver masses and trousseau-like syndrome has not been reported earlier. | 2012 | 22875742 |
| periodontal pathogen load and increased antibody response to heat shock protein 60 in patients with cardiovascular disease. | to determine the relationship between periodontal pathogen load and anti-human heat shock protein 60 (hhsp60) antibodies in patients with established cardiovascular disease (cvd). | 2012 | 22882677 |
| antibiotic susceptibility profiles of oral pathogens. | periodontitis is a bacterial disease that can be treated with systemic antibiotics. the aim of this study was to establish the antibiotic susceptibility profiles of five periodontal pathogens to six commonly used antibiotics in periodontics. a total of 247 periodontal bacterial isolates were tested for susceptibility to the six antibiotics using the etest method. mic(50) and mic(90) values (minimum inhibitory concentrations for 50% and 90% of the organisms, respectively) were calculated. both eu ... | 2012 | 22890195 |
| micronutrient modulation of nf-κb in oral keratinocytes exposed to periodontal bacteria. | chronic periodontal diseases are characterised by a dysregulated and exaggerated inflammatory/immune response to plaque bacteria. we have demonstrated previously that oral keratinocytes up-regulate key molecular markers of inflammation, including nf-κb and cytokine signalling, when exposed to the periodontal bacteria porphyromonas gingivalis and fusobacterium nucleatum in vitro. the purpose of the current study was to investigate whether α-lipoic acid was able to abrogate bacterially-induced pro ... | 2013 | 22890546 |
| bacterial antagonism against periodontopathogens. | the aim of the current study is to compare the prevalence of commensal bacteria, with beneficial properties, for healthy and diseased individuals and additionally to examine the inhibitory effect of some commercial dietary probiotics on periodontopathogens, comparing this inhibitory effect to that of orally derived beneficial bacteria. | 2013 | 22897652 |
| ascorbate and α-tocopherol differentially modulate reactive oxygen species generation by neutrophils in response to fcγr and tlr agonists. | periodontitis, a ubiquitous chronic inflammatory disease, is associated with reduced antioxidant defences and neutrophil hyperactivity in terms of reactive oxygen species (ros) generation. its phenotype is thus characterized by oxidative stress. we have determined the effect of antioxidant micronutrients ascorbate and α-tocopherol on neutrophil ros generation. peripheral neutrophils from periodontally-healthy individuals (n = 20) were challenged with phorbol myristate acetate, igg-opsonised stap ... | 2013 | 22914919 |
| a proteomic investigation of fusobacterium nucleatum alkaline-induced biofilms. | the gram negative anaerobe fusobacterium nucleatum has been implicated in the aetiology of periodontal diseases. although frequently isolated from healthy dental plaque, its numbers and proportion increase in plaque associated with disease. one of the significant physico-chemical changes in the diseased gingival sulcus is increased environmental ph. when grown under controlled conditions in our laboratory, f. nucleatum subspecies polymorphum formed mono-culture biofilms when cultured at ph 8.2. ... | 2012 | 22943491 |
| draft genome sequence of fusobacterium nucleatum subsp. fusiforme atcc 51190t. | fusobacterium nucleatum, one of the major causative bacteria of periodontitis, is classified into five subspecies (nucleatum, polymorphum, vincentii, animalis, and fusiforme) on the basis of the several phenotypic characteristics and dna homology. this is the first report of the draft genome sequence of f. nucleatum subsp. fusiforme atcc 51190(t). | 2012 | 22965077 |
| isothermal microcalorimetry provides new insights into biofilm variability and dynamics. | the purpose of this study was to investigate a three-species in vitro biofilm with peri-implantitis-related bacteria for its variability and metabolic activity. streptococcus sanguinis, fusobacterium nucleatum, and porphyromonas gingivalis were suspended in simulated body fluid containing 0.2% glucose to form biofilms on polished, protein-coated implant-grade titanium disks over 72 h using a flow chamber system. thereafter, biofilm-coated disks were characterized by scanning electron microscopy ... | 2012 | 22967269 |
| proteomics of streptococcus gordonii within a model developing oral microbial community. | streptococcus gordonii is one of several species that can initiate the formation of oral biofilms that develop into the complex multispecies microbial communities referred to as dental plaque. it is in the context of dental plaque that periodontal pathogens such as porphyromonas gingivalis cause disease. we have previously reported a whole cell quantitative proteomics investigation of p. gingivalis in a model dental plaque community of s. gordonii, p. gingivalis, and fusobacterium nucleatum. her ... | 2012 | 22989070 |
| maternal oral origin of fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16s-23s rrna gene intergenic transcribed spacer region. | fusobacterium nucleatum, a common gram-negative anaerobe prevalent in the oral cavity, possesses the ability to colonize the amniotic cavity and the fetus. however, f. nucleatum may also be part of the vaginal microbiota from where it could reach the amniotic tissues. due to the heterogeneity of f. nucleatum, consisting of five subspecies, analysis at the subspecies/strain level is desirable to determine its precise origin. the aims of this study were: (i) to evaluate the use of the 16s-23s rrna ... | 2013 | 23002071 |
| detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women. | the aim of this study was to use the fluorescence in situ hybridization (fish) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. this cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque samples were collected and the fish ... | 2012 | 23018230 |
| morphological, biochemical, physiological and molecular aspects of the response of fusobacterium nucleatum exposed to subinhibitory concentrations of antimicrobials. | subinhibitory concentrations (sics) of antimicrobials may result in alterations in bacterial biology with implications for its potential aggression. this has considerable importance for the resident microbiota. our aim was to analyze the effects of sics of antimicrobials on the morphological, biochemical, physiological and molecular characteristics of the resident anaerobic fusobacterium nucleatum. fourteen strains were obtained from f. nucleatum atcc 25586, selected by culturing on sics of ampi ... | 2012 | 23023263 |
| protective potential of non-dialyzable material fraction of cranberry juice on the virulence of p. gingivalis and f. nucleatum mixed infection. | periodontitis is a polymicrobial infectious disease. a novel potential chemical treatment modality may lie in bacterial anti-adhesive materials, such as cranberry juice fractions. the aim of this study is to explore the effect of high molecular weight cranberry constituent (non-dialyzable material [ndm]) on the virulence of a mixed infection with porphyromonas gingivalis and fusobacterium nucleatum in mice. | 2013 | 23030239 |
| porphyromonas gingivalis entry into gingival epithelial cells modulated by fusobacterium nucleatum is dependent on lipid rafts. | host cell invasion by a major periodontal pathogen, porphyromonas gingivalis, has been proposed as an important mechanism involved in host-pathogen interactions in periodontal and cardiovascular diseases. the present study sought to gain insight into the underlying mechanism(s) involved in previously demonstrated fusobacterial modulation of host cell invasion by p. gingivalis. an immortalized human gingival cell line ca9-22 was dually infected with p. gingivalis atcc 33277 and fusobacterium nucl ... | 2012 | 23034475 |
| microscope-based imaging platform for large-scale analysis of oral biofilms. | a microscopic method for noninvasively monitoring oral biofilms at the macroscale was developed to describe the spatial distribution of biofilms of different bacterial composition on bovine enamel surfaces (bes). for this purpose, oral biofilm was grown in situ on bes that were fixed at approximal sites of individual upper jaw acrylic devices worn by a volunteer for 3 or 5 days. eubacteria, streptococcus spp., and fusobacterium nucleatum were stained using specific fluorescence in situ hybridiza ... | 2012 | 23042171 |
| antibacterial activity of myristica fragrans against oral pathogens. | myristica fragrans houtt is mostly cultivated for spices in penang island, malaysia. the ethyl acetate and ethanol extracts of flesh, mace and seed of myristica fragrans was evaluated the bactericidal potential against three gram-positive cariogenic bacteria (streptococcus mutans atcc 25175, streptococcus mitis atcc 6249, and streptococcus salivarius atcc 13419) and three gram-negative periodontopathic bacteria (aggregatibacter actinomycetemcomitans atcc 29522, porphyromonas gingivalis atcc 3327 ... | 2012 | 23049613 |
| antibacterial activity of calcium hydroxide and chlorhexidine containing points against fusobacterium nucleatum and parvimonas micra. | up to 90% of the flora of an infected root canal consists of obligate anaerobic bacteria. however, most studies have centred on microaerophiles. this quantitative assay investigated the antibacterial effects against fusobacterium nucleatum and parvimonas micra of gutta-percha (gp) points containing either calcium hydroxide (ca(oh)(2)) or chlorhexidine (chx) compared to those of conventional gp points. | 2012 | 23077425 |
| inflammatory index and treatment of brain abscess. | this study retrospectively analyzed 12 patients with brain abscesses. half of the patients were diagnosed inaccurately in the initial stage, and 7.2 days were required to achieve the final diagnosis of brain abscess. the patients presented only with a moderately elevated leukocyte count, serum crp levels, or body temperatures during the initial stage. these markers changed, first with an increase in the leukocyte count, followed by the crp and body temperature. the degree of elevation tended to ... | 2012 | 23092104 |
| draft genome sequence of fusobacterium nucleatum chdc f128, isolated from a periodontitis lesion. | fusobacterium nucleatum is classified into five subspecies. f. nucleatum chdc f128 was isolated from a periodontitis lesion and proposed as a new subspecies based on the comparison of the nucleotide sequences of the rna polymerase beta subunit and zinc protease genes. here, we report the draft genome sequence of the strain. | 2012 | 23105064 |
| effects of cranberry components on human aggressive periodontitis gingival fibroblasts. | aggressive periodontitis (agp) causes rapid periodontal breakdown involving agp gingival fibroblast production of cytokines [i.e. interleukin (il)-6, a bone metabolism regulator], and matrix metalloproteinase (mmp)-3. lipopolysaccharide upregulates fibroblast il-6 and mmp-3, via transcription factors (i.e. nf-κb). cranberry (vaccinium macrocarpon) inhibits lipopolysaccharide-stimulated macrophage and normal gingival fibroblast activities, but little is known of its effects on agp fibroblasts. ob ... | 2013 | 23106206 |
| acute chorioamnionitis at term caused by the oral pathogen fusobacterium nucleatum. | fusobacterium nucleatum is an oral pathogen associated with preterm birth. presented is a case of acute chorioamnionitis that progressed to maternal sepsis in a term patient with intact membranes. in addition to its role in periodontal disease and preterm birth, our case demonstrates that intrauterine infection with fusobacterium nucleatum can result in severe disease at term. | 2012 | 23115747 |
| toll-like receptor 2-dependent activity of native major outer membrane protein proteosomes of chlamydia trachomatis. | chlamydia trachomatis is the most common sexually transmitted bacterial pathogen and the etiologic agent of blinding trachoma. intracellular signaling pathways leading to host cell inflammation and innate immunity to chlamydia include those mediated by toll-like receptors (tlrs) and nucleotide binding oligomerization domain 1 (nod1) protein. in epithelial cells, tlr-dependent signaling contributes to local immune responses via induction of inflammatory mediators. there is evidence that tlr3, tlr ... | 2013 | 23132491 |
| diagnosis and treatment of fusobacterium nucleatum discitis and vertebral osteomyelitis: case report and review of the literature. | case report. | 2013 | 23132536 |
| high-fat diet induces periodontitis in mice through lipopolysaccharides (lps) receptor signaling: protective action of estrogens. | a fat-enriched diet favors the development of gram negative bacteria in the intestine which is linked to the occurrence of type 2 diabetes (t2d). interestingly, some pathogenic gram negative bacteria are commonly associated with the development of periodontitis which, like t2d, is characterized by a chronic low-grade inflammation. moreover, estrogens have been shown to regulate glucose homeostasis via an lps receptor dependent immune-modulation. in this study, we evaluated whether diet-induced m ... | 2012 | 23133617 |
| prevalence of β-lactamase-producing bacteria in human periodontitis. | beta-lactam antibiotics prescribed in periodontal therapy are vulnerable to degradation by bacterial β-lactamases. this study evaluated the occurrence of β-lactamase-positive subgingival bacteria in chronic periodontitis subjects of usa origin, and assessed their in vitro resistance to metronidazole at a breakpoint concentration of 4 μg/ml. | 2013 | 23173872 |
| purification, crystallization and preliminary x-ray analysis of two hydrogen sulfide-producing enzymes from fusobacterium nucleatum. | hydrogen sulfide produced by oral bacteria is responsible for oral malodour. two homologous hydrogen sulfide-producing enzymes, fn1220 and cdl, from fusobacterium nucleatum (which actively produces hydrogen sulfide) were overproduced, purified and crystallized. x-ray diffraction data were collected from the crystals using a synchrotron-radiation source. the fn1220 crystal belonged to tetragonal space group p4(1)2(1)2 or p4(3)2(1)2 (unit-cell parameters a=b=116.8, c=99.2 å) and the cdl crystal be ... | 2012 | 23192034 |
| optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogens. | the aim of this study was to optimize quantitative (real-time) polymerase chain reaction (qpcr) assays for 8 major periodontal pathogens, i.e. aggregatibacter actinomycetemcomitans, fusobacterium nucleatum, parvimonas micros, porphyromonas gingivalis, prevotella intermedia, tanerella forsythia and treponema denticola, and of the caries pathogen streptococcus mutans. | 2012 | 23199017 |
| antibacterial activity of probiotic candidates for oral health. | the aim of this study was to determine the probiotic potential of autochthonous oral lactobacilli. for this, 66 strains were screened for antibacterial activity against two cariogenic strains (streptococcus mutans and actinomyces viscosus) and two periodontopathogenic strains (fusobacterium nucleatum and porphyromonas gingivalis). the inhibitory activity was investigated with the agar overlay technique. positive results led us to explore some mechanisms of action. the ability to produce h(2)o(2) ... | 2013 | 23211763 |
| modulation of quaternary structure and enhancement of ligand binding by the k-turn of tandem glycine riboswitches. | most known glycine riboswitches have two homologous aptamer domains arranged in tandem and separated by a short linker. the two aptamers associate through reciprocal "quaternary" interactions that have been proposed to result in cooperative glycine binding. recently, the interaptamer linker was found to form helix p0 with a previously unrecognized segment 5' to the first aptamer domain. p0 was shown to increase glycine affinity, abolish cooperativity, and conform to the k-turn motif consensus. w ... | 2013 | 23249744 |
| virulence mechanism of bacteria in mixed infection: attenuation of cytokine levels and evasion of polymorphonuclear leukocyte phagocytosis. | the objective of the present study is to evaluate the effect of bacterial viability on the virulence of mixed infection. | 2013 | 23259412 |
| fusobacterium nucleatum subsp. fusiforme gharbia and shah 1992 is a later synonym of fusobacterium nucleatum subsp. vincentii dzink et al. 1990. | on the basis of the dna-dna hybridization patterns and phenotypic characteristics, fusobacterium nucleatum was classified into five subspecies. previous studies have suggested that f. nucleatum subsp. vincentii is genetically similar to f. nucleatum subsp. fusiforme. the aim of this study was to investigate the possibility of classifying these two subspecies into a single subspecies by phylogenetic analysis using a single sequence (24,715 bp) concatenated 22 housekeeping genes of eight f. nuclea ... | 2013 | 23263257 |
| validation of a quantitative real-time pcr assay and comparison with fluorescence microscopy and selective agar plate counting for species-specific quantification of an in vitro subgingival biofilm model. | subgingival biofilms are the prime etiological factor of periodontal disease. owing to their complex polymicrobial nature, quantification of individual bacterial species within the biofilm for research and diagnostic purposes can be methodologically challenging. the aims of this study were to establish a quantitative real-time pcr (qpcr) assay to quantify the bacteria used in our 10-species in vitro 'subgingival' biofilm model and to compare the quantitative outcome with fluorescence microscopy ... | 2012 | 23278531 |
| variations in the oral anaerobic microbial flora in relation to pregnancy. | pregnancy gingivitis is a major oral infection. periodontium acts as a reservoir of inflammatory mediators and sub gingival biofilms of bacteria. | 2012 | 23285437 |
| method to quantify live and dead cells in multi-species oral biofilm by real-time pcr with propidium monoazide. | real-time pcr (qpcr) is a widely used technique in analysing environmental and clinical microbiological samples. however, its main limitation was its inability to discriminate between live and dead cells.recently, propidium monoazide (pma) together with qpcr has been used to overcome this problem, with good results for different bacterial species in different types of samples.our objective was to implement this technique for analysing mortality in multi-species oral biofilms formed in vitro with ... | 2013 | 23289803 |
| [expression of cytokines in dentogingival sulcus in patients after dental implantation and during development of peri-implantitis]. | determination of cytokine content in various areas of dentition in patients with peri-implantitis associated with parodontopathogenic bacteria species of i and ii order. | 2012 | 23297645 |
| oral epithelial cell responses to multispecies microbial biofilms. | this report describes the use of a novel model of multispecies biofilms to stimulate profiles of cytokines/chemokines from oral epithelial cells that contribute to local inflammation in the periodontium. streptococcus gordonii (sg)/s. oralis (so)/s. sanguinis (ss) and sg/fusobacterium nucleatum (fn)/porphyromonas gingivalis (pg) biofilms elicited significantly elevated levels of il-1α and showed synergistic stimulatory activity compared with an additive effect of the 3 individual bacteria. only ... | 2013 | 23300185 |
| bacterial adhesion on the titanium and zirconia abutment surfaces. | microorganisms harboring the oral cavity, mainly those related to periodontal diseases, are the most potential etiologic factor of failure in long-term implant treatment. the material used for abutment components may influence the adhesion and colonization of microbial species. the aim of this in vivo investigation was to evaluate the biofilm formation on machined (mpt) or cast titanium (cpt) and zirconia abutments (zc). | 2014 | 23316996 |
| dna binding to hydroxyapatite: a potential mechanism for preservation of microbial dna. | molecular methods are increasingly being deployed for analysis of the microbial flora in the root canal. such methods are based on the assumption that recovered dna is associated with the active endodontic infection, yet paleomicrobiology research is based on the recovery of ancient dna from centuries-old tooth and bone samples, which points to considerable longevity of the dna molecule in these tissues. the main component of dentin and bone is the mineral hydroxyapatite. this study assessed dna ... | 2013 | 23321233 |
| exploiting preq(1) riboswitches to regulate ribosomal frameshifting. | knowing the molecular details of the interaction between riboswitch aptamers and their corresponding metabolites is important to understand gene expression. here we report on a novel in vitro assay to study preq(1) riboswitch aptamers upon binding of 7-aminomethyl-7-deazaguanine (preq(1)). the assay is based on the ability of the preq(1) aptamer to fold, upon ligand binding, into a pseudoknotted structure that is capable of stimulating -1 ribosomal frameshifting (-1 fs). aptamers from three diff ... | 2013 | 23327288 |
| clinical and microbiological results following nonsurgical periodontal therapy with or without local administration of piperacillin/tazobactam. | we assessed if adjunct administration of piperacillin/tazobactam added clinical and microbiological treatment benefits. | 2013 | 23334268 |
| antibody and t cell responses to fusobacterium nucleatum and treponema denticola in health and chronic periodontitis. | the characteristics of the t cell response to the members of oral flora are poorly understood. we characterized the antibody and t cell responses to fada and td92, adhesins from fusobacterium nucleatum, an oral commensal, and treponema denticola, a periodontal pathogen, respectively. peripheral blood and saliva were obtained from healthy individuals and patients with untreated chronic periodontitis (cp, n = 11 paris) and after successful treatment of the disease (n = 9). the levels of antigen-sp ... | 2013 | 23335969 |
| characteristics of porphyromonas gingivalis lipopolysaccharide in co-culture with fusobacterium nucleatum. | porphyromonas gingivalis is associated with chronic periodontitis and forms multi-species biofilms. they can communicate within species as well as with other species found in the subgingiva, which may induce changes in the growth ratio and virulence of periodontopathogens. the lipopolysaccharide (lps) of p. gingivalis shows different virulence by growth condition. the purpose of this study was to investigate the characteristics of p. gingivalis lps when co-cultured with fusobacterium nucleatum. ... | 2013 | 23347381 |
| the surface layer of tannerella forsythia contributes to serum resistance and oral bacterial coaggregation. | tannerella forsythia is an anaerobic, gram-negative bacterium involved in the so-called "red complex," which is associated with severe and chronic periodontitis. the surface layer (s-layer) of t. forsythia is composed of cell surface glycoproteins, such as tfsa and tfsb, and is known to play a role in adhesion/invasion and suppression of proinflammatory cytokine expression. here we investigated the association of this s-layer with serum resistance and coaggregation with other oral bacteria. the ... | 2013 | 23357386 |
| in vitro antimicrobial effects of two antihalitosis mouth rinses on oral pathogens and human tongue microbiota. | the aim of the study was to compare the antimicrobial activity of a mouth rinse containing chlorhexidine and cetylpyridinium chloride (mr1) with a stannous fluoride-based mouth rinse (mr2) in vitro. | 2013 | 23368885 |
| folding and stability of outer membrane protein a (ompa) from escherichia coli in an amphipathic polymer, amphipol a8-35. | amphipols are a class of amphipathic polymers designed to maintain membrane proteins in aqueous solutions in the absence of detergents. denatured β-barrel membrane proteins, like outer membrane proteins ompa from escherichia coli and foma from fusobacterium nucleatum, can be folded by dilution of the denaturant urea in the presence of amphipol a8-35. here, the folding kinetics and stability of ompa in a8-35 have been investigated. folding is well described by two parallel first-order processes, ... | 2013 | 23370791 |
| characterization of extracellular polymeric matrix, and treatment of fusobacterium nucleatum and porphyromonas gingivalis biofilms with dnase i and proteinase k. | biofilms are organized communities of microorganisms embedded in a self-produced extracellular polymeric matrix (epm), often with great phylogenetic variety. bacteria in the subgingival biofilm are key factors that cause periodontal diseases; among these are the gram-negative bacteria fusobacterium nucleatum and porphyromonas gingivalis. the objectives of this study were to characterize the major components of the epm and to test the effect of deoxyribonuclease i (dnase i) and proteinase k. | 2013 | 23372876 |
| macrolides inhibit fusobacterium nucleatum-induced muc5ac production in human airway epithelial cells. | fusobacterium nucleatum is one of the most common anaerobic bacteria in periodontitis and is responsible for several extraoral infections, including respiratory tract diseases. in this study, we examined whether f. nucleatum induces mucin secretion in airway epithelial cells. we also examined the effects of macrolides on f. nucleatum-induced mucus production compared with the effects of other antibiotics that exert anti-anaerobic activities. the production of muc5ac, the major core protein of mu ... | 2013 | 23380724 |
| regulation of visfatin by microbial and biomechanical signals in pdl cells. | this in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (pdl) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals. | 2014 | 23404558 |
| microbiology of the gut of the kola nut weevil, balanogastris kolae. | reports have shown that many insects have microbes in their gut system. gut microbes are very important for insect vitality and much of their nutrition is derived from products of microbial metabolism. the habitat of balanogastris kolae (desbrocher des loges) (coleoptera: curculionidae) suggests that they possess the ability to digest varieties of sugars particularly starch and protein materials present in the kola nut, cola nitida schott & endlicher (malvales: malvaceae). the aim of this study ... | 2012 | 23421598 |
| identification of a 43-kda outer membrane protein of fusobacterium necrophorum that exhibits similarity with pore-forming proteins of other fusobacterium species. | a pair of primers was designed in an attempt to amplify outer membrane protein (omp) gene of fusobacterium necrophorum based on nucleotide sequence of the omp of fusobacterium nucleatum. further analysis was performed to characterize its molecular properties and phylogeny in the genus fusobacterium. we identified a predicated 43kda outer membrane protein (43k omp) in f. necrophorum, which showed the same properties as other pore-forming proteins of gram-negative anaerobic bacteria according to a ... | 2013 | 23433684 |
| comparative microbial analysis of paired amniotic fluid and cord blood from pregnancies complicated by preterm birth and early-onset neonatal sepsis. | 16s rrna-based genomic analyses have revolutionized our understanding of infectious diseases. many cases which were recognized as "idiopathic" are now known to have an infectious etiology. here, we present a proof-of-concept study to examine the microbial link between intra-amniotic infection (iai) and early-onset neonatal sepsis (eons). | 2013 | 23437088 |
| peptides: β-cyclodextrin inclusion compounds as highly effective antimicrobial and anti-epithelial proliferation agents. | the use of antimicrobial peptides (amps) as therapeutic agents for periodontal infections has great advantages, such as broad spectrum of action, low toxicity, and limited bacterial resistance. however, their practical use is limited because of the large amount of peptide required to exercise the microbicidal function. | 2013 | 23510146 |
| antibacterial activity of essential oils against periodontal pathogens: a qualitative systematic review. | periodontal diseases are among the most common infectious diseases that lead to the destruction of periodontal tissues. anaerobic gram-negative bacteria (aggregatibacter actinomecetemcomitans, porphyromonas gingivalis, fusobacterium nucleatum...) isolated from periodontal lesions, have been shown to be related to the onset and progression of periodontal disease. given the incidence of periodontitis, increased resistance of oral bacteria to antibiotics and adverse effects of some antibacterial ag ... | 2012 | 23513511 |
| antibacterial effect of different root canal sealers on three bacterial species. | bacteria persisting in the root canal system may thwart endodontic therapy. it is therefore interesting to know whether clinically available root canal sealers have an antimicrobial effect. the objective of the present in vitro study was to investigate the antibacterial effect of various sealers on the endodontologically detectable species enterococcus faecalis (e. faecalis), fusobacterium nucleatum (f. nucleatum) and porphyromonas gingivalis (p. gingivalis). | 2013 | 23523285 |
| in vitro activity of biapenem plus rpx7009, a carbapenem combined with a serine β-lactamase inhibitor, against anaerobic bacteria. | biapenem is a carbapenem being developed in combination with rpx7009, a new inhibitor of serine β-lactamases. biapenem was tested alone and in combination with fixed concentrations of rpx7009 by agar dilution against 377 recent isolates of anaerobes. a separate panel of 27 isolates of bacteroides spp. with decreased susceptibility or resistance to imipenem was also tested. comparator drugs included meropenem, piperacillin-tazobactam, ampicillin-sulbactam, cefoxitin, ceftazidime, metronidazole, c ... | 2013 | 23529731 |
| an investigation of the presence of specific anaerobic species in necrotic primary teeth. | different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. the aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (pcs) and root canals (rcs) of infected primary teeth using pcr methods. paired pc and rc samples were collected from 15 subjects and analyzed by pcr for the presence of filifactor alocis, fusobacterium nucleatum, parvimonas micra, porphyromonas endod ... | 2013 | 23538426 |
| host susceptibility to periodontitis: mapping murine genomic regions. | host susceptibility to periodontal infection is controlled by genetic factors. as a step toward identifying and cloning these factors, we generated an a/j x balb/cj f2 mouse resource population. a genome-wide search for quantitative trait loci (qtl) associated with periodontitis was performed. we aimed to quantify the phenotypic response of the progenies to periodontitis by microct analysis, to perform a genome-wide search for qtl associated with periodontitis, and, finally, to suggest candidate ... | 2013 | 23539559 |
| lemierre's syndrome: a rare case of pulmonic valve vegetation. | lemierre's syndrome is an uncommon complication of pharyngitis commonly associated with an anaerobic gram negative bacterium, fusobacterium necrophorum. the syndrome usually affects young healthy adults with the mean age of 20 and is characterized by recent pharyngitis followed by ipsilateral internal jugular vein thrombosis and septic thromboembolism. the treatment is at least 6 weeks of antibiotics; the role of anticoagulation is unclear. the following presentation is a case of lemierre's synd ... | 2013 | 23573433 |
| quantitative real-time pcr combined with propidium monoazide for the selective quantification of viable periodontal pathogens in an in vitro subgingival biofilm model. | differentiation of live and dead cells is an important challenge when using molecular diagnosis for microbial identification. this is particularly relevant when bacteria have been exposed to antimicrobial agents. the objective of this study was to test a method using quantitative real-time polymerase chain reaction (qpcr) combined with propidium monoazide (pma), developed for the selective quantification of viable p. gingivalis, a. actinomycetemcomitans, f. nucleatum and total bacteria in an in ... | 2014 | 23581569 |
| antimicrobial activity of ethanol extracts of laminaria japonica against oral microorganisms. | laminaria japonica is a brown alga, which is consumed widely in korea, japan, and china. this study investigated the antimicrobial activity of ethanol extracts of l. japonica against oral microbial species to assess the possible application of l. japonica extracts in dental care products. the minimal inhibitory concentration (mic) and minimal bactericidal concentration (mbc) were determined in culture medium using a microdilution method. the mics of ethanol extracts of l. japonica with oral stre ... | 2013 | 23583539 |
| advancement in the routine identification of anaerobic bacteria by maldi-tof mass spectrometry. | we evaluated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) biotyper as a tool for the identification of anaerobic bacteria compared with 500 base-pair (bp) 16s ribosomal ribonucleic acid (rrna) gene sequencing analysis, which is considered to be the "gold standard" method. a total of 484 anaerobic bacteria were retrieved from the clinical specimens of 318 pediatric patients. molecular identification resulted in 18 genera and 51 species. the most prev ... | 2013 | 23584672 |
| suppression of t-cell chemokines by porphyromonas gingivalis. | porphyromonas gingivalis is a major pathogen in periodontal disease and is associated with immune dysbiosis. in this study, we found that p. gingivalis did not induce the expression of the t-cell chemokine ip-10 (cxcl10) from neutrophils, peripheral blood mononuclear cells (pbmcs), or gingival epithelial cells. furthermore, p. gingivalis suppressed gamma interferon (ifn-γ)-stimulated release of ip-10, itac (cxcl11), and mig (cxcl9) from epithelial cells and inhibited ip-10 secretion in a mixed i ... | 2013 | 23589576 |
| microcalorimetric determination of the effects of amoxicillin, metronidazole, and their combination on in vitro biofilm. | the mechanism of action of adjuvant antibiotic therapy in the treatment of peri-implantitis is not well understood. the aim of this study is to investigate antibiotic susceptibility of an in vitro biofilm by isothermal microcalorimetry (imc). | 2014 | 23594193 |
| a case of adolescent pelvic inflammatory disease caused by a rare bacterium: fusobacterium nucleatum. | pelvic inflammatory disease (pid) is a polymicrobial infection that typically occurs in sexually active females. pid is rare in premenarchal and/or noncoital young women; however, there are case reports of pid occurring in virginal females. we present the first reported case of pid associated with f. nucleatum. in this unusual case of pid in an adolescent, the responsible organism may have originated from the patient's oral cavity. | 2013 | 23619431 |
| mobile microbiome: oral bacteria in extra-oral infections and inflammation. | the link between oral infections and adverse systemic conditions has attracted much attention in the research community. several mechanisms have been proposed, including spread of the oral infection due to transient bacteremia resulting in bacterial colonization in extra-oral sites, systemic injury by free toxins of oral pathogens, and systemic inflammation caused by soluble antigens of oral pathogens. mounting evidence supports a major role of the systemic spread of oral commensals and pathogen ... | 2013 | 23625375 |
| comparative evaluation of subgingival plaque microflora in pregnant and non-pregnant women: a clinical and microbiologic study. | gingival changes in pregnancy have been attributed to changes in the subgingival biofilm related to hormonal variations. | 2013 | 23633772 |
| evaluation of periodontal pathogens in amniotic fluid and the role of periodontal disease in pre-term birth and low birth weight. | pre-term birth and/or low birth weight (ptlbw) is a serious problem in developing countries. the absence of known risk factors in ≈ 50% of ptlbw cases has resulted in a continued search for other causes. the aim of this study was to examine the effect of periodontitis on pregnancy outcomes. | 2012 | 23638858 |
| the lipid bilayer-inserted membrane protein bama of escherichia coli facilitates insertion and folding of outer membrane protein a from its complex with skp. | folding of β-barrel membrane proteins, either from a urea-unfolded form or from chaperone-bound aqueous forms, has been characterized for pure lipid bilayers. the impact of preinserted integral proteins from biomembranes has not been examined in biophysical comparisons, but this knowledge is important for the characterization of protein assembly machinery in membranes to distinguish specific effects from unspecific effects. here, folding was studied for a β-barrel membrane protein, outer membran ... | 2013 | 23641708 |