Publications

identification of a unique lymphocyte subpopulation in the sheep uterus.	a panel of monoclonal antibodies was used to define the lymphocyte subpopulations in the sheep uterus at various stages of the oestrous cycle. a striking finding was that the majority of lymphocytes in the uterine and endometrial glandular epithelia belonged to a unique lymphocyte subpopulation that expressed the cd45r antigen but was negative for major histocompatibility complex (mhc) class ii molecules and expressed low or undetectable levels of the cd5 antigen. when examined under the electro ...	1988	2962930
chronic renal disease and hyperparathyroidism: dental manifestations and management.		2010	2963695
the pattern of atrial natriuretic peptide release during ventricular tachycardia in man.	1. plasma levels of atrial natriuretic peptide (anp) are high in many patients with tachycardia, but patterns of release with onset and termination of tachycardia and relationships to haemodynamic recordings are not clear. blood for anp measurements was therefore drawn from the coronary sinus, femoral artery and femoral vein, and simultaneous haemodynamic recordings were made in five patients before, during and after induction of stable ventricular tachycardia for 30 min. 2. tachycardia induced ...	1987	2963719
high affinity aldosterone binding sites (type i receptors) in rat heart.	1. the use of the receptor stabilizing agent sodium molybdate, and of ru26988 to exclude [3h]-aldosterone binding from type ii glucocorticoid receptors, has enabled the characterization of high affinity type i aldosterone binding sites in rat atrial and ventricular cytosols. 2. in adult male and female rats the affinity of binding (kd 4 degrees c) is approximately 1-2 nmol/l for both atria and ventricles; specificity of binding is similar to that for type i sites in classical aldosterone target ...	1987	2966024
[pulmonary sarcoidosis with bullous development].		2010	2966391
[use of physostigmine in anesthesiology].		2010	2966440
an antitumor polypeptide antibiotic neocarzinostatin: the mode of apo-protein--chromophore interaction.	the mode of neocarzinostatin-chromophore (ncs-chr)-apo-neocarzinostatin (apo-ncs) interaction in neocarzinostatin (ncs) complex has been described. the ncs-chr release from the ncs complex in the presence of various reagents, which destroy the high-order structure of the protein under various ph conditions, was examined. we found that (i) sodium dodecylsulfate, nonidet p 40, 8 m urea, and 2-propanol did release ncs-chr from ncs, (ii) no ncs-chr release is detected below ph 7, but it is enhanced ...	2010	2967272
a proposed mechanism of action for alg and atg in severe acquired aplastic anaemia.	in this study we have shown that various batches of anti-lymphocyte globulin (alg), anti-thymocyte globulin (atg) and human intravenous immunoglobulin (iv ig) all contain antibodies with the capacity to block lymphocyte receptors for the fc region of igg, i.e., fc gamma receptors. these antibodies may exert an effect on fc gamma receptor bearing suppressor t cell function in vivo. since t cells have been implicated in the pathogenesis of acquired severe aplastic anaemia, it is conceivable that a ...	1988	2967377
intestinal bacterial flora and host defense mechanisms.	the protective mechanisms of intestinal bacterial flora against exogenous infections will be discussed in this paper. experimental data on protective function of escherichia coli, enterococcus faecalis and bacteroides distasonis comprising intestinal flora against oral infection of shigella flexneri which causes localized infection are presented. furthermore, the present investigation deals with non-specific defense mechanism which indicate that protective activity of intestinal flora against pa ...	1986	2968005
genetics and regulation of enterobactin genes in shigella flexneri.	although shigella flexneri possesses the genes for two siderophore systems, enterobactin and aerobactin, the enterobactin system is only rarely utilized. to investigate the regulation of enterobactin expression in s. flexneri, all of the genes specifically required for synthesis and transport of enterobactin were cloned from both an expressing (ent+) and a nonexpressing (ent-) strain. notable differences between the cloned genes included endonuclease restriction site changes and the presence of ...	1988	2973458
[clinical characteristics of dysentery today].		1988	2973539
[fibrinolytic therapy of thromboembolic vascular occlusions: experiences based on 87 patients].		2010	2974192
multiple gamma radiation sterilization of polyester fibres.	gamma radiation with a dose of 2.5 mrad has been found to be suitable to sterilize polyethylene terephthalate (pet) bulk materials intended for biomedical applications. the radiation stability of pet bulk materials and fibre may not however be taken as identical due to the changes in the polymer structure during the processing of bulk materials for fibre. the chemical changes occurring in pet fibres during single and multiple (prolonged) sterilization in air were investigated. it was found that ...	2010	2975186
dental implications of pharmacological management of the alzheimer's patient.		2010	2975266
[application of ptca steerable guide wire for abdominal and peripheral interventional angiography].		2010	2975374
myosin atpase activity during avian cardiac and skeletal muscle development.	the myosin atpase activity and myosin light chain composition in developing chick heart and skeletal muscles were studied and compared. embryonic myosin was purified and characterized from day 7 to day 19 of embryogenesis. embryonic cardiac myosin generally showed the same ca2+-activated myosin atpase activity level as the adult value. in comparison, pooled pectoralis and hindlimb skeletal muscles from day 10 through day 19 showed myosin atpase activities that were all significantly less than th ...	1988	2976104
apparent hyperalgesia in the mouse tail-flick test due to increased tail skin temperature after lesioning of serotonergic pathways.	the relationship between tail skin temperature and responsiveness to noxious radiant heat in the tail-flick test was investigated in mice. a significant negative correlation between tail skin temperature and tail-flick latency was found when the tail skin temperature was increased by elevating the ambient temperature. after intracerebroventricular injection of the serotonin neurotoxin 5,7-dihydroxytryptamine (5,7-dht, 80 micrograms) tail skin temperatures were increased and tail-flick latencies ...	1988	2976242
[studies on attenuation of cardiovascular responses during endotracheal intubation following rapid-sequence induction of anesthesia].		2010	2976320
hyperresponsiveness to water immersion in sodium retaining cirrhotics: the role of atrial natriuretic factor.	cirrhotics do not respond uniformly to head-out water immersion (hwi). some cirrhotics have an exaggerated natriuresis while others are unresponsive. we studied the humoral and urinary responses to hwi in 5 cirrhotics and compared this to 5 normals on the same 20 mm sodium intake. these cirrhotics had a documented tendency to salt and water retention but had minimal or no evidence of significant ascites. plasma levels of atrial natriuretic factor (anf) increased during immersion in both groups. ...	2010	2976331
ex vivo platelet studies following oral nisoldipine in normotensive insulin-dependent diabetics and non-diabetic controls.	the effect of 24 hours and 7 days treatment with nisoldipine (10 mg, twice daily) on platelet function was studied in 12 normotensive volunteers of whom six were insulin-dependent diabetics without clinical evidence of vascular complications. platelet aggregation was assessed by platelet rich plasma (prp) and whole blood (wb) techniques. in addition, the effect of nisoldipine on platelet hyperaggregability following exercise was assessed. after taking nisoldipine for 24 hours, in vitro platelet ...	2010	2976336
[changes in metabolic control after interruption of oral hypoglycemic agents in type ii diabetic patients with secondary failure during combined therapy].		2010	2976347
multiple small papular seborrheic keratosis.		2010	2976363
technique for measurement of one-dimensional instantaneous ablation velocity.	an oscillating mirror scanned a he-ne laser beam along the length of a thin rod while the rod was ablated by an argon laser beam. the shadow of the scanning beam on an image plane provides a picture of the instantaneous ablation velocity of the rod. this configuration provides a unique method for investigating one-dimensional ablation.	2010	2976448
[rpo pathway as a possible recombination mechanism of the interaction between dna of transducing phages and the escherichia coli genome].	the conditions affecting recombination of dnas of transducing lambdoid rifd phages with the chromosome of escherichia coli k-12 reca in the region of homology were studied. in support to the previously obtained data, the int system was shown to take no part in the process. the homologous character of recombination interactions and their dependence on efficiency of transcription were demonstrated. it is therefore suggested that recombination takes the rpo pathway. similar peculiarities were revea ...	1988	2976700
hypersensitivity to shigella outer membrane proteins in mice.	mice sensitized with living sh. flexneri 3a or with outer membrane proteins (omp) derived from these bacteria showed arthus and delayed type hypersensitivity (dth) when injected in the footpad with omp. the delayed reactivity to omp was transferable to normal mice by spleen cells obtained from donor animals which were previously sensitized with living bacteria.	1987	2977277
[the possibility of using the demonstration of shigella antigens in the study of the epidemic process in dysentery].	the detection of shigella antigens in feces in accordance with the results of the passive hemagglutination test is more effective than the bacteriological method for finding out persons infected with shigella, especially in the absence of pronounced clinical manifestations of dysentery, and can be used in the study of the mechanism of the epidemic process. the examination of two groups, each consisting of permanent and temporary members, has revealed that the use of this method for detection of ...	1988	2977464
[the results of a seroepidemiologic study of newcastle dysentery].	differences in the monthly distribution in the number of seropositive individuals among children and adults in years with different intensity of the epidemic process have been revealed. immunity in cases of dysentery caused by s. newcastle reflects the yearly and seasonal activation of the epidemic process in this shigella infection both in children and adults, the seroconversion characteristics observed in the year of a high morbidity level being a more objective criterion indicating the beginn ...	1988	2977465
[experimental study of atherosclerosis. i. anti-atherosclerotic actions of chinese herb effective component 8501].		1988	2978250
recurrence of hepatic amebiasis after successful treatment with metronidazole.	a patient with an amebic liver abscess had an incomplete response to an initial course of metronidazole, but subsequently responded to a prolonged course of the agent. recurrence of disease in the contralateral hepatic lobe responded to chloroquine along with a luminicide. we took the initial failure of metronidazole to be secondary to either the advanced nature of the disease or the development of partial resistance to metronidazole. the recurrence was the result of reinfection of the liver by ...	2008	2980773
characterization of a major protein with a molecular weight of 160,000 associated with the viral capsid of epstein-barr virus.	a monoclonal antibody designated v3 was produced against a late protein associated with the epstein-barr virus-induced viral capsid antigen complex. the antibody reacted with discrete patches in the nuclei of infected cells as well as with virus particles, as shown by immunofluorescence and ultrastructural immunoperoxidase staining. the molecular weight of the protein precipitated by this monoclonal antibody was ca. 160,000. all anti-viral capsid antigen antibody-positive sera tested in an enzym ...	1985	2981328
middle tumor antigen of polyomavirus transformation-defective mutant ng59 is associated with pp60c-src.	we have found that lysis of mouse embryo cells infected with the polyomavirus host range transformation-defective (hr-t) mutant ng59 under gentle conditions that avoid ionic detergents results in detectable ng59-encoded middle tumor antigen (mtag) associated with pp60c-src. this mtag-pp60c-src complex could be immunoprecipitated from ng59-infected cell lysates by either sera from animals bearing polyomavirus-induced tumors or by monoclonal antibodies directed against mtag. immune complex kinase ...	1985	2981329
genome organization of herpesvirus aotus type 2.	herpesvirus aotus type 2, a virus commonly found in owl monkeys without overt disease, has a similar genome structure to the oncogenic herpesviruses of nonhuman primates (herpesvirus saimiri, herpesvirus ateles). virion dna of herpesvirus aotus type 2 (m-dna) has an unique 110-kilobase-pair region of low g + c content (40.2%, l-dna), inserted between stretches of repetitive h-dna (68.7% g + c, about 41 kilobase pairs per molecule) that are variable in length. a minority of virions contain defect ...	1985	2981331
autoregulation of the bacteriophage p22 scaffolding protein gene.	during the formation of each bacteriophage p22 head, about 250 molecules of the product of gene 8, scaffolding protein, coassemble with and dictate correct assembly of the coat protein into a proper shell structure. at approximately the time that dna is inserted inside the coat protein shell, all of the scaffolding protein molecules leave the structure. they remain active and participate in several subsequent rounds of shell assembly. previous work has shown that scaffolding protein gene express ...	1985	2981337
analysis of mobilization elements in plasmids from shigella flexneri.	the mobilization properties of three plasmids were examined after cotransfer from shigella flexneri to escherichia coli. the largest plasmid, pcn1, was shown to be a conjugative r factor that could promote its own transfer and allow cotransfer of a 4.1-kilobase plasmid, pcn3; mobilization of the third plasmid, pcn2 (6.3 kilobases), required the presence of both pcn1 and pcn3. sequences from pcn2 and pcn3 homologous to the bom (basis of mobilization) sites of cole1 and pbr322 were localized by an ...	1985	2982788
adenine nucleotides directly stimulate pertussis toxin.	both cholera toxin and pertussis toxin catalyzed adp-ribosylation of purified bovine brain tubulin. the effect of cholera toxin was evident in the absence or presence of nucleotides. in contrast, pertussis toxin required adenine nucleotides for its adp-ribosylating activity. atp, atp gamma s, app(nh)p, deoxy-atp, and adp all supported pertussis toxin-catalyzed adp-ribosylations in the absence or presence of edta, suggesting that nucleotide hydrolysis was not involved. adenine nucleotides also pr ...	1985	2982826
a new beta-lactamase plasmid in neisseria gonorrhoeae.		1985	2984155
peptide-nucleic acid interactions: possible recognition determinants of eco ri endonuclease.		2008	2986169
peptide-nucleic acid interactions: possible recognition determinants of eco ri endonuclease.		2008	2986169
escherichia coli 23s ribosomal rna truncated at its 5' terminus.	in a strain of e. coli deficient in rnase iii (abl1), 23s rrna has been shown to be present in incompletely processed form with extra nucleotides at both the 5' and 3' ends (king et al., 1984, proc. natl. acad. sci. u.s. 81, 185-188). rna molecules with four different termini at the 5' end are observed in vivo, and are all found in polysomes. the shortest of these ("c3") is four nucleotides shorter than the accepted mature terminus. in growing cells of both wild-type and mutant strains up to 10% ...	1985	2987818
opioid modulation of ingestive behaviors in woodchucks and racoons.	we have examined the effect on feeding of opioid blockade with naloxone in two species which demonstrate a marked seasonality in their feeding patterns, the racoon (procyon lotor) and the woodchuck (marmota monax). naloxone suppressed food intake in the woodchuck which is a true hibernator. naloxone failed to suppress food intake in the racoon and, in fact, enhanced intake of a preferred sucrose solution. in the racoon, ir-dynorphine concentrations were extremely high in the hypothalamus compare ...	1985	2987986
isolation of retinoic acid-resistant clones from human breast cancer cell line mcf-7 with altered activity of cellular retinoic acid-binding protein.	after ethyl methane sulfonate mutagenesis of the mammary carcinoma cell line, mcf-7, we have isolated three clones, u-2, u-3, and u-9, resistant to retinoic acid. these three clones showed more than a 1000-fold higher level of resistance to retinoic acid than the parental mcf-7 cells when assayed by colony formation in monolayer culture system or by growth curves. the three resistant clones showed a 200-fold higher resistance to 13-cis-retinoic acid, about 10-fold higher resistance to retinol, a ...	1985	2988768
mercuric reductase structural genes from plasmid r100 and transposon tn501: functional domains of the enzyme.	the nucleotide sequence for the 2240 bp of plasmid r100 following the merc gene of the mercuric resistance operon has been determined and compared with the homologous sequence of transposon tn501. the sequences following merc and preceding the next structural gene mera are unrelated between r100 and tn501 and differ in length, with 72 bp in tn501 and 509 bp in r100. the r100 sequence has a potential open reading frame (orf) for a 140 amino acid polypeptide with a reasonable translational start s ...	1985	2989109
cloning of plasmid dna sequences involved in invasion of hela cells by shigella flexneri.	a large plasmid is found in virulent isolates of shigella sp. and encodes functions essential for invasion of mammalian cells. to identify plasmid sequences necessary for invasion, we isolated a series of tn5 insertions in pwr100, the virulence plasmid of shigella flexneri serotype 5. these insertions demonstrated that three separate ecori fragments of pwr100 were required for invasion of hela cells. however, the corresponding native ecori fragments, when cloned into pbr325, did not restore viru ...	1985	2989179
metastasis of an occult gastric carcinoma suggesting growth of a prolactinoma during bromocriptine therapy: a case report with a review of the literature.	the treatment of a slowly growing invasive prolactinoma with bromocriptine for 8 months resulted in a substantial decrease in plasma prolactin levels despite rapid suprasellar tumor expansion. on exploration, this uncommon observation could be attributed to hematogenous metastasis from an occult gastric adenocarcinoma to the pituitary tumor. apart from infiltration of neighboring parts of the hypothalamus, autopsy revealed no other hematogenous metastases. this extraordinary type of neoplasm-to- ...	2010	2989726
inhibition and activation of bovine heart nad-specific isocitrate dehydrogenase by atp.	in the absence of added calcium, inhibition of nad-specific isocitrate dehydrogenase by atp occurred without adp (i0.5 = 1.8 mm) and with 0.2 mm adp3- (i0.5 = 1.0 mm) at subsaturating substrate concentrations at ph 7.4. inhibition by atp was competitive with nad+ in the presence and absence of adp and was not reversed by magnesium citrate. no reversal of atp inhibition by free ca2+ was observed in the presence of adp (0.2 mm). however, when adp was absent, increasing ca2+ first caused progressiv ...	1985	2990340
bowenoid papulosis. presence of human papillomavirus (hpv) structural antigens and of hpv 16-related dna sequences.	this study reviews 39 cases of anogenital bowenoid papulosis lesions in 22 individuals of both sexes that were analyzed clinically, histologically, immunocytochemically, and virologically. macroscopically, three different types of lesions were demonstrated: erythematous macules; papules (lichenoid and/or pigmented papules); and leukoplakialike lesions. microscopically, bowenoid papulosis fulfills the criteria of a squamous cell carcinoma in situ. much like oral precancers, three distinct growth ...	1985	2990353
stimulation of oil biodegradation by using slow-release fertilizers.		2008	2991040
[survival of aujeszky's disease virus in infected pig slurry].	it has been demonstrated that after experimental infection of pig slurry from the space under the slatted floor (infection dose of 10(6)pfu per ml), the aujeszky's disease virus (adv) survived for 72 hours at the temperature of 15 degrees c and at ph 6.5, but was inactivated after 96 hours. when technologically treated pig slurry from the storage tanks was saturated with water and infected with adv at the dose of 10(5)pfu per ml, the virus survived for 23 days when kept at 15 degrees c and 4 deg ...	1985	2992149
rates of cholesterol synthesis and low-density lipoprotein uptake in the adrenal glands of the rat, hamster and rabbit in vivo.	the absolute rate of cholesterol acquisition from de novo synthesis and from receptor-dependent and receptor-independent low-density lipoprotein (ldl) uptake was determined in the adrenal glands of the rat, hamster and rabbit under in vivo conditions. the rate of incorporation of [3h]water into cholesterol in the adrenal gland was much higher in the hamster (1727 nmol/h per g) and rabbit (853 nmol/h per g) than in the rat (71 nmol/h per g). assuming that 23 atoms of 3h are incorporated into the ...	1985	2992599
suicide vector for transposon mutagenesis in pseudomonas solanacearum.	a suicide vector was constructed by cloning the transfer genes of the wide-host-range (incw group) plasmid r388 into the bamhi site of pbr325. this plasmid can deliver tn5 into pseudomonas solanacearum at frequencies ranging from 10(-6) to 10(-9) per recipient.	1985	2993241
amino acid sequence of acylphosphatase from porcine skeletal muscle.	the amino acid sequence of acylphosphatase from porcine skeletal muscle was determined. it consists of 98 amino acid residues with n-acetylserine at the amino (n)-terminus: ac-ser-thr-ala-arg-pro-leu-lys-ser-val-asp-tyr-glu-val-phe-gly -arg-val-gln-gly-val-cys-phe-arg-met-tyr-thr-glu-asp-glu-ala-arg-lys-ile -gly-val-val-gly-trp-val-lys-asn-thr-ser-lys-gly-thr-val-thr-gly-gln -val-gln-gly-pro-glu-glu-lys-val-asn-ser-met-lys-ser-trp-leu-ser-lys -ile-gly-ser-pro-ser-ser-arg-ile-asp-arg-thr-asn-phe- ...	1985	2993259
the biologic significance of the mixed lymphocyte kidney culture in humans.	the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ...	1985	2996186
[the virus causing human t-cell lymphomas-leukemias].		1985	2996236
the e7-associated cell-surface antigen: a marker for the 11p13 chromosomal deletion associated with aniridia-wilms tumor.	unbalanced interstitial deletions of the p13 region of human chromosome 11 have been associated with congenital hypoplasia or aplasia of the iris, mental retardation, ambiguous genitalia, and predisposition to wilms tumor of the kidney. utilizing somatic cell hybrids containing either the normal or abnormal chromosome 11 from a child with wilms tumor and aniridia, we previously mapped the e7 cell-surface antigen to the 11p1300-to-11p15.1 region. to localize even further the site of this antigen ...	1985	2996335
[current trends in the development of the science of coagulation abroad].		2010	2996966
acquired immune deficiency syndrome. a perspective for the medical practitioner.	aids is a new disease process with complications and management problems unlike anything ever seen before. an attempt has been made to summarize the available information about its various clinical presentations and how to manage them. subjects covered will include associated malignancies, infections, transmissibility, prodromal states, and infection control issues.	1985	2997554
pyrimidinones. 1. 2-amino-5-halo-6-aryl-4(3h)-pyrimidinones. interferon-inducing antiviral agents.	interferon induction and antiviral activity was discovered with 2-amino-5-bromo-6-phenyl-4(3h)-pyrimidinone. an analogue study incorporating a series of 2-amino-5-substituted-6-arylpyrimidinones revealed that the most potent interferon inducers were mono- and difluorophenyl analogues. these same analogues were also potent antiviral agents against semliki forest virus and herpes simplex type 1. in addition the monomethoxyphenyl analogues were potent antiviral agents but weak interferon inducers. ...	1985	2999405
cutaneous histiocytosis syndromes.	cutaneous histiocytosis may take two principal forms. it is either a benign proliferative process or a relentless, progressive process with a poor prognosis. in histiocytic medullary reticulosis, histiocytes demonstrate nuclear atypia and the outcome is uniformly fatal. benign cephalic histiocytosis x causes lesions similar to those of histiocytosis x, but langerhans' cells are absent. in congenital self-healing histiocytosis x, the letterer-siwe-like cutaneous infiltrate contains langerhans' ce ...	2009	2999740
elicitation of conspecific attack or defense in the male rat by intraventricular injection of a gaba agonist or antagonist.	the involvement of central gabaergic mechanisms in the control over offensive and defensive behaviours in the rat was studied using intracerebroventricular injections (5 microliter) of a gaba agonist (thip) or a gaba antagonist (bicuculline methiodide). intracerebroventricular injections of thip (1.25 and 2.5 micrograms) induced attacks and offensive sideways towards an untreated partner, in animals placed in a neutral area where no aggressive reactions occur in controls. social approach behavio ...	1985	2999844
elicitation of conspecific attack or defense in the male rat by intraventricular injection of a gaba agonist or antagonist.	the involvement of central gabaergic mechanisms in the control over offensive and defensive behaviours in the rat was studied using intracerebroventricular injections (5 microliter) of a gaba agonist (thip) or a gaba antagonist (bicuculline methiodide). intracerebroventricular injections of thip (1.25 and 2.5 micrograms) induced attacks and offensive sideways towards an untreated partner, in animals placed in a neutral area where no aggressive reactions occur in controls. social approach behavio ...	1985	2999844
role of leukotrienes in rat reversed passive arthus pleurisy and the effect of aa-861, a 5-lipoxygenase inhibitor.	in studies of the role of leukotrienes in inflammatory reactions, the induction of rat reversed passive arthus pleurisy (a type iii allergic reaction) was employed. increases of exudate volume, vascular permeability, and migration of inflammatory cells in the pleural cavity were observed. the vascular permeability was enhanced biphasically during 0-30 min (early response) and during 3-6 h (late response) after induction of the pleurisy. the infiltration of inflammatory cells, mainly polymorphonu ...	1986	3000949
flaviviridae.	the family flaviviridae comprises the genus flavivirus, which contains 65 related species and two possible members. they are small, enveloped rna viruses (diameter 45 nm) with peplomers comprising a single glycoprotein e. other structural proteins are designated c (core) and m (membrane-like). the single strand of rna is infectious and has a molecular weight of about 4 x 10(6) and an m7g 'cap' at the 5' end but no poly(a) tract at the 3' end; it functions as the sole messenger. the gene sequence ...	1985	3000978
repression of adenovirus early gene expression by coinfection with a temperature-sensitive mutant in the immediate-early gene of pseudorabies virus.	wild-type adenovirus was coinfected with a mutant temperature sensitive for the immediate-early gene of pseudorabies virus. at the nonpermissive temperature, this mutant, tsg, strongly inhibited the transcription of all adenovirus early genes, including e1a. this inhibition was not observed with wild-type pseudorabies virus coinfection or with tsg coinfection at the permissive temperature. the level of repression was dependent upon the ratio of tsg to adenovirus in the infection. the results sug ...	1986	3001339
relationship of acth1-39-immunostained fibers and magnocellular neurons in the paraventricular nucleus of rat hypothalamus.	dual antigen immunocytochemical staining procedures were used in the same tissue section to determine the distribution of acth immunostained fibers and varicosities within the magnocellular and parvocellular divisions in the paraventricular nucleus (pvn) of rat hypothalamus and elucidate its anatomical relationship to vasopressin (vp) and oxytocin (oxy)-containing neurons. double immunostained preparations using glucose oxidase-antiglucose oxidase complex combined with pap complex to visualize t ...	1985	3001668
characterization of electron transfer and proton translocation activities in trypsin-treated bovine heart mitochondrial cytochrome c oxidase.	bovine heart mitochondrial cytochrome c oxidase has been treated with trypsin in order to investigate the role of components a, b, and c (nomenclature of capaldi) in cytochrome c binding, electron transfer, and proton-pumping activities. cytochrome c oxidase was dispersed in nondenaturing detergent solution (b. ludwig, n. w. downer, and r. a. capaldi (1979) biochemistry 18, 1401) and treated with trypsin. this treatment inhibited electron transfer activity by 9% when compared to a similarly trea ...	1985	3002279
molecular alteration of the 140-megadalton plasmid associated with loss of virulence and congo red binding activity in shigella flexneri.	a plasmid of about 140 megadaltons has been associated with the invasiveness of shigella flexneri. upon subculturing in liquid media of fully virulent isolates of shigella flexneri 2a ysh6000, which contains only a 230-kilobase-pair (kbp) plasmid in addition to 3.3- and 4.2-kbp cryptic plasmids characteristic to all s. flexneri strains, loss of invasiveness, loss of congo red binding activity (pcr), and complete loss of, or a deletion, or even a single-site is insertion in the plasmid occurred s ...	1986	3002985
bronchiolar and alveolar lesions in the pathogenesis of crocidolite-induced pulmonary fibrosis in mice.	asbestosis is generally considered to result in restrictive pulmonary disease associated with interstitial fibrosis. recently, however, attention has focused upon bronchiolar lesions and concomitant obstruction to air flow. the responses of conducting airways and alveoli were studied over a 20 week period following instillation of crocidolite to mice. the location of the lesions and the sequential inflammatory changes were studied by bronchoalveolar lavage, light and electron microscopy; regener ...	1985	3003309
[the na,k pump, the cellular receptor for digitalis glycosides].		2010	3003996
[the kallikrein-kinin system in acute peritonitis and methods of correcting its disorders].	in experimental fecal peritonitis in 140 albino rats the kallikrein-kinin system was shown to undergo the following changes: its inhibition during the first day was followed by an activation on the second day and inanition on the third day. under conditions of hyperbaric oxygenation the normalizing effect on the kinin system was observed during two days. the application of salicylates against the background of hbo was shown to enhance the normalizing effect which lasted for three days.	1985	3004000
fluctuation of specific iga antibodies in human milk.	the concentration of secretory iga and the levels of iga specific antibodies against escherichia coli labile-toxin, shigella flexneri 6, and rotaviruses were determined in milk samples obtained serially from women during the first 16 weeks postpartum. the mean concentration of secretory iga followed the expected pattern; the levels of specific antibodies fluctuated in an unpredictable manner and independently of milk secretory iga content, becoming undetectable in many instances. under some circ ...	1985	3004100
hormonal control of polyamine levels in bovine adrenocortical cells.	the implication of polyamines in cellular growth and differentiation processes and the existence of a polyamine-mediated protein phosphorylation system in adrenocortical cells suggest that polyamines may be examined as potential intracellular messengers in the pleiotypic action of acth. bovine adrenocortical cells in culture exhibit a specific, energy-dependent, partly sodium-supported, inward polyamine transport system, independent of the a, l, and n aminoacid uptake systems. steroidogenic conc ...	1986	3004891
studies on the glycoprotein nature of the thyrotropin receptor: interaction with lectins and purification of the bovine protein with the use of bandeiraea (griffonia) simplicifolia i affinity chromatography.	the tsh receptor from triton-solubilized bovine microsomal membranes was found to bind to a substantial extent to columns of the immobilized lectins bandeiraea (griffonia) simplicifolia i, ricinus communis i, wheat germ, and concanavalin a, whereas it was not retained by dolichos biflorus. elution of tsh receptor activity from these lectins could be achieved with the appropriate saccharides in all cases except concanavalin a. the most extensive adsorption of the receptor occurred on b. simplicif ...	1986	3004903
[similar organization of beta,beta'-subunit rna-polymerase genes and adjacent ribosomal protein genes in enterobacteriaceae and pseudomonas putida].	the genes coding for the rna-polymerase beta,beta'-subunits and adjacent ribosomal protein genes in escherichia coli, salmonella typhimurium, shigella flexneri, serratia marcescens, proteus mirabilis and pseudomonas putida are compared by the southern hybridization procedure. in all the species studied close clustering of the genes rplkajl and rpobc is demonstrated. preliminary physical maps for these genes in s. typhimurium, s. flexneri, s. marcescens and p. mirabilis are proposed. rifampicin i ...	1986	3005845
enteropathogens associated with acute diarrhea in hospitalized infants.	thirty-five infants of low socioeconomic status who were living in urban santiago were hospitalized for acute diarrhea were prospectively evaluated for the presence of enteropathogens associated with the episode. some degree of malnutrition was evident in 20 infants (57.1%); 15 of these (75%) were under 6 months of age. mean duration of the hospital stay was 11.8 days for well-nourished patients and 15.7 days for the malnourished patients. one or more enteropathogens were found in 60% of the cas ...	1986	3007718
interest of the dosage of the h.c.g. in the spermatical blood at the moment of the castration for a testicular tumor (about 28 cases 1978-1984).		2010	3008177
[computed tomographic determination of thyroid iodine concentration in an endemic goiter area].	we determined the ct density of the thyroid gland in 60 patients with normal thyroids and 176 patients with various thyroid disorders. the density was 75 +/- 6.2 h.u. in normal thyroids, which was markedly higher than in goiters (66 +/- 6.0 h.u.), while there was a considerable further decrease in patients with immunogenic hyperthyroidism, the density being 48.5 +/- 7.9 h.u. the thyroids of patients with nonimmunogenic hyperthyroidism differed from these by virtue of a significantly greater dens ...	2010	3008254
complete dissociation between the activation of phosphoinositide turnover and of nadph oxidase by formyl-methionyl-leucyl-phenylalanine in human neutrophils depleted of ca2+ and primed by subthreshold doses of phorbol 12,myristate 13,acetate.	evidences have been provided by many laboratories that the activation of the nadph oxidase in neutrophils by formyl-methionyl-leucyl-phenylalanine (fmlp) is strictly linked to a transduction pathway that involves the stimulation, via gtp binding protein, of the phosphoinositide turnover and the increase in [ca2+]i. the results presented in this paper demonstrate that fmlp can activate the nadph oxidase by triggering a transduction pathway completely independent of phosphoinositide turnover and c ...	1986	3008746
identification by antibody to a synthetic peptide of a protein specified by a diploid gene located in the terminal repeats of the l component of herpes simplex virus genome.	in the course of studies on the a sequences located at the termini of and at the junction between the l and s components of herpes simplex virus 1 dna, j. chou and b. roizman (j. virol. 57:629-637, 1986) noted that the a sequence acted as a gamma 1 promoter when fused to the structural sequence of the thymidine kinase gene, the b inverted repeat sequences located in the l component next to the a sequences contained an open reading frame predicted to encode the protein of 358 amino acids with a m ...	1986	3009891
the adenosine triphosphate-pyrophosphate isotopic exchange reaction: a tool for determination of tryptophan.	quantitative determination of tryptophan at the picomole level is described, using the atp-[32p]ppi isotopic exchange reaction catalyzed by tryptophanyl-trna synthetase. sensitivity limits of 500 fmol were obtained. the presence of other amino acids at a 1000-fold excess over tryptophan did not interfere significantly with the quantitative determination of tryptophan. the specificity of the reaction was checked using five tryptophan analogs. these analogs did not prevent the determination of try ...	2010	3014918
radioreceptor assay of anti-tsh receptor antibody activity: comparison of assays using unextracted serum and immunoglobulin fractions, and standardization of expression of activities.	a radioreceptor assay for tsh receptor antibodies developed by shewring and smith is described in which unextracted serum is used. the assay is simple and reproducible. tsh-binding inhibitor immunoglobulin (tbii) activity determined using unextracted serum correlated well with that determined using the immunoglobulin fraction purified with polyethylene glycol. the assay detected tsh receptor antibodies in 81 of 85 patients (95%) with untreated graves' disease, no patients with graves' disease in ...	1986	3016274
anti-htlv-iii screening of blood donors.	in a survey on 82,383 blood donor specimens from hessen, west germany, the observed percentage of repeatedly positive htlv-iii/lav antibody elisa results was 0.2%, but only 0.018% were confirmed by western blot, immunofluorescence assay or radioimmunoprecipitation assay. these data are consistent with larger epidemiological data compiled from other german blood banks and suggest a high degree of nonspecific false-positive results; one of the causes for false-positives are hla-dr4 antibodies, whi ...	1986	3017001
effects of theophylline and camp on amphiuma small intestine.		1986	3017122
effect of age on the formation of cyclic nucleotides in guinea-pig tracheal smooth muscle in response to pharmacological agents.	the effects of isoproterenol, carbachol and other drugs on the cyclic amp and cyclic gmp levels in tracheal smooth muscles of guinea-pigs of four different ages were investigated. isoproterenol increased the cyclic amp level several-fold in tracheal muscles from newborn (1 week) and young (4-7 weeks) guinea-pigs, but it caused less increase in the level in muscles from middle-aged (12 weeks) and old (20-24 weeks) guinea-pigs, although the basal cyclic amp level at these ages was not significantl ...	1986	3017724
steroid-responsive bronchiolitis after human heart-lung transplantation.	this report describes the clinical course of a patient who developed obliterative bronchiolitis after viral infection on three separate occasions. long-term follow-up is given. it is suggested that the syndrome of late pulmonary deterioration after transplantation may be steroid responsive if treatment is initiated early in the natural history of the syndrome. in addition, it is suggested that increased emphasis should be placed on the documentation of viral infection in transplant recipients to ...	1986	3018383
testing for htlv-iii/lav antibody.		1986	3019171
studies on the antihypertensive effect of single doses of the angiotensin converting enzyme inhibitor ramipril (hoe 498) in man.	the time course of the blood pressure lowering effect and the dose-response relationship of the new angiotensin converting enzyme inhibitor ramipril (hoe 498) were studied in 8 patients, with essential hypertension. as compared with placebo, a single oral dose of 2.5 mg ramipril lowered systolic and diastolic blood pressure. the antihypertensive action of single oral doses of 5, 7.5 and 10 mg ramipril was more pronounced. no change in heart rate occurred. angiotensin converting enzyme activity w ...	1986	3019702
studies on the antihypertensive effect of single doses of the angiotensin converting enzyme inhibitor ramipril (hoe 498) in man.	the time course of the blood pressure lowering effect and the dose-response relationship of the new angiotensin converting enzyme inhibitor ramipril (hoe 498) were studied in 8 patients, with essential hypertension. as compared with placebo, a single oral dose of 2.5 mg ramipril lowered systolic and diastolic blood pressure. the antihypertensive action of single oral doses of 5, 7.5 and 10 mg ramipril was more pronounced. no change in heart rate occurred. angiotensin converting enzyme activity w ...	1986	3019702
animal models for retrovirus-induced immunodeficiency disease.		1986	3019879
animal models for retrovirus-induced immunodeficiency disease.		1986	3019879
isolation, characterization, and mapping of tn5 insertions into the 140-megadalton invasion plasmid defective in the mouse sereny test in shigella flexneri 2a.	using shigella flexneri 2a ysh6000, we isolated 304 independent tn5 insertion mutants in the 230-kilobase invasion plasmid, pmysh6000. the site of each tn5 insertion was assigned to 23 sali fragments on the previously made sali cleavage map of pmysh6000. among the 304 insertions, 150 were negative in expression of four phenotypes examined (mouse sereny test [ser], invasion into epithelial cells [inv], congo red binding [pcr], and inhibition of bacterial growth [igr] ): 12 were ser- inv+ pcr+ igr ...	1986	3019891
immortalization of human t lymphocytes by htlv-i: phenotypic characteristics of target cells and kinetics of virus integration and expression.	in-vitro infection of normal human lymphocytes with htlv-i (human t-cell lymphotropic retrovirus type i) has been carried out to study the target cell specificity and the kinetics of infection. cord blood (cbl) and adult peripheral blood lymphocytes (pbl) have been co-cultivated with irradiated htlv-i donor cells (mt2 and c91pl lines). established ('immortalized') htlv-i positive cell lines were obtained only from cbl: in comparison with pbl, a less mature phenotype of t-cell subsets and a lower ...	1986	3020320
substrate phosphoprotein availability regulates eclosion hormone sensitivity in an insect cns.	the final step in the moulting of all insects is ecdysis, the shedding of the cuticle of the previous instar, which is triggered in lepidoptera by the neurosecretory peptide eclosion hormone. this hormone acts directly on the nervous system to release the appropriate motor patterns for larval, pupal and adult ecdysis, but there are only brief periods near the end of each moult when the nervous system is competent to respond to the hormone. previous experiments have shown that the action of eclos ...	1986	3020427
substrate phosphoprotein availability regulates eclosion hormone sensitivity in an insect cns.	the final step in the moulting of all insects is ecdysis, the shedding of the cuticle of the previous instar, which is triggered in lepidoptera by the neurosecretory peptide eclosion hormone. this hormone acts directly on the nervous system to release the appropriate motor patterns for larval, pupal and adult ecdysis, but there are only brief periods near the end of each moult when the nervous system is competent to respond to the hormone. previous experiments have shown that the action of eclos ...	1986	3020427
differential effects of transforming growth factor type beta on the growth and function of adrenocortical cells in vitro.	transforming growth factor type beta (tgf-beta) suppresses basal as well as corticotropin (acth)-stimulated steroid formation by bovine adrenocortical cells in culture. the effect is dose dependent and is not accompanied by any change in adrenocortical cell growth. the minimum effective dose of tgf-beta is 4 x 10(-13) m (10 pg/ml), and maximal inhibition is observed at a concentration of 4 x 10(-11) m (1 ng/ml). a 16- to 20-hr incubation with tgf-beta is required to decrease steroidogenesis, and ...	1986	3020557
differential effects of transforming growth factor type beta on the growth and function of adrenocortical cells in vitro.	transforming growth factor type beta (tgf-beta) suppresses basal as well as corticotropin (acth)-stimulated steroid formation by bovine adrenocortical cells in culture. the effect is dose dependent and is not accompanied by any change in adrenocortical cell growth. the minimum effective dose of tgf-beta is 4 x 10(-13) m (10 pg/ml), and maximal inhibition is observed at a concentration of 4 x 10(-11) m (1 ng/ml). a 16- to 20-hr incubation with tgf-beta is required to decrease steroidogenesis, and ...	1986	3020557
exposure of sheep to natural aerosols of foot-and-mouth disease virus.	sheep taken individually and allowed to inhale air being drawn along a duct from a cabinet containing pigs acutely infected with foot-and-mouth disease virus for 10 or 15 minute periods were infected by doses as low as 10 tcid50 of virus. the most consistent and reliable indicators of infection were viraemia and seroconversion. the mean times from exposure to onset of viraemia, pyrexia and the appearance of vesicular lesions were 2.5, 3.8 and 4.7 days, respectively. neither the time from exposur ...	1986	3020658
diminishing morbidity and mortality of bone marrow transplantation.	the reasons for the operational failure of bone marrow transplantation (bmt) are familiar and varied. they are the following: nonmarrow toxicity of the preparative regimen, marrow toxicity of the preparative regimen, acute graft-versus-host disease (gvhd), opportunistic infections, especially viral infections and recurrence of hematologic malignancy in the case of transplants for leukemia. this review examines operational failure of bmt and presents measures for its prevention.	1986	3020798
1,25-dihydroxyvitamin d3 inhibits the clonogenic growth of transformed cells via its receptor.	anchorage-independent growth in soft agar is a unique property of transformed cells which is known to be correlated with tumorigenicity. we report here that 1,25-dihydroxyvitamin d3 suppresses colony formation by a number of cultured cancer cell lines in soft agar in a dose dependent manner with an id50 of 5-7 x 10(-10) m. this effect is also achieved with analogues of 1,25-dihydroxyvitamin d3 in accordance with their binding affinity for the hormone's receptor. only cells with 1,25-dihydroxyvit ...	2011	3021122
dna sequence and product analysis of the virf locus responsible for congo red binding and cell invasion in shigella flexneri 2a.	the dna sequence of virf, a locus associated with virulence and the ability to bind congo red in shigella flexneri 2a that is located on a 140-megadalton (230-kilobase) plasmid, was determined and analyzed. it was rich in a and t. the direction of transcription of virf was determined by using a chloramphenicol resistance cartridge. an open reading frame readable in this direction was found. three proteins, 30, 27, and 21 kilodaltons, all corresponding to those predicted from the above sequence, ...	1986	3021627
characterization of shigella flexneri sequences encoding congo red binding (crb): conservation of multiple crb sequences and role of is1 in loss of the crb+ phenotype.	the ability to bind congo red (crb+) is associated with virulence of shigella flexneri and is encoded by a large, 220-kilobase plasmid. we cloned fragments of this plasmid to isolate the sequences encoding congo red binding, to determine the degree of conservation of these sequences among s. flexneri strains, and to study the molecular basis for loss of the crb+ phenotype. at least two separate bamhi fragments cloned into plasmid vectors encode congo red binding in e. coli or s. flexneri. one cr ...	1986	3021630