Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| differential effects of periopathogens on host protease inhibitors slpi, elafin, scca1, and scca2. | secretory leukocyte peptidase inhibitors (slpi), elafin, squamous cell carcinoma antigen 1 and 2 (scca1 and scca2) are specific endogenous serine protease inhibitors expressed by epithelial cells that prevent tissue damage from excessive proteolytic enzyme activity due to inflammation. to determine the effects of various periopathogens on these protease inhibitors, we utilized human gingival epithelial cells (gecs) challenged with cell-free bacteria supernatants of various periopathogens porphyr ... | 2010 | 21523231 |
| spiramycin resistance in human periodontitis microbiota. | purpose: the occurrence of in vitro resistance to therapeutic concentrations of spiramycin, amoxicillin, and metronidazole was determined for putative periodontal pathogens isolated in the united states. materials and methods: subgingival plaque specimens from 37 consecutive adults with untreated severe periodontitis were anaerobically cultured, and isolated putative periodontal pathogens were identified to a species level. in vitro resistance to spiramycin at 4 μg/ml, amoxicillin at 8 μg/ml, an ... | 2011 | 21524712 |
| fusobacterium nucleatum infection of colonic cells stimulates muc2 mucin and tumor necrosis factor-{alpha} | even though the etiology of inflammatory bowel disease is not completely known, it is influenced by the presence of normal gut microflora as well as-yet, unrecognized pathogens. the anaerobic, gram-negative bacterial species, fusobacterium nucleatum, is a common resident of the human mouth and gut and varies in its pathogenic potential. in this study, we demonstrate that highly invasive f. nucleatum isolates derived from the inflamed gut of crohn's disease patients evoked significantly greater m ... | 2011 | 21536792 |
| inhibitory effect of lactobacillus reuteri on periodontopathic and cariogenic bacteria. | the interaction between lactobacillus reuteri, a probiotic bacterium, and oral pathogenic bacteria have not been studied adequately. this study examined the effects of l. reuteri on the proliferation of periodontopathic bacteria including aggregatibacter actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, and tannerella forsythia, and on the formation of streptococcus mutans biofilms. human-derived l. reuteri strains (kctc 3594 and kctc 3678) and rat-derived l. reuteri kctc ... | 2011 | 21538238 |
| interaction of oral bacteria with gingival epithelial cell multilayers. | primary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. multilayers maintained at an air-liquid interface in low-calcium medium displayed differentiation and cytokeratin properties characteristic of junctional epithelium. multilayers were infected with fluorescently labeled porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, fusobacterium nucleatum or streptococcus gord ... | 2011 | 21545698 |
| antibacterial compounds from siraitia grosvenorii leaves. | luo han guo (lhg) fruits (siraitia grosvenorii swingle) have been used as traditional medicine in china for centuries to treat sore throats and coughs. however, lhg leaves are seldom used and minimal scientific information is available on them. in our recent study on the leaves of s. grosvenorii, the bioactive compounds β-amyrin (2), aloe emodin (5), aloe-emodin acetate (6), 5α,8α-epidioxy-24(r)-methylcholesta-6,22-dien-3β-ol (7) and p-hydroxyl benzyl acid (8), accompanied by n-hexadecaoic acid ... | 2011 | 21547839 |
| garlic allicin as a potential agent for controlling oral pathogens. | abstract garlic has been used medicinally throughout human history. allicin is considered the most therapeutic constituent of garlic. this study tested the antimicrobial activity of garlic allicin on oral pathogens associated with dental caries and periodontitis. allicin was found effective against all the tested bacteria. the broth dilution method revealed that planktonic growth of the cariogenic, gram-positive species streptococcus mutans, s. sobrinus, and actinomyces oris was inhibited by an ... | 2011 | 21548800 |
| oxidative stress response in the opportunistic oral pathogen fusobacterium nucleatum. | the anaerobic, gram-negative bacillus fusobacterium nucleatum plays a vital role in oral biofilm formation and the development of periodontal disease. the organism plays a central bridging role between early and late colonizers within dental plaque and plays a protective role against reactive oxygen species. using a two-dimensional gel electrophoresis and mass spectrometry approach, we have annotated 78 proteins within the proteome of f. nucleatum subsp. nucleatum and identified those proteins w ... | 2011 | 21563313 |
| antimicrobial effect of adjunctive use of chlorhexidine mouthrinse in untreated gingivitis: a randomized, placebo-controlled study. | becerik s, türkoğlu o, emingil g, vural c, özdemir g, atilla g. anti-microbial effect of adjunctive use of chlorhexidine mouthrinse in untreated gingivitis: a randomized, placebo-controlled study. apmis 2011; 119: 364-72. the aim of this study was to examine the effectiveness of chlorhexidine mouthrinse (chx) in addition to daily plaque control on subgingival microbiota in patients with untreated gingivitis. fifty gingivitis patients were randomized to chx or placebo groups. chx group rinsed wit ... | 2011 | 21569094 |
| characteristics and outcomes of fusobacterium nucleatum bacteremia-a 6-year experience at a tertiary care hospital in northern taiwan. | fusobacterium nucleatum bacteremia is critical and not well defined. to identify the clinical characteristics and outcomes, we conducted a retrospective review of hospitalized patients from january 2004 to december 2009 at a tertiary center in northern taiwan. fifty-seven patients were enrolled. the mean age was 58.1 years, and the mean pitt bacteremia score was 4.7. males predominated (59.6%), and the overall 30-day mortality rate was up to 47.4%. malignancy was the major comorbidity (26/57, 45 ... | 2011 | 21596220 |
| [weight loss and dyspnea in a 46-year-old woman.] | 2011 | 21601965 | |
| experimental alveolitis in rats: microbiological, acute phase response and histometric characterization of delayed alveolar healing. | the pathogenesis of alveolitis is not well known and therefore experimental situations that mimic some features of this disease should be developed. | 2011 | 21625744 |
| [prevalence of helicobacter pylori detected by real-time pcr in the subgingival plaque of patients with chronic periodontitis]. | helicobacter pylori plays a significant role in gastric disease. the aim of the present pilot study was to evaluate the prevalence of h. pylori detected by real-time polymerase chain reaction (real-time pcr) in the sub-gingival plaque of chronic periodontitis patients and to determine periodontopathogens profile of positive sites at h. pylori. | 2011 | 21688602 |
| a novel organotypic dento-epithelial culture model: effect of fusobacterium nucleatum biofilm on b-defensin-2, -3, and ll-37 expression. | background: in the present study, the expression and localization of three epithelial peptides, human +¦-defensin (hbd)-2, -3, and cathelicidin (ll-37), were studied in an organotypic dento-epithelial (od-e) model exposed to fusobacterium nucleatum biofilm. methods: biofilm of f. nucleatum atcc 25586 or ahn 9508 were produced by culturing each strain on semipermeable membranes. the od-e model was constructed by seeding keratinocytes on fibroblast-containing collagen gels and by placing dentin pi ... | 2011 | 21692631 |
| Tongue piercing: the effect of material on microbiological findings. | Biofilms on oral piercings may serve as a bacterial reservoir and lead to systemic bacteremia or local transmission of pathogenic microbiota. The use of piercing materials which are less susceptible to biofilm accumulation could contribute to prevention of problems. The present study investigated whether there are microbiological differences in bacterial samples collected from tongue piercings made of different materials. | 2011 | 21700161 |
| spontaneously draining acute otitis media in children: an observational study of clinical findings, microbiology and clinical course. | abstract objectives: to study the outcome of acute otitis media (aom) with otorrhoea in children managed initially without antibiotics, in relation to bacterial and clinical findings, and to identify those who may benefit from antibiotics. methods: otherwise healthy, not otitis prone children aged 2-16 y, presenting with aom with spontaneous otorrhoea, were recruited from primary care and followed at selected ear, nose and throat (ent) clinics. specimens for bacterial investigations were obtaine ... | 2011 | 21736512 |
| molecular sensing by the aptamer domain of the fmn riboswitch: a general model for ligand binding by conformational selection. | understanding the nature of the free state of riboswitch aptamers is important for illuminating common themes in gene regulation by riboswitches. prior evidence indicated the flavin mononucleotide (fmn)-binding riboswitch aptamer adopted a 'bound-like' structure in absence of fmn, suggesting only local conformational changes upon ligand binding. in the scope of pinpointing the general nature of such changes at the nucleotide level, we performed shape mapping experiments using the aptamer domain ... | 2011 | 21745821 |
| antimicrobial effects of o-cymen-5-ol and zinc, alone & in combination in simple solutions and toothpaste formulations. | this study aimed to evaluate antimicrobial effects of an o-cymen-5-ol/zinc system. | 2011 | 21762153 |
| analysis of the antimicrobial susceptibility of anaerobic bacteria isolated from endodontic infections in brazil during a period of nine years. | the purpose of this study was to analyze the susceptibility of some anaerobic species isolated from a brazilian population at different periods of time by determining a pattern of development of resistance to frequently prescribed antibiotics in endodontics. | 2011 | 21763894 |
| microbiological aspects of human mandibular subperiosteal dental implants. | abstract purpose: clinical, microbiological and biochemical features of human mandibular subperiosteal dental implants exhibiting peri-implantitis were compared to those experiencing long-term peri-implant health.materials and methods: after evaluation of clinical parameters, submucosal plaque biofilm samples were obtained from permucosal implant abutment posts exhibiting probing depths = 5 mm and bleeding on probing in subjects with peri-implantitis (n = 3), and from posts with peri-implant hea ... | 2011 | 21767207 |
| identification of an l-methionine {gamma}-lyase involved in the production of hydrogen sulfide from l-cysteine in fusobacterium nucleatum subsp. nucleatum atcc 25586. | fusobacterium nucleatum produces an abundance of hydrogen sulfide (h(2)s) in the oral cavity, which is mediated by several enzymes. the identification and characterization of three distinct enzymes (fn0625, fn1055 and fn1220) in f. nucleatum that catalyze the production of h(2)s from l-cysteine has been reported. in the current study, a novel enzyme involved in the production of h(2)s in f. nucleatum atcc 25586, whose molecular mass had been estimated to be approximately 130-kda, was identified ... | 2011 | 21798982 |
| Simultaneous detection of periodontal pathogens in subgingival plaque and placenta of women with hypertension in pregnancy. | BACKGROUND: There are many studies documenting increased prevalence of periodontal infection in women with preeclampsia. But, very few studies have attempted to establish causal relationship between the two. OBJECTIVE: To find out causal circumstantial evidence by isolating specific periodontal pathogens in oral and placental samples. MATERIALS AND METHODS: Antenatal periodontal screening and subgingival plaque collection was carried out in ten women with hypertension in pregnancy and ten normot ... | 2011 | 21830010 |
| invasive potential of gut mucosa-derived fusobacterium nucleatum positively correlates with ibd status of the host. | fusobacterium nucleatum is a heterogeneous oral pathogen that is also a common resident of the human gut mucosa. given that some strains of f. nucleatum are known to be invasive and proinflammatory in the oral mucosa, we compared strains isolated from patients with inflammatory bowel disease (ibd) with strains isolated from healthy controls to determine 1) whether this species was more commonly associated with ibd patients; and 2) whether gut-derived f. nucleatum strains from ibd patients showed ... | 2011 | 21830275 |
| efficacy of chlorhexidine digluconate-containing formulations and other mouthrinses against periodontopathogenic microorganisms. | objective: to determine in vitro the action of chlorhexidine digluconate and different commercially available mouthrinses on oral microorganisms. method and materials: minimal inhibitory concentrations and possible induction of resistance by chlorhexidine digluconate, an essential oil-containing mouthwash and an amine fluoride/stannous fluoride solution, were determined against microorganisms normally found in the oral cavity (10 streptococci, 2 enterobacteria, 1 candida albicans, 8 porphyromona ... | 2011 | 21842009 |
| analysis of symptomatic and asymptomatic primary root canal infections in adult norwegian patients. | this molecular study analyzed the microbiota of primary root canal infections from adult norwegian patients. | 2011 | 21846535 |
| Nonvolatile S-alk(en)ylthio-L-cysteine derivatives in fresh onion (Allium cepa L. cultivar). | The L-cysteine derivatives (R)-2-amino-3-(methyldisulfanyl)propanoic acid (S-methylthio-L-cysteine), (R)-2-amino-3-(propyldisulfanyl)propanoic acid (S-propylthio-L-cysteine), (R)-2-amino-3-(1-propenyldisulfanyl)propanoic acid (S-(1-propenylthio)-L-cysteine), and (R)-2-amino-3-(2-propenyldisulfanyl)propanoic acid (S-allylthio-L-cysteine) were prepared from 3-[(methoxycarbonyl)dithio]-L-alanine, obtained from the reaction of L-cysteine with methoxycarbonylsulfenyl chloride. The occurrence of these ... | 2011 | 21854077 |
| evaluation of the subgingival microbiota of alcoholic and non-alcoholic individuals. | the aim of this study was to evaluate the composition of the subgingival microbiota of alcoholic and non-alcoholic individuals. | 2011 | 21864643 |
| Comparative evaluation of two structurally related flavonoids, isoliquiritigenin and liquiritigenin, for their oral infection therapeutic potential. | Isoliquiritigenin (1) and liquiritigenin (2) are structurally related flavonoids found in a variety of plants. The purpose of this study was to perform a comparative analysis of biological properties of these compounds in regard to their therapeutic potential for oral infections. Compound 1 demonstrated significant antibacterial activity against three major periodontopathogens, Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. In contrast, 2 exerted less pronounced ef ... | 2011 | 21866899 |
| salivary β-galactosidase activity affects physiological oral malodour. | previous reports have associated salivary β-galactosidase activity with non-periodontopathic oral malodour. in this study, we investigated the localization of β-galactosidase and elucidated the relationship between its enzymatic activity and physiological oral malodour. | 2012 | 21885033 |
| Detection of odoriferous subgingival and tongue microbiota in diabetic and nondiabetic patients with oral malodor using polymerase chain reaction. | Halitosis has been correlated with the concentration of volatile sulfur compounds (VSCs) produced in the oral cavity by metabolic activity of bacteria colonizing the periodontal pockets and the dorsum of the tongue. It has been assumed that there is a relationship between periodontal disease and diabetes mellitus. | 2011 | 21891897 |
| Levels of periodontal pathogens in neonatal gastric aspirates and possible maternal sites of origin. | Maternal periodontal infection has been recognized as a risk factor for preterm and low birthweight infants. It is suspected that pathogens causing periodontal disease may translocate to the amniotic cavity and so contribute to triggering an adverse pregnancy outcome. This study aimed to determine levels and proportions of periodontal bacteria in neonatal gastric aspirates obtained from complicated pregnancies and the respective maternal oral and vaginal samples using a quantitative polymerase c ... | 2011 | 21896155 |
| antibacterial effects of chemomechanical instrumentation and calcium hydroxide in primary teeth with pulp necrosis. | this study's purposes were to: evaluate the antibacterial effect of chemomechanical instrumentation and a calcium hydroxide-based dressing in primary teeth with pulp necrosis secondary to trauma; and detect the presence of fusobacterium nucleatum and black-pigmented rods in the canals of these teeth. | 2011 | 21902996 |
| fusobacterium nucleatum and human beta-defensins modulate the release of antimicrobial chemokine ccl20/macrophage inflammatory protein 3α. | cells of the innate immune system regulate immune responses through the production of antimicrobial peptides, chemokines, and cytokines, including human beta-defensins (hbds) and ccl20. in this study, we examined the kinetics of primary human oral epithelial cell (hoec) production of ccl20 and hbds in response to fusobacterium nucleatum, a commensal bacterium of the oral cavity, which we previously showed promotes hoec induction of hbds. hoecs secrete maximal levels of ccl20 at 6 h, following st ... | 2011 | 21911466 |
| a role of oral bacteria in bisphosphonate-induced osteonecrosis of the jaw. | no consensus has yet been reached to associate oral bacteria conclusively with the etio-pathogenesis of bisphosphonate-induced osteonecrosis of the jaw (bonj). therefore, the present study examined the effects of oral bacteria on the development of bonj-like lesions in a mouse model. in the pamidronate (pam)-treated mice, but not control non-drug-treated mice, tooth extraction followed by oral infection with fusobacterium nucleatum caused bonj-like lesions and delayed epithelial healing, both of ... | 2011 | 21921248 |
| colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers. | gerodontology 2011; doi: 10.1111/j.1741-2358.2011.00506.x colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers objective: the purpose of this study was to investigate colonisation by periodontopathic bacteria and the sites of colonisation in elderly upper and lower complete denture wearers. we also investigated the relationship between level of oral hygiene and colonisation by periodontopathic bacteria. materials and methods: forty edentulous and 37 dentate ... | 2011 | 21929616 |
| synergy in biofilm formation between fusobacterium nucleatum and prevotella species. | the formation of biofilm by anaerobic, gram-negative bacteria in the subgingival crevice plays an important role in the development of chronic periodontitis. the aim of this study was to characterize the role of coaggregation between fusobacterium nucleatum and prevotella species in biofilm formation. coaggregation between f. nucleatum and prevotella species was determined by visual assay. effect of co-culture of the species on biofilm formation was assessed by crystal violet staining. effect of ... | 2011 | 21945879 |
| antibacterial activity of povidone-iodine against an artificial biofilm of porphyromonas gingivalis and fusobacterium nucleatum. | objective: to investigate the antibacterial activity of povidone-iodine (pvp-i) on an artificial dual species biofilm of periodontal pathogens. design: porphyromonas gingivalis or fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (pcm) tissue culture inserts. after incubation for 72h, pvp-i solutions were applied to the biofilm for the time period ranging from 0.5 to 5min. after addition of a deactivator, each pcm was removed and the biofilm on the pcm was s ... | 2011 | 21958970 |
| effect of a self-etching adhesive containing an antibacterial monomer on clinical periodontal parameters and subgingival microbiologic composition in orthodontic patients. | the aims of this study were to evaluate the effect of a self-etching adhesive system containing an antibacterial monomer on periodontal health and subgingival microbiologic composition in orthodontic patients and to compare it with a conventional adhesive system. | 2011 | 21967952 |
| isolation and molecular identification of fusobacterium nucleatum from nigerian patients with oro-facial infections. | fusobacterium nucleatum is one of the most common anaerobic bacteria present in the oral cavity and is often isolated from infections involving other body sites. | 2011 | 21984462 |
| The role of aggregation in Fusobacterium nucleatum- induced immune cell death. | Fusobacterium nucleatum, an anaerobic oral bacterium, has been shown to be highly abundant in endodontic infections. Its role in these infections remains unclear. Previous studies have shown that F. nucleatum could aggregate immune cells. We have demonstrated that F. nucleatum can induce significant apoptosis in peripheral blood mononuclear cells (PBMCs). In this in vitro study, we sought to determine what role this aggregation phenomenon has on the induction of apoptosis in PBMCs. | 2011 | 22000457 |
| fusobacterium nucleatum prosthetic hip infection in an adult with sickle cell-beta thalassemia. | fusobacterium nucleatum is an anaerobic gram-negative bacillus commensal to the human oropharynx and gastrointestinal tract which causes an array of human infection, yet it has never been associated with infection of prosthetic joints. we report the first case of prosthetic hip infection caused by f. nucleatum in a man with sickle cell-beta thalassemia. | 2011 | 22002730 |
| Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma. | An estimated 15% or more of the cancer burden worldwide is attributable to known infectious agents. We screened colorectal carcinoma and matched normal tissue specimens using RNA-seq followed by host sequence subtraction and found marked over-representation of Fusobacterium nucleatum sequences in tumors relative to control specimens. F. nucleatum is an invasive anaerobe that has been linked previously to periodontitis and appendicitis, but not to cancer. Fusobacteria are rare constituents of the ... | 2011 | 22009989 |
| limited in-depth invasion of fusobacterium nucleatum into in vitro reconstructed human gingiva. | objective: fusobacterium nucleatum is an opportunistic pathogen with a key role in subgingival plaque formation and it is found in increased numbers in periodontally affected sites. this study aimed to investigate the potential of f. nucleatum to penetrate and induce alterations in an in vitro reconstructed human gingival mucosa model. methods: three-dimensional (3d) organotypic models of human gingiva were engineered using primary gingival keratinocytes and fibroblasts. the reconstructed tissue ... | 2011 | 22024403 |
| The comparative evaluation of xanthan gel with chlorhexidine (Chlosite) in smokers and non-smokers: A clinical and microbiological assessment. | Prevention of the periodontal disease progression is the primary goal of periodontal therapy. When conventional therapy is found inadequate to attain periodontal health in chronic periodontitis, local antimicrobial agents have been used as an adjunct with scaling and root planning (SRP) which has reproduced encouraging results. Hence, this study was undertaken to evaluate the new sustained released local drug Chlosite clinically and microbiologically in smokers and non-smokers. | 2011 | 22028508 |
| fusobacterium nucleatum adhesin fada binds vascular endothelial cadherin and alters endothelial integrity. | fusobacterium nucleatum is a gram-negative oral anaerobe, capable of systemic dissemination causing infections and abscesses, often in mixed-species, at different body sites. we have shown previously that f. nucleatum adheres to and invades host epithelial and endothelial cells via a novel fada adhesin. in this study, vascular endothelial (ve)-cadherin, a member of the cadherin family and a cell-cell junction molecule, was identified as the endothelial receptor for fada, required for f. nucleatu ... | 2011 | 22040113 |
| fusobacterium nucleatum: an emerging gut pathogen? | the gram-negative, non-sporulating, obligately anaerobic species, fusobacterium nucleatum, is rapidly gaining notoriety as a pathogen with a surprising number of associated diseases. recently, we have found that f. nucleatum is a more common resident of the gi tract than originally thought, and thus, through several studies, we have attempted to determine its gut-relevant potential for virulence. we have found that f. nucleatum possesses a number of pathogenic traits with relevance to gut diseas ... | 2011 | 22067936 |
| Isoflavonoids and Coumarins from Glycyrrhiza uralensis: Antibacterial Activity against Oral Pathogens and Conversion of Isoflavans into Isoflavan-Quinones during Purification. | Phytochemical investigation of a supercritical fluid extract of Glycyrrhiza uralensis has led to the isolation of 20 known isoflavonoids and coumarins, and glycycarpan (7), a new pterocarpan. The presence of two isoflavan-quinones, licoriquinone A (8) and licoriquinone B (9), in a fraction subjected to gel filtration on Sephadex LH-20 is due to suspected metal-catalyzed oxidative degradation of licoricidin (1) and licorisoflavan A (2). The major compounds in the extract, as well as 8, were evalu ... | 2011 | 22074222 |
| antimicrobial efficacy of denture adhesives on some oral malodor-related microbes. | the objective of the study was to determine the antimicrobial efficacy of three denture adhesives toward streptococcus oralis, mutans, prevotella oralis and fusobacterium nucleatum. adhesives used were corega ultra(®), fixodent pro original(®) and biotene(®) denture grip. for streptococcus oralis and streptococcus mutans, four tubes of trypticase soy broth 10 ml and 1 g denture of adhesive were used. in addition four tubes of trypticase soy broth 10 ml without any denture adhesive was employed a ... | 2011 | 22075753 |
| A case of craniocervical abscess with sinus thrombosis in Lemierre?s syndrome. | Abstract A 74-year old gentleman with a cranio-cervical abscess failed to improve after incision and drainage and standard intravenous antibiotic therapy. Imaging demonstrated thrombosis of the internal jugular vein, sigmoid sinus and transverse sinus; and microbiological analysis isolated Fusobacterium nucleatum. The diagnosis of Lemierre's syndrome was confirmed, and he was effectively treated with appropriate antibiotics and anti-coagulation. | 2011 | 22077587 |
| investigation of six selected bacterial species in endo-periodontal lesions. | didilescu ac, rusu d, anghel a, nica l, iliescu a, greabu m, bancescu g, stratul si. investigation of six selected bacterial species in endo-periodontal lesions. international endodontic journal, 45, 282-293, 2012. abstract: aim to investigate and determine possible associations of six tested bacteria belonging to 'orange' and 'green' complexes, in endo-periodontal lesions: parvimonas micra, fusobacterium nucleatum, campylobacter rectus, eubacterium nodatum, eikenella corrodens and capnocytopha ... | 2011 | 22077868 |
| colorectal cancer: fusobacterium nucleatum found in colon cancer tissue--could an infection cause colorectal cancer? | 2011 | 22083120 | |
| [septic arthritis due to fusobacterium nucleatum in an immunocompetent patient.] | 2011 | 22089068 | |
| Signal peptide of FadA adhesin from Fusobacterium nucleatum plays a novel structural role by modulating the filament's length and width. | FadA, a novel adhesin of periodontal pathogen Fusobacterium nucleatum is composed of two forms, pre-FadA and mature FadA (mFadA), constituting the functional FadA complex (FadAc). By electron microscopy, we observed that mFadA formed uniformly long and thin filaments, while FadAc formed heterogeneous filaments of varying lengths and widths, as well as "knots". Mutants in signal peptide or in the non-alpha-helical loop retaining heterogeneous structures had binding activity while those forming ag ... | 2012 | 22108653 |
| extracellular deoxyribonuclease production by periodontal bacteria. | palmer lj, chapple ilc, wright hj, roberts a, cooper pr. extracellular deoxyribonuclease production by periodontal bacteria. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01451.x © 2011 john wiley & sons a/s background and objective: whilst certain bacteria have long been known to secrete extracellular deoxyribonuclease (dnase), the purpose in microbial physiology was unclear. recently, however, this enzyme has been demonstrated to confer enhanced virulence, enabling bacteria to evade the ... | 2011 | 22150619 |
| The adhesion of oral bacteria to modified titanium surfaces: role of plasma proteins and electrostatic forces. | OBJECTIVES: Modifications of titanium (Ti) implant surfaces have a significant effect on early biofilm formation and the outcome of implant procedures. The aim of this study was to examine the role of plasma proteins and electrostatic forces in the adhesion mechanism of oral bacteria to modified Ti surfaces. MATERIALS AND METHODS: Ti discs with three different types of surface modifications, machined, acid-etched, and acid-etched and blasted, were examined for adhesion of oral bacteria: Streptoc ... | 2011 | 22150723 |
| Antibacterial activity of sphingoid bases and fatty acids against gram-positive bacteria and gram-negative bacteria. | There is growing evidence that the role of lipids in innate immunity is more important than previously realized. How lipids interact with bacteria to achieve a level of protection, however, is still poorly understood. To begin to address mechanisms of antibacterial activity, we determined minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of lipids common to the skin and oral cavity: sphingoid bases D-sphingosine, phytosphingosine, and dihydrosphingosine, and f ... | 2011 | 22155833 |
| Oral Bacterial DNA Differ in Their Ability to Induce Inflammatory Responses in Human Monocytic Cell Lines. | BACKGROUND: DNA of periodontal pathogens, Porphyromonas gingivalis (Pg-DNA) and Tannerella forsythia, stimulate cytokine production in human monocytic cells (THP-1) through Toll like receptor 9 (TLR-9) and nuclear factor kappa B signaling. Fusobacterium nucleatum is one of the most frequently isolated bacteria in periodontally-diseased tissues and reported to synergize with Porhyromonas gingivalis enhancing the pathogenicity. We investigated inflammatory mediator production in THP-1 cells challe ... | 2011 | 22166166 |
| Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis. | OBJECTIVE: The aim of this study was to determine the profiles of periodontopathogenic bacteria in a Chinese population using quantitative real-time polymerase chain reaction (qRT-PCR). MATERIALS AND METHODS: Twenty-four periodontally healthy Chinese subjects and 60 patients with chronic periodontitis (CP) were enrolled in this cross-sectional study. qRT-PCR was used to quantify Aggregatibacter actinomycetemcomit ... | 2011 | 22169888 |
| Microbiological analysis of gingivitis in pediatric patients under orthodontic treatment. | The aim of this study was to determine the relationship between gingival inflammation and changes in bacteria of the gingival sulcus in children in orthodontic treatment with brackets. | 2011 | 22185242 |
| automated rna structure prediction uncovers a kink-turn linker in double glycine riboswitches. | the tertiary structures of functional rna molecules remain difficult to decipher. a new generation of automated rna structure prediction methods may help address these challenges but have not yet been experimentally validated. here we apply four prediction tools to a class of double glycine riboswitches that can bind two ligands cooperatively. a novel method (bppalign), rmdetect, jar3d, and rosetta 3d modeling give consistent predictions for a new stem p0 and a kink-turn motif. these elements st ... | 2011 | 22192063 |
| a five-species transcriptome array for oral mixed-biofilm studies. | oral polymicrobial interactions and biofilm formation are associated with initiation and progression of caries, gingivitis, and periodontitis. transcriptome studies of such interactions, allowing a first mechanistic insight, are hampered by current single-species array designs. | 2011 | 22194794 |
| adherence to streptococci facilitates fusobacterium nucleatum integration into an oral microbial community. | the development of multispecies oral microbial communities involves complex intra- and interspecies interactions at various levels. the ability to adhere to the resident bacteria or the biofilm matrix and overcome community resistance are among the key factors that determine whether a bacterium can integrate into a community. fusobacterium nucleatum is a prevalent gram-negative oral bacterial species that is able to adhere to a variety of oral microbes and has been implicated in playing an impor ... | 2011 | 22202886 |
| in vitro antibacterial activity of galls of quercus infectoria olivier against oral pathogens. | the galls of quercus infectoria are commonly used in malay traditional medicine to treat wound infections after childbirth. in india, they are employed traditionally as dental applications such as that in treatment of toothache and gingivitis. the aim of the present study was to evaluate the antibacterial activity of galls of quercus infectoria olivier against oral bacteria which are known to cause dental caries and periodontitis. methanol and acetone extracts were screened against two gram-posi ... | 2012 | 22203875 |
| adhesion of aggregatibacter actinomycetemcomitans and fusobacterium nucleatum on bioactive tio(2) surfaces. | bioactive tio(2) coatings have been found to enhance fibroblast adhesion and gingival attachment on the titanium surfaces, but no information is available whether the coatings also promote the adhesion of periodontal pathogens. | 2013 | 22220677 |
| investigation and quantification of key periodontal pathogens in patients with type 2 diabetes. | diabetes is a recognized risk factor for periodontitis. there are conflicting data regarding whether healthy diabetic patients or diabetic patients with chronic periodontitis have an altered subgingival microbiota compared with nondiabetic individuals. the aim of the present study was to detect quantitative differences in selected periodontopathogens in the subgingival plaque of diabetic patients using taqman quantitative pcr. | 2012 | 22220967 |
| subgingival microbiota in adult down syndrome periodontitis. | the subgingival microbiota in down syndrome and non-down syndrome adults receiving periodic dental care was examined for 40 bacterial species using checkerboard dna-dna hybridization and the results were related to clinical periodontal attachment loss. | 2012 | 22221039 |
| release of metronidazole from electrospun poly(l-lactide-co-d/l-lactide) fibers for local periodontitis treatment. | we aimed to achieve detailed biomaterials characterization of a drug delivery system for local periodontitis treatment based on electrospun metronidazole-loaded resorbable polylactide (pla) fibers. | 2012 | 22226009 |
| cytokine expression in response to root canal infection in gnotobiotic mice. | to examine cytokine expression profiles during periapical lesion development in response to synergetic human pathogens in a gnotobiotic mouse model. | 2012 | 22233143 |
| fusobacterium nucleatum biofilm induces epithelial migration in an organotypic model of dento-gingival junction. | background: effects of fusobacterium nucleatum biofilm on epithelial cell proliferation, apoptotic cell death and basement membrane constituent collagen iv production were examined in an organotypic dento-epithelial (od-e) model. methods: the od-e model was constructed by seeding keratinocytes on fibroblast-containing collagen gels and by placing tooth pieces on the top. a 3-day-old biofilm of either a laboratory strain (atcc 25586) or a clinical strain (ahn 9508) of f. nucleatum was placed on ... | 2012 | 22248219 |
| mucosal invasion by fusobacteria is a common feature of acute appendicitis in germany, russia, and china. | to investigate the geographic occurrence of mucosa-invading fusobacteria in acute appendicitis. | 2015 | 22249094 |
| synergistic effect on biofilm formation between fusobacterium nucleatum and capnocytophaga ochracea. | the formation of dental plaque biofilm by specific gram-negative rods and spirochetes plays an important role in the development of periodontal disease. the aim of this study was to characterize biofilm formation by fusobacterium nucleatum and capnocytophaga ochracea. coaggregation between f. nucleatum and capnocytophaga species was determined by visual assay. biofilm formation was assessed by crystal violet staining. enhancement of biofilm formation by f. nucleatum via soluble factor of c. ochr ... | 2012 | 22252100 |
| microbial profile of patients with periodontitis compared with healthy subjects. | objective: to define and compare the microbiologic profile of subgingival plaque in german patients with periodontitis (including aggressive and advanced chronic periodontitis) and healthy subjects and to determine significant association between isolates and clinical status. aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythia are major periodontal pathogens, though it is recognized that other species may also contribute to the pathogenesis of periodontal di ... | 2012 | 22257880 |
| necrotizing periodontal diseases in hiv-infected brazilian patients: a clinical and microbiologic descriptive study. | objective: nowadays, necrotizing periodontal diseases have a low prevalence; however, a better understanding of the etiopathogenesis of these diseases is necessary for determining more adequate preventive and therapeutic strategies. method and materials: from a pool of 1,232 hiv-infected patients, 15 presented with necrotizing periodontal diseases, which were evaluated by full-mouth periodontal clinical measurements. subgingival biofilm samples were collected from necrotizing lesions of six of t ... | 2012 | 22259811 |
| oral microbial biofilm stimulation of epithelial cell responses. | oral bacterial biofilms trigger chronic inflammatory responses in the host that can result in the tissue destructive events of periodontitis. however, the characteristics of the capacity of specific host cell types to respond to these biofilms remain ill-defined. this report describes the use of a novel model of bacterial biofilms to stimulate oral epithelial cells and profile select cytokines and chemokines that contribute to the local inflammatory environment in the periodontium. monoinfection ... | 2012 | 22266273 |
| detection of fusobacterium nucleatum in chorionic tissues of high-risk pregnant women. | this study was undertaken to investigate the existence of a periodontopathic bacterium, fusobacterium nucleatum, in chorionic tissues of pregnant women, and the effects of f. nucleatum on human chorion-derived cells. | 2012 | 22304677 |
| treponema denticola chymotrypsin-like proteinase (ctlp) integrates spirochaetes within oral microbial communities. | treponema denticola is found ubiquitously in the human oral cavity and is mainly associated with bacterial communities implicated in the establishment and development of periodontal disease. the ability to become integrated within biofilm communities is crucial to the growth and survival of oral bacteria, and involves inter-bacterial coaggregation, metabolic cooperation, and synergy against host defences. in this article we show that the chymotrypsin-like proteinase (ctlp), found within a high-m ... | 2012 | 22313692 |
| reactivation of latent hiv-1 by a wide variety of butyric acid-producing bacteria. | latently infected cells harbor human immunodeficiency virus type 1 (hiv-1) proviral dna copies integrated in heterochromatin, allowing persistence of transcriptionally silent proviruses. it is widely accepted that hypoacetylation of histone proteins by histone deacetylases (hdacs) is involved in maintaining the hiv-1 latency by repressing viral transcription. hiv-1 replication can be induced from latently infected cells by environmental factors, such as inflammation and co-infection with other m ... | 2012 | 22322557 |
| azithromycin as an adjunctive treatment of generalized severe chronic periodontitis: clinical, microbiologic, and biochemical parameters. | this study examines the efficacy of azithromycin in combination with non-surgical periodontal therapy on clinical and microbiologic parameters and gingival crevicular fluid (gcf) matrix metalloproteinases-8 (mmp-8) levels over 6 months in patients with severe generalized chronic periodontitis (cp). | 2012 | 22324488 |
| molecular analysis of 16s rrna genes identifies potentially periodontal pathogenic bacteria and archaea in the plaque of partially erupted third molars. | small subunit rrna sequencing and phylogenetic analysis were used to identify cultivable and uncultivable microorganisms present in the dental plaque of symptomatic and asymptomatic partially erupted third molars to determine the prevalence of putative periodontal pathogens in pericoronal sites. | 2012 | 22326171 |
| effect of periodontal pathogens on the metatranscriptome of a healthy multispecies biofilm model. | oral bacterial biofilms are highly complex microbial communities with up to 700 different bacterial taxa. we report here the use of metatranscriptomic analysis to study patterns of community gene expression in a multispecies biofilm model composed of species found in healthy oral biofilms (actinomyces naeslundii, lactobacillus casei, streptococcus mitis, veillonella parvula, and fusobacterium nucleatum) and the same biofilm plus the periodontopathogens porphyromonas gingivalis and aggregatibacte ... | 2012 | 22328675 |
| association between oral malodour and periodontal disease-related parameters in the general population. | to determine the association between halitosis detection and periodontal status in systemically healthy non-smokers and to assess whether halitosis was related to quantities of fusobacterium nucleatum and porphyromonas gingivalis on the tongue dorsum. | 2013 | 22339235 |
| [effect of periodontal surgery on the subgingival periodontal microbiota in artificial class iii furcation defects: an experimental study in monkeys]. | this study was to longitudinally evaluate the change of prevalence of five periodontal putative pathogens in the subgingival plaque of artificial class iii furcation defects at the three time-points, including before the establishment of furcation defects, before and 6 months after periodontal surgery. | 2012 | 22353894 |
| effects of oil drops containing lactobacillus salivarius wb21 on periodontal health and oral microbiota producing volatile sulfur compounds. | the objective of this paper is to evaluate the effects of oil drops containing lactobacillus salivarius wb21 on periodontal health and oral microbiota producing volatile sulfur compounds (vscs). for this study, 42 subjects were randomly assigned to receive oil samples containing l. salivarius wb21 or a placebo for two weeks. oral assessment and saliva collection were performed on days 1 and 15. bacterial analysis was performed using the real-time polymerase chain reaction and terminal restrictio ... | 2012 | 22368259 |
| internal decompressive craniectomy with craniotomy: a novel surgical therapy of giant frontal mucocele complicated by subdural empyema. | giant frontal mucocele (gfm) is an extremely rare cause of frontal lobe syndrome. subdural empyema (sde) is an uncommon complication of paranasal sinisutis, for which craniotomy and decompressive craniotomy are the most effective surgical procedures. | 2011 | 22368985 |
| anaerobic and microaerophilic pathogens in the biofilm formation on voice prostheses: a pilot study. | voice rehabilitation with voice prostheses is a standard therapy in laryngectomized patients. biofilm formation on the surface of the voice prostheses causes device failure and requires frequent replacements. studies analyzing the biofilm of voice prostheses have mainly focused on aerobic bacteria. anaerobic bacteria as an integral part of the biofilms on voice prostheses have not been investigated yet. | 2012 | 22374743 |
| fusobacterium nucleatum groel induces risk factors of atherosclerosis in human microvascular endothelial cells and apoe(-/-) mice. | infection and inflammation are risk factors in the initiation and progression of atherosclerosis. periodontitis is one of the most prevalent chronic inflammations of the oral cavity, and has been reported to be associated with systemic disease. in this study, we evaluated whether the heat-shock protein groel of fusobacterium nucleatum, one of the most prevalent bacteria in periodontitis, induces factors that predispose to atherosclerosis in human microvascular endothelial cells (hmec-1) and apol ... | 2011 | 22394469 |
| comparative evaluation of the effects of different photoablative laser irradiation protocols on the gingiva of periodontopathic patients. | we aimed at quantifying the presence of periodontopathogens in gingival biopsies from periodontitis patients treated with different photoablative lasers (diode gaas, er:yag, nd:yag, and co(2) lasers) and histologically analyzing their effects on the gingiva. | 2012 | 22401128 |
| characterization of microbiota of root canal-treated teeth with posttreatment disease. | this study evaluated the microbiota of root canals undergoing retreatment. the most prevalent taxa detected by checkerboard included propionibacterium species, fusobacterium nucleatum, streptococci, and pseudoramibacter alactolyticus. quantitative real-time pcr detected enterococcus faecalis and streptococci in 38% and 41% of the cases, comprising 9.76% and 65.78% of the total bacterial counts, respectively. the findings call into question the status of e. faecalis as the main pathogen and sugge ... | 2012 | 22403423 |
| in vitro evaluation of mtad and nisin in combination against common pathogens associated with root canal infection. | many pathogenic microorganisms were found in an infected root canal. the object of this study was to evaluate the effect of mtad in combination with nisin on the pathogens associated with root canal infection. | 2012 | 22414835 |
| canine periodontal disease control using a clindamycin hydrochloride gel. | stabilizing or reducing periodontal pocket depth can have a positive influence on the retention of teeth in dogs. a topical 2% clindamycin hydrochloride gel (chgel) was evaluated for the treatment of periodontal disease in dogs. the chgel formulation provides for the sustained erosion of the matrix, but also flows into the periodontal pocket as a viscous liquid, and then rapidly forms a gel that has mucoadhesive properties and also may function as a physical barrier to the introduction of bacter ... | 2011 | 22416621 |
| identification of oral bacterial dna in synovial fluid of patients with arthritis with native and failed prosthetic joints. | we examined the presence of bacterial dna in synovial fluids of native or clinically aseptically failed prosthetic joints from patients having periodontal disease and arthritis to determine whether there is bacterial spread from the oral cavity to the joints. | 2012 | 22426587 |
| giant frontal mucocele complicated by subdural empyema: treatment of a rare association. | giant frontal mucocele (gfm) is an extremely rare cause of frontal lobe syndrome. subdural empyema (sde) is an uncommon complication of paranasal sinisutis, for which craniotomy and decompressive craniotomy are the most effective surgical procedures. a 54-year-old man was brought unconscious to the emergency room where recurrent generalized seizures occurred. heroine abuse, hcv-related hepatitis, prolonged antibiotic therapy for treatment of purulent rhinorrhea, along with recent personality cha ... | 2012 | 22427297 |
| human neutrophil defensins and their effect on epithelial cells. | the aims of the present study include: 1) to localize human neutrophil defensin-1 (hnp-1) through hnp-3 in gingiva and in neutrophil extract-treated epithelial cell monolayers; 2) to determine the effects of hnp-1 on the epithelial cell viability, attachment, and spreading; and 3) to analyze the effect of hnp-1 on the bacterial adherence to epithelial cells. | 2013 | 22443519 |
| specificity of antimicrobial peptide ll-37 to neutralize periodontopathogenic lipopolysaccharide activity in human oral fibroblasts. | the antimicrobial peptide ll-37 is known to have a potent lipopolysaccharide (lps)-neutralizing activity in various cell types. because of observed heterogeneity within periodontopathogenic lps, the authors hypothesized that ll-37 had specificity to neutralize such lps activity. the present study, therefore, aims to investigate the lps-neutralizing activity of ll-37 to various periodontopathogenic lps in interleukin-8 (il-8) production after challenging them in human oral fibroblasts. | 2013 | 22443521 |
| adjunctive azithromycin in the treatment of aggressive periodontitis: microbiological findings of a 12-month randomized clinical trial. | to compare the subgingival microbiological outcomes of azithromycin or placebo as adjuncts to scaling and root planing (srp) in the treatment of aggressive periodontitis (agp), and to secondarily evaluate the microbiological effect of supragingival scaling in agp patients. | 2012 | 22445846 |
| direct recognition of fusobacterium nucleatum by the nk cell natural cytotoxicity receptor nkp46 aggravates periodontal disease. | periodontitis is a common human chronic inflammatory disease that results in the destruction of the tooth attachment apparatus and tooth loss. although infections with periopathogenic bacteria such as porphyromonas gingivalis (p. gingivalis) and fusobacterium nucleatum (f. nucleatum) are essential for inducing periodontitis, the nature and magnitude of the disease is determined by the host's immune response. here, we investigate the role played by the nk killer receptor nkp46 (ncr1 in mice), in ... | 2012 | 22457623 |
| mycoplasma pneumoniae preceding lemierre's syndrome due to fusobacterium nucleatum complicated by acute epstein-barr virus (ebv) infectious mononucleosis in an immunocompetent host. | we report an unusual case of lemierre's syndrome due to a rare species of fusobacterium, that is, fusobacterium nucleatum preceded by mycoplasma pneumoniae pharyngitis and followed later by epstein-barr virus infectious mononucleosis. | 2013 | 22464641 |
| butyric acid induces apoptosis in both human monocytes and lymphocytes equivalently. | short-chain fatty acids (scfas) are metabolites from anaerobic periodontopathic bacteria that induce apoptosis in immune cells such as lymphocytes, monocytes and macrophages. however, it remains unclear if scfas from pathogens induce apoptosis in monocytes/macrophages similarly with lymphocytes. this study investigated whether scfas-induced apoptosis is equal among the immunoregulatory cells. cell apoptosis of the employed human cells was evaluated after treatment with culture supernatants from ... | 2012 | 22466881 |
| fretibacterium fastidiosum gen. nov., sp. nov., isolated from the human oral cavity. | sgp1(t), a strain belonging to a lineage of the phylum synergistetes with no previously cultivated representatives was subjected to a comprehensive range of phenotypic and genotypic tests. for good growth the strain was dependent on co-culture with, or extracts from, selected other oral bacteria. cells of strain sgp1(t) were asaccharolytic and major amounts of acetic acid and moderate amounts of propionic acid were produced as end products of metabolism in peptone-yeast extract-glucose broth sup ... | 2013 | 22493171 |
| quantification of subgingival bacterial pathogens at different stages of periodontal diseases. | anaerobic gram-negative oral bacteria such as treponema denticola, aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsythia, campylobacter rectus, and fusobacterium nucleatum are closely associated with periodontal diseases. we measured the relative population (bacterial levels) of these oral pathogens in subgingival tissues of patients at different stages of korean chronic periodontal diseases. we divided the individuals into those with chronic gingivitis (g), moder ... | 2012 | 22526568 |