Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| efficient uptake of yersinia pseudotuberculosis via integrin receptors involves a rac1-arp 2/3 pathway that bypasses n-wasp function. | efficient uptake of yersinia pseudotuberculosis into cultured mammalian cells is the result of high-affinity binding of invasin to beta1 chain integrins. we demonstrate here that uptake requires rac1 and arp 2/3 function. bacterial uptake was stimulated by gtpgammas, but was inhibited in mammalian cells transfected with the interfering rac1-n17 derivative. rac1 was found to be activated in response to integrin engagement by invasin, whereas rac1 and arp 2/3 were found to be intensely localized a ... | 2001 | 11722735 |
| a role for n-wasp in invasin-promoted internalisation. | phagocytosis of yersinia pseudotuberculosis occurs through interaction of the bacterial protein invasin with beta1-integrins. here we report that n-wasp plays a role in internalisation of an invasin-expressing, avirulent strain of y. pseudotuberculosis. ectopic expression of n-wasp mutants, which affect recruitment of the arp2/3 complex to the phagosome, reduces uptake of yersinia. in addition, expression of the cdc42/rac-binding (crib) region of n-wasp has an inhibitory effect on uptake. using ... | 2001 | 11734206 |
| structure of the type iii secretion and substrate-binding domain of yersinia yoph phosphatase. | pathogenic strains of yersinia deploy a type iii secretion system to inject the potent tyrosine phosphatase yoph into host cells, where it dephosphorylates focal adhesion-associated substrates. the amino-terminal, non-catalytic domain of yoph is bifunctional; it is essential for the secretion and binding of the specific chaperone sych, but also targets the catalytic domain to substrates in the infected cell. we describe the 2.2 a resolution crystal structure of residues 1-129 of yoph from yersin ... | 2001 | 11737640 |
| the type iii secretion chaperone lcrh co-operates with yopd to establish a negative, regulatory loop for control of yop synthesis in yersinia pseudotuberculosis. | the enteropathogen yersinia pseudotuberculosis is a model system used to study the molecular mechanisms by which gram-negative pathogens secrete and subsequently translocate antihost effector proteins into target eukaryotic cells by a common type iii secretion system (ttss). in this process, yopd (yersinia outer protein d) is essential to establish regulatory control of yop synthesis and the ensuing translocation process. yopd function depends upon the non-secreted ttss chaperone lcrh (low-calci ... | 2001 | 11737648 |
| multifocal osteomyelitis and erythematous plaque associated with yersinia pseudotuberculosis infection. | 2001 | 11737757 | |
| nkt cells-containing inflammatory lesions induced by yersinia pseudotuberculosis glycolipids. | valpha14-expressing nkt (invnkt) cells are a population of non-conventional t lymphocytes (tl) that bridge mammalian innate and adaptive immunity. their role in infectious diseases and inflammatory processes is still largely ununderstood. a previous report has shown that an acute granulomatous-like reaction can be elicited by sub-cutaneous injection of mycobacterium tuberculosis glycolipids in mice, and that recruitment of invnkt cells at the injection site is instrumental in this process. here, ... | 2002 | 11750042 |
| interaction of yersinia enterocolitica and y. pseudotuberculosis with platelets. | yersinia enterocolitica is a bacterium capable of growth at 4 degrees c in donated blood and has been responsible for many deaths following transfusion. interaction of y. enterocolitica with blood cells is of interest in understanding the mechanisms of survival and growth in blood. the closely related organism y. pseudotuberculosis is known to invade platelets and cause platelet aggregation by a mechanism that involves expression of the chromosomal inv gene. yersinia isolates were made to expres ... | 2001 | 11761186 |
| dna adenine methylase overproduction in yersinia pseudotuberculosis alters yope expression and secretion and host immune responses to infection. | yersinia pseudotuberculosis mutants that overproduce the dna adenine methylase (dam) are highly attenuated, confer fully protective immune responses, and secrete several yersinia virulence proteins (yersinia outer proteins [yops]) under conditions that are nonpermissive for secretion in wild-type strains. we examined here the effects of dam overproduction on yersinia virulence determinant expression and secretion, as well as the host immune response to yersinia antigens. western blot analysis wi ... | 2002 | 11796641 |
| purification, crystallization and molecular symmetry of cdp-d-glucose 4,6-dehydratase from yersinia pseudotuberculosis. | the enzyme cdp-d-glucose 4,6-dehydratase (ec 4.2.1.45) is an nad(+)-dependent oxidoreductase which catalyzes the irreversible conversion of cdp-d-glucose to cdp-4-keto-6-deoxy-d-glucose. the product of this reaction is an intermediate in the synthesis of all cdp-linked 3,6-dideoxyhexoses, an important class of antigenic determinants found in the lipopolysaccharide layer of gram-negative bacteria. crystals of a recombinant form of this enzyme from yersinia pseudotuberculosis have been grown in tw ... | 2002 | 11807280 |
| occurrence of yersiniosis and listeriosis in wild boars in japan. | from december 1994 to february 1995, 131 wild boars (sus scrofa leucomysta) living in a mountainous area in japan were examined for yersiniosis and listeriosis. of 131 wild boars, 76 (58%) were males and 55 (42%) were females. four yersinia spp. including y. pseudotuberculosis, y. enterocolitica, y. frederiksenii, and y. aldovei, were isolated from 49 (37%) of 131 wild boars. yersinia pseudotuberculosis was isolated from five (4%) of 131 wild boars. all y. pseudotuberculosis isolates were seroty ... | 2002 | 11838217 |
| the invasion-associated type iii secretion system of salmonella enterica serovar typhimurium is necessary for intracellular proliferation and vacuole biogenesis in epithelial cells. | type iii secretion systems (ttss) are used by gram-negative pathogens to translocate proteins into eukaryotic host cells. salmonella enterica serovar typhimurium (s. typhimurium) has two of these specialized systems, which are encoded on separate salmonella pathogenicity islands (spi-1 and spi-2) and translocate unique sets of effectors. the specific roles of these systems in salmonella pathogenesis remain undefined, although spi-1 is required for bacterial invasion of epithelial cells and spi-2 ... | 2002 | 11856172 |
| yersinia pseudotuberculosis with limited genetic diversity is a common finding in tonsils of fattening pigs. | a total of 425 pig tonsils, including 210 tonsils from fattening pigs and 215 from sows, from seven different abattoirs in finland were studied for the occurrence of yersinia pseudotuberculosis from 1999 to 2000. the mean prevalence of y. pseudotuberculosis in fattening pig tonsils was 4%, varying from 0 to 10% between slaughterhouses. y. pseudotuberculosis was not recovered from sow tonsils. all 30 y. pseudotuberculosis isolates from eight pig tonsils were recovered after cold enrichment. seven ... | 2002 | 11899054 |
| [modern concepts on the relationship between the agents causing plague and pseudotuberculosis]. | the authors present published data and their own findings on the relationship between yersinia pestis and y. pseudotuberculosis and on the origination of y. pestis from y. pseudotuberculosis. study of microbiological and biochemical characteristics, external membrane protein spectra, and stability of chromosomal region of pigmentation brought the authors to a hypothesis that y. pestis minor subspecies (ssp. caucasica, altaica, hissarica, ulegeica) which are characterized by selective virulence o ... | 2002 | 11904922 |
| [liver abscesses due to yersinia pseudotuberculosis discloses a genetic hemochromatosis]. | 2001 | 11910996 | |
| induction of cell death in t lymphocytes by invasin via beta1-integrin. | ligand binding to beta1-integrins exerts multiple effects on cells of the immune system including adhesion, spreading, haptotaxis and costimulation of t cells activated by anti-cd3. here we show that a high-affinity ligand for beta1-integrins, the invasin (inv) protein of yersinia pseudotuberculosis, can induce cell death in t lymphocytes via a rapid process. partially purified native inv protein and an inv fusion protein caused apoptotic/necrotic caspase-independent cell death in t lymphocytes ... | 2002 | 11932920 |
| continuous exposure of mice to superantigenic toxins induces a high-level protracted expansion and an immunological memory in the toxin-reactive cd4+ t cells. | we analyzed the responses of several t cell fractions reactive with superantigenic toxins (sagts), staphylococcal enterotoxin a (sea), or yersinia pseudotuberculosis-derived mitogen (ypm) in mice implanted with mini-osmotic pumps filled with sea or ypm. in mice implanted with the sea pump, sea-reactive vbeta3(+)cd4(+) t cells exhibited a high-level protracted expansion for 30 days, and sea-reactive vbeta11(+)cd4(+) t cells exhibited a low-level protracted expansion. sea-reactive cd8(+) counterpa ... | 2002 | 11937534 |
| [interactions of certain sapronotic infection pathogens in mixed cultures on a solid medium at different temperatures]. | under experimental conditions within the time limit of 21-35 days the causative agents of sapronotic infections in binary cultures, grown on a solid medium at 37 degrees c, 25-27 degrees c and 6-8 degrees c, interacted with one another transbiotically and through contact, their interactions having the character of amensalism, commensalisms-amensalism, competitive equilibrium, antibiosis. irrespective of the initial density, a change in the species composition was observed, one of them playing th ... | 2002 | 11949246 |
| [ultrastructure of yersinia pseudotuberculosis in the process of their reversible transition into the dormant (non-culturable) state in association with blue-green algae]. | the ultrastructural organization of y. pseudotuberculosis in the process of the transition of vegetative cells into the dormant (noncultivable) state in interaction with blue-green algae of the species anabaena variabilis was studied by the method of transmission electron microscopy. the use of type specific y. pseudotuberculosis serum made it possible to identify y. pseudotuberculosis cells in the bacterial association and to find out whether their antigenic properties remained intact in time. ... | 2002 | 11949247 |
| yersinia pseudotuberculosis produces a cytotoxic necrotizing factor. | cell extracts from yersinia pseudotuberculosis induced multinucleation in hep-2 cells in a manner similar to the effect caused by escherichia coli cytotoxic necrotizing factor (cnf). the activity was not dependent on the yersinia 70-kb virulence plasmid, and the activity was not inhibited by antibodies capable of neutralizing e. coli cnf type 1. the nucleotide sequence of the yersinia cnf gene was 65.1% identical to the e. coli cnf gene. | 2002 | 11953417 |
| lcrq and sych function together at the ysc type iii secretion system in yersinia pestis to impose a hierarchy of secretion. | lcrq is a regulatory protein unique to yersinia. previous study in yersinia pseudotuberculosis and yersinia enterocolitica prompted the model in which lcrq negatively regulates the expression of a set of virulence proteins called yops, and its secretion upon activation of the yop secretion (ysc) type iii secretion system permits full induction of yops expression. in this study, we tested the hypothesis that lcrq's effects on yops expression might be indirect. excess lcrq was found to exert an in ... | 2002 | 11985718 |
| yersinia enterocolitica yopq: strain-dependent cytosolic accumulation and post-translational secretion. | yopq in yersinia enterocolitica (yopk in yersinia pseudotuberculosis) is a type iii secreted protein required for virulence of yersiniae. in this study yopq expression, secretion and nucleotide sequences of the corresponding yopq gene from different yersinia strains were analysed. the cytosolic accumulation differed significantly among serotypes of y. enterocolitica. these differences might be attributable to variations in the nucleotide sequence and their consequences on mrna secondary structur ... | 2002 | 11988520 |
| relationship of yersinia pseudotuberculosis o antigens ia, iia, and ivb: the iia gene cluster was derived from that of ivb. | o antigen is part of the lipopolysaccharide present in the outer membrane of gram-negative bacteria and is highly polymorphic. in this study, we obtained sequences of the o-antigen gene clusters for the yersinia pseudotuberculosis antigens ia, iia, and ivb. we propose that the iia gene cluster was derived from the ivb cluster, one of the very few cases in which a parent gene cluster is identified, and that the ia gene cluster could be a hybrid of the ivb and ib gene clusters. all three o antigen ... | 2002 | 12011023 |
| genome plasticity in yersinia pestis. | yersinia pestis, the causative agent of bubonic plague, emerged recently (<20000 years ago) as a clone of yersinia pseudotuberculosis. there is scant evidence of genome diversity in y. pestis, although it is possible to differentiate three biovars (antiqua, mediaevalis or orientalis) based on two biochemical tests. there are a few examples of restriction fragment length polymorphisms (rflps) within y. pestis; however, their genetic basis is poorly understood. in this study, six difference region ... | 2002 | 12055289 |
| role of the beta1-integrin cytoplasmic tail in mediating invasin-promoted internalization of yersinia. | invasin of yersinia pseudotuberculosis binds to beta1-integrins on host cells and triggers internalization of the bacterium. to elucidate the mechanism behind the beta1-integrin-mediated internalization of yersinia, a beta1-integrin-deficient cell line, gd25, transfected with wild-type beta1a, beta1b or different mutants of the beta1a subunit was used. both beta1a and beta1b bound to invasin-expressing bacteria, but only beta1a was able to mediate internalization of the bacteria. the cytoplasmic ... | 2002 | 12077358 |
| cas, fak and pyk2 function in diverse signaling cascades to promote yersinia uptake. | the interplay between pathogen-encoded virulence factors and host cell signaling networks is critical for both the establishment and clearance of microbial infections. yersinia uptake into host cells serves as an in vitro model for exploring how host cells respond to yersinia adherence. in this study, we provide insight into the molecular nature and regulation of signaling networks that contribute to the uptake process. using a reconstitution approach in fak(-/-) fibroblasts, we have been able t ... | 2002 | 12077360 |
| bacteria prove gutsy against inflammatory bowel disease. | 2002 | 12110235 | |
| the superantigen gene ypm is located in an unstable chromosomal locus of yersinia pseudotuberculosis. | yersinia pseudotuberculosis produces ypm (y. pseudotuberculosis-derived mitogen), a superantigenic toxin that exacerbates the virulence of the bacterium in vivo. to date, three alleles of the superantigen gene (ypma, ypmb, and ypmc) have been described. these genes are not found in all y. pseudotuberculosis strains and have a low gc content, suggesting their location on mobile genetic elements. to elucidate this question, the genetic environment of the superantigen-encoding genes was characteriz ... | 2002 | 12142419 |
| proteolytic cleavage of the flhb homologue yscu of yersinia pseudotuberculosis is essential for bacterial survival but not for type iii secretion. | pathogenic yersinia species employ a type iii secretion system (ttss) to target antihost factors, yop proteins, into eukaryotic cells. the secretion machinery is constituted of ca. 20 ysc proteins, nine of which show significant homology to components of the flagellar ttss. a key event in flagellar assembly is the switch from secreting-assembling hook substrates to filament substrates, a switch regulated by flhb and flik. the focus of this study is the flhb homologue yscu, a bacterial inner memb ... | 2002 | 12142420 |
| conformational analysis by cd and nmr spectroscopy of a peptide encompassing the amphipathic domain of yopd from yersinia. | to establish an infection, yersinia pseudotuberculosis utilizes a plasmid-encoded type iii secretion machine that permits the translocation of several anti-host factors into the cytosol of target eukaryotic cells. secreted yopd is essential for this process. pre-secretory stabilization of yopd is mediated by an interaction with its cognate chaperone, lcrh. yopd possesses lcrh binding domains located in the n-terminus and in a predicted amphipathic domain located near the c-terminus. this latter ... | 2002 | 12153562 |
| yersinia type iii secretion: send in the effectors. | pathogenic yersinia spp (yersinia pestis, yersinia pseudotuberculosis, and yersinia enterocolitica) have evolved an exquisite method for delivering powerful effectors into cells of the host immune system where they inhibit signaling cascades and block the cells' response to infection. understanding the molecular mechanisms of this system has provided insight into the processes of phagocytosis and inflammation. | 2002 | 12163464 |
| characterization of the ysa pathogenicity locus in the chromosome of yersinia enterocolitica and phylogeny analysis of type iii secretion systems. | several gram negative bacteria use a complex system called "type iii secretion system" (ttss) to engage their host. the archetype of ttss is the plasmid-encoded "yop virulon" shared by the three species of pathogenic yersinia (y. pestis, y. pseudotuberculosis, and y. enterocolitica). a second ttss, called ysa (for yersinia secretion apparatus) was recently described in y. enterocolitica 8081, a strain from serotype o:8. in this study, we describe the ysa locus from a127/90, another strain of ser ... | 2002 | 12165841 |
| the yersinia pseudotuberculosis yut protein, a new type of urea transporter homologous to eukaryotic channels and functionally interchangeable in vitro with the helicobacter pylori urei protein. | urea uptake in eukaryotes and prokaryotes occurs via diffusion or active transport across the cell membrane. facilitated diffusion of urea in both types of organisms requires a single-component channel. in bacteria, these transport systems allow rapid access of urease to its substrate, resulting in ammonia production, which is needed either for resistance to acidity or as a nitrogen source. in yersinia pseudotuberculosis, a ureolytic enteropathogenic bacterium, a gene of unknown function (yut) l ... | 2002 | 12180933 |
| the yada protein of yersinia pseudotuberculosis mediates high-efficiency uptake into human cells under environmental conditions in which invasin is repressed. | the yada protein is a major adhesin of yersinia pseudotuberculosis that promotes tight adhesion to mammalian cells by binding to extracellular matrix proteins. in this study, we first addressed the possibility of competitive interference of yada and the major invasive factor invasin and found that expression of yada in the presence of invasin affected neither the export nor the function of invasin in the outer membrane. furthermore, expression of yada promoted both bacterial adhesion and high-ef ... | 2002 | 12183532 |
| yersinia pseudotuberculosis infection in breeding monkeys: detection and analysis of strain diversity by pcr. | in the last three decades, several monkeys reared in outdoor/indoor-outdoor breeding colonies and cages of the primate research institute, kyoto university, died of yersiniosis caused by yersinia pseudotuberculosis, necessitating introduction of a method to detect the bacteria rapidly and thus allow preventive measures to be undertaken. a rapid nested polymerase chain reaction (pcr) method for identification of y. pseudotuberculosis in fecal samples and a random amplified polymorphic dna (rapd)- ... | 2002 | 12190853 |
| n-acylhomoserine lactones undergo lactonolysis in a ph-, temperature-, and acyl chain length-dependent manner during growth of yersinia pseudotuberculosis and pseudomonas aeruginosa. | in gram-negative bacterial pathogens, such as pseudomonas aeruginosa and yersinia pseudotuberculosis, cell-to-cell communication via the n-acylhomoserine lactone (ahl) signal molecules is involved in the cell population density-dependent control of genes associated with virulence. this phenomenon, termed quorum sensing, relies upon the accumulation of ahls to a threshold concentration at which target structural genes are activated. by using biosensors capable of detecting a range of ahls we obse ... | 2002 | 12228292 |
| solution structure and phosphopeptide binding to the n-terminal domain of yersinia yoph: comparison with a crystal structure. | virulence of pathogenic bacteria of the genus yersinia requires the injection of six effector proteins into the cytoplasm of host cells. the amino-terminal domain of one of these effectors, the tyrosine phosphatase yoph, is essential for translocation of yoph, as well as for targeting it to phosphotyrosine-containing substrates of the type pyxxp. we report the high-resolution solution structure of the n-terminal domain (residues 1-129) from the yersinia pseudotuberculosis yoph (yoph-nt) in compl ... | 2002 | 12234185 |
| genes encoding specific nickel transport systems flank the chromosomal urease locus of pathogenic yersiniae. | the transition metal nickel is an essential cofactor for a number of bacterial enzymes, one of which is urease. prior to its incorporation into metalloenzyme active sites, nickel must be imported into the cell. here, we report identification of two loci corresponding to nickel-specific transport systems in the gram-negative, ureolytic bacterium yersinia pseudotuberculosis. the loci are located on each side of the chromosomal urease gene cluster ureabcefgd and have the same orientation as the lat ... | 2002 | 12270829 |
| yersinia pseudotuberculosis harbors a type iv pilus gene cluster that contributes to pathogenicity. | fimbriae have been shown to play an essential role in the adhesion of pathogenic gram-negative bacteria to host cells. in the enteroinvasive bacterium yersinia pseudotuberculosis, we characterized a previously unknown 11-kb chromosomal locus involved in the synthesis of type iv pili. the locus consists of 11 open reading frames forming a polycistronic unit and encoding putative pil proteins, pillmnopqrsuvw. when introduced into escherichia coli, the y. pseudotuberculosis operon reconstituted bun ... | 2002 | 12379698 |
| isolation and characterization of the immunoglobulin-binding protein from yersinia pseudotuberculosis. | a high molecular weight immunoglobulin-binding protein localized on the surface of bacterial cells has been isolated from the protein fraction of the outer membrane of yersinia pseudotuberculosis, and its properties are described. the immunoglobulin-binding protein is a trypsin-resistant and temperature-sensitive beta-structured protein. as shown by maldi-tof mass spectrometry, after heating at 100 degrees c the molecular weight of the protein constituted 37.5 kd. the native protein is capable o ... | 2002 | 12387724 |
| yersinia v-antigen exploits toll-like receptor 2 and cd14 for interleukin 10-mediated immunosuppression. | a characteristic of the three human-pathogenic yersinia spp. (the plague agent yersinia pestis and the enteropathogenic yersinia pseudotuberculosis and yersinia enterocolitica) is the expression of the virulence (v)-antigen (lcrv). lcrv is a released protein which is involved in contact-induced secretion of yersinia antihost proteins and in evasion of the host's innate immune response. here we report that recombinant lcrv signals in a cd14- and toll-like receptor 2 (tlr2)-dependent fashion leadi ... | 2002 | 12391013 |
| crystal structure of a major secreted protein of mycobacterium tuberculosis-mpt63 at 1.5-a resolution. | mpt63 is a small, major secreted protein of unknown function from mycobacterium tuberculosis that has been shown to have immunogenic properties and has been implicated in virulence. a blast search identified that mpt63 has homologs only in other mycobacteria, and is therefore mycobacteria specific. as mpt63 is a secreted protein, mycobacteria specific, and implicated in virulence, mpt63 is an attractive drug target against the deadliest infectious disease, tuberculosis (tb). as part of the tb st ... | 2002 | 12441386 |
| [impact of yersinia pseudotuberculosis on the in vitro production of cytokines by whole blood cells of donors]. | the impact of two plasmid (47, 82 md), single plasmid (47 md) and non plasmid y. pseudotuberculosis strains, y. enterocolitica (47 md) as well as y. pseudotuberculosis superantigen (ypm) on the production of interleukin-1 (il-1), interleukin-6 (il-6), interferon-alpha (ifn = alpha) and tumor necrosis factor alpha (tnf-alpha) by whole blood cells obtained from donors was studied. all y. pseudotuberculosis and y. enterocolitica strains stimulated the production of ifn-alpha, il-1, il-6 and tnf-alp ... | 2002 | 12449699 |
| yersinia pseudotuberculosis bacteraemia in a kidney transplant patient. | 2002 | 12454242 | |
| gap activity of yersinia yope. | 2002 | 12474400 | |
| microtubule-dependent regulation of rho gtpases during internalisation of yersinia pseudotuberculosis. | internalisation of the human pathogen yersinia pseudotuberculosis via interaction of bacterial invasin with host beta1 integrins depends on the actin cytoskeleton and involves src family kinases, focal adhesion kinase, p130crk-associated substrate, proline-rich tyrosine kinase 2, rac, arp 2/3 complex and wasp family members. we show here that rho gtpases are regulated by the microtubule system during bacterial uptake. interfering with microtubule organisation using nocodazole or paclitaxel suppr ... | 2003 | 12505155 |
| [research on superantigenic toxins integrating bacteriology, immunology, bacterial toxins, and clinical infections diseases]. | 2002 | 12518385 | |
| mastitis caused by yersinia pseudotuberculosis in a cow. | 2002 | 12521250 | |
| [antibiotic properties of lactobacillus acidophilus, "narine" strain, and the ways for their improvement]. | l. acidophilus strain 317/402 "nariné" shows high antimicrobial activity with respect to pathogenic microorganisms. the degree of the activity of lactobacilli depends on cultivation conditions and the composition of the culture medium and its acidity, age of the culture, conditions of technological process, etc. the antimicrobial inhibition concentration of the product "nariné", ensuring in vitro activity against yersinia enterocolitica, y. pseudotuberculosis, vibrio nag, etc., has been establis ... | 2002 | 12525004 |
| [mode of sample preparation for pseudotuberculosis laboratory diagnosis by polymer chain reaction method]. | a mode of feces sample preparation was developed for polymerase chain reaction (pcr) assay. it was based on alkaline treatment of the material. this treatment killed the most part of indigenous microflora, whereas yersinia survived, because it was relatively resistant to alkaline. the mode was tested using human feces artificially contaminated with yersinia pseudotuberculosis. positive responses in samples containing 10(3)-10(8) microbial cells per ml were obtained by pcr assay with yersi and ye ... | 2002 | 12534270 |
| biochemical characterization of the yersinia yopt protease: cleavage site and recognition elements in rho gtpases. | the gram-negative bacterial pathogen yersinia delivers six effector proteins into the host cells to thwart the host innate immune response. one of the effectors, yopt, causes the disruption of the actin cytoskeleton and contributes to the inhibition of phagocytosis of the pathogen. yopt functions as a cysteine protease to cleave rho family gtpases. we have analyzed the yopt cleavage products of rho gtpases by tlc and determined their chemical structure by ms. amino acid labeling experiments were ... | 2003 | 12538863 |
| interaction between the yersinia protein tyrosine phosphatase yoph and eukaryotic cas/fyb is an important virulence mechanism. | the tyrosine phosphatase yoph is an essential virulence factor produced by pathogenic yersinia species. yoph is translocated into host cells via a type iii secretion system and its dephosphorylating activity causes disruption of focal complex structures and blockage of the phagocytic process. among the host cell targets of yoph are the focal adhesion proteins crk-associated substrate (p130cas) and focal adhesion kinase (fak) in epithelial cells, and p130cas and fyn-binding protein (fyb) in macro ... | 2003 | 12542470 |
| yersinia pestis and plague. | yersinia pestis is the aetiological agent of plague, a disease of humans that has potentially devastating consequences. evidence indicates that y. pestis evolved from yersinia pseudotuberculosis, an enteric pathogen that normally causes a relatively mild disease. although y. pestis is considered to be an obligate pathogen, the lifestyle of this organism is surprisingly complex. the bacteria are normally transmitted to humans from a flea vector, and y. pestis has a number of mechanisms which allo ... | 2003 | 12546664 |
| [chronic and lingering yersinia ileitis]. | to study the incidence, clinical manifestations, and diagnosis of chronic and lingering yersinia ileitis. | 2002 | 12577849 |
| acute renal failure in kawasaki disease. | 2003 | 12579412 | |
| role of toll-like receptor signaling in the apoptotic response of macrophages to yersinia infection. | macrophages encode several toll-like receptors (tlrs) that recognize bacterial components, such as lipoproteins (tlr2) or lipopolysaccharides (tlr4), and activate multiple signaling pathways. activation of transcription factor nf-kappab by tlr2 or tlr4 signaling promotes proinflammatory and cell survival responses. alternatively, tlr2 or tlr4 signaling can promote apoptosis if the activation of nf-kappab is blocked. the gram-negative bacterial pathogen yersinia pseudotuberculosis secretes into m ... | 2003 | 12595470 |
| proinflammatory signalling stimulated by the type iii translocation factor yopb is counteracted by multiple effectors in epithelial cells infected with yersinia pseudotuberculosis. | type iii secretion systems are used by several pathogens to translocate effector proteins into host cells. yersinia pseudotuberculosis delivers several yop effectors (e.g. yoph, yope and yopj) to counteract signalling responses during infection. yopb, yopd and lcrv are components of the translocation machinery. here, we demonstrate that a type iii translocation protein stimulates proinflammatory signalling in host cells, and that multiple effector yops counteract this response. to examine proinf ... | 2003 | 12603736 |
| tyea of yersinia pseudotuberculosis is involved in regulation of yop expression and is required for polarized translocation of yop effectors. | type iii secretion-dependent translocation of yop (yersinia outer proteins) effector proteins into host cells is an essential virulence mechanism common to the pathogenic yersinia species. one unique feature of this mechanism is the polarized secretion of yops, i.e. yops are only secreted at the site of contact with the host cell and not to the surrounding medium. in vitro, secretion occurs in ca2+-depleted media, a condition believed to somehow mimic cell contact. three proteins, yopn, lcrg and ... | 2003 | 12614462 |
| pcr detection of virulence genes in yersinia enterocolitica and yersinia pseudotuberculosis and investigation of virulence gene distribution. | pcr-based assays were developed for the detection of plasmid- and chromosome-borne virulence genes in yersinia enterocolitica and yersinia pseudotuberculosis, to investigate the distribution of these genes in isolates from various sources. the results of pcr genotyping, based on 5 virulence-associated genes of 140 strains of y. enterocolitica, were compared to phenotypic tests, such as biotyping and serotyping, and to virulence plasmid-associated properties such as calcium-dependent growth at 37 ... | 2003 | 12620874 |
| the yersinia virulence factor yopm forms a novel protein complex with two cellular kinases. | pathogenic yersinia contain a virulence plasmid that encodes genes for intracellular effectors, which neutralize the host immune response. one effector, yopm, is necessary for yersinia virulence, but its function in host cells is unknown. to identify potential cellular pathways affected by yopm, proteins that co-immunoprecipitate with yopm in mammalian cells were isolated and identified by mass spectrometry. results demonstrate that two kinases, protein kinase c-like 2 (prk2) and ribosomal s6 pr ... | 2003 | 12626518 |
| yscp and yscu regulate substrate specificity of the yersinia type iii secretion system. | pathogenic yersinia species use a type iii secretion system to inhibit phagocytosis by eukaryotic cells. at 37 degrees c, the secretion system is assembled, forming a needle-like structure on the bacterial cell surface. upon eukaryotic cell contact, six effector proteins, called yops, are translocated into the eukaryotic cell cytosol. here, we show that a yscp mutant exports an increased amount of the needle component yscf to the bacterial cell surface but is unable to efficiently secrete effect ... | 2003 | 12644497 |
| pathogenic yersinia enterocolitica strains increase the outer membrane permeability in response to environmental stimuli by modulating lipopolysaccharide fluidity and lipid a structure. | pathogenic biotypes of yersinia enterocolitica (serotypes o:3, o:8, o:9, and o:13), but not environmental biotypes (serotypes o:5, o:6, o:7,8, and o:7,8,13,19), increased their permeability to hydrophobic probes when they were grown at ph 5.5 or in egta-supplemented (ca(2+)-restricted) media at 37 degrees c. a similar observation was also made when representative strains of serotypes o:8 and o:5 were tested after brief contact with human monocytes. the increase in permeability was independent of ... | 2003 | 12654821 |
| disarming the invader. | type iii secretion systems are used by many gram-negative bacterial pathogens of animals and plants to deliver essential virulence factors into targeted host cells. the identification of chemical compounds that block the function of these systems is the first step toward developing chemical attenuation as an effective method for the treatment of infectious disease. | 2003 | 12670533 |
| targeting bacterial virulence: inhibitors of type iii secretion in yersinia. | agents that target bacterial virulence without detrimental effect on bacterial growth are useful chemical probes for studies of virulence and potential candidates for drug development. several gram-negative pathogens employ type iii secretion to evade the innate immune response of the host. screening of a chemical library with a luciferase reporter gene assay in viable yersinia pseudotuberculosis furnished several compounds that inhibit the reporter gene signal expressed from the yope promoter a ... | 2003 | 12670538 |
| expression of heterologous o-antigen in yersinia pestis kim does not affect virulence by the intravenous route. | all strains of yersinia pestis examined have been found to lack an o-antigen. in other members of the enterobacteriaceae, the rough phenotype often results in attenuation. however, y. pestis is the aetiological agent of bubonic plague. in evolving from the ancestral enteropathogenic yersinia pseudotuberculosis, and with the development of an arthropod-vectored systemic pathogenesis, smooth lps production is not necessary for y. pestis virulence and the metabolic burden has been alleviated by ina ... | 2003 | 12676866 |
| chlamydia trachomatis type iii secretion: evidence for a functional apparatus during early-cycle development. | the obligate intracellular bacterium chlamydia trachomatis occupies a parasitophorous vacuole termed an inclusion. during its intracellular developmental cycle, c. trachomatis maintains this intracellular niche, presumably by expressing a type iii secretion system, which deploys a set of host cell-interactive proteins including inclusion membrane-localized proteins termed incs. some incs are expressed and secreted by 2 h (early cycle) after infection, whereas the expression of type iii-specific ... | 2003 | 12694613 |
| dna modification and functional delivery into human cells using escherichia coli dh10b. | the availability of almost the complete human genome as cloned bac libraries represents a valuable resource for functional genomic analysis, which, however, has been somewhat limited by the ability to modify and transfer this dna into mammalian cells intact. here we report a novel comprehensive escherichia coli-based vector system for the modification, propagation and delivery of large human genomic bac clones into mammalian cells. the get recombination inducible homologous recombination system ... | 2003 | 12711696 |
| investigation of infectious agents associated with arthritis by reverse transcription pcr of bacterial rrna. | in reactive and postinfectious arthritis the joints are generally sterile but the presence of bacterial antigens and nucleic acids has been reported. to investigate whether organisms traffic to affected joints in these conditions, we performed reverse transcription pcr using universal primers to amplify any bacterial 16s rrna sequences present in synovial fluid. bacterial sequences were detected in most cases, even after treatment of the synovial fluid with dnase, implying the presence of bacter ... | 2003 | 12716447 |
| heat-stable serogroup-specific proteins of yersinia pseudotuberculosis. | a library of mabs to the species- and serogroup-specific epitopes of yersinia pseudotuberculosis serogroups i-vi was developed. these mabs recognized linear sequential protein epitopes, as shown by elisa and immunoblotting. using the mabs, y. pseudotuberculosis was found to produce serogroup-specific proteins, whose synthesis was dependent on cultivation temperature. these proteins appeared to be parts of heat-stable o-antigens prepared by heating y. pseudotuberculosis serogroups i-vi at 100 deg ... | 2003 | 12721314 |
| [yersiniosis (except the plague)]. | 2003 | 12722262 | |
| the biosynthesis of gdp-l-colitose: c-3 deoxygenation is catalyzed by a unique coenzyme b6-dependent enzyme. | l-colitose (1) is a 3,6-dideoxyhexose found in the o-antigen of gram-negative lipopoly-saccharides. while the biosynthesis of many deoxysugars have previously been investigated, l-colitose is distinct in that it originates from gdp-d-mannose. in contrast, other 3,6-dideoxyhexoses arise from cdp-d-glucose. therefore, the enzymes involved in the l-colitose biosynthetic pathway must be specifically tailored to utilize such a modified substrate. the mode for deoxygenation at c-3 of colitose is of pa ... | 2003 | 12733868 |
| isolation and expression of recombinant antibody fragments to the biological warfare pathogen brucella melitensis. | brucella melitensis is a highly infectious animal pathogen able to cause a recurring debilitating disease in humans and is therefore high on the list of biological warfare agents. immunoglobulin genes from mice immunized with gamma-irradiated b. melitensis strain 16m were used to construct a library that was screened by phage display against similarly prepared bacteria. the selected phage particles afforded a strong enzyme-linked immunosorbent assay (elisa) signal against gamma-irradiated b. mel ... | 2003 | 12738372 |
| interaction of yersinia enterocolitica with epithelial cells: invasin beyond invasion. | the chromosomally encoded inv gene product is an outer membrane protein that is functionally expressed in the enteropathogenic yersinia species yersinia enterocolitica and yersinia pseudotuberculosis. invasin protein is a high-affinity ligand for beta1 integrins and especially important in the early phase of intestinal infection for efficient translocation through the m cells located in the follicle-associated epithelium overlying the peyer's patches. in addition to bacterial internalization, ye ... | 2003 | 12755365 |
| a technique of intradermal injection of yersinia to study y. pestis physiopathology. | 2003 | 12756731 | |
| yersinia pseudotuberculosis harbors a type iv pilus gene cluster that contributes to pathogenicity. | 2003 | 12756734 | |
| characterisation of the type iii secretion protein yscu in yersinia pseudotuberculosis. yscu cleavage--dispensable for ttss but essential for survival. | 2003 | 12756738 | |
| characterization of infections with wild and mutant yersinia pseudotuberculosis strains in rabbit oral model. | 2003 | 12756740 | |
| impact of the yersinia pseudotuberculosis-derived mitogen (ypm) on the murine immune system. | 2003 | 12756744 | |
| role of t cells and gamma interferon in yersinia pseudotuberculosis-derived mitogen (ypm)-induced toxicity in mice. | 2003 | 12756745 | |
| defensive function of phagocytes in pseudotuberculosis. | 2003 | 12756750 | |
| yersinia pseudotuberculosis, its toxins and plant cells. | 2003 | 12756752 | |
| influence of yersinia pseudotuberculosis on the immunity of echinoderms. | 2003 | 12756753 | |
| acute and chronic experimental infection processes caused by yersinia pseudotuberculosis and evaluation of interleukin action on their development and outcome. | 2003 | 12756754 | |
| o antigen gene clusters of yersinia pseudotuberculosis. | 2003 | 12756757 | |
| function and regulation of the salmonella-like pmrf antimicrobial peptide resistance operon in yersinia pseudotuberculosis. | 2003 | 12756767 | |
| porin from yersinia pseudotuberculosis: cloning and analysis of primary structure. | 2003 | 12756768 | |
| function and regulation of the transcriptional activator rova of yersinia pseudotuberculosis. | 2003 | 12756772 | |
| molecular epidemiology of the five recent outbreaks of yersinia pseudotuberculosis in finland. | 2003 | 12756776 | |
| molecular virulence characteristics and kinetics of infection of yersinia pseudotuberculosis isolated from sick and healthy animals. | 2003 | 12756779 | |
| genetic (sero) typing of yersinia pseudotuberculosis. | 2003 | 12756783 | |
| molecular epidemiology of yersinia pseudotuberculosis. | 2003 | 12756788 | |
| yersinia pseudotuberculosis in pigs and pig houses in finland. | 2003 | 12756792 | |
| occurrence of yersinia pseudotuberculosis in iceberg lettuce and environment. | 2003 | 12756795 | |
| a rapid method for the detection of enteropathogenic yersinia in routine diagnostics of yersiniosis and pseudotuberculosis. | 2003 | 12756796 | |
| molecular epidemiological characterization of yersinia pseudotuberculosis circulating in different geographic areas of the russian federation. | 2003 | 12756797 | |
| pathogenic role of a superantigen in yersinia pseudotuberculosis infection. | 2003 | 12756809 | |
| attenuated yersinia pseudotuberculosis carrier vaccine for simultaneous antigen-specific cd4 and cd8 t-cell induction. | yersinia pseudotuberculosis employs a type iii secretion system for targeting of several virulence factors directly to the cytosol of eukaryotic cells. this protein translocation mechanism mediates the ability of yersinia to resist phagocytosis and is required for sustained extracellular bacterial replication. in the present study, the yersinia outer protein e (yope) was used as a carrier molecule for type iii-dependent secretion and translocation of listeriolysin o (llo) from listeria monocytog ... | 2003 | 12761131 |
| lipopolysaccharides of bacterial pathogens from the genus yersinia: a mini-review. | this review summarizes the state of knowledge on the composition and structure of the lipopolysaccharides (lps) from three species of yersinia known to produce disease in humans: y. pseudotuberculosis, y. enterocolitica and y. pestis. we also mention recent data on the genome sequence of yersinia pestis and the role of lps in relation to the virulence of this bacteria. | 2003 | 12765784 |
| comparison of 16s rdna analysis and rep-pcr genomic fingerprinting for molecular identification of yersinia pseudotuberculosis. | 16s rdna sequence analysis and repetitive element sequence-based pcr (rep-pcr) genomic fingerprinting were evaluated on 11 type strains of the genus yersinia and 17 recognized serotype strains of y. pseudotuberculosis to investigate their genetic relatedness and to establish the value of techniques for the identification of y. pseudotuberculosis. a phylogenetic tree constructed from 16s rdna sequences showed that the type strains of yersinia species formed distinct clusters with the exception of ... | 2003 | 12785306 |
| molecular relatedness between isolates yersinia pseudotuberculosis from a patient and an isolate from mountain spring water. | a 40-yr-old buddhist monk was admitted to the hospital with abdominal pain, fever, and confusion. he had a history of drinking untreated mountain spring water in his temple, and experienced the above symptoms for several days before admission. in past medical history, he had suffered from hepatic cirrhosis. yersinia pseudotuberculosis was isolated from his blood and ascitic fluid. the mountain spring water that he had ingested was cultivated and y. pseudotuberculosis was also isolated. for ident ... | 2003 | 12808333 |
| the erwinia chrysanthemi phop-phoq operon plays an important role in growth at low ph, virulence and bacterial survival in plant tissue. | we have studied the role of acidic ph as a barrier for the colonization of the plant apoplast by erwinia chrysanthemi. a minitransposon containing a promoterless reporter gene, gus, was used for random mutagenesis of the bacterial genome. an acid-sensitive mutant, named bt119, was isolated and had the following differential features with respect to the wild-type strain: (i) inability to grow at ph </= 5.5; (ii) decreased survival at acid ph and in plant tissues; (iii) increased susceptibility to ... | 2003 | 12828634 |