Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| host cell-dependent regulation of growth transformation-associated epstein-barr virus antigens in somatic cell hybrids. | we have analyzed the expression of the three major known growth transformation-associated epstein-barr virus (ebv) proteins, ebna-1, ebna-2, and latent membrane protein (lmp), in a series of somatic cell hybrids derived from the fusion of ebv-carrying burkitt lymphoma (bl) lines with ebv-positive or ebv-negative b-cell lines. independently of the cell phenotype, ebna-1 was invariably coexpressed in all ebv-carrying hybrids. in hybrids between ebv-carrying, lmp-positive and lmp-negative burkitt l ... | 1989 | 2542588 |
| functional limits of orip, the epstein-barr virus plasmid origin of replication. | the epstein-barr virus (ebv) genome contains two cis-acting elements which are required for stable extrachromosomal plasmid maintenance in latently infected cells. the first consists of 20 30-base-pair (bp) repeats, each of which contains a dna-binding site for ebv nuclear antigen 1 (ebna-1), the trans-acting factor required for plasmid persistence. the second element is composed of a 65-bp dyad symmetry, containing four ebna-1-binding sites. deletion mutants were constructed which reduce the nu ... | 1989 | 2542609 |
| responsiveness of the epstein-barr virus noti repeat promoter to the z transactivator is mediated in a cell-type-specific manner by two independent signal regions. | cells latently infected with epstein-barr virus (ebv) can be activated to express lytic-cycle polypeptides by the introduction of the ebv-encoded z transactivator, indicating that this protein has a pivotal role in virus reactivation. we examined the target specificity of the z transactivator in short-term contransfection assays and found that the most responsive target to z transactivation was the divergent noti repeat promoter, located within the ebv bamhi h fragment. in contrast, target plasm ... | 1989 | 2542612 |
| the spliced bzlf1 gene of epstein-barr virus (ebv) transactivates an early ebv promoter and induces the virus productive cycle. | a spliced cdna spanning the epstein-barr virus bzlf1 gene expresses the bzlf1 protein and is active in inducing the virus productive cycle. a deletion mutant which lacks the n-terminal half of the protein is inactive. cotransfection experiments in ebv-negative b-lymphocyte cell lines demonstrated that the bzlf1 gene activates the promoter for the bslf2 + bmlf1 gene in the absence of any other ebv gene product. these results confirmed that the spliced bzlf1 gene is the transactivating gene struct ... | 1989 | 2542618 |
| negative modulation of epstein-barr virus episomes by a human b-cell growth factor. | to study the effect of t-cell-derived bcgf-12kd on human b-cell autocrine growth-associated functions, we cultured epstein barr virus (ebv)-transformed normal b cells (lcl 72285) and burkitt's lymphoma cells (raji) in the presence or absence of bcgf-12kd. when cultured in media supplemented only with fetal calf serum, the lcl and raji cell lines maintained relatively high levels of episomes. although a similar level of proliferation could be maintained under defined culture conditions in media s ... | 1989 | 2542626 |
| [structure and functions of epstein-barr virus genomes]. | 1989 | 2542651 | |
| [dna diagnosis and epidemiologic analysis of epstein-barr virus infections]. | 1989 | 2542655 | |
| [diagnosis of ebv infections using an immunologic method]. | 1989 | 2542659 | |
| [clinicopathological analysis of ebv infection]. | 1989 | 2542669 | |
| [infectious mononucleosis--diagnosis and treatment]. | 1989 | 2542670 | |
| [eb virus-associated tumors-opportunistic b cell lymphoma, burkitt's lymphoma, nasopharyngeal carcinoma]. | 1989 | 2542671 | |
| isolation of human sequences that replicate autonomously in human cells. | we have isolated a heterogeneous collection of human genomic sequences which replicate autonomously when introduced into human cells. the novel strategy for the isolation of these sequences involved cloning random human dna fragments into a defective epstein-barr virus vector. this vector alone was unable to replicate in human cells, but appeared to provide for the nuclear retention of linked dna. the human sequences persist in a long-term replication assay (greater than 2 months) in the presenc ... | 1989 | 2542763 |
| localization of epstein-barr virus dna in the epithelial cells of oral hairy leukoplakia by in situ hybridization of tissue sections. | 1989 | 2542792 | |
| oral hairy leukoplakia: diagnosis and management. | oral hairy leukoplakia (hl) is a remarkable lesion associated with epstein-barr virus (ebv) found in persons infected with the human immunodeficiency virus. the clinical and histologic features of hl are characteristic and distinctive. however, none of these features are entirely specific for hl, and we consider that the presence of ebv is required for diagnosis in questionable cases. ebv can be demonstrated by means of electron microscopy, immunocytochemistry, or molecular biologic techniques. ... | 1989 | 2542857 |
| hairy leukoplakia: an aids-associated opportunistic infection. | hairy leukoplakia has been defined and confirmed as a specific oral mucosal marker for human immunodeficiency virus infection. light microscopic findings include alteration of spinous layer cells, which contain nuclear chromatin abnormalities. enlargement of spinous cells with ballooning degeneration is also characteristic. ultrastructural morphology has defined the presence of large numbers of herpes-type virus particles within the intranuclear, cytoplasmic, and intercellular regions. morpholog ... | 1989 | 2542858 |
| oral hairy leukoplakia with extensive oral mucosal involvement. report of two cases. | oral hairy leukoplakia (hl) is a lesion that occurs predominantly on the tongue in hiv-infected persons. evidence strongly indicates that hl is related to the presence of epstein-barr virus in the epithelial cells. the lesion appears on the lateral border of the tongue as a painless, white plaque varying in size from a few millimeters to extensive lingual involvement. histopathologically, the characteristic findings are hyperparakeratosis, hyperplasia, and ballooning of prickle cells resembling ... | 1989 | 2542859 |
| oral lesions mimicking hairy leukoplakia: a diagnostic dilemma. | oral hairy leukoplakia is seen in immunosuppressed persons infected with the human immunodeficiency virus and is a predictor of the development of acquired immunodeficiency syndrome in that population. over the past 3 years we have seen 16 examples of a lesion that histologically resembles hairy leukoplakia but is found in patients who are not in risk groups for acquired immunodeficiency syndrome. all these specimens tested negative for epstein-barr virus dna and for human papillomavirus antigen ... | 1989 | 2542860 |
| oral lymphomas in hiv-infected patients: association with epstein-barr virus dna. | nine instances of oral non-hodgkin's lymphoma occurring in homosexual male patients infected with the human immunodeficiency virus were evaluated for clinical features, histopathologic features, lymphocyte phenotypic markers, and epstein-barr virus (ebv) dna. histologically, the tumors represented immunoblastic sarcoma and small noncleaved cell lymphomas, some manifesting burkitt-like features. five cases exhibited positive staining with monoclonal antibody l26, a b-cell marker; none of the tumo ... | 1989 | 2542861 |
| why high levels of virus-specific ctl persist in hiv1-infected individuals. | 1989 | 2543042 | |
| epstein-barr virus induced thrombocytopenia treated with intravenous acyclovir and immunoglobulin. | we report infectious mononucleosis in a 15-year-old girl with severe thrombocytopenia (2 x 10(9)/l). intravenous hydrocortisone in high doses for 4 days had no effect. when intravenous immunoglobulin and acyclovir were added an immediate response was noted with normalization of the thrombocyte count within 3 days. | 1989 | 2543058 |
| acute tonsillitis in young men: etiological agents and their differentiation. | group a beta-hemolytic streptococci were found in 38% of 257 young men in military service with acute tonsillitis. of the 108 patients tested also for viral antibodies, 42% showed a 4-fold rise in antibody titers. adenovirus was the most frequent (31%) nonstreptococcal agent, followed by epstein-barr virus (6%), and influenza virus (5%). non-group a streptococci were isolated in about the same proportion (18%) as in healthy control subjects. other data also suggested that these bacteria were car ... | 1989 | 2543062 |
| severe thrombocytopenia in epstein-barr virus-induced mononucleosis. | severe thrombocytopenia is a rare complication of epstein-barr virus-induced infectious mononucleosis. we evaluated the clinical and laboratory data from seven patients seen between 1976 and 1985 whose lowest platelet counts varied from 3 to 25 x 10(9) per liter. five of the seven patients were initially thought to have either acute leukemia or idiopathic thrombocytopenic purpura; eventually, however, primary epstein-barr virus infections were confirmed in all patients. two of six patients teste ... | 1989 | 2543142 |
| fine needle aspiration cytodiagnosis of nasopharyngeal carcinoma in cervical lymph nodes. a study of 40 cases. | the cytologic features of fine needle aspiration (fna) samples of 40 metastatic nasopharyngeal carcinomas in cervical lymph nodes were reviewed. fna was performed with 21-gauge or 23-gauge needles; the fna smears were stained with the papanicolaou stain or with hematoxylin and eosin. several typical cytologic features were noted. all cases showed the presence of clusters of cohesive tumor cells, most of which were undifferentiated. medium-sized oval vesicular nuclei were present in 85% of the ca ... | 1989 | 2543172 |
| detection of epstein-barr virus-associated antigen in fine needle aspiration smears from cervical lymph nodes in the diagnosis of nasopharyngeal carcinoma. | epstein-barr virus (ebv) has consistently been shown to be associated with undifferentiated nasopharyngeal carcinoma, and an ebv-associated nuclear antigen (ebna) has been detected in the cells of nasopharyngeal carcinoma. a study on the applicability of ebna detection in fine needle aspiration (fna) smears from cervical lymph nodes in the diagnosis of metastatic nasopharyngeal carcinoma was performed. all 11 cases (100%) with metastatic nasopharyngeal carcinoma showed ebna-positive tumor cells, ... | 1989 | 2543173 |
| anti-herpes simplex virus activity of 5-substituted 2-pyrimidinone nucleosides. | several 5-substituted 2-pyrimidinone 2'-deoxyribonucleoside (pdr) analogs were examined for their anti-herpes simplex virus (hsv) activity in cell culture. the order of potency of their antiviral activities against hsv type 1 (hsv-1) and hsv-2 was iodo pdr approximately ethynyl pdr approximately propynyl pdr. the antiviral action of iodo pdr is dependent on the ability of hsv to induce virus-specified thymidine kinase in infected cells. several hsv-1 variants with altered thymidine kinase change ... | 1989 | 2543279 |
| assessment of responses of normal human b lymphocytes to different isolates of human immunodeficiency virus: role of normal donor and of cell line used to prepare viral isolate. | the effect of different hiv-1 isolates on normal human b lymphocyte function has been studied in vitro. production of igm and igg was measured by elisa using a "standard" non-t preparation of b cells depleted of macrophages and t cells (but not of low-density accessory cells, ldc). only one (h9/cbl-4) of five different isolates induced polyclonal production of immunoglobulin. apart from intrinsic differences between isolates, important inherent variables were shown to affect the response. one wa ... | 1989 | 2543435 |
| an epstein-barr virus-transformed b cell line produces autoregulatory interleukin-1 that regulates bone remodeling. | in this study, we demonstrate that an epstein-barr virus-transformed b cell line, a-11, produced interleukin-1 (il-1), a cytokine that regulates bone remodeling. a-11 cells produce il-1 in a cell dose- and culture time-related manner. the il-1 activity was neutralized by recombinant human il-1 (rhil-1) alpha antiserum, but not by rhil-1 beta antiserum. the il-1 was semi-purified by (nh4)2so4 precipitation, superose prep 12 gel filtration, and anion-exchange chromatography strongly stimulated in ... | 1989 | 2543455 |
| glycosylation of human leukocyte locus a molecules is dependent on the cell type. | peripheral blood monocytes and b cells were isolated from a normal donor, and a portion of the b cells was transformed by the epstein-barr virus (ebv). human leukocyte locus a (hla) class-i and class-ii molecules were immunoprecipitated by specific monoclonal antibodies after cell labeling with [3h]mannose. glycopeptides of hla molecules were obtained by pronase digestion and were analysed by lectin-affinity chromatography. complex structures were hydrazinolysed, and their sialic acid content wa ... | 1989 | 2543568 |
| [an association between hepatitis-b antigen-negative infantile papular acrodermatitis and epstein-barr virus infection]. | the virological studies on 23 patients with infantile papular acrodermatitis (ipa) without hepatitis b virus (hbv)-associated antigens and antibodies were performed. the following results were obtained; 1) there was serological evidence of primary epstein-barr virus (ebv) infection in 17 out of 23 cases (74%). 2) the regression assays was done in 5 cases of ipa and 10 cases of infectious mononucleosis known to be associated with primary ebv infection in order to investigate the development of eb ... | 1989 | 2543617 |
| human ige response: virus-activated ige secretors are interleukin-2-dependent cells. | the activation of human ige-secreting b lymphocytes was difficult until the use of low cell density limiting dilution cultures, which are more efficient and eliminate regulatory growth restraints, became possible. the transformation of ige-producing cells by epstein-barr virus unexpectedly required the presence of small supplements of mature t lymphocytes early in culture. interleukin 2 (il2, 10 u/ml) was able to completely replace t-cell supplements when added during a brief (1-2 cell cycles) b ... | 1989 | 2543638 |
| relationship between clinical stage, histopathology and antibody titers against the second epstein-barr virus nuclear antigen (ebna-2) in non-hodgkin's lymphoma patients. | non-hodgkin lymphoma (nhl) patients with centroblastic (cb) or centroblastic-centrocytic (cb/cc)-diffuse lymphomas, immunocytoma (ic) and chronic lymphocytic leukemia (cll) in clinical stages iii-iv and with active disease (highly malignant group) were compared to nhl patients with cll, ic, and centrocytic (cc) or centroblastic-centrocytic (cb-cc)-diffuse/follicular lymphomas, in clinical stages i-ii and with inactive disease (low malignant group) based on the presence of antibodies to epstein-b ... | 1989 | 2543639 |
| induction of epstein-barr virus in b-lymphoblastoid cells by human immunodeficiency virus type 1. | individuals infected with the human immunodeficiency virus (hiv), the etiologic agent of acquired immunodeficiency syndrome (aids), often show symptoms associated with reactivation of epstein-barr virus (ebv). in this study, we show that exposure of ebv-positive b lymphocytes to hiv-1 in vitro induced the ebv replicative cycle in these cells, as evidenced by an increased proportion of cells expressing ebv early antigens (ea) and capsid antigens (vca). reactivation of ebv by hiv-1 appeared to be ... | 1989 | 2543643 |
| susceptibility to azt of hiv-1 variants grown in epstein-barr virus-transformed b cell lines. | how 3'-azido-3'-deoxythymidine (azt) affects the ability of three different variants of hiv-1 to infect a line of ebv-transformed b cells was studied. susceptible cells were pretreated with three different concentrations of azt (1, 5, and 10 microm) for 4 h before viral inoculation and were maintained after infection in drug-containing medium. establishment of viral infection was assessed by indirect immunofluorescence for viral antigens and by antigen capture assay carried out on culture fluids ... | 1989 | 2543710 |
| detection of epstein-barr virus dna by polymerase chain reaction in blood and tissue biopsies from patients with sjogren's syndrome. | polymerase chain reaction has been used to detect increased levels of ebv dna in salivary gland (sg) biopsies and pbl from patients with sjogren's syndrome (ss). these results suggest that ebv, which has a normal site of latency in a small number of sg epithelial cells, may be reactivated in ss patients and provide a target for immune attack. the great sensitivity of polymerase chain reaction (pcr) and the ability to analyze very small tissue biopsies (37) make this technique well suited for cli ... | 1989 | 2543732 |
| identification and nucleotide sequence of a gene in equine herpesvirus 1 analogous to the herpes simplex virus gene encoding the major envelope glycoprotein gb. | a gene in equine herpesvirus 1 (ehv-1; equine abortion virus) equivalent to the gb glycoprotein gene of herpes simplex virus (hsv) has been identified by dna hybridization and nucleotide sequencing. a 4.3 kbp ehv-1 psti-clai sequence (0.40 to 0.43 map units) contained an open reading frame flanked by appropriate control elements and was capable of encoding a polypeptide of 980 amino acids. this had 50 to 60% identity over a 617 amino acid conserved region with the gb gene products of hsv and thr ... | 1989 | 2543744 |
| epstein-barr virus with transforming and early antigen-inducing ability originating from nasopharyngeal carcinoma: mapping of the viral genome. | epstein-barr virus (ebv) from a human hybrid epithelial cell line (npc-kt), derived from the fusion of human adenoidal cells and ebv genome-containing primary nasopharyngeal carcinoma cells (npc) has both transforming and early antigen (ea)-inducing abilities. ebv dna from npc-kt cells was partially digested with taqi and ligated to the cloning vector pjb8 at the clai site. this cosmid library encompassed the whole genomic dna of the virus except for several kb of the terminal fragments. the ide ... | 1989 | 2543756 |
| expression of epstein-barr virus membrane antigen gp340/220 in mouse fibroblasts using a bovine papillomavirus vector. | epstein-barr virus (ebv) membrane antigen glycoproteins gp340 and gp220 are encoded by a single gene. we have inserted this gene into a bovine papillomavirus (bpv) vector and expressed gp340/220 in mammalian cells under the control of the mouse metallothionein promoter. the proteins produced were of similar mr, showed similar antigenic specificity and were transported to the same subcellular location as the authentic gp340/220. the inclusion of heavy metal ions in the medium had no effect on the ... | 1989 | 2543757 |
| epstein-barr virus dna sequences in precursor monocyte-macrophage cell lines established from the bone marrow of children with maturation defects of haematopoiesis. | epstein-barr virus (ebv) dna sequences were detected in four established monoblast or early monocytic cell lines (cm-s, rov-s, cv-s and ad-s) obtained from bone marrow of children suffering from maturation defects of haematopoiesis. ebv is present in these cells in a latent state. the viral dna in these cell lines was analysed by southern blot hybridization, using a set of cloned ebv dna fragments from the ebv strain b95-8 as probes. a common spectrum of highly related but distinguishable ebv dn ... | 1989 | 2543775 |
| enhanced expression of the epstein-barr virus latent membrane protein by a recombinant vaccinia virus. | the complete coding sequence of the epstein-barr virus strain b95-8 latent membrane protein (lmp) was cloned using a raji cell cdna library and genomic b95-8 dna. the clone was characterized by sequencing and then used to make a recombinant vaccinia virus. this virus (vlmp) was shown to express a relatively high level of lmp in an authentic fashion. antisera raised in rabbits against vlmp were shown to react with b95-8 lmp as well as cross-reacting with a 50k cellular protein. | 1989 | 2543776 |
| [studies on the levels of specific antibodies to epstein-barr virus and heterophile antibody in the patients with infection mononucleosis]. | 1989 | 2543850 | |
| growth- and tumor-promoting effects of deregulated bcl2 in human b-lymphoblastoid cells. | human follicular b-cell lymphomas possess a t(14;18) that translocates a putative protooncogene, bcl2, into the immunoglobulin heavy chain locus. the normal bcl2 gene is quiescent in resting b cells, expressed in proliferating, but down-regulated in differentiated b cells. inappropriately high levels of bcl2-immunoglobulin chimeric rna are present in t(14;18) lymphomas for their mature b-cell stage. we examined the biologic effects of bcl2 deregulation in human b cells by introducing bcl2 into h ... | 1989 | 2543982 |
| [interleukin-1-like factor from human b-lymphocytes transformed by the epstein-barr virus]. | the nc-37, ebv-transformed human b-lymphoblastoid cell line spontaneously produces interleukin (il)-1-like factor, which is able to stimulate proliferation of mouse thymocytes and human fibroblasts. the il-1-like factor production can be enhanced by prodigiozan and by the conditioned medium of human peripheral blood mononuclear cells. the nc-37 cells have a membrane-associated form of il-1-like factor also. the molecular mass of the intracellular precursor of this factor determined by gel-filtra ... | 1989 | 2544049 |
| [primary sjögren's syndrome complicated by gastric lymphoma. study of epstein-barr viral dna in the tumor]. | 1989 | 2544120 | |
| the immune system as mediator of virus-associated bone marrow failure: b19 parvovirus and epstein-barr virus. | 1989 | 2544123 | |
| a monocyte-derived b cell growth factor is ifn-beta 2/bsf-2/il-6. | 1989 | 2544130 | |
| normal serologic response to epstein-barr virus in patients with sjögren's syndrome. | 1989 | 2544190 | |
| interaction of hiv and ebv at lymphoid tissue level: immunohistochemistry and in situ hybridization. | the presence of epstein-barr virus antigens and genome was studied in lymph nodes from hiv + patients affected by pgl. cryostat sections from 50 lymph nodes of hiv + patients were immunostained with ebv-vca (viral capsid antigen), ebv-ea (early antigen) and p24 hiv major core protein monoclonal antibodies. in situ hybridization was performed using a biotin conjugated ebv dna probe; the reaction product was demonstrated by immunohistochemical method. as positive controls, ebv producer b95-8 and h ... | 1989 | 2544215 |
| the role of epstein-barr virus in lymphomas of hiv-carriers. | a substantial proportion of the lymphomas in hiv-carriers are ebv positive. together with the fact that there are multiple signs of ebv-activation in aids-patients and patients with arc or pgl, this suggests that these virus carrying tumors develop as results of the immunosuppression, and that ebv has an important role in their pathogenesis. | 1989 | 2544216 |
| [chronic fatigue syndrome]. | 1989 | 2544272 | |
| repair of 8-methoxypsoralen + uva-induced damage in specific sequences in chromosomal and episomal dna in human cells. | a study of the repair of dna damage in the dihydrofolate reductase (dhfr) gene of sv40-transformed human fibroblasts after treatment with 8-methoxypsoralen (8mop) and uva is described. 8mop+uva-induced cross-links in the dhfr gene were completely repaired by 12 h in one normal and one fanconi's anaemia (fa) group a cell line. in contrast, approximately 35% of cross-links in an episomally maintained epstein--barr virus derived plasmid remained unrepaired even after 48 h. cross-linkable monoadduct ... | 1989 | 2544311 |
| chronic infection with epstein-barr virus, chlamydia and hepatitis a virus, terminating in cirrhosis and nasopharyngeal carcinoma. | we describe a 27-year-old woman who presented with infertility, and over the subsequent 7 years developed chronic epstein-barr virus infection, hepatitis a, cirrhosis and nasopharyngeal carcinoma. serological evidence of chronic infection with chlamydia, epstein-barr virus and hepatitis a virus was documented. | 1989 | 2544539 |
| effect of phosphorothioate homo-oligodeoxynucleotides on herpes simplex virus type 2-induced dna polymerase. | effects of phosphorothioate oligodeoxynucleotides of different chain length and base composition on herpes simplex virus (hsv) type 2 (strain 333)-induced dna polymerase have been examined in vitro. the anti-hsv-2 dna polymerase activity was related to the base composition of the analogs, with the order of potency: deoxycytidine greater than thymidine greater than deoxyadenosine, for compounds with equal chain length. the potency was also related to oligomer chain length, since it was observed t ... | 1989 | 2544598 |
| cd3-negative lymphoproliferative disease of granular lymphocytes containing epstein-barr viral dna. | lymphoproliferative disease of granular lymphocytes (ldgl) is a heterogeneous disorder and the pathogenesis is likely to be complex. some patients with chronic active ebv (caebv) infection also have ldgl. to investigate the relationship between ebv infection and the pathogenesis of ldgl, we conducted a survey for ebv dna sequences by southern blot analysis of dna obtained from the peripheral blood of seven patients with ldgl, including one with caebv infection. interestingly, ebv dna was detecte ... | 1989 | 2544630 |
| epstein-barr virus latent gene expression during the initiation of b cell immortalization. | epstein-barr virus (ebv) has the capacity to immortalize a subpopulation of resting b lymphocytes. lymphoblastoid cell lines (lcl) established in this way carry the latent ebv genome as multiple copies of an extrachromosomal episome. viral gene expression in lcls is highly restricted; products identified correspond to a membrane protein (latent membrane protein; lmp), a nuclear antigen complex (epstein-barr nuclear antigens; ebnas 1 to 6), two small rna species (ebers 1 and 2) and rna species th ... | 1989 | 2544663 |
| nucleotide sequence and characterization of the marek's disease virus homologue of glycoprotein b of herpes simplex virus. | the marek's disease virus (mdv) homologue of the herpes simplex virus (hsv) gene encoding glycoprotein b (gb) has been identified within bamhi fragments i3 and k3 of the 'highly oncogenic' strain rb1b of mdv. the entire nucleotide sequence of the gene has been determined and its predicted amino acid sequence shown to share gross overall structural features with the gb genes of hsv, varicella-zoster virus (vzv) and other mammalian herpesviruses. in particular, all 10 cysteine residues were conser ... | 1989 | 2544666 |
| antibody against epstein-barr virus dna polymerase activity in sera of patients with nasopharyngeal carcinoma. | a salt-dependent dna polymerase activity was demonstrated in the culture of an ebv-producing, lymphoblastoid cell line (npc-204 cells) treated with 5-iodo-2'-deoxyuridine (iudr). there was a high frequency of levels of antibody to this enzyme in sera of patients with nasopharyngeal carcinoma (npc). in contrast, sera from healthy subjects had little or no neutralizing activity. the high antibody level appeared as early as stage 1 of the disease in many npc patients. the levels of the antibody inc ... | 1989 | 2544674 |
| brief report: killer cell defect and persistent immunological abnormalities in two patients with chronic active epstein-barr virus infection. | two members of a family have manifested a syndrome of chronic active epstein-barr virus (ebv) infection. a father and his daughter suffered prolonged or recurrent mononucleosis, with splenomegaly, anemia, and intermittent fever; persistent immunological abnormalities included defective natural killer (nk) cytotoxicity, inverted cd4/cd8 ratios, hyper igg1, high ebv viral capsid antigen (vca) and early antigen (ea) antibodies, and low or undetectable ebv nuclear antigen (ebna) antibody titers. the ... | 1989 | 2544675 |
| human herpesvirus epstein-bar virus: ubiquity, oncogenicity, and risk factors. | 1989 | 2544754 | |
| [vascular purpura and other cardiovascular alterations in children with acute parvovirus b19 infection. report of 3 personal cases]. | most frequent skin manifestation of parvovirus b19 infection is the erythema infectiosum (fifth disease; sticker's disease). vascular purpura (some cases of henoch-schönlein purpura) has been reported in virus infections such as infectious mononucleosis (epstein-barr virus), hepatitis b, cytomegalovirus etc., and human parvovirus b19 could be another cause. clinical course, procedure of diagnostics and therapy in 3 own serologically established cases of vascular purpura or severe alterations of ... | 1989 | 2544763 |
| epstein-barr-virus-associated lymphoproliferation arising in a renal allograft. | 1989 | 2544819 | |
| [meningoencephalitis in epstein-barr virus reactivation in a renal transplant patient]. | 1989 | 2544870 | |
| human diabetes associated with a deletion of the tyrosine kinase domain of the insulin receptor. | the insulin receptor has an intrinsic tyrosine kinase activity that is essential for signal transduction. a mutant insulin receptor gene lacking almost the entire kinase domain has been identified in an individual with type a insulin resistance and acanthosis nigricans. insulin binding to the erythrocytes or cultured fibroblasts from this individual was normal. however receptor autophosphorylation and tyrosine kinase activity toward an exogenous substrate were reduced in partially purified insul ... | 1989 | 2544997 |
| chronic fatigue syndrome. a critical appraisal of the role of epstein-barr virus. | the symptom complex currently designated the chronic fatigue syndrome was previously termed the chronic or chronic active epstein-barr virus syndrome or the chronic mononucleosis syndrome, prematurely assuming an etiologic role for the epstein-barr virus (ebv). this presumption derived from the fact that some patients with the chronic fatigue syndrome have very high or very low titers of certain antibodies to ebv. a review of seroepidemiologic patterns of response to ebv and of studies of patien ... | 1989 | 2545048 |
| diffuse large-cell lymphoma of b-cell origin and deficient t-cell function in a patient with rheumatoid arthritis. | an increased incidence of non-hodgkin's lymphoma has been described in patients with rheumatoid arthritis. mechanisms related to abnormal immune regulation have been postulated, but no patients with rheumatoid arthritis and lymphoma have been previously well characterized immunologically. we describe here a patient with long-standing rheumatoid arthritis in whom a b-cell diffuse large-cell lymphoma developed. he was found to have a severe t-cell immunodeficiency and evidence of persistent epstei ... | 1989 | 2545174 |
| human monoclonal antibodies to influenza virus: igg subclass and light chain distribution. | three adults and three children were immunized with inactivated or live attenuated influenza vaccines and 98 igg monoclonal antibodies derived from ebv immortalization of their blood lymphocytes were studied. all antibodies reacted specifically with influenza a h3n2 or h1n1 whole virus and 73 of 74 tested reacted with the purified ha glycoproteins. the majority (76%) of 77 monoclonal antibodies adequately tested by elisa or solid phase ria contained lambda light chains. elisa analysis of the igg ... | 1989 | 2545224 |
| the hodgkin-associated ki-1 antigen exists in an intracellular and a membrane-bound form. | the hodgkin-associated ki-1 antigen occurs in two different molecular forms. the 120-kda membrane-associated form is a phosphorylated glycoprotein, which is derived from a non-phosphorylated intracellular 84-kda apoprotein that is co-translationally n-glycosylated with a carbohydrate portion of 6 kda. the other form of the ki-1 antigen is a non-glycosylated phosphoprotein of 57 kda which only occurs intracellularly. both forms of the antigen are phosphorylated at serine residues. enzymatic cleav ... | 1989 | 2545229 |
| epstein-barr virus: the hematologic and oncologic consequences of virus-host interaction. | varicella-zoster virus (vzv) and epstein-barr virus (ebv) are two of the human herpesviruses. the others include herpes simplex virus (hsv) type 1, hsv type 2, and cytomegalovirus (cmv). in a series of two articles, we review the clinical diseases caused by vzv and ebv infections; we pay particular attention to the manifestations of these two viral infections in immunosuppressed and immunocompromised patients. in addition to the clinical reviews, each of the two articles begins with a brief disc ... | 1989 | 2545365 |
| [a case of recurrent epstein-barr virus meningitis]. | a 17-year-old male was admitted to our hospital complaining of fever, headache and nausea. on physical examinations cervical and inguinal lymphadenopathy and hepatosplenomegaly were noted. neurological examination revealed meningeal signs. blood examination showed slightly increased lymphocytes and atypical lymphocytes (1-4%), increased esr, crp (2+), slightly elevated ebv vca igg (x20), and normal ebv vca igm (less than x 10). initial pressure of csf was 195mmh2o, cells 46/3mm3, protein 50mg/dl ... | 1989 | 2545400 |
| [a case of meningoencephalitis caused by persistent epstein-barr (e-b) virus infection]. | a 44-year-old man had several episodes of tingling sensation in his lower extremities, head and face over 1 year and presented mental disturbance 2 months prior to admission. he additionally suffered from dysuria and acute onset of gait disturbance 18 days before admission. physical and neurological examination revealed marked splenomegaly, stupor, abnormal behavior, spastic tetraparesis and sphincter disturbances, and meningoencephalitis was suspected. there was lymphocytosis in peripheral bloo ... | 1989 | 2545401 |
| herpesvirus infections in the immunocompromised host. clinical spectrum, diagnosis, management and prophylaxis. | 1989 | 2545413 | |
| reversion of tumorigenicity in an ebv-converted burkitt's lymphoma line. | one of five epstein-barr virus (ebv)-converted sublines of an ebv-negative burkitt's lymphoma line (bl-41) was identified as a non-tumorigenic phenotypic revertant with low clonability, comparable to that of an ebv-transformed lymphoblastoid cell line (iarc-171) derived from the same patient. this revertant subline (bl-41/95) also showed the most lcl-like phenotype of the five convertants tested. it is suggested that reversion was due to the phenotypic shift of the cell from a 'window' of cell o ... | 1989 | 2545422 |
| construction of an ebna-producing line of well-differentiated human hepatoma cells and of appropriate epstein-barr virus-based shuttle vectors. | using cloned epstein-barr nuclear antigen 1 (ebna) and orip elements from the epstein-barr virus (ebv) in conjunction with liver-specific growth media, we have constructed an ebna-producing line of well-differentiated human hepatoma cells (hep-ebna-2) and appropriate ebv-orip vectors. these vectors, pbedc1 and pbeug1, were maintained as free extrachromosomal elements only in cells that expressed the trans-acting ebna protein. they were readily rescued from transfected hep-ebna-2 cells upon trans ... | 1989 | 2545532 |
| identification of epstein-barr virus-infected cells in tonsils of acute infectious mononucleosis by in situ hybridization. | in situ hybridization with 35s-labeled epstein-barr virus (ebv) probes was applied to paraffin sections of tonsils from seven patients with clinical, serologic, and morphologic evidence of acute infectious mononucleosis. ebv genomes were demonstrated in activated lymphoid b blasts in the interfollicular and perifollicular zones in all these cases. however, in no case could ebv be identified in epithelial cells. these results are at variance with the current concept which attributes a central rol ... | 1989 | 2545594 |
| the transforming prototype of epstein-barr virus (b95-8) is also a lytic virus. | the b95-8 isolate of the epstein-barr virus (ebv) has been described as a non-lytic transforming virus. we have performed experiments in order to determine if the b95-8 ebv is capable of super-infecting and replicating in ebv-genome-positive non-producer lymphoblastoid cells. using concentrates of b95-8 ebv, prepared from 6 different b95-8 cell lines treated with 12-o-tetradecanoylphorbol-13-acetate (tpa), we demonstrated that virus concentrates could transform human or cotton-top tamarin b-lymp ... | 1989 | 2545637 |
| rapid diagnosis of acute epstein-barr virus infection by an indirect enzyme-linked immunosorbent assay for specific immunoglobulin m (igm) antibody without rheumatoid factor and specific igg interference. | an indirect enzyme-linked immunosorbent assay (elisa) for detection of epstein-barr virus-specific immunoglobulin m (igm) antibody was developed with commercial reagents. sera containing rheumatoid factor (rf) (as little as 0.5 iu/ml) coupled with specific igg resulted in false-positives in the elisa. this interference was eliminated by the use of anti-human igg antibodies to remove rf and igg. thus, pathogen-specific igg complexes to which igm-rf could be bound during the subsequent test were i ... | 1989 | 2545744 |
| mr features of fleeting cns lesions associated with epstein-barr virus infection. | the magnetic resonance and ct findings in a 10-year-old patient with neurologic abnormalities associated with epstein-barr virus infection are reported. multiple focal lesions of various sizes were demonstrated with involvement at one time or another of both cerebral hemispheres, the right thalamus, the right cerebellar hemisphere, and the right optic nerve. a unique finding was the fleeting nature of the lesions with new lesions appearing as others resolved. the clinical presentation, laborator ... | 1989 | 2545753 |
| recombinant interferon-gamma in the treatment of recurrent nasopharyngeal carcinoma. | the etiologic relationship of the epstein-barr virus (ebv) to nasopharyngeal carcinoma prompted this study of the efficacy of interferon-gamma (ifn-gamma), which possesses antiviral and antitumor activity, in the treatment of recurrent carcinoma. fourteen patients received recombinant ifn-gamma in daily intramuscular doses of 5-10 x 10(6) u/m2. of 13 patients evaluable for response, five had a minor response; disease was stable in two and had progressed in six. ebv serology for immunoglobin (ig) ... | 1989 | 2545832 |
| [suppressive effect of cddp on induction of the epstein-barr virus antigen synthesis by n-butyrate]. | n-butyrate has been shown to induce epstein-barr virus (ebv) antigen synthesis in certain ebv genome-carrying lymphoblastoid cell lines. we have studied the effect of cis-dichloro-diamine-platinum (cddp) on induction of ebv antigen synthesis not only by n-butyrate treatment of the ebv producer, p3hr-1 cells, but also by superinfection of ebv to the ebv non-producer, raji cells. simultaneous treatment of the cells with n-butyrate and cddp or mmc blocked vca and ea induction, but no suppressive ef ... | 1989 | 2545850 |
| oral hairy leukoplakia is not a specific sign of hiv-infection but related to immunosuppression in general. | oral hairy leukoplakia (hl) has been regarded as an early sign of hiv infection, and its clinical importance related to the poor outcome of the patients has been emphasized. initially, hl was observed exclusively among male homosexuals, but subsequently demonstrated in all risk groups of hiv infection. the patient described in this article suggests that oral hl is not specific for hiv infection per se, but may be associated with immunosuppression also due to other causes. we describe an hiv-sero ... | 1989 | 2545869 |
| oral hairy leukoplakia in an hiv-negative renal transplant recipient. | oral hairy leukoplakia (hl) has been seen exclusively in those infected with hiv or at risk for aids. this case report describes an example of hl seen in a renal transplant recipient who was negative for hiv on serology and culture. the diagnosis of hl was confirmed using in situ hybridization for ebv dna. | 1989 | 2545870 |
| oral manifestations of hiv infection in 75 dutch patients. | seventy-five consecutive hiv-infected patients, including 33 aids cases were examined. one or more oral mucosal lesions were observed in 57 (76%); candidiasis was the most common finding (52%). others included hairy leukoplakia (16%), periodontal disease (16%) and kaposi's sarcoma (4%). diversity of study design and methods allowed no reliable comparison with other reports. | 1989 | 2545871 |
| lymphomatoid granulomatosis in a 13-month-old infant. | lymphomatoid granulomatosis is an infrequent, progressive, and frequently fatal vasculitis which typically occurs in middle aged males. we describe a 13-month-old infant with lymphomatoid granulomatosis who presented with chronic otitis media and "failure to thrive." although it is well recognized that rheumatic conditions may result in failure to thrive, pediatricians often fail to consider this possibility and the proper diagnosis and treatment are correspondingly delayed. lymphomatoid granulo ... | 1989 | 2545878 |
| deletion mutants of herpesvirus saimiri define an open reading frame necessary for transformation. | analysis of a 5,549-base-pair sequence at the left end of herpesvirus saimiri unique (l-) dna revealed two open reading frames and genes for five small nuclear u rnas (herpesvirus saimiri u rnas). replication-competent deletion mutants were constructed in order to assess the importance of these genetic features for transformation by this oncogenic herpesvirus. although not required for replication, one of the open reading frames was found to be required for immortalization of marmoset t lymphocy ... | 1989 | 2545905 |
| induction of anti-ebna-1 protein by 12-o-tetradecanoylphorbol-13-acetate treatment of human lymphoblastoid cells. | binding of the epstein-barr virus (ebv) nuclear antigen (ebna-1) to bamhi-c dna was studied by affinity column chromatography followed by immunoblotting with human serum specific for ebna-1. two species of ebna-1 (68 and 70 kilodaltons) were identified in nuclear extracts of the ebv-positive burkitt's lymphoma cell line raji and not in nuclear extracts of the ebv-negative burkitt's lymphoma cell line bjab. both ebna-1s bound specifically to the region required for ebv plasmid dna maintenance (or ... | 1989 | 2545906 |
| cytopathic effects induced by epstein-barr virus replication in epithelial nasopharyngeal carcinoma hybrid cells. | npc-kt cl.s61, a subclone derived from an epithelial-nasopharyngeal carcinoma hybrid cell line (npc-kt), showed cytopathic changes characteristic of herpesvirus replication, including formation of multinucleated giant cells and inclusion bodies, when epstein-barr virus replicative cycle was induced by 5-iodo-2'-deoxyuridine. acyclovir (an inhibitor of herpesvirus dna polymerase), epstein-barr virus-immune human serum, or 2-deoxyglucose (an inhibitor of the glycosylation) interfered with syncytiu ... | 1989 | 2545926 |
| [translocation (8; 22) (q24; q11) and epstein-barr viral genome in a case of burkitt's type of acute lymphoblastic leukemia (l3)]. | a case of burkitt's type of acute lymphoblastic leukemia (fab l3) in an 80-year-old male patient is reported. at the time of diagnosis, 74% of the bone marrow cells were lymphoblastoid cells, which were large and homogeneous in size, and whose cytoplasm was abundant and intensely basophilic containing many vacuoles. lambda-light chains were detected as surface immunoglobulin (ig), but not heavy chains or kappa-light chains. epstein-barr (eb) viral genome was detected in the cultured bone marrow ... | 1989 | 2545941 |
| production of human heterophile antibodies. iii. in vitro specific stimulation and immortalization of human lymphocytes. | the production of primary human specific antibody against an exogenous antigen by in vitro system was achieved in this study. human lymphocytes were prepared from the lymphocytes of human spleen (spl), tonsil (tl), and peripheral blood (pbl). these lymphocytes were stimulated by sheep red blood cells (srbc) co-cultured with the appropriate number of allogeneic lymphocytes or fractionated t cells. significant numbers of plaque forming cells (pfc) against srbc were obtained. a major population of ... | 1989 | 2545960 |
| [chronic fatigue syndrome associated with epstein-barr virus infection]. | epstein-barr virus (ebv) infection is ubiquitous and may result in multiple and widely different clinical features; the most common of these is infectious mononucleosis (im). recently, a group of patients has been included in the chronic ebv infection syndrome (ebvis), with a sustained nonspecific syndrome consisting of asthenia, anorexia, low grade fever and changes in mood, associated with a viral infection not necessarily caused by ebv; this has been called chronic fatigue syndrome (cfs). we ... | 1989 | 2545980 |
| further studies on growth characteristics of epstein-barr virus (ebv) genome-positive and -negative epithelial hybrid cell lines. | differences in tumorigenicity between the epstein-barr virus (ebv) genome-negative and -positive epithelial a2l/ah hybrid cell lines have been described recently. further differences in growth characteristics of the two epithelial hybrid cells have since been examined. differences were found in doubling time and cloning efficiency in agarose of the two cell lines. in addition, the ebv genome-negative epithelial hybrid cell line (cl-654), unlike the ebv genome-positive epithelial hybrid cell line ... | 1989 | 2545984 |
| ige secretion by epstein-barr virus-infected purified human b lymphocytes is stimulated by interleukin 4 and suppressed by interferon gamma. | the cytokine interleukin 4 (il-4) has been shown to induce lipopolysaccharide-activated murine b cells to differentiate into ige-secreting cells and to stimulate ige secretion by cultured human peripheral blood lymphoid cells. it is unclear, however, whether this effect of il-4 on human peripheral blood lymphoid cells is a direct effect on the b cell because il-4 can stimulate t cells and monocytes as well as b cells and does not induce purified human b cells to secrete immunoglobulin. to invest ... | 1989 | 2546158 |
| pulmonary infection in human immunodeficiency disease: viral pulmonary infections. | viral pneumonitides are among the known pulmonary complications of human immunodeficiency virus (hiv) infection. cytomegalovirus (cmv) pneumonitis is the most frequently recognized viral infection involving the lung. although cmv may occasionally be the sole pathogen found to be responsible for severe pneumonitis in patients with the acquired immunodeficiency syndrome (aids), in most cases, its role in causing pulmonary disease is less clear, primarily because of the propensity to infect with a ... | 1989 | 2546236 |
| transmission of epstein-barr virus by a transplanted kidney, with activation by okt3 antibody. | 1989 | 2546300 | |
| analysis of lymphoepithelioma and lymphoepithelioma-like carcinomas for epstein-barr viral genomes by in situ hybridization. | lymphoepithelioma of the nasopharynx has a strong association with epstein-barr virus (ebv). to test the hypothesis that lymphoepithelioma-like carcinomas occurring at other sites are also associated with ebv virus, we used in situ hybridization to analyze 20 cases of lymphoepithelioma and histologically similar lesions and five basaloid squamous cell carcinomas for evidence of ebv genomes. ebv genomes were demonstrated in six of six lymphoepitheliomas of the nasopharynx but in none of five basa ... | 1989 | 2546458 |
| lymphoepithelioma-like carcinoma of the lung. | originally described in the nasopharynx, lymphoepitheliomas are undifferentiated carcinomas with prominent lymphoid infiltration. recently this tumor has been described in a number of other sites. an association with prior infection by the epstein-barr virus has been recognized in cases from the nasopharynx. we report four cases of lymphoepithelioma-like carcinoma arising in the lung. epstein-barr virus serology indicated prior infection in two cases, one of which was also found by in situ hybri ... | 1989 | 2546459 |
| acquired immune deficiency, myelodysplasia, and acute nonlymphocytic leukemia associated with monosomy 7 and t(3;3) (q21;q26) in a child with langerhans cell histiocytosis. | a case of therapy-related myelodysplasia followed by acute nonlymphocytic leukemia in a 5-year-old child successfully treated for diffuse langerhans cell histiocytosis is described. a syndrome of severe cell-mediated immune deficiency and persistent epstein-barr virus (ebv) infection coincided with the evolution of myelodysplasia. specific abnormalities of chromosomes number 7 and 3 were associated with the onset of myelodysplasia and acute nonlymphocytic leukemia and are believed to be linked t ... | 1989 | 2546462 |
| establishment and characterization of an epstein-barr virus transformed cell line with strong phagocytic activity. | an immunoglobulin m (igm)-positive cell line, ms 28, apparently spontaneously transformed by epstein-barr virus (ebv) was established from peripheral blood cells of a patient with immature myeloblastic leukemia. it has been characterized according to phenotype, cytochemistry, and membrane antigen pattern. the cell line expresses lymphoid markers like cd 19, cd 22, and cd 30 and synthesizes and secretes igm. monocyte markers cd 11c, cd 14, and cd 15 are absent. neither interleukin-1 (il-1), nor t ... | 1989 | 2546624 |
| demonstration of monoclonal ebv genomes in hodgkin's disease and ki-1-positive anaplastic large cell lymphoma by combined southern blot and in situ hybridization. | forty-two cases of hodgkin's disease (hd) and 22 cases of ki-1-positive anaplastic large cell (ki-1 + alc) lymphoma were examined by southern blotting for the presence of epstein-barr virus (ebv) dna. seven cases of hd and one case of ki-1 + alc lymphoma scored positive with a probe specific for the internal repetitive region of the ebv genome. subsequently, these viral genomes could be demonstrated to be monoclonal in origin using an ebv terminal region dna probe. in situ hybridization revealed ... | 1989 | 2546633 |
| macrophage colony-stimulating factor (csf-1) is expressed by spontaneously outgrown ebv-b cell lines and activated normal b lymphocytes. | human b lymphocytes activated by mitogens or infected by epstein barr virus (ebv) have previously been shown to release colony-stimulating activity (csa) supporting the growth of normal human bone marrow progenitors. we established five different human ebv-b cell lines spontaneously outgrown from nonmalignant peripheral blood cells and long-term bone marrow cultures. csa derived from all of these lines induces the growth of murine macrophage colonies, whereas virtually no human bone marrow cell ... | 1989 | 2546636 |
| viral latency and transformation: the strategy of epstein-barr virus. | 1989 | 2546674 | |
| mechanisms other than polyclonal b cell activation possibly involved in epstein-barr virus-induced autoimmunity. | in order to verify whether epstein-barr virus (ebv)-induced polyclonal b cell activation is the major cause of autoimmunity during infectious mononucleosis (im), we have investigated, by immunoblotting, the fine specificity of anti-smooth muscle autoantibodies (autoabs) in the sera of im patients. furthermore, we have isolated a number of in vivo infected ebv-positive cell lines from a patient with im and compared the reactivity of the secreted immunoglobulins (igs) with that of serum autoabs. t ... | 1989 | 2546702 |