Publications

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[on data on the amidation of nucleotide- and isopropanolamine-free b 12 polycarboxylic acids by propionibacterium shermanue]. 196514287720
separation of proteins from propionibacteria on cellulose ion exchangers. 196514302411
[the iosynthesis of b-12 factors v-1a and b from nucleotide-free b-12 polycarboxylic acids using propionibacterium shermanii]. 196414317976
[gc percentage and classification. application to the bifidobacteria group and some related genera]. 196514336046
[biosynthesis of various b-12 analogs fluorinated in the alkanolamine portion using bropionibacterium shermanii]. 196414339647
the lactic dehydrogenase of propionibacterium pentosaceum. 196014423361
biosynthesis of cobalamins by cell suspensions of propionibacteria and streptomycetes. 195914431889
[biosynthesis of vitamin b12 porphyrins by a culture of propionic bacteria]. 196014435057
[contribution to the biosynthesis of cyanocobalamine in propionibacterium shermanii cultures]. 195914446894
[on the enrichment of kefir with vitamin b12 by the addition of a microbial association of propionibacterium shermanii]. 196114453982
[on the evolution of the vitamin b12 content of fermented milk. possibilities of enrichment by the addition of propionibacterium shermannii to the bacterial association]. 196114453983
a vitamin b12 antagonist isolated from propionbacterium shermanii. i. isolation, structure and microbiological examinations. 196114454893
a vitamin b12 antagonist isolated from propionbacterium shermanii. ii. investigations on the origin of factor x. 196114454894
microbiological examination of a natural vitamin b12 antagonist. 196114454895
[synthesis of vitamin b12 by propionic acid bacteria and its relation to the condition of cultivation]. 196214479051
antibiotic resistance of propionibacterium acnes in acne vulgaris.acne vulgaris is a common skin disease affecting all ages. antibiotics remain the most common prescribed agent for the treatment of acne. improper use of antibiotics in the dermatological setting needs to be evaluated to prevent the increasing prevalence of antibiotic resistance.200314515616
influence of histamine in a liver injury model induced by propionibacterium acnes and lipopolysaccharide.in normal mice, plasma histamine levels were 29.4+/-10.1 pmol/ml. when 0.1 microg of lipopolysaccharide (lps) was intravenously injected into propionibacterium acnes (p. acnes)-primed icr mice, histamine levels increased remarkably to 61.2+/-15.9 pmol/ml (p<0.001). an increase was also observed in liver tissues. oral administration of histidine at 200 mg/kg once daily for 5 d before intravenous lps injection increased the plasma alanine aminotransferase (alt) activity to 2936.5+/-356.3 iu/l, a s ...200314519942
ocular infections due to anaerobic bacteria.the increased recovery of anaerobic bacteria in clinical infection has led to greater appreciation of these organisms in ocular infections. in studies that employed adequate method for recovery of anaerobes they were isolated from about a third of patients with conjunctivitis, half of the time in pure culture. the predominant recovered anaerobes were clostridium spp., gram-negative anaerobic bacilli, and peptostreptococcus spp. anaerobic bacteria were also recovered from patients who wore contac ...200114531629
diagnostic management of patients with sapho syndrome: use of mr imaging to guide bone biopsy at ct for microbiological and histological work-up.propionibacterium acnes (p. acnes) is suspected to be involved in the pathophysiology of sapho syndrome, since it has been isolated repeatedly through open surgical bone biopsy. this study demonstrates the role of mri in identifying inflamed bone areas in patients with sapho syndrome and the role of ct-guided bone biopsies in obtaining samples from these areas for microbiological and histopathological investigations, thus obviating open surgery. fourteen consecutive patients with sapho syndrome ...200314534805
first cases of animal diseases published since 2000. 3. cattle.in this third article of a series of papers listing first case reports of animal diseases published since 2000, the following seven cases of cattle diseases are discussed: al amyloidosis. canola oil intoxication. disseminated intracytoplasmic neuronal vacuolation. encephalomyelitis associated with akabane virus infection in adult cows. lower limb deformity: "mirror image duplication of the plantar/palmar half of the distal portion of the digit". lupinus argenteus intoxication. novel propionibact ...200314535583
the in vivo assessment of safety and gastrointestinal survival of an orally administered novel probiotic, propionibacterium jensenii 702, in a male wistar rat model.this study aimed to evaluate in vivo gastrointestinal survival and safety of orally administered probiotic bacterium, propionibacterium jensenii 702, using a male wistar rat model. a high dose of 10(10) cfu/rat/day of p. jensenii 702 was fed to each rat for 81 days. the repeated dose toxicity and translocation of p. jensenii 702 into rat tissues were evaluated, along with the rat faecal beta-glucuronidase activities and dairy propionibacteria counts. results showed that p. jensenii 702 had no ad ...200314563403
fermentation of five sucrose isomers by human dental plaque bacteria.sucrose has five structural isomers: palatinose, trehalulose, turanose, maltulose and leucrose. although these isomers have been reported to be noncariogenic disaccharides, which cannot be utilized by mutans streptococci, there is no information about their fermentability by other bacteria in dental plaque. the purpose of the present study was to examine whether these isomers were fermented by predominant bacteria in human dental plaque. clinical bacterial isolates obtained from dental plaque fr ...200314571118
tylosin resistance in arcanobacterium pyogenes is encoded by an erm x determinant.arcanobacterium pyogenes, a commensal on the mucous membranes of many economically important animal species, is also a pathogen, causing abscesses of the skin, joints, and visceral organs as well as mastitis and abortion. in food animals, a. pyogenes is exposed to antimicrobial agents used for growth promotion, prophylaxis, and therapy, notably tylosin, a macrolide antibiotic used extensively for the prevention of liver abscessation in feedlot cattle in the united states. of 48 a. pyogenes isola ...200314576111
in vitro activities of a new des-fluoro(6) quinolone, garenoxacin, against clinical anaerobic bacteria.the antimicrobial activities of garenoxacin and eight other antibiotics against 641 anaerobic isolates were evaluated with the nccls agar dilution method. overall, the mics of garenoxacin for 50 and 90% of the strains tested (in micrograms per milliliter) were as follows: bacteroides fragilis group, 0.5 and 2; prevotella spp., 0.25 and 2; fusobacterium spp., 0.25 and 0.5; porphyromonas spp., 0.125 and 0.25; bilophila wadsworthia, 0.5 and 1; veillonella spp., 0.25 and 0.5; clostridium spp., 0.25 ...200314576144
delayed postoperative intracerebral abscess caused by proprionibacterium acnes: case report and review of the literature.a case of delayed intracerebral abscess due to proprionibacterium acnes (p. acnes), 18 months after an uneventful excision of a parasagittal haemangiopericytoma in a 61-year-old female is reported. this is highly unusual as cerebral abscess by p. acnes is rare and it occurred so late postoperatively in the absence of known risk factors. we propose the inclusion of cerebral abscess by p. acnes in the differential diagnosis of lesions that mimic tumour recurrences, after clean craniotomies.200314579899
effects of live cultures of lactobacillus acidophilus (strains np45 and np51) and propionibacterium freudenreichii on performance, carcass, and intestinal characteristics, and escherichia coli strain o157 shedding of finishing beef steers.in exp. 1, 240 beef steers (initial bw = 332.8 kg) were used to determine the effects of lactobacillus acidophilus (la) plus propionibacterium freudenreichii (pf) on performance, carcass, and intestinal characteristics; serum iga concentrations; and the prevalence of escherichia coli o157 (ec). cattle were fed a steam-flaked corn-based, 92% concentrate diet, and the four direct-fed microbial (dfm) treatments (12 pens/treatment) included in a randomized complete block design were as follows: 1) c ...200314601871
hydrogel patches containing triclosan for acne treatment.adhesive hydrogel patches containing triclosan (ts) were prepared as an anti-acne dosage form. sodium polyacrylate and carboxymethylcellulose (sodium salt) were used as matrix polymers, and al(3+), produced by the reaction of dihydroxy aluminum aminoacetate and l(+)-tartaric acid, was employed as a crosslinking agent for the negatively charged polymers. the crosslinking reactions were done at 25, 40 and 50 degrees c for predetermined time intervals. the semi-solid gels were obtained only when th ...200314602184
bacteriologic findings in patients with chronic sinusitis.we studied the bacteriology of maxillary sinus aspirates obtained from patients diagnosed with chronic sinusitis. we recovered 659 strains from 510 aspirates; of these, 572 (86.8%) were aerobes and 87 (13.2%) were anaerobes. aerobes only were recovered from 310 of the 510 specimens (60.8%) and anaerobes only from 31 (6.1%). among the 572 aerobic bacteria, the most prevalent organisms were streptococcus viridans (158 strains [27.6%]), streptococcus pneumoniae (67 [11.7%]), corynebacterium species ...200314606178
analysis of clinical isolates of propionibacterium acnes by optimised rapd.random amplification of polymorphic dna (rapd) was evaluated as a genotypic method for typing clinical strains of propionibacterium acnes. rapd can suffer from problems of reproducibility if parameters are not standardised. in this study the reaction conditions were optimised by adjusting template dna concentration and buffer constituents. all isolates were typeable using the optimised rapd protocol which was found to be highly discriminatory (simpson's diversity index, 0.98) and reproducible. t ...200314612236
molecular diagnosis of a vascular prosthesis infection, due to propionibacterium acnes, by amplification and sequencing of 16s rdna.we describe a case of indolent vascular prosthesis infection due to propionibacterium acnes. the microorganism was identified only by amplification and sequencing of 16s rdna, while standard cultures remained negative. this observation underscores the usefulness of molecular techniques for the diagnosis of infection caused by fastidious microorganisms.200314616731
[the influence of a new surface treatment of silicone intracoular lenses with fluoralkylsitan on the adherence of endophthalmitic bacteria in vitro]].dynasilan is a fluoroalkylsilan which is able to bind to surface active molecules of intraocular lenses (iols), thereby offering a new option for surface modification of silicone lenses. the purpose of this in vitro study was to investigate the influence of this new surface treatment on the adherence of two typical endophthalmitis-inducing bacteria ( staphylococcus epidermidis, propionibacterium acnes).200314618356
propionibacterium acnes dna detected in bronchoalveolar lavage cells from patients with sarcoidosis.the causes of sarcoidosis are unknown. propionibacterium acnes is so far the only bacterium to be found in sarcoid lymph nodes. we attempted to detect p. acnes dna in cells recovered by bronchoalveolar lavage (bal) from patients with sarcoidosis.200314620162
diversity of l-leucine catabolism in various microorganisms involved in dairy fermentations, and identification of the rate-controlling step in the formation of the potent flavour component 3-methylbutanal.various microorganisms, belonging to the genera lactococcus, lactobacillus, streptococcus, leuconostoc, bifidobacterium, propionibacterium, brevibacterium, corynebacterium and arthrobacter, used in dairy fermentations such as cheese making, were analysed for their potential to convert leucine into flavour components, most notably 3-methylbutanal. a large variation between and within species was observed for various enzyme activities involved in the conversion pathway, e.g. transaminases, alpha-h ...200414624315
microbiology's principle of biofilms as a major factor in the pathogenesis of acne vulgaris.propionibacterium acnes reside within the pilosebaceous unit in a biofilm. as such, they live in a community of bacteria that encase themselves within an extracellular polysaccharide lining, which the organisms secrete after adherence to the surface. this gylcocalyx polymer acts as a protective exoskeleton and serves as a physical barrier, limiting effective antimicrobial concentrations within the biofilm microenvironment. the gylcocalyx polymer secreted by p. acnes as a biofilm may explain the ...200314636182
effects of opc-6535 on lipopolysaccharide-induced acute liver injury in the rat: involvement of superoxide and tumor necrosis factor-alpha from hepatic macrophages.the objective of this study was to investigate the effects of opc-6535 on propionibacterium acnes-primed and lipopolysaccharide-induced liver injury in the rat.200314639361
is antibiotic resistance in cutaneous propionibacteria clinically relevant? : implications of resistance for acne patients and prescribers.it is well recognized that some patients with acne do not respond adequately to antibiotic therapy. it is important to distinguish antibiotic recalcitrant acne which we would suggest represents acne that shows a diminished response to treatment irrespective of the cause as opposed to 'antibiotic-resistant acne' which is acne that is less responsive to treatment as a direct consequence of skin colonization with resistant propionibacteria. here we show that antibiotic-resistant acne is not just a ...200314640775
is androgenetic alopecia a photoaggravated dermatosis?progressive thinning of the scalp hair in androgenetic alopecia (aga) results in a gradual decline in natural protection of the scalp from ultraviolet radiation (uvr). a number of pathologic conditions of the scalp are evidently related to uvr, particularly photosensitive diseases and disorders of the chronically photodamaged bald scalp. the most important chronic effects of uvr are photocarcinogenesis and solar elastosis. besides these, erosive pustular dermatosis and 'red scalp' are distinct d ...200314657623
assessment of etiologic agents in acne pathogenesis.acne is a chronic inflammatory disease of the pilosebaceous units. traditional etiologic factors include increased sebum production, ductal hyperkeratosis, abnormality of the microbial flora within the pilosebaceous unit, and mediators of inflammation. recent developments do not refute these familial elements, but rather refine particular aspects. interleukin-1a influences hypercornification of the infundibulum as well as the inflammatory response by inducing the production of vascular endotheli ...200314673275
subantimicrobial dose doxycycline for acne and rosacea.acne vulgaris and rosacea present therapeutic challenges due to their chronicity, potential for disfigurement, and psychosocial impact. although pathophysiologically distinct, both conditions have major inflammatory components. consequently, topical and systemic antimicrobial agents are routinely prescribed for extended periods. emergence of resistant strains of propionibacterium acnes, adverse events, and compliance issues associated with chronic systemic tetracycline use have led to new treatm ...200314673277
multiple bacteria in aortic aneurysms.the purpose of the present study was to reexamine the possibility that bacteria, particularly anaerobes, are present in aortic aneurysms.200314681645
endophthalmitis isolates and antibiotic sensitivities: a 6-year review of culture-proven cases.to investigate the spectrum of organisms causing culture-proven endophthalmitis and their sensitivities to commonly used antimicrobial agents.200414700642
comparison of chloroxylenol 0.5% plus salicylic acid 2% cream and benzoyl peroxide 5% gel in the treatment of acne vulgaris: a randomized double-blind study.a 12-week double-blind randomized study was performed to compare benzoyl peroxide 5% (bp) gel and chloroxylenol 0.5% plus salicylic acid 2% (pcmx + sa) cream (nisal cream) for efficacy and adverse reactions. thirty-seven volunteers participated in the study, 19 in the bp group and 18 in the pcmx + sa group. the patients applied the medication twice daily to the entire face. clinical evaluation and lesion counts were obtained at 0, 3, 6, 9 and 12 weeks. at week 12 both groups showed a marked impr ...200314708455
propionibacterium acnes-reactive t helper-1 cells in the skin of patients with acne vulgaris. 200314708633
polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment.in this study, we aimed to investigate the occurrence of several microbial species in cases of failed endodontic therapy by means of the polymerase chain reaction (pcr). study design root canal samples were taken from 22 root-filled teeth with persistent periradicular lesions selected for re-treatment. dna was extracted from the samples and analyzed for the presence of 19 microbial taxa by using the polymerase chain reaction.200414716262
role of core biopsy in diagnosing infection before revision hip arthroplasty.a prerevision core biopsy from a failed hip joint was performed in 41 hips (38 patients) with a high index of suspicion for sepsis to determine its efficacy in diagnosing sepsis. seven hips were known septic failures, and core biopsy was undertaken to confirm resolution of sepsis. forty hips were revised, whereas 1 hip had excision of heterotopic bone. cultures and permanent histologic sections were obtained during subsequent surgeries, and findings were compared with the results of core biopsy. ...200414716655
studies on the cytotoxic effects of propionibacterium acnes strains isolated from cornea.eukaryotic tissue culture appears to be a suitable model for measuring the bacterial cytotoxic effect. propionibacterium acnes strains were isolated from corneal tissue removed by keratoplastic surgery from patients with corneal dystrophy or bullous keratopathy. the cytotoxic effect of the filtrates of 10 p. acnes strains were studied by means of measuring the decrease of the mitochondrial dehydrogenase activities of viable epithelial (hela) and fibroblastic (bhk-21) cell cultures. a time and co ...200414726235
propionibacterium propionicus infection in chronic granulomatous disease. 200414727225
mass spectrometry proteomic analysis of stress adaptation reveals both common and distinct response pathways in propionibacterium freudenreichii.microorganisms used in food technology and probiotics are exposed to technological and digestive stresses, respectively. traditionally used as swiss-type cheese starters, propionibacteria also constitute promising human probiotics. stress tolerance and cross-protection in propionibacterium freudenreichii were thus examined after exposure to heat, acid, or bile salts stresses. adapted cells demonstrated acquired homologous tolerance. cross-protection between bile salts and heat adaptation was dem ...200414730419
[submaxillary adenopathy due to propionibacterium propionicum: atypical presentation]. 200414756998
mobilization of dendritic cell precursors into the circulation by administration of mip-1alpha in mice.dendritic cells (dcs) play a central role in immune responses and may be useful adjuvants for tumor vaccine therapy. we previously reported that f4/80(-)b220(-)cd11c(+) dc precursors expressing the cc chemokine receptors ccr1 and ccr5 are mobilized rapidly into the circulation in mice injected with propionibacterium acnes and are recruited into inflammatory tissue by macrophage inflammatory protein 1alpha (mip-1alpha), which binds to ccr1 and ccr5. here we investigate the mechanisms of dc precur ...200414759987
relationship between periodontal pocket sulfide levels and subgingival species.many species implicated in the pathogenesis of periodontal disease produce volatile sulfur compounds (vsc). this investigation examined the relationship between levels of sulfide and subgingival bacterial species in the same periodontal pockets.200314761124
cutting edge: dendritic cells copulsed with microbial and helminth antigens undergo modified maturation, segregate the antigens to distinct intracellular compartments, and concurrently induce microbe-specific th1 and helminth-specific th2 responses.to examine the ability of dendritic cells (dc) to discriminate between helminth and microbial ag and induce appropriately polarized th responses, mouse dc were copulsed with the helminth ag, schistosome egg ag (sea), along with the bacterium proprionebacterium acnes, pa, and transferred into wild-type mice. strikingly, sea/pa-copulsed dc induced concurrent pa-specific th1 (but not th2) responses and sea-specific th2 (but not th1) responses. although dc exposed to both ag undergo many of the matu ...200414764665
augmentation of tumoricidal activity of human monocytes and macrophages by lymphokines.monocytes were separated from peripheral blood mononuclear cells of normal human donors by adherence on plastic conditioned by cell lines (microexudate-coated plastic) and harvested by exposure to ethylene diamine tetra-acetic acid. cytolytic activity was tested by incubating effector cells for 48 h with the murine sv40-transformed tu5 kidney line or the human lung cancer-derived calu line prelabelled with tritiated thymidine. lymphokine-containing supernatants were obtained from in vitroculture ...198014768696
utilization of coenzyme a by propionibacterium freudenreichii. 195014824067
the mechanism of propionic acid formation by propionibacteria. 195114832422
resolution of persistent periapical infection by endodontic surgery.aim: to examine the surfaces of a root tip removed during surgical endodontic treatment for the presence of microorganisms. summary: the present clinical case illustrates an endodontic retreatment of a maxillary premolar tooth with a fistula and periapical reaction. the case was under treatment for 1 year, during which an intracanal medicament was replaced several times. as the lesion did not decrease and exudate was persistent through the fistula and root canal, root end resection with root end ...200414870760
interconversion of propionate and succinate by propionibacterium pentosaceum. 195114917651
propionibacterium acnes and the pathogenesis of progressive macular hypomelanosis.background: progressive macular hypomelanosis is a common hypopigmentation mainly on the central parts of the trunk, predominantly in young adults, especially women. it is often mistaken for pityriasis versicolor and pityriasis alba. it occurs in all races and has been described in many parts of the world. we discovered follicular red fluorescence restricted to lesional skin. we suspected a relation with a porphyrin-producing bacteria residing in sebum of the pilosebaceous duct, and we therefore ...200414967796
pleurodesis for malignant pleural effusions.approximately half of all patients with metastatic cancer develop a malignant pleural effusion which is likely to lead to a significant reduction in quality of life secondary to symptoms such as dyspnoea and cough. the aim of pleurodesis in these patients is to prevent re-accumulation of the effusion and thereby of symptoms, and avoid the need for repeated hospitalization for thoracocentesis. numerous clinical studies have been performed to try to determine the optimal pleurodesis strategy, and ...200414973997
[treating acne: avoid the development of antibiotic resistance by applying topical and combined therapy]. 200314974325
in vitro assessment of the upper gastrointestinal tolerance of potential probiotic dairy propionibacteria.this study aimed to assess the transit tolerance of potential probiotic dairy propionibacteria strains in human upper gastrointestinal tract in vitro, and to evaluate the effect of food addition on viability of these strains in simulated ph 2.0 gastric juices. the transit tolerance of 13 dairy propionibacteria strains was determined at 37 degrees c by exposing washed cell suspensions to simulated gastric juices at ph values at 2.0, 3.0, and 4.0, and simulated small intestinal juices (ph 8.0) wit ...200414984773
expression and crystallization of several forms of the propionibacterium shermanii transcarboxylase 5s subunit.the dimeric outer 5s subunit of transcarboxylase has been expressed in three different forms and crystallized: native 5s, 5s-his(6) and selenomethione-5s-his(6). all the crystals have an orthorhombic space group, but while native 5s forms primitive orthorhombic crystals, 5s-his(6) crystals are either c-centered or primitive and semet-5s-his(6) crystals are c-centered. crystallization of native 5s requires the addition of lithium sulfate, whereas this salt prevented crystallization of 5s-his(6). ...200414993680
the use of a bacteria detection system to evaluate bacterial contamination in plt concentrates.random-donor plts (rdps) are functional at 7 days. nevertheless, since the mid-1980s, concern for bacterial contamination has caused the storage period to be reduced to 5 days. the ability of a bacteria detection system (bds, pall) to determine bacterial contamination and permit extension of the plt shelf life to 7 days was assessed.200414996189
evaluation of a new generation of plastic culture bottles with an automated microbial detection system for nine common contaminating organisms found in plt components.a microbial detection system (bact/alert 3d, biomérieux [formerly organon teknika]) has previously been validated with a variety of bacterial contaminants in plts. the recovery of nine organisms seeded into plts with new plastic culture bottles was studied in comparison to the current glass bottles. the use of plastic instead of glass would be expected to reduce the risk of injury.200414996192
enhancement of trehalose production in dairy propionibacteria through manipulation of environmental conditions.we have shown that the ability to produce trehalose is widespread within the genus propionibacterium. eighteen strains isolated from dairy sources were screened for trehalose synthesis; the effect of environmental conditions on trehalose production was evaluated in propionibacterium freudenreichii ssp. shermanii nizo b365, a strain that accumulated high amounts of this disaccharide. lactose was the best carbohydrate source for trehalose production, whereas lactate, the substrate that led to the ...200414996463
in situ assessment of porphyrin photosensitizers in propionibacterium acnes.porphyrins are known to be efficient photosensitizer molecules and the combined action of light and porphyrins in propionibacterium acnes have a lethal action on the cells. identification and quantification of in situ porphyrins in p. acnes have been done using an integrating sphere connected to an ordinary absorption spectrophotometer, and the amounts of porphyrins in the cells were quantified by measuring scattering free absorption spectra of the cell suspensions. the concentration of porphyri ...200415018060
propionibacterium acnes lipase in seborrheic dermatitis and other skin diseases and unsei-in.we examined propionibacterium acnes lipase in skin diseases and unsei-in. butyric acid production in axillary seborrheic dermatitis (asd) was higher than in other dermatitis, and that in acne vulgaris (av) was significantly higher than in controls. p. acnes lipase is the pathogenic factor in av and fatty acids produced by lipase might be the pathogenic factor in asd. unsei-in suppressed p. acnes lipase probably because some ingredients have antimicrobial and anti-inflammatory activities.200315018307
two different propionicins produced by propionibacterium thoenii p-127.the bacteriocin gbz-1 was purified from the growth media of propionibacterium thoenii p-127 and was found to have a molecular weight of 6000da. p. thoenii p-127 also known as the producer of the bacteriocin plg-1 (mw 10kda). under specific growth conditions, on semi-solid media, p. thoenii p-127 produced both plg-1 and gbz-1. the n-terminal of gbz-1 was microsequenced, the gene was cloned and the dna sequence was determined and identified. gbz-1 is highly homologous to a protease-activated antim ...200315019204
antibacterial activity of ent-kaurene diterpenoids from rabdosia rosthornii.biological activities of ent-kaurene diterpenoids, rosthornins a-d, isolated from the ether extract of the dried leaves of rabdosia rosthornii (diels) hara (labiatae) were tested. they exhibited antibacterial activity specifically against gram-positive bacteria, among which propionibacterium acnes was noted to be the most susceptible.200415022175
risk factors for mortality in patients with anaerobic bacteremia.risk factors for mortality in anaerobic bacteremia have been incompletely defined. the aims of the present study were to determine clinical significance by pathogen for a broad range of obligate anaerobic organisms isolated from blood, and to define the factors independently associated with mortality among those with clinically significant bacteremia. all patients who had anaerobic bacteria isolated from blood over a 19-month period (from 1 september 1998 to 1 april 2000) at two urban teaching h ...200415024626
enrichment of conjugated linoleic acid in oats (avena sativa l.) by microbial isomerization.a method for microbial isomerization of oat linoleic acid to conjugated linoleic acid (cla) was developed. the method includes hydrolysis of oat lipids in aqueous flour slurries by the endogenous oat lipase. then, the flour slurry containing free linoleic acid is utilized as a substrate for the isomerization reaction carried out by resting cells of propionibacterium freudenreichii ssp. shermanii. the isomerization reaction progressed most effectively when, after the lipid hydrolysis period, the ...200415030240
proinflammatory cytokine production by human keratinocytes stimulated with propionibacterium acnes and p. acnes groel.keratinocytes form the first line of defence in the skin and alert the host to danger by the production of a number of cytokines and chemokines. however, the interaction of commensal microorganisms with keratinocytes has not been well studied.200415030323
anti-stress activity of propionibacterium freudenreichii : identification of a reactivative protein.propionibacterium freudenreichii subsp. shermanii is known to prevent mutations caused by various agents such as n-methyl-n'-nitro-n-nitrosoguanidine, 9-aminoacridine, 4-nitro-quinoline-1-oxide and by uv radiation in both prokaryotic and eukaryotic cells. it was also shown to prevent or repair damage caused by h(2)o(2) or uv radiation in salmonella typhimurium and escherichia coli, a characteristic previously designated as reactivative effect. in order to characterise this effect at the molecula ...200415031664
a thermostable manganese-containing superoxide dismutase from pathogen chlamydia pneumoniae.the gene cp0718 encoding a putative manganese-containing superoxide dismutase of chlamydia pneumoniae ar39 was cloned and expressed in escherichia coli. characterization showed that the expressed protein with a monomeric molecular mass of 23.1 kda had superoxide dismutase (sod) activity and the cofactor of cpsod was a bivalent manganese cation. it is unexpected that this enzyme was hyperthermostable, and maintained about 90% activity after incubation at 70 degrees c for 60 min. manganese binding ...200415043996
a mixed culture of propionibacterium jensenii and lactobacillus paracasei subsp. paracasei inhibits food spoilage yeasts.screening for antimicrobial features of 197 propionibacteria and tests with several antifungal lactobacilli led to the development of three protective cultures containing propionibacterium jensenii sm11 and lactobacillus paracasei subsp. paracasei strain sm20, sm29 or sm63. these cultures showed inhibitory activities (up to 5 orders of magnitude) against yeasts in dairy products such as yoghurt or cheese surface at refrigerator temperatures (6 degrees c) without an influence on the quality prope ...200415046312
adjunct starter properties affect characteristic features of swiss-type cheeses.a large number of microorganisms, both starter microorganisms and non-starter lactic acid bacteria originating from the base milk, or from various contamination sources during cheese manufacture, is associated with cheese ripening and the formation of flavour, texture and aroma. under controlled conditions, emmental and bergkäse, a gruyère-type cheese variety, were produced from pasteurised milk with standard starters and defined strains of facultatively heterofermentative lactobacilli (fhl), an ...200415053357
evaluation of the antibacterial efficacy of diesters of azelaic acid.a number of diesters of the topical dermatosis treatment azelaic (nonanedioic) acid were prepared and tested for antibacterial effect. two esters, bis-[(hexanoyloxy)methyl] nonanedioate and especially bis-[(butanoyloxy)methyl] nonanedioate showed promising activity against acne related bacteria in vitro. no activity of azelaic acid was detected in mueller hinton ii agar at ph 7.3 when using the agar diffusion method, whereas both esters gave zones of growth inhibition. at ph 5.6, activity of aze ...200415066659
prevalence of the genes encoding propionicin t1 and protease-activated antimicrobial peptide and their expression in classical propionibacteria.the purpose of this study was to investigate the frequency of production of the bacteriocin propionicin t1 and the protease-activated antimicrobial peptide (pamp) and their corresponding genes in 64 isolates of classical propionibacteria. this study revealed that these genes are widespread in propionibacterium jensenii and propionibacterium thoenii but absent from the remaining species of classical propionibacteria that were studied. the pro-pamp-encoding gene (pama) was found in 63% of the p. j ...200415066818
[a bacteriology analysis from infected root canals of human deciduous teeth]objective: to provide a basis for therapy and prevention, the flora of infected root canals of human deciduous teeth was analyzed.methods: by immediate-enzyme method, the bacteria compositions of 22 cases with infected root canals of deciduous teeth, including 8 cases with acute periapical inflammation and 14 with chronic ones, were examined. results: among 240 strains bactera, 200 strains were obligate anaerobes, belonging to the genera peptostreptococcus, bacteriodes, veillonella, eubacterrum, ...199815071650
pulmonary granulomas caused experimentally in mice by a recombinant trigger-factor protein of propionibacterium acnes.etiology of sarcoidosis remains unknown. a trigger factor from propionibacterium acnes causes a cellular immune response in some sarcoid patients but not in nonsarcoid subjects. we examined whether experimentally induced hypersensitivity to the trigger factor gives rise to granulomas. female c57bl/6 mice primed intravenously with p. acnes or not were sensitized with recombinant-protein rp35, a fragment of p. acnes trigger factor, and complete freund's adjuvant. in controls, rp35 was replaced wit ...200315074354
topical retinoid and antibiotic combination therapy for acne management.the agents most commonly used in combination for the management of acne include topical retinoids and antibiotics. topical retinoids normalize desquamation of the follicular epithelium, whereas antibiotics inhibit the growth of p. acnes and the production of free fatty acids. this therapeutic combination decreases comedogenesis, bacterial growth, and inflammation, thus targeting three of the four pathogenic factors associated with acne. efficacy and tolerance are maximized with combination thera ...200415098969
purification and characterisation of lipoglycan macroamphiphiles from propionibacterium acnes.lipidated macroamphiphiles such as the lipoteichoic acids and mycobacterial lipoarabinomannans are cell envelope components of gram-positive bacteria that have been extensively associated with the pathogenesis of disease. in order to study such associations, purification of these macroamphiphiles is essential for resolving their structures and diverse biological effects. we describe herein a method for purification of lipoglycan components from propionibacterium acnes. this method uses the exist ...200415103239
topical antibacterial treatments for acne vulgaris : comparative review and guide to selection.topical antibacterial agents are an essential part of the armamentarium for treating acne vulgaris. they are indicated for mild-to-moderate acne, and are a useful alternative for patients who cannot take systemic antibacterials. topical antibacterials such as clindamycin, erythromycin, and tetracycline are bacteriostatic for propionibacterium acnes, and have also been demonstrated to have anti-inflammatory activities through inhibition of lipase production by p. acnes, as well as inhibition of l ...200415109272
characterization of thoeniicin 447, a bacteriocin isolated from propionibacterium thoenii strain 447.fifteen strains of propionibacteria, isolated from dairy products, were screened for the production of bacteriocins. propionibacterium thoenii 447 produced an antimicrobial peptide, thoeniicin 447, which acted bactericidal against lactobacillus delbrueckii subsp. bulgaricus and bacteriostatic against propionibacterium acnes. thoeniicin 447 remained active after 15 min at 100 degrees c and after 30 min of incubation at ph 1-10. the peptide was inactivated when treated with pepsin, pronase, alpha- ...200415109792
shunt nephritis with positive titers for anca specific for proteinase 3.the authors report a case of shunt nephritis with antineutrophil cytoplasmic autoantibody (anca) and review 2 similar cases. a 55-year-old man was admitted to our hospital for continuous fever and foot edema in 2002. a ventriculoperitoneal shunt was implanted because of a brain abscess and subsequent hydrocephalus in 1987; it was changed to a ventriculoatrial (va) shunt in 1995. urinary analysis showed proteinuria (5.4 g/d) and microscopic hematuria. laboratory data showed renal dysfunction and ...200415112193
late-onset central nervous system shunt infections with propionibacterium acnes: diagnosis and management. 200415118785
propionibacterium acnes biotypes and susceptibility to minocycline and keigai-rengyo-to.propionibacterium acnes is the predominant organism in acne lesions, but the sensitivity of different biotypes of p. acnes to therapeutic agents has seldom been reported.200415125499
dietary supplementation with lactobacillus- and propionibacterium-based direct-fed microbials and prevalence of escherichia coli o157 in beef feedlot cattle and on hides at harvest.the objective of this study was to describe the prevalence of escherichia coli o157 in the feces and on the hides of finishing beef cattle fed a standard diet and those fed diets supplemented with direct-fed microbials. two hundred forty steers received one of four treatments throughout the feeding period: (i) control: no added microbials; (ii) hnp51: high dose of lactohacillius acidophilus strain np 51 (10(9) cfu per steer daily) and propionibacterium freudenreichii (10(9) cfu per steer daily); ...200415151223
sensitization of gram-negative and gram-positive bacteria to jenseniin g by sublethal injury.jenseniin g, a bacteriocin produced by propionibacterium thoenii p126, is active against related propionibacteria and some lactic acid bacteria and is sporostatic to botulinal spores. the objective of this study was to evaluate the effects of sublethal stress on jenseniin g activity. bacillus cereus, enterococcus faecalis, escherichia coli, listeria monocytogenes, salmonella typhimurium, shigella flexneri, staphylococcus aureus, and yersinia enterocolitica were subjected to temperature, acid, an ...200415151241
in vitro activities of the new semisynthetic glycopeptide telavancin (td-6424), vancomycin, daptomycin, linezolid, and four comparator agents against anaerobic gram-positive species and corynebacterium spp.telavancin is a new semisynthetic glycopeptide anti-infective with multiple mechanisms of action, including inhibition of bacterial membrane phospholipid synthesis and inhibition of bacterial cell wall synthesis. we determined the in vitro activities of telavancin, vancomycin, daptomycin, linezolid, quinupristin-dalfopristin, imipenem, piperacillin-tazobactam, and ampicillin against 268 clinical isolates of anaerobic gram-positive organisms and 31 corynebacterium strains using agar dilution meth ...200415155214
assessing survival of dairy propionibacteria in gastrointestinal conditions and adherence to intestinal epithelia.the genus propionibacterium consists of two principal groups, cutaneous and classical or dairy. cutaneous species are predominant members of the microbial population of human skin and have also been isolated from the feces of humans and other vertebrate animals. they are often considered opportunistic organisms and have been occasionally associated with infections in humans. dairy propionibacteria are microorganisms extensively used in the industry for manufacture of swiss-type cheeses and biolo ...200415156053
evidence for recruitment of plasmacytoid dendritic cell precursors to inflamed lymph nodes through high endothelial venules.recruitment of dendritic cells (dcs) to lymph nodes (lns) is pivotal to the establishment of immune response. whereas dcs have been proven to undergo afferent lymphatic pathway to enter lns from peripheral tissues, a question remains if dcs also migrate into lns directly from the circulation. here we demonstrate that plasmacytoid dc (pdc) precursors can transmigrate across high endothelial venules (hevs) of inflamed lns in mice. bacterial infection induces a significant number of pdc and myeloid ...200415159375
[bact-alert automatized system for blood cultures: 5 vs 7 days of incubation. first argentine multicentre study].bact-alert automatized system for blood cultures: 5 vs 7 days of incubation. first argentine multicentre study. between january and december 2001, we analyzed 80,141 blood cultures by the bact-alert system (14,960 fan aerobics, 3,855 fan anaerobic, 11,114 standards aerobics, 11,367 standards anaerobic, 12,054 pediatrics and 26,791 fan pediatrics bottles) and 44.235 series from 27.615 patients at eight hospitals of buenos aires city, one of la plata city and three of the buenos aires province. a ...200415174746
pcr detection and 16s rrna sequence-based phylogeny of a novel propionibacterium acidipropionici applicable for enhanced fermentation of high moisture corn.the aims of this study were to develop a sensitive and more rapid detection of propionibacterium acidipropionici dh42 in silage and rumen fluid samples, and to explore its 16s rrna sequence-based phylogeny.200415186440
determination of the binding specificity of the 12s subunit of the transcarboxylase by saturation transfer difference nmr.in this study we present the characterization of the interaction of biotin and methylmalonyl-coa (mmcoa) with the carboxyltransferase subunit (12s) from the transcarboxylase (tc) from propionibacterium shermanii. this biotin dependent multienzyme complex catalyses the transfer of carbon dioxide from methylmalonyl-coa (mmcoa) to pyruvate. the saturation transfer difference nmr (std) technique was performed to determine the binding epitope from biotin and mmcoa to the 12s subunit. we could show by ...200415188046
antimicrobial activities of eucalyptus leaf extracts and flavonoids from eucalyptus maculata.we investigated the antimicrobial activities of eucalyptus leaf extracts to find effective antibacterial agents.200415189289
survey of bacterial proteins released in cheese: a proteomic approach.during the ripening of emmental cheese, the bacterial ecosystem confers its organoleptic characteristics to the evolving curd both by the action of the living cells, and through the release of numerous proteins, including various types of enzymes into the cheese when the cells lyse. in emmental cheese these proteins can be released from thermophilic lactic acid bacteria used as starters like lactobacillus helveticus, lb delbruecki subsp. lactis and streptococcus salivarius subsp. thermophilus an ...200415193805
evidence for diversity within propionibacterium acnes: a comparison of the t-cell stimulatory activity of isolates from inflammatory acne, endocarditis and the laboratory.propionibacterium acnes is primarily associated with the pathogenesis of acne vulgaris but reports are increasing in number implicating p. acnes in other diseases such as abscess formation, meningitis and endocarditis. the pathogenicity of p. acnes is thought to be partly due to the interaction of the bacterium with the immune system. historically, investigations have focused on humoral and cell-mediated immune responses to p. acnes antigens without attention to the possibility that different an ...200415196160
the role of pulsed light and heat energy (lhe) in acne clearance.propionibacterium acnes synthesize and store a large amount of porphyrins. once the porphyrin is exposed to visible light it becomes chemically active and transfers to an excited state, resulting in the formation of singlet oxygen, which combines with cell membranes to destroy the p. acnes. this process is dependent on the rate of production of excited porphyrin molecules, which is influenced by the concentration of porphyrins, the concentration of photons, the temperature, and the wavelength of ...200415203999
actinomycosis: an often forgotten diagnosis.we report a case of actinomycosis presenting as a chest wall mass in a 35 year-old man. thoracic actinomycosis poses a diagnostic challenge to clinicians not only because it is uncommon and often forgotten, but also because culture of the causative microbes is technically difficult. the classic microscopic appearance of this gram-positive bacteria associated with surrounding sulfur granules often forms the basis of diagnosis.200415213087
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