Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| production of indole from l-tryptophan and effects of these compounds on biofilm formation by fusobacterium nucleatum atcc 25586. | the l-tryptophan degradation product indole is a purported extracellular signaling molecule that influences biofilm formation in various bacteria. here we analyzed the mechanisms of indole production in fusobacterium nucleatum and the effects of tryptophan and indole on f. nucleatum planktonic and biofilm cells. the amino acid sequence deduced from the fn1943 gene in f. nucleatum atcc 25586 was 28% identical to that deduced from tnaa in escherichia coli, which encodes tryptophanase catalyzing th ... | 2010 | 20472741 |
| competition between yogurt probiotics and periodontal pathogens in vitro. | to investigate the competition between probiotics in bio-yogurt and periodontal pathogens in vitro. | 2010 | 20491536 |
| identification of immunological parameters associated with the alveolar bone level in periodontal patients. | the present study was performed to clarify the relationship between periodontal disease severity and selected immunological parameters consisting of serum igg titer against periodontopathogenic bacteria, the expression of the helper t-cell cytokine by gingival mononuclear cells, and patients' immunoreactivity to cross-reactive heat shock protein (hsp) epitope peptide from p. gingivalis hsp60. | 2010 | 20498762 |
| in vitro cytokine responses to periodontal pathogens: generalized aggressive periodontitis is associated with increased il-6 response to porphyromonas gingivalis. | generalized aggressive periodontitis (gagp) is an inflammatory condition resulting in destruction of tooth-supporting tissues. we examined the production of il-1beta, il-6, tumour necrosis factor (tnf)-alpha, il-12 and il-10 in cultures of peripheral mononuclear cells (mnc) from 10 patients with gagp and 10 controls stimulated with periodontal pathogens or a control antigen, tetanus toxoid (tt) in the presence of autologous serum. the pathogens used were porphyromonas gingivalis, prevotella inte ... | 2010 | 20500696 |
| the recolonization hypothesis in a full-mouth or multiple-session treatment protocol: a blinded, randomized clinical trial. | to test recolonization of periodontal lesions after full-mouth scaling and root planing (fm-srp) or multiple session-srp (ms-srp) in a randomized clinical trial and whether fm-srp and ms-srp result in different clinical outcomes. | 2010 | 20507375 |
| mechanical non-surgical treatment of peri-implantitis: a single-blinded randomized longitudinal clinical study. ii. microbiological results. | peri-implantitis is common in patients with dental implants. we performed a single-blinded longitudinal randomized study to assess the effects of mechanical debridement on the peri-implant microbiota in peri-implantitis lesions. | 2010 | 20507380 |
| [reversible metronidazole-induced encephalopathy and hypertrophic olivary degeneration]. | 2010 | 20508563 | |
| effects of a composition containing lactoferrin and lactoperoxidase on oral malodor and salivary bacteria: a randomized, double-blind, crossover, placebo-controlled clinical trial. | we report a clinical trial of the effects of test tablets containing bovine lactoferrin and lactoperoxidase on oral malodor and salivary bacteria. fifteen subjects with volatile sulfur compounds (vscs) in mouth air above the olfactory threshold (h(2)s >1.5 or ch(3)sh >0.5 ng/10 ml) as detected by gas chromatography were enrolled in the trial. either a test or a placebo tablet was ingested twice at 1-h intervals in two crossover phases. mouth air was monitored for vsc levels at the baseline befor ... | 2010 | 20512389 |
| antimicrobial efficacy of 3 oral antiseptics containing octenidine, polyhexamethylene biguanide, or citroxx: can chlorhexidine be replaced? | use of oral antiseptics decreases the bacterial load in the oral cavity. | 2010 | 20518635 |
| gram-negative periodontal bacteria induce the activation of toll-like receptors 2 and 4, and cytokine production in human periodontal ligament cells. | periodontitis is a bacterially induced chronic inflammatory disease. toll-like receptors (tlrs), which could recognize microbial pathogens, are important components in the innate and adaptive immune systems. both qualitatively and quantitatively distinct immune responses might result from different bacteria stimulation and the triggering of different tlrs. this study explores the interaction of porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, and aggregatibacter actinomy ... | 2010 | 20528699 |
| folding of a transcriptionally acting preq1 riboswitch. | 7-aminomethyl-7-deazaguanine (preq(1)) sensitive mrna domains belong to the smallest riboswitches known to date. although recent efforts have revealed the three-dimensional architecture of the ligand-aptamer complex less is known about the molecular details of the ligand-induced response mechanism that modulates gene expression. we present an in vitro investigation on the ligand-induced folding process of the preq(1) responsive rna element from fusobacterium nucleatum using biophysical methods, ... | 2010 | 20534493 |
| antimicrobial activity of n-6, n-7 and n-9 fatty acids and their esters for oral microorganisms. | this study is to assess the antibacterial activity of omega-6, -7, -9 (n-6, n-7, n-9) fatty acids against various oral microorganisms. | 2010 | 20541177 |
| antibiotic resistance of subgingival species in chronic periodontitis patients. | the increasing rate of resistance of microorganisms to penicillin and other antibiotics has generated concern among health authorities in latin america. the present investigation determined the in vitro susceptibility of porphyromonas gingivalis, fusobacterium nucleatum, black-pigmented prevotella spp. and aggregatibacter actinomycetemcomitans to metronidazole, amoxicillin, amoxicillin/clavulanic acid, clindamycin and moxifloxacin in patients with chronic periodontitis. | 2010 | 20546113 |
| [usefulness and limit of gram staining smear examination]. | gram staining is one of the most simple and inexpensive methods for the rapid diagnosis of bacterial and fungal infections. it yields results much faster than culture, and provides important data for the patient's treatment and prognosis. however, a difference exists in the quality and quantity of information yielded by gram staining smears based on the experience and knowledge of those conducting the tests. therefore, a risk of misdiagnosis based on the information obtained from gram staining s ... | 2010 | 20560458 |
| hiv-1 reactivation induced by the periodontal pathogens fusobacterium nucleatum and porphyromonas gingivalis involves toll-like receptor 2 [corrected] and 9 activation in monocytes/macrophages. | although oral coinfections (e.g., periodontal disease) are highly prevalent in human immunodeficiency virus type 1-positive (hiv-1(+)) patients and appear to positively correlate with viral load levels, the potential for oral bacteria to induce hiv-1 reactivation in latently infected cells has received little attention. we showed that hiv-1 long terminal repeat (ltr) promoter activation can be induced by periodontopathogens in monocytes/macrophages; nevertheless, the mechanisms involved in this ... | 2010 | 20610663 |
| differential and coordinated expression of defensins and cytokines by gingival epithelial cells and dendritic cells in response to oral bacteria. | epithelial cells and dendritic cells (dcs) both initiate and contribute to innate immune responses to bacteria. however, much less is known about the coordinated regulation of innate immune responses between gecs and immune cells, particularly dcs in the oral cavity. the present study was conducted to investigate whether their responses are coordinated and are bacteria-specific in the oral cavity. | 2010 | 20618959 |
| new aspects and new concepts of maintaining "microbiological" health. | the human mouth is host to a diverse collection of microorganisms including bacteria, viruses, fungi and protozoa. recent advances using molecular methods for the analysis of complex bacterial communities have demonstrated the richness of the oral bacterial biota and the presence of numerous previously undescribed lineages. dental plaque forms in a structured way with pioneer species able to colonise pellicle-coated enamel followed by secondary plaque formers such as fusobacterium nucleatum that ... | 2010 | 20621241 |
| antiaggregation potential of berry fractions against pairs of streptococcus mutans with fusobacterium nucleatum or actinomyces naeslundii. | coaggregation is an interspecies adhesion process, which is essential to the development of dental plaque. this is an in vitro study of the composition of the soluble solids in the berry juice molecular size fractions (<10 kda, fi; 10-100 kda, fii; >100 kda, fiii) derived from apple, bilberry, blackcurrant, cloudberry, crowberry and lingonberry and their ability to inhibit and reverse coaggregation of the pairs of common species in dental plaque: streptococcus mutans with fusobacterium nucleatum ... | 2011 | 20623601 |
| examination of periodontal pathogens in stenotic valve specimens and in whole blood samples in patients affected by aortic valve stenosis and chronic periodontitis. | periodontitis may be a risk factor for atherosclerosis and coronary heart disease. the influence of periodontal pathogens in cardiovascular diseases needs further investigation. therefore, the aims of this clinical study are: to test the presence of periodontal bacteria dna in aortic valves and to assess the concomitant presence of the same periodontal bacteria dna in whole blood samples in patients affected by aortic valve stenosis and chronic periodontitis. nineteen consecutive patients (12 ma ... | 2010 | 20646351 |
| microbiota around teeth and dental implants in periodontally healthy, partially edentulous patients: is pre-implant microbiological testing relevant? | this study aimed to assess the prevalence of seven periodontal marker pathogens, before implant placement and 1 yr after loading, in periodontally healthy individuals and to assess the long-term effectiveness of pre-implant reduction of pathogens to below threshold levels. in 93 individuals needing single tooth replacement, pooled subgingival microbiological samples from standard sites were cultured and analyzed before implant treatment and 1 yr after loading. threshold levels commonly used in p ... | 2010 | 20662908 |
| fusobacterium nucleatum regulation of neutrophil transcription. | abnormal neutrophil responses have been observed in periodontitis patients, including hyper-reactivity in terms of production of reactive oxygen species (ros) following exposure to the key quorum-sensing plaque bacterium, fusobacterium nucleatum. this study was designed to characterize the transcriptional response of neutrophils to f. nucleatum. | 2011 | 20663022 |
| energy dose parameters affect antimicrobial photodynamic therapy-mediated eradication of periopathogenic biofilm and planktonic cultures. | this study evaluated the in vitro efficacy of a commercially available apdt system in eradication of the periopathogens porphyromonas gingivalis, fusobacterium nucleatum, and aggregatibacter actinomycetemcomitans in both planktonic and biofilm cultures. | 2010 | 20666581 |
| vaccination of mice with porphyromonas gingivalis or fusobacterium nucleatum modulates the inflammatory response, but fails to prevent experimental periodontitis. | to assess the potential of using vaccination with porphyromonas gingivalis or fusobacterium nucleatum, in modulating local subcutaneous inflammatory response and alveolar bone loss following coinfection with both bacteria. | 2010 | 20670341 |
| a brain abscess following dental extractions in a patient with hereditary hemorrhagic telangiectasia. | oral and maxillofacial surgeons must be aware of the potentially life-threatening complications of dental extractions in patients with hereditary haemorrhagic telangiectasia because of their high prevalence of pulmonary arteriovenous fistulas. despite the lack of evidence-based guidelines, antibiotic cover should be given to patients with hht who require oral surgery according to the same rules as those used for patients at high risk of bacterial endocarditis. | 2010 | 20719417 |
| polybacterial challenge effects on cytokine/chemokine production by macrophages and dendritic cells. | to investigate the polymicrobial infection of periodontal disease, which elicits inflammatory mediators/cytokines/chemokines in the local gingival tissues, and a polybacterial challenge of antigen-presenting cells, e.g. macrophages and dendritic cells (dcs), at the mucosal surface. | 2011 | 20798974 |
| fusobacterium nucleatum outer membrane proteins fap2 and radd induce cell death in human lymphocytes. | bacterially induced cell death in human lymphocytes is an important virulence factor for pathogenic bacteria. previously discovered mechanisms of bacterially induced cell death are predominantly based on the transfer of bacterial proteins to the target host cell, such as the toxins secreted through type i, ii, and vi secretion systems or effector proteins injected through type iii, iv, and vb secretion systems. here, we report a mechanism employed by the gram-negative oral pathogen fusobacterium ... | 2010 | 20823215 |
| a 6-month study of the effects of 0.3% triclosan/copolymer dentifrice on dental implants. | supportive therapy to maintain dental implants is increasingly important. this study examined the effect of a 0.3% triclosan/2% copolymer dentifrice on oral biofilms and gingival inflammation (gi) on dental implants and peri-implant tissues. | 2011 | 20831669 |
| fusobacterium nucleatum binding to complement regulatory protein cd46 modulates the expression and secretion of cytokines and matrix metalloproteinases by oral epithelial cells. | periodontitis is a chronic inflammatory disease that results in the destruction of the supporting tissues of the teeth. gingival epithelial cells are an important mechanical barrier and participate in the host inflammatory response to periodontopathogens. the aim of the present study is to investigate the capacity of fusobacterium nucleatum to bind to the complement regulatory protein cd46 expressed by oral epithelial cells and to determine the impact of the binding on the gene expression and pr ... | 2011 | 20843232 |
| fusobacterium nucleatum-associated beta-defensin inducer (fad-i): identification, isolation, and functional evaluation. | human β-defensins (hbds) are small, cationic antimicrobial peptides, secreted by mucosal epithelial cells that regulate adaptive immune functions. we previously reported that fusobacterium nucleatum, a ubiquitous gram-negative bacterium of the human oral cavity, induces human β-defensin 2 (hbd2) upon contact with primary oral epithelial cells. we now report the isolation and characterization of an f. nucleatum (atcc 25586)-associated defensin inducer (fad-i). biochemical approaches revealed a ce ... | 2010 | 20847052 |
| antimicrobial activity of sodium citrate against streptococcus pneumoniae and several oral bacteria. | the aim of this study is to assess the antibacterial activity of sodium citrate against streptococcus pneumoniae and several oral bacteria. | 2010 | 20849395 |
| apical root canal microbiota as determined by reverse-capture checkerboard analysis of cryogenically ground root samples from teeth with apical periodontitis. | bacteria located in the apical root canal system potentially participate in the pathogenesis of apical periodontitis. detection and identification of apical bacteria can be compromised because of limitations in conventional sampling and identification procedures. this study identified several bacterial taxa in the apical and middle/coronal segments of primarily infected root canal system by using pulverized root segments and a culture-independent molecular method. | 2010 | 20850664 |
| isolation of a novel bacteriophage specific for the periodontal pathogen fusobacterium nucleatum. | fusobacterium nucleatum is a periodontal pathogen that has been directly associated with the development and progression of periodontal disease, a widespread pathology that affects the support tissues of the tooth. we isolated a new bacteriophage (fnpφ02) that specifically infects this bacterium. transmission electron microscopy showed that the virion is composed of an icosahedral head and a segmented tail. the size of the phage genome was estimated to be approximately 59 kbp of double-stranded ... | 2010 | 20851973 |
| [effect of compound chinese traditional medicine on infected root canal bacteria biofilm]. | to assess the efficacy of compound chinese traditional medicine(ctm), which composed of gallic acid, magnolol and polysaccharide of blettila striata, against the infected root canal bacterial biofilm. | 2010 | 20871960 |
| effect of biofilm on the repair bond strengths of composites. | composite restorations degrade during wear, but it is unknown how wear affects the composite surface and influences composite-to-composite bonding in minimally invasive repair. here, it is hypothesized that in vitro exposure of composites to oral biofilm yields clinically relevant degradation of composite surfaces, and its influence on composite-to-composite bonding is determined. biofilms on composite surfaces in vitro increased their roughness and decreased filler particle exposure, except for ... | 2010 | 20940354 |
| bactericidal activity of stabilized chlorine dioxide as an endodontic irrigant in a polymicrobial biofilm tooth model system. | the purpose of this study was to determine bactericidal efficacy of 0.04% stabilized chlorine dioxide, 3% sodium hypochlorite, 2% chlorhexidine gluconate, and sterile distilled water in a polymicrobial biofilm model. | 2010 | 20951304 |
| oxygen tension modulates the cytokine response of oral epithelium to periodontal bacteria. | there is an inverse relationship between pocket depth and pocket oxygen tension with deep pockets being associated with anaerobic bacteria. however, little is known about how the host tissues respond to bacteria under differing oxygen tensions within the periodontal pocket. | 2010 | 20955352 |
| murine fetoplacental infection models. | 2010 | 20956576 | |
| setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens. | caries and periodontitis are important human diseases associated with formation of multi-species biofilms. the involved bacteria are intensively studied to understand the molecular basis of the interactions in such biofilms. this study established a basic in vitro single and mixed-species culture model for oral bacteria combining three complimentary methods. the setup allows a rapid screening for effects in the mutual species interaction. furthermore, it is easy to handle, inexpensive, and repro ... | 2010 | 20957048 |
| photoinactivation of f. nucleatum and p. gingivalis using the ruthenium-based rd3 sensitizer and a conventional halogen lamp. | the photodynamic therapy (pdt) is an alternative method to suppress oral pathogens by the activation of a photosensitizer with laser light. the aim of this study was to investigate the phototoxic effect of three ruthenium-based photosensitizers on fusobacterium nucleatum and porphyromonas gingivalis. | 2010 | 21036348 |
| early bacterial colonization and soft tissue health around zirconia and titanium abutments: an in vivo study in man. | aim: to compare the early bacterial colonization and soft tissue health of mucosa adjacent to zirconia (zro(2) ) and titanium (ti) abutment surfaces in vivo. materials and methods: twenty edentulous subjects received two endosseous mandibular implants. the implants were fitted with either a zro(2) or a ti abutment (non-submerged implant placement, within-subject comparison, left-right randomization). sulcular bacterial sampling and the assessment of probing pocket depth, recession and bleeding o ... | 2010 | 21054554 |
| intracellular degradation of fusobacterium nucleatum in human gingival epithelial cells. | the role of fusobacterium nucleatum in oral health and disease is controversial. we have previously shown that f. nucleatum invades gingival epithelial cells. however, the destiny of the internalized f. nucleatum is not clear. in the present study, the intracellular destiny of f. nucleatum and its cytopathic effect on gingival epithelial cells were studied. the ability of f. nucleatum and seven other oral bacterial species to invade immortalized human gingival epithelial (hok-16b) cells were com ... | 2010 | 21057979 |
| systemic inflammatory responses in progressing periodontitis during pregnancy in a baboon model. | this study tested the hypothesis that pregnant female baboons exhibit increased levels of various inflammatory mediators in serum resulting from ligature-induced periodontitis, and that these profiles would relate to periodontal disease severity/extent in the animals. the animals were sampled at baseline (b), mid-pregnancy (mp; two quadrants ligated) and at delivery (d; four quadrants ligated). all baboons developed increased plaque, gingival inflammation and bleeding, pocket depths and attachme ... | 2010 | 21070210 |
| denaturing gradient gel electrophoresis analysis with different primers of subgingival bacterial communities under mechanical debridement. | dgge of 16s rdna is one of the most frequently used methods to study microbial communities. in this study, the dgge profiles of different 16s rdna regions of the periodontal pathogens porphyromonas gingivalis, fusobacterium nucleatum, and prevotella nigrescens were investigated. the results suggested that v3-v5 and v6-v8 fragments may be suitable for community analysis of subgingival bacteria. further analysis of subgingival samples with v3-v5 and v6-v8 regions as target fragments suggested that ... | 2010 | 21155359 |
| antibacterial and anti-inflammatory activities of 4-hydroxycordoin: potential therapeutic benefits. | 4-hydroxycordoin (1), a natural isopentenyloxychalcone, is a plant secondary metabolite that is relatively rare. since there are very few reports about the biological activities of 1, its potential benefits for periodontal disease were investigated. a marked and dose-dependent antibacterial activity of 1 was observed against the three major periodontal pathogens, porphyromonas gingivalis, fusobacterium nucleatum, and prevotella intermedia. moreover, compound 1 showed an antiadhesion effect, sinc ... | 2010 | 21158427 |
| microbiota of dentinal caries as assessed by reverse-capture checkerboard analysis. | this study aimed to identify the microbiota of different layers of dentinal caries by using a culture-independent molecular biology approach. | 2010 | 21160186 |
| comparison of the use of the halimeter and the oral chroma™ in the assessment of the ability of common cultivable oral anaerobic bacteria to produce malodorous volatile sulfur compounds from cysteine and methionine. | to compare the use of the halimeter and the oral chroma™ to assess the ability of common oral anaerobic bacteria isolated from the kuwaiti population to produce volatile sulfur compounds (vscs). | 2010 | 21160219 |
| [detection of putative periodontal pathogens of periodontitis with type 2 diabetes]. | to detect the putative periodontal pathogens in the subgingival plaque of chronic periodontitis (cp) patients with type 2 diabetes mellitus (dm) and to discuss the role of the periodontal pathogens in the cp and type 2 dm patients. | 2010 | 21163008 |
| effect of smoking on subgingival microflora of patients with periodontitis in japan. | smoking is a risk factor for periodontitis. to clarify the contribution of smoking to periodontitis, it is essential to assess the relationship between smoking and the subgingival microflora. the aim of this study was to gain an insight into the influence of smoking on the microflora of japanese patients with periodontitis. | 2011 | 21208407 |
| identification of periodontal pathogens and severity of periodontitis in patients with and without chronic kidney disease. | in this study of patients with chronic periodontitis (cp), the severity of the disease and the main periodontal pathogens identified in patients with chronic kidney disease (ckd) were compared with those detected in individuals without systemic disease. | 2011 | 21211789 |
| effect of nicotine, cotinine and cigarette smoke extract on the neutrophil respiratory burst. | to determine the effect of nicotine, cotinine and cigarette smoke extract (cse) on the neutrophil respiratory burst and their effect on activation of the nuclear factor-κb (nfκb) pathway in oral epithelium. | 2011 | 21214612 |
| predation of oral pathogens by bdellovibrio bacteriovorus 109j. | periodontal diseases are multifactorial infections elicited by a complex of primarily gram-negative bacteria that interact with host tissues and lead to the destruction of the periodontal structures. bdellovibrio bacteriovorus is a gram-negative bacterium that preys upon other gram-negative bacteria. it was previously shown that b. bacteriovorus has an ability to attack and remove surface-attached bacteria or biofilms. in this study, we examined the host specificity of b. bacteriovorus strain 10 ... | 2010 | 21214870 |
| killing of anaerobic pathogens by predatory bacteria. | recently, the predation of bdellovibrio bacteriovorus on a periodontal pathogen has been described. the current study explores the potential antimicrobial activity of a range of predatory bacteria against key periodontal pathogens. a number of representatives from the bdellovibrio, bacteriovorax and peredibacter lineages (called 'balos') were tested for their activity towards a group of key periodontal pathogens and an optimal multiplicity of infection was established. as the oral cavity contain ... | 2010 | 21214872 |
| fusobacterium nucleatum in periodontal health and disease. | the pathogenesis of periodontitis involves the interplay of microbiota present in the subgingival plaque and the host responses. inflammation and destruction of periodontal tissues are considered to result from the response of a susceptible host to a microbial biofilm containing gram-negative pathogens. antimicrobial peptides are important contributors to maintaining the balance between health and disease in this complex environment. these include several salivary antimicrobial peptides such as ... | 2011 | 21220789 |
| antimicrobial activity of diterpenes from viguiera arenaria against endodontic bacteria. | six pimarane-type diterpenes isolated from viguiera arenaria baker and two semi-synthetic derivatives were evaluated in vitro against a panel of representative microorganisms responsible for dental root canal infections. the microdilution method was used for the determination of the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) against porphyromonas gingivalis, prevotella nigrescens, prevotella intermedia, prevotella buccae, fusobacterium nucleatum, bacteroi ... | 2011 | 21233793 |
| invasive potential of gut mucosa-derived fusobacterium nucleatum positively correlates with ibd status of the host. | background:: fusobacterium nucleatum is a heterogeneous oral pathogen that is also a common resident of the human gut mucosa. given that some strains of f. nucleatum are known to be invasive and proinflammatory in the oral mucosa, we compared strains isolated from patients with inflammatory bowel disease (ibd) with strains isolated from healthy controls to determine 1) whether this species was more commonly associated with ibd patients; and 2) whether gut-derived f. nucleatum strains from ibd pa ... | 2011 | 21234912 |
| effects of toluidine blue-mediated photodynamic therapy on periopathogens and periodontal biofilm: in vitro evaluation. | photodynamic therapy (pdt) is a selective modality of killing targeted cells, mostly known for its application in neoplasms. pdt can be considered to be an alternative method for the elimination of periodontal bacteria from the pocket without harms for the resident tissues. therefore, pdt may replace systemic antibiotics and enhance the effect of mechanical treatments of periodontal defects. this effort focused on the in vitro sensitization of periopathogens (aggregatibacter actinomycetemcomitan ... | 2010 | 21244761 |
| epigenetic regulation of human β-defensin 2 and cc chemokine ligand 20 expression in gingival epithelial cells in response to oral bacteria. | gingival epithelia utilize multiple signaling pathways to regulate innate immune responses to various oral bacteria, but little is understood about how these bacteria alter epithelial epigenetic status. in this study we report that dna methyltransferase (dnmt1) and histone deacetylase expression were decreased in gingival epithelial cells treated with oral pathogen porphyromonas gingivalis and nonpathogen fusobacterium nucleatum. pretreatment with trichostatin a and sodium butyrate, which increa ... | 2011 | 21248725 |
| [fusobacterium nucleatum isolated from a patient presenting lachrymal canaliculitis]. | a 68-year-old woman presented with a painless inflammation of the right superior eyelid that had started several weeks before. the clinical diagnosis concluded in canaliculitis and the solid concretions were surgically extracted from the superior canalicula. the anaerobic bacteria fusobacterium nucleatum sp. nucleatum was isolated. signs dramatically regressed two weeks after surgery followed by one course of oral amoxicillin and clavulanic acid associated with topical tobramycin. the clinical s ... | 2011 | 21255867 |
| [a case of liver abscess caused by fusobacterium nucleatum in a patient with recurrent periodontal diseases]. | fusobacteria are anaerobic gram-negative, non-spore forming bacilli found in normal flora of the oral cavity, urogenital tract, and gastrointestinal tract. fusobacterium nucleatum has been seldom reported as a cause of liver abscess, particularly in immunocompetent hosts. a 55-year-old man with frequent periodontal disease visited our hospital with intermittent fever and headache for 2 months. abdominal ct scan revealed an 8.2 × 6 cm mass in the right hepatic lobe with central low density. absce ... | 2011 | 21258201 |
| salivary infectious agents and periodontal disease status. | the potential of salivary microorganisms to diagnose periodontal disease and to guide periodontal treatment is a research topic of current interest. this study aimed to determine whether the salivary counts of periodontopathic microbes correlated with the periodontal pocket counts of the same infectious agents, and whether the salivary counts of the test infectious agents could distinguish among individuals with periodontal health and various types of periodontal disease. | 2011 | 21261620 |
| structure, viability and bacterial kinetics of an in vitro biofilm model using six bacteria from the subgingival microbiota. | there are few in vitro models available in the scientific literature for study of the structure, formation and development of the subgingival biofilm. the purpose of this study was to develop and validate an in vitro biofilm model, using representative selected bacteria from the subgingival microbiota. | 2011 | 21261622 |
| evaluation of the antimicrobial activity of dentifrices on human oral bacteria. | in vitro testing of antimicrobial agents is an important tool in the testing hierarchy, and may provide interesting insights into their potential clinical efficacy. agents with demonstrable in vitro antimicrobial activity may be effective against the same microorganisms in vivo, whereas agents without demonstrable in vitro antimicrobial activity are unlikely to exhibit in vivo antimicrobial activity. in addition, these methods may also be useful in screening antimicrobial agents in product formu ... | 2010 | 21269037 |
| evaluation of three swab transport systems for the maintenance of clinically important bacteria in simulated mono- and polymicrobial samples. | in this study, three swab transport systems were evaluated: m40 transystem, amies broth with a relatively new type of swab (both copan diagnostics, corona, ca, usa), and ssi transportmedium (statens serum institut, copenhagen denmark). the clsi m40-a standard procedures and 11 culture collection strains were used. the transport systems were tested at room temperature for holding times of 0, 24, and 48 h, and both mono- and polymicrobial samples were included. after 24 h of simulated transportati ... | 2011 | 21284737 |
| effect of ozone on periodontopathogenic species-an in vitro study. | the in vitro study was aimed to determine the effect of ozone on periodontopathogenic microorganisms. ozone was generated for 6 s-2?×?24 s (corresponding to 0.56 mg-2?×?2.24 mg of ozone) against 23 mainly anaerobic periodontopathogenic species. agar diffusion test was used as a screening method. then, the killing activity was tested in a serum-free environment and with 25% v/v inactivated serum. further, the effect of ozone on bactericidal activity of native serum was analyzed against fusobacter ... | 2011 | 21287208 |
| assessing the efficacy of vesicle fusion with planar membrane arrays using a mitochondrial porin as reporter. | reconstitution of functionally active membrane protein into artificially made lipid bilayers is a challenge that must be overcome to create a membrane-based biomimetic sensor and separation device. in this study we address the efficacy of proteoliposome fusion with planar membrane arrays. we establish a protein incorporation efficacy assay using the major non-specific porin of fusobacterium nucleatum (foma) as reporter. we use electrical conductance measurements and fluorescence microscopy to ch ... | 2011 | 21295545 |
| the effect of xylitol on the composition of the oral flora: a pilot study. | our aim was to investigate the effect of short-term xylitol consumption on the microbial composition of plaque and saliva. | 2011 | 21311610 |
| the origin of fusobacterium nucleatum involved in intra-amniotic infection and preterm birth. | objective. to evaluate the potential oral origin of fusobacterium nucleatum found in amniotic fluid of women at high risk of preterm birth. methods. a transversal study nested into a cohort study of women with preterm labor and/or preterm premature rupture of membranes was undergone. women with the presence of f. nucleatum in the amniotic fluid and their respective partners were invited to be examined for their periodontal health after delivery, and samples of saliva and subgingival plaque were ... | 2011 | 21314291 |
| the role of acidification in the inhibition of neisseria gonorrhoeae by vaginal lactobacilli during anaerobic growth. | vaginal lactobacilli protect the female genital tract by producing lactic acid, bacteriocins, hydrogen peroxide or a local immune response. in bacterial vaginosis, normal lactobacilli are replaced by an anaerobic flora and this may increase susceptibility to neisseria gonorrhoeae, a facultative anaerobe. bacterial interference between vaginal lactobacilli and n. gonorrhoeae has not been studied in liquid medium under anaerobic conditions. by co-cultivating n. gonorrhoeae in the presence of lacto ... | 2011 | 21329492 |
| short- and medium-chain fatty acids exhibit antimicrobial activity for oral microorganisms. | this study assessed the antibacterial activity of short-, medium-, and long-chain fatty acids against various oral microorganisms. | 2011 | 21333271 |
| testing meningococcal vaccines for mitogenicity and superantigenicity. | proteins with intrinsic mitogenic properties are widely represented in prokaryotes, such as in different streptococcus species (1-3), candida albicans (4), and eikenella corrodens (5). specifically, several bacterial porins of escherichia coli, shigella dysenteriae, salmonella typhimurium, fusobacterium nucleatum, and pathogenic neisseria species have been shown to induce nonspecific proliferation of lymphocytes (6-12). | 2001 | 21336757 |
| salivary blood group antigens and microbial flora. | 2010 | 21356009 | |
| association between periodontal pathogens and risk of nonfatal myocardial infarction. | the direct effect of periodontal pathogens on atherosclerotic plaque development has been suggested as a potential mechanism for the observed association between periodontal disease and coronary heart disease, but few studies have tested this theory. | 2011 | 21375559 |
| rna-oligonucleotide quantification technique (roqt) for the enumeration of uncultivated bacterial species in subgingival biofilms. | approximately 35% of the species present in subgingival biofilms are as yet uncultivated, so their role in periodontal pathogenesis is unknown. the aim of the present study was to develop a high throughput method to quantify a wide range of cultivated and uncultivated taxa in subgingival biofilm samples associated with periodontal disease or health. oligonucleotides targeting the 16s ribosomal dna gene were designed, synthesized and labeled with digoxigenin. these probes were hybridized with the ... | 2011 | 21375703 |
| streptococcus cristatus attenuates fusobacterium nucleatum-induced cytokine expression by influencing pathways converging on nuclear factor-?b. | we previously reported that streptococcus cristatus, an oral commensal, was able to downregulate the interleukin-8 (il-8) response to fusobacterium nucleatum, a putative oral pathogen in oral epithelial cells. the aim of this study was to extend the understanding of how s. cristatus regulates cytokine expression in oral epithelial cells on a broad basis, and investigate whether the modulation of a toll-like receptor (tlr) pathway was involved in this process. kb and tert-2 cells were co-cultured ... | 2011 | 21375705 |
| in-vivo-induced antigenic determinants of fusobacterium nucleatum subsp. nucleatum. | fusobacterium nucleatum plays a pivotal role in dental plaque biofilm formation and is known to be involved in chronic inflammatory systemic disease. however, limited knowledge of f. nucleatum genes expressed in vivo interferes with our understanding of pathogenesis. in this study, we identified f. nucleatum genes induced in vivo using in-vivo-induced antigen technology (iviat). among 30,000 recombinant clones screened, 87 reacted reproducibly with pooled sera from 10 patients with periodontitis ... | 2011 | 21375706 |
| myrsinoic acid b inhibits the production of hydrogen sulfide by periodontal pathogens in vitro. | recently, we reported that myrsinoic acid b purified from myrsine seguinii inhibited methyl mercaptan (ch(3)sh) production by fusobacterium nucleatum jcm8532. since hydrogen sulfide (h(2)s) is the main component of physiological halitosis, while ch(3)sh is involved in pathological oral halitosis, the objective of this study is to determine whether myrsinoic acid b inhibits h(2)s production by oral microorganisms. f. nucleatum, porphyromonas gingivalis and treponema denticola were incubated with ... | 2010 | 21383473 |
| tongue biofilm areal density and tongue coating index. | two methods have been employed to measure the amount of tongue biofilm in humans: (1) areal density of microbes (cfu cm(-2)) (tongue scrape from a known/measured area), or (2) a tongue-coating index, where a human judge assesses the amount of 'coating' by visual inspection. the two methods were compared, both on human volunteers and on artificial biofilms. fusobacterium nucleatum (fn), prevotella intermedia (pi), streptococcus mutans (sm) and peptostreptococcus micros (pm) were obtained from sto ... | 2008 | 21386152 |
| generation of indole/skatole during malodor formation in the salivary sediment model system and initial examination of the oral bacteria involved. | gram negative anaerobic microbial degradation of proteins, peptides and amino acids in saliva leads to production of oral malodor. volatile sulfur compounds (vscs) from cysteine and indole/skatole (i/s) from tryptophan are two major components of breath odor. in this study, salivary mixed bacteria and oral pure cultures were compared for their ability to produce odor from cysteine, tryptophan and salivary supernatant. the vsc malodor inhibitor, zinc, was used to help identify the malodor process ... | 2008 | 21386161 |
| clinical spectrum of bacteraemic fusobacterium infections: from septic shock to nosocomial bacteraemia. | fusobacterium species are anaerobic bacteria that relatively rarely cause sepsis with a variable clinical presentation. | 2011 | 21391770 |
| modeling of diffusion transport through oral biofilms with the inverse problem method. | the purpose of this study was to develop a mathematical model to quantitatively describe the passive transport of macromolecules within dental biofilms. | 2010 | 21404968 |
| clinical periodontal and microbiologic parameters in patients with rheumatoid arthritis. | background: a limited number of studies suggest the prevalence of periodontal pathogens in patients with rheumatoid arthritis (ra); however, results are inconsistent. the aim of this study was to investigate clinical periodontal and microbiologic parameters in patients with ra. methods: 66 patients with ra aged 49.5±8.4 years participated in the study. the periodontal situation was assessed with the periodontal screening index (psr(®)/psi); allocated to: healthy, gingivitis (psr(®)/psi score 0-2 ... | 2011 | 21405936 |
| clustering of subgingival microbial species in adolescents with periodontitis. | it has become increasingly recognized that groups of microorganisms interact within the subgingival plaque of adult subjects with periodontitis. it is much less clear, however, whether the consortia of microorganisms associated with periodontitis are different in early and more advanced cases of periodontitis. to investigate this point further, subgingival plaque was collected from six sites in 87 adolescents with periodontitis and 73 controls and the samples were analyzed for the detection of 1 ... | 2011 | 21410554 |
| medical microbiology | at present there are over two dozen genera of gram-negative anaerobic bacilli. in most clinical infections, only the genera bacteroides, prevotella, and fusobacterium need be considered. these genera are prevalent in the body as members of the normal flora (fig.20-1), constituting one-third of the total anaerobic isolates from clinical specimens, and may become involved in infections throughout the body (fig.20-2). within the bacteroides group, b fragilis is the most common pathogen, followed b ... | 1996 | 21413336 |
| four cases of bacteraemia caused by fusobacterium nucleatum in febrile, neutropenic patients. | although bacteraemia caused by obligate anaerobic bacteria is a rare event, this phenomenon will be an emerging problem among oncohaematological patients. we report four cases of bacteraemia caused by fusobacterium nucleatum in febrile, neutropenic patients over a 10 month period. all patients had haematological malignancy and severe neutropenia, and three of them suffered from oral mucositis or oedema of the oral mucosal surfaces, which was the probable portal of entry. all isolated strains wer ... | 2011 | 21415204 |
| microbiologic results after non-surgical erbium-doped:yttrium, aluminum, and garnet laser or air-abrasive treatment of peri-implantitis: a randomized clinical trial. | background: the purpose of this study is to assess clinical and microbiologic effects of the non-surgical treatment of peri-implantitis lesions using either an erbium-doped:yttrium, aluminum, and garnet (er:yag) laser or an air-abrasive subgingival polishing method. methods: in a 6-month clinical trial, 42 patients with peri-implantitis were treated at one time with an er:yag laser or an air-abrasive device. routine clinical methods were used to monitor clinical conditions. baseline and 6-month ... | 2011 | 21417591 |
| novel model for multispecies biofilms that uses rigid gas-permeable lenses. | oral biofilms comprise complex multispecies consortia aided by specific inter- and intraspecies interactions occurring among commensals and pathogenic bacterial species. oral biofilms are primary initiating factors of periodontal disease, although complex multifactorial biological influences, including host cell responses, contribute to the individual outcome of the disease. to provide a system to study initial stages of interaction between oral biofilms and the host cells that contribute to the ... | 2011 | 21421785 |
| [study of putative periodontal pathogens colonies in type 2 diabetes with chronic periodontitis]. | ppurpose: to study the permanent planting of periodontal pathogens in the subgingival plaque of periodontitis patients with type 2 diabetes, and evaluate role of periodontal pathogens in chronic periodontitis and type 2 diabetes. | 2010 | 21431261 |
| structural basis of cooperative ligand binding by the glycine riboswitch. | the glycine riboswitch regulates gene expression through the cooperative recognition of its amino acid ligand by a tandem pair of aptamers. a 3.6 +à crystal structure of the tandem riboswitch from the glycine permease operon of fusobacterium nucleatum reveals the glycine binding sites and an extensive network of interactions, largely mediated by asymmetric a-minor contacts, that serve to communicate ligand binding status between the aptamers. these interactions provide a structural basis for how ... | 2011 | 21439473 |
| mpc-polymer reduces adherence and biofilm formation by oral bacteria. | oral biofilms such as dental plaque cause dental caries and periodontitis, as well as aspiration pneumonia and infectious endocarditis by translocation. hence, the suppression of oral biofilm formation is an issue of considerable importance. mechanical removal, disinfectants, inhibition of polysaccharide formation, and artificial sugar have been used for the reduction of oral biofilm. from the viewpoint of the inhibition of bacterial adherence, we investigated whether aqueous biocompatible 2-met ... | 2011 | 21447697 |
| detection of periodontal bacteria in atheromatous plaques by nested polymerase chain reaction. | background. in recent years, increasing evidence regarding the potential association between periodontal diseases and cardiovascular diseases has been elicited. the available evidence underlines the importance of detecting periodontal pathogens on atheromatous plaques as the first step in demonstrating the causal relationship between both entities. it is the main aim of this investigation to detect periodontitis-associated bacteria from carotid artery atheromatous plaques recovered from patients ... | 2011 | 21453047 |
| the oral commensal, streptococcus gordonii, synergizes with tat protein to induce hiv-1 promoter activation in monocytes/macrophages. | trans-activator of transcription (tat) is an hiv-1 protein essential for viral replication. oral periodontopathogens (e.g. fusobacterium nucleatum) enhance hiv-1ltr promoter activation in monocytes/macrophages in absence of tat; however, some oral commensals fail to trigger this response. we sought to determine the effect of tat on hiv-1ltr promoter activation induced by the representative oral commensal streptococcus gordonii in monocytes/macrophages. s. gordonii enhanced hiv-1ltr reactivation ... | 2011 | 21459369 |
| multispecies communities: interspecies interactions influence growth on saliva as sole nutritional source. | human oral bacteria live in multispecies communities in the biofilm called dental plaque. this review focuses on the interactions of seven species and the ability of each species individually and together with other species to grow on saliva as the sole source of nutrient. community formation in biofilms in flow cells is monitored using species-specific fluorophore-conjugated immunoglobulin g, and images are captured by confocal microscopy. early colonizing veillonellae emerge from this review o ... | 2011 | 21485308 |
| analysis of interspecies adherence of oral bacteria using a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis profiling. | information on co-adherence of different oral bacterial species is important for understanding interspecies interactions within oral microbial community. current knowledge on this topic is heavily based on pariwise coaggregation of known, cultivable species. in this study, we employed a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) to systematically analyze the co-adherence profiles of oral bacterial species, and achieved a more ... | 2011 | 21485313 |
| microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease. | uzel ng, teles fr, teles rp, song xq, torresyap g, socransky ss, haffajee ad. microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease. j clin peridontol 2011; doi: 10.1111/j.1600-051x.2011.01730.x. abstract: aim: to monitor microbial shifts during dental biofilm re-development. materials and methods: supra- and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects at baseline and i ... | 2011 | 21488936 |
| influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces in vitro. | objectives: the aim of this study was to analyse the influence of the microtopography and hydrophilicity of titanium (ti) substrates on initial oral biofilm formation. materials and methods: nine bacterial species belonging to the normal oral microbiota, including: aggregatibacter actinomycetemcomitans, actinomyces israelii, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, parvimonas micra, porphyromonas gingivalis, prevotella intermedia, and streptococcus sanguinis were teste ... | 2011 | 21492236 |
| identification and enzymatic analysis of a novel protein associated with production of hydrogen sulfide and l-serine from l-cysteine in fusobacterium nucleatum subsp. nucleatum atcc 25586. | a third enzyme that produced hydrogen sulfide from l-cysteine was identified in fusobacterium nucleatum subsp. nucleatum. the fn1055 gene was cloned from a cosmid library constructed with the genomic dna of f. nucleatum atcc 25586. despite the database annotation that the product of fn1055 is a cysteine synthase, reverse phase hplc revealed that no l-cysteine was produced in vitro by the purified fn1055 protein; however, the enzyme did produce l-serine. in addition, a cysteine auxotroph, escheri ... | 2011 | 21493682 |
| antigenicity of primary endodontic infection against macrophages by the levels of pge(2) production. | root canal contents are potent stimuli for proinflammatory cytokines involved in apical periodontitis. this study investigated target gram-negative bacterial species and endotoxins in primary endodontic infection with apical periodontitis, determined their antigenicity against macrophages through the levels of pge(2), and evaluated their relationship with clinical findings. | 2011 | 21496656 |
| streptococcus cristatus modulates the fusobacterium nucleatum-induced epithelial interleukin-8 response through the nuclear factor-kappa b pathway. | zhang g, chen r, rudney jd.streptococcus cristatusmodulates thefusobacterium nucleatum-induced epithelial interleukin-8 response through the nuclear factor-kappa b pathway. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01373.x.©2011 john wiley & sons a/s background and objective: we previously reported that the interleukin-8 (il-8) response to fusobacterum nucleatum was attenuated in the presence of streptococcus cristatus. here, we further examined the underlying mechanism(s) involved in ... | 2011 | 21521225 |
| preterm low birthweight and the role of oral bacteria. | preterm and low birthweight (ptlbw) continues to be a major cause of mortality and morbidity across the world. in recent years, maternal periodontal disease has been implicated as a risk factor for adverse pregnancy outcomes. there is conflicting evidence to support such an outcome as illustrated by descriptive, case control and randomised controlled trials involving pregnant women from across the world, using different measurement tools to determine the level of periodontal disease. whilst cons ... | 2010 | 21523222 |
| osteoradionecrosis contains a wide variety of cultivable and non-cultivable bacteria. | direct microscopy, anaerobic culture and dna-dna hybridization have previously demonstrated an association between microorganisms and osteoradionecrosis (orn). the purpose of our study was to use culture independent molecular techniques to detect bacteria in necrotic bone lesions of the mandible after radiation therapy. | 2010 | 21523230 |