Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| high homogeneity of the yersinia pestis fatty acid composition. | the cellular fatty acid compositions of 29 strains of yersinia pestis representing the global diversity of this species have been analyzed by gas-liquid chromatography to investigate the extent of fatty acid polymorphism in this microorganism. after culture standardization, all y. pestis strains studied displayed some major fatty acids, namely, the 12:0, 14:0, 3-oh-14:0, 16:0, 16:1omega9cis, 17:0-cyc, and 18:1omega9trans compounds. the fatty acid composition of the various isolates studied was e ... | 2000 | 10747141 |
| pseudotuberculosis in marmosets, tamarins, and goeldi's monkeys (callithrichidae/callimiconidae) housed at a european zoo. | yersinia pseudotuberculosis is an insidious bacterial infectious agent distributed worldwide and endemic to european countries. it has caused several animal deaths and may threaten the effectiveness of breeding projects for endangered species. in this retrospective study, we examine the prevalence of pseudotuberculosis in jersey zoo (channel islands, u.k.) over a period of 16 yr to obtain information that can be applied to prevent the infection. the efforts made to control the disease through va ... | 1999 | 10749440 |
| superantigen ypma exacerbates the virulence of yersinia pseudotuberculosis in mice. | yersinia pseudotuberculosis, a gram-negative bacterium responsible for enteric and systemic infection in humans, produces a superantigenic toxin designated ypma (y. pseudotuberculosis-derived mitogen). to assess the role of ypma in the pathogenesis of y. pseudotuberculosis, we constructed a superantigen-deficient mutant and compared its virulence in a mouse model of infection to the virulence of the wild-type strain. determination of the survival rate after intravenous (i.v.) bacterial inoculati ... | 2000 | 10768943 |
| an immunoglobulin superfamily-like domain unique to the yersinia pseudotuberculosis invasin protein is required for stimulation of bacterial uptake via integrin receptors. | the binding of the yersinia pseudotuberculosis and yersinia enterocolitica invasin proteins to beta(1) integrin receptors allows internalization of these organisms by cultured cells. the c-terminal 192-residue superdomain of the y. pseudotuberculosis invasin is necessary and sufficient for integrin recognition, while a region located outside, and n-terminal to, this superdomain strongly enhances the efficiency of bacterial uptake. within the enhancer region is a domain called d2 that allows inva ... | 2000 | 10768991 |
| [the multiplication dynamics of pathogenic bacteria in relation to the trophic and temperature cultivation conditions]. | the comparative study of the dynamics of multiplication of listeria monocytogenes and yersinia pseudotuberculosis in organic and synthetic media and in distilled water at temperatures of 37 degrees c and 6 degrees c was carried out. this study revealed that in organic media the multiplication of bacteria was good at 37 degrees c and 6 degrees c. in mineral media and distilled water their multiplication was observed only at 6 degrees c. moreover, conditions necessary for the multiplication of pat ... | 2000 | 10808565 |
| interaction of porin from yersinia pseudotuberculosis with lipopolysaccharides. effect of ionic strength, ph, and divalent cations on the binding parameters. | interaction of the pore-forming protein (porin) from yersinia pseudotuberculosis with s- and r-forms of the endogenous lipopolysaccharide (lps) was studied at various ionic strengths (20-600 mm nacl), concentrations of divalent cations (5-100 mm cacl2, mgcl2), and ph values from 3.0 to 9.0. the interaction of the r-lps with porin has been shown in all experimental conditions to be in consensus with the model suggesting binding at independent sites of two types. s-lps binds to interacting sites o ... | 2000 | 10810188 |
| the response regulator phop is important for survival under conditions of macrophage-induced stress and virulence in yersinia pestis. | the two-component regulatory system phopq has been identified in many bacterial species. however, the role of phopq in regulating virulence gene expression in pathogenic bacteria has been characterized only in salmonella species. we have identified, cloned, and sequenced phop orthologues from yersinia pestis, yersinia pseudotuberculosis, and yersinia enterocolitica. to investigate the role of phop in the pathogenicity of y. pestis, an isogenic phop mutant was constructed by using a reverse-genet ... | 2000 | 10816493 |
| microbial pathogens with impaired ability to acquire host iron. | successful microbial pathogens must be adept in obtaining growth-essential iron from healthy hosts. some potential pathogens, however, are sufficiently impaired in iron acquisition ability so as to be dangerous mainly in hosts with such iron loading conditions as alcoholism, asplenia, hemochromatosis, beta-thalassemia major, or tobacco smoking. the association of six impaired pathogens (capnocytophaga canimorsis, yersinia enterocolitica and y. pseudotuberculosis, vibrio vulnificus, tropheryma wh ... | 2000 | 10831229 |
| structural basis for recognition of the translocated intimin receptor (tir) by intimin from enteropathogenic escherichia coli. | intimin is a bacterial adhesion molecule involved in intimate attachment of enteropathogenic and enterohaemorrhagic escherichia coli to mammalian host cells. intimin targets the translocated intimin receptor (tir), which is exported by the bacteria and integrated into the host cell plasma membrane. in this study we localized the tir-binding region of intimin to the c-terminal 190 amino acids (int190). we have also determined the region's high-resolution solution structure, which comprises an imm ... | 2000 | 10835344 |
| gap activity of the yersinia yope cytotoxin specifically targets the rho pathway: a mechanism for disruption of actin microfilament structure. | the yope cytotoxin of yersinia pseudotuberculosis is an essential virulence determinant that is injected into the eukaryotic target cell via a plasmid-encoded type iii secretion system. injection of yope into eukaryotic cells induces depolymerization of actin stress fibres. here, we show that yope exhibits a gtpase-activating protein (gap) activity and that the presence of yope stimulates downregulation of rho, rac and cdc42 activity. yope has an arginine finger motif showing homology with those ... | 2000 | 10844661 |
| [the intensity of cell receptor catabolism in intestinal yersiniosis and pseudotuberculosis]. | the intensity of the catabolism of cell receptors in yersiniosis was evaluated by the level of r-proteins in the blood sera of 95 patients. the study revealed that the degree of an increase in the level of r-protein depended on the severity and the course of the pathological process was the same in both nosological forms of the disease. the high and stable level of r-proteins was indicative of the possibility of the prolonged and relapsing course of yersiniosis. | 1999 | 10851995 |
| [the effect of fluoroquinolones on the adhesive properties of different plasmidovars of yersinia pseudotuberculosis]. | the effect of fluoroquinolones such as ciprofloxacin, pefloxacin and norfloxacin on adhesion of yersinia pseudotuberculosis was studied. it was shown that the effect of the fluoroquinolones was different: decreasing or increasing. the same effect was also observed in the closely related strains of y. pseudotuberculosis. in the strains not dominating in the polyclonal population the adhesion decreased under the effect of the fluoroquinolones. in the strains of the dominant clones the effect on th ... | 2000 | 10862348 |
| sporadic cases of yersinia pseudotuberculosis serotype 5 infection in shodo island, kagawa prefecture. | 2000 | 10871920 | |
| [the effect of the trophic and temperature conditions of cultivation on lipid synthesis by yersinia pseudotuberculosis]. | the comparative study of the synthesis lipids in y. pseudotuberculosis, depending on the conditions of their cultivation (at different temperatures in mineral media and in media, containing organic compounds), has been carried out. as demonstrated in this study, temperature in the main inducing factor, affecting the synthesis of lipids of definite classes and fatty acids, incorporated into these lipids. during the cultivation of y. pseudotuberculosis in mineral and organic media under the condit ... | 1999 | 10876841 |
| [the biochemical mechanisms of energy support in yersinia pseudotuberculosis cells at a low cultivation temperature]. | the object of the study was the first stage of biological oxidation: the transfer of hydrogen electrons to the components of the respiratory chain of y. pseudotuberculosis cells by nad and nadf, coenzymes of pyridine-dependent dehydrogenases, having labile redox properties. the study revealed that in the low-temperature cultivation of y. pseudotuberculosis an increase in the content of nad and nadf was 1.5- to 2.0-fold greater than that observed at 37 degrees c, which was indicative of the fact ... | 2000 | 10876880 |
| yersinia pestis: an instant species? | 2000 | 10878759 | |
| [rapid indication of yersinia using bentonite]. | a new method is proposed for rapid bacteriological detection of yersinia pseudotuberculosis: utilization of bentonite accumulation media. optimal conditions for multiplication of pathogenic yersinia in bentonite media were determined. the proposed method is more sensitive, rapid, and economic than the known methods. | 2000 | 10878918 |
| clinical challenge. acute necrotizing enteritis with microabscesses due to yersinia pseudotuberculosis. | 2000 | 10884139 | |
| invasion of epithelial cells by yersinia pestis: evidence for a y. pestis-specific invasin. | the causative agent of plague, yersinia pestis, is regarded as being noninvasive for epithelial cells and lacks the major adhesins and invasins of its enteropathogenic relatives yersinia enterocolitica and yersinia pseudotuberculosis. however, there are studies indicating that y. pestis invades and causes systemic infection from ingestive and aerogenic routes of infection. accordingly, we developed a gentamicin protection assay and reexamined invasiveness of y. pestis for hela cells. by optimizi ... | 2000 | 10899851 |
| salmonella exploits caspase-1 to colonize peyer's patches in a murine typhoid model. | salmonella typhimurium invades host macrophages and induces apoptosis and the release of mature proinflammatory cytokines. sipb, a protein translocated by salmonella into the cytoplasm of macrophages, is required for activation of caspase-1 (casp-1, an interleukin [il]-1beta-converting enzyme), which is a member of a family of cysteine proteases that induce apoptosis in mammalian cells. casp-1 is unique among caspases because it also directly cleaves the proinflammatory cytokines il-1beta and il ... | 2000 | 10899911 |
| lcrq/yscm1, regulators of the yersinia yop virulon, are injected into host cells by a chaperone-dependent mechanism. | pathogenic yersinia species employ type iii machines to secrete yopbdr into the extracellular milieu. after attaching to host cells, yersiniae transform the type iii machinery into an injection device and target yopehmnopt into eukaryotic cells. yersinia pseudotuberculosis lcrq is a transcriptional regulator that prevents the expression of yop genes. we report that lcrq is injected into eukaryotic cells. yscm1, the transciptional regulator of yersinia enterocolitica, is also injected into eukary ... | 2000 | 10931323 |
| characterization of the o-antigen gene clusters of yersinia pseudotuberculosis and the cryptic o-antigen gene cluster of yersinia pestis shows that the plague bacillus is most closely related to and has evolved from y. pseudotuberculosis serotype o:1b. | one of the most virulent and feared bacterial pathogens is yersinia pestis, the aetiologic agent of bubonic plague. characterization of the o-antigen gene clusters of 21 serotypes of yersinia pseudotuberculosis and the cryptic o-antigen gene cluster of y. pestis showed that the plague bacillus is most closely related to and has evolved from y. pseudotuberculosis serotype o:1b. the nucleotide sequences of both gene clusters (about 20.5 kb each) were determined and compared to identify the differe ... | 2000 | 10931327 |
| the rhogap activity of the yersinia pseudotuberculosis cytotoxin yope is required for antiphagocytic function and virulence. | a variety of pathogenic bacteria use type iii secretion pathways to translocate virulence proteins into host eukaryotic cells. yope is an important virulence factor that is translocated into mammalian cells via a plasmid-encoded type iii system in yersinia spp. yope action in mammalian cells promotes the disruption of actin filaments, cell rounding and blockage of phagocytosis. it was reported recently that two proteins with sequence similarity to yope, sptp of salmonella typhimurium and exos of ... | 2000 | 10931345 |
| in vitro study of antiyersinious effects of oxadin. | 2000 | 10944792 | |
| apically exposed, tight junction-associated beta1-integrins allow binding and yope-mediated perturbation of epithelial barriers by wild-type yersinia bacteria. | using polarized epithelial cells, primarily mdck-1, we assessed the mode of binding and effects on epithelial cell structure and permeability of yersinia pseudotuberculosis yada-deficient mutants. initially, all bacteria except the invasin-deficient (inv) mutant adhered apically to the tight junction areas. these contact points of adjacent cells displayed beta1-integrins together with tight junction-associated zo-1 and occludin proteins. indeed, beta1-integrin expression was maximal in the tight ... | 2000 | 10948163 |
| periodic culturing of nonculturable yersinia pseudotuberculosis forms. | yersinia pseudotuberculosis survive no longer than 30 days during periodic culturing in nutrient medium at 37 degrees c. our experiments demonstrated transformation of these bacteria into resting (nonculturable) forms after one month in culture. resting cells are more viable than dividing cells and under certain conditions can revert to the initial (proliferative) state. | 2000 | 10977925 |
| [the immunomodulating properties of translam]. | effect of translam--beta-1,3;1,6-d-glucan on some of naturally occurring host protective mechanisms was investigated. it was shown that translam has potent biological activity: it has preventive effect on experimental bacterial infections, it stimulates absorbtion and digestion activity of mononuclear phagocytes, it effects on humoral and cell immunity. translam had mitogenic activity. | 2000 | 10987100 |
| [ecological interactions among yersinia in their common reservoir, the rodent]. | plague, due to yersinia pestis, is still active in various foci in the americas, in africa and asia, whereas it has been absent from europe since the end of the 18th century, after having killed the two-thirds of the continent's inhabitants within four centuries. various hypothesis have been proposed to attempt to explain the spontaneous "eradication" of plague from europe, including the improvement of hygiene and habitat, changes in the rat population and cross-immunity induced by other infecti ... | 1999 | 11000952 |
| a multi-domain protein for beta1 integrin-targeted dna delivery. | the development of effective receptor-targeted nonviral vectors for use in vivo is complicated by a number of technical problems. one of these is the low efficiency of the conjugation procedures used to couple protein ligands to the dna condensing carrier molecules. we have made and characterized a multi-domain protein (spkr)4inv, that is designed to target plasmid dna to beta1 integrins in remodeling tissue. it contains a nonspecific dna-binding domain (spkr)4, a rigid alpha-helical linker, and ... | 2000 | 11001371 |
| the yersinia ser/thr protein kinase ypka/yopo directly interacts with the small gtpases rhoa and rac-1. | pathogenic bacteria of the genus yersinia counteract host defense by interfering with eukaryotic signal transduction pathways. ypka of yersinia pseudotuberculosis shares significant homology with eukaryotic ser/thr protein kinases, is translocated into the host cell and has been shown to be an essential virulence factor in a mouse infection model. in this study, we identify the small gtpases rhoa and rac-1 as eukaryotic binding partners of ypka and its homolog yopo of yersinia enterocolitica. we ... | 2000 | 11018537 |
| acute tubulointerstitial nephritis in 21 japanese children. | we studied 21 japanese children aged 1.2 to 14.9 years with biopsy-proven acute tubulointerstitial nephritis (atin; 5 drug-induced, 7 infection-related, 3 tubulointerstitial nephritis and uveitis syndrome (tinu), and 6 unclassified) for clinical presentation, laboratory findings, and outcome to clarify the clinical features of the entity. all developed acute renal failure with peak bun values from 25 to 164 mg/dl (mean 83 mg/dl) and peak serum creatinine values from 1.5 to 15.1 mg/dl (mean 6.5 m ... | 2000 | 11020016 |
| infection of human enterocyte-like cells with rotavirus enhances invasiveness of yersinia enterocolitica and y. pseudotuberculosis. | mixed infection with rotavirus and either yersinia enterocolitica or y. pseudotuberculosis was analysed in caco-2 cells, an enterocyte-like cell line highly susceptible to these pathogens. results showed an increase of bacterial adhesion and internalisation in rotavirus-infected cells. increased internalisation was also seen with escherichia coli strain hb101 (pri203), harbouring the inv gene from y. pseudotuberculosis, which is involved in the invasion process of host cells. in contrast, the su ... | 2000 | 11023186 |
| a study of the yopd-lcrh interaction from yersinia pseudotuberculosis reveals a role for hydrophobic residues within the amphipathic domain of yopd. | the enteropathogen yersinia pseudotuberculosis is a model system used to study the molecular mechanisms by which gram-negative pathogens translocate effector proteins into target eukaryotic cells by a common type iii secretion machine. of the numerous proteins produced by y. pseudotuberculosis that act in concert to establish an infection, yopd (yersinia outer protein d) is a crucial component essential for yop regulation and yop effector translocation. in this study, we describe the mechanisms ... | 2000 | 11029692 |
| identification of exported bacterial proteins via gene fusions to yersinia pseudotuberculosis invasin. | 2000 | 11036636 | |
| interaction of bacterial endotoxins with chitosan. effect of endotoxin structure, chitosan molecular mass, and ionic strength of the solution on the formation of the complex. | the interaction of endotoxins of different structure (lipopolysaccharides (lps) and lipopolysaccharide-protein complexes (lppc)) with chitosan has been studied. it was shown that the mechanism of interaction is rather complicated and depends on the macromolecular organization of endotoxin as well as on the degree of polymerization of the chitosan. chitosan with molecular mass of 20 kd reveals higher affinity to lps than chitosan with molecular mass of 140 kd. endotoxins with long o-specific chai ... | 2000 | 11042502 |
| uptake pathways of clinical and healthy animal isolates of campylobacter jejuni into int-407 cells. | campylobacter jejuni isolates obtained from human and animal sources showed different invasion levels into human embryonic intestinal (int-407) cells. there was no significant relation between the degree of invasion and cytotoxins production. the depolymerization of both microfilaments by cytochalasin-d and microtubules by colchicine, demecolcine and nocodazole or stabilization of microtubules by paclitaxel reduced the invasiveness of c. jejuni, although microfilament depolymerization showed gre ... | 2000 | 11064267 |
| identification of residues in the n-terminal domain of the yersinia tyrosine phosphatase that are critical for substrate recognition. | yoph is a 468-amino acid protein-tyrosine phosphatase that is produced by pathogenic yersinia species. yoph is translocated into host mammalian cells via a type iii protein secretion system. translocation of yoph into human epithelial cells results in dephosphorylation of p130(cas) and paxillin, disruption of focal adhesions, and inhibition of integrin-mediated bacterial phagocytosis. previous studies have shown that the n-terminal 129 amino acids of yoph comprise a bifunctional domain. this dom ... | 2001 | 11069923 |
| adhesion molecules and gene transfer. | cell adhesion molecules are a large group of molecules involved in a variety of cell-to-cell and cell-to-extra-cellular matrix (ecm) interactions. apart from their cellular function these molecules are exploited by a number of pathogenic micro-organisms as receptors for cell entry. discovery of the use of adhesion molecules for binding and internalisation by naturally occurring pathogens has fuelled much research, in recent years, into the utilisation of these molecules for the targeting and upt ... | 2000 | 11072111 |
| a combination of different polymerase chain reaction (pcr) assays for the presumptive identification of yersinia pestis. | a combination of four polymerase chain reaction (pcr) assays targeting the yersinia pestis-specific plasmoidal genes of the fraction 1 capsular antigen and plasminogen activator/coagulase, the gene of the v antigen of the yersinia virulence plasmid, and the chromosomal 16s rrna gene was evaluated for the identification of y. pestis isolates. all four assays were subjected to the same sample preparation technique, reagents and cycling conditions. eighteen y. pestis, 66 y. pseudotuberculosis, 40 y ... | 2000 | 11075545 |
| disruption of signaling by yersinia effector yopj, a ubiquitin-like protein protease. | homologs of the yersinia virulence effector yopj are found in both plant and animal bacterial pathogens, as well as plant symbionts. these yopj family members were shown to act as cysteine proteases. the catalytic triad of the protease was required for inhibition of the mitogen-activated protein kinase (mapk) and nuclear factor kappab (nf-kappab) signaling in animal cells and for induction of localized cell death in plants. the substrates for yopj were shown to be highly conserved ubiquitin-like ... | 2000 | 11090361 |
| structure-function relationships in bombinins h, antimicrobial peptides from bombina skin secretions. | skin secretions of amphibia of the bombina genus contain two families of antimicrobial peptides, the bombinins (bombinin-like peptides) and the bombinins h (h for hydrophobic and hemolytic). the latter family includes a number of peptides containing a d-amino acid in the second position, in addition to their corresponding all l-isomers. the antimicrobial activity of three pairs of bombinin h isomers, h2/h4, h6/h7 and gh-1d/gh-1l, has been investigated. the first two pairs of peptides were actual ... | 2000 | 11090921 |
| a miniaturised semiautomated system for the identification of yersinia species within the genus yersinia. | commercially available identification systems based on biochemical reactions of bacteria are not suited for typing the species of the genus yersinia (y.) or the biovars (bv) of the species y. enterocolitica. this failure is caused by the limited number of biochemical reactions applied, resulting in the absence of important discriminatory key reactions. the micronaut identification system (merlin, bornheim-hersel) makes use of dried substrates/enzymes reactions in the wells of a 96-well microtitr ... | 2000 | 11109503 |
| specific detection of plasmid bearing yersinia isolates by pcr. | a total of 210 isolates belonging to 9 different species of the genus yersinia (y.) was investigated with three different pcr assays targeting two plasmoidal genes, the yersinia adhesin gene (yada) and the v-antigen gene. the yada pcr assay described in 1995 by blais and phillipe, targeting a y. enterocolitica specific gene region and a newly designed assay targeting the gene region functionally responsible for autoagglutination, were compared. both assays identified the same y. enterocolitica s ... | 2000 | 11109506 |
| the superantigenic toxin of yersinia pseudotuberculosis: a novel virulence factor? | recently, a superantigenic toxin designated ypm (yersinia pseudotuberculosis-derived mitogen) was characterized in the supernatant of y. pseudotuberculosis, a gram-negative bacterium involved in human enteric infection. to assess the role of ypm in pathophysiology of y. pseudotuberculosis, a superantigen-deficient mutant was constructed and its virulence was tested in a murine model of infection and compared with the virulence of the wild-type strain (wt). determination of the survival rate afte ... | 2000 | 11111929 |
| synthesis of lipopolysaccharides in the bacterium yersinia pseudotuberculosis: effect of the pvm82 plasmid and growth temperature. | the composition and structure of lipopolysaccharides (lps) of three isogenic strains of yersinia pseudotuberculosis serovar o:1b (without plasmids (82-) and with plasmids pvm82 (82+) or p57 (57+)) grown at 8 or 37 degrees c were studied by chemical and immunochemical methods, sds-polyacrylamide gel electrophoresis, and 13c-nmr spectroscopy. at the lower temperature, the (82-) and (82+) strains synthesized s-form of lps with similar structure characterized by high acylation and immunochemical act ... | 2000 | 11112843 |
| hrpb2 and hrpf from xanthomonas are type iii-secreted proteins and essential for pathogenicity and recognition by the host plant. | the interaction between the plant pathogen xanthomonas campestris pv. vesicatoria and its host plants is controlled by hrp genes (hypersensitive reaction and pathogenicity), which encode a type iii protein secretion system. among type iii-secreted proteins are avirulence proteins, effectors involved in the induction of plant defence reactions. using non-polar mutants, we investigated the role of 12 hrp genes in the secretion of the avirulence protein avrbs3 from x. c. pv. vesicatoria and a heter ... | 2000 | 11115117 |
| diversity and contribution of the intestinal bacterial community to the development of musca domestica (diptera: muscidae) larvae. | the bacterial diversity in the intestinal tract of musca domestica l. was examined in larvae collected from turkey bedding and corn silage. aerobic culturing yielded 25 bacterial species, including 11 from larvae collected from turkey bedding and 14 from larvae collected from corn silage. providencia rettgeri (hadley, elkins & caldwell) was the only species common to both environments. two mammalian pathogens, yersinia pseudotuberculosis (pfeiffer) and ochrobactrum anthropi (holmes), were isolat ... | 2000 | 11126551 |
| yersinia yope is targeted for type iii secretion by n-terminal, not mrna, signals. | pathogenic yersinia species inject virulence proteins, known as yops, into the cytosol of eukaryotic cells. the injection of yops is mediated via a type iii secretion system. previous studies have suggested that yope is targeted for secretion by two signals. one is mediated by its cognate chaperone yera, whereas the other consists of either the 5' end of yope mrna or the n-terminus of yope. in order to characterize the yope n-terminal/5' mrna secretion signal, the first 11 codons of yope were sy ... | 2001 | 11136471 |
| expression of random peptide fused to invasin on bacterial cell surface for selection of cell-targeting peptides. | the protein invasin expressed on the cell surface of the pathogenic bacteria yersinia pseudotuberculosis mediates the entry of this bacterium into cultured mammalian cells. we have developed a system for expression of random peptides on the cell surface of escherichia coli (e. coli) by creation of a fusion hybrid between a peptide and the invasin protein. the fusion protein constructs consist of part of the outer membrane domain of the invasin protein, six proline spacers, and a decamer of rando ... | 2000 | 11137298 |
| lcrv is a channel size-determining component of the yop effector translocon of yersinia. | delivery of yop effector proteins by pathogenic yersinia across the eukaryotic cell membrane requires lcrv, yopb and yopd. these proteins were also required for channel formation in infected erythrocytes and, using different osmolytes, the contact-dependent haemolysis assay was used to study channel size. channels associated with lcrv were around 3 nm, whereas the homologous pcrv protein of pseudomonas aeruginosa induced channels of around 2 nm in diameter. in lipid bilayer membranes, purified l ... | 2001 | 11169103 |
| enteropathogenic escherichia coli (epec) tir receptor molecule does not undergo full modification when introduced into host cells by epec-independent mechanisms. | enteropathogenic escherichia coli (epec), like many other gram-negative pathogens, encodes a type iii secretion apparatus dedicated to the release of virulence-associated proteins. one such protein, tir, is translocated into host cells, where it is modified by the addition of phosphate groups, resulting in a number of species with distinct molecular mass. one phosphorylation event, on tyrosine residue 474 of tir, does not contribute to shifts in molecular mass but is essential for its actin-nucl ... | 2001 | 11179311 |
| ultrastructure of yersinia pseudotuberculosis inhabiting the soil for a long time. | ultrastructural changes in the population of pathogenic yersinia pseudotuberculosis inhabiting a model soil ecosystem for a long time were studied. changes in the bacteria were mainly adaptive until the 8th month of the experiment, their capacity to binary division was preserved. after 9 months cell structure changed: extracellular amorphous matrix appeared, probably due to increased mucus production. | 2000 | 11182826 |
| pathomorphological characteristics of experimental toxemia induced by thermostable yersinia pseudotuberculosis toxin. | pathogenic properties of thermostable toxin responsible for pathogenicity of yersinia pseudotuberculosis were experimentally studied. the toxin exerted a pronounced polyorgan cytopathogenic effect with predominating degenerative destructive changes and membranolytic effect on cell ultrastructure of parenchymatous organs. the toxin is believed to be directly involved in the development of typical pathomorphological picture of pseudotuberculosis, which confirms its pathogenetic role. | 2000 | 11182834 |
| immunogeneity and structural organisation of some plcr-encoded proteins of yersinia pestis. | a novel method of cultivation of yersinia pestis ev-76 and its isogenic strains km-217 (ppst-;pcad+;pfra-) and km-218 (ppst-;pcad-;pfra-) and careful extraction of y. pestis proteins (ypps) permitted isolation of >35 low ca2+ response plasmid (plcr)-encoded products, some of which are potentially new members of the lcr family. immunisation with each ypp demonstrated that 25-, 54-, 72- and 87-kda ypps provided the highest level of protection in mice challenged with y. pestis virulent strain 231. ... | 2001 | 11192499 |
| [use of poly-beta-oxybutyric acid by yersinia pseudotuberculosis and listeria monocytegenes bacteria at various temperatures]. | a comparative investigation of the intracellular content of poly-beta-hydroxybutyric acid showed that yersinia pseudotuberculosis strains accumulated, on the average, lower amounts of this reserve substance than listeria monocytogenes strains. the intracellular pool of poly-beta-hydroxybutyric acid was responsible for the growth of the bacteria at low temperatures (4-6 degrees c) in the absence of any exogenous carbon and energy source. | 2000 | 11195574 |
| the bacterial protein yopj abrogates multiple signal transduction pathways that converge on the transcription factor creb. | bacterially encoded proteins are known to affect eukaryotic signalling pathways and thus cell growth and differentiation. the enteric pathogen yersinia pseudotuberculosis (yp) can translocate yersinia outer proteins (yops) into eukaryotic cells. recently, mkk proteins have been identified as tentative targets of yopj-mediated inhibition of ligand receptor-dependent signal transduction in mammalian cells. these results prompted us to assess whether multiple signal transduction pathways and their ... | 2000 | 11207579 |
| cas/crk signalling mediates uptake of yersinia into human epithelial cells. | uptake of yersinia pseudotuberculosis into mammalian cells involves engagement of beta1 integrin receptors by the bacterial protein invasin. this triggers a host response that involves tyrosine phosphorylation of proteins and the induction of actin rearrangements that lead to cellular uptake of bacteria. in this report, we show that the focal adhesion protein cas plays an important role in yersinia uptake, and that its function is linked to the phosphorylation-dependent interaction between cas a ... | 2000 | 11207607 |
| [diagnostic value of a novel nutrient medium for isolation and cultivation of pathogens causing enteric yersiniosis and pseudotuberculosis]. | a new nutrient medium for isolation and cultivation of the causative agents of enteric yersiniosis and pseudotuberculosis was found to have advantages over endo medium in its differentiating and inhibiting properties. this medium permitted the easy differentiation of yersinia pseudotuberculosis from y. enterocolitica, as well as from escherichia coli, shigella flexneri, klebsiella pneumoniae, k. rhinoscleromatis, hafnia, enterobacter and citrobacter by color; from proteus inconstans by swarming. ... | 2000 | 11210625 |
| [dna, rna, and protein biosynthesis in cells of yersinia pseudotuberculosis at various cultivation temperatures]. | y. pseudotuberculosis cells cultivated at temperatures of 37 degrees c and 8 degrees c were found to be capable of incorporating exogenic precursors into dna, rna and protein. the linear growth of thymidine incorporation occurred during 8 hours of cultivation at 37 degrees c, then the amount of the incorporated label decreased. at 8 degrees c the level of thymidine incorporation into dna gradually increased for 80 hours and longer, but not reaching the level of incorporation observed at 37 degre ... | 2000 | 11210626 |
| [effect of cultivation temperature on nucleic acid level in bacteria listeria monocytogenes and yersinia pseudotuberculosis]. | the relationship between the multiplication of bacteria, the content of nucleic acid and the specific rate of their growth during their batch cultivation in nutrient broth and mineral medium at temperatures of 37 degrees c and 4-6 degrees c was studied in the causative agents of saprozoonotic infections with l. monocytogenes and y. pseudotuberculosis used as typical representatives of such bacteria. the content of dna was shown to remain practically unchanged after the alteration of cultivation ... | 2000 | 11210627 |
| [treatment of pseudotuberculosis in military personnel (scientific basis and practical recommendations)]. | the efficiency of 22 schemes of etiotropic therapy including treatment courses of different duration and new methods was studied basing on the results of examination of 1376 patients and retrospective analysis of 2008 cases of pseudotuberculosis in servicemen. critical review of literature dealing with this problem was conducted. the causes of contradictory literary data have been revealed. specificity of military service and pseudotuberculosis pathogenesis influencing on disease course and effi ... | 2001 | 11219931 |
| [characteristics of phagocytosis of yersinia pseudotuberculosis with various sets of plasmids]. | the study used the selected aplasmid strain of y. pseudotuberculosis 53 and i-716, which contains plasmids that have molecular weights of 48 and 82 md, and i-727 that has only virulence plasmid pyv48. the study has indicated that the fact that yersinias have pvm82 and pyv48 enhances their resistance to phagocytosis. the y. pseudotuberculosis strains carrying these plasmids suppress the responsiveness of phagocytes, inhibit an "oxidative explosion", lower the activities of superoxide dismutase an ... | 1999 | 11221008 |
| the role of yersinia enterocolitica and yersinia pseudotuberculosis in granulomatous appendicitis: a histologic and molecular study. | granulomatous appendicitis is an enigmatic entity. purported causes include crohn's disease, foreign body reactions, sarcoidosis, and infectious agents; however, most cases remain idiopathic. yersinia enterocolitica (ye) and y. pseudotuberculosis (yp) have been implicated as causes of appendicitis, ileocolitis, and mesenteric adenitis. the authors examined the potential role of ye and yp in granulomatous appendicitis using histologic and molecular methods. forty cases of granulomatous appendicit ... | 2001 | 11257626 |
| yersinia pestis pfra shows biovar-specific differences and recent common ancestry with a salmonella enterica serovar typhi plasmid. | population genetic studies suggest that yersinia pestis, the cause of plague, is a clonal pathogen that has recently emerged from yersinia pseudotuberculosis. plasmid acquisition is likely to have been a key element in this evolutionary leap from an enteric to a flea-transmitted systemic pathogen. however, the origin of y. pestis-specific plasmids remains obscure. we demonstrate specific plasmid rearrangements in different y. pestis strains which distinguish y. pestis bv. orientalis strains from ... | 2001 | 11274119 |
| identification of attenuated yersinia pseudotuberculosis strains and characterization of an orogastric infection in balb/c mice on day 5 postinfection by signature-tagged mutagenesis. | yersinia pseudotuberculosis localizes to the distal ileum, cecum, and proximal colon of the gastrointestinal tract after oral infection. using signature-tagged mutagenesis, we isolated 13 y. pseudotuberculosis mutants that failed to survive in the cecum of mice after orogastric inoculation. twelve of these mutants were also attenuated for replication in the spleen after intraperitoneal infection, whereas one strain, mutated the gene encoding invasin, replicated as well as wild-type bacteria in t ... | 2001 | 11292689 |
| vector competence of musca domestica (diptera: muscidae) for yersinia pseudotuberculosis. | the vector potential of adult house flies. musca domestica l., for yersinia pseudotuberculosis (pfeiffer), a pathogen of domestic animals and humans, was investigated. adult flies were allowed to feed on trypticase soy broth (tsb) containing y. pseudotuberculosis for 6 h and then transferred to sterile containers with sterile tsb as a source of water and nutrients. at 6-h intervals, all flies were transferred to sterile containers with sterile tsb and 10 randomly selected flies were examined for ... | 2001 | 11296844 |
| exoenzyme t of pseudomonas aeruginosa elicits cytotoxicity without interfering with ras signal transduction. | one virulence strategy used by the opportunistic pathogen pseudomonas aeruginosa is to target toxic proteins into eukaryotic cells by a type iii secretion mechanism. two of these proteins, exos and exot, show 75% homology on amino acid level. however, compared with exos, exot exhibits highly reduced adp-ribosylating activity and the role of exot in pathogenesis is poorly understood. to study the biological effect of exot, we used a strategy by which exot was delivered into host cells by the hete ... | 2001 | 11298647 |
| [effect of a culturing method and growth phase on composition of lipopolysaccharides in yersinia pseudotuberculosis]. | the effects of the culturing method (suspension cultures in a liquid nutrient broth or colonies on a solid agarized medium) and the growth phase on the lypopolysaccharide (lps) composition of yersinia pseudotuberculosis (o:ib serovar, strain ks 3058) grown in cold (5 degrees c) were studied. the amount the lps synthesized by cells depended on the bacteria growth phase for both media. the lps acylation degree was constant, whereas the length of the o-specific polysaccharide chain varied with the ... | 2001 | 11357400 |
| isolation of pathogenic yersiniae from wild animals in bulgaria. | pathogenic yersinia strains were isolated between december 1998 and april 1999 from 37 wild animals: rabbit (lepus europeus), boar (sus scrofa scrofa), asiatic jackal (canis aureus), red fox (vulpes vulpes), mouflon (ovis musimon), european river otter (lutra lutra), beech marten (martes foina), polecat (musleta putorius) and wild cat (felis silvestris). it was established that among the wild animals y. enterocolitica strains of serotype 0:3 predominated, accompanied by y. pseudotuberculosis str ... | 2001 | 11393816 |
| effects of culture method and growth phase on free lipid composition of yersinia pseudotuberculosis. | the influence of culture method (free-floating cells in liquid nutrient broth or bacteria attached to agar surface on solid agarized medium of the same formulation) and bacterial age on the composition of free lipids in yersinia pseudotuberculosis (o:ib serovar, strain ks 3058) grown in the cold (5 degrees c) has been investigated. the specific growth rate of the bacteria on solid medium was about threefold less than that in liquid medium. the qualitative composition of phospholipids and fatty a ... | 2001 | 11403649 |
| polar targeting of shigella virulence factor icsa in enterobacteriacae and vibrio. | asymmetric localization is key to the proper function of certain prokaryotic proteins important to virulence, chemotaxis, cell division, development, motility, and adhesion. shigella icsa is localized to the old pole of the bacterium, where it mediates assembly of an actin tail inside infected mammalian cells. icsa (virg) is essential to shigella intracellular motility and virulence. we used translational fusions between portions of icsa and the green fluorescent protein (gfp) to determine the r ... | 2001 | 11481451 |
| clinical and microbiological follow-up of an outbreak of yersinia pseudotuberculosis serotype ib. | yersinia pseudotuberculosis, a food-borne pathogen, causes infection that commonly presents as gastroenteritis and mesenteric lymphadenitis. post-infectious complications include erythema nodosum, reactive arthritis and, less commonly, uveitis and nephritis. six serotypes of y. pseudotuberculosis have been identified, and post-infectious complications have been identified following infection with some, but not all, serotypes. the first recognized outbreak of y. pseudotuberculosis serotype lb occ ... | 2001 | 11515763 |
| a highly specific one-step pcr - assay for the rapid discrimination of enteropathogenic yersinia enterocolitica from pathogenic yersinia pseudotuberculosis and yersinia pestis. | based on differences within the yopt-coding region of yersinia. enterocolitica, y pseudotuberculosis and y pestis, a rapid and sensitive one-step polymerase chain reaction assay with high specificity for pathogenic y enterocolitica was developed. by this method pathogenic isolates of y enterocolitica can be easily identified and discriminated from other members of this genus. the entire coding sequence of the yopt effector gene of y. pseudotuberculosis y36 was determined. | 2001 | 11518333 |
| attributes of gammadelta intraepithelial lymphocytes as suggested by their transcriptional profile. | gammadelta t lymphocytes in the intestinal intraepithelial layer (gammadelta iels) are thought to contribute to immune competence, but their actual function remains poorly understood. here we used dna microarrays to study the gene expression profile of gammadelta iels in a yersinia infection system to better define their roles. to validate this approach, mesenteric lymph node cd8(+) alphabeta t cells were similarly analyzed. the transcription profiles show that, whereas lymph node cd8(+) alphabe ... | 2001 | 11526237 |
| properties of yersinia enterocolitica and yersinia pseudotuberculosis in red blood cell concentrate of different abo groups during 30-day storage at 4 degrees c. | the present study aimed to investigate the psychrophilic properties of yersinia enterocolitica and yersinia pseudotuberculosis as contaminants of donated blood. | 2001 | 11531980 |
| the making of a gradient: icsa (virg) polarity in shigella flexneri. | the generation and maintenance of subcellular organization in bacteria is critical for many cell processes and properties, including growth, structural integrity and, in pathogens, virulence. here, we investigate the mechanisms by which the virulence protein icsa (virg) is distributed on the bacterial surface to promote efficient transmission of the bacterium shigella flexneri from one host cell to another. the outer membrane protein icsa recruits host factors that result in actin filament nucle ... | 2001 | 11532149 |
| [monoclonal antibodies for studying antigens of protein origin in serotyping of yersinia pseudotuberculosis]. | hybridomas producing monoclonal antibodies (mab) to yersinia pseudotuberculosis, serovars i-iv, responsible for serovar appurtenance, were obtained. virtually all mabs reacted with protein antigens in immunoblotting. the only exclusion was mab 3a2 presumably reacting with a glycoprotein epitope of complex structure. variability of y. pseudotuberculosis antigenic structure, depending on culturing temperature, was confirmed. polypeptides with mono- or polydetermined antigenic specificity were dete ... | 2001 | 11534396 |
| [effect of peptide immunomodulator gangleen on the activity of yersinia pseudotuberculosis thermostable toxin in oxidant and antioxidant systems of human leukocytes]. | the ability to correct activity of y. pseudotuberculosis thermoresistant toxin on antioxidative enzymes and on active oxygen forms in neurophiles and mononuclears was investigated. toxin at concentration 0.5 and 2.5 mcg/ml did not change o2- production and activity of superoxidedismutase (sod) and glutathione peroxidase (gp), but significantly enhanced catalase and glutathiont reductase (gr) activity. gangleen at concentration 0.0002-0.2 mcg/ml, when added to incubation medium with toxin, stimul ... | 2001 | 11544746 |
| [effect of organic composition of humic acids on enterobacteria multiplication]. | enterobacteria have been found to be capable of active multiplication in humic acids isolated from bentonite clays containing carbohydrates, lipids and proteins. humic acids fractions have been found to be heterogeneous by their molecular weight and organic composition; consequently, they have been found to produce different influence in the multiplication of bacteria. | 2001 | 11548272 |
| [effect of blue-green alga (cyanobacteria) and their exometabolites on formation of resting forms and variability of yersinia pseudotuberculosis]. | research, carried out with the use of bacteriological methods and polymerase chain reaction, revealed that the transformation of y. pseudotuberculosis, associated with blue-green algae anabaena variabilis, into resting (noncultivable) forms took shorter time than in soil extract containing no algae. the exometabolites of "old" cultures of these algae sharply accelerated the formation of resting y. pseudotuberculosis forms. the influence of the algae and the products of their metabolism was manif ... | 2001 | 11550552 |
| actin-based motility is sufficient for bacterial membrane protrusion formation and host cell uptake. | shigella flexneri replicates in the cytoplasm of host cells, where it nucleates host cell actin filaments at one pole of the bacterial cell to form a 'comet tail' that propels the bacterium through the host's cytoplasm. to determine whether the ability to move by actin-based motility is sufficient for subsequent formation of membrane-bound protrusions and intercellular spread, we conferred the ability to nucleate actin on a heterologous bacterium, escherichia coli. previous work has shown that i ... | 2001 | 11553015 |
| glucose as a growth medium factor regulating lipid composition of yersinia pseudotuberculosis. | effects of glucose and growth temperature on yersinia pseudotuberculosis o:1b serovar lipid composition have been studied. these growth parameters were shown to have drastic effects on biosynthetic processes in the pseudotuberculosis bacteria. the temperature effect is the most universal, extending to cell growth and to free lipid and lipopolysaccharide content and composition; it is most conspicuous in the bacteria cultivated on glucose-containing nutrient broth. the effect of glucose is select ... | 2001 | 11566063 |
| [long-term dynamics and prognosis for tularemia and pseudotuberculosis morbidity in the iaroslavl region]. | the trends and main factors influencing the fluctuations of the levels of tularemia and pseudotuberculosis morbidity in the iaroslavl region were revealed by the methods of mathematical statistics (regress analysis, time-series analysis, etc.). tularemia morbidity was 0.467 +/- 0.216 cases (1950-1997) and pseudotuberculosis morbidity was 0.979 +/- 0.297 cases (1979-1997) per 100,000 of the population. the multiple regression equations permitting the prognostication of tularemia and pseudotubercu ... | 2001 | 11569252 |
| a bacterial type iii secretion system inhibits actin polymerization to prevent pore formation in host cell membranes. | the bacterial pathogen yersinia pseudotuberculosis uses type iii secretion machinery to translocate yop effector proteins through host cell plasma membranes. a current model suggests that a type iii translocation channel is inserted into the plasma membrane, and if yops are not present to fill the channel, the channel will form a pore. we examined the possibility that yops act within the host cell to prevent pore formation. yop- mutants of y.pseudotuberculosis were assayed for pore-forming activ ... | 2001 | 11574469 |
| geographical heterogeneity between far eastern and western countries in prevalence of the virulence plasmid, the superantigen yersinia pseudotuberculosis-derived mitogen, and the high-pathogenicity island among yersinia pseudotuberculosis strains. | yersinia pseudotuberculosis produces novel superantigenic toxins designated ypma (y. pseudotuberculosis-derived mitogen), ypmb, and ypmc and has a pathogenicity island termed hpi (high-pathogenicity island) and r-hpi (the right-hand part of the hpi with truncation in its left-hand part) on the chromosome. analysis of the distribution of these virulence factors allowed for differentiation of species y. pseudotuberculosis into six subgroups, thus reflecting the geographical spread of two main clon ... | 2001 | 11574570 |
| environmental control of invasin expression in yersinia pseudotuberculosis is mediated by regulation of rova, a transcriptional activator of the slya/hor family. | invasin is the primary invasive factor of yersinia pseudotuberculosis that allows efficient internalization into eukaryotic cells. we investigated invasin expression and found that the inv gene is regulated in response to a variety of environmental signals, such as temperature, growth phase, nutrients, osmolarity and ph, and requires the product of rova, a member of the slya/hor transcriptional activator family. the rova gene was found by a genetic complementation strategy that restores temperat ... | 2001 | 11580832 |
| bacterial genomics. a plague o' both your hosts. | 2001 | 11586338 | |
| genome sequence of yersinia pestis, the causative agent of plague. | the gram-negative bacterium yersinia pestis is the causative agent of the systemic invasive infectious disease classically referred to as plague, and has been responsible for three human pandemics: the justinian plague (sixth to eighth centuries), the black death (fourteenth to nineteenth centuries) and modern plague (nineteenth century to the present day). the recent identification of strains resistant to multiple drugs and the potential use of y. pestis as an agent of biological warfare mean t ... | 2001 | 11586360 |
| molecular mechanisms of bacteria induced apoptosis. | interaction of mammalian cells with pathogenic bacteria results in a whole variety of responses in the infected cells including internalization or phagocytosis of the bacterium, release of cytokines, secretion of defensins or production of oxygen radicals. however, recent studies pointed out that many bacteria are able to trigger apoptosis in the host cell. the induction of apoptosis upon infection results from a complex interaction of bacterial proteins with cellular proteins finally mediating ... | 2001 | 11595833 |
| plague and other human infections caused by yersinia species. | with an estimated 100 million victims, pandemically and epidemically occurring plague has been looked upon as a classical scourge of mankind during the last two millenia. without treatment at least 50% of the affected individuals die from infection with yersinia pestis, a bacterium belonging to the family of enterobacteriaceae. the disease takes a fulminant course. after an incubation period of 2-6 days, bubonic plague primarily attacks one group of lymph nodes. the onset of pulmonic plague, tra ... | 2001 | 11596907 |
| [comparative study of immunomodulating effects of natural experimental preparations on phagocytosis of yersinia pseudotuberculosis]. | to correct defects of the body's phagocytic system, the authors used experimental agents, such as vital activity products of plant cells, which were obtained by biotechnology. these included arabinogalactan, syringin, k-212, and dfegp. the study agents activate the oxidative metabolism of macrophages, the production of active forms of oxygen, and thus enhance its bactericidal effect against yersinia pseudotuberculosis. syringin and arabinogalactan produce a more pronounced stimulating macrophage ... | 2001 | 11680372 |
| structure of the yersinia type iii secretory system chaperone syce. | in the type iii secretory system of bacterial pathogens, a large number of sequence-divergent but characteristically small (approximately 14-19 kda), acidic (pi approximately 4-5) chaperone proteins have been identified. we present the 1.74 a resolution crystal structure of the yersinia pseudotuberculosis chaperone syce, whose action in promoting translocation of yope into host macrophages is essential to yersinia pathogenesis. syce, a compact, globular dimer with a novel fold, has two large hyd ... | 2001 | 11685245 |
| [use of immunomodulators isolated from marine invertebrates for reducing the toxic effects of thermostable toxin and lipopolysaccharides from yersinia pseudotuberculosis on a macroorganism]. | the possibility to use immunomodulators isolated from marine invertebrates for the lowering of the toxic effects caused by yersinia pseudotuberculosis thermoresistant toxin and lipopolysaccharide was investigated. effects were evaluated by the animals survival rate in per cent and mice average lifetime after toxin lethal dose injection. it was shown that polypeptide gangleen when compared to timalin as well as glycanes mitilane and strombus had dose-dependent protective effect. these substances ... | 2001 | 11697237 |
| [1,3;1,6-beta-d-glucan translam: results of studying and prospects for application]. | the results of translam chemical structure and biological activity investigation are presented. translam is a new original semisynthetic polysaccharide of marine origin. the preparation demonstrated potent treatment effect in experimental radiation disease. it had preventing effect at experimental bacterial infections, stimulated hematopoiesis, had effect on humoral and cell immunity and on factors of nonspecific organism resistance. | 2001 | 11697238 |
| [immunotropic activity of panaxans--bioglycans isolated from ginseng]. | immunomodulating activity of panaxanes--polysaccharides isolated from the roots and culture of panax ginseng was studied. effects of both preparations were analogous. profilaxy use of panaxanes provided increased resistance to coli-sepsis in mice, increased neutrophiles and macrophages phagocytosis, stimulated humoral and cell immune factors and induced important regulating cytokins--interferone gamma and tumor necrosis factor. | 2001 | 11697239 |
| recombinant yersinia yopt leads to uncoupling of rhoa-effector interaction. | yersinia enterocolitica, yersinia pseudotuberculosis, and yersinia pestis deliver different yop (yersinia outer proteins) effector proteins into mammalian cells by a type iii secretion mechanism. recently, it was shown that yersinia producing yopt leads to disruption of the actin cytoskeleton of hela cells (m. iriarte and g. r. cornelis, mol. microbiol. 29:915-929, 1998). to analyze the molecular mechanism of yopt, we cloned and expressed yopt as a glutathione s-transferase fusion protein. recom ... | 2001 | 11705930 |
| dna adenine methylase is essential for viability and plays a role in the pathogenesis of yersinia pseudotuberculosis and vibrio cholerae. | salmonella strains that lack or overproduce dna adenine methylase (dam) elicit a protective immune response to different salmonella species. to generate vaccines against other bacterial pathogens, the dam genes of yersinia pseudotuberculosis and vibrio cholerae were disrupted but found to be essential for viability. overproduction of dam significantly attenuated the virulence of these two pathogens, leading to, in yersinia, the ectopic secretion of virulence proteins (yersinia outer proteins) an ... | 2001 | 11705940 |
| application of high-density array-based signature-tagged mutagenesis to discover novel yersinia virulence-associated genes. | yersinia pestis, the causative agent of plague, and the enteropathogen yersinia pseudotuberculosis have nearly identical nucleotide similarity yet cause markedly different diseases. to investigate this conundrum and to study yersinia pathogenicity, we developed a high-density oligonucleotide array-based modification of signature-tagged mutagenesis (stm). y. pseudotuberculosis ypiii mutants constructed with the tagged transposons were evaluated in the murine yersiniosis infection model. the dna t ... | 2001 | 11705963 |
| phagocytosis of bacterial pathogens: implications in the host response. | phagocytosis of bacterial pathogens is at the heart of the pathogenesis of infections. pathogens have evolved a large array of strategies to escape the deleterious effect of phagocytosis by professional phagocytes among which avoiding phagocytosis, killing the phagocytes or surviving inside them are the most 'popular' solutions. bacterial pathogens are also using induction of phagocytic entry into non-professional phagocytic cells, such as epithelial cells, as a strategy of survival and multipli ... | 2001 | 11708894 |
| alterations in substrate-inhibitor specificity of cholinesterase from the pseudotuberculosic microorganism yersenia pseudotuberculosis as an index of temperature adaptation. | 2001 | 11712166 |