Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| optimized use of the maldi biotyper system and the filmarray bcid panel for direct identification of microbial pathogens from positive blood cultures. | despite the current reliance on blood cultures (bcs), the diagnosis of bloodstream infections (bsis) can be sped up using new technologies performed directly on positive bc bottles. two methods (the maldi biotyper system and filmarray blood culture identification [bcid] panel) are potentially applicable. in this study, we performed a large-scale clinical evaluation (1,585 microorganisms from 1,394 bsi episodes) on the combined use of the maldi biotyper and filmarray bcid panel compared to a refe ... | 2016 | 26677254 |
| haemolysis as a first sign of thromboembolic event and acute pump thrombosis in patients with the continuous-flow left ventricular assist device heartmate ii. | despite advances in pump technology, thromboembolic events/acute pump thrombosis remain potentially life-threatening complications in patients with continuous-flow left ventricular assist devices (cf-lvad). we sought to determine early signs of thromboembolic event/pump thrombosis in patients with cf-lvad, which could lead to earlier intervention. | 2015 | 26689927 |
| haemolysis as a first sign of thromboembolic event and acute pump thrombosis in patients with the continuous-flow left ventricular assist device heartmate ii. | despite advances in pump technology, thromboembolic events/acute pump thrombosis remain potentially life-threatening complications in patients with continuous-flow left ventricular assist devices (cf-lvad). we sought to determine early signs of thromboembolic event/pump thrombosis in patients with cf-lvad, which could lead to earlier intervention. | 2015 | 26689927 |
| purification, characterization of l-methioninase from candida tropicalis, and its application as an anticancer. | the aim of the present study is to purify l-methioninase from candida tropicalis 34.19-fold with 27.98% recovery after ion exchange chromatography followed by gel filtration. the purified enzyme revealed a single band on sds-page gel with a molecular weight of 46 kda. its optimum temperature was 45 to 55 and thermal stability was 55°c for 15 min. the enzyme had optimum ph at 6.5 and stability at a ph range of 5.5 to 7.0 for 24 hr. the maximum activity was observed with substrate concentration of ... | 2015 | 26691554 |
| copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines. | natural products display numerous therapeutic properties (e.g., antibacterial activity), providing the population with countless benefits. therefore, the search for novel biologically active, naturally occurring compounds is extremely important. the present paper describes the antibacterial action of the copaifera langsdorffii oleoresin and ten compounds isolated from this oleoresin against multiresistant bacteria; it also reports the antiproliferative activity of the copaifera langsdorffii oleo ... | 2015 | 26691920 |
| draft genome sequence of citrobacter freundii strain st2, a γ-proteobacterium that produces n-acylhomoserine lactones. | citrobacter freundii strain st2, isolated from the algae bloom sample, possesses an n-acylhomoserine lactone (ahl) production activity that secretes short-chain ahl molecules. in this study, we sequenced the complete genome of c. freundii strain st2 to understand the molecular regulation of the ahl system and to search for the ahl gene in this bacterium. the results show that the genome size is 4.89 mb with a g + c content of 51.96%. 4626 function proteins were predicted and 3647 proteins were a ... | 2015 | 26697383 |
| prevalence and antimicrobial resistance of microbes causing bloodstream infections in unguja, zanzibar. | bloodstream infections (bsi) are frequent and cause high case-fatality rates. urgent antibiotic treatment can save patients' lives, but antibiotic resistance can render antibiotic therapy futile. this study is the first to collect epidemiological data on bsi from unguja, zanzibar. | 2015 | 26700032 |
| mechanism of the appabluf photocycle probed by site-specific incorporation of fluorotyrosine residues: effect of the y21 pka on the forward and reverse ground-state reactions. | the transcriptional antirepressor appa is a blue light using flavin (bluf) photoreceptor that releases the transcriptional repressor ppsr upon photoexcitation. light activation of appa involves changes in a hydrogen-bonding network that surrounds the flavin chromophore on the nanosecond time scale, while the dark state of appa is then recovered in a light-independent reaction with a dramatically longer half-life of 15 min. residue y21, a component of the hydrogen-bonding network, is known to be ... | 2016 | 26708408 |
| kpc-like carbapenemase-producing enterobacteriaceae colonizing patients in europe and israel. | in a 2008-2011 survey, 17,945 patients in 18 hospital units in europe and israel were screened for carriage of klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteriaceae, resulting in identification of 124 positive patients. the isolates were dominated by klebsiella pneumoniae sequence type 258 (st258) kpc-2 and st512 kpc-3, mainly from greece and italy, respectively, whereas israeli isolates were of diverse species, clones, and kpc variants. various blakpc platforms were observed, a ... | 2016 | 26711772 |
| detection of quiescent infections with multiple elephant endotheliotropic herpesviruses (eehvs), including eehv2, eehv3, eehv6, and eehv7, within lymphoid lung nodules or lung and spleen tissue samples from five asymptomatic adult african elephants. | more than 80 cases of lethal hemorrhagic disease associated with elephant endotheliotropic herpesviruses (eehvs) have been identified in young asian elephants worldwide. diagnostic pcr tests detected six types of eehv in blood of elephants with acute disease, although eehv1a is the predominant pathogenic type. previously, the presence of herpesvirus virions within benign lung and skin nodules from healthy african elephants led to suggestions that african elephants may be the source of eehv disea ... | 2016 | 26719245 |
| triton hodge test: improved protocol for modified hodge test for enhanced detection of ndm and other carbapenemase producers. | accurate detection of carbapenemase-producing gram-negative bacilli is of utmost importance for the control of nosocomial spread and the initiation of appropriate antimicrobial therapy. the modified hodge test (mht), a carbapenem inactivation assay, has shown poor sensitivity in detecting the worldwide spread of new delhi metallo-β-lactamase (ndm). recent studies demonstrated that ndm is a lipoprotein anchored to the outer membrane in gram-negative bacteria, unlike all other known carbapenemases ... | 2016 | 26719442 |
| are atrophic long-bone nonunions associated with low-grade infections? | impaired fracture healing, especially when associated with bacterial infection, is a severe complication following long-bone fractures and requires special treatment. because standard diagnostic techniques might provide falsely negative results, we evaluated the sonication method for detection of bacteria on implants of patients with fracture nonunions. a total of 49 patients with a nonunion (group nu) and, for comparison, 45 patients who had undergone routine removal of osteosynthetic material ... | 2015 | 26719698 |
| dissemination of carbapenemases producing gram negative bacteria in the middle east. | the emergence and spread of carbapenemase-producing bacteria, that hydolyze most β-lactams, including carbapenems, are a major concern of public health system worldwide, particularly in the middle east area. since the plasmids harboring resistance genes could be spread across other bacterial populations, detection of carbapenemase-producing organisms has become more problematic. these organisms produce different types of enzymes including the most prevalent types including kpc, vim, imp, ndm, an ... | 2015 | 26719779 |
| effects of operating parameters on measurements of biochemical oxygen demand using a mediatorless microbial fuel cell biosensor. | the conventional biochemical oxygen demand (bod) method takes five days to analyze samples. a microbial fuel cell (mfc) may be an alternate tool for rapid bod determination in water. however, a mfc biosensor for continuous bod measurements of water samples is still unavailable. in this study, a mfc biosensor inoculated with known mixed cultures was used to determine the bod concentration. effects of important parameters on establishing a calibration curve between the bod concentration and output ... | 2015 | 26729113 |
| effects of operating parameters on measurements of biochemical oxygen demand using a mediatorless microbial fuel cell biosensor. | the conventional biochemical oxygen demand (bod) method takes five days to analyze samples. a microbial fuel cell (mfc) may be an alternate tool for rapid bod determination in water. however, a mfc biosensor for continuous bod measurements of water samples is still unavailable. in this study, a mfc biosensor inoculated with known mixed cultures was used to determine the bod concentration. effects of important parameters on establishing a calibration curve between the bod concentration and output ... | 2015 | 26729113 |
| klebsiella pneumoniae: development of carbapenem resistance due to acquisition of bla ndm-1 during antimicrobial therapy in twin infants with pneumonia. | to identify the mechanism of in vivo development of carbapenem resistance in klebsiella pneumoniae. | 2015 | 26733952 |
| a plasmid-transposon hybrid mutagenesis system effective in a broad range of enterobacteria. | random transposon mutagenesis is a powerful technique used to generate libraries of genetic insertions in many different bacterial strains. here we develop a system facilitating random transposon mutagenesis in a range of different gram-negative bacterial strains, including pectobacterium atrosepticum, citrobacter rodentium, serratia sp. atcc39006, serratia plymuthica, dickeya dadantii, and many more. transposon mutagenesis was optimized in each of these strains and three studies are presented t ... | 2015 | 26733980 |
| structural basis for latency and function of immune inhibitor a metallopeptidase, a modulator of the bacillus anthracis secretome. | immune inhibitor a(inha)-type metallopeptidases are potential virulence factors secreted by members of the bacillus cereus group. two paralogs from anthrax-causing bacillus anthracis (bainha1 and bainha2) were shown to degrade host tissue proteins with broad substrate specificity. analysis of their activation mechanism and the crystal structure of a zymogenic bainha2 variant revealed a ∼750-residue four-domain structure featuring a pro-peptide, a catalytic domain, a domain reminiscent of viral e ... | 2016 | 26745529 |
| significance and roles of proteus spp. bacteria in natural environments. | proteus spp. bacteria were first described in 1885 by gustav hauser, who had revealed their feature of intensive swarming growth. currently, the genus is divided into proteus mirabilis, proteus vulgaris, proteus penneri, proteus hauseri, and three unnamed genomospecies 4, 5, and 6 and consists of 80 o-antigenic serogroups. the bacteria are known to be human opportunistic pathogens, isolated from urine, wounds, and other clinical sources. it is postulated that intestines are a reservoir of these ... | 2016 | 26748500 |
| structure of methionine γ-lyase from clostridium sporogenes. | methionine γ-lyase (mgl) is a pyridoxal 5'-phosphate-dependent enzyme that catalyzes the γ-elimination reaction of l-methionine. the enzyme is a promising target for therapeutic intervention in some anaerobic pathogens and has attracted interest as a potential cancer treatment. the crystal structure of mgl from clostridium sporogenes has been determined at 2.37 å resolution. the fold of the protein is similar to those of homologous enzymes from citrobacter freundii, entamoeba histolytica, pseudo ... | 2016 | 26750487 |
| urosepsis--etiology, diagnosis, and treatment. | sepsis is among the most common causes of death in germany. urosepsis accounts for 9-31% of all cases and has a mortality of 20-40%, which is low compared with that of sepsis in general. as the population ages, the incidence of urosepsis is likely to rise. | 2015 | 26754121 |
| effects of egg shell membrane hydrolysates on anti-inflammatory, anti-wrinkle, anti-microbial activity and moisture-protection. | this study was conducted to examine the effects of eggshell membrane hydrolysates (esmh) on the anti-inflammatory, anti-wrinkle, anti-microbial activity, and moisture-protection for cosmetic use. whole esmh (before fractionation), and fraction i (>10 kda), fraction ii (3-10 kda), and fraction iii (<3 kda) of the hydrolysates were assessed in this experiment. as lipopolysaccharide (lps) and ifn-γ caused the inflammation on raw264.7 cell, whole esmh and fraction i showed to be effective in inhibit ... | 2014 | 26760742 |
| comparison of culture, conventional and real-time pcr methods for listeria monocytogenes in foods. | we compared standard culture methods as well as conventional pcr and real-time pcr for the detection of listeria monocytogenes (l. monocytogenes) in milk, cheese, fresh-cut vegetables, and raw beef that have different levels of background microflora. no statistical differences were observed in sensitivity between the two selective media in all foods. in total, real-time pcr assay exhibited statistically excellent detection sensitivity (p<0.05) and was less time consuming and laborious as compare ... | 2014 | 26761501 |
| distribution channel and microbial characteristics of pig by-products in korea. | the distribution channel of meat by-products from the pig farm to the final consumer can include a meat processor, wholesale market, wholesaler, retailer, and butcher shop. bacterial contamination at any of these steps remains to be a serious public health concern. the aim of this study was to evaluate the distribution channel and microbial characteristics of pig by-products in korea. upon evaluation of pig by-products in cold storage, we found that the small and large intestine were significant ... | 2014 | 26761676 |
| automated broad-range molecular detection of bacteria in clinical samples. | molecular detection methods, such as quantitative pcr (qpcr), have found their way into clinical microbiology laboratories for the detection of an array of pathogens. most routinely used methods, however, are directed at specific species. thus, anything that is not explicitly searched for will be missed. this greatly limits the flexibility and universal application of these techniques. we investigated the application of a rapid universal bacterial molecular identification method, is-pro, to rout ... | 2016 | 26763956 |
| synthesis and antibacterial characterization of sustainable nanosilver using naturally-derived macromolecules. | greener nanosynthesis utilizes fewer amounts of materials, water, and energy; while reducing or replacing the need for organic solvents. a novel approach is presented using naturally-derived flavonoids including quercetin pentaphosphate (qpp), quercetin sulfonic acid (qsa) and apigenin triphosphate (atrp). these water soluble, phosphorylated flavonoids were utilized both as reducing agent and stabilizer. the synthesis was achieved at room temperature using water as a solvent and it requires no c ... | 2016 | 26765510 |
| essential oils to control ichthyophthiriasis in pacu, piaractus mesopotamicus (holmberg): special emphasis on treatment with melaleuca alternifolia. | in vitro effect of the melaleuca alternifolia, lavandula angustifolia and mentha piperita essential oils (eos) against ichthyophthirius multifiliis and in vivo effect of m. alternifolia for treating ichthyophthiriasis in one of the most important south american fish, piaractus mesopotamicus (holmberg), were evaluated. the in vitro test consisted of three eos, each at concentrations of 57 μl l(-1) , 114 μl l (-1) , 227 μl l(-1) and 455 μl l (-1) , which were assessed once an hour for 4 h in micro ... | 2016 | 26776242 |
| antimicrobial activities of bacteriocins e 50-52 and b 602 against antibiotic-resistant strains involved in nosocomial infections. | the antimicrobial spectra of previously published bacteriocin e 50-52 (39 a.a.; 3,932 da; pi = 8.5) and bacteriocin b 602 (29 a.a.; 3,864 da; pi = 7.2) were determined. named peptides were related to class iia (pediocin-like) bacteriocins. minimal inhibitory concentrations (mics) of bacteriocins have been determined for bacterial isolates that were causative agents of nosocomial infections collected from russian hospitals in 2003-2007, namely methicillin-resistant staphylococcus aureus (mrsa) (n ... | 2009 | 26783168 |
| identification of phenolic compounds and evaluation of antioxidant and antimicrobial properties of euphorbia tirucalli l. | bioactive compounds extracted from natural sources can benefit human health. the aim of this work was to determine total phenolic content and antioxidant activity in extracts of euphorbia tirucalli l. followed by identification and quantification of the phenolic compounds, as well as their antibacterial activities. antioxidant activities were determined by dpph and abts(•+) assay. identification of phenolic compounds was performed using high-performance liquid chromatography (hplc), and antimicr ... | 2014 | 26784670 |
| genomic characterization of enterobacter cloacae isolates from china that coproduce kpc-3 and ndm-1 carbapenemases. | here, we report twoenterobacter cloacaesequence type 231 isolates coproducing kpc-3 and ndm-1 that have caused lethal infections in a tertiary hospital in china. theblandm-1-harboring plasmids carry inca/c2and incr replicons, showing a mosaic plasmid structure, and theblandm-1is harbored on a novel class i integron-like element.blakpc-3is located on a tn3-δblatem-1-blakpc-3-δtn1722element, flanked by two 9-bp direct-repeat sequences and harbored on an incx6 plasmid. | 2016 | 26787700 |
| histamine development and bacterial diversity in microbially-challenged tonggol (thunnus tonggol) under temperature abuse during canning manufacture. | histamine formation and bacteriological changes caused by temperature abuse commonly occurring in the manufacturing process of standard canned tuna was assessed in microbiologically challenged tonggol (thunnus tonggol). the in situ challenge was performed by water-soaking at 26-28 °c for 7 h to ensure the multiplication and active phase of fish microflora. right after pre-cooking to back-bone temperature (bbt) of 50-52 °c, histamine dropped to 5.17 ± 2.71 ppm, and slowly reached 6.84 ± 1.69 ppm ... | 2015 | 26787946 |
| histamine development and bacterial diversity in microbially-challenged tonggol (thunnus tonggol) under temperature abuse during canning manufacture. | histamine formation and bacteriological changes caused by temperature abuse commonly occurring in the manufacturing process of standard canned tuna was assessed in microbiologically challenged tonggol (thunnus tonggol). the in situ challenge was performed by water-soaking at 26-28 °c for 7 h to ensure the multiplication and active phase of fish microflora. right after pre-cooking to back-bone temperature (bbt) of 50-52 °c, histamine dropped to 5.17 ± 2.71 ppm, and slowly reached 6.84 ± 1.69 ppm ... | 2015 | 26787946 |
| development of molecular approach based on pcr assay for detection of histamine producing bacteria. | histamine fish poisoning becomes highly concern not only in public health but also economic aspect. histamine is produced from histidine in fish muscles by bacterial decarboxylase enzyme. several techniques have been developed to determine the level of histamine in fish and their products but the effective method for detecting histamine producing bacteria is still required. this study was attempted to detect histamine producing bacteria by newly developed pcr condition. histamine producing bacte ... | 2015 | 26787983 |
| development of molecular approach based on pcr assay for detection of histamine producing bacteria. | histamine fish poisoning becomes highly concern not only in public health but also economic aspect. histamine is produced from histidine in fish muscles by bacterial decarboxylase enzyme. several techniques have been developed to determine the level of histamine in fish and their products but the effective method for detecting histamine producing bacteria is still required. this study was attempted to detect histamine producing bacteria by newly developed pcr condition. histamine producing bacte ... | 2015 | 26787983 |
| performances and reliability of bruker microflex lt and vitek ms maldi-tof mass spectrometry systems for the identification of clinical microorganisms. | in clinical microbiology laboratories, routine microbial identification is mostly performed using culture based methodologies requiring 24 to 72 hours from culturing to identification. matrix assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) technology has been established as a cost effective, reliable, and faster alternative identification platform. in this study, we evaluated the reliability of the two available maldi-tof ms systems for their routine clinical ... | 2015 | 26793718 |
| sulfoxides, analogues of l-methionine and l-cysteine as pro-drugs against gram-positive and gram-negative bacteria. | the problem of resistance to antibiotics requires the development of new classes of broad-spectrum antimicrobial drugs. the concept of pro-drugs allows researchers to look for new approaches to obtain effective drugs with improved pharmacokinetic and pharmacodynamic properties. thiosulfinates, formed enzymatically from amino acid sulfoxides upon crushing cells of genus allium plants, are known as antimicrobial compounds. the instability and high reactivity of thiosulfinates complicate their use ... | 2015 | 26798500 |
| the salmonella in silico typing resource (sistr): an open web-accessible tool for rapidly typing and subtyping draft salmonella genome assemblies. | for nearly 100 years serotyping has been the gold standard for the identification of salmonella serovars. despite the increasing adoption of dna-based subtyping approaches, serotype information remains a cornerstone in food safety and public health activities aimed at reducing the burden of salmonellosis. at the same time, recent advances in whole-genome sequencing (wgs) promise to revolutionize our ability to perform advanced pathogen characterization in support of improved source attribution a ... | 2016 | 26800248 |
| prospective study using anterior approach did not show association between modic 1 changes and low grade infection in lumbar spine. | the modern literature is producing a rapidly growing number of articles which highlight the relationship between infection and lumbar disc degeneration. however, the means by which samples are collected is questionable. posterior approach surgery is not free from skin contamination. the possibility of intraoperative contamination of disc biopsies cannot be excluded. | 2016 | 26818032 |
| direct identification of urinary tract pathogens from urine samples, combining urine screening methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry. | early diagnosis of urinary tract infections (utis) is essential to avoid inadequate or unnecessary empirical antibiotic therapy. microbiological confirmation takes 24 to 48 h. the use of screening methods, such as cytometry and automated microscopic analysis of urine sediment, allows the rapid prediction of negative samples. in addition, matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is a widely established technique in clinical microbiology laborator ... | 2016 | 26818668 |
| serum bactericidal assay: new role in salmonella detection. | while inspecting animal feed for salmonella contamination, we routinely observed bacterial colonies on selective agars that were similar in appearance to those formed by salmonella. these were identified as citrobacter freundii, proteus mirabilis, and serratia fonticola using biochemical and serological techniques. because the presence of these bacterial species confounds identification of salmonella, we refer to them as "interference bacteria." polyvalent antisera against these interference bac ... | 2017 | 26822285 |
| a multi-substrate approach for functional metagenomics-based screening for (hemi)cellulases in two wheat straw-degrading microbial consortia unveils novel thermoalkaliphilic enzymes. | functional metagenomics is a promising strategy for the exploration of the biocatalytic potential of microbiomes in order to uncover novel enzymes for industrial processes (e.g. biorefining or bleaching pulp). most current methodologies used to screen for enzymes involved in plant biomass degradation are based on the use of single substrates. moreover, highly diverse environments are used as metagenomic sources. however, such methods suffer from low hit rates of positive clones and hence the dis ... | 2016 | 26822785 |
| high yield 1,3-propanediol production by rational engineering of the 3-hydroxypropionaldehyde bottleneck in citrobacter werkmanii. | imbalance in cofactors causing the accumulation of intermediates in biosynthesis pathways is a frequently occurring problem in metabolic engineering when optimizing a production pathway in a microorganism. in our previous study, a single knock-out citrobacter werkmanii ∆dhad was constructed for improved 1,3-propanediol (pdo) production. instead of an enhanced pdo concentration on this strain, the gene knock-out led to the accumulation of the toxic intermediate 3-hydroxypropionaldehyde (3-hpa). t ... | 2016 | 26822953 |
| development of a new real-time pcr system for simultaneous detection of bacteria and fungi in pathological samples. | a novel system for simultaneous detection of pathogenic bacteria and fungi in pathological samples was developed using a real-time polymerase chain reaction (pcr) system. this system, designated the "multi-microbial real-time pcr", has the potential to simultaneously detect 68 bacterial and 9 fungal species in a 96-well plate format. all probe-primer sets were designed to produce amplicons smaller than 210 bp using formalin-fixed paraffin-embedded samples as input. the specificity and sensitivit ... | 2015 | 26823918 |
| colistin-nonsusceptible pseudomonas aeruginosa sequence type 654 with blandm-1 arrives in north america. | this study describes 3 different blandm-1 genetic platforms in 3 different species obtained from the same patient who was directly transferred to an institution in calgary, alberta, canada, following a prolonged hospital stay in india. the blandm-1 in the escherichia coli isolate was located on a 176-kb inca/c plasmid contained within an iscr1 region. the blandm-1 in the providencia rettgeri isolate was located on a 117-kb inct plasmid contained within tn3000, while the blandm-1 in the pseudomon ... | 2016 | 26824951 |
| prevalence of urinary tract infection and antimicrobial susceptibility among diabetic patients with controlled and uncontrolled glycemia in kuwait. | diabetic patients have higher risk of urinary tract infection (uti). in the present study, we investigated the impact of glycemic control in diabetic patients on uti prevalence, type of strains, and their antimicrobial drugs susceptibility. this study was conducted on urine samples from 722 adult diabetic patients from which 252 (35%) samples were positive for uropathogens. most uti cases occurred in the uncontrolled glycemic group (197 patients) versus 55 patients with controlled glycemia. high ... | 2015 | 26844231 |
| prevalence of urinary tract infection and antimicrobial susceptibility among diabetic patients with controlled and uncontrolled glycemia in kuwait. | diabetic patients have higher risk of urinary tract infection (uti). in the present study, we investigated the impact of glycemic control in diabetic patients on uti prevalence, type of strains, and their antimicrobial drugs susceptibility. this study was conducted on urine samples from 722 adult diabetic patients from which 252 (35%) samples were positive for uropathogens. most uti cases occurred in the uncontrolled glycemic group (197 patients) versus 55 patients with controlled glycemia. high ... | 2015 | 26844231 |
| plasmid pec156, a naturally occurring escherichia coli genetic element that carries genes of the ecoviii restriction-modification system, is mobilizable among enterobacteria. | type ii restriction-modification systems are ubiquitous in prokaryotes. some of them are present in naturally occurring plasmids, which may facilitate the spread of these systems in bacterial populations by horizontal gene transfer. however, little is known about the routes of their dissemination. as a model to study this, we have chosen an escherichia coli natural plasmid pec156 that carries the ecoviii restriction modification system. the presence of this system as well as the cis-acting cer s ... | 2016 | 26848973 |
| visual and efficient immunosensor technique for advancing biomedical applications of quantum dots on salmonella detection and isolation. | it is a great challenge in nanotechnology for fluorescent nanobioprobes to be applied to visually detect and directly isolate pathogens in situ. a novel and visual immunosensor technique for efficient detection and isolation of salmonella was established here by applying fluorescent nanobioprobes on a specially-designed cellulose-based swab (a solid-phase enrichment system). the selective and chromogenic medium used on this swab can achieve the ultrasensitive amplification of target bacteria and ... | 2016 | 26853517 |
| consistent 1,3-propanediol production from glycerol in mixed culture fermentation over a wide range of ph. | glycerol is currently an over-produced chemical that can be used as substrate for the production of high value products such as 1,3-propanediol (1,3-pdo) in fermentation processes. the aim of this study was to investigate the effect of initial ph on a batch mixed culture fermentation of glycerol, considering both the bacterial community composition and the fermentation patterns. | 2016 | 26855671 |
| antimicrobial activities of ceftaroline and comparator agents against bacterial organisms causing bacteremia in patients with skin and skin structure infections in u.s. medical centers, 2008 to 2014. | we evaluated the antimicrobial susceptibility of 1,454 organisms consecutively collected from patients with bacteremia associated with skin and skin structure infections. the most common organisms obtained werestaphylococcus aureus(670 organisms [46.1%]),escherichia coli(200 organisms [13.8%]), β-hemolytic streptococci (βhs) (138 organisms [9.5%]), andklebsiella pneumoniae(109 organisms [7.5%]). the susceptibility rates for ceftaroline were 97.9% fors. aureus(95.9% among methicillin-resistants. ... | 2016 | 26856825 |
| a novel pcr-based approach for accurate identification of vibrio parahaemolyticus. | a pcr-based assay was developed for more accurate identification of vibrio parahaemolyticus through targeting the bla carb-17 like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. homologous analysis showed that bla carb-17 like genes were more conservative than the tlh, toxr and atpa genes, the genetic markers commonly used as detection targets in identification of v. parahaemolyticus. our data showed that this bla car ... | 2016 | 26858713 |
| persistence of nasal colonization with human pathogenic bacteria and associated antimicrobial resistance in the german general population. | the nares represent an important bacterial reservoir for endogenous infections. this study aimed to assess the prevalence of nasal colonization by different important pathogens, the associated antimicrobial susceptibility and risk factors. we performed a prospective cohort study among 1878 nonhospitalized volunteers recruited from the general population in germany. participants provided nasal swabs at three time points (each separated by 4-6 months). staphylococcus aureus, enterobacteriaceae and ... | 2015 | 26862431 |
| persistence of nasal colonization with human pathogenic bacteria and associated antimicrobial resistance in the german general population. | the nares represent an important bacterial reservoir for endogenous infections. this study aimed to assess the prevalence of nasal colonization by different important pathogens, the associated antimicrobial susceptibility and risk factors. we performed a prospective cohort study among 1878 nonhospitalized volunteers recruited from the general population in germany. participants provided nasal swabs at three time points (each separated by 4-6 months). staphylococcus aureus, enterobacteriaceae and ... | 2015 | 26862431 |
| antimicrobial susceptibility and molecular epidemiology of extended-spectrum beta-lactamase-producing enterobacteriaceae from intensive care units at hamad medical corporation, qatar. | the emergence of extended-spectrum beta-lactamase (esbl)-producing isolates has important clinical and therapeutic implications. high prevalence of esbl-producing enterobacteriaceae has been reported in the literature for clinical samples from a variety of infection sites. the present study was undertaken to evaluate the prevalence of esbl-producing enterobacteriaceae, and to perform molecular characterization and antimicrobial susceptibility testing of clinical isolates from patients admitted t ... | 2016 | 26865975 |
| epidemiology and burden of bloodstream infections caused by extended-spectrum beta-lactamase producing enterobacteriaceae in a pediatric hospital in senegal. | severe bacterial infections are not considered as a leading cause of death in young children in sub-saharan africa. the worldwide emergence of extended-spectrum beta-lactamase producing enterobacteriaceae (esbl-e) could change the paradigm, especially in neonates who are at high risk of developing healthcare-associated infections. | 2016 | 26867226 |
| prevalence of quinolone resistance genes among extended-spectrum b-lactamase-producing escherichia coli in mashhad, iran. | escherichia coli is an important bacterial species based on incidence and associated infection severity. some e. coli strains produce extended-spectrum beta lactamase (esbl) and are called esbl-producing e. coli. these strains are resistant to most classes of cephalosporin and a number of other classes of antibiotics. plasmids carrying qnr genes have been found to transmit quinolone resistance. | 2015 | 26870307 |
| characteristics of extended-spectrum β-lactamase (esbl)- and pampc beta-lactamase-producing enterobacteriaceae of water samples in tunisia. | the presence of extended-spectrum beta-lactamase and plasmid-mediated ampc beta-lactamase producing enterobacteriaceae (esbl-eb and pampc-eb, respectively) was analyzed in 57 wastewater and 57 surface-water samples in tunisia. twenty-four of the 57 wastewater samples (42.1%) and one of the 57 surface-water samples (1.7%, a river that received effluents of a wastewater-treatment-plant) contained esbl-eb or pampc-eb; one esbl/pampc-eb per positive sample was further characterized. beta-lactamase g ... | 2016 | 26871556 |
| mechanisms of resistance to aminoglycoside antibiotics: overview and perspectives. | aminoglycoside (ag) antibiotics are used to treat many gram-negative and some gram-positive infections and, importantly, multidrug-resistant tuberculosis. among various bacterial species, resistance to ags arises through a variety of intrinsic and acquired mechanisms. the bacterial cell wall serves as a natural barrier for small molecules such as ags and may be further fortified via acquired mutations. efflux pumps work to expel ags from bacterial cells, and modifications here too may cause furt ... | 2015 | 26877861 |
| mechanisms of resistance to aminoglycoside antibiotics: overview and perspectives. | aminoglycoside (ag) antibiotics are used to treat many gram-negative and some gram-positive infections and, importantly, multidrug-resistant tuberculosis. among various bacterial species, resistance to ags arises through a variety of intrinsic and acquired mechanisms. the bacterial cell wall serves as a natural barrier for small molecules such as ags and may be further fortified via acquired mutations. efflux pumps work to expel ags from bacterial cells, and modifications here too may cause furt ... | 2015 | 26877861 |
| transient inhibition of ror-γt therapeutically limits intestinal inflammation by reducing th17 cells and preserving group 3 innate lymphoid cells. | rar-related orphan receptor-γt (ror-γt) directs differentiation of proinflammatory t helper 17 (th17) cells and is a potential therapeutic target in chronic autoimmune and inflammatory diseases. however, ror-γt-dependent group 3 innate lymphoid cells ilc3s provide essential immunity and tissue protection in the intestine, suggesting that targeting ror-γt could also result in impaired host defense after infection or enhanced tissue damage. here, we demonstrate that transient chemical inhibition o ... | 2016 | 26878233 |
| bacterial genomic epidemiology, from local outbreak characterization to species-history reconstruction. | bacteriology has embraced the next-generation sequencing revolution, swiftly moving from the time of single genome sequencing to the age of genomic epidemiology. hundreds and now even thousands of genomes are being sequenced for single bacterial species, allowing unprecedented levels of resolution and insight in the evolution and epidemic diffusion of the main bacterial pathogens. here, we present a review of some of the most recent and groundbreaking studies in this field. | 2015 | 26878934 |
| antibiotic resistance among urinary isolates from female outpatients in the united states in 2003 and 2012. | a retrospective analysis was performed using the surveillance network, usa, to examine the prevalence of antibiotic resistance among urine isolates from u.s. female outpatients in 2012 and assessed trends in antibiotic resistance comparing data from 2003 and 2012. the most common pathogen identified in 2012 (n = 285,325) was escherichia coli (64.9% of isolates). in 2012, e. coli resistance to nitrofurantoin was low (<3%) across all age groups. e. coli resistance to ciprofloxacin was high among a ... | 2016 | 26883714 |
| a highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species. | currently available tools for multiplex bacterial genome engineering are optimized for a few laboratory model strains, demand extensive prior modification of the host strain, and lead to the accumulation of numerous off-target modifications. building on prior development of multiplex automated genome engineering (mage), our work addresses these problems in a single framework. using a dominant-negative mutant protein of the methyl-directed mismatch repair (mmr) system, we achieved a transient sup ... | 2016 | 26884157 |
| restriction profiling of 23s microheterogenic ribosomal repeats for detection and characterizing of e. coli and their clonal, pathogenic, and phylogroups. | correlating ribosomal microheterogenicity with unique restriction profiles can prove to be an efficacious and cost-effective approach compared with sequencing for microbial identification. an attempt to peruse restriction profiling of 23s ribosomal assemblage was ventured; digestion patterns with bfa i discriminated e. coli from its colony morphovars, while hae iii profiles assisted in establishing distinct clonal groups. among the gene pool of 399 ribosomal sequences extrapolated from 57 e. col ... | 2015 | 26885397 |
| involvement of a novel class c beta-lactamase in the transglutaminase mediated cross-linking cascade of streptomyces mobaraensis dsm 40847. | streptomyces mobaraensis dsm 40847 secretes transglutaminase that cross-links proteins via γ-glutamyl-ε-lysine isopeptide bonds. characterized substrates are inhibitory proteins acting against various serine, cysteine and metalloproteases. in the present study, the bacterial secretome was examined to uncover additional transglutaminase substrates. fractional ethanol precipitation of the exported proteins at various times of culture growth, electrophoresis of the precipitated proteins, and sequen ... | 2016 | 26886195 |
| analysis for the presence of determinants involved in the transport of mercury across bacterial membrane from polluted water bodies of india. | mercury, which is ubiquitous and recalcitrant to biodegradation processes, threatens human health by escaping to the environment via various natural and anthropogenic activities. non-biodegradability of mercury pollutants has necessitated the development and implementation of economic alternatives with promising potential to remove metals from the environment. enhancement of microbial based remediation strategies through genetic engineering approaches provides one such alternative with a promisi ... | 2016 | 26887227 |
| complete genome sequence of multidrug-resistant citrobacter freundii strain p10159, isolated from urine samples from a patient with esophageal carcinoma. | citrobacter freundii is an opportunistic pathogen that can cause diarrhea, septicemia, meningitis, and urinary tract infections. we report here the complete genome sequence of c. freundii strain p10159, isolated from urine samples from a patient in china with esophageal carcinoma. the genome has 5,080,321 bp and 4,768 coding sequences, with a g+c content of 51.7%. | 2016 | 26893430 |
| enhancing phenotypic detection of esbl in ampc co-producers by using cefepime and tazobactam. | routine phenotypic methods employing clavulanate and third generation cephalosporins to detect esbl are not promising for isolates that co-produce an inhibitor-resistant beta lactamase like ampc. | 2016 | 26894064 |
| time for the 70°c water precautionary option in the home dilution of powdered infant formula. | powdered infant formulas (pif) are usually not sterile and may frequently be contaminated by several bacteria strains. among them, cronobacter species, previously known as enterobacter sakazakii, is one of the most harmful, since it might be the causative agent of sepsis and meningitis in newborns and preterm infants during the first weeks of life. the mortality rate of these infections is up to 80 %. therefore, some precautions are required in the home handling and dilution of pif. whereas ther ... | 2016 | 26895705 |
| biosurfactants produced by marine microorganisms with therapeutic applications. | marine microorganisms possess unique metabolic and physiological features and are an important source of new biomolecules, such as biosurfactants. some of these surface-active compounds synthesized by marine microorganisms exhibit antimicrobial, anti-adhesive and anti-biofilm activity against a broad spectrum of human pathogens (including multi-drug resistant pathogens), and could be used instead of existing drugs to treat infections caused by them. in other cases, these biosurfactants show anti ... | 2016 | 26901207 |
| nanosilver biocidal properties and their application in disinfection of hatchers in poultry processing plants. | the aim of this study was to use aqueous suspensions of silver nanoparticles with a wide spectrum of particle sizes, variable morphology, high stability, and appropriate physicochemical properties to examine their bactericidal and fungicidal properties against microorganisms present in poultry processing plants. at the same time, the particles were tested for preventing the production of odorogenous pollutants during incubation and thereby reducing the emission of harmful gases from such types o ... | 2016 | 26903785 |
| diagnostic methods of helicobacter pylori infection for epidemiological studies: critical importance of indirect test validation. | among the methods developed to detect h. pylori infection, determining the gold standard remains debatable, especially for epidemiological studies. due to the decreasing sensitivity of direct diagnostic tests (histopathology and/or immunohistochemistry [ihc], rapid urease test [rut], and culture), several indirect tests, including antibody-based tests (serology and urine test), urea breath test (ubt), and stool antigen test (sat) have been developed to diagnose h. pylori infection. among the ind ... | 2016 | 26904678 |
| rapid pcr detection of mycoplasma hominis, ureaplasma urealyticum, and ureaplasma parvum. | objective. we compared laboratory developed real-time pcr assays for detection of mycoplasma hominis and for detection and differentiation of ureaplasma urealyticum and parvum to culture using genitourinary specimens submitted for m. hominis and ureaplasma culture. methods. 283 genitourinary specimens received in the clinical bacteriology laboratory for m. hominis and ureaplasma species culture were evaluated. nucleic acids were extracted using the total nucleic acid kit on the magna pure 2.0. 5 ... | 2013 | 26904723 |
| characterization of a novel achromobacter xylosoxidans specific siphoviruse: phiaxp-1. | bacteriophages have recently been considered as an alternative biocontrol tool because of the widespread occurrence of antimicrobial-resistant achromobacter xylosoxidans. herein, we isolated a virulent bacteriophage (phiaxp-1) from a water sample of the bohai sea of china that specifically infects a. xylosoxidans. transmission electron microscopy revealed that phage phiaxp-1 belongs to the siphoviridae. we sequenced the genome of phiaxp-1, which comprises 45,045 bp with 64 open reading frames. m ... | 2016 | 26908262 |
| a novel 7-single nucleotide polymorphism-based clonotyping test allows rapid prediction of antimicrobial susceptibility of extraintestinal escherichia coli directly from urine specimens. | background. escherichia coli is a highly clonal pathogen. extraintestinal isolates belong to a limited number of genetically related groups, which often exhibit characteristic antimicrobial resistance profiles. methods. we developed a rapid clonotyping method for extraintestinal e coli based on detection of the presence or absence of 7 single nucleotide polymorphisms (snps) within 2 genes (fumc and fimh). a reference set of 2559 e coli isolates, primarily of urinary origin, was used to predict ... | 2016 | 26925427 |
| in vitro susceptibility to ceftazidime-avibactam of carbapenem-nonsusceptible enterobacteriaceae isolates collected during the inform global surveillance study (2012 to 2014). | the activity of ceftazidime-avibactam was assessed against 961 isolates of meropenem-nonsusceptible enterobacteriaceae most meropenem-nonsusceptible metallo-β-lactamase (mbl)-negative isolates (97.7%) were susceptible to ceftazidime-avibactam. isolates that carried kpc or oxa-48-like β-lactamases, both alone and in combination with extended-spectrum β-lactamases (esbls) and/or ampc β-lactamases, were 98.7% and 98.5% susceptible to ceftazidime-avibactam, respectively. meropenem-nonsusceptible, ca ... | 2016 | 26926648 |
| inflammatory diseases modelling in zebrafish. | the ingest of diets with high content of fats and carbohydrates, low or no physical exercise and a stressful routine are part of the everyday lifestyle of most people in the western world. these conditions are triggers for different diseases with complex interactions between the host genetics, the metabolism, the immune system and the microbiota, including inflammatory bowel diseases (ibd), obesity and diabetes. the incidence of these disorders is growing worldwide; therefore, new strategies for ... | 2016 | 26929916 |
| in vitro temocillin efficacy against extended spectrum β-lactamase producing multidrug resistant gram negative bacterial isolates from nepal. | temocillin is relatively more stable against most β-lactamases and requires re-evaluation to include it in common clinical practice as a therapeutic alternative. at the national reference laboratory of nepal, we evaluated multidrug resistance (mdr) and extended spectrum β-lactamase (esbl) phenotypes among 292 gram-negative clinical bacterial isolates of 18 different genera during 2009/2010 by kirby-bauer disc diffusion method following clsi guidelines. esbl screen positive isolates were tested f ... | 2014 | 26930733 |
| phenotypic detection and molecular characterization of beta-lactamase genes among citrobacter species in a tertiary care hospital. | to examine the distribution, emergence, and spread of genes encoding beta-lactamase resistance in citrobacter species isolated from hospitalized patients in a tertiary care hospital. | 2016 | 26952135 |
| new small plasmid harboring blakpc-2 in pseudomonas aeruginosa. | the aim of this study was to characterize the genetic context of blakpc-2 in pseudomonas aeruginosa sequence type 244 from brazil. the blakpc-2 gene was detected in a new small plasmid, pbh6. complete sequencing revealed that pbh6 was 3,652 bp long and included the tn3 resolvase and tn3 inverted repeat (ir), a partial copy of iskpn6, and a putative ori region but no rep genes. pbh6 replicated stably into escherichia coli strain dh10b and p. aeruginosa strain pao. | 2016 | 26953192 |
| key enzymes catalyzing glycerol to 1,3-propanediol. | biodiesel can replace petroleum diesel as it is produced from animal fats and vegetable oils, and it produces about 10 % (w/w) glycerol, which is a promising new industrial microbial carbon, as a major by-product. one of the most potential applications of glycerol is its biotransformation to high value chemicals such as 1,3-propanediol (1,3-pd), dihydroxyacetone (dha), succinic acid, etc., through microbial fermentation. glycerol dehydratase, 1,3-propanediol dehydrogenase (1,3-propanediol-oxydor ... | 2016 | 26966462 |
| employing bacterial microcompartment technology to engineer a shell-free enzyme-aggregate for enhanced 1,2-propanediol production in escherichia coli. | bacterial microcompartments (bmcs) enhance the breakdown of metabolites such as 1,2-propanediol (1,2-pd) to propionic acid. the encapsulation of proteins within the bmc is mediated by the presence of targeting sequences. in an attempt to redesign the pdu bmc into a 1,2-pd synthesising factory using glycerol as the starting material we added n-terminal targeting peptides to glycerol dehydrogenase, dihydroxyacetone kinase, methylglyoxal synthase and 1,2-propanediol oxidoreductase to allow their in ... | 2016 | 26969252 |
| nonproliferative and proliferative lesions of the gastrointestinal tract, pancreas and salivary glands of the rat and mouse. | the inhand (international harmonization of nomenclature and diagnostic criteria for lesions in rats and mice) project is a joint initiative of the societies of toxicologic pathology from europe (estp), great britain (bstp), japan (jstp), and north america (stp) to develop an internationally accepted nomenclature and diagnostic criteria for nonproliferative and proliferative lesions in laboratory animals. the purpose of this publication is to provide a standardized nomenclature and diagnostic cri ... | 2016 | 26973378 |
| gram-negative and -positive bacteria differentiation in blood culture samples by headspace volatile compound analysis. | identification of microorganisms in positive blood cultures still relies on standard techniques such as gram staining followed by culturing with definite microorganism identification. alternatively, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry or the analysis of headspace volatile compound (vc) composition produced by cultures can help to differentiate between microorganisms under experimental conditions. this study assessed the efficacy of volatile compound based ... | 2016 | 26973820 |
| trypanosoma cruzi differentiates and multiplies within chimeric parasitophorous vacuoles in macrophages coinfected with leishmania amazonensis. | the trypanosomatids leishmania amazonensis and trypanosoma cruzi are excellent models for the study of the cell biology of intracellular protozoan infections. after their uptake by mammalian cells, the parasitic protozoan flagellates l. amazonensis and t. cruzi lodge within acidified parasitophorous vacuoles (pvs). however, whereas l. amazonensis develops in spacious, phagolysosome-like pvs that may enclose numerous parasites, t. cruzi is transiently hosted within smaller vacuoles from which it ... | 2016 | 26975994 |
| characterization of an inca/c multidrug resistance plasmid in vibrio alginolyticus. | cephalosporin-resistant vibrio alginolyticus was first isolated from food products, with β-lactamases encoded by blaper-1, blaveb-1, and blacmy-2 being the major mechanisms mediating their cephalosporin resistance. the complete sequence of a multidrug resistance plasmid, pvas3-1, harboring the blacmy-2 and qnrvc4 genes was decoded in this study. its backbone exhibited genetic homology to known inca/c plasmids recoverable from members of the family enterobacteriaceae, suggesting its possible orig ... | 2016 | 26976864 |
| a universal culture medium for screening polymyxin-resistant gram-negative isolates. | the colistin-containing superpolymyxin medium was developed for screening polymyxin-resistant gram-negative bacteria. it was evaluated with 88 polymyxin-susceptible or polymyxin-resistant cultured gram-negative isolates. its sensitivity and specificity of detection were ca. 100%. the superpolymyxin medium is the first screening medium that is able to detect intrinsic and acquired polymyxin-resistant bacteria. | 2016 | 26984971 |
| evaluation of the septifast mgrade test on standard care wards--a cohort study. | the immediate need for appropriate antimicrobial therapy in septic patients requires the detection of the causative pathogen in a timely and reliable manner. in this study, the real-time pcr septifast mgrade test was evaluated in adult patients meeting the systemic inflammatory response syndrome (sirs) criteria that were treated at standard care wards. | 2016 | 26986826 |
| cofactor-embedded nanoporous activated carbon matrices for the immobilization of intracellular enzymes and degradation of endocrine disruptor. | the mixed intracellular enzyme (mice) from citrobacter freundii, capable of degrading o-phenylene diamine (opd), was extracted and characterized. cofactors such as zinc and copper ions enhanced the mice activity. the functionalized nanoporous-activated carbon (fnac) matrix, zinc-impregnated fnac matrix (zn(2+) -fnac), copper-impregnated fnac matrix (cu(2+) -fnac), and zinc- and copper-impregnated fnac matrix (zn(2+) -cu(2+) -fnac) were prepared and characterized to immobilize mice. the parameter ... | 2016 | 26988244 |
| hiding in fresh fruits and vegetables: opportunistic pathogens may cross geographical barriers. | different microbial groups of the microbiome of fresh produce can have diverse effects on human health. this study was aimed at identifying some microbial communities of fresh produce by analyzing 105 samples of imported fresh fruits and vegetables originated from different countries in the world including local samples (oman) for aerobic plate count and the counts of enterobacteriaceae, enterococcus, and staphylococcus aureus. the isolated bacteria were identified by molecular (pcr) and biochem ... | 2016 | 26989419 |
| drug and vaccine development for the treatment and prevention of urinary tract infections. | urinary tract infections (uti) are among the most common bacterial infections in humans, affecting millions of people every year. uti cause significant morbidity in women throughout their lifespan, in infant boys, in older men, in individuals with underlying urinary tract abnormalities, and in those that require long-term urethral catheterization, such as patients with spinal cord injuries or incapacitated individuals living in nursing homes. serious sequelae include frequent recurrences, pyelon ... | 2016 | 26999391 |
| antimicrobials from cnidarians. a new perspective for anti-infective therapy? | the ability of microbes to counter the scientific and therapeutic advancements achieved during the second half of the twentieth century to provide effective disease treatments is currently a significant challenge for researchers in biology and medicine. the discovery of antibiotics, and the subsequent development of synthetic antimicrobial compounds, altered our therapeutic approach towards infectious diseases, and improved the quality and length of life for humans and other organisms. the curre ... | 2016 | 27005633 |
| escherichia coli o104 in feedlot cattle feces: prevalence, isolation and characterization. | escherichia coli o104:h4, an hybrid pathotype of shiga toxigenic and enteroaggregative e. coli, involved in a major foodborne outbreak in germany in 2011, has not been detected in cattle feces. serogroup o104 with h type other than h4 has been reported to cause human illnesses, but their prevalence and characteristics in cattle have not been reported. our objectives were to determine the prevalence of e. coli o104 in feces of feedlot cattle, by culture and pcr detection methods, and characterize ... | 2016 | 27010226 |
| duct ectasia and periductal mastitis in indian women. | there is very little awareness of the general physicians and surgeons about the benign breast conditions such as duct ectasia (de) and periductal mastitis (pdm) causing nipple discharge. not only that these benign breast diseases ring a false alarm of cancer, they are also the second most common cause of benign breast diseases. the objective was to study the clinical and microbiological profiles of duct ectasia and periductal mastitis in indian women for better understanding of the disease proce ... | 2014 | 27011490 |
| duct ectasia and periductal mastitis in indian women. | there is very little awareness of the general physicians and surgeons about the benign breast conditions such as duct ectasia (de) and periductal mastitis (pdm) causing nipple discharge. not only that these benign breast diseases ring a false alarm of cancer, they are also the second most common cause of benign breast diseases. the objective was to study the clinical and microbiological profiles of duct ectasia and periductal mastitis in indian women for better understanding of the disease proce ... | 2014 | 27011490 |
| in vitro antimicrobial activity of traditional plant used in mestizo shamanism from the peruvian amazon in case of infectious diseases. | our survey was performed near iquitos (peruvian amazon) and its surroundings and leads us to consider mestizo ethnomedical practices. the plant species reported here are traditionally used for ailments related to microbial infections. inhabitants of various ethnic origins were interviewed, and 52 selected plants extracts were evaluated for their antimicrobial properties against a panel of 36 sensitive and multi-resistant bacteria or yeast. the study aimed at providing information on antimicrobia ... | 2015 | 27013805 |
| laboratory detection and clinical implication of oxacillinase-48 like carbapenemase: the hidden threat. | carbapenemase producing gram-negative pathogen is of great concern for physician. the challenging aspects are treatment option and infection control. monitoring of respective carbapenemase resistance mechanism is necessary to prevent the outbreaks. currently, the rapid emergence of oxacillinase (oxa-48) like is alarming. increasing frequency of oxa-48 is seen than the classical carbapenemase (kpc, ndm, imp, and vim) across the world. the bla oxa-48 gene is commonly identified in escherichia coli ... | 2016 | 27013843 |
| insights into newer antimicrobial agents against gram-negative bacteria. | currently, drug resistance, especially against cephalosporins and carbapenems, among gram-negative bacteria is an important challenge, which is further enhanced by the limited availability of drugs against these bugs. there are certain antibiotics (colistin, fosfomycin, temocillin, and rifampicin) that have been revived from the past to tackle the menace of superbugs, including members of enterobacteriaceae, acinetobacter species, and pseudomonas species. very few newer antibiotics have been add ... | 2016 | 27013887 |
| analysis of β-lactamase resistance determinants in enterobacteriaceae from chicago children: a multicenter survey. | multidrug-resistant (mdr) enterobacteriaceae infections are increasing in u.s. children; however, there is a paucity of multicentered analyses of antibiotic resistance genes responsible for mdr phenotypes among pediatric enterobacteriaceae isolates. in this study, 225 isolates phenotypically identified as extended-spectrum β-lactamase (esbl) or carbapenemase producers, recovered from children ages 0 to 18 years hospitalized between january 2011 and april 2015 at three chicago area hospitals, wer ... | 2016 | 27021322 |
| rapid detection of escherichia coli o157 and shiga toxins by lateral flow immunoassays. | shiga toxin-producing escherichia coli o157:h7 (stec) cause food-borne illness that may be fatal. stec strains enumerate two types of potent shiga toxins (stx1 and stx2) that are responsible for causing diseases. it is important to detect the e. coli o157 and shiga toxins in food to prevent outbreak of diseases. we describe the development of two multi-analyte antibody-based lateral flow immunoassays (lfia); one for the detection of stx1 and stx2 and one for the detection of e. coli o157 that ma ... | 2016 | 27023604 |