Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| campylobacter spp., giardia spp., cryptosporidium spp., noroviruses, and indicator organisms in surface water in southwestern finland, 2000-2001. | a total of 139 surface water samples from seven lakes and 15 rivers in southwestern finland were analyzed during five consecutive seasons from autumn 2000 to autumn 2001 for the presence of various enteropathogens (campylobacter spp., giardia spp., cryptosporidium spp., and noroviruses) and fecal indicators (thermotolerant coliforms, escherichia coli, clostridium perfringens, and f-rna bacteriophages) and for physicochemical parameters (turbidity and temperature); this was the first such systema ... | 2004 | 14711629 |
| detection of salmonella enterica serovar typhimurium by using a rapid, array-based immunosensor. | the multianalyte array biosensor (maab) is a rapid analysis instrument capable of detecting multiple analytes simultaneously. rapid (15-min), single-analyte sandwich immunoassays were developed for the detection of salmonella enterica serovar typhimurium, with a detection limit of 8 x 10(4) cfu/ml; the limit of detection was improved 10-fold by lengthening the assay protocol to 1 h. s. enterica serovar typhimurium was also detected in the following spiked foodstuffs, with minimal sample preparat ... | 2004 | 14711637 |
| multilocus sequence typing scheme for bacteria of the bacillus cereus group. | in this study we developed a multilocus sequence typing (mlst) scheme for bacteria of the bacillus cereus group. this group, which includes the species b. cereus, b. thuringiensis, b. weihenstephanensis, and b. anthracis, is known to be genetically very diverse. it is also very important because it comprises pathogenic organisms as well as bacteria with industrial applications. the mlst system was established by using 77 strains having various origins, including humans, animals, food, and soil. ... | 2004 | 14711642 |
| genomics of the cconoqp-encoded cbb3 oxidase complex in bacteria. | many bacteria adapt to microoxic conditions by synthesizing a particular cytochrome c oxidase (cbb3) complex with a high affinity for o2, encoded by the cconoqp operon. a survey of genome databases indicates that cconoqp sequences are widespread in all sub-branches of proteobacteria but otherwise are found only in bacteria of the cfb group ( cytophaga, flexibacter, bacteroides). our analysis of available genome sequences suggests four major strategies of regulating cconoqp expression in response ... | 2004 | 14714103 |
| fluorescent amplified fragment length polymorphism genotyping of campylobacter jejuni and campylobacter coli strains and its relationship with host specificity, serotyping, and phage typing. | fluorescent amplified fragment length polymorphism (faflp) analysis was applied to 276 campylobacter jejuni strains and 87 campylobacter coli strains isolated from humans, pigs, cattle, poultry, and retail meats to investigate whether certain faflp genotypes of c. jejuni and c. coli are associated with a particular host and to determine the degree of association between faflp-defined genotypes and heat-stable serotypes and/or phage types. within c. coli, the poultry strains clustered separately ... | 2004 | 14715757 |
| serotypes, virulence genes, and intimin types of shiga toxin (verotoxin)-producing escherichia coli isolates from human patients: prevalence in lugo, spain, from 1992 through 1999. | we have analyzed the prevalence of shiga toxin-producing escherichia coli (stec) in stool specimens of patients with diarrhea or other gastrointestinal alterations from the xeral-calde hospital of lugo city (spain). stec strains were detected in 126 (2.5%) of 5,054 cases investigated, with a progressive increase in the incidence from 0% in 1992 to 4.4% in 1999. stec o157:h7 was isolated in 24 cases (0.5%), whereas non-o157 stec strains were isolated from 87 patients (1.7%). stec strains were (af ... | 2004 | 14715771 |
| susceptibilities to different antibiotics of helicobacter pylori strains isolated from patients at the pediatric medical center of tehran, iran. | antibiotic susceptibility testing of 70 pediatric helicobacter pylori isolates was performed by using screening agar and disk diffusion methods. resistance to metronidazole and tinidazole was 72 to 79% and 71 to 81% by modified disk diffusion and 77% and 78% by screening agar, respectively. susceptibilities to amoxicillin, ampicillin, clarithromycin, tetracycline, erythromycin, and ciprofloxacin were 58, 69, 75, 68, 68, and 65%, respectively. | 2004 | 14715786 |
| use of genome selected repeated sequences increases the sensitivity of pcr detection of tropheryma whipplei. | the availability of the tropheryma whipplei genome offers the putative possibility of choosing logical dna targets. we applied a pcr assay (targeting repeated sequences of t. whipplei) to samples from patients with whipple's disease and to those from members of a control group. when compared to the results seen with regular pcr, the sensitivity of repeat pcr was significantly enhanced (p = 0.02) without alteration of its specificity. | 2004 | 14715790 |
| development of predictive models for the survival of campylobacter jejuni (atcc 43051) on cooked chicken breast patties and in broth as a function of temperature. | the objective of this study was to model the kinetics of the survival of campylobacter jejuni on cooked chicken breast patties and in broth as a function of temperature. both patties and broth were inoculated with 10(6) stationary-phase cells of a single strain of c. jejuni (atcc 43051) and incubated at constant temperatures from 4 to 30 degrees c in 2 degrees c increments under aerobic conditions. in most cases, a three-phase linear model fit the primary survival curves well (r2 = 0.97 to 0.99) ... | 2004 | 14717353 |
| fluoroquinolone susceptibility of campylobacter strains, senegal. | to assess fluoroquinolone susceptibility of campylobacter strains in senegal, skin samples were collected from 250 chicken carcasses from january 2001 to october 2002. among 205 isolated campylobacter strains, 59% and 41% were identified as campylobacter jejuni and c. coli, respectively; the overall ciprofloxacin-resistance rate was 34%. | 2003 | 14718098 |
| fluoroquinolone resistance in campylobacter absent from isolates, australia. | fluoroquinolone resistance was detected in 12 of 370 australian human campylobacter isolates; 10 of these were travel-associated, and for 2 isolates travel status was unknown. no resistance was found in isolates known to be locally acquired. in australia, fluoroquinolones have not been licensed for use in food production animals, a policy that may have relevance for countries with fluoroquinolone-resistant campylobacter. | 2003 | 14718099 |
| detection of campylobacter and shigella species in food samples using an array biosensor. | campylobacter and shigella bacteria are common causes of food- and water-borne illness worldwide. there is a current need in food, medical, environmental, and military markets for a rapid and user-friendly method of detecting such pathogens. the array biosensor developed at the nrl encompasses these qualities. in this study, 25-min, sandwich immunoassays were developed for the detection of campylobacter and shigella species in both buffer and a variety of food and beverage samples. the limit of ... | 2004 | 14719894 |
| possible role of autoantibodies in the pathophysiology of gm2 gangliosidoses. | mice containing a disruption of the hexb gene have provided a useful model system for the study of the human lysosomal storage disorder known as sandhoff disease (sd). hexb(-/-) mice rapidly develop a progressive neurologic disease of ganglioside gm2 and ga2 storage. our study revealed that the disease states in this model are associated with the appearance of antiganglioside autoantibodies. both elevation of serum antiganglioside autoantibodies and igg deposition to cns neurons were found in th ... | 2004 | 14722612 |
| [damage to peripheral nerves induced by campylobacter jejuni exotoxin]. | to explore the pathogenesis of the damage to peripheral nerves induced by campylobacter jejuni exotoxin (cjt). | 2003 | 14723820 |
| management of infectious diarrhoea. | 2004 | 14724167 | |
| immunoproliferative small intestinal disease associated with campylobacter jejuni. | immunoproliferative small intestinal disease (also known as alpha chain disease) is a form of lymphoma that arises in small intestinal mucosa-associated lymphoid tissue (malt) and is associated with the expression of a monotypic truncated immunoglobulin alpha heavy chain without an associated light chain. early-stage disease responds to antibiotics, suggesting a bacterial origin. we attempted to identify a causative agent. | 2004 | 14724303 |
| [effects of serum from a patient with acute motor axonal neuropathy on spinal motor neurons cultured in vitro]. | the effect of serum from a patient with acute motor axonal neuropathy (aman) on cultured motor neurons was studied. the ventral spinal ventral tissue was isolated from embryonic rats and digested into dissociated cell suspension for culture in vitro. the cultured cells were stained with smi-32, a non-phosphorylated neurofilment marker monoclonal antibody to identify motor neurons. the 6 days' cultured cells were exposed to the aman patient serum in a concentration of 25%, and to the normal human ... | 2003 | 14724936 |
| the streptococcus gordonii platelet binding protein gspb undergoes glycosylation independently of export. | the binding of bacteria and platelets may play a central role in the pathogenesis of infective endocarditis. platelet binding by streptococcus gordonii strain m99 is predominantly mediated by the 286-kda cell wall-anchored protein gspb. this unusually large protein lacks a typical amino-terminal signal peptide and is translocated from the cytoplasm via a dedicated transport system. a 14-kb segment just downstream of gspb encodes seca2 and secy2, two components of the gspb-specific transport syst ... | 2004 | 14729688 |
| the reca protein of helicobacter pylori requires a posttranslational modification for full activity. | the reca protein is a central component of the homologous recombination machinery and of the sos system in most bacteria. in performing these functions, it is involved in dna repair processes and plays an important role in natural transformation competence. this may be especially important in helicobacter pylori, where an unusually high degree of microdiversity among strains is generated by homologous recombination. we have suggested previously that the h. pylori reca protein is subject to postt ... | 2004 | 14729704 |
| mitochondrial proteins and complexes in leishmania and trypanosoma involved in u-insertion/deletion rna editing. | a number of mitochondrial proteins have been identified in leishmania sp. and trypanosoma brucei that may be involved in u-insertion/deletion rna editing. only a few of these have yet been characterized sufficiently to be able to assign functional names for the proteins in both species, and most have been denoted by a variety of species-specific and laboratory-specific operational names, leading to a terminology confusion both within and outside of this field. in this review, we summarize the pr ... | 2004 | 14730014 |
| structural analysis of the sialyltransferase cstii from campylobacter jejuni in complex with a substrate analog. | sialic acid terminates oligosaccharide chains on mammalian and microbial cell surfaces, playing critical roles in recognition and adherence. the enzymes that transfer the sialic acid moiety from cytidine-5'-monophospho-n-acetyl-neuraminic acid (cmp-neuac) to the terminal positions of these key glycoconjugates are known as sialyltransferases. despite their important biological roles, little is understood about the mechanism or molecular structure of these membrane-associated enzymes. we report th ... | 2004 | 14730352 |
| [experimental study on the therapeutic mechanism of high dose intravenous immunoglobulin in treatment of immune-mediated peripheral neuropathy]. | to explore the therapeutic basis of high dose intravenous immunoglobulin (ivig) in treatment of peripheral neuropathy induced by campylobacter jejuni lipopolysaccharide (cj lps). | 2003 | 14733812 |
| a survey of campylobacter species shed in faeces of beef cattle using polymerase chain reaction. | a polymerase chain reaction (pcr)-based survey of campylobacters associated with faeces collected from 382 beef cattle was undertaken. to ensure the removal of pcr inhibitors present in faeces and determine if adequate extraction was achieved, faeces were seeded with internal control dna (i.e., dna designed to amplify with the campylobacter genus primer set, but provide a smaller amplicon) before the extraction procedure. in only two samples (0.5%) were the internal control or campylobacter genu ... | 2003 | 14735214 |
| feob is not required for ferrous iron uptake in campylobacter jejuni. | among strains of campylobacter jejuni, levels of ferrous iron (fe2+) uptake was comparable. however, c. jejuni showed a lower level of ferrous iron uptake than escherichia coli. consistent with studies of e. coli, fe2+ uptake in c. jejuni was significantly enhanced by low mg2+ concentration. the c. jejuni genome sequence contains a single known ferrous iron uptake gene, feob, whose product shares 50% amino acid identity to helicobacter pylori feob and 29% identity to e. coli feob. however, fe2+ ... | 2003 | 14735223 |
| frequency of campylobacter jejuni in diarrhoea/dysentery in children in rawalpindi and islamabad. | to determine the frequency of campylobacter jejuni infection in children suffering from diarrhoea/dysentery in the department of microbiology, army medical college and military hospital, rawalpindi, from 29 august 2002 to 29 november 2002. | 2003 | 14738256 |
| patterns of variations in escherichia coli strains that produce cytolethal distending toxin. | a collection of 20 escherichia coli strains that produce cytolethal distending toxin (cdt) were analyzed for their virulence-associated genes. all of these strains were serotyped, and multiplex pcr analysis was used to ascertain the presence of genes encoding other virulence factors, including shiga toxin, intimin, enterohemolysin, cytotoxic necrotizing factor type 1 (cnf1) and cnf2, heat-stable toxin, and heat-labile toxin. these cdt-producing strains possessed various combinations of known vir ... | 2004 | 14742509 |
| two tonb systems in actinobacillus pleuropneumoniae: their roles in iron acquisition and virulence. | iron acquisition in vivo by actinobacillus pleuropneumoniae depends upon a functional tonb system. tonpitak et al. (w. tonpitak, s. thiede, w. oswald, n. baltes, and g.-f. gerlach, infect. immun. 68:1164-1170, 2000) have described one such system, associated with tbpba encoding the transferrin receptor, and here we report a second, termed tonb2. this gene cluster (exbb2-exbd2-tonb2) is highly homologous to those in other pasteurellaceae, unlike the earlier system described (now termed tonb1), su ... | 2004 | 14742511 |
| in vivo phase variation and serologic response to lipooligosaccharide of campylobacter jejuni in experimental human infection. | some campylobacter jejuni strains which exhibit mimicry of gangliosides in their lipooligosaccharides (loss) are associated with development of guillain-barré syndrome, which complicates the selection of a suitable c. jejuni strain in a live-attenuated vaccine. c. jejuni 81-176 is the most well characterized strain available, but structurally, los of c. jejuni 81-176 exhibits mimicry of predominantly gm2 and gm3 gangliosides. we compared the antiganglioside human serologic responses of 22 volunt ... | 2004 | 14742536 |
| the staphylococcal ferritins are differentially regulated in response to iron and manganese and via perr and fur. | staphylococcus aureus and staphylococcus epidermidis ferritin (ftna and sefa, respectively) homologues are antigenic and highly conserved. a previous study showed that ftna is a component of the s. aureus perr regulon with its transcription induced by elevated iron and repressed by perr, which functions as a manganese-dependent transcriptional repressor. we have further investigated the role of iron and fur in the regulation of perr regulon genes ftna (ferritin), ahpc (alkyl-hydroperoxidase), an ... | 2004 | 14742543 |
| gastroenteritis in nf-kappab-deficient mice is produced with wild-type camplyobacter jejuni but not with c. jejuni lacking cytolethal distending toxin despite persistent colonization with both strains. | campylobacter jejuni continues to be a leading cause of bacterial enteritis in humans. however, because there are no readily available animal models to study the pathogenesis of c. jejuni-related diseases, the significance of potential virulence factors, such as cytolethal distending toxin (cdt), in vivo are poorly understood. mice deficient in nf-kappab subunits (p50(-/-) p65(+/-)) in a c57bl/129 background are particularly susceptible to colitis induced by another enterohepatic microaerobe, he ... | 2004 | 14742559 |
| evidence for acquisition of the lipooligosaccharide biosynthesis locus in campylobacter jejuni gb11, a strain isolated from a patient with guillain-barré syndrome, by horizontal exchange. | campylobacter jejuni gb11, a strain isolated from a patient with guillain-barré syndrome, has been shown to be genetically closely related to the completely sequenced strain c. jejuni nctc 11168 by various molecular typing and serotyping methods. however, we observed that the lipooligosaccharide (los) biosynthesis genes strongly diverged between gb11 and nctc 11168. we sequenced the los biosynthesis locus of gb11 and found that it was nearly identical to the class a los locus from the c. jejuni ... | 2004 | 14742567 |
| comparison of campylobacter jejuni isolates implicated in guillain-barré syndrome and strains that cause enteritis by a dna microarray. | we asked whether campylobacter jejuni isolated from patients with guillain-barré syndrome (gbs) differ from isolates isolated from patients with uncomplicated gastrointestinal infection using dna microarray analysis. we found that specific gbs genes or regions were not identified, and microarray analysis confirmed significant genomic heterogeneity among the isolates. | 2004 | 14742576 |
| automated correction of genome sequence errors. | by using information from an assembly of a genome, a new program called autoeditor significantly improves base calling accuracy over that achieved by previous algorithms. this in turn improves the overall accuracy of genome sequences and facilitates the use of these sequences for polymorphism discovery. we describe the algorithm and its application in a large set of recent genome sequencing projects. the number of erroneous base calls in these projects was reduced by 80%. in an analysis of over ... | 2004 | 14744981 |
| selective detection and identification of sugar nucleotides by ce-electrospray-ms and its application to bacterial metabolomics. | a novel method employing ce-esms and precursor ion scanning was developed for the selective detection of nucleotide-activated sugars. by using precursor ion scanning for fragment ions specific to the different nucleotide carriers, i.e., ions at m/z 322 for cytidine monophosphate, m/z 323, 385, and 403 for uridine diphosphate, m/z 362, 424, and 442, for guanosine diphosphate, and m/z 346, 408, and 426 for adenosine diphosphate, it was possible to selectively detect sugar nucleotides involved in t ... | 2004 | 14750855 |
| emergence of variants with altered survival properties in stationary phase cultures of campylobacter jejuni. | during the stationary phase of campylobacter jejuni nctc 11351 viable numbers fluctuate in a characteristic fashion. after reaching the maximum cell count (ca. 2 x 10(9) cfu/ml) in early stationary phase (denoted phase 1), viable numbers subsequently decrease to about 10(6) cfu/ml after 48 h and then increase again to about 10(8) cfu/ml (denoted phase 2) before decreasing once more to a value intermediate between the previous maximum and minimum values. to investigate whether the increase in via ... | 2004 | 14751687 |
| differential expression of two paralogous genes of bacillus subtilis encoding single-stranded dna binding protein. | the bacillus subtilis genome comprises two paralogous single-stranded dna binding protein (ssb) genes, ssb and ywph, which show distinct expression patterns. the main ssb gene is strongly expressed during exponential growth and is coregulated with genes encoding the ribosomal proteins s6 and s18. the gene organization rpsf-ssb-rpsr as observed in b. subtilis is found in many gram-positive as well as some gram-negative bacteria, but not in escherichia coli. the ssb gene is essential for cell viab ... | 2004 | 14762004 |
| direct real-time pcr quantification of campylobacter jejuni in chicken fecal and cecal samples by integrated cell concentration and dna purification. | campylobacter jejuni is a major cause of diarrheal disease and food-borne gastroenteritis. the main reservoir of c. jejuni in poultry is the cecum, with an estimated content of 6 to 8 log10 cfu/g. if a flock is infected with c. jejuni, the majority of the birds in that flock will harbor the bacterium. diagnostics at the flock level could thus be an important control point. the aim of the work presented here was to develop a complete quantitative pcr-based detection assay for c. jejuni obtained d ... | 2004 | 14766556 |
| genotypic and antibiotic susceptibility characteristics of a campylobacter coli population isolated from dairy farmland in the united kingdom. | campylobacter infections are the most common cause of bacterial enteritis in humans, and nearly 8% of such infections are caused by campylobacter coli. most studies have concentrated on campylobacter jejuni, frequently isolated from intensively farmed poultry and livestock production units, and few studies have examined the spread and relatedness of campylobacter across a range of geographical and host boundaries. systematic sampling of a 100-km2 area of mixed farmland in northwest england yield ... | 2004 | 14766560 |
| comparison of survival of campylobacter jejuni in the phyllosphere with that in the rhizosphere of spinach and radish plants. | campylobacter jejuni has been isolated previously from market produce and has caused gastroenteritis outbreaks linked to produce. we have tested the ability of this human pathogen to utilize organic compounds that are present in leaf and root exudates and to survive in the plant environment under various conditions. carbon utilization profiles revealed that c. jejuni can utilize many organic acids and amino acids available on leaves and roots. despite the presence of suitable substrates in the p ... | 2004 | 14766604 |
| need for susceptibility testing guidelines for fastidious or less-frequently isolated bacteria. | 2004 | 14766806 | |
| comparison of mismatch amplification mutation assay with dna sequencing for characterization of fluoroquinolone resistance in neisseria gonorrhoeae. | a mismatch amplification mutation assay (mama) was developed for identification of point mutations in quinolone resistance-determining region (qrdr) of gyra at codons 91 and 95. mama pcr was used to detect mutations at codons 91 and 95 of gyra in 117 neisseria gonorrhoeae isolates (with ciprofloxacin mics of 0.004 to >32 microg/ml) from bangladesh during 1997 to 2001. the qrdr regions of the gyra genes from 31 randomly selected isolates were sequenced, and the results were compared with those of ... | 2004 | 14766821 |
| enzyme-linked immunosorbent assay for helicobacter pylori needs adjustment for the population investigated. | helicobacter pylori infection and peptic ulcer disease are common in developing countries, e.g., vietnam. an enzyme-linked immunosorbent assay (elisa) for screening of patients and for seroepidemiology is a useful tool but needs to be validated in the population studied. we used in-house elisa with sonicated swedish and vietnamese strains as antigens to measure immunoglobulin g antibodies after absorption with sonicated campylobacter jejuni in sera from 270 h. pylori culture-confirmed peptic ulc ... | 2004 | 14766827 |
| detection of species-specific helicobacter ribosomal dna in intestinal biopsy samples from a population-based cohort of patients with ulcerative colitis. | the inflammatory bowel diseases are considered an abnormal host immune response to an environmental stimulus. evidence suggests a role for intestinal bacteria in initiating and/or providing an ongoing stimulus for inflammation in inflammatory bowel disease. helicobacter pylori is the major cause of active chronic gastritis and peptic ulcers in humans and has been linked to gastric carcinoma and lymphoma. studies in various animal models, particularly mice, have identified enterohepatic helicobac ... | 2004 | 14766833 |
| genetic characterization and antibiotic resistance of campylobacter jejuni isolated from meats, water, and humans in sweden. | the incidence of campylobacter jejuni has increased during the last decade, and today it is the leading cause of bacterial enteritis in most developed countries. still, there is a lack of knowledge about infection routes and to what extent identified sources are responsible for spreading the bacterium to humans. the major objective of this work was to explore the genetic similarity between c. jejuni isolated from different sources. c. jejuni isolated from patients (n = 95), five types of meat (n ... | 2004 | 14766839 |
| simultaneous presence of multiple campylobacter species in dogs. | the prevalence of coinfection of campylobacter species in dogs was determined using four isolation methods. in 26% of the positive-testing stools, multiple campylobacter species were identified. the use of multiple isolation methods as well as the time lapse between sampling and processing are important for detection of coinfection. | 2004 | 14766860 |
| identification of bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of gram-stained material and discrimination from b. holmesii by using a unique reca gene restriction enzyme site. | bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial dna is amplified directly from sputum gram-stained slides. the validation of the method is described along with an additional new pcr-based assay that can distinguish between b. pertussis and bordetella holmesii. | 2004 | 14766870 |
| a methodology for environmental and occupational cancer surveys. | 1950 | 14834335 | |
| routes for campylobacter contamination of poultry meat: epidemiological study from hatchery to slaughterhouse. | from april 1998 to march 2000, 18 broiler flocks were followed from the hatchery to the slaughterhouse. campylobacter was not found in the hatchery, 1-day-old chicks or in the rearing house before the arrival of the chicks. the infection of broiler flocks increased continuously during the rearing time, with a total of seven positive flocks at the end of rearing. farms with campylobacter-positive broilers were characterized by the circulation of campylobacter in the environment (puddles, dung hil ... | 2003 | 14959785 |
| characterization of cj1293, a new udp-glcnac c6 dehydratase from campylobacter jejuni. | campylobacter jejuni encodes numerous sugar-nucleotide-modifying enzymes potentially involved in the biosynthesis of surface carbohydrates. one of them, cj1293, is involved in flagellin glycosylation but its biochemical activity remains unknown. using over-expressed and purified protein, we demonstrate that cj1293 has udp-glcnac-specific c(6) dehydratase activity. catalysis occurs without addition of cofactor, suggesting internal recycling of nad(p)(+). the k(m) for udp-glcnac of 50 microm indic ... | 2004 | 14960321 |
| the flgs/flgr two-component signal transduction system regulates the fla regulon in campylobacter jejuni. | the human pathogen campylobacter jejuni is a highly motile organism that carries a flagellum on each pole. the flagellar motility is regarded as an important trait in c. jejuni colonization of the intestinal tract, however, the knowledge of the regulation of this important colonization factor is rudimentary. we demonstrate by phosphorylation assays that the sensor flgs and the response regulator flgr form a two-component system that is on the top of the campylobacter flagellum hierarchy. phospho ... | 2004 | 14960570 |
| the genome sequence of bacillus cereus atcc 10987 reveals metabolic adaptations and a large plasmid related to bacillus anthracis pxo1. | we sequenced the complete genome of bacillus cereus atcc 10987, a non-lethal dairy isolate in the same genetic subgroup as bacillus anthracis. comparison of the chromosomes demonstrated that b.cereus atcc 10987 was more similar to b.anthracis ames than b.cereus atcc 14579, while containing a number of unique metabolic capabilities such as urease and xylose utilization and lacking the ability to utilize nitrate and nitrite. additionally, genetic mechanisms for variation of capsule carbohydrate an ... | 2004 | 14960714 |
| microbiological examination of ready-to-eat cold sliced meats and pâté from catering and retail premises in the uk. | to establish the microbiological quality of cold ready-to-eat sliced meats and pâté from catering and retail premises, and investigate links hypothesized between foodborne campylobacter infection and the consumption of cold sliced meats. | 2004 | 14962130 |
| design and diversity in bacterial chemotaxis: a comparative study in escherichia coli and bacillus subtilis. | comparable processes in different species often involve homologous genes. one question is whether the network structure, in particular the feedback control structure, is also conserved. the bacterial chemotaxis pathways in e. coli and b. subtilis both regulate the same task, namely, excitation and adaptation to environmental signals. both pathways employ many orthologous genes. yet how these orthologs contribute to network function in each organism is different. to investigate this problem, we p ... | 2004 | 14966542 |
| detection of pcr amplicons from bacterial pathogens using microsphere agglutination. | for rapid and inexpensive detection of polymerase chain reaction (pcr) amplicons, a novel microsphere agglutination assay has been developed. pcr is carried out using biotinylated forward and reverse primers, and the amplified dna fragments are able to agglutinate streptavidin-coated microspheres (5.7 microm in diameter). purification of pcr amplicons is unnecessary when initial primer concentrations are 250 nm. agglutination can be identified visually within 2 min without any additional equipme ... | 2004 | 14967231 |
| distribution and characterization of campylobacter spp. from russian poultry. | the distribution of campylobacter spp. on 13 poultry farms (broiler chicken, quail, pheasant, peacock, and turkey) from eight regions (vladimir, vologda, voronezh, kaluga, liptsk, moscow, orenburg, and orel) in russia was surveyed. intestinal materials were plated onto campylobacter-selective medium and plates were incubated microaerobically at 42 degrees c for 24 or 48 h. identification was based on colonial morphology, microscopic examination, and biochemical tests; latex agglutination assays ... | 2004 | 14968953 |
| prediction of rna-binding proteins from primary sequence by a support vector machine approach. | elucidation of the interaction of proteins with different molecules is of significance in the understanding of cellular processes. computational methods have been developed for the prediction of protein-protein interactions. but insufficient attention has been paid to the prediction of protein-rna interactions, which play central roles in regulating gene expression and certain rna-mediated enzymatic processes. this work explored the use of a machine learning method, support vector machines (svm) ... | 2004 | 14970381 |
| consumption of foods by young children with diagnosed campylobacter infection - a pilot case-control study. | to determine whether parentally reported habitual intake of specific foods differed between children with diagnosed campylobacter jejuni infection and children of a comparison group without diagnosed infection. | 2004 | 14972076 |
| eburst: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. | the introduction of multilocus sequence typing (mlst) for the precise characterization of isolates of bacterial pathogens has had a marked impact on both routine epidemiological surveillance and microbial population biology. in both fields, a key prerequisite for exploiting this resource is the ability to discern the relatedness and patterns of evolutionary descent among isolates with similar genotypes. traditional clustering techniques, such as dendrograms, provide a very poor representation of ... | 2004 | 14973027 |
| differential impact of mexb mutations on substrate selectivity of the mexab-oprm multidrug efflux pump of pseudomonas aeruginosa. | the integral inner membrane resistance-nodulation-division (rnd) components of three-component rnd-membrane fusion protein-outer membrane factor multidrug efflux systems define the substrate selectivity of these efflux systems. to gain a better understanding of what regions of these proteins are important for substrate recognition, a plasmid-borne mexb gene encoding the rnd component of the mexab-oprm multidrug efflux system of pseudomonas aeruginosa was mutagenized in vitro by using hydroxylami ... | 2004 | 14973037 |
| die-off of enteric bacterial pathogens during mesophilic anaerobic digestion. | conventionally treated sewage sludge may contain high concentrations of potentially pathogenic microorganisms and additional treatment is required to minimise the risks to health if it is to be recycled to agricultural land. mesophilic anaerobic digestion (mad) is the most widely used process in the uk for stabilising sludge prior to agricultural recycling, but little is known about the fate of a number of enteric pathogens as the sludge passes through the treatment processes. the aim of this st ... | 2004 | 14975643 |
| optimization of pcr-based methods for rapid detection of campylobacter jejuni, campylobacter coli and yersinia enterocolitica serovar 0:3 in wastewater samples. | pcr-based methods were evaluated for their adequacy to assess the removal of pathogens from wastewater samples. for the development and optimization of the methods, samples were taken at two different sites from two different constructed wetlands. campylobacter jejuni/coli and yersinia enterocolitica serogroup 0:3 were selected as model pathogens and enterococcus faecalis as a standard microbiological indicator. the chosen pcr protocols were optimized for wastewater dna extracts in order to obta ... | 2004 | 14975667 |
| an nadph quinone reductase of helicobacter pylori plays an important role in oxidative stress resistance and host colonization. | oxidative stress resistance is one of the key properties that enable pathogenic bacteria to survive the toxic reactive oxygen species released by the host. in a previous study characterizing oxidative stress resistance mutants of helicobacter pylori, a novel potential antioxidant protein (mdab) was identified by the observation that the expression of this protein was significantly upregulated to compensate for the loss of other major antioxidant components. in this study, we characterized an h. ... | 2004 | 14977943 |
| cross-species surface display of functional spirochetal lipoproteins by recombinant borrelia burgdorferi. | surface-exposed lipoproteins of relapsing fever (rf) and lyme borreliosis borrelia spirochetes mediate certain interactions of the bacteria with their arthropod and vertebrate hosts. rf spirochetes such as borrelia hermsii serially evade the host's antibody response by multiphasic antigenic variation of vsp and vlp proteins. furthermore, the expression of vsp1 and vsp2 by borrelia turicatae is associated with neurotropism and higher blood densities, respectively. in contrast to rf borrelia speci ... | 2004 | 14977951 |
| tetracycline and streptomycin resistance genes, transposons, and plasmids in salmonella enterica isolates from animals in italy. | fifty-eight multidrug-resistant salmonella enterica strains of 20 serotypes, isolated from animal sources in italy, were analyzed for tet(a) and stra-strb, conferring tetracycline and streptomycin resistance, respectively. the stra and strb genes were highly prevalent in salmonella strains of our collection, being detected in 84% of the streptomycin-resistant strains. in many strains, the stra and strb genes were linked to a particular tn5393-derivative transposon characterized by the presence o ... | 2004 | 14982782 |
| genome-wide expression analyses of campylobacter jejuni nctc11168 reveals coordinate regulation of motility and virulence by flha. | we examined two variants of the genome-sequenced strain, campylobacter jejuni nctc11168, which show marked differences in their virulence properties including colonization of poultry, invasion of caco-2 cells, and motility. transcript profiles obtained from whole genome dna microarrays and proteome analyses demonstrated that these differences are reflected in late flagellar structural components and in virulence factors including those involved in flagellar glycosylation and cytolethal distendin ... | 2004 | 14985343 |
| a bipolar dna helicase gene, hera, clusters with rad50, mre11 and nura genes in thermophilic archaea. | we showed previously that rad50 and mre11 genes of thermophilic archaea are organized in an operon-like structure with a third gene (nura) encoding a 5' to 3' exonuclease. here, we show that the rad50, mre11 and nura genes from the hyperthermophilic archaeon sulfolobus acidocaldarius are co-transcribed with a fourth gene encoding a dna helicase. this enzyme (hera) is the prototype of a new class of dna helicases able to utilize either 3' or 5' single-stranded dna extensions for loading and subse ... | 2004 | 14990749 |
| comparative analysis of protein domain organization. | we have developed a set of graph theory-based tools, which we call comparative analysis of protein domain organization (cado), to survey and compare protein domain organizations of different organisms. in the language of cado, the organization of protein domains in a given organism is shown as a domain graph in which protein domains are represented as vertices, and domain combinations, defined as instances of two domains found in one protein, are represented as edges. cado provides a new way to ... | 2004 | 14993202 |
| campylobacter jejuni infection of differentiated thp-1 macrophages results in interleukin 1 beta release and caspase-1-independent apoptosis. | apoptosis induction of host macrophages has emerged as a common virulence mechanism among bacterial pathogens. infection with campylobacter jejuni is a leading cause of gastroenteritis worldwide and is characterized by an acute inflammatory response in the small intestine. the authors used the human monocytic cell line thp-1 to examine apoptosis induction and pro-inflammatory cytokine production during c. jejuni infection. flow cytometric analysis revealed that 48 h after inoculation, a c. jejun ... | 2004 | 14993305 |
| reactive arthritis following an outbreak of campylobacter jejuni infection. | to study the occurrence and the clinical picture of musculoskeletal (msk) complications including reactive arthritis (rea) following an outbreak of campylobacter jejuni. | 2004 | 14994400 |
| campylobacter spp. in irish feedlot cattle: a longitudinal study involving pre-harvest and harvest phases of the food chain. | the aim of this study was to investigate faecal shedding and transmission of campylobacter spp. in cohorts of cattle within a feedlot, to assess subsequent contamination of carcasses with this pathogen and to identify risk factors associated with faecal shedding of campylobacter spp. a cohort of 133 heifers housed in four adjacent pens was examined over a five and a half month period, from entering the feedlot to slaughter. a parallel investigation of individual rectal faecal samples and pen env ... | 2004 | 14995974 |
| phosphatidylethanolamine is not essential for growth of sinorhizobium meliloti on complex culture media. | in addition to phosphatidylglycerol (pg), cardiolipin (cl), and phosphatidylethanolamine (pe), sinorhizobium meliloti also possesses phosphatidylcholine (pc) as a major membrane lipid. the biosynthesis of pc in s. meliloti can occur via two different routes, either via the phospholipid n-methylation pathway, in which pe is methylated three times in order to obtain pc, or via the phosphatidylcholine synthase (pcs) pathway, in which choline is condensed with cdp-diacylglycerol to obtain pc directl ... | 2004 | 14996797 |
| induction of alpha and beta chemokines by intestinal epithelial cells stimulated with campylobacter jejuni. | to investigate the production of dynamic alpha and beta chemokines represented by interleukin-8 (il-8) as alpha chemokine and ccl2 (monocyte-chemoattractant protein-1, ccr2 ligand), ccl4 (macrophage-inflammatory protein-1beta, ccr5 ligand), ccl3 (macrophage-inflammatory protein-1alpha, ccr1/5 ligand), (ccl5, regulated upon activation, normal t-cell expressed and secreted (rantes, ccr5 ligand) as beta chemokines by the human intestinal cell line int407 stimulated with factors produced by living c ... | 2004 | 15001302 |
| visualization of the phylogenetic content of five genomes using dekapentagonal maps. | the methods presented here summarize phylogenetic relationships of genomes in visually appealing and informative figures. dekapentagonal maps depict phylogenetic information for orthologous genes present in five genomes, and provide a pre-screen for putatively horizontally transferred genes. if the majority of individual gene phylogenies are unresolved, bipartition histograms provide a means of uncovering and analyzing the plurality consensus. analyses of genomes representing five photosynthetic ... | 2004 | 15003123 |
| multiplex pcr assay for differentiation of helicobacter felis, h. bizzozeronii, and h. salomonis. | helicobacter felis, helicobacter bizzozeronii, and helicobacter salomonis are frequently found in the gastric mucous membrane of dogs and cats. these large spiral organisms are phylogenetically highly related to each other. their fastidious nature makes it difficult to cultivate them in vitro, hampering traditional identification methods. we describe here a multiplex pcr test based on the trna intergenic spacers and on the urease gene, combined with capillary electrophoresis, that allows discrim ... | 2004 | 15004062 |
| simultaneous detection of entamoeba histolytica, giardia lamblia, and cryptosporidium parvum in fecal samples by using multiplex real-time pcr. | entamoeba histolytica, giardia lamblia, and cryptosporidium are three of the most important diarrhea-causing parasitic protozoa. for many years, microscopic examination of stool samples has been considered to be the "gold standard" for diagnosis of e. histolytica, g. lamblia, and c. parvum infections. recently, more specific and sensitive alternative methods (pcr, enzyme-linked immunosorbent assay, and direct fluorescent-antibody assay) have been introduced for all three of these parasitic infec ... | 2004 | 15004079 |
| occurrence of campylobacter jejuni in pets living with human patients infected with c. jejuni. | campylobacter jejuni was recovered from four dogs (11%) and four cats (33%) living with danish human patients infected with c. jejuni. pulsed-field gel electrophoresis (pfge) analysis revealed the occurrence of the same quinolone-resistant strain in a girl and her dog. c. jejuni isolates with closely related (>95% similarity) pfge profiles occurred in humans and pets from different danish counties. | 2004 | 15004120 |
| [campylobacteriosis in a low-income community in buenos aires, argentina]. | the authors describe the problem of campylobacteriosis in a low-income community located in southern buenos aires. homes in the area were classified according to their total number of inhabitants, the number of children and animals living in the house and the amount of poultry meat consumed. samples were taken from all the different types of homes that had been identified in order to isolate, identify and type campylobacter sp., using the modified skirrow technique, bio-chemical tests and the li ... | 2003 | 15005557 |
| vanadium(v) reduction by shewanella oneidensis mr-1 requires menaquinone and cytochromes from the cytoplasmic and outer membranes. | the metal-reducing bacterium shewanella oneidensis mr-1 displays remarkable anaerobic respiratory plasticity, which is reflected in the extensive number of electron transport components encoded in its genome. in these studies, several cell components required for the reduction of vanadium(v) were determined. v(v) reduction is mediated by an electron transport chain which includes cytoplasmic membrane components (menaquinone and the tetraheme cytochrome cyma) and the outer membrane (om) cytochrom ... | 2004 | 15006760 |
| diversity of microbial sialic acid metabolism. | sialic acids are structurally unique nine-carbon keto sugars occupying the interface between the host and commensal or pathogenic microorganisms. an important function of host sialic acid is to regulate innate immunity, and microbes have evolved various strategies for subverting this process by decorating their surfaces with sialylated oligosaccharides that mimic those of the host. these subversive strategies include a de novo synthetic pathway and at least two truncated pathways that depend on ... | 2004 | 15007099 |
| acute facial diplegia and hyperreflexia: a guillain-barré syndrome variant. | two patients with acute facial diplegia and hyperreflexia are described. both patients had serologic evidence of preceding campylobacter jejuni infection and antiganglioside igg antibodies as well as other laboratory and electrophysiologic findings suggesting guillain-barré syndrome (gbs). iv immunoglobulin produced recovery. hyperreflexia does not necessarily exclude the diagnosis of a gbs variant. antiganglioside antibodies can help with diagnosis in difficult cases. | 2004 | 15007144 |
| genetic relatedness among campylobacter jejuni serotyped isolates of diverse origin as determined by numerical analysis of amplified fragment length polymorphism (aflp) profiles. | to use amplified fragment length polymorphism (aflp) analysis to evaluate the genetic relatedness among 254 campylobacter jejuni reference and field strains of diverse origin representing all defined 'penner' serotypes for this species. | 2004 | 15012818 |
| diversities and similarities in pfge profiles of campylobacter jejuni isolated from migrating birds and humans. | to genetically sub-type campylobacter jejuni strains isolated from migratory birds, and to compare these with clinical strains collected in the same area and corresponding time period, with the aim to increase our knowledge on sub-types occurring among wild birds and their possible impact on human disease. | 2004 | 15012823 |
| lymphocytic colitis: a retrospective clinical study of 199 swedish patients. | lymphocytic colitis is characterised by chronic diarrhoea and specific microscopic changes in a macroscopically normal colonic mucosa. we report clinical features and treatment outcome in a large patient cohort. | 2004 | 15016748 |
| guillain-barré syndrome: epidemiology, pathophysiology and management. | guillain-barré syndrome (gbs) is clinically defined as an acute peripheral neuropathy causing limb weakness that progresses over a time period of days or, at the most, up to 4 weeks. gbs occurs throughout the world with a median annual incidence of 1.3 cases per population of 100 000, with men being more frequently affected than women. gbs is considered to be an autoimmune disease triggered by a preceding bacterial or viral infection. campylobacter jejuni, cytomegalovirus, epstein-barr virus and ... | 2004 | 15018590 |
| haemophilus influenzae as a possible cause of guillain-barré syndrome. | recent reports have contained conflicting results on the relationship between antecedent haemophilus influenzae infection and guillain-barré syndrome (gbs). to investigate the prevalence of h. influenzae infection in gbs patients in a british population, we carried out a retrospective study with 62 consecutive gbs patients and 63 normal controls of similar age and sex. whole bacteria of both encapsulated and nonencapsulated strains of h. influenzae were employed as antigens in an enzyme-linked i ... | 2004 | 15020076 |
| [the role of certain campylobacter types in the etiology of enterocolitis]. | in recent decades, medical community has increasingly been calling attention to the importance of campylobacter as an disease-causing agent in humans. nowadays, campylobacter jejuni (c. jejuni) is known as the most frequent bacterial cause of diarrhea worldwide. epidemiological differences of the infections caused by campylobacter, present in the developed and the developing countries, are attributed to the differences of the types of virulence. due to the specificity, and the demanding features ... | 2004 | 15022385 |
| involvement of capsaicin-sensitive afferent nerves and cholecystokinin 2/gastrin receptors in gastroprotection and adaptation of gastric mucosa to helicobacter pylori-lipopolysaccharide. | lipopolysaccharide (lps) is one of the virulence factors in the helicobacter pylori (hp)-infected stomach, but it remains unknown whether single and prolonged pretreatment with hp-lps can affect the course of gastric damage induced by aspirin (asa). we compared the effects of hp-lps with those induced by lpss isolated from intestinal bacteroides fragilis, yersinia enterocolitica, and campylobacter jejuni applied for 4 days on acute asa-induced gastric lesions in rats. the area of asa-induced gas ... | 2004 | 15024038 |
| an outbreak of gastroenteritis from a non-chlorinated community water supply. | to determine the source and the extent of a community wide outbreak of gastroenteritis. | 2004 | 15026434 |
| identification of bacillus anthracis specific chromosomal sequences by suppressive subtractive hybridization. | bacillus anthracis, bacillus thuringiensis and bacillus cereus are closely related members of the b. cereus-group of bacilli. suppressive subtractive hybridization (ssh) was used to identify specific chromosomal sequences unique to b. anthracis. | 2004 | 15028116 |
| sequence polymorphism in the glycosylation island and flagellins of pseudomonas aeruginosa. | a genomic island consisting of 14 open reading frames, orfa to orfn was previously identified in pseudomonas aeruginosa strain pak and shown to be essential for glycosylation of flagellin. dna microarray hybridization analysis of a number of p. aeruginosa strains from diverse origins showed that this island is polymorphic. pcr and sequence analysis confirmed that many p. aeruginosa strains carry an abbreviated version of the island (short island) in which orfd, -e and -h are polymorphic and orfi ... | 2004 | 15028697 |
| acquisition of quinolone resistance and point mutation of the gyra gene in campylobacter jejuni isolated from broilers and in vitro-induced resistant strains. | a dramatic rise in the number of resistant campylobacter to quinolones has been documented in human patients and domestic animals. in this study, the mechanism of acquisition of quinolone resistance was studied by detecting point mutations in the gyra gene of campylobacter strains obtained from broilers and strains with in vitro-induced resistance. the minimal inhibitory concentrations (mics) of norfloxacin (nflx) and ofloxacin (oflx) for the strains that had no point mutation were slightly incr ... | 2004 | 15031543 |
| prevalence and characteristics of shiga toxin-producing escherichia coli, salmonella spp. and campylobacter spp. isolated from slaughtered sheep in switzerland. | caecum samples collected from 653 slaughtered sheep from two swiss abattoirs were examined. the aim of this study was: (i) to determine the prevalence of shiga toxin-producing escherichia coli (stec), salmonella spp. and campylobacter spp.; (ii) to further characterize isolated strains; and (iii) to discuss the results obtained with their relevance to food safety. the percentage of samples testing positive for stec by a polymerase chain reaction was 29.9%. the prevalence of positive salmonella s ... | 2004 | 15033267 |
| the versatile bacterial type iv secretion systems. | bacteria use type iv secretion systems for two fundamental objectives related to pathogenesis--genetic exchange and the delivery of effector molecules to eukaryotic target cells. whereas gene acquisition is an important adaptive mechanism that enables pathogens to cope with a changing environment during invasion of the host, interactions between effector and host molecules can suppress defence mechanisms, facilitate intracellular growth and even induce the synthesis of nutrients that are benefic ... | 2003 | 15035043 |
| prevalence of helicobacter pylori infection among adult dyspeptic patients in ethiopia. | in developing countries such as ethiopia, where chronic gastritis and peptic-ulcer disease are the most common endoscopic findings, it is important to study the association between helicobacter pylori infection and gastroduodenal diseases. both invasive and non-invasive diagnostic methods were therefore used to investigate 300, consecutive, adult patients with dyspepsia, from the gastrointestinal clinic of tikur anbassa university hospital, addis ababa. the apparent overall prevalence of h. pylo ... | 2004 | 15035728 |
| normally occurring human anti-gm1 immunoglobulin m antibodies and the immune response to bacteria. | anti-gm(1) antibodies of the immunoglobulin m (igm) isotype are normal components of the antibody repertoire of adult human serum. using a sensitive high-performance thin-layer chromatography (hptlc) immunostaining assay, we found that these antibodies were absent in the umbilical vein and children <1 month of age but could be detected after 1 month of age. although most of the children older than 6 months of age were positive, there were still a few negative children. the appearance of anti-gm( ... | 2004 | 15039337 |
| a deep-rough mutant of campylobacter jejuni 81-176 is noninvasive for intestinal epithelial cells. | a waaf mutant of campylobacter jejuni 81-176 showed decreased invasion of int407 cells in vitro and increased sensitivity to some antibiotics compared to what was seen with the wild-type strain. | 2004 | 15039381 |
| the iron-sulfur cluster assembly genes iscs and iscu of entamoeba histolytica were acquired by horizontal gene transfer. | iron-sulfur (fes) proteins are present in all living organisms and play important roles in electron transport and metalloenzyme catalysis. the maturation of fes proteins in eukaryotes is an essential function of mitochondria, but little is known about this process in amitochondriate eukaryotes. here we report on the identification and analysis of two genes encoding critical fes cluster (isc) biosynthetic proteins from the amitochondriate human pathogen entamoeba histolytica. | 2004 | 15040816 |
| molecular evolution of hisb genes. | the sixth and eighth steps of histidine biosynthesis are catalyzed by an imidazole glycerol-phosphate (igp) dehydratase (ec 4.2.1.19) and by a histidinol-phosphate (hol-p) phosphatase (ec 3.1.3.15), respectively. in the enterobacteria, in campylobacter jejuni and in xylella/xanthomonas the two activities are associated with a single bifunctional polypeptide encoded by hisb. on the other hand, in archaea, eucarya, and most bacteria the two activities are encoded by two separate genes. in this wor ... | 2004 | 15042344 |
| regulatory regions and critical residues of nod2 involved in muramyl dipeptide recognition. | multiple genetic variants of card15/nod2 have been associated with susceptibility to crohn's disease and blau syndrome. nod2 recognizes muramyl dipeptide (mdp) derived from bacterial peptidoglycan (pgn), but the molecular basis of recognition remains elusive. we performed systematic mutational analysis to gain insights into the function of nod2 and molecular mechanisms of disease susceptibility. using an archive of 519 mutations covering approximately 50% of the amino-acid residues of nod2, the ... | 2004 | 15044951 |
| resistotyping of campylobacter jejuni. | the principal objective of typing in epidemiology is to trace a strain as it passes from one individual to another. resistotyping is a phenotypic method that consists of testing bacterial strains against a set of arbitrarily chosen chemical agents, whereby, a resistance pattern that is characteristic of a strain is generated and, is believed to describe the isolates for epidemiological purposes. this simple typing system is described for campylobacter isolated in lagos, nigeria. resistotyping wa ... | 2003 | 15045011 |