Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| viruses and bone marrow failure. | some generalizations can be drawn from a review of virus-associated bone marrow failure. the story of b19 parvovirus illustrates that viral infection may be an occult cause of marrow failure. although the epidemiology of transient aplastic crisis suggested a viral aetiology, the implication of a single virus was surprising; the sporadic appearance of chronic bone marrow failure in immunosuppressed persons has had none of the features of a viral illness. the incrimination of parvovirus in these c ... | 1989 | 2537667 |
| persistent productive hiv infection in ebv-transformed b lymphocytes. | the susceptibility to hiv infection of 14 b-cell lines established from five healthy hiv seronegative and from six hiv seropositive subjects by lymphocyte transformation with ebv and/or by lymphocyte cultivation with cyclosporin a was studied. although the cell lines contained different proportions of cd4-positive cells, as shown by flow cytometry, all of them could be infected with the sf-2 strain of hiv. infection was blocked by a monoclonal antibody directed against the viral attachment site ... | 1989 | 2537875 |
| vero cell-expressed epstein-barr virus (ebv) gp350/220 protects marmosets from ebv challenge. | the epstein-barr virus (ebv) major membrane antigen, gp350/220, was purified from expressing, genetically engineered vero cells. the antigen, formulated either with alum or freund's adjuvant, was inoculated into ebv infection-susceptible marmosets. after several injections, most of the marmosets developed anti-gp350/220 antibodies, and several exhibited virus-neutralizing activity. the immune response elicited by the alum-absorbed antigen proved to be protective upon virus challenge of the inocu ... | 1989 | 2537882 |
| abnormal glucocorticoid receptor gene and mrna in primary cortisol resistance. | abnormal steroid hormone receptors have been implicated as causing several forms of primary steroid hormone resistance in humans, but as yet no abnormality has been described at the gene level. we describe the analysis of the mrna and genomic dna from the epstein-barr (eb) virus transformed cells of two siblings with primary cortisol resistance. the cells of the propositus and his brother show a decreased level of glucocorticoid receptor (gr) mrna, and the genomic dna of both individuals shows a ... | 1989 | 2537912 |
| hodgkin's disease and epstein-barr virus. altered antibody pattern before diagnosis. | in patients with hodgkin's disease, titers of igg antibody against viral capsid antigen of epstein-barr virus and the prevalence of antibodies against early antigen are higher than expected. to evaluate whether this condition antedates diagnosis, we identified 43 persons with hodgkin's disease, from whom blood had been drawn and stored for an average of 50.5 months before diagnosis, and 96 controls from the same populations, from whom blood had been drawn at the same time. the relative risks of ... | 1989 | 2537928 |
| epstein-barr virus-related lymphoproliferative disorders following bone marrow transplantation: an immunologic and genotypic analysis. | four patients from 1.5 to 18 yr of age who had received partially matched t-cell-depleted bone marrow transplants for acute leukemia succumbed to a widespread lymphoproliferative disorder (lpd) at 56 to 147 days after transplant. premortem diagnosis of lpd was suggested in two because plasmacytoid cells were observed in the blood and bone marrow, and in the cerebrospinal fluid of one of these patients. serum clonal immunoglobulins (igs) were also demonstrated in these two patients premortem, whi ... | 1989 | 2537974 |
| how dna viruses may cause cancer. | 1989 | 2538000 | |
| production of human warm-reacting red cell monoclonal autoantibodies by epstein-barr virus transformation. | monoclonal antibody technology has been used in both murine and human systems to produce a variety of antibodies that react with the human red cell (rbc). rbc monoclonal autoantibodies have been obtained from animal models of autoimmune hemolytic anemia (aiha), but to date no warm-reactive monoclonal autoantibodies have been generated from human b cells. using the epstein-barr virus (ebv) transformation method, clones of rbc autoantibodies were generated from two patients with aiha. these antibo ... | 1989 | 2538013 |
| confirmation of the heterogeneity of posttransplant epstein-barr virus-associated b cell proliferations by immunoglobulin gene rearrangement analyses. | immunoglobulin gene rearrangement analysis is a sensitive method for determining clonality of b cell proliferations. we have examined tissue obtained from five renal and one cardiac allograft recipient with epstein-barr virus-associated b cell proliferations for immunoglobulin gene rearrangements. biopsies from two patients with lesions that were hyperplastic morphologically, polyclonal by cellular immunoglobulin staining, and had diploid karyotypes, had no detectable gene rearrangements and wer ... | 1989 | 2538015 |
| epstein-barr virus infections following okt3 treatment. | 1989 | 2538016 | |
| in situ demonstration of epstein-barr viral genomes in viral-associated b cell lymphoproliferations. | thirteen cases of benign and malignant epstein-barr viral (ebv)-associated b cell lymphoproliferations were examined by in situ hybridization studies performed on formalin-fixed, paraffin-embedded tissue sections. ebv nucleic acids were identified in a minority of lymphoid cells in five of six cases of benign infectious mononucleosis studied in tonsil or lymph node specimens. no evidence of ebv was found in two splenectomy specimens from patients with infectious mononucleosis. ebv nucleic acids ... | 1989 | 2538060 |
| relationship between methylation status and expression of an epstein-barr virus (ebv) capsid antigen gene. | the methylation status of the 160 kd viral capsid antigen (vca) gene promoter was determined by hybridization analysis. the semi-permissive marmoset cell line ff41-1 lacked cytosine methylation in approximately three quarters of the vca promoter cpg dinucleotide residues. in the stringently infected hh514cl16 cell line the same cpg residues were methylated in three quarters of the genomes. 5'deoxy-5'-s-isobutyladenosine (siba), a dna methylase inhibitor, was utilized to disrupt the ebv latent st ... | 1989 | 2538121 |
| biological activities and cellular uptake studies of fluorescent derivatives of indole alkaloid tumor promoter teleocidin. | to investigate the interaction between tumor promoters and their cellular targets, 6 new fluorescent derivatives of indole alkaloid tumor promoter teleocidin were synthesized from (-)-indolactam v, and examined for induction of epstein-barr virus, binding ability to the tpa receptor on mouse skin and activation of protein kinase c. (-)-7-(2-n-dansylaminoethyl)indolactam v (dansyl-ilv) had strong activities and proved to be a potent tumor promoter in a 2-stage carcinogenesis experiment. (-)-2-for ... | 1989 | 2538401 |
| characterization of lymphokines mediating b cell growth and differentiation from monoclonal anti-cd3 antibody-stimulated t cells. | addition of anti-cd3 mab 147 (igg1), 446 (igg1), or 454 (igg2a) to cultures of t plus non-t cells can result in both b cell growth and differentiation. to determine whether lymphokines mediating these activities were similar to those described from conventional mitogen-induced t cell activation, normal peripheral blood t cells were stimulated with anti-cd3 mab for 48 h. the supernatants were assayed for factors inducing b cell growth or differentiation (bcdf). a marked increase in ig secretion w ... | 1989 | 2538506 |
| early human igh gene assembly in epstein-barr virus-transformed fetal b cell lines. preferential utilization of the most jh-proximal d segment (dq52) and two unusual vh-related rearrangements. | we have analyzed the phenotypic characteristics and igh gene rearrangements in a panel of ebv-transformed b lineage cell lines from human fetal liver and bone marrow. some lines contained only populations of immature, ig- be cells, while others contained mixed populations of mature and immature b cells. the majority of identifiable igh rearrangements involved joining of the most jh-proximal d segment, dq52, to various jh segments, implying that dq52 is a preferred target for initial djh rearrang ... | 1989 | 2538551 |
| rhabdomyolysis associated with simultaneous epstein-barr virus infection and isolation of echovirus 6 from muscle: a dual infection. | 1989 | 2538573 | |
| a method to identify distinctive charge configurations in protein sequences, with application to human herpesvirus polypeptides. | charge interactions are of great importance for protein function and structure, and for a variety of cellular and biochemical processes. we present a systematic approach to the detection of distinctive clusters, runs and periodic patterns of charged residues in a protein sequence. criteria and formulae are set forth to assess statistical significance of these charge configurations. for the 80-odd proteins potentially encoded by the epstein-barr virus, only the major nuclear antigens of the laten ... | 1989 | 2538622 |
| influence of burkitt's lymphoma and primary b cells on latent gene expression by the nonimmortalizing p3j-hr-1 strain of epstein-barr virus. | the epstein-barr virus (ebv) genes expressed in b lymphocytes immortalized in vitro or in burkitt's lymphoma (bl) cells infected in vivo have been characterized previously; however, the viral products which are essential for immortalization or for establishment of ebv latency are still not known. to approach this question, we compared the kinetics of expression of ebv nuclear antigens and the two ebv-encoded small rnas, eber1 and eber2, after infection of primary b cells or ebv genome-negative b ... | 1989 | 2538644 |
| expression of the bzlf1 latency-disrupting gene differs in standard and defective epstein-barr viruses. | previous experiments using gene transfer of plasmids with heterologous promoters identified an epstein-barr virus (ebv) gene (bzlf1) whose product (zebra) switches the virus from a latent to a replicative state. we have now studied expression of zebra in lymphoid cells harboring either standard virus or a mixture of standard and defective (heterogeneous [het]) viruses. a high-titer rabbit antiserum to a trpe-bzlf1 fusion protein was used to identify zebra expressed from standard and het ebv dna. ... | 1989 | 2538652 |
| the epstein-barr virus (ebv) bzlf1 immediate-early gene product differentially affects latent versus productive ebv promoters. | the epstein-barr virus (ebv) bzlf1 gene product is thought to mediate the disruption of latent ebv infection. we have examined the regulatory effects of bzlf1 by studying its transactivating effects on seven different ebv promoters. we find that whereas the bzlf1 gene product increases the activity of the two early promoters, bmlf1 and bmrf1, it decreases the activity of three latent promoters (the bamhi-c and bamhi-w epstein-barr nuclear antigen promoters and the latent membrane protein promote ... | 1989 | 2538653 |
| immunoglobulin and t-cell receptor gene rearrangements in hodgkin's disease and ki-1-positive anaplastic large cell lymphoma: dissociation between phenotype and genotype. | we have determined the tumor cell immunophenotype and the rearrangement configuration of immunoglobulin and t-cell receptor genes in 39 cases of hodgkin's disease (hd), six hd-derived cell lines and 22 cases of ki-1-positive anaplastic large cell lymphomas (ki-1-alc). rearrangements were observed in 11/39 hd cases, 15/22 ki-1-alc, and all cell lines. epstein-barr virus dna was found in five hd cases, one cell line, and one ki-1-alc. both hd and ki-1-alc frequently displayed a dissociated genotyp ... | 1989 | 2538681 |
| improved ebv shuttle vectors. | shuttle vectors based on epstein-barr virus (ebv) replicate autonomously in the nuclei of human cells. these vectors represent reasonable models for chromosomes, have low background mutation frequencies, and have been useful for studying induced mutation in human cells. two improvements in the ebv vector system are discussed. attempts are described to increase vector copy number per cell by using a limited period of replication driven by the simian virus 40 (sv40) origin of replication. isolatio ... | 1989 | 2538736 |
| comparative study of epstein-barr virus and sv40-based shuttle-expression vectors in human repair-deficient cells. | shuttle vectors and expression vectors have been used in human cells to examine various aspects of dna repair including effects of dna damage on mutagenesis, transcription, replication and recombination. a combined shuttle-expression system should provide further advantages for the stable expression of and perhaps selection/rescue strategies for dna repair genes. we describe 2 such systems. the first is a simian virus 40 (sv40) shuttle system which allows a quasi-stable episomal vector/host rela ... | 1989 | 2538738 |
| epstein-barr virus. chance and felicity. | 1989 | 2538759 | |
| epstein-barr virus episome-based promoter function in human myeloid cells. | epstein-barr virus (ebv) episomal replicons offer an expeditious means for amplifying transfected genes in human cells. a panel of ebv episomes was constructed to assess the relative utility of five distinct eukaryotic promoter elements for high level and inducible gene expression in stably transfected human myeloid leukemia cells. the rous sarcoma virus 3' long terminal repeat (ltr) was most highly suited for ebv episome-based gene expression, whereas the lymphopapilloma virus and the sv40 earl ... | 1989 | 2538801 |
| overexpression of the human bcl-2 gene product results in growth enhancement of epstein-barr virus-immortalized b cells. | the biological activity of the human bcl-2 gene product was analyzed in an epstein-barr virus (ebv)-infected human lymphoblastoid b-cell line transfected with bcl-2 sequences driven by the simian virus 40 promoter and enhancer. overproduction of the bcl-2 protein conferred a selective growth advantage to the ebv-infected b cells as selective growth advantage to the ebv-infected b cells as compared with control transfectants in low-serum medium and also after seeding at limiting dilution but did ... | 1989 | 2538824 |
| light-chain gene expression before heavy-chain gene rearrangement in pre-b cells transformed by epstein-barr virus. | epstein-barr virus transformation of b-cell-depleted bone marrow cells from human fetuses allowed us to identify novel cell types characterized by the expression of immunoglobulin kappa or lambda light chain without heavy chains. four kappa-only clones with normal karyotype were obtained and examined for their immunoglobulin gene configurations and expression. all four clones had kappa-chain gene rearrangements at either one or both alleles, but the heavy-chain gene loci in these clones either w ... | 1989 | 2538839 |
| complement receptors. | 1989 | 2538973 | |
| epstein-barr virus in the sublabial salivary gland in sjögren's syndrome. | the proposed role of epstein-barr virus (ebv) in salivary gland destruction in sjögren's syndrome (ss) prompted the authors to study the presence of ebv-dna (hybridohistochemistry) and ebv-encoded proteins (immunohistochemistry) in sublabial salivary glands taken from eight patients with primary and five with secondary ss and from 16 controls. dna probes and anti-ebv antibodies were controlled for activity by assessment of human blood b-lymphocytes after in vitro infection with ebv. none of the ... | 1989 | 2539008 |
| enhancement of epstein-barr virus/c3d receptor (ebv/c3dr or cr2) and nuclear p120 ribonucleoprotein phosphorylation by specific ebv/c3dr ligands in subcellular fractions of the human b lymphoma cell line, raji. | we present herein the first evidence that interaction of specific ebv/c3dr ligands, as human c3bi/c3d and anti-ebv/c3dr moab, with ebv/c3dr enhanced significantly, in a dose dependent process, phosphorylation of ebv/c3dr and p120 rnp present in subcellular fractions, as purified plasma membranes and nuclei, of the human b lymphoma cell line, raji. the use of kinase effectors allowed to detect some of the kinases involved in these phosphorylations. pp60src-like phosphotyrosine kinase and protein ... | 1989 | 2539145 |
| inhibition of polyamine synthesis in human b lymphocytes during primary infection with epstein-barr virus (ebv) blocks cellular dna synthesis but not the expression of ebv-encoded nuclear antigens (ebna). | inhibition of polyamine synthesis by 2-difluoromethylornithine (dfmo) treatment had no apparent effect on the initial manifestation of epstein-barr virus (ebv) infection in human b lymphocytes, because the expression of ebv-encoded nuclear antigens (ebna) occurred normally. however, many subsequent steps in the transformation process were inhibited by dfmo treatment. these include cellular dna synthesis and immunoglobulin (igm, igg and iga) synthesis and secretion. consequently, dfmo treatment b ... | 1989 | 2539156 |
| human monoclonal anti-d antibodies. ii. the relationship between igg subclass, gm allotype and fc mediated function. | eight monoclonal antibodies (mabs) to the rh antigen d produced by epstein-barr virus transformed b-lymphoblastoid cell lines from two individuals have been compared for their behaviour in in vitro cell-mediated assays. three igg1 glm(1,17) and two igg3 g3m(21) mabs from one donor and three igg1 glm(3) mabs from another were used. igg3 anti-d mabs induced greater adherence and phagocytosis of sensitized red cells by u937 monocytes than igg1 anti-d mabs or the polyclonal anti-d. minimum sensitiza ... | 1989 | 2539182 |
| properties of glucocorticoid receptors in epstein-barr virus-transformed lymphocytes from patients with familial cortisol resistance. | in a previous report of two patients with familial glucocorticoid resistance due to reduced numbers of glucocorticoid receptors (gr), we have shown decreased numbers of gr in peripheral mononuclear cells and cultured fibroblasts but normal affinity of gr in both patients. in this study, peripheral lymphocytes from these patients, one patient's son and daughter, and normal subjects were transformed with epstein-barr virus. reduced numbers and normal affinity of gr were found in the epstein-barr v ... | 1989 | 2539255 |
| an intricate route to immortality. | 1989 | 2539260 | |
| epstein-barr-virus-transformed lymphoblastoid cell lines derived from patients with x-linked agammaglobulinaemia and wiskott-aldrich syndrome: responses to b cell growth and differentiation factors. | epstein-barr-virus-transformed b lymphoblastoid cell lines (ebv-transformed lcl) from three patients with x-linked agammaglobulinaemia (xla), six patients with wiskott-aldrich syndrome (was), and seven normal donors, were tested for growth and differentiation in response to human recombinant il-4, a commercially available, low molecular weight b cell growth factor (bcgflow), and b cell differentiation factor (bcdf) secreted by the t24 cell line, now known to be il-6. proliferation (3h-tdr uptake ... | 1989 | 2539277 |
| changes in the phenotype and immunoglobulin secretion of human b cells following co-culture with cells of an ebv+ lymphoblastoid line or fusion with mouse plasmacytoma cells. studies in short-term and long-term culture. | the mechanisms controlling immunoglobulin production have been studied in two types of immunoglobulin-secreting cell generated from human b cells. the first type (i) was produced by activation and transformation of b cells by co-culture with cells of an epstein-barr-virus-positive (ebv+) lymphoblastoid cell line (ebv-b-lcl). the second type (ii) consisted of human/mouse hybrid cells produced by fusing human tonsil b cells with cells of a mouse plasmacytoma line. both these methods, singly and in ... | 1989 | 2539279 |
| persistence of epstein-barr virus in salivary gland biopsies from healthy individuals and patients with sjögren's syndrome. | salivary gland biopsies from 12 patients with primary sjögren's syndrome and 10 controls were examined for epstein-barr virus (ebv) dna by in-situ hybridization and for ebv proteins by immunofluorescence and peroxidase techniques. viral dna was found in biopsies from two out of 12 patients with primary sjögren's syndrome and six out of the 10 controls. the dna and early antigen were in epithelial cells lining the ducts and acini, early antigen expression being limited to the luminal side of the ... | 1989 | 2539280 |
| posttransplant lymphoproliferative disorders in heart-lung transplant recipients: primary presentation in the allograft. | heart-lung transplant recipients are predisposed to acute rejection episodes, bronchiolitis obliterans, and opportunistic infections. in 9.4% of recipients at the university of pittsburgh, a posttransplant lymphoproliferative disorder (ptld) developed, and in 60% of cases, it presented in the allografted lungs and was associated with primary infection by epstein-barr virus (ebv). the ptld is histologically indistinguishable from a primary pulmonary lymphoma and consists of a mixed population of ... | 1989 | 2539321 |
| high interferon titer and defective nk-cell activity in the circulation of nasopharyngeal carcinoma patients. | the interferon (ifn) activity of sera from 19 patients with nasopharyngeal carcinoma (npc) was determined by the plaque-reduction assay with vesicular stomatitis virus (vsv) in hela cells and compared to that of sera from matched healthy controls. high titers of interferon were detected in the sera of the npc patients with a geometric mean titer (gmt) of 43 +/- 25 u/ml. the interferon activity of the patients' sera was acid- and heat-labile (ph = 2 and 56 degrees c for 1 hr) and could be neutral ... | 1989 | 2539326 |
| limiting dilution cloning of b cells from patients with multiple myeloma: emergence of non-malignant b-cell lines. | multiple myeloma (mm) is a b-cell malignancy characterized by the accumulation of slowly proliferating malignant plasma cells in the bone marrow (bm). several reports have shown the existence of an abnormal b-cell compartment including proliferative idiotypic b cells (i.e., b cells bearing the same idiotypic determinants as the myeloma protein) in the bm and peripheral blood (pb) of patients with mm. in order to study whether this abnormal compartment can be grown in vitro, we cultured the pb an ... | 1989 | 2539329 |
| sister chromatid exchange and nasopharyngeal carcinoma. | sister chromatid exchange (sce) is a genetic indicator of dna damage in mammalian cells and may afford a sensitive monitor to follow genomic instability of some individuals with fragile chromosomal diseases or malignancies. in studies on the effect of dinitrosopiperazine (dnp), aflatoxin b1 (afb1), methyl-nitro-nitroso-guanidine (mnng) and epstein-barr virus (ebv) infection on sce in lymphocytes from nasopharyngeal carcinoma (npc) patients, we found that: (1) the spontaneous sces in peripheral b ... | 1989 | 2539330 |
| epstein-barr virus and a tumour-promoting phorbol ester use similar mechanisms in the stimulation of human b-cell proliferation. | in marked contrast to ligands which activate b cells via their physiological receptors for antigen, transforming epstein-barr virus (ebv) was found to be mitogenic for human b lymphocytes without increasing inositol phospholipid hydrolysis. b-cell stimulation by ebv showed similar characteristics to those achieved by the tumour-promoting phorbol ester tpa, in terms of the temporal appearance of surface activation antigens, the induction of rna and dna synthesis and the lower requirement for medi ... | 1989 | 2539333 |
| interferon-gamma in a family with x-linked lymphoproliferative syndrome with acute epstein-barr virus infection. | a 20-month-old male with fulminant infectious mononucleosis and the x-linked lymphoproliferative syndrome (xlp) was studied. epstein-barr virus (ebv)-determined nuclear antigen (ebna) and ebv dna were detected in various tissues. despite a combined treatment with acyclovir, immunoglobulin, and methylprednisolone, the patient deteriorated rapidly. following treatment with recombinant interferon-gamma (ifn-gamma), defervescence occurred and circulating ebna-positive cells markedly decreased. ifn-g ... | 1989 | 2539385 |
| epstein-barr virus gene expression in malignant lymphomas induced by experimental virus infection of cottontop tamarins. | inoculation of cottontop tamarins with a large dose of epstein-barr virus (ebv) leads to the induction of multiple ebv genome-positive lymphomas. these tumors have been characterized as oligoclonal or monoclonal large-cell malignant lymphomas that closely resemble the ebv genome-positive b-cell lymphomas that arise in human allograft recipients. the expression of latent and lytic ebv-encoded proteins was investigated in these virus-induced tamarin lymphomas and in derived cell lines. the tamarin ... | 1989 | 2539497 |
| physical mapping and nucleotide sequence of a herpes simplex virus type 1 gene required for capsid assembly. | in this report, we describe some phenotypic properties of a temperature-sensitive mutant of herpes simplex type 1 (hsv-1) and present data concerning the physical location and nucleotide sequence of the genomic region harboring the mutation. the effect of shifts from the permissive to the nonpermissive temperature on infectious virus production by the mutant a44ts2 indicated that the mutated function is necessary throughout, or late in, the growth cycle. at the nonpermissive temperature, no majo ... | 1989 | 2539510 |
| antiidiotypic antibodies: production in vitro by ebv-transformed lymphoblastoid cell lines and immunochemical characterization of idiotypic receptors on t-cells. | 1989 | 2539669 | |
| specific immunological tolerance/optimal graft function in the absence of immunosuppressive therapy. | 1989 | 2539670 | |
| comparison of dithiothreitol (dtt) treatment and absorption using autologous ebv-transformed lymphoblastoid cell lines for the depletion of autoreactive antibodies. | 1989 | 2539673 | |
| a study of epstein-barr virus infections in heart and heart and lung transplant recipients. | 1989 | 2539676 | |
| persistence and transfer of epstein-barr virus after allogeneic bone marrow transplantation. | 1989 | 2539687 | |
| cotransfection of nucleic acid segments by sendai virus envelopes. | it is reported here that sendai virus envelopes (sve) can be used to transfect multiple copies of dna segments of different varieties and size. this capability further increases the usefulness of sve. in addition, the ability to simultaneously transfect multiple copies of different genome segments promises to be a powerful tool in the field of molecular biology. the simultaneous transfection of neo gene and cytomegalovirus immediate early antigen gene was successfully done. sendai virus envelope ... | 1989 | 2539842 |
| infection and rheumatic diseases. | 1989 | 2539930 | |
| human anti-hla-dqw2 monoclonal antibody secreted by an epstein-barr-virus--transformed lymphoblastoid cell line: assessment of the monoclonality, allospecificity, and target. | igm molecules were purified by the use of anti-igm antibody-coupled sepharose from the culture supernatant of an epstein-barr-virus-transformed lymphoblastoid cell line, mp1, that secretes alloantibodies possessing hla-dqw2 specificity as defined by the cytotoxicity assay. the obtained igm preparation was labeled with radioactive iodine-125i and fractionated by gel filtration. it contained pentameric igm and smaller oligomeric igms. when tested by the direct cellular binding assay against a pane ... | 1989 | 2540128 |
| active epstein-barr virus infection in post-viral fatigue syndrome. | serological evidence of active infection with epstein-barr virus (ebv) was found in 25 of 124 patients (20%) with the post-viral fatigue syndrome (pvfs). in another study on the same group of patients around 50% were found to have evidence of chronic enterovirus infection. no overlap was found between those patients with enterovirus infection and those with active ebv infection. we suggest that there are multiple causes of pvfs and that, in the absence of coexisting immunosuppressive disease whi ... | 1989 | 2540242 |
| [cytomegalovirus infection acquired in the netherlands and in the tropics; a retrospective study in previously healthy adults]. | clinical and laboratory data of 44, previously healthy patients with cytomegalovirus (cmv) infections were analysed in a retrospective study (1975-1986). eighteen (41%) were probably infected in the (sub)tropics. 33 (75%) patients showed symptoms and signs of cmv-mononucleosis: fever, malaise, absolute and relative lymphocytosis with atypical lymphocytes and liver function disturbance without jaundice. enlargement of lymph-nodes was present in only 20% of the patients, distinct from mononucleosi ... | 1989 | 2540440 |
| epstein-barr virus (ebv) infection by cocultivation of fibroblast-dominant cell line and ebv-transformed lymphocytes. | a long-term cell culture epitheloid cell line (npc-f/l) was established from the fibroblast-dominant cell line derived from nasopharyngeal carcinoma tissue by cocultivation with an epstein-barr virus (ebv)-transformed lymphoblastoid cell line of adenoid origin. the cells were investigated by karyotyping, by immunofluorescent testing for ebv-associated nuclear antigen (ebna), early antigen (ea), and viral capsid antigen (vca) and by a light microscopy. the cells were shown to be epithelial not on ... | 1989 | 2540473 |
| spontaneous lymphoblastoid transformation of cells obtained from human tonsils. | spontaneous lymphoblastoid transformation of cells obtained from 25 human tonsils were investigated. three epstein-barr virus (ebv) genome-positive lymphoblastoid cell lines were spontaneously established in tissue cultures in vitro from human tonsils. in addition, the frequency of spontaneous lymphoblastoid transformation was increased by the treatment of tumor promoter, 12-0-tetradecanoylphorbol-13-acetate and 18 lymphoblastoid cell lines were established. the data suggest that the human tonsi ... | 1989 | 2540474 |
| epstein-barr virus-associated lymphoproliferative disorder. applications of immunoperoxidase and molecular biologic techniques. | a 17-year-old boy with fever and cervical lymphadenopathy developed multiple-organ failure and died three weeks after hospital admission. a lymph node biopsy specimen demonstrated a florid immunoblastic infiltrate that was suspicious for a malignant lymphoma. by using immunoperoxidase and molecular biologic techniques, evidence was presented for an epstein-barr virus-associated lymphoproliferative disorder. | 1989 | 2540726 |
| opsoclonus secondary to an epstein-barr virus infection. | 1989 | 2540736 | |
| [ebv-specific antibodies in patients with nasopharyngeal cancer and tonsillar cancer. follow-up of over 4 years]. | 26 npc patients and 47 tc patients were investigated clinically, and in the sera of these patients ebv-specific antibodies were determined. no relapse occurred in 11 patients with npc and in 17 patients with tc during the period of 4 years. in addition to the determination of antibodies we looked for ebna in 3 undifferentiated npc and 4 undifferentiated tc, and for ebv-dna in 2 of these npc and one of these tc. the 3 npc and 4 tc tested for ebna showed this antigen in the cells. 2 npc and 1 tc s ... | 1989 | 2540762 |
| analysis of human ks biopsies and cloned cell lines for cytomegalovirus, hiv-1, and other selected dna virus sequences. | investigation into a possible role of several human viruses, including human cytomegalovirus (hcmv), human immunodeficiency virus (hiv-1), hepatitis b virus (hbv), human herpesvirus 6 (hhv6), and epstein-barr virus (ebv) in the pathogenesis of kaposi's sarcoma (ks) has resulted in the lack of an association of these viruses in ks biopsy and cloned ks cell line specimens. since nearly all patients with ks, including those with hiv-associated ks, have a high incidence of hcmv infection, hcmv has b ... | 1989 | 2540783 |
| extranodal noncutaneous lymphoid hyperplasias represent a continuous spectrum of b-cell neoplasia: demonstration by molecular genetic analysis. | we investigated 16 lymphoid proliferations occurring in the ocular adnexa, salivary glands, breast, and thyroid gland and satisfying the histopathologic and immunophenotypic criteria of benign lymphoid hyperplasia for the presence of clonal rearrangements of the antigen receptor, c-myc, bcl-1, and bcl-2 genes and epstein-barr virus (ebv) dna sequences. each of these 16 extranodal, noncutaneous lymphoid neoplasms exhibited clonal immunoglobulin heavy and/or light chain and lacked t-cell receptor ... | 1989 | 2540855 |
| epstein-barr virus bzlf1 trans-activator specifically binds to a consensus ap-1 site and is related to c-fos. | two regions of the epstein-barr virus bzlf1 trans-activator protein have sequence similarity to the c-fos protein. part of the similarity corresponds to the region of c-fos which is similar to the dna binding domain of c-jun and gcn-4. the structure of the exon which contains this region in c-fos and bzlf1 is also highly conserved between the two genes. complete bzlf1 protein and a c terminal fragment were prepared either as purified fusion proteins or by in vitro translation from a bzlf1 cdna. ... | 1989 | 2540954 |
| the generation of hybridomas secreting human monoclonal antibodies reactive with type ii collagen. | human monoclonal antibodies reactive with type ii collagen were obtained from patients with rheumatoid arthritis and relapsing polychondritis by fusion of cells with the human-mouse myeloma analogue hmma2.11 tg/0. direct fusion of peripheral blood or bone marrow mononuclear cells was unsuccessful in obtaining antibody producing hybridomas although fusion efficiency was high (1 hybridoma per 25,400 mononuclear cells fused). polyclonal, epstein barr virus transformed b cell lines derived from peri ... | 1989 | 2541068 |
| the effect of leucyl-leucine methyl ester on proliferation and ig secretion of ebv-transformed human b lymphocytes. | the selective cytotoxicity of the lysosomotropic methyl esters of leucine or its lysosomal condensation product leucyl-leucine has been used to investigate the effect of cytolytic cells on the clonal outgrowth, cellular proliferation and antibody secretion of epstein-barr virus (ebv)-transformed human b cells. large granular lymphocytes (lgl), monocytes, and a subset of t cells (cd8/cd11+) were permanently eliminated by the ester treatment. these lysosome-rich cells severely inhibit the clonal o ... | 1989 | 2541070 |
| ebv, molecular mimicry and rheumatoid arthritis: a hypothesis. | 1989 | 2541079 | |
| interleukin-2 induced killer cell activity against epstein-barr virus-immortalized human b cells. | interleukin-2 (il-2) activated killer (lak) cells, generated in vitro by treating peripheral blood lymphocytes (pbl) with human il-2, are able to lyse a wide variety of target cells without restriction by major histocompatibility complex (mhc) molecules. earlier observations from this and other laboratories indicated that patients with epstein-barr virus (ebv) induced infectious mononucleosis, a self-limiting viral disease, have high ebv-non-specific natural killer (nk) cell activity. since the ... | 1989 | 2541081 |
| dna sequence analysis of the hla-drw12 allele. | the complete dna sequence of a dr beta chain cdna encoding the drw12 allele has been determined. the sequence of this drb1 allele reveals a structural relationship to the group of other drb1 genes found on drw52 haplotypes, such as dr3, -w11, -w13, -w14, and -w8. the structural similarities among this group of alleles are particularly evident in the first hypervariable as well as in the 3' untranslated region. the second hypervariable region contains a unique sequence not identified in any other ... | 1989 | 2541120 |
| impaired late suppression of epstein-barr virus (ebv)-induced immunoglobulin synthesis: a common feature of autoimmune disease. | we examined regulation of epstein-barr virus-induced plaque-forming cell generation in peripheral blood mononuclear cells from several autoimmune and seronegative diseases and correlated these results with epstein-barr virus-induced proliferation. we confirmed the defective regulation of epstein-barr virus-induced plaque-forming cells in peripheral blood mononuclear cells of patients with rheumatoid arthritis and scleroderma. peripheral blood mononuclear cells from patients with seronegative art ... | 1989 | 2541162 |
| cytotoxic t lymphocytes specific for self tumor immunoglobulin express t cell receptor delta chain. | ctl are thought to play a role in the elimination of transformed cells in vivo. the effectiveness of such ctl is in part dependent on recognition of tumor specific antigens. among the best characterized tumor-specific antigens are the unique or idiotypic determinants on the ig of b cell lymphomas. here we describe the generation and properties of human ctl specific for the idiotype on autologous b cell tumors. these cells are cd3+,cd4-,cd8- and express the delta chain of the tcr. such cells may ... | 1989 | 2541219 |
| relationship of variable region genes expressed by a human b cell lymphoma secreting pathologic anti-pr2 erythrocyte autoantibodies. | to study the biology of cold agglutinin disease we previously established ebv-transformed b cell clones isolated from a patient with splenic lymphoma of an early plasmacytic cell type and immune hemolysis due to an anti-pr2 cold agglutinin. these clones had an aberrant chromosomal marker identical to the patient's b cell lymphoma and each secreted igmk anti-pr2 similar to the pathologic autoantibody in the serum of the patient. in this study, we have further investigated the pr2-specific autoimm ... | 1989 | 2541221 |
| ebv. managing the physiological and psychosocial implications of the epstein-barr virus. | ebv has both physiological and psychological implications. few medical/nursing interventions are available to alleviate the physical stressors and fatigue. the primary nursing intervention, therefore, becomes one of providing emotional support and psychosocial interventions. providing measures to ensure adequate sleep will assist the patient in more positive feelings about self. although causation and diagnosis of this syndrome may be debated in the medical literature, the nursing role is to pro ... | 1989 | 2541243 |
| identification of interleukin 1 alpha produced by the 3b6 human ebv-b cell line. | we have previously described a novel protein, derived from the 3b6 ebv b-cell line which apparently displayed il-1 bioactivity. we report here that the protein previously characterized is not a new species of il-1. indeed, we have now been able to clone and express a cdna which identifies this protein as human thioredoxin, an intracellular dithiol oxydo-reductase enzyme. thioredoxin however, is not a lymphokine and in particular does not possess il-1 activity. our earlier data can be explained i ... | 1989 | 2541284 |
| epstein-barr virus. synthesis for a complex role. | 1989 | 2541346 | |
| [detection of epstein-barr viruses in rasmussen's encephalitis. report of 2 cases]. | 1989 | 2541350 | |
| characterization of n- and o-linked oligosaccharides of glycoprotein 350 from epstein-barr virus. | glycoprotein 350 (gp350), the major epstein-barr virus (ebv) envelope glycoprotein, has extensive n- and o-linked oligosaccharide chains. to characterize these oligosaccharide chains, [3h]glucosamine-labeled gp350 was isolated from an ebv transformed marmoset lymphoblastoid cell line (b95-8) induced to replicate ebv. radiolabeled pronase-glycopeptides were fractionated by serial affinity chromatography and o-linked oligosaccharides released by mild alkaline borohydride treatment. virtually all ( ... | 1989 | 2541535 |
| cycloheximide-resistant gene of epstein-barr virus in freshly infected b lymphocytes. | the transcription of latent epstein-barr viral (ebv) genes, i.e., genes encoding ebna-1, ebna-2, ebna-3a, -3b, -3c, and lmp, were detected in human tonsillar lymphocytes early after infection with ebv. transcription of the bhlf-1 open reading frame was also detected at this initial phase of immortalization. cycloheximide treatment inhibited the transcription of all the latent ebv genes but not bhlf-1. these results suggest that bhlf-1 might be considered an immediate-early gene of ebv. cyclohexi ... | 1989 | 2541549 |
| neutralization of epstein-barr virus-induced ribonucleotide reductase with antibody to the major restricted early antigen polypeptide. | a previous study had established a relationship between the major polypeptide of the epstein-barr virus (ebv)-induced restricted (r) component of the early antigen (ea) complex and the large subunit of ribonucleotide reductase. this association was confirmed in this study by the observation that a monoclonal antibody prepared against the 85-kda r component reacted in immunoblotting with the protein product (molecular weight approximately 93 kda) encoded by the xbal fragment of the b-95-8 strain ... | 1989 | 2541553 |
| epstein-barr virus dna in nasopharyngeal biopsies. | the epstein-barr virus (ebv) has been closely associated with the undifferentiated form of nasopharyngeal carcinoma (npc), which is particularly common in the high risk area in southeast china. we have examined 37 nasopharyngeal biopsies from patients within this high risk area, including 31 cases of undifferentiated npc and 6 cases of patients with nasopharyngitis, for the presence of ebv dna. we found that 26 of 31 biopsies from npc patients were ebv dna positive; 3 of the 6 biopsies from pati ... | 1989 | 2541582 |
| undifferentiated carcinoma of nasopharyngeal type of tonsil. | a review of 2262 squamous cell carcinomas of the tonsillar region seen at the institut gustave-roussy, villejuif, france, from 1970 to 1986 showed 1837 well- and poorly differentiated squamous cell carcinomas and 425 undifferentiated squamous cell carcinomas. eighteen patients with undifferentiated squamous cell carcinomas presented histologic characteristics of undifferentiated carcinomas of nasopharyngeal type. radiosensibility and radiocurability (complete sterilization with 70 gy administere ... | 1989 | 2541744 |
| human monoclonal antibody against a gag-coded protein of human immunodeficiency virus produced by a stable ebv-transformed cell clone. | an ebv-transformed lymphoblastoid b cell clone (a12) derived from peripheral blood lymphocytes of an hiv-1-infected individual is described. the immunoglobulin isotype produced by this clone was igm, and southern blot analysis of immunoglobulin gene rearrangement showed a monoclonal pattern. the a12 monoclonal antibody was specific for the p24 product of the hiv-1 gag gene. this clone is now in continuous culture for more than 8 months and no changes in its biologic properties have been observed ... | 1989 | 2541750 |
| highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization. | use of in situ hybridization optimized for fluorescent detection of nuclear rna has revealed a striking localization of specific viral rnas within nuclei of cells latently infected with ebv. several hundred kb of specific transcripts is sharply restricted to a small region of the nucleus, frequently in a curvilinear "track". detection of nuclear rna was evidenced by hybridization without denaturation, sensitivity to rnaase, inhibition by actinomycin d, and specificity of transcribed sequences. r ... | 1989 | 2541917 |
| epstein-barr virus small rna (eber) genes: unique transcription units that combine rna polymerase ii and iii promoter elements. | the epstein-barr virus-encoded small rna (eber) genes appear to comprise an interesting subset of class iii genes different from any previously identified, including u6 and 7sk. eber genes have functional a and b box intragenic control regions. in addition, they contain three upstream elements that together stimulate in vivo expression 50-fold and resemble sites associated with typical class ii promoters. dnaase i footprinting analyses using purified proteins or oligonucleotide competition demon ... | 1989 | 2541926 |
| allele-specific down-regulation of mhc class i antigens in burkitt lymphoma lines. | we have reported that burkitt lymphomas (bl) that arise in hla-a11 positive individuals are resistant to lysis by hla-a11-specific and hla-a11-restricted ctls(10,11). here we show that this phenomenon can be explained by a selective loss of the hla-a11 polypeptide. the hla-a11 negative phenotype is due to a regulatory phenomenon, rather than a structural defect, as proven by the ability to rescue expression of hla-a11 in in vitro epstein-barr virus (ebv)-converted sublines of ebv negative bls. | 1989 | 2541930 |
| epstein-barr virus-transformed human b lymphocytes endocytose autologous and heterologous red blood cells. | epstein-barr virus (ebv)-transformed b lymphocytes, either isolated from a patient with ebv-induced infectious mononucleosis or obtained by in vitro infection of b lymphocytes of donors in different clinical conditions, have been tested for the ability to endocytose particulate forms of antigens, such as human, sheep or mouse red blood cells. by light and electron microscopy, it has been found that ebv-transformed b cells are able to bind and internalize human autologous and allogeneic erythrocy ... | 1989 | 2542165 |
| phenotypic and cellular changes associated with loss of antigen-induced function in epstein-barr virus-specific human t-cell hybrids. | human t-cell hybrids were constructed from the hat-sensitive human t-cell line 'jmae' and an uncloned population of t cells from donor sw (sw-t; partner cell) known to have strong specificity for the autologous epstein-barr virus (ebv)-transformed b cell, swebv. the resulting hybrids, 1a9, 1d12 and 2c8 were shown to have similar specificity and to increase their rate of proliferation in response to swebv. furthermore, the hybrids responded to other suitable ebv-transformed targets, in an major h ... | 1989 | 2542166 |
| occurrence of epstein-barr virus deoxyribonuclease enzyme activity-neutralizing antibodies. an index of epstein-barr virus disease. | the occurrence of epstein-barr virus (ebv) specific dnase antibodies was measured in the sera of 400 patients. three hundred sera were positive (greater than or equal to 20) for early antigen diffuse component (ea-d) antibodies, as measured by indirect immunofluorescence (if). one hundred sera were ea-d antibody-negative but had if igg titers (greater than 40) to the viral capsid antigen (vca). twenty-nine of the anti-ea-d-positive sera, and 14 of the anti-ea-d-negative sera showed specific inhi ... | 1989 | 2542178 |
| gene mapping of an epstein-barr virus isolate obtained from a nasopharyngeal carcinoma. | gene mapping of an epstein-barr virus (ebv) isolate derived from a nasopharyngeal carcinoma (npc), designated npc-ebv, has been performed. this isolate was rescued from an npc epithelial hybrid cell line (npc-kt), and used to transform cotton-top tamarin lymphocytes which, along with the epithelial npc-kt cells, were used in the mapping studies. using the bamhi and ecori restriction enzymes, we found that the npc-ebv isolate did not contain the deletions observed in the genomes of the prototype ... | 1989 | 2542179 |
| intracellular synthesis of epstein-barr virus membrane antigen gp350/220. inhibitory effect of monensin on its expression. | we have defined the intracellular expression and localization of gp350/220, one of the epstein-barr virus (ebv) induced membrane antigens, on 12-o-tetradecanoyl-phorbol-13-acetate (tpa) and n-butyrate-treated p3hr-1 cells. 1b6 monoclonal antibody (mab) immunoprecipitated gp350/220 from [35s]-methionine-labeled cells, as confirmed with other mabs (2l10, 72a1, and c1), to the same membrane antigen. the appearance of gp350/220 was observed about 14 h after tpa and n-butyrate activation and reached ... | 1989 | 2542183 |
| clonal t-cell lymphoproliferation containing epstein-barr (eb) virus dna in a patient with chronic active eb virus infection. | a 10-year-old boy with chronic active epstein-barr virus (ebv) infection developed t-cell lymphoproliferation in the terminal stage of hepatic failure. the phenotypes of the proliferating lymphocytes were cd3+, cd4+ and hla-dr+. the genomic dnas from these cells demonstrated two rearranged t-cell receptor beta-chain genes and contained the ebv genome. these findings indicate that ebv can infect t lymphocytes and might cause clonal t-cell lymphoproliferation. | 1989 | 2542204 |
| molecular characterization of a major autoantibody-associated cross-reactive idiotype in sjogren's syndrome. | primary sjogren's syndrome is an autoimmune disorder characterized by lymphocytic infiltration of the salivary and lacrimal glands, producing associated dry eyes (keratoconjunctivitis sicca), dry mouth, and intermittently swollen salivary glands. a high proportion of the infiltrating b lymphocytes express surface and cytoplasmic ig bearing a kappa-l chain-associated cri defined by reactivity with the murine mab, 17.109. to determine the structural basis for cri expression in this disease, we gen ... | 1989 | 2542406 |
| antibody to epstein-barr virus-specific dnase as a marker for field survey of patients with nasopharyngeal carcinoma in taiwan. | a serological survey using antibody to epstein-barr virus (ebv)-specific dnase activity as a marker for the identification of patients with nasopharyngeal carcinoma (npc) has been carried out on healthy subjects who visited government employees' clinic center (gecc) for routine health examination and on individuals residing in npc high-risk areas (hra) in taiwan. during a 3-year prospective study, 22,596 and 9,869 sera were collected from the gecc and hra groups, respectively. taking neutralizat ... | 1989 | 2542436 |
| heterophile igm, iga, and ige antibodies in infectious mononucleosis. | fifty infectious mononucleosis (im) and 150 non-infectious mononucleosis (non-im) sera were tested by a hemadsorption immunocapture test (hit) for the detection of heterophile antibodies of igm, iga, and ige classes. the specificity of paul-bunnell (pb) antibodies was ascertained by a differential absorption test. igm pb-antibodies were demonstrated in 100% of im sera, iga in 92%, and ige in 88%. pb antibodies were present only in im sera; but other heterophile antibodies of igm (68%), iga (6.7% ... | 1989 | 2542444 |
| shuttle vectors for studying mutagenesis in mammalian cells. | shuttle vectors are dna plasmids able to replicate in both mammalian cells and bacteria. they have been used to examine rapidly various aspects of dna repair, recombination and mutagenesis. three main classes of shuttle vector have been developed. the transiently replicating vectors are usually based on simian virus 40 replication origin. the episomal vectors based on the epstein-barr virus replication replicate almost permanently in host cells. different biological systems, including retroviral ... | 1989 | 2542504 |
| mucha-habermann's disease and arthritis: possible association with reactivated epstein-barr virus infection. | we present a 12-year-old girl with skin lesions, arthritis and clinical response to tetracycline consistent with mucha-habermann's disease. she also showed serological evidence of reactivated epstein-barr virus (ebv) infection. we believe this represents the first such association of mucha-habermann's disease with ebv infection. | 1989 | 2542544 |
| chronic ebv infection and pmr. | 1989 | 2542547 | |
| the transforming domain alone of the latent membrane protein of epstein-barr virus is toxic to cells when expressed at high levels. | a previously unrecognized activity has been associated with the product of the bnlf-1 gene of epstein-barr virus. this gene encodes the latent membrane protein of epstein-barr virus. when the gene was expressed at high levels, it was toxic to all cell lines tested, which included six human b-lymphoid lines as well as balb/3t3, 143/ebna-1, and hep-2 cells. the bnlf-1 gene was previously shown to induce anchorage-independent and tumorigenic growth in rat-1 and balb/3t3 cells. we demonstrate here t ... | 1989 | 2542565 |
| a promoter of epstein-barr virus that can function during latent infection can be transactivated by ebna-1, a viral protein required for viral dna replication during latent infection. | a viral promoter that functions on recombinant plasmids in cells immortalized by epstein-barr virus was identified and characterized. it is identical to that mapped on the viral genome by bodescot et al. (m. bodescot, m. perricaudet, and p.j. farrell, j. virol. 61:3424-3430, 1987) which functions during the latent phase of the viral life cycle in some but not all cells to encode several latent viral gene products. experiments with these plasmids indicated that this promoter requires the enhancer ... | 1989 | 2542577 |
| multiple ebna1-binding sites are required to form an ebna1-dependent enhancer and to activate a minimal replicative origin within orip of epstein-barr virus. | ebna1 activates the ebv plasmid maintenance sequence orip by binding to its two essential regions. one region is a family of 30-base-pair (bp) repeats and is activated by ebna1 to act as a transcriptional enhancer. the other region contains a 65-bp dyad symmetry and lacks enhancer function. to explore the functional differences between the two regions, we determined orip activities as functions of the number of 30-bp repeats and compared them with activities determined when tandem copies of the ... | 1989 | 2542579 |
| regulated expression of the feline panleukopenia virus p38 promoter on extrachromosomal fpv/ebv chimeric plasmids. | feline panleukopenia virus/epstein-barr virus (fpv/ebv) chimeric expression plasmids were constructed to study regulation of the structural protein gene of the parvovirus, fpv, in a homologous cell culture system. detection and quantitation of activity from the native fpv promoter, p38, was facilitated by fusing the escherichia coli lacz gene with the fpv structural protein gene. feline cell lines which stably maintained these plasmids extrachromosomally were established. constitutive beta-galac ... | 1989 | 2542586 |