Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effects of endocrine disrupters on sex steroid synthesis and metabolism pathways in fish. | the interactions of estrogenic (nonylphenol, dicofol, atrazine), androgenic (organotins, phthalates, fenarimol) and anti-androgenic compounds (vinclozolin, diuron, p,p'-dde) with key enzymatic activities involved in both synthesis and metabolism of sex hormones was investigated. carp testicular microsomes incubated in the presence of androstenedione and different xenobiotics evidenced higher sensitivity of 5alpha-reductase activity than 17beta-hydroxysteroid dehydrogenase activity towards those ... | 2004 | 15698553 |
| ankrd1 specifically binds casq2 in heart extracts and both proteins are co-enriched in piglet cardiac purkinje cells. | it has been suggested that the cardiac ankyrin repeat domain 1 protein (ankrd1), also known as carp, can play a pathophysiological role in the contractile responsiveness of myocardium. here, we study the potential functional roles of ankrd1 by searching for endogenous cardiac proteins that interact preferentially with ankrd1 in the heart-tissue extract from neonatal piglets, using non-biased pull-down approaches. these approaches identified, for the first time, a selective interaction between an ... | 2005 | 15698842 |
| epitheliocystis in carp (cyprinus carpio) in south korea. | epitheliocystis in the carp of a pet fish market were investigated by our diagnostic work and collecting information from department of laboratory animal medicine and fish & shellfish laboratory. the epitheliocystis was identified by using histopathological examination. epitheliocystis was confirmed as inflammation, epithelial hyperplasia, and lamellar fusion of the gill tissue. electron microscopic observation showed that the inclusions were filled with chlamydia-like organism. | 2005 | 15699608 |
| novel immunoglobulin-like transcripts in teleost fish encode polymorphic receptors with cytoplasmic itam or itim and a new structural ig domain similar to the natural cytotoxicity receptor nkp44. | members of the immunoglobulin superfamily (igsf) include a group of innate immune receptors located in the leukocyte receptor complex (lrc) and other small clusters such as the trem/nkp44 cluster. these receptors are characterised by the presence of immunoglobulin domains, a stalk, a transmembrane domain, and a cytoplasmic region containing either an immunoreceptor tyrosine-based inhibitory motif (itim) or are linked to an adapter molecule with an activation motif (itam) for downstream signallin ... | 2005 | 15702329 |
| the life cycle of henneguya nuesslini schuberg & schroder, 1905 (myxozoa) involves a triactinomyxon-type actinospore. | the life cycle of the histozoic myxozoan parasite henneguya nuesslini was investigated in two salmonid host species. naive brown trout, salmo trutta, and brook trout, salvelinus fontinalis, were experimentally infected in two trials by triactinomyxon type actinospores from naturally infected tubifex tubifex. in exposed common carp, cyprinus carpio, no myxospore production was detected. the parasite formed cysts with mature myxospores in the connective tissue of the fish 102 days post-exposure. t ... | 2005 | 15705152 |
| genetic relationships among members of the ichthyobodo necator complex: implications for the management of aquaculture stocks. | abstract ichthyobodo necator (costia) is a common and important flagellate parasite that infests the skin and gills of many freshwater and marine fish. costia infestations are often fatal and cause significant aquaculture losses worldwide. recently it has been demonstrated that ichthyobodo is a multispecies complex with differing host preferences. knowing if those species have broad or narrow host specificity has important implications for the management of costia. to address the question of hos ... | 2005 | 15705156 |
| ca2+-permeable and ca2+-impermeable ampa receptors coexist on horizontal cells. | fura-2 fluorescent calcium imaging was used for analyzing the subtype of ampa receptors in freshly dissociated horizontal cells of carp retina. exogenous application of ampa induced an increase of intracellular concentration of free ca2+ ([ca2+]i) in horizontal cells, while the [ca2+]i increase was partly inhibited by nifedipine. the residual [ca2+]i increase was completely eliminated by joro spider toxin-3, a blocker of ca2+-permeable ampa receptors. on the other hand, the application of pentob ... | 2005 | 15706232 |
| molecular cloning of prepro-thyrotropin-releasing hormone cdnas from the common carp cyprinus carpio and goldfish carassius auratus. | to expand our knowledge on the evolution of prepro-thyrotropin-releasing hormone (pptrh) from fish to tetrapods, sequences of pptrh cdnas from two cyprinid teleosts, the common carp cyprinus carpio and goldfish carassius auratus, were determined. degenerate primers were designed based on the conserved regions between the zebrafish pptrh sequence identified from the zebrafish est database and the sockeye salmon pptrh sequence, and pcr amplification was performed. full-length pptrhs were confirmed ... | 2005 | 15707606 |
| immunoanalysis of antiviral mx protein expression in japanese flounder (paralichthys olivaceus) cells. | mx proteins are interferon-inducible gtpases that possess antiviral properties in vertebrates. japanese flounder (paralichthys olivaceus) mx protein has previously been shown to possess some antiviral activity against rhabdoviruses. a polyclonal antibody was generated against a purified peptide fragment of japanese flounder mx protein that had been produced in an escherichia coli expression system. the pab detected the approximately 71 kda mx protein from japanese flounder (hirame) natural embry ... | 2004 | 15707665 |
| determination of select antidepressants in fish from an effluent-dominated stream. | increasing evidence indicates widespread occurrence of pharmaceuticals and personal care products (ppcps) in municipal effluent discharges and surface waters. studies that characterize the fate and effects of ppcps in aquatic systems are limited, and to our knowledge, data regarding pharmaceutical accumulation in fish of effluent-dominated ecosystems have not been previously reported. in the present study, fish populations were sampled from a reference stream and an effluent-dominated stream in ... | 2005 | 15720009 |
| systemic priming of alloreactive cytotoxic cells in carp, following anal administration of allogeneic cell antigens. | in the present study, we confirmed that cellular immune responses, especially specific cell-mediated cytotoxicity, could be induced in systemic carp leucocytes, following anal administration of antigens. effector cells isolated from systemic lymphoid tissues (head kidney, spleen and peripheral blood) of carp that were immunised anally with allogeneic cells (epc or kg cell line) efficiently lysed immunogenic target cells. the lytic activity was increased as a result of secondary sensitisation and ... | 2005 | 15722230 |
| seasonal modulation of growth hormone mrna and protein levels in carp pituitary: evidence for two expressed genes. | adaptation of eurythermal fish to naturally varying environmental conditions involves modulation of expressions of various factors in the hypothalamo-hypophyseal axis. here we used three complementary approaches to assess the seasonal variation of growth hormone (gh) protein and mrna levels in pituitary glands of acclimatized carp fish. first, a polyclonal antibody raised against an oligopeptide derived from the carp gh sequence was used for immunohistochemistry; second, oligonucleotides specifi ... | 2005 | 15731922 |
| ultrasonographic findings of facial dysmorphism in wolf-hirschhorn syndrome. | wolf-hirschhorn syndrome has characteristic craniofacial dysmorphism with severe fetal growth restriction. we present a case that revealed facial dysmorphism characteristic of wolf-hirschhorn syndrome with two- and three-dimensional ultrasonography. the facial dysmorphic features in the present case were dolichocephaly; high forehead; large rectangular nose continuing to the eyebrows; hypertelorism; short philtrum; carp-shaped mouth; microretrognathia; and large, simply modeled, low-set ears. ch ... | 2005 | 15731989 |
| effects of short- and long-term ultraviolet b irradiation on the immune system of the common carp (cyprinus carpio). | carp (cyprinus carpio) were repeatedly exposed to 0, 60, 120 and 240 mj/cm2 ultraviolet b (uvb) radiation three times in 1 week (short-term exposure) or 12 times in 4 weeks (long-term exposure). the effect of uvb on the functioning of the carp immune system was studied on day 2 after the final irradiation. after short-term uvb exposure, the whole-blood respiratory burst and cytotoxic activity were markedly enhanced, with parallel responses in both the number of circulating granulocytes and in th ... | 2013 | 15745420 |
| cdna cloning, sequence analysis and expression of 3-methylcholanthrene-inducible cytochrome p450 1b1 in carp (cyprinus carpio). | cytochrome p450 (cyp) genes, which make up a large gene superfamily, are known to play an important role in drug metabolism. the levels of expression of cyp genes in the tissue of fish inhabiting polluted areas have been used extensively in biomonitoring studies as indicators of dioxin pollution. complementary dna of cytochrome cyp1b1 was isolated from carp (cyprinus carpio) liver 24 h after the injection of 3-methylcholanthrene (3-mc). the full length cdna obtained contained a 5' noncoding regi ... | 2004 | 15746899 |
| ontogeny of gnrh-like immunoreactive neuronal systems in the forebrain of the indian major carp, cirrhinus mrigala. | gnrh immunoreactivity appeared in the medial olfactory placode very early in the development of cirrhinus mrigala. the immunoreactive elements were divisible into distinct migratory and non-migratory components. the migratory component appeared as a patch of intensely immunoreactive cells located close to the olfactory epithelium in day 6 post-fertilization larvae. subsequently, these neurons migrate caudally along the ventromedial aspect of the developing forebrain and enroute give rise to gnrh ... | 2005 | 15748717 |
| temperature sensitivity of calcium binding for parvalbumins from antarctic and temperate zone teleost fishes. | parvalbumin (pv) is a soluble calcium-binding protein that is especially abundant in fast-twitch muscles of fish and other lower vertebrates. despite its prevalence in ectothermic taxa, few data address the effects of temperature on pv binding function. in this study, calcium dissociation constants (kd) were measured as a function of temperature (0-25 degrees c) for pv from two antarctic (gobionotothen gibberifrons and chaenocephalus aceratus) and two temperate zone fish species (cyprinus carpio ... | 2005 | 15748857 |
| isolation of cdna of novel cytochrome p450 1b gene, cyp1b2, from carp (cyprinus carpio) and its induced expression in gills. | cytochrome p450 (cyp) enzymes constitute a multigene family of many endogenous and xenobiotic substances. the cyp1 family is of particular interest in environmental toxicology because its members are dominant in the metabolism of polycyclic aromatic hydrocarbons (pahs), polychlorinated biphenyls (pcbs), and aryl amines. a new complementary dna of the cyp1b subfamily encoding cyp1b2 was isolated from carp liver after intraperitoneal injection with 3-methylcholanthrene (3-mc). the obtained full-le ... | 2004 | 15750580 |
| long-term potentiation and olfactory memory formation in the carp (cyprinus carpio l.) olfactory bulb. | long-term potentiation of synaptic transmission is considered to be an elementary process underlying the cellular mechanism of memory formation. in the present study we aimed to examine whether or not the dendrodendritic mitral-to-granule cell synapses in the carp olfactory bulb show plastic changes after their repeated activation. it was found that: (1) the dendrodendritic mitral-to-granule cell synapses showed three types of plasticity after tetanic electrical stimulation applied to the olfact ... | 2005 | 15750817 |
| differential spontaneous killing of human and murine tumour cell lines by leucocyte subpopulations from carp peripheral blood leucocytes. | cell populations from carp (cyprinus carpio l.) peripheral blood leucocytes (pbls) were examined for nonspecific cytotoxicities. by using monoclonal antibodies (mabs) against carp thrombocytes (tcl-hb8) and both neutrophils and monocytes (tcl-be8), pbls with a density of 1.08 g ml-1 were separated into three fractions: thrombocytes, a mixture of neutrophils and monocytes, and other cells (mainly lymphocytes), and the separated cells were tested for cytotoxic activities against mammalian tumour c ... | 2005 | 15752650 |
| sandwich immunoassay for determination of vitellogenin in golden grey mullet (liza aurata) serum as a field exposure biomarker. | vitellogenin (vtg) is a protein produced in the liver of oviparous animals in response to oestrogens. abnormal production of vtg by males, therefore, is used as a biological indicator of exposure to xeno-oestrogens. in this study, a sandwich-elisa for measuring vtg in liza aurata (golden grey mullet) was developed and validated. plasma vtg was purified from 17beta-oestradiol-injected immature individuals of mullet, by size-exclusion and ion-exchange chromatography. polyclonal antibodies against ... | 2005 | 15756602 |
| [evidence of the unreduced diploid eggs generated from the diploid gynogenetic progeny of allotetraploid hybrids]. | following activation by uv-irradiated sperms from scatter scale common carp and without the treatment for doubling the chromosome number, the eggs generated by the diploid gynogenetic progeny (g1) with 100 chromosomes,developed into diploid gynogenetic progeny (g2) with 100 chromosomes. both the males and females of the tetraploids with 200 chromosomes were obtained from the offsprings (g1 x at) produced by mating the eggs of g1 with the diploid sperms from the allotetraploid hybrids. the result ... | 2005 | 15759860 |
| [phylogeny of chinese catfishes inferred from mitochondrial cytochrome b sequences]. | the mitochondrial dna cytochrome b gene was sequenced from 27 catfish species representing 11 families and 24 genera catfishes in china. aligning with cytochrome b sequences of eight catfish species from north america and africa retrieved from genbank, and selecting astyanax mexicanu, cyprinus carpio, and sardinops melanostictus as outgroups, we constructed a matrix of 38 dna sequences. the phylogenetic trees were constructed by using bayesian method and maximum parsimony (mp) method. the result ... | 2005 | 15759861 |
| temperature alters the respiratory surface area of crucian carp carassius carassius and goldfish carassius auratus. | we have previously found that the gills of crucian carp carassius carassius living in normoxic (aerated) water lack protruding lamellae, the primary site of o(2) uptake in fish, and that exposing them to hypoxia increases the respiratory surface area of the gills approximately 7.5-fold. we here examine whether this morphological change is triggered by temperature. we acclimated crucian carp to 10, 15, 20 and 25 degrees c for 1 month, and investigated gill morphology, oxygen consumption and the c ... | 2005 | 15767311 |
| [effect of furosemide on gabaa-induced 36cl transport on atpase activity in synaptic membranes of carp (cyprinus carpio l.)]. | we studied the effect of furosemide on gabaa-induced 36cl transport and gabaa-induced cl- -atpase activity in synaptic membranes of fish brain. at physiological ph of 7.4, gaba (0.1-100 microm) stimulated 36cl transport in synaptoneurosomes and cl- -atpase activity in synaptic membranes. furosemide (0.1-0.5 mm) removed the activating effect of the mediator on chlorine transport and enzyme activity (i50 equaled 0.16 and 0.12 mm, respectively). in the absence of the mediator, picrotoxin (50 microm ... | 2016 | 15768629 |
| uniquely stable 40 kda subfragment-2 in carp myosin. | digestion of carp myofibrils at 30 degrees c in 0.5 m kcl medium with calcium ion generated unique 135 kda heavy meromyosin (hmm). the hmm was not produced when digested at 10 degrees c. a further digestion of the 135 kd hmm isolated in the absence of calcium ion generated uniquely short subfragment-2 (s-2) with a size of 40 kda (40 kda s-2) together with subfragment-1 (s-1). the 40 kda s-2 was identified by n- and c-end sequencing, and demonstrated to locate the amino end of the rod portion. th ... | 2005 | 15769163 |
| [genetic structure of crucian carp (cypriniformes, cyprinidae, carassius l. 1758) populations of middle-dnieper basin]. | comparative analysis of middle dnieper crucian carp's population structure was made by means of biochemical gene marking, cytometry and biological analysis. as a result the structure was found, which may by expounded like association of the genetically related species and forms. this association consists of diploid c. auratus (which predominates in population), c. carassius (which was found in the some of north-eastern reservoirs only) and triploid c. gibelio. in ukraine the homogeneous populati ... | 2008 | 15771088 |
| cloning of the koi herpesvirus (khv) gene encoding thymidine kinase and its use for a highly sensitive pcr based diagnosis. | outbreaks with mass mortality among common carp cyprinus carpio carpio and koi cyprinus carpio koi have occurred worldwide since 1998. the herpes-like virus isolated from diseased fish is different from herpesvirus cyprini and channel catfish virus and was accordingly designated koi herpesvirus (khv). diagnosis of khv infection based on viral isolation and current pcr assays has a limited sensitivity and therefore new tools for the diagnosis of khv infections are necessary. | 2005 | 15774009 |
| recently published papers: from superbugs to superbowl. | several papers are discussed including the carp (coronary artery revascularisation prophylaxis) trial, examining the role, if any, of preoperative coronary revascularisation and a study examining the heart rate in the intensive care unit (icu) population. we also take a look at infections in the icu with particular regard to a recent study which examined isolating patients with methicillin-resistant staphylococcus aureus (mrsa). | 2005 | 15774060 |
| [anatomoradiological correlation of the hypothenar hammer syndrome]. | hypothenar hammer syndrome is all the clinical ischemic secondary manifestations in a stenose even one occlusion of traumatic origin of the distal portion of the ulnar artery. our objective was to underline anatomical substratum of this lesion and to emphasize the interest of the angio m.r.i. in the diagnosis of this syndrome. the radioanatomical study of the distal portion of the ulnar artery were on 10 fresh corpses of both sexes free of traumatic hurt of upperlimb. on 6 anatomical details inj ... | 2003 | 15776638 |
| the complete genomic sequence of the carp fast skeletal myosin heavy chain gene. | we have determined the complete dna nucleotide sequence of the carp cyprinus carpio fast skeletal myosin heavy chain (myh) gene. introns and exons were predicted by comparison with the corresponding carp myh cdnas previously reported. the gene encoded the entire mrna transcript and contained 5958 nucleotides (nt) including 77 nt 5'-untranslated region, 5796 nt coding region for 1931 amino acids, and 85 nt 3'-untranslated region. the coding region was split by 38 introns and the complete gene con ... | 2005 | 15777658 |
| hypoxia and recovery perturb free radical processes and antioxidant potential in common carp (cyprinus carpio) tissues. | the effects of hypoxia exposure and subsequent normoxic recovery on the levels of lipid peroxides (looh), thiobarbituric acid reactive substances (tbars), carbonylproteins, total glutathione levels, and the activities of six antioxidant enzymes were measured in brain, liver, kidney and skeletal muscle of the common carp cyprinus carpio. hypoxia exposure (25% of normal oxygen level) for 5h generally decreased the levels of oxidative damage products, but in liver tbars content were elevated. hypox ... | 2005 | 15778094 |
| undetectable apolipoprotein a-i gene expression suggests an unusual mechanism of dietary lipid mobilisation in the intestine of cyprinus carpio. | high density lipoprotein (hdl) has been shown to play an important role in the dietary lipid mobilisation in the carp. in spite of this, previous studies have failed to demonstrate the synthesis of the major protein component of hdl, apolipoprotein a-i (apoa-i), in the proximal intestine of the carp. therefore, the aim of the present study was to evaluate the expression of apoa-i throughout the entire intestine. curiously, no transcription of the apoa-i gene could be detected either by northern ... | 2005 | 15781899 |
| uptake and enantioselective elimination of chlordane compounds by common carp (cyprinus carpio, l.). | an analytical method involving supercritical fluid extraction (sfe) followed by a two-dimensional gas chromatography (2d-gc) analysis was developed to determine the concentration (first gc) and enantiomeric ratio (second gc) of cis- and trans-chlordanes at the ppb (ng/g) level in fish tissue. the sfe method allowed concentration of the compounds of interest, and reduced the number of extraction and sample clean-up manipulations as compared to classical solvent extraction techniques. four hundred ... | 2005 | 15788172 |
| lessons from trail-resistance mechanisms in colorectal cancer cells: paving the road to patient-tailored therapy. | colorectal cancer is one of the leading causes of cancer-related deaths worldwide. intrinsic, as well as acquired, resistance to chemotherapy remains a major problem in the treatment of this disease. it is, therefore, of great importance to develop new, patient-tailored, treatment strategies for colorectal cancer patients. tumor necrosis factor-related apoptosis-inducing ligand (trail) acts through the pro-apoptotic dr4 and dr5 receptors in tumor cells without harming normal cells and will soon ... | 2004 | 15790545 |
| a survey of potential stressor-induced physiological changes in carp (cyprinus carpio) and barbel (barbus bocagei) along the tajo river. | the objective of this study was to evaluate fish response to acute stress induced by confinement after capture. because of the previously reported presence of chemical compounds in the tajo river basin where the study samplinig took place, an exposure biomarker to organic chemicals (retinol) was used. cortisol and glucose were used as stress biomarkers. plasma levels of cholesterol were used as lipidic metabolism indicators, and retinol level was used as a specific exposure biomarker. a referenc ... | 2005 | 15793831 |
| calcium-permeable ampa receptor plasticity is mediated by subunit-specific interactions with pick1 and nsf. | a recently described form of synaptic plasticity results in dynamic changes in the calcium permeability of synaptic ampa receptors. since the ampa receptor glur2 subunit confers calcium permeability, this plasticity is thought to occur through the dynamic exchange of synaptic glur2-lacking and glur2-containing receptors. to investigate the molecular mechanisms underlying this calcium-permeable ampa receptor plasticity (carp), we examined whether ampa receptor exchange was mediated by subunit-spe ... | 2005 | 15797551 |
| disruption of the gene euchromatin histone methyl transferase1 (eu-hmtase1) is associated with the 9q34 subtelomeric deletion syndrome. | a new syndrome has been recognised following thorough analysis of patients with a terminal submicroscopic subtelomeric deletion of chromosome 9q. these have in common severe mental retardation, hypotonia, brachycephaly, flat face with hypertelorism, synophrys, anteverted nares, thickened lower lip, carp mouth with macroglossia, and conotruncal heart defects. the minimum critical region responsible for this 9q subtelomeric deletion syndrome (9q-) is approximately 1.2 mb and encompasses at least 1 ... | 2005 | 15805155 |
| identification of the genes involved in enhanced fenretinide-induced apoptosis by parthenolide in human hepatoma cells. | fenretinide (n-4-hydroxyphenyl retinamide, 4hpr) is a synthetic anticancer retinoid that is a well-known apoptosis-inducing agent. recently, we observed that the apoptosis induced by fenretinide could be effectively enhanced in hepatoma cells by a concomitant treatment with parthenolide, which is a known inhibitor of nuclear factor-kappab (nf-kappab). furthermore, treatment with fenretinide triggered the activation of nf-kappab during apoptosis, which could be substantially inhibited by partheno ... | 2005 | 15805281 |
| expression profiling with progression of dystrophic change in dysferlin-deficient mice (sjl). | the sjl mouse is a model for human dysferlinopathy (limb-girdle muscular dystrophy type 2b and miyoshi myopathy). we used cdna microarrays to compare the expression profiles of 10,012 genes in control and sjl quadriceps femoris muscles in order to find genes involved in the degeneration and regeneration process and in dysferlin's functional network. many genes involved in the process of muscle regeneration are observed to be up-regulated in sjl mice, including cardiac ankyrin repeated protein (c ... | 2005 | 15811552 |
| the immunomodulatory effects of the dimer of lysozyme (klp-602) in carp (cyprinus carpio l)--in vivo study. | the dimer of lysozyme was administered to carp (cyprinus carpio l.) in an attempt to reduce the suppression of response in fish exposed to the cypermethrin. experimental studies were carried out on cells isolated from blood and pronephros of carp. in the in vivo studies fish were intoxicated with cypermethrin (synthetic pesticide) at a dose of ld5. after 24 h fish were injected intraperitoneally with a modulator-the dimer of lysozyme (klp-602) at a dose of 0.02 mg/kg of body weight. in the study ... | 2005 | 15814318 |
| characterisation of t cell antigen receptor alpha chain isotypes in the common carp. | t cell receptor alpha (tcralpha) chain has been characterised in several teleost species to date. here, a reverse transcription-polymerase chain reaction (rt-pcr) strategy was used to isolate cdna clones encoding tcralpha chain from an individual of the common carp (cyprinus carpio.l.). the valpha sequences identified were most similar to valpha of other teleosts, and could be classified into as many as 14 valpha families. for the jalpha sequences, diversity comparable to that seen in other tele ... | 2005 | 15820122 |
| caspase-dependent processing activates the proapoptotic activity of deleted in breast cancer-1 during tumor necrosis factor-alpha-mediated death signaling. | deleted in breast cancer-1 (dbc-1) was initially cloned from a homozygously deleted region in breast and other cancers on human chromosome 8p21, although no function is known for the protein product it encodes. we identified the generation of amino-terminally truncated versions of dbc-1 during tumor necrosis factor (tnf)-alpha-mediated apoptosis. full-length 150 kda dbc-1 underwent caspase-dependent processing during tnf-alpha-mediated death signaling, to produce p120 dbc-1 and p66 dbc-1 carboxy ... | 2005 | 15824730 |
| involvement of gadd153 and cardiac ankyrin repeat protein in hypoxia-induced apoptosis of h9c2 cells. | oxidative stress is the main cause of cardiac injury during ischemia/reperfusion but the molecular mechanism for this process is unclear. in this study, it was found that hypoxia induces apoptosis in rat embryonic heart-derived h9c2 cells leading to the induction of gadd153, which is an apoptosis-related gene. therefore, this study addressed the molecular role of gadd153 in hypoxia-induced apoptosis. the stable or inducible overexpression of gadd153 sensitized the h9c2 cells to apoptotic cell de ... | 2005 | 15826945 |
| plasma vitellogenin levels and gonadal morphology of wild carp (cyprinus carpio l.) in a receiving rivers downstream of sewage treatment plants. | a field study was carried out in order to assess, in a wild cyprinid fish, the biological effects related to the exposure to estrogenic compounds. the gonadosomic index (gsi), plasma vitellogenin (vtg) level and histological observations of gonads were performed to evaluate the reproductive health in common carp (cyprinus carpio) from three rivers receiving sewage treatment plant (stp) effluents (guadarrama, henares and jarama rivers, spain) and from a reference site (lozoya river). no significa ... | 2004 | 15833242 |
| importance of photoperiods in the regulation of ovarian activities in indian major carp catla catla in an annual cycle. | the present study attempted for the first time to explore the importance of photoperiod in the regulation of seasonal ovarian functions in any subtropical major carp. adult indian major carp catla catla were transferred to a long photoperiod (lp; ld 16:8) or a short photoperiod (sp; ld 8:16) for 30 days on 4 dates corresponding to the beginnings of 4 reproductive phases in an annual cycle, and responsiveness of the ovary was evaluated by comparison with the gonadal weight (i(g)), relative number ... | 2005 | 15834111 |
| protection of cultured cyprinus carpio against a lethal viral disease by an attenuated virus vaccine. | massive mortality of koi and common carp--cyprinus carpio species--has been observed since 1998 in many countries worldwide, resulting in severe economic losses. the cause of the disease is an as yet unclassified large dna virus, designated carp nephritis gill necrosis virus (cngv) or koi herpes virus (khv). previously, we demonstrated that the wild type cngv lost its pathogenecity following serial transfer in cell culture, and that clones isolated from the attenuated population can be used as a ... | 2005 | 15837363 |
| [construction and characteristics analysis of somatic cell lines from common carp cyprinus carpio]. | epithelia of rostral side and fins from common carp were taken to culture in vitro. as a result, cells of tail fins were successfully passed from generation to generation, and constructed cell lines. during the culture procedure, the passage cells were observed and their biological characteristics were analyzed, which consist of morphologic analysis, drawing of growth curve, detection of producing rate for clones, insight of adaptation to temperatures, calculation of chromosomes and investigatio ... | 2005 | 15839211 |
| first ovarian maturity and recovery response in common carp, cyprinus carpio after exposure to carbofuran. | the present study embodies the observations on duration (45, 75 and 105 days) dependent effects of toxicologically low concentration (16 ppm) of a carbamate pesticide, carbofuran, on first ovarian maturity with correlative recovery response by withdrawal of treatment for 30 days in virgin yearlings of common carp, cyprinus carpio var. communis. in control fish histological organization of ovary and changes in gonado-somatic index (gsi) revealed the initiation of oocyte differentiation and develo ... | 2004 | 15847330 |
| gill lesions in the major carp, labeo rohita exposed to lethal and sublethal concentrations of tannery effluent. | the major carp, labeo rohita were exposed to (0.873%) lethal and sublethal (0.073%) concentrations of tannery effluent for 24h and 40 days respectively under static bioassay condition. the surface architecture of gill revealed severe damages such as : fusion and clumping in the middle and distal parts of the primary lamellae, swelling and deterioration of the cells. the interlamellar space was filled either with hyperplastic epithelial or mucous cells. secondary lamellae lost their identity and ... | 2004 | 15847345 |
| molecular cloning and characterization of two types of cd8alpha from ginbuna crucian carp, carassius auratus langsdorfii. | we cloned and sequenced full-length cdnas for two types of cd8alpha from the s3n strain of ginbuna crucian carp (carassius auratus langsdorfii) and quantified the expression of cd8alpha genes after sensitization by scale grafting, employing a model system of clonal triploid ginbuna and tetraploid ginbuna-goldfish hybrids. rt-pcr yielded four different fragments of cd8alpha homologue from the s3n strain of ginbuna and these sequences were classified into two groups. the two types of ginbuna cd8al ... | 2005 | 15854681 |
| quantitative structure-activity relationships for the toxicity of substituted benzenes to cyprinus carpio. | to measure the 96h-lc50 values of 32 substituted benzenes to the carp and to study the relationship between quantitative structure-activity and structural parameters of chemicals. | 2005 | 15861779 |
| administration of yeast glucan enhances survival and some non-specific and specific immune parameters in carp (cyprinus carpio) infected with aeromonas hydrophila. | effects of beta-glucan administration on survival and immune modulations were studied in cyprinus carpio against the bacterial pathogen, aeromonas hydrophila. beta-glucan was extracted from saccharomyces cervisiae and purified. a virulent strain of the pathogen a. hydrophila was collected from infected fish. different concentrations of beta-glucan were administered to test animals on day 1, 3 and 5 through different routes (intraperitoneal injection (ip), bathing and oral administration). contro ... | 2005 | 15863011 |
| comparison of respiratory burst activity of inflammatory neutrophils in ayu (plecoglossus altivelis) and carp (cyprinus carpio). | neutrophils of ayu (plecoglossus altivelis) were previously shown to have unusually high respiratory burst activity (rba). to understand this unique character of ayu neutrophils, the rbas of resting and inflammatory neutrophils of ayu and carp (cyprinus carpio) were compared. inflammation was induced in the peritoneal cavity by injecting killed-bacteria. the rba of peritoneal-exudate (inflammatory) neutrophils was measured after stimulation with phorbol myristate acetate (pma). resting neutrophi ... | 2005 | 15863016 |
| transferrin and antiproteases are major proteins of common carp seminal plasma. | 2005 | 15863018 | |
| the effects of 3-methylcholanthrene on lymphocyte proliferation in the common carp (cyprinus carpio l.). | the sensitivity of lymphocyte proliferation as bioindicator of pollution stress was evaluated in the common carp (cyrinus carpio l.). the time course response of peripheral blood leukocyte proliferation in response or not to mitogens was measured from 1 to 7 days after peritoneal injection of 3-methylcholantrene (3-mc), and compared to the time course response of a highly sensitive biomarker, induction of cytochrome p450. 3-methylcholanthrene (40 mg kg(-1)) inhibited both b- and t-lymphocyte pro ... | 2005 | 15863259 |
| expression of endogenous and exogenous growth hormone (gh) messenger (m) rna in a gh-transgenic tilapia (oreochromis niloticus). | we have previously produced transgenic fish from crosses between a wild-type female tilapia (oreochromis niloticus) and a g transgenic male. this line of growth-enhanced tilapia carries a single copy of a chinook salmon (s) growth hormone (gh) gene spliced to an ocean pout antifreeze promoter (opa-fpcsgh) co-ligated to a carp beta-actin/lacz reporter gene construct, integrated into the tilapia genome. because little is known about the expression sites of transgenes, we have characterised the gen ... | 2005 | 15865052 |
| nitric oxide release is induced by dopamine during illumination of the carp retina: serial neurochemical control of light adaptation. | several lines of indirect evidence have suggested that nitric oxide may play an important role during light adaptation of the vertebrate retina. we aimed to verify directly the effect of light on nitric oxide release in the isolated carp retina and to investigate the relationship between nitric oxide and dopamine, an established neuromodulator of retinal light adaptation. using a biochemical nitric oxide assay, we found that steady or flicker light stimulation enhanced retinal nitric oxide produ ... | 2005 | 15869516 |
| effects of redox cycling compounds on dt diaphorase activity in the liver of rainbow trout (oncorhynchus mykiss). | background: dt diaphorase (dtd; nad(p)h:quinone oxidoreductase; ec 1.6.99.2) catalyses the two electron reduction of quinones, thus preventing redox cycling and consequently quinone dependent production of reactive oxygen species. in rat and mouse, a wide range of chemicals including polyaromatic hydrocarbons, azo dyes and quinones induces dtd. bifunctional compounds, such as beta-naphthoflavone (beta-nf) and benzo(a)pyrene (b(a)p), induce dtd together with cyp1a and phase ii enzymes by a mechan ... | 2005 | 15871734 |
| four types of smad4 found in the common carp, cyprinus carpio. | smad4 is defined as the common-mediator smad (co-smad) required for transducing signals for all transforming growth factor-beta (tgf-beta) superfamily members. in this study, we have isolated eight distinct smad4 full-length cdnas from the common carp (cyprinus carpio). these cdnas were classified into four types and each type consisted of two subtypes. the eight cdnas encoded four distinct proteins ranging from 505aa to 568aa in size, with close similarities in the mad homology 1 and 2 (mh1 and ... | 2005 | 15880772 |
| perfluorinated compounds in aquatic organisms at various trophic levels in a great lakes food chain. | trophic transfer of perfluorooctanesulfonate (pfos) and other related perfluorinated compounds was examined in a great lakes benthic foodweb including water-algae-zebra mussel-round goby-smallmouth bass. in addition, perfluorinated compounds were measured in livers and eggs of chinook salmon and lake whitefish, in muscle tissue of carp, and in eggs of brown trout collected from michigan. similarly, green frog livers, snapping turtle plasma, mink livers, and bald eagle tissues were analyzed to de ... | 2005 | 15883668 |
| uncoupling protein 1 in fish uncovers an ancient evolutionary history of mammalian nonshivering thermogenesis. | uncoupling proteins (ucps) increase proton leakage across the inner mitochondrial membrane. thereby, ucp1 in brown adipose tissue dissipates proton motive force as heat. this mechanism of nonshivering thermogenesis is considered as a monophyletic trait of endothermic placental mammals that emerged about 140 million years ago and provided a crucial advantage for life in the cold. the paralogues ucp2 and ucp3 are probably not thermogenic proteins but convey mild uncoupling, which may serve to redu ... | 2005 | 15886331 |
| concentrations of arsenic, cadmium, copper, lead, selenium, and zinc in fish from the mississippi river basin, 1995. | fish were collected in late 1995 from 34 national contaminant biomonitoring program (ncbp) stations and 12 national water quality assessment program (nawqa) stations in the mississippi river basin (mrb), and in late 1996 from a reference site in west virginia. the ncbp sites represented key points (dams, tributaries, etc.) in the largest rivers of the mrb. the nawqa sites were typically on smaller rivers and were selected to represent dominant land uses in their watersheds. the west virginia sit ... | 2004 | 15887377 |
| phylogeny and ontogeny of fish leucocytes. | in contrast to higher vertebrates, most fish species hatch at the embryonic stage of life. consequently, they have to defend against a variety of micro-organisms living in their aquatic environment. this paper is focussed on the development of leucocytes functioning within this early innate system and later on in the acquired immune system (b and t cells). most of the data are derived from cyprinid fish (zebrafish, carp), which are excellent models to study early ontogeny. attention is also paid ... | 2005 | 15890532 |
| acth, alpha-msh, and control of cortisol release: cloning, sequencing, and functional expression of the melanocortin-2 and melanocortin-5 receptor in cyprinus carpio. | cortisol release from fish head kidney during the acute phase of the stress response is controlled by the adrenocorticotropic hormone (acth) from the pituitary pars distalis (pd). alpha-melanocyte-stimulating hormone (alpha-msh) and beta-endorphin, from the pars intermedia (pi), have been implicated in cortisol release during the chronic phase. the present study addresses the regulation of cortisol release by acth and alpha-msh in common carp (cyprinus carpio) and includes characterization of th ... | 2005 | 15890786 |
| in vivo and in vitro exposures of carp and carp tissues to graded concentrations of endocrine disrupting chemicals. | the objective of this study was to correlate morphological and biochemical parameters of reproductive activity in fish exposed to graded concentrations of endocrine disrupting chemicals. two-year-old carps were exposed for two weeks to graded concentrations of ethynyl-estradiol, tamoxifen, and flutamide. for each chemical, morphological alterations of the gonads and liver were described according to biomarker and sex steroid levels. in parallel, isolated follicular cells (fcs) and testis fragmen ... | 2005 | 15891032 |
| regulation of the stress response in early vertebrates. | the acute stress response is a key regulatory system for the maintenance of homeostatic equilibrium that is activated upon an imminent or ongoing disturbance of the "milieu interieur". in general, the stress response in bony fish is similar to that of mammals. the recent cloning and characterization of corticotropin-releasing hormone-binding protein (crh-bp) in carp (cyprinus carpio l.) reflects the conservation of the crh signaling system throughout vertebrates. now, we can start to investigate ... | 2005 | 15891057 |
| fish community characterization in two stretches upstream and downstream of the lambro river confluence with the po river. | this study aimed to characterize the fish community in two stretches of the po river, upstream and downstream of the lambro river confluence, to verify the environmental effects of the presence of endocrine disrupters (eds). more than 5000 specimens were captured, identified at the species level, and recorded for biometric parameters. plasma, liver, and gonad fragments were sampled for histological and biochemical analyses. few specimens belonging to carp and barbel showed gonads with the concom ... | 2005 | 15891083 |
| cloning and analysis of non-specific cytotoxic cell receptor (nccrp)-1 from common carp cyprinus carpio l. | nonspecific cytotoxic cell receptor protein (nccrp-1) provides an important function in target cell recognition and activation of cytotoxicity. nccrp-1 has been cloned from common carp cyprinus carpio l. from fish barbel by est analysis. the isolated gene is composed of 945 bp with a 79 bp 5' utr, 714 bp open reading frame and 152 bp 3' utr. the predicted nccrp-1 gene is composed of 237 amino acid residues and its predicted signal peptide is 19 amino acid residues in length. this gene has conser ... | 2005 | 15897012 |
| acute toxicity of synthetic pyrethroids to indian major carp, catla catla l. | 2005 | 15903198 | |
| effects of bisphenol a-related diphenylalkanes on vitellogenin production in male carp (cyprinus carpio) hepatocytes and aromatase (cyp19) activity in human h295r adrenocortical carcinoma cells. | the present study investigated the effects of the known xenoestrogen bisphenol a (bpa) relative to eight bpa-related diphenylalkanes on estrogen receptor (er)-mediated vitellogenin (vtg) production in hepatocytes from male carp (cyprinus carpio), and on aromatase (cyp19) activity in the human adrenocortical h295r carcinoma cell line. of the eight diphenylalkanes, only 4,4'-(hexafluoropropylidene)diphenol (bhf) and 2,2'-bis(4-hydroxy-3-methylphenyl)propane (bpro) induced vtg, i.e., to a maximum o ... | 2005 | 15907334 |
| glycosylation is essential for translocation of carp retinol-binding protein across the endoplasmic reticulum membrane. | retinoid transport is well characterized in many vertebrates, while it is still largely unexplored in fish. to study the transport and utilization of vitamin a in these organisms, we have isolated from a carp liver cdna library retinol-binding protein, its plasma carrier. the primary structure of carp retinol-binding protein is very conserved, but presents unique features compared to those of the correspondent proteins isolated and characterized so far in other species: it has an uncleavable sig ... | 2005 | 15910754 |
| levels of organochlorine pesticides, polychlorinated biphenyls and polybrominated diphenyl ethers in fish species from kahramanmaras, turkey. | the levels of organohalogenated contaminants, such as organochlorine pesticides (ocps), polychlorinated biphenyls (pcbs) and polybrominated diphenyl ethers (pbdes) were measured in four fish species (acanthobrama marmid (kalashpa), cyprinus carpio (carp), chondrostoma regium (nose-carp), and silurus glanis (wels)) from the sir dam lake, kahramanmaras, turkey. these species were selected for their characteristic feeding behaviour and their importance to local human fish consumption. ddts were the ... | 2005 | 15910967 |
| bioaccumulation studies of organochlorinated pesticides in tissues of cyprinus carpio. | freshwater fish cyprinus carpio was selected for the study of bioaccumulation of organochlorinated pesticides in tissues like gills, muscle, intestine, kidney, and liver in a continuous fed system. the pesticides used were aldrin, dieldrin, bhc, and ddt. the bioaccumulation of dieldrin was maximum of 85.0 microg g(-1) wet weight in liver tissue while minimum of 7.30 microg g(-1) wet weight for ddt at 30 days exposure time. bioconcentration factor (bcf) has followed the same trend in liver tissue ... | 2005 | 15913013 |
| evaluation of pesticides and metals in fish of the dniester river, moldova. | the dniester river is one of the largest rivers in eastern europe and receives input from numerous anthropogenic activities. in october 2001, fish samples (abramis brama, rutilus rutilus heckeli, perca fluviatilis, chondrostoma nasus, carassius auratus gibelio, and cyprinus carpio) were collected and analyzed for metals and various pesticides. maximum total ddt pesticide concentrations were between 27.7 and 34.2 ng/g wet weight in muscles and gonads, respectively. dde isomers comprised up to 70% ... | 2005 | 15914239 |
| koi herpesvirus represents a third cyprinid herpesvirus (cyhv-3) in the family herpesviridae. | the sequences of four complete genes were analysed in order to determine the relatedness of koi herpesvirus (khv) to three fish viruses in the family herpesviridae: carp pox herpesvirus (cyprinid herpesvirus 1, cyhv-1), haematopoietic necrosis herpesvirus of goldfish (cyprinid herpesvirus 2, cyhv-2) and channel catfish virus (ictalurid herpesvirus 1, ichv-1). the genes were predicted to encode a helicase, an intercapsomeric triplex protein, the dna polymerase and the major capsid protein. the re ... | 2005 | 15914843 |
| complementary dna cloning and constitutive expression of cytochrome p450 1c1 in the gills of carp (cyprinus carpio). | cytochrome p450 (cyp) enzymes constitute a multigene family of many endogenous and xenobiotic substances. the cyp1 family is of particular interest in environmental toxicology because its members are dominant in the metabolism of polycyclic aromatic hydrocarbons (pahs), polychlorinated biphenyls (pcbs), and aryl amines. a new complementary dna of the cyp1c subfamily encoding cyp1c1 was isolated from carp liver after intraperitoneal injection of beta-napthoflavone (bnf). the full-length cdna obta ... | 2005 | 15915151 |
| in vivo evoked specific cell mediated cytotoxicity in carp (cyprinus carpio l.) uses mainly a perforin/granzyme-like pathway. | a specific cytotoxicity assay has been developed for carp using the epithelioma papulosum cyprini (epc) cell line as target cells and the release of lactate dehydrogenase (ldh) as read out system. non-specific cytotoxicity against epc was not observed, but animals immunized with epc clearly showed specific killing by effector cells present in kidney, blood and spleen. this killing was strongly calcium dependent, suggesting the utilization of a perforin/granzyme-like pathway. however, blocking wi ... | 2006 | 15916904 |
| ontogeny of humoral immune parameters in fish. | the first appearance of igm in lymphocytes varies considerably among fish species. generally, the first appearance of b-lymphocytes and immunoglobulins is late in marine species compared to fresh water species, and larvae have reached about 20-30 mm in length when igm is first expressed. rainbow trout and channel catfish show the first appearances of surface igm at about 1 week after hatching. marine species like the sea bass, spotted wolffish and cod show igm positive lymphocytes 1-10 weeks aft ... | 2005 | 15916905 |
| cell proliferation and gill morphology in anoxic crucian carp. | is dna replication/cell proliferation in vertebrates possible during anoxia? the oxygen dependence of ribonucleotide reductase (rnr) could lead to a stop in dna synthesis, thereby making anoxic dna replication impossible. we have studied this question in an anoxia-tolerant vertebrate, the crucian carp (carassius carassius), by examining 5'-bromo-2'-deoxyuridine incorporation and proliferating cell nuclear antigen levels in the gills, intestinal crypts, and liver. we exposed crucian carp to 1 and ... | 2005 | 15919732 |
| extracellular calcium is required for the maintenance of plasma membrane integrity in nucleated cells. | in contrast to rat and human erythrocytes, nucleated erythrocytes from two fish species (cyprinus carpio and salmo trutta) underwent almost complete haemolysis in 20 min of edta addition. using ca2+/mg2+ egta-citrate buffer, we observed that half-maximal haemolysis of fish erythrocytes occurs at [ca2+]o approximately 10 microm independently of extracellular mg2+ concentration. attenuation of [ca2+]o with egta also decreased stability of the plasma membrane of vascular smooth muscle cells (vsmc) ... | 2005 | 15936814 |
| improvement of nutritive value of grass pea (lathyrus sativus) seed meal in the formulated diets for rohu, labeo rohita (hamilton) fingerlings after fermentation with a fish gut bacterium. | eight isonitrogenous (35% crude protein approximately) and isocaloric (4.0 kcalg(-1) approximately) diets were formulated incorporating raw and fermented grass pea (lathyrus sativus) seed meal at 10%, 20%, 30% and 40% levels by weight into a fish meal based diet and fed to rohu, labeo rohita, fingerlings for 80 days and fish performance was studied. a particular bacterial strain (bacillus sp.) isolated from the intestine of adult common carp (cyprinus carpio) reared in the wild having significan ... | 2005 | 15939274 |
| assessment of heavy metal concentrations in the food web of lake beyşehir, turkey. | the accumulation of heavy metals (cd, pb, hg and cr) was measured by atomic absorption spectrophotometry in water, sediment, plankton and fish samples collected from lake beyşehir, which is important bird nesting and visiting areas, and irrigation and drinking water sources. in lake beyşehir, the accumulation orders of heavy metals were cd>pb>cr>hg in water, pb>cd>cr>hg in sediment, pb>cd>cr>hg in plankton, and cd>pb>cr>hg in the muscles and gills of chub, carp, tench, except for the muscle of p ... | 2005 | 15950047 |
| constitutive expression of three novel catfish cxc chemokines: homeostatic chemokines in teleost fish. | chemokines are best known for their vital role in leukocyte chemotaxis, as part of the larger inflammatory response. expression analysis and functional characterization of chemokines in mammalian species have often overlooked the role of these proteins under homeostatic conditions. recent investigations of chemokine diversity in teleost fish have also centered on the immune-related functions of chemotactic cytokines, such as cxcl8 and cxcl10. while a disease-based approach to chemokines is essen ... | 2005 | 15950731 |
| congenital heart disease reminiscent of partial trisomy 2p syndrome in mice transgenic for the transcription factor lbh. | partial trisomy 2p syndrome includes a spectrum of congenital heart disease (chd) that is characterized by complex malformations of the outflow and inflow tracts, defects in cardiac septation, heart position, as well as abnormal ventricular development. lbh (limb-bud and heart) is a novel, highly conserved putative transcriptional regulatory protein, which displays a unique spatiotemporal gene expression pattern during early mouse heart development. here we show that human lbh maps to chromosome ... | 2005 | 15958514 |
| identification of expressed genes in carp (cyprinus carpio) head kidney cells after in vitro treatment with immunostimulants. | we analysed a total of 530 expressed genes from the head kidney cells of common carp (cyprinus carpio) treated by immunostimulants. sequences of the cdna clones were compared with sequences in the genbank database. immune-related genes identified after in vitro stimulation of carp head kidney cells were: bpi/lbp, c-type lectins, fucolectins, cc-chemokine, cxc-chemokine, cd18, cyclophilin, fcgammar, g-csfr, hsp 70, ig heavy and light chains, nitr, integrin beta2-alpha, mx, interleukin-1beta, beta ... | 2005 | 15962469 |
| immunisation of fish by bath immersion using ultrasound. | ultrasonic irradiations (usi) as a means to open routes in the skin, thus facilitating the transdermal delivery of vaccines that will improve the effectiveness of vaccination by immersion, are reviewed in this paper. based on our recent results in goldfish and carp it could be summarized that: (i) usi significantly improved the antigen uptake and enhanced antibody response; (ii) the requirements for high antigen concentrations, which are needed for simple bath immersion, could be considerably re ... | 2005 | 15962476 |
| development of a cost-effective oral vaccination method against viral disease in fish. | different vaccination methods have been applied to protect fish against the detrimental effects of various pathogens. several studies have shown the potentials of oral vaccination. in theory oral vaccination is an effortless and stress-free method which can be applied at almost any age. in general, however, the vaccine has to be protected to avoid digestion, which results in high costs for application in aquaculture. in this paper we introduce a cost-effective oral vaccination strategy for viral ... | 2005 | 15962477 |
| distribution characteristics of polychlorinated biphenyls in crucian carp (carassius auratus) from major rivers in korea. | polychlorinated biphenyls (pcbs) levels in crucian carp were determined at 20 locations along four major river systems, several small-scale rivers and a wetland in korea. twenty-eight congeners, ranging from tri- to hepta-cbs were detected. a gas chromatograph with a mass selective detector was used to quantify the individual pcb congeners. the objectives of this study were to investigate the levels of contamination of pcbs in freshwater fish and to observe the pattern of their distribution. the ... | 2006 | 15964051 |
| rag expression identifies b and t cell lymphopoietic tissues during the development of common carp (cyprinus carpio). | the generation of lymphoid cells during carp development was studied by analyzing expression of the recombination activating genes (rag) using in situ hybridization and real time quantitative pcr. these data were combined with immunohistochemistry using the mab's wcl9 (cortical thymocytes) and wci12 (b cells). carp rag-1 and rag-2 showed 90 and 89% amino acid identity, respectively, to the corresponding zebrafish sequences. rag-1 was first expressed in the thymus at 4 days post-fertilization (dp ... | 2005 | 15967501 |
| characterization of glial fibrillary acidic protein and astroglial architecture in the brain of a continuously growing fish, the rainbow trout. | unlike mammals, some fish, including carp and trout, have a continuously growing brain. the glial architecture of teleost brain has been intensively studied in the carp and few data exist on trout brain. in this study, using immunoblotting we characterized the topographic distribution of glial fibrillary acidic protein (gfap) in larval and adult rainbow trout brain and studied by immunohistochemistry the distribution and morphology of gfap-immunoreactive cell systems in the rainbow trout hindbra ... | 2008 | 15967744 |
| using the nucleolar biomarker and the micronucleus test on in vivo fish fin cells. | this study was aimed at developing the nucleolar biomarker and the micronucleus test on in vivo fish fin cells for assessing water cytotoxicity and genotoxicity. both biomarkers can be used either jointly or separately on fins of the same fish during the experiment. for studying the nucleolar characteristics, small pieces of the fin edge were cut several times during 30-180 min of fish exposure. for micronucleus testing, the fin tissue regenerating after its cutting was investigated after 2-5 da ... | 2005 | 15978290 |
| concentrations of cadmium, lead, and zinc in fish from mining-influenced waters of northeastern oklahoma: sampling of blood, carcass, and liver for aquatic biomonitoring. | the tri-states mining district (tsmd) of missouri (mo), kansas (ks), and oklahoma (ok), usa, was mined for lead (pb) and zinc (zn) for more than a century. mining ceased more than 30 years ago, but wastes remain widely distributed in the region, and there is evidence of surface- and groundwater contamination in the spring river-neosho river (sr-nr) system of northeastern ok. in october 2001, we collected a total of 74 fish from six locations in the sr-nr system that included common carp (cyprinu ... | 2005 | 15981034 |
| fish rhabdoviruses: molecular epidemiology and evolution. | rhabdoviruses may cause serious diseases in wild and farmed fish. within the rhabdoviridae six genera have been established: ephemerovirus, cytorhabdovirus, nucleorhabdovirus, lyssavirus, vesiculovirus, and novirhabdovirus. viruses that infect fish are official or tentative members of the genera vesiculovirus and novirhabdovirus, or are listed as unassigned rhabdoviruses. in this report, we summarize and discuss published and our own unpublished data on the molecular epidemiology and phylogeogra ... | 2005 | 15981469 |
| characterization of transgene integration pattern in f4 hgh-transgenic common carp (cyprinus carpio l.). | the integration pattern and adjacent host sequences of the inserted pmthgh-transgene in the f4 hgh-transgenic common carp were extensively studied. here we show that each f4 transgenic fish contained about 200 copies of the pmthgh-transgene and the transgenes were integrated into the host genome generally with concatemers in a head-to-tail arrangement at 4-5 insertion sites. by using a method of plasmid rescue, four hundred copies of transgenes from two individuals of f4 transgenic fish, a and b ... | 2005 | 15987603 |
| hypocorticism and interrenal hyperplasia are not directly related to masculinization in xx mas(-1)/mas(-1) carp, cyprinus carpio. | this study reports on a homozygous xx male strain of common carp (e5), which fail to mount a normal cortisol stress response. earlier classical genetic analysis had indicated that masculinization of e5 fish was caused by a putative recessive mutation (mas(-1)/mas(-1)). hypocorticism in e5 fish was studied to investigate if it was related to masculinization. head-kidney tissues isolated from e5 fish showed a low cortisol-producing capacity in vitro, and also demonstrated a reduced sensitivity to ... | 2005 | 15993106 |
| molecular identification and expression analysis of natural resistance associated macrophage protein (nramp) cdna from japanese flounder (paralichthys olivaceus). | natural resistance associated macrophage protein (nramp) controls partially innate resistance to intracellular parasites. its function is to enhance the ability of macrophages to kill pathogens. however, little is known about the structure and function of nramp in lower vertebrates such as teleosts. we have recently isolated a cdna encoding nramp from japanese flounder (paralichthys olivaceus). the full-length cdna of the nramp is 3066 bp in length, including 224 bp 5' terminal utr, 1662 bp enco ... | 2006 | 15998592 |
| role of microfilaments and microtubules in the invasion of epc cells by aeromonas hydrophila. | the influence of the cytoskeleton on the invasion of aeromonas hydrophila strain ahj-1, isolated from diseased fish, in the monolayer cell of epithelioma papillosum cells of carp (epc) was evaluated by the recovery of gentamicin-resistant bacteria from triton x-100 cell lysates. the depolymerization of microfilaments (mf) by cytochalasin b and d inhibited the uptake of a. hydrophila in a dose-dependent manner and that of microtubules (mt) by colchicines and nocodazole did not affect the invasion ... | 2005 | 16000113 |
| multiple and highly divergent il-11 genes in teleost fish. | interleukin-11 (il-11) is a key cytokine in the regulation of proliferation and differentiation of hematopoietic progenitors and is also involved in bone formation, adipogenesis, and protection of mucosal epithelia. despite this prominent role in diverse physiological processes, il-11 has been described in only four mammalian species, and recently, in rainbow trout (oncorhynchus mykiss). here we report the presence of il-11 in common carp (cyprinus carpio), a bony fish species related to zebrafi ... | 2005 | 16003467 |
| [deterministic analysis as a tool to investigate the contingency of various components of biocenosis]. | specific application of deterministic analysis to investigate the contingencies of various components of natural biocenosis was illustrated by the example of fish production and biomass of phyto- and zooplankton. deterministic analysis confirms the theoretic assumptions on food preferences of herbivorous fish: both silver and bighead carps avoided feeding on cyanobacteria. being a facultative phytoplankton feeder, silver carp preferred microalgae to zooplankton. deterministic analysis allowed us ... | 2005 | 16004266 |