Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| development of strain-specific pcr primers for the identification of fusobacterium nucleatum subsp. fusiforme atcc 51190(t) and subsp. vincentii atcc 49256(t). | the objective of this study was to develop the strain-specific pcr primers for fusobacterium nucleatum subsp. fusiforme atcc 51190(t) and f. nucleatum subsp. vincentii atcc 49256(t) based on the nucleotide sequence of the fs17 and fv35 dna probes, respectively. the strain specificity was tested against 10 type strains of fusobacterium spp. or subsp., 21 clinical isolates of f. nucleatum from koreans, and five type strains of distinct fusobacterium species. primer sensitivity was determined by te ... | 2010 | 19398030 |
| complementation of the fada mutation in fusobacterium nucleatum demonstrates that the surface-exposed adhesin promotes cellular invasion and placental colonization. | fusobacterium nucleatum is a gram-negative oral anaerobe implicated in periodontal disease and adverse pregnancy outcome. the organism colonizes the mouse placenta, causing localized infection and inflammation. the mechanism of placental colonization has not been elucidated. previous studies identified a novel adhesin from f. nucleatum, fada, as being involved in the attachment and invasion of host cells. the fada deletion mutant f. nucleatum 12230 us1 was defective in host cell attachment and i ... | 2009 | 19398541 |
| the impact of the stone age diet on gingival conditions in the absence of oral hygiene. | the objective of this study was to assess the oral microbiota and clinical data in subjects without access to traditional oral hygiene methods and who ate a diet available in the stone age. | 2009 | 19405829 |
| decreased interleukin-2 responses to fusobacterium nucleatum and porphyromonas gingivalis in generalized aggressive periodontitis. | compromised t-cell responses to periodontal pathogens may contribute to the pathogenesis of generalized aggressive periodontitis (gagp). in this study, we attempted to characterize t-helper cell (th1, th2, and th17) responses in patients with gagp and healthy controls upon stimulation with disease-relevant pathogens. | 2009 | 19405834 |
| tgf-beta1 inhibits tlr-mediated odontoblast responses to oral bacteria. | tgf-beta1 exerts diverse functions in tooth development and tissue repair, but its role in microbial defenses of the tooth is not well-understood. odontoblasts extending their cellular processes into the dentin are the first cells to recognize signals from tgf-beta1 and bacteria in carious dentin. this study aimed to determine the role of tgf-beta1 in modulating odontoblast responses to oral bacteria. we show that these responses depend upon the expression levels of microbial recognition recepto ... | 2009 | 19407153 |
| multi-species biofilm formation on dental materials and an adhesive patch. | this laboratory study assessed the influence of surface roughness and contact time on the formation of a multi-species biofilm on dental materials (adhesive patch, composite, amalgam and enamel). | 2009 | 19408815 |
| human cytomegalovirus and epstein-barr virus inhibit oral bacteria-induced macrophage activation and phagocytosis. | periodontal disease is an inflammatory condition caused by periodontal microorganisms. viruses such as human cytomegalovirus (hcmv) and epstein-barr virus (ebv) are associated with certain types of periodontal disease, but their roles in promoting the disease are unclear. because both viruses infect human macrophages, cells which play key roles in the clearance of pathogenic bacteria, it is likely that the viruses alter the functional capacity of macrophages by inhibiting their defense mechanism ... | 2009 | 19416455 |
| polymicrobial infections, biofilms, and beyond. | 2009 | 19419439 | |
| mouse model of experimental periodontitis induced by porphyromonas gingivalis/fusobacterium nucleatum infection: bone loss and host response. | to compare the effect of oral infection with porphyromonas gingivalis or fusobacterium nucleatum versus infection with both bacteria on mouse periodontal tissues, and to characterize the inflammatory response. | 2009 | 19419440 |
| purification and partial characterization of a bacteriocin produced by an oral fusobacterium nucleatum isolate. | to purify and partially characterize a bacteriocin produced by a fusobacterium nucleatum strain. | 2009 | 19426276 |
| bacteria in the apical root canal of teeth with primary apical periodontitis. | bacteria settled in the apical root canal are in a privileged position to inflict damage to the periradicular tissues. therefore, the species identified in this region can be of special relevance for the pathogenesis of apical periodontitis. this study investigated the occurrence and levels of several bacterial taxa in the apical root canal of teeth with apical periodontitis. | 2009 | 19426923 |
| proteomics of porphyromonas gingivalis within a model oral microbial community. | porphyromonas gingivalis is a periodontal pathogen that resides in a complex multispecies microbial biofilm community known as dental plaque. confocal laser scanning microscopy showed that p. gingivalis can assemble into communities in vitro with streptococcus gordonii and fusobacterium nucleatum, common constituents of dental plaque. whole cell quantitative proteomics, along with mutant construction and analysis, were conducted to investigate how p. gingivalis adapts to this three species commu ... | 2009 | 19454014 |
| methods for optimizing dna extraction before quantifying oral bacterial numbers by real-time pcr. | methods for the optimal extraction of genomic dna for real-time pcr enumeration of oral bacteria using the muramidase, mutanolysin, were developed using a simple in vitro oral flora model comprised of the facultative anaerobic gram-positive bacteria, lactobacillus acidophilus and streptococcus mutans, the gram-positive anaerobe, parvimonas micra, and the gram-negative anaerobes, porphyromonas gingivalis, prevotella melaninogenica and fusobacterium nucleatum. traditional, as well as more elaborat ... | 2009 | 19459962 |
| fusobacterium nucleatum, the first gram-negative bacterium demonstrated to produce polyglutamate. | poly-gamma-glutamate has been described in many gram-positive organisms. when anchored to the surface, it is a capsule and as such a virulence factor. based on sequence similarities, few gram-negative organisms have been suggested to synthesize poly-gamma-glutamate. for the first time, a gram-negative bacterium, fusobacterium nucleatum, is shown to produce and secrete poly-gamma-glutamate. putative poly-gamma-glutamate-synthesizing genes from gram-negative organisms have been compared with their ... | 2009 | 19483793 |
| fetal exposure to oral pathogens and subsequent risk for neonatal intensive care admission. | maternal periodontal infection has been associated with adverse maternal and neonatal outcomes. in utero fetal exposure to oral pathogens was also recognized as deleterious to the fetus. the objective of this study was to determine the relationship between fetal exposure to oral pathogens and neonatal intensive care unit (nicu) admission. | 2009 | 19485816 |
| systemic immune responses in pregnancy and periodontitis: relationship to pregnancy outcomes in the obstetrics and periodontal therapy (opt) study. | our previous studies reported on the obstetric, periodontal, and microbiologic outcomes of women participating in the obstetrics and periodontal therapy (opt) study. this article describes the systemic antibody responses to selected periodontal bacteria in the same patients. | 2009 | 19485826 |
| [detection of luxs gene of quorum sensing signal system in periodontal pathogen]. | to detect the presence and distribution of luxs gene in quorum sensing signal system in the periodontal pathogens. | 2009 | 19489256 |
| bacterial colonization of enamel in situ investigated using fluorescence in situ hybridization. | oral biofilms are one of the greatest challenges in dental research. the present study aimed to investigate initial bacterial colonization of enamel surfaces in situ using fluorescence in situ hybridization (fish) over a 12 h period. for this purpose, bovine enamel slabs were fixed on buccal sites of individual splints worn by six subjects for 2, 6 and 12 h to allow biofilm formation. specimens were processed for fish and evaluated with confocal laser-scanning microscopy, using probes for eubact ... | 2009 | 19528150 |
| starvation response and growth in serum of fusobacterium nucleatum, peptostreptococcus anaerobius, prevotella intermedia, and pseudoramibacter alactolyticus. | the microbiota inhabiting the untreated root canal differ markedly from those found in post-treatment disease, yet there is limited information on the microbial characteristics distinguishing the different infections. we hypothesized that starvation survival is a key microbial property in species selection. this study analyzed starvation-survival behavior over 60 days of species representative of the untreated root canal infection: fusobacterium nucleatum, peptostreptococcus anaerobius, prevotel ... | 2009 | 19540450 |
| a 21-year-old man with fever and abdominal pain after recent peritonsillar abscess drainage. | lemierre syndrome an extremely rare and unreported disease typically results from oropharyngeal infection and/or local tissue trauma with invasion of the parapharyngeal soft tissue with bacteria. once local tissue invasion occurs, internal jugular vein septic thrombophlebitis with or without metastatic complications may occur. the etiology of lemierre syndrome is generally fusobacterium necrophorum, reported to be present in approximately 80% of the cases. the outcome of lemierre syndrome in the ... | 2009 | 19555636 |
| real-time polymerase chain reaction to determine the prevalence and copy number of epstein-barr virus and cytomegalovirus dna in subgingival plaque at individual healthy and periodontal disease sites. | detection of cytomegalovirus (cmv) and epstein-barr virus (ebv) in plaque from patients with periodontal disease provides support for the theory that these viruses play a role in the pathogenesis of periodontitis. this study sought to further define this relationship by determining the prevalence of these viruses at individual disease and healthy sites of patients with periodontal disease and to determine whether the presence and amount of viral dna correlate with disease severity. | 2009 | 19563294 |
| fusobacterium nucleatum enters normal human oral fibroblasts in vitro. | fusobacterium nucleatum, a commensal opportunistic oral bacterium, is capable of invading gingival epithelial cells, but the entrance into human primary oral fibroblast cells has not been documented. this study evaluated the ability of three strains of f. nucleatum (f. nucleatum ssp. nucleatum, f. nucleatum ssp. polymorphum, and f. nucleatum ssp. vincentii) to enter gingival fibroblasts (gfs) and periodontal ligament fibroblasts (plfs). | 2009 | 19563299 |
| comparison between polymerase chain reaction-based and checkerboard dna hybridization techniques for microbial assessment of subgingival plaque samples. | to compare polymerase chain reaction (pcr) with subsequent reverse hybridization (micro-ident test) and checkerboard dna-dna hybridization for the identification of 13 bacterial species in subgingival plaque samples. | 2009 | 19563330 |
| aggregatibacter actinomycetemcomitans builds mutualistic biofilm communities with fusobacterium nucleatum and veillonella species in saliva. | human oral bacterial pathogens grow in attached multispecies biofilm communities. unattached cells are quickly removed by swallowing. therefore, surface attachment is essential for growth, and we investigated multispecies community interactions resulting in mutualistic growth on saliva as the sole nutritional source. we used two model systems, saliva-coated transferable solid-phase polystyrene pegs (peg biofilms) and flow cells with saliva-coated glass surfaces. fluorescent antibody staining and ... | 2009 | 19564387 |
| proteomic analysis of endodontic infections by liquid chromatography-tandem mass spectrometry. | endodontic infections are very prevalent and have a polymicrobial etiology characterized by complex interrelationships between endodontic microorganisms and the host defenses. proteomic analysis of endodontic infections can provide global insights into the invasion, pathogenicity mechanisms, and multifactorial interactions existing between root canal bacteria and the host in the initiation and progression of apical periodontitis. the purpose of this study was to apply proteomic techniques such a ... | 2009 | 19572900 |
| change in diet and oral hygiene over an 8-week period: effects on oral health and oral biofilm. | the aim of the study was to monitor changes in oral health and oral biofilm composition in vivo during an experiment simulating prehistoric lifestyle and diet and poor oral hygiene. thirteen subjects lived for a period of 8 weeks under neolithic conditions. the following clinical parameters were recorded before and after the project: gingival and plaque index (löe and silness, acta odontol scand 21:533, 1963; silness and löe, acta odontol scand 22:121-135, 1964), probing pocket depth, and bleedi ... | 2010 | 19626350 |
| mixed infection involving actinomyces, aggregatibacter, and fusobacterium species presenting as perispinal tumor. | a representative case in which a polymicrobial infection involving fusobacterium nucleatum, actinomyces israelii and aggregatibacter (formerly actinobacillus) actinomycetemcomitans was initially diagnosed as malignancy in an edentulous patient. additional history obtained after the nature of the syndrome was elucidated revealed that he had had his two remaining teeth extracted four months prior to this episode. | 2010 | 19628046 |
| [preliminary study on the discrimination of putative periodontal pathogens with a metabonomics method]. | to evaluate the feasibility of identifying oral pathogenic bacteria by comparing the metabolic profiling of putative periodontal pathogens and try to find a convenient and rapid way to discriminate oral microorganisms. | 2009 | 19637485 |
| bone microbial contamination influences autogenous grafting in sinus augmentation. | the oral occurrence of putative microbial pathogens in humans has been documented in health and disease. the presence of periodontopathogens in patients with a history of periodontal disease may have a negative impact on bone regeneration. this investigation was conducted to confirm the presence of periodontal pathogens in bone particles harvested intraorally for maxillary sinus augmentation and to assess the clinical and radiographic outcomes 6 to 12 months after bone augmentation. | 2009 | 19656037 |
| quantitative detection of periodontopathic bacteria in atherosclerotic plaques from coronary arteries. | oral pathogens, including periodontopathic bacteria, are thought to be aetiological factors in the development of cardiovascular disease. in this study, the presence of aggregatibacter actinomycetemcomitans, fusobacterium nucleatum-periodonticum-simiae group, porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens and tannerella forsythia in atheromatous plaques from coronary arteries was determined by real-time pcr. forty-four patients displaying cardiovascular disease were submi ... | 2009 | 19679682 |
| use of 2-d electrophoresis and esi mass spectrometry techniques to characterize fusobacterium nucleatum proteins up-regulated after oxidative stress. | the genus fusobacterium belongs to the fusobacteriaceae family and is a gram-negative obligate anaerobic bacterium found in the human oral microbiota. even that fusobacterium nucleatum cannot grow under aerobic conditions, they may exhibit aerotolerance as an adaptive response which could figure as an important virulence factor, during the stages of infection, when these anaerobes are shifted to aerobic conditions. in this regard, little is known about bacterial oxidative stress adaptive respons ... | 2010 | 19683589 |
| quantification of periodontal pathogens by paper point sampling from the coronal and apical aspect of periodontal lesions by real-time pcr. | the present study compared the recovery of six periodontal pathogens by paper point samples from two different aspects of periodontal lesions by quantitative real-time polymerase chain reaction (pcr). twenty patients with untreated chronic periodontitis were randomized into two groups. before subgingival instrumentation and after 10 weeks samples in group a were taken first with a paper point half length (hp) of the probing depth, then with a paper point full length (fp) at the same site. in gro ... | 2010 | 19693550 |
| assessment of intraradicular bacterial composition by terminal restriction fragment length polymorphism analysis. | the aim of the study was to assess the bacterial community structures associated with endodontic infections using terminal restriction fragment length polymorphism (t-rflp), and to investigate the correlation of whole community profiles with the manifestation of particular clinical features. | 2009 | 19702949 |
| oral bacteria induce a differential activation of human immunodeficiency virus-1 promoter in t cells, macrophages and dendritic cells. | the human immunodeficiency virus (hiv) can integrate into t cells, macrophages and dendritic cells resulting in a latent infection. reports have also demonstrated that various microbial and host cell factors can trigger hiv reactivation leading to hiv recrudescence, potentially undermining highly active antiretroviral therapies. | 2009 | 19702954 |
| the effect of lactoferrin on oral bacterial attachment. | lactoferrin (lf), an iron-binding salivary glycoprotein, plays an important role in human innate defense against local mucosal infection. we hypothesized that lf interferes with initial oral bacterial attachment to surfaces by iron sequestration, so inhibiting subsequent biofilm formation. the objective was to investigate the effect of lf on the early stages of single-species and multi-species oral biofilm development. | 2009 | 19702956 |
| contemporary root canal irrigants are able to disrupt and eradicate single- and dual-species biofilms. | clinical/microbiological studies have consistently revealed the persistence of some bacteria after conventional root canal debridement. although this was originally attributed to the complexity of the root canal anatomy and the difficulty of delivering antibacterial agents effectively, it has emerged that the biofilm encasement of bacterial cells may confer a further mechanism of resistance. the purpose of this study was to investigate the relative disruption and bactericidal effects of root can ... | 2009 | 19720223 |
| maternal periodontal disease and soluble fms-like tyrosine kinase-1 expression. | this study was conducted to examine the relationship between maternal periodontal disease and plasma angiogenic factor expression of soluble fms-like tyrosine kinase (sflt)-1. | 2009 | 19722802 |
| fusobacterium nucleatum endocarditis mimicking polymyalgia rheumatica. | a case of fusobacterium nucleatum endocarditis in an 80-year-old man is reported. the patient presented with a headache and nonspecific musculoskeletal symptoms and was misdiagnosed as having polymyalgia rheumatica. the diagnosis of bacterial endocarditis was delayed because of an insidious presentation, typical in infections with low virulence micro-organisms. the musculoskeletal symptoms, unresponsive to protracted corticosteroids, completely resolved with intravenous ampicillin treatment. rhe ... | 2009 | 19738533 |
| mutualistic biofilm communities develop with porphyromonas gingivalis and initial, early, and late colonizers of enamel. | porphyromonas gingivalis is present in dental plaque as early as 4 h after tooth cleaning, but it is also associated with periodontal disease, a late-developing event in the microbial successions that characterize daily plaque development. we report here that p. gingivalis atcc 33277 is remarkable in its ability to interact with a variety of initial, early, middle, and late colonizers growing solely on saliva. integration of p. gingivalis into multispecies communities was investigated by using t ... | 2009 | 19749049 |
| mobilization and prevalence of a fusobacterial plasmid. | fusobacterium nucleatum is a gram-negative anaerobic rod found in dental plaque biofilms, and is an opportunistic pathogen implicated in periodontitis as well as a wide range of systemic abscesses and infections. genomic analyses of f. nucleatum indicate considerable genetic diversity and a prominent role for horizontal gene transfer in the evolution of the species. several plasmids isolated from f. nucleatum, including pfn1, harbor relaxase gene homologs that may function in plasmid mobilizatio ... | 2010 | 19761790 |
| an unusual autopsy case of pyogenic liver abscess caused by periodontal bacteria. | pyogenic liver abscess (pla) formation is thought to originate from the transmission of infection via three major routes including the biliary tract, portal vein and hepatic artery. however, about 50% of pla cases are considered to be cryptogenic. here we report an unusual autopsy case of pla associated with periodontopathic bacterial infection. a 59-year-old female suddenly developed cardiopulmonary arrest and died. despite macroscopic and microscopic examinations, the infectious routes and sou ... | 2009 | 19762989 |
| kinetic characterization and quaternary structure of glutamate racemase from the periodontal anaerobe fusobacterium nucleatum. | cofactor-independent glutamate racemases (grs) that supply the d-glutamate required for biosynthesis of the peptidoglycan that encapsulates bacterial cells are attractive targets for the development of antibacterial drugs. recombinant gr from fusobacterium nucleatum (fngr), a gram-negative anaerobe involved in periodontal disease, was overproduced, purified, and characterized. unlike most other grs, fngr is a pseudosymmetric enzyme, catalyzing the racemization of glutamate enantiomers with simil ... | 2009 | 19772853 |
| differential ability of periodontopathic bacteria to modulate invasion of human gingival epithelial cells by porphyromonas gingivalis. | periodontitis is a polymicrobial infection caused by selected gram-negative bacteria including porphyromonas gingivalis. host cell invasion by p. gingivalis has been proposed as a possible mechanism of pathogenesis in periodontitis. the aim of the present study was to assess the influence of periodontopathogens on p. gingivalis invasion of gingival epithelial cells in polymicrobial infection. p. gingivalis was tested for its ability to invade a human gingival epithelial cell line ca9-22 in co-in ... | 2009 | 19818393 |
| photodynamic therapy of persistent pockets in maintenance patients-a clinical study. | the aim of this study was to compare the short-term performance of a session of single photodynamic therapy (pdt) and of a conventional ultrasonic debridement (ust) in persistent pockets of maintenance patients. in a prospective, randomized, controlled, single-blind clinical study, patients with chronic periodontitis with at least two persistent pockets (>4 mm) were enrolled. they were treated either with ust (n = 29) or pdt (n = 25). clinical and microbiological examinations were performed at b ... | 2010 | 19823880 |
| microbial interactions influence inflammatory host cell responses. | the inflammatory response plays an important role in the tissue destruction associated with periodontitis. bacterial species can regulate the inflammatory responses of host cells, triggered by pathogens. it was hypothesized that, in the field of oral microbiology/immunology, such effects of bacterial interactions on inflammatory host cell responses might also be present. in this study, the effects of beneficial, commensal, and pathogenic species on aggregatibacter actinomycetemcomitans-induced i ... | 2009 | 19828891 |
| involvement of cytosolic phospholipase a2alpha in mmp-9 up-regulation. | matrix metalloproteinase-9 (mmp-9) is important in the pathogenesis of periodontitis. cytosolic phospholipase a(2)alpha (cpla(2)alpha) is involved in mmp-9 up-regulation in human monocytes. we tested the hypothesis that cpla(2)alpha also regulates mmp-9 induction by fusobacterium nucleatum and by phorbol 12-myristate-13-acetate (pma) in gingival epithelial cells. while pma induced mmp-9 expression considerably, f. nucleatum did so moderately. this time-course study demonstrated that mmp-9 mrna u ... | 2009 | 19828892 |
| progression of chronic periodontitis can be predicted by the levels of porphyromonas gingivalis and treponema denticola in subgingival plaque. | chronic periodontitis is an inflammatory disease of the supporting tissues of the teeth associated with bacteria. diagnosis is achieved retrospectively by clinical observation of attachment loss. predicting disease progression would allow for targeted preventive therapy. the aim of this study was to monitor disease progression in patients on a maintenance program and determine the levels of specific bacteria in subgingival plaque samples and then examine the ability of the clinical parameters of ... | 2009 | 19832799 |
| characterization of bacterial flora in persistent apical periodontitis lesions. | microorganisms are able to survive and induce persistent infection in periapical tissues. the aim of this study was to investigate the composition of the microflora of persistent apical periodontitis lesions. | 2009 | 19832803 |
| autoaggregation response of fusobacterium nucleatum. | fusobacterium nucleatum is a gram-negative oral bacterial species associated with periodontal disease progression. this species is perhaps best known for its ability to adhere to a vast array of other bacteria and eukaryotic cells. numerous studies of f. nucleatum have examined various coaggregation partners and inhibitors, but it is largely unknown whether these interactions induce a particular genetic response. we tested coaggregation between f. nucleatum atcc strain 25586 and various species ... | 2009 | 19837836 |
| antibiotic resistance of periodontal pathogens obtained from frequent antibiotic users. | the occurrence of bacterial strains resistant to different antimicrobials is a growing problem in latin american countries. the aim was to measure the antimicrobial susceptibility of fusobacterium nucleatum, prevotella intermedia and prophyromona gingivalis, isolated from chronic periodontitis patients. twenty-five patients diagnosed with generalized- moderate or advanced- chronic periodontitis were consecutively recruited from patients attending the periodontal specialist program, javeriana uni ... | 2009 | 19839485 |
| hydrogen sulfide production from cysteine and homocysteine by periodontal and oral bacteria. | hydrogen sulfide is one of the predominant volatile sulfur compounds (vscs) produced by oral bacteria. this study developed and evaluated a system for detecting hydrogen sulfide production by oral bacteria. | 2009 | 19905954 |
| sonic extracts from a bacterium related to periapical disease activate gelatinase a and inactivate tissue inhibitor of metalloproteinases timp-1 and timp-2. | to examine the effects of sonicated bacterial extracts (sbes) from three related to periapical disease bacteria (porphyromonas gingivalis, p. endodontalis and f. nucleatum) on the activation of matrix metalloproteinase (mmp-2) and the inactivation of tissue inhibitors of metalloproteinase (timp-1 and timp-2). | 2009 | 19912382 |
| acute appendicitis is characterised by local invasion with fusobacterium nucleatum/necrophorum. | acute appendicitis is a local intestinal inflammation with unclear origin. the aim was to test whether bacteria in appendicitis differ in composition to bacteria found in caecal biopsies from healthy and disease controls. | 2011 | 19926616 |
| methamphetamine cytotoxicity and effect on lps-stimulated il-1beta production by human monocytes. | methamphetamine (meth) abuse is associated with "meth mouth", characterized by rampant dental decay and destruction of periodontal bone and soft tissues. in periodontitis, monocyte/macrophages, stimulated by bacterial lipopolysaccharide (lps), produce interleukin-1beta (il-1beta), contributing to bone and soft tissue degradation. effects of meth on monocyte/macrophages and its role in periodontitis are unknown. the objective of this study was to determine meth cytotoxicity and effects on constit ... | 2010 | 19945523 |
| fusobacterium bacteremia: clinical significance and outcomes. | fusobacterium bacteremia is uncommon, accounting for approximately 0.9% of patients with bacteremia. the objectives of this study were to evaluate the incidence and clinical significance of blood cultures positive for fusobacterium spp., risk factors for mortality, and the impact of antimicrobial therapy on clinical outcomes. | 2009 | 19949758 |
| application of rpob and zinc protease gene for use in molecular discrimination of fusobacterium nucleatum subspecies. | fusobacterium nucleatum is classified into five subspecies that inhabit the human oral cavity (f. nucleatum subsp. nucleatum, f. nucleatum subsp. polymorphum, f. nucleatum subsp. fusiforme, f. nucleatum subsp. vincentii, and f. nucleatum subsp. animalis) based on several phenotypic characteristics and dna-dna hybridization patterns. however, the methods for detecting or discriminating the clinical isolates of f. nucleatum at the subspecies levels are laborious, expensive, and time-consuming. the ... | 2010 | 19955278 |
| transmission of periodontopathic bacteria from natural teeth to implants. | purpose: prevention of peri-implantitis is essential for the success of implant rehabilitation. infection by periodontopathic bacteria is a major cause of peri-implantitis. the aim of the present study was to identify the source of peri-implant colonization by periodontopathic bacteria. materials and methods: twenty-one patients with implants were enrolled in the study. subgingival plaque samples from the adjacent, occluding, and contralateral natural teeth were collected prior to second-stage s ... | 2009 | 20002682 |
| identification of bacteria enduring endodontic treatment procedures by a combined reverse transcriptase-polymerase chain reaction and reverse-capture checkerboard approach. | this study identified the bacterial taxa enduring endodontic treatment procedures by using a combined 16s ribosomal rna-based reverse-transcriptase polymerase chain reaction (rt-pcr) and reverse-capture checkerboard hybridization approach. | 2010 | 20003934 |
| fusobacterium nucleatum envelope protein foma is immunogenic and binds to the salivary statherin-derived peptide. | we have previously shown that one of the minimal active regions of statherin, a human salivary protein, for binding to fusobacterium nucleatum is a yqpvpe amino acid sequence. in this study, we identified the foma protein of f. nucleatum, which is responsible for binding to the statherin-derived yqpvpe peptide. overlay analysis showed that a 40-kda protein of the f. nucleatum cell envelope (40-kda ce) specifically bound to the yqpvpe peptide. the equilibrium association constant between the affi ... | 2010 | 20008529 |
| bacterial adhesins to host components in periodontitis. | 2010 | 20017793 | |
| nod1 and nod2 mediate sensing of periodontal pathogens. | in bacterial infection, nucleotide-binding oligomerization domain (nod) 1 and nod2 induce innate immune responses by recognizing fragments of the bacterial component peptidoglycan (pgn). to determine the roles of these receptors in detection of periodontal pathogens, we stimulated human embryonic kidney cells expressing nod1 or nod2 with heat-killed porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, and fusobacterium nucleatum or their soluble pgns (spgns). all bacteria and their s ... | 2010 | 20040739 |
| microbiologic testing and outcomes of full-mouth scaling and root planing with or without amoxicillin/metronidazole in chronic periodontitis. | it has been suggested that use of systemic antibiotics should be limited to patients with specific microbiologic profiles. the main purpose of the present analysis was to study whether microbiologic testing before therapy was of value in predicting which patients would specifically benefit from adjunctive amoxicillin and metronidazole, given in the context of full-mouth scaling and root planing (srp) within 48 hours. | 2010 | 20059413 |
| in vitro killing activity of crevicular concentrations of tinidazole plus common oral antibiotics against high-density mixed inocula of periodontal pathogens in strict anaerobic conditions. | odontogenic infections are polymicrobial. this study explores the in vitro killing activity by concentrations similar to those found in crevicular fluid of tinidazole in combination with amoxicillin/clavulanic acid, clindamycin and levofloxacin against four groups of high-density mixed inocula of anaerobes (prevotella buccae, fusobacterium nucleatum, and veillonella spp.) and facultative (capnocytophaga spp. and streptococcus spp.) isolates of periodontal pathogens. | 2010 | 20059425 |
| [pelvic actinomycosis: a misleading picture]. | pelvic actinomycosis is a rare chronic suppurative granulomatous disease associated with an intrauterine contraceptive device. next pelvic organs can be affected but also more distant organs, to lead to different clinical situations. the diagnosis is therefore often difficult. we report here the case of a woman who presents a particular extensive pelvic actinomycosis diagnosed after surgical treatment with important morbidity. this case shows the interest of evoking the diagnosis in an evocative ... | 2010 | 20074892 |
| substrate-dependent proton antiport in neurotransmitter:sodium symporters. | neurotransmitter-sodium symporters (nss), targets for psychostimulants and therapeutic drugs, have a critical role in neurotransmission. whereas eukaryotic nss show chloride-dependent transport, bacterial nss feature cl(-)-independent substrate transport. recently we showed that mutation of an acidic residue near one of the sodium ion-binding sites in leut of aquifex aeolicus or tyt1 of fusobacterium nucleatum renders substrate binding and/or transport cl(-) dependent. we reasoned that the negat ... | 2010 | 20081826 |
| efficacy of a low-concentration chlorhexidine mouth rinse in non-compliant periodontitis patients attending a supportive periodontal care programme: a randomized clinical trial. | to assess the clinical and microbiological efficacy of a 0.05% chlorhexidine and 0.05% cetyl-pyridinium chloride mouth rinse in supportive periodontal care (spc) in patients with inadequate plaque control. | 2010 | 20088984 |
| use of a novel assay to evaluate enzymes that produce hydrogen sulfide in fusobacterium nucleatum. | to evaluate enzymes that produce hydrogen sulfide (h(2)s) in species of fusobacterium nucleatum, we developed an assay based on sds-polyacrylamide gel electrophoresis with renaturation followed by active staining. this assay provided precise insight into the enzymes that produce h(2)s in terms of their number and molecular weights. | 2010 | 20093145 |
| term stillbirth caused by oral fusobacterium nucleatum. | intrauterine infection is a recognized cause of adverse pregnancy outcome, but the source of infection is often undetermined. we report a case of stillbirth caused by fusobacterium nucleatum that originated in the mother's mouth. | 2010 | 20093874 |
| epithelial cell pro-inflammatory cytokine response differs across dental plaque bacterial species. | the dental plaque is comprised of numerous bacterial species, which may or may not be pathogenic. human gingival epithelial cells (hgecs) respond to perturbation by various bacteria of the dental plaque by production of different levels of inflammatory cytokines, which is a putative reflection of their virulence. the aim of the current study was to determine responses in terms of interleukin (il)-1beta, il-6, il-8 and il-10 secretion induced by porphyromonas gingivalis, aggregatibacter actinomyc ... | 2010 | 20096064 |
| the bactericidal effect of a genius nd:yag laser. | to evaluate the 'in vitro' bactericidal effect of the nd:yag laser (genius, mølsgaard dental, copenhagen, denmark) on six periodontal pathogens. | 2010 | 20096084 |
| effects of methyl gallate and gallic acid on the production of inflammatory mediators interleukin-6 and interleukin-8 by oral epithelial cells stimulated with fusobacterium nucleatum. | interactions between periodontal bacteria and human oral epithelial cells can lead to the activation and expression of a variety of inflammatory mediators in epithelial cells. fusobacterium nucleatum is a filamentous human pathogen that is strongly associated with periodontal diseases. this study examined the effects of methyl gallate (mg) and gallic acid (ga) on the production of inflammatory mediators, interleukin (il)-6 and il-8, by oral epithelial cells stimulated by f. nucleatum. in a real- ... | 2010 | 20127471 |
| the antimicrobial efficacy of commercial dentifrices. | this investigation compared the effects of a fluoride dentifrice and toothpastes formulated with antimicrobial ingredients (stannous fluoride and triclosan/copolymer) on oral micro-organisms, including those found in samples taken from the human oral cavity. microbiological techniques determined the minimum inhibitory concentrations (mics) of each dentifrice necessary to inhibit the growth of bacterial strains from the healthy oral cavity, as well as those found in dental caries, periodontal dis ... | 2010 | 20129893 |
| effects of a mouthwash with chlorine dioxide on oral malodor and salivary bacteria: a randomized placebo-controlled 7-day trial. | previous research has shown the oxidizing properties and microbiological efficacies of chlorine dioxide (clo2). its clinical efficacies on oral malodor have been evaluated and reported only in short duration trials, moreover, no clinical studies have investigated its microbiological efficacies on periodontal and malodorous bacteria. thus, the aim of this study was to assess the inhibitory effects of a mouthwash containing clo2 used for 7 days on morning oral malodor and on salivary periodontal a ... | 2010 | 20152022 |
| central role of the early colonizer veillonella sp. in establishing multispecies biofilm communities with initial, middle, and late colonizers of enamel. | human dental biofilm communities comprise several species, which can interact cooperatively or competitively. bacterial interactions influence biofilm formation, metabolic changes, and physiological function of the community. lactic acid, a common metabolite of oral bacteria, was measured in the flow cell effluent of one-, two- and three-species communities growing on saliva as the sole nutritional source. we investigated single-species and multispecies colonization by using known initial, early ... | 2010 | 20154130 |
| the relationship between physiologic halitosis and periodontopathic bacteria of the tongue and gingival sulcus. | to determine the influence of oral status on halitosis, the relationship between halitosis and periodontopathic bacteria present in plaque on the tongue and the subgingival sulcus was examined in 62 periodontally healthy adults. halitosis indicators used were the organoleptic score; gas chromatography results [total volatile sulfur compounds (vscs) = h(2)s + ch(3)sh + (ch(3))(2)s]; halimeter values; and the results of three clinical tests, plaque control record (plcr), plaque index (pli), and to ... | 2010 | 20155507 |
| lemierre syndrome: a japanese patient returning from thailand. | after returning from thailand, a 23-year-old japanese man was admitted because of fever, sore throat, neck pain, and chest pain. contrasted-enhanced ct scanning of his neck revealed an absence of flow through the right internal jugular vein representing thrombosis, and moreover, an increase in wall thickness of the right internal jugular vein and enhancement of the surrounding tissue representing thrombophlebitis. lung abscesses were also identified by a chest ct scan. fusobacterium nucleatum wa ... | 2010 | 20177952 |
| vaccination targeting surface foma of fusobacterium nucleatum against bacterial co-aggregation: implication for treatment of periodontal infection and halitosis. | the mechanical therapy with multiple doses of antibiotics is one of modalities for treatment of periodontal diseases. however, treatments using multiple doses of antibiotics carry risks of generating resistant strains and misbalancing the resident body flora. we present an approach via immunization targeting an outer membrane protein foma of fusobacterium nucleatum (f. nucleatum), a central bridging organism in the architecture of oral biofilms. neutralization of foma considerably abrogated the ... | 2010 | 20189489 |
| quantification of five putative periodontal pathogens in female patients with and without chronic periodontitis by real-time polymerase chain reaction. | chronic periodontitis is a highly prevalent endogenous polymicrobial disease. to better understand the etiology of the disease a quantitative approach is mandatory and real-time pcr is the molecular technique currently preferred to achieve this purpose. taking into account that such a kind of study is still scarce, we aimed to evaluate the association between periodontal microbiota and chronic periodontitis. a total of 60 low-income age-matched female adults, 30 with chronic periodontitis and 30 ... | 2010 | 20193770 |
| oral biofilm architecture on natural teeth. | periodontitis and caries are infectious diseases of the oral cavity in which oral biofilms play a causative role. moreover, oral biofilms are widely studied as model systems for bacterial adhesion, biofilm development, and biofilm resistance to antibiotics, due to their widespread presence and accessibility. despite descriptions of initial plaque formation on the tooth surface, studies on mature plaque and plaque structure below the gum are limited to landmark studies from the 1970s, without app ... | 2010 | 20195365 |
| [anaerobic gram-negative bacilli involved in the etiopathogeny of the abscesses of superficial fascial spaces of the face and neck]. | the aim of this study was to isolate and identify at species level the strains of anaerobic gram-negative bacilli (gnb) from pus samples collected in patients with abscesses of fascial spaces of the face and neck. microscopy of gram-stained smears and cultures were performed in each specimen. the strictly anaerobic gnb strains were identified using the conventional methods of diagnosis and the rapid id 32 a system. in addition, the other strains isolated in association with these bacteria were i ... | 2008 | 20201270 |
| interplay of protease-activated receptors and nod pattern recognition receptors in epithelial innate immune responses to bacteria. | protease-activated receptors (pars), nucleotide-binding oligomerization domain (nod) receptors and toll-like receptors (tlrs) play a role in innate immunity, but little is known about interaction between these receptors. the goal of this study was to investigate how silencing one receptor affects the expression of other receptors and downstream innate immune markers in response to bacteria. human gingival epithelial cells (gecs) were transfected with sirna specific for par1 or par2, then stimula ... | 2010 | 20219537 |
| detection of periodontopathogenic bacteria in pregnant women by traditional anaerobic culture method and by a commercial molecular genetic method. | to culture facultative and strict anaerobic bacteria is a well-established method for analyzing subgingival plaque samples. micro-ident and micro-ident plus (hain lifescience gmbh, nehren, germany) tests are two commercially available rapid pcr-based methods for the identification and quantification of putative periodontopathogen bacteria. in this study, we compared these commercial pcr-based hybridization methods with conventional anaerobic culture technique. a total of 36 subgingival plaque sa ... | 2010 | 20219690 |
| potentiation of bacterial killing activity of zinc chloride by pyrrolidine dithiocarbamate. | zinc has antimicrobial activity and zinc salts including zinc chloride (zncl(2)) have been used for the control of oral malodor. in this study, we hypothesized that pyrrolidine dithiocarbamate (pdtc), a zinc ionophore, may enhance antimicrobial efficacy of zncl(2). the bactericidal effectiveness of zncl(2) alone (0.5-8 mm) or in combination with pdtc (1 or 10 microm) was evaluated by in vitro short (1 h) time-killing assays against fusobacterium nucleatum and porphyromonas gingivalis. only a sli ... | 2010 | 20221728 |
| antimicrobial effect of rosa damascena extract on selected endodontic pathogens. | the purpose of this study was to compare the antimicrobial activity of a plant-derived extract (2 percent rosa damascena extract) with 5.25 percent sodium hypochlorite (naocl) and 2 percent chlorhexidine (chx) on selected endodontic pathogens. the minimum inhibitory concentrations (mics) of 2 percent rose extract and 2 percent chx for test microorganisms, except f. nucleatum, were lower than that of 5.25 percent naocl. all solutions were able to kill all test microorganisms after one minute. | 2010 | 20232691 |
| photodynamic therapy in periodontal therapy: microbiological observations from a private practice. | in recent years, the combination of laser light and photosensitizer known as photodynamic therapy (pdt) has been used in periodontal therapy. however, there are not enough clinical studies to fully evaluate the effects of pdt on the periodontal tissues. this microbiological study examined the effects of pdt on the periodontal bacteria in combination with scaling and root planing (srp) in the same group of patients by randomly selecting pdt or srp for use in different quadrants of the mouth. for ... | 2010 | 20236906 |
| effect of alkaline growth ph on the expression of cell envelope proteins in fusobacterium nucleatum. | fusobacterium nucleatum is a gram-negative anaerobic organism that plays a central role in the development of periodontal diseases. the progression of periodontitis is associated with a rise in ph of the gingival sulcus which promotes the growth and expression of virulence factors by periodontopathic bacteria. we have previously reported that the expression of specific cytoplasmic proteins is altered by a shift in growth ph. in the present study we have compared cell envelope protein expression ... | 2010 | 20299401 |
| endodontic photoactivated disinfection using a conventional light source: an in vitro and ex vivo study. | the antimicrobial effect of photoactivated disinfection (pad) using toluidine blue and an led lamp was tested on endodontic pathogens in planktonic suspension and after inoculation into extracted teeth. irradiation time was limited to 30 seconds. | 2010 | 20303060 |
| a novel bioactivity of omega-3 polyunsaturated fatty acids and their ester derivatives. | fish oil, enriched in omega-3 polyunsaturated fatty acids (n-3 pufa), is widely used as a dietary or nutritional supplement with numerous benefits, including as an anti-inflammatory particularly linked to atherosclerosis. while n-3 pufa have been suggested to be able to improve oral health through a reduction in inflammation through elevations in these fatty acids in serum and cellular membranes, information is lacking for the possibility that these fatty acids could directly impact the survival ... | 2010 | 20331795 |
| supernatants from oral epithelial cells and gingival fibroblasts modulate human immunodeficiency virus type 1 promoter activation induced by periodontopathogens in monocytes/macrophages. | bacterial and host cell products during coinfections of human immunodeficiency virus type 1-positive (hiv-1(+)) patients regulate hiv-1 recrudescence in latently infected cells (e.g. t cells, monocytes/macrophages), impacting highly active antiretroviral therapy (haart) failure and progression of acquired immunodeficiency syndrome. a high frequency of oral opportunistic infections (e.g. periodontitis) in hiv-1(+) patients has been demonstrated; however, their potential to impact hiv-1 exacerbati ... | 2010 | 20331801 |
| full-mouth antimicrobial photodynamic therapy in fusobacterium nucleatum-infected periodontitis patients. | the purpose of this study is to clinically and microbiologically evaluate the effect of photodynamic therapy (pdt) as a full-mouth procedure in fusobacterium nucleatum-infected patients with periodontitis. | 2010 | 20350153 |
| biofilm formation enhances the oxygen tolerance and invasiveness of fusobacterium nucleatum in an oral mucosa culture model. | the present study evaluates the survival capability of fusobacterium nucleatum strains in an aerobic environment and compares the invasive capability of f. nucleatum in biofilm and planktonic forms in an organotypic cell culture (occ) model. | 2010 | 20350156 |
| case records of the massachusetts general hospital. case 10-2010. a 37-year-old woman with weakness and a mass in the brain. | 2010 | 20375410 | |
| mixture of periodontopathogenic bacteria influences interaction with kb cells. | the purpose of this study was to investigate the adhesion and invasion of periodontopathogenic bacteria in varied mixed infections and the release of interleukins from an epithelial cell line (kb cells). | 2010 | 20380884 |
| antimicrobial activity of super-oxidised water against oral microorganisms. | the radical anion of oxygen (o(-)) is extremely oxidative and shows high reactivity. in this study, the antibacterial activity of water super-oxidised water containing high concentration of o(-) (o(-)-water) was tested against cultured planktonic cells of cariogenic bacteria, periodontopathic bacteria and candida albicans. | 2010 | 20381790 |
| detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16s rrna gene pcr. | we describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16s rrna gene pcr. simultaneous detection of fusobacterium nucleatum and porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16s rrna genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis. | 2010 | 20392909 |
| [effect of periodontal mechanical treatment on periodontal pathogenic bacteria in gingival crevicular fluid of chronic periodontitis patients]. | to evaluate the subgingival prevalent rates of 6 periodontal pathogenic bacteria in gingival crevicular fluids of cp patients before and after treatment, to analyze the relationship between the prevalent variance and periodontal clinical parameters, and to provide a microbiologic method of evaluating curative effect and estimating the prognosis. | 2010 | 20396365 |
| production of hydrogen sulfide by two enzymes associated with biosynthesis of homocysteine and lanthionine in fusobacterium nucleatum subsp. nucleatum atcc 25586. | fusobacterium nucleatum produces a large amount of the toxic metabolite hydrogen sulfide in the oral cavity. here, we report the molecular basis of f. nucleatum h(2)s production, which is associated with two different enzymes: the previously reported cdl (fn1220) and the newly identified lcd (fn0625). sds-page analysis with activity staining revealed that crude enzyme extracts from f. nucleatum atcc 25586 contained three major h(2)s-producing proteins. two of the proteins with low molecular mass ... | 2010 | 20413556 |
| innate inflammatory responses of human decidual cells to periodontopathic bacteria. | the purpose of this study was to test the hypothesis that periodontopathic bacteria exert potent proinflammatory effects in human decidua. | 2010 | 20452492 |
| communication: antimicrobial activity of smap28 with a targeting domain for porphyromonas gingivalis. | antibiotic therapy is often used with mechanical therapy to treat periodontal disease. however, complications associated with antibiotic use can occur. a 'bacteria-specific' targeted approach would eliminate some of these complications and kill specific periodontopathogens without harming the commensal bacteria. one such approach is to couple antimicrobial peptides to a ligand, pheromone, or antibody specific for the periodontopathogen, porphyromonas gingivalis. to assess the feasibility of this ... | 2010 | 20454638 |
| antibacterial effects of mdpb against anaerobes associated with endodontic infections. | to investigate the antibacterial effects of 12-methacryloyloxydodecylpyridinium bromide (mdpb), an antibacterial monomer synthesized by combining quaternary ammonium with a methacryloyl group, against three anaerobes associated with endodontic infections using planktonic and biofilm cells. | 2010 | 20456514 |