Publications

atp synthesis by the (na+ + k+)-atpase in the absence of an ionic gradient. effects of organic solvent.	a 200-fold decrease in the pi concentration required for half-maximal phosphorylation of the (na+ + k+)-atpase is observed when 40% (v/v) dimethyl sulfoxide is added to the assay medium. the phosphoenzyme formed in the presence of dimethyl sulfoxide is able to transfer its phosphate to to form atp when concentrations of organic solvent and of na+, and is inhibited by ouabain.	1987	2820794
[anderson-fabry disease. current status. apropos of a case].	after submitting a typical case of anderson-fabry disease in a 38 years old male, we discuss the eminent aspects of this metabolic disorder and, above all, the clinical, biochemical and ultrastructural problems of diagnosis in the heterozygotic relatives.	2009	2821332
[anderson-fabry disease. current status. apropos of a case].	after submitting a typical case of anderson-fabry disease in a 38 years old male, we discuss the eminent aspects of this metabolic disorder and, above all, the clinical, biochemical and ultrastructural problems of diagnosis in the heterozygotic relatives.	2009	2821332
frequency of is1-mediated molecular events in different members of the family enterobacteriaceae.	the numbers of chromosomal copies of the insertion sequence is1 in strains of salmonella typhimurium (0 to 8 copies), shigella sonnei (56 copies), and shigella flexneri (41 copies) isolated in mexico city, mexico, were similar to those reported for these genera isolated in other countries. of the 11 shigella strains studied, all carried several small plasmids; however, in only one of these strains did a small plasmid contain is1, is1 recombination, cointegrate formation mediated by is1 or by the ...	1987	2822657
induction of phr gene expression by irradiation of ultraviolet light in escherichia coli.	to measure the degree of phr gene induction by dna-damaging agents, the promoter region was fused to the coding region of the lacz gene in plasmid pmc1403. the new plasmids were introduced into escherichia coli cells having different repair capabilities. more efficient induction of phr gene expression was detected in a uvra- strain as compared with the wild-type strain. in addition, obvious induction was detected in uvra- cells treated by 4-nitroquinoline 1-oxide and mitomycin c. nalidixic acid, ...	1987	2823069
induction of phr gene expression by irradiation of ultraviolet light in escherichia coli.	to measure the degree of phr gene induction by dna-damaging agents, the promoter region was fused to the coding region of the lacz gene in plasmid pmc1403. the new plasmids were introduced into escherichia coli cells having different repair capabilities. more efficient induction of phr gene expression was detected in a uvra- strain as compared with the wild-type strain. in addition, obvious induction was detected in uvra- cells treated by 4-nitroquinoline 1-oxide and mitomycin c. nalidixic acid, ...	1987	2823069
morphological differentiation of cultured astrocytes is blocked by cadmium or cobalt.	the morphological transformation of astrocytes in primary culture from flat to process bearing is induced by cyclic adenosine monophosphate (camp). calcium currents in cultured astrocytes are also enhanced by camp. this correlation suggests the hypothesis that camp-induced calcium entry causes the morphological differentiation. the experiments reported here show that cadmium or cobalt, which block astrocyte calcium channels, also block the camp-induced morphological differentiation. these result ...	1987	2823972
isolation and characterization of is elements repeated in the bacterial chromosome.	shigella sonnei contains repetitive sequences, including an insertion element is1, which can be isolated as double-stranded dna fragments by dna denaturation and renaturation and by treatment with s1 nuclease. in this paper, we describe a method of cloning the is1 fragments prepared by the s1 nuclease digestion technique into phage m13mp8 rfi dna. several clones contained is1, usually with a few additional bases. we isolated and characterized five other repetitive sequences using this method. on ...	1987	2824781
effects of palindrome size and sequence on genetic stability in the bacteriophage phi x174 genome.	by inserting palindromes of varying length and sequence into a non-essential region of the bacteriophage phi x174 genome we have investigated the effect of palindrome size and sequence on their genetic stability. multimers of increasing size of the ecori linker ccgaattcgg (e), the bamhi linker ccggatccgg (b) or mixtures of both (e, b) were inserted into the pvuii site of a previously constructed bacteriophage strain phi x174 j-f ins6. the largest inserts that could be maintained in the genome wi ...	1987	2824789
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.	selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...	1987	2825926
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.	selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...	1987	2825926
genetic and physical characterization of trimethoprim resistance plasmids from shigella sonnei and shigella flexneri.	analysis of six shigella flexneri and four s. sonnei isolates with trimethoprim (tp) resistance from clinical cases in ontario has shown that, in all isolates, the tp resistance is mediated by gene(s) on conjugative, multiple antibiotic-resistance plasmids. the physical and genetic characterization of these plasmids revealed that there are three different tp resistance plasmids. one group, composed of all six s. flexneri plasmids, consists of plasmids which are about 70 megadaltons (mda) and inh ...	1987	2825949
murine cytomegalovirus-pseudomonas synergistic infections: comparison of virulent and attenuated virus.	the synergistic interactions between pseudomonas aeruginosa and virulent or attenuated murine cytomegalovirus (mcmv) were compared in vivo. virulent mcmv challenge at a dose of 5 x 10(5) pfu/mouse intraperitoneally, followed by intranasal superinfection with 5 x 10(6) cfu/mouse of pseudomonas aeruginosa after 48 h resulted in greater than 80% mortality, apparently owing to a failure of pulmonary clearance mechanisms. single infections, or the use of attenuated mcmv in synergistic infections, did ...	1987	2825950
focal pontine leukoencephalopathy in a patient with the shwachman-diamond syndrome.	a 35-year-old woman who died after a long history of an illness consistent with shwachman-diamond syndrome, was found to have extensive calcified necrotizing lesions confined to the pontocerebellar fibers of the basis pontis. the possible relationship of this recently described lesion to the patient's immunosuppressed state and/or other systemic metabolic disturbances is discussed.	2009	2825956
focal pontine leukoencephalopathy in a patient with the shwachman-diamond syndrome.	a 35-year-old woman who died after a long history of an illness consistent with shwachman-diamond syndrome, was found to have extensive calcified necrotizing lesions confined to the pontocerebellar fibers of the basis pontis. the possible relationship of this recently described lesion to the patient's immunosuppressed state and/or other systemic metabolic disturbances is discussed.	2009	2825956
[reflections on 851 open heart operations at the institute of cardiology in abidjan].		2010	2826086
limb specific connections of the cat magnocellular red nucleus.	afferent and efferent connections of the limb specific divisions of the cat magnocellular red nucleus (rnm) were traced using the bidirectional transport of wheatgerm agglutinin-horseradish peroxidase complex (wga-hrp). injection sites within forelimb or hindlimb rnm regions were identified by microelectrode recording and confirmed by the position of labeled rubrospinal terminals. additional injections into structures that project to, or receive input from, rnm confirmed the somatotopic organiza ...	1987	2826551
metabolic studies of a sustained galactose overload in rat.	in order to obtain an experimental model for studying inherited disorders of galactose metabolism in man, we administered to adult rats a diet supplemented with 50% of galactose during 38 days. the results showed clinical symptoms such as polyuria, polydipsia, growth retardation and bilateral cataract. the main biochemical features were an increase in the galactokinase and uridyltransferase activities in liver, an ubiquitous accumulation in tissues and a considerable urinary elimination of galac ...	1987	2827561
synthesis and biological activity of n-methylated analogues of neurokinin a.	limited conformational constraints have been introduced in the sequence of the c-terminal heptapeptide nka(4-10) by n-methylation of individual peptide bonds and the biological activities of the peptides thus obtained were evaluated on three in vitro preparations sensitive and quite selective for each of the naturally-occurring neurokinins, in order to assess the effect of n-methylation on receptor selectivity. the analogue methylated in position 7, namely [meval7] nka(4-10) shows some selectivi ...	1987	2829053
selective effects of enzyme treatment used for dissociating single cells on their gaba-receptor activities.	frog dorsal root ganglion (drg) cells treated with collagenase and trypsin were dissociated mechanically. the characteristics of dose-response curves of gaba-induced cl- currents (icl) were mainly studied using the "concentration-clamp" technique, by which the external solution around isolated single neurons was rapidly changed within a few milliseconds. the saturating maximum currents induced by various concentrations of gaba normalized to the peak icl induced by 10(-5) m gaba on the same neuro ...	2010	2829067
purification and characterization of phytase from cotyledons of germinating soybean seeds.	soybean phytase (myo-inositol-hexakisphosphate phosphohydrolase; ec 3.1.3.8) was purified from 10-day-old germinating cotyledons using a four-step purification scheme. phytase was separable from the major acid phosphatase present, and stained as a minor band of the three acid phosphatases detectable by activity staining after gel electrophoresis. the purified enzyme exhibited two closely migrating bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of approximately 59 and 60 kda. ...	2009	2829733
purification and characterization of phytase from cotyledons of germinating soybean seeds.	soybean phytase (myo-inositol-hexakisphosphate phosphohydrolase; ec 3.1.3.8) was purified from 10-day-old germinating cotyledons using a four-step purification scheme. phytase was separable from the major acid phosphatase present, and stained as a minor band of the three acid phosphatases detectable by activity staining after gel electrophoresis. the purified enzyme exhibited two closely migrating bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of approximately 59 and 60 kda. ...	2009	2829733
identification of tonin in brain and exocrine tissues and in the cell-free translation products encoded by the mrna of these tissues.	tissue-specific expression of the esteropeptidase tonin [ec 3.4.99.-] was investigated in rat brain, submandibular gland, pancreas and kidney. specific polyclonal and monoclonal antibodies to purified rat tonin from the submandibular gland have been developed and characterized and have been purified via a tonin-agarose affinity column. immunoreactive tonin was measured by a recently developed tonin direct radioimmunoassay using a rabbit tonin antiserum. resulting tonin levels were found to be 10 ...	1987	2829829
liposomes as membrane model for study of lipid peroxidation.	this article describes the properties, production and characterization of liposomes with special reference to their use as membrane model for the study of lipid peroxidation. it presents briefly the methods that can be used for the assay of liposomal lipid peroxidation and brings out the special advantages these liposomes provide in elucidating the mechanism of lipid peroxidation by different physical and chemical agents. studies involving liposomal lipid peroxidation by different agents and the ...	2010	2830175
cytomegalovirus infection in burn patients.	we studied the seroepidemiology of cytomegalovirus (cmv) infection in 59 patients with thermal injuries. among the 31 patients who were initially cmv seronegative, seven (22.5%) seroconverted. patients who seroconverted had longer hospital stays (mean, 27 +/- 23.9 days v 14.9 +/- 8.8 days in seronegative patients [p = 0.03]) and they received more blood products (mean, 10.7 +/- 17.9 units v 1.8 +/- 2.8 units [p = 0.02]) than patients who remained seronegative. among 18 patients who were initiall ...	1987	2830289
development and testing of invasion-associated dna probes for detection of shigella spp. and enteroinvasive escherichia coli.	genetic determinants of the invasive phenotype of shigella spp. and enteroinvasive escherichia coli (eiec), two common agents of bacillary dysentery, are encoded on large (180- to 210 kilobase), nonconjugative plasmids. several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate the attachment and invasion of colonic epithelial cells by the bacteria. selected invasion plasmid antigen (ipa) genes have recently been cloned from shigella flexneri serotype 5 int ...	1988	2830310
sodium absorption.		2010	2830324
aids out of the closet.		2010	2830567
three distinct forms of type 2a protein phosphatase in human erythrocyte cytosol.	two type 2a protein phosphatases, phosphatases i (mr = 180,000) and iii (mr = 177,000), were purified to near homogeneity from human erythrocyte cytosol. phosphatase i was composed of alpha (34 kda), beta (63 kda), and delta (74 kda) subunits in a ratio of 1:1:1. phosphatase iii comprised alpha, beta, and gamma (53 kda) subunits in the same ratio. heparin-sepharose column chromatography converted most of phosphatase i and 20% of phosphatase iii into alpha 1 beta 1 which were indistinguishable fr ...	1988	2831201
apolipoprotein e-rich hdl binding to liver plasma membranes in copper-deficient rats.	copper deficiency in rats raises plasma cholesterol concentration while reducing live cholesterol concentration. one consequence of this cholesterol redistribution is the accumulation of a large high-density lipoprotein (hdl) particle rich in apolipoprotein e (apo e). the purpose of this study was to determine, using an in vitro binding assay, if the interaction of apo e-rich hdl with hepatic lipoprotein binding sites may be affected by copper deficiency. male sprague-dawley rats were divided in ...	1988	2831541
nmr contrast agents for proton imaging.		2010	2832350
flanking and internal regions of chromosomal genes mediating aerobactin iron uptake systems in enteroinvasive escherichia coli and shigella flexneri.	we have investigated the presence of the aerobactin system and the location of the aerobactin genes in enteroinvasive strains of escherichia coli. also, we cloned the aerobactin region and its flanking sequences from the chromosome of a strain of shigella flexneri and compared the molecular organization of the aerobactin genes in the two genera. of the 11 enteroinvasive e. coli strains studied, 5 possessed the aerobactin genes, which were located on the chromosome in each case. these strains pro ...	1987	2832521
analysis and isolation of cytomegalovirus dna by field inversion gel electrophoresis.	high molecular weight human cytomegalovirus (cmv) dna was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. a high yield of cmv dna molecules was obtained within 1 week of infecting the cell culture. labelling of the viral dna with biotin by nick translation enabled the detection of cmv-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.	1988	2832532
analysis and isolation of cytomegalovirus dna by field inversion gel electrophoresis.	high molecular weight human cytomegalovirus (cmv) dna was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. a high yield of cmv dna molecules was obtained within 1 week of infecting the cell culture. labelling of the viral dna with biotin by nick translation enabled the detection of cmv-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.	1988	2832532
continuous infusion of etoposide, bolus administration of cisplatin, and simultaneous radiation therapy in previously treated patients with small cell bronchogenic carcinoma.	thirty-one patients with previously treated small cell bronchogenic carcinoma were treated with split-course radiation therapy and concurrent cisplatin and etoposide. twenty-four patients (78%) had clinical partial or complete responses. median survival from the time of salvage was 6.3 months. fifty-eight percent of the patients failed on therapy initially in sites outside the radiation port. hematologic toxicity was moderate. concurrent cisplatin, etoposide, and split-course radiation therapy i ...	2010	2832766
viruses in sewage waters during and after a poliomyelitis outbreak and subsequent nationwide oral poliovirus vaccination campaign in finland.	during an outbreak of paralytic poliomyelitis in finland in 1984 and 1985 the widespread circulation of the causative wild-type serotype 3 poliovirus in the population was documented by demonstrating the virus in sewage water specimens in 13 different locations in the greater helsinki district and in 13 other cities or towns all over the country. after the nationwide campaign with oral poliovirus vaccine in 1985, poliovirus serotypes 2 and 3 were readily isolated from sewage waters for up to 2 m ...	1988	2833160
monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups. brief report.	isolates of bovine viral diarrhea (bvd) virus were differentiated by monoclonal antibodies (moabs) reactive with the 56 kd viral polypeptide. patterns of neutralizing activity of the moabs indicate that multiple epitopes are involved in virus neutralization.	1988	2833200
recovery of functional dna inserts by electroendosmotic elution during gel electrophoresis.	in contrast to all previous preparative electrophoresis apparatus which used a pump, electroendosmotic elution uses bound electrical charges at the end of the separating gel to generate a buffer flow. the electroendosmotic flow increased with increasing currents and decreasing buffer concentrations: its exact characteristics for the built apparatus were determined. the electroendosmotic device was able to separate two dna fragments differing in size by only 5% with a recovery over 95%. as demons ...	2004	2833722
"sub-threshold neoplastic states" created in transgenic mice.	several possible scenarios are already suggested by the first series of studies in which a high risk situation with respect to malignant diseases was artificially created by introducing oncogenes in the mouse germ line. only in exceptional cases was the expression of the transgene followed in an obligatory way by cancerous proliferation. the usual situation appears now to be that somatic cells expressing the transgene have only a higher probability than normal cells of undergoing a subsequent tr ...	1987	2835726
"sub-threshold neoplastic states" created in transgenic mice.	several possible scenarios are already suggested by the first series of studies in which a high risk situation with respect to malignant diseases was artificially created by introducing oncogenes in the mouse germ line. only in exceptional cases was the expression of the transgene followed in an obligatory way by cancerous proliferation. the usual situation appears now to be that somatic cells expressing the transgene have only a higher probability than normal cells of undergoing a subsequent tr ...	1987	2835726
virulence-associated genetic regions comprising 31 kilobases of the 230-kilobase plasmid in shigella flexneri 2a.	by random transposon tn5 insertions, we previously identified six virulence-associated sali fragments, b, d, f, g, h, and p, in the 230-kilobase plasmid pmysh6000 of shigella flexneri 2a. in this study, we analyzed the sites of 134 independent tn5 insertions on four contiguous sali fragments, b, p, h, and d, of pmysh6000 and identified five virulence-associated regions; four were associated with inducing a positive sereny test (ser), invasion into epithelial cells (inv), binding to congo red (pc ...	1988	2836357
molecular basis of sindbis virus neurovirulence in mice.	we examined a variety of strains of sindbis virus for the genetic changes responsible for differences in neurovirulence in mice. sv1a (a low passage of the ar339 strain of sindbis virus), a neuroadapted sindbis virus (nsv), and two laboratory strains of sindbis virus (hrsp and toto1101) were examined. nsv causes severe encephalomyelitis with hind-limb paralysis and high mortality after intracerebral inoculation in weanling mice. in contrast, sv1a causes only mild, nonfatal disease in weanling mi ...	1988	2836615
[cyclophosphamide-induced pneumonitis].	pneumotoxicity of cyclophosphamide is a rare complication of chemotherapy with this drug. 28 cases have so far been reported in world literature. we add one case in whom lung changes had appeared subacutely, after a single dose of cyclophosphamide, and were reversed by corticoids.	2009	2836905
[biochemical aspect in cardiac ischemia--ischemic changes and reperfusion injury].		2010	2838873
development of an auxotrophic oral live shigella flexneri vaccine.	an oral live attenuated shigella flexneri vaccine candidate strain was constructed by making it auxotrophic and dependent on aromatic metabolites not available in mammalian tissues. an arod gene of escherichia coli k12 strain nk 5131, inactivated by insertion in it of the tn 10 transposon, was transduced using phage p1 into a virulent s. flexneri serotype y strain (sfl 1) isolated from a patient with bacillary dysentery. one of the transductant strains sfl 114 was found to invade hela cells in v ...	1988	2838986
a variant of mucolipidosis. ii. clinical, biochemical and pathological investigations.	we present in this paper a patient with a clinically intermediate form of mucolipidosis (ml). lysosomal hydrolase activity in fibroblasts was normal and levels of these enzymes in culture media were not elevated. there was a striking elevation of several hydrolases in serum and a deficiency (15% of normal) of n-acetyl-glucosamine phosphotransferase in fibroblasts. atypical electron microscopic findings were also observed. there was no evidence of increased synthesis, slower turnover, unbalanced ...	2010	2839346
the structure of the human skin fibroblast collagenase gene.	genomic clones containing the complete gene encoding human fibroblast interstitial collagenase were isolated from a lambda phage human dna library. the gene is comprised from 10 exons and spans 8.2 kilobase pairs. we have mapped the relative positions and determined the dna sequence of all the exon/intron borders of the gene. the organization of the human interstitial collagenase gene is very similar to that of rabbit collagenase and of two other extracellular matrix (ecm) metalloproteases: rat ...	1988	2839503
the structure of the human skin fibroblast collagenase gene.	genomic clones containing the complete gene encoding human fibroblast interstitial collagenase were isolated from a lambda phage human dna library. the gene is comprised from 10 exons and spans 8.2 kilobase pairs. we have mapped the relative positions and determined the dna sequence of all the exon/intron borders of the gene. the organization of the human interstitial collagenase gene is very similar to that of rabbit collagenase and of two other extracellular matrix (ecm) metalloproteases: rat ...	1988	2839503
determination of pyrimidine 5'-nucleotidase (p5n) activity in whole blood as an index of lead exposure.	a simple method for determining pyrimidine 5'-nucleotidase (p5n) activity in whole blood has been developed, inhibiting the plasma activity for ump-hydrolysis by concanavalin (con a). con a specifically inhibits the activity of plasma 5'-nucleotidase (5n) but does not affect erythrocyte p5n activity. the anticoagulant edta partially inhibits 5n activity but slightly activates p5n. p5n activity determined by the present method with heparinised blood and con a was comparable with that by the metho ...	2010	2840110
investigation of the substrate binding and phosphate transfer in adenylate kinase by molecular mechanics.	molecular mechanics calculations of adenylate kinase (adk) complexed with atp, amp, and mg2+ have been carried out with three different model-built geometries. the lowest energy structure is that inferred from the x-ray structure, but such a geometry is not as consistent with the inference of an noe contact between his36 and the atp c2-h proton as the model of the second lowest energy. we have used the latter structure as a basis for modeling the reaction amp + atp----2adp in the presence of mg2 ...	2010	2840275
bspmii and acciii are an isoschizomer pair which differ in their sensitivity to cytosine methylation.		2010	2841649
cloned mouse lymphocytes permit analysis of somatic mutations that occur in vivo.	as part of our mouse model of somatic mutation, we have begun to characterize spontaneously occurring hypoxanthine phosphoribosyltransferase (hprt) -deficient mouse lymphocytes. lymphocytes were cloned by in vitro exposure of spleen cells from male c57b1/6 mice to the mitogen concanavalin a, conditioned medium containing lymphocyte growth factors, and thioguanine (tg), in a limiting dilution assay. the 17 tg-resistant clones recovered were all highly deficient in hprt activity and were found by ...	1987	2842875
selective induction of chromosomal gene expression by human cytomegalovirus.	during permissive infection, human cytomegalovirus (hcmv) increases the activities of certain cellular enzymes. in this report, we show that induction of these cellular genes occurs at the level of rna. the abundance of several additional growth-regulated cellular transcripts in hcmv-infected human foreskin fibroblasts (hff) was also compared to that in serum-stimulated hff cells. hcmv infection of hff cells resulted in increased abundance of ornithine decarboxylase, thymidine kinase, heat-shock ...	1988	2842946
detection of leukotriene b4 in cardiac tissue and its role in infarct extension through leucocyte migration.	the main left coronary artery of rats was ligated near its origin under light ether anaesthesia and the infarction observed for 48 h. the ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride. the unstained area (true ischaemic area) decreased with time to 27.1% of the left ventricle at 48 h, whereas the intensely stained area between the normal and the true ischaemic areas increased with time, sugges ...	1988	2844405
prenatal diazepam exposure affects beta-adrenergic receptors in brain regions of adult rat offspring.	the postnatal development of [3h]dihydroalprenolol binding to beta-adrenergic receptors has been studied in frontal cortex, cerebellum, striatum, and hypothalamus of the rat after prenatal and perinatal exposure to diazepam. dams were injected subcutaneously with single daily doses of 1 mg of diazepam/kg from day 7 to 20 of gestation or from day 15 of gestation to day 6 after birth. prenatal exposure had no effect on litter size or length of gestation or on the postnatal development of body and ...	1988	2844991
[partial 21-hydroxylase deficiency: study of 5 cases].		2010	2845201
effects of light- and dark-rearing on the postnatal development of gaba receptor sites in the chick optic lobe.	to investigate the ability of gaba receptor sites to undergo environmental-dependent plastic changes, the postnatal developmental pattern of gaba receptors was studied under different levels of light stimulation, i.e. normal-, light- and dark-rearing. at hatching the specific binding of [3h]gaba was 1.74 +/- 0.36 pmol/optic lobe. in normally reared chicks the number of gaba binding sites showed a transient increase with the highest value at the 6th day (7.0 +/- 1.32 pmol/optic lobe). this value ...	1987	2845719
[bovine herpesvirus type 2 infection].		1988	2845879
localization of plasmid loci necessary for the entry of shigella flexneri into hela cells, and characterization of one locus encoding four immunogenic polypeptides.	we have previously cloned a 44 kb fragment from the virulence plasmid of shigella flexneri serotype 5 strain m90t which is capable of restoring invasiveness to an avirulent, plasmidless mutant. this report presents a genetic and physical analysis of tn5 mutations in recombinant clone phs4108. tn5 mutagenesis allowed identification of at least five regions implicated in the entry phenotype. these regions were located on a 20 kb portion of phs4108. expression of the insertion mutants was studied b ...	1987	2846749
metabolic events mediating early killing of host cells infected by shigella flexneri.	j774, a continuous macrophage cell-line, was infected by m90t, an invasive isolate of shigella flexneri serotype 5 and bs176, its non invasive derivative--which does not harbor the 220 kbase virulence plasmid pwr100. killing of host cells by intracellular m90t, commenced one hour after infection and was completed by 4 hours. intracellular bs176 did not kill cells during the same period. cell protein biosynthesis was totally inhibited by both strains within 2 hours of infection thus indicating th ...	1987	2848171
interaction between bambuterol and physostigmine: aspects on cholinesterase inhibition and neuromuscular transmission in the smooth and skeletal muscles of the guinea-pig.	bambuterol, the bis-dimethyl carbamate prodrug of terbutaline, and physostigmine were examined with respect to their ability to interfere with the neuromuscular transmission in an isolated vagus nerve-trachea preparation, a phrenic nerve-diaphragm preparation and the transmurally stimulated extensor digotorum longus (edl) isolated from the guinea-pig. physostigmine increased the contractile response of the trachea to stimulation of the vagus nerve. bambuterol had an opposite effect in this respe ...	1988	2848230
comparisons of the pathogenicity of long and short fibres of chrysotile asbestos in rats.	long-term inhalation and intraperitoneal injection studies were undertaken with laboratory rats treated with a specially prepared short-fibre sample of canadian chrysotile asbestos. this was compared, at an equal mass dose, to dust generated from the same chrysotile batch so as to contain the highest possible number of long fibres. the long-fibre cloud contained roughly five times more fibres greater than 5 micron in length as seen by phase contrast optical microscopy (pcom). for increasing leng ...	1988	2848570
expression of env sequences of the bovine leukemia virus (blv) in the yeast saccharomyces cerevisiae.	dna sequences of the envelope (env) gene of the bovine leukemia virus (blv) were expressed in the yeast saccharomyces cerevisiae. two yeast promoters, the repressible pho5 promoter and the constitutive pgk promoter, were used to construct four expression plasmids comprising either a sequence of the surface antigen gp51 or a (gp51 + gp30) sequence. the expressed heterologous gene products were characterized by western blot analysis and competitive radioimmunoassay. by means of northern blot analy ...	1988	2849260
peripheral neuropathy associated with mitochondrial myopathy.	twenty patients with mitochondrial myopathy were investigated for the presence of peripheral neuropathy. there were clinical features of a mild sensorimotor neuropathy in five patients (25%) and nerve conduction studies were abnormal in 10 patients (50%). electrophysiological studies of the whole group showed significant impairment of motor and sensory conduction compared with controls. sural nerve biopsy and morphometric studies were performed on four patients with clinical neuropathy. there wa ...	2010	2849395
peripheral neuropathy in elderly cba mice.	a late-onset peripheral neuropathy in a mouse with a wider progressive neurological disorder is morphologically characterized for comparison with late life human neuropathies. preliminary studies indicate the presence of a proximal, predominantly motor, axonopathy in the mouse model.	1988	2849396
salience and the effects of cs preexposure on aversion conditioning.	rats (n = 84) received preexposure to distilled water or to one of two differently salient flavors, 5.0% casein or 10.0% sucrose, casein being the more salient. each preexposure group then received aversion conditioning to a 5.0% casein or a 10.0% sucrose cs. aversion effects were reliably more enduring to casein than to sucrose. relative to water-preexposed groups, preexposure to casein attenuated aversion effects to the casein cs reliably less than preexposure to sucrose attenuated aversion ef ...	1988	2849411
similarity between human retinoic acid receptor and escherichia coli homoserine kinase.		1988	2849427
a new class of free radical scavengers reducing adriamycin mitochondrial toxicity.	beef heart mitochondria were incubated with adm and nadh. an adriamycin semiquinone radical was detected using esr spectroscopy. the semiquinone radical production rate is decreased upon addition of a scavenger (ad 20) in the reaction medium. nmri mice were treated with ad 20 (70 mg/kg, i.p.) 15 min prior adm injection (20 mg/kg, i.p.) or with adm alone. heart mitochondria were isolated 48 hr later. the enzymatic activities of complex i-iii and complex iv of the mitochondrial respiratory chain w ...	1988	2849451
the cupric site in nitrous oxide reductase contains a mixed-valence [cu(ii),cu(i)] binuclear center: a multifrequency electron paramagnetic resonance investigation.	multifrequency electron paramagnetic resonance (epr) spectra of the cu(ii) site in nitrous oxide reductase (n2or) from pseudomonas stutzeri confirm the assignment of the low field g value at 2.18 consistent with the seven line pattern observed at 9.31 ghz, 10 k. s-band spectra at 20 k are better resolved than the x-band spectra recorded at 10 k. the features observed at 2.4, 3.4, 9.31 and 35 ghz are explained by a mixed-valence [cu(1.5)..cu(1.5)] s = 1/2 species with the unpaired electron deloca ...	1988	2849565
malignant fibrous histiocytoma in keratosis, ichthyosis, and deafness syndrome.		2010	2849613
the spectrum and significance of bronchioloalveolar carcinomas.		2009	2849745
human rotavirus infection enhances invasiveness of enterobacteria in ma-104 cells.	to study the interaction between common pathogens causing infectious diarrhea in humans, ma 104 cell cultures were infected with human rotavirus and salmonella typhimurium or shigella flexneri or enteroinvasive escherichia coli (eiec) concomitantly. when ma-104 cells were preinfected with human rotavirus, invasiveness of s. typhimurium was significantly enhanced. the enhancement was evident after 48 h of virus preincubation. at this time virus specific antigens were demonstrated in the cell cult ...	1988	2850823
transfer of pre-embryos donated and fertilized in vivo to recipients given a steroid replacement therapy.	this study describes eleven procedures of non-surgical recovery of preimplantation conceptuses from the uteri of eight fertile donor women and the transfer of the recovered pre-embryos into the uteri of infertile recipients with primary ovarian failure, or with hypothalamic--pituitary failure. conjugated oestrogens and progesterone were administered to recipients as replacement steroids during the first 20 weeks of pregnancy, in order to produce a secretory endometrium allowing nidation and deve ...	2010	2851614
neuron-specific enolase in the diagnosis of small-cell lung cancer with pleural effusion: a negative report.	we measured the concentration of neuron-specific enolase (nse) and carcinoembryonic antigen (cea) in the serum and pleural fluid of 53 patients with pleural effusions, including seven patients with small-cell lung cancer (sclc). high levels (above 12.5 micrograms.l-1) of nse in pleural fluid were observed in five patients with sclc (sensitivity 71%). however, pleural fluid nse levels were also increased in five patients with other types of cancer and in four patients with non-malignant inflammat ...	2010	2853080
endotoxin-induced uveitis in rats: morphological and biochemical study.	inflammation induced by systemic injection of endotoxin can be a good model for endogenous uveitis since ocular inflammation is induced without manipulating the eye. we carried out morphological and biochemical studies in lewis rats to evaluate the breakdown of the blood-ocular barrier following injection of endotoxin (1 mg/rat) in footpads. vasodilation was observed as early as 3 hours and became maximum at 18-24 hours after the injection. unlike in the eye, no inflammatory changes were observe ...	1988	2853240
in vitro activity of azithromycin (cp-62,993), a novel macrolide, against enteric pathogens.	azithromycin (cp-62,993 [9-deoxy-9a-methyl-9a-aza-9a-homoerythromycin]) is a novel macrolide antimicrobial. in this study the in vitro activity of cp-62,993 has been determined against selected enteropathogens, including clostridium difficile, and compared with that of erythromycin. mics were determined using an agar incorporation technique in mueller-hinton medium, containing saponin-lysed horse blood at a final concentration of 10% v/v, with an inoculum of 10(4) cfu. cp-62,993 was considerably ...	1988	2854515
genetic analysis of the plasmid region controlling virulence in yersinia enterocolitica 0:9 by mini-mu insertions and lac gene fusions.	enteropathogenic strains of yersinia enterocolitica harbor a virulence plasmid (pyve plasmid) of 70 kilobases (kb) which specifies, at 37 degrees c, a calcium requirement for growth, autoagglutinability, resistance to the bactericidal activity of human serum, and the expression of outer membrane proteins (omp). some mutations suppress the calcium requirement for growth while others make the bacteria unable to grow at 37 degrees c, even in the presence of calcium. to analyse the genes involved in ...	1986	2854600
rural health care in the developing world.		2010	2857377
striatal synaptosomal dopamine synthesis: evidence against direct regulation by an autoreceptor mechanism.	regulation of the rate-limiting step in dopamine (da) synthesis was estimated in striatal synaptosomes by measuring the rate of hydroxylation of l-4-[3h]phenylalanine, a substrate of tyrosine hydroxylase (th). da inhibited hydroxylation with an ic50 of 0.2 microm. the concentration-response curve of da-induced inhibition was not affected by the presence of 1 microm chlorpromazine, a phenothiazine da antagonist. sulpiride and haloperidol, da antagonists of the benzamide and butyrophenone classes ...	1985	2859213
prolactin and its involvement in fluid regulation in the bovine.	plasma prolactin (prl) concentrations, osmolality, water consumption, feed intake, urine excretion, and fecal water output were determined in twelve steers of 3 breeds exposed to 5 feed and water regimes. breed differences were found in water intake and plasma prl concentrations when feed and water were ad lib., however, during any of the other 4 treatments, responses were similar between breeds. during dehydration and feed restriction, water intake, urine, fecal water, and plasma prolactin decr ...	1985	2859961
hazards of combined beta-blocker/diuretic tablets.		2005	2860426
morphological characterization of the somatostatin-immunoreactive dendritic skin cells in urticaria pigmentosa patients by computerized image analysis.	by means of computerized image analysis, the somatostatin-immunoreactive epidermal and dermal dendritic cells of certain urticaria pigmentosa patients were characterized. the evidence obtained supported the concept that the epidermal cells correspond to the langerhans cells.	2009	2860719
morphological characterization of the somatostatin-immunoreactive dendritic skin cells in urticaria pigmentosa patients by computerized image analysis.	by means of computerized image analysis, the somatostatin-immunoreactive epidermal and dermal dendritic cells of certain urticaria pigmentosa patients were characterized. the evidence obtained supported the concept that the epidermal cells correspond to the langerhans cells.	2009	2860719
effects of excitatory amino acids on calcium transport by brain membranes.	the effect of excitatory amino acids on the uptake of 45ca was studied in crude mitochondrial (p2) fractions prepared from mouse brain. l-glutamate stimulated calcium uptake, but this action was not shared by other amino acids including d-glutamate, l-aspartate, n-methyl-aspartate or kainate. the glutamate-stimulated calcium uptake was, however, blocked by inhibitors of glutamate dehydrogenase, such as d-glutamate, glutarate and triiodothyronine. subcellular fractionation demonstrated that the u ...	1985	2860953
chemotaxis by entamoeba histolytica.	a micropore membrane procedure to assay taxis by entamoeba histolytica is described and the results of studies of responses to a variety of soluble substances, bacteria, an rat colon washings using this procedure are reported. trophozoites migrated in blind well chambers through 8-micron pore size polycarbonate membranes but not nitrocellulose membranes up to 12 micron pore size. amoebae were attracted toward fresh axenic culture medium (tyi-s), an enzymatic hydrolysate of casein (trypticase), a ...	1985	2861283
[effect of signopam (polfa) on clinical picture and thyroid function in patients with hyperthyroidism and euthyroidism].		2010	2861696
antibodies to htlv-i p24 in african and portuguese populations.	using a radioimmunoassay to detect htlv-i protein antibodies of molecular weight 24,000, we screened populations from algeria (140 subjects), tunisia (442), mali (69), senegal (415), uganda (135), the central african republic (77), the congo (360), and madagascar (193). only four subjects were positive (1 from senegal, 1 from uganda, 2 from the congo). this is a much lower figure than that found by others in africa by the enzyme-linked immunosorbent assay technique. in addition, 319 portuguese b ...	1985	2861895
hazard of potassium chloride solution.		2010	2862489
transformation of primary cultured and co-cultured adult rat liver cells by 3'-methyl-4-dimethylaminoazobenzene.	under various conditions of culture and carcinogen treatment, the transformation of liver cells by 3'-methyl-4-dimethylaminoazobenzene (3'-me-dab) was studied. primary liver cell (plc) cultures from adult male rats and co-cultures with plcs of arl-d8 cells of a liver epithelial-like clear cell line from adult female rats were treated with 0.24 mm 3'-me-dab for 6 days. four of 8 carcinogen-treated plc cultures contained cells with marker chromosomes, and 3 of the 8 cultures contained gamma-glutam ...	1985	2862759
generation of electronically excited species during enzymatic oxidation of chlorpromazine and related compound.	chemiluminescence in visible region was detected, during peroxidase-catalyzed oxidation of chlorpromazine at ph7.5 (but not at ph4.25) or of propericiazine, both at ph7.5 and at ph4.25. red colored intermediates, cation radicals, were produced and decayed in all enzymatic systems used. chemiluminescence was also detected at ph7.5, but not at ph4.25, when synthesized cation radical of chlorpromazine in water was mixed with concentrated buffer. protonation of alkyl nitrogen in the cation radical o ...	2010	2862870
[relationship between hosts and hemorrhagic fever renal syndrome in chang-an county, shan-xi province].		1985	2862998
characterization of enhanced intestinal permeability; electrophysiological study on the effects of diclofenac and ethylenediaminetetraacetic acid.	the mechanism of the enhancing effects of diclofenac sodium and disodium ethylenediaminetetraacetate (edta) on intestinal permeability to sulphanilic acid were investigated in the rat isolated jejunum by an electrophysiological technique. the results suggested that diclofenac sodium increased the mucosal-to-serosal flux rate of sulphanilic acid by enhancing both transcellular and paracellular permeabilities while the effect of edta was located only in the paracellular pathway.	1985	2863363
domestic chlorine poisoning.		2010	2863507
possible role of insulin deficiency and growth hormone in severe retinopathy.		2010	2863521
practical importance of immunostaining paraffin-embedded biopsy samples of small lymphocytic and follicle centre cell lymphomas for s100 protein.		2010	2863627
iga response during accidental infection with shigella flexneri.		1985	2863666
combination of mefloquine with sulfadoxine-pyrimethamine compared with two sulfadoxine-pyrimethamine combinations in malaria chemoprophylaxis.		1985	2863679
snake bite in pregnancy.		2010	2863717
immunocytochemical studies of somatostatin neurons in brain.	immunohistochemical studies with antisera to somatostatin have, in many instances, led the way to our present understanding of the peptidergic nervous system. somatostatin was among the first of the hypophysiotropic hormones shown to be contained in diverse neuronal circuits outside of the hypothalamus. for example, somatostatin is found within neurons ranging in location from the cerebral cortex to primary sensory neurons to enteric neurons within the gut wall. somatostatin was also the first n ...	1985	2863929
distribution and morphology of somatostatin cells.		2010	2863943