Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| significant improvement of oxidase activity through the genetic incorporation of a redox-active unnatural amino acid. | while nature employs various covalent and non-covalent strategies to modulate tyrosine (y) redox potential and pka to optimize enzyme activities, such approaches have not been systematically applied for the design of functional metalloproteins. through the genetic incorporation of 3-methoxytyrosine (omey) into myoglobin, we recapitulated important features of cytochrome c oxidase (cco) into this small soluble protein, which exhibits selective o2 reduction activity while generating small amount o ... | 2015 | 26417427 |
| humans differ in their personal microbial cloud. | dispersal of microbes between humans and the built environment can occur through direct contact with surfaces or through airborne release; the latter mechanism remains poorly understood. humans emit upwards of 10(6) biological particles per hour, and have long been known to transmit pathogens to other individuals and to indoor surfaces. however it has not previously been demonstrated that humans emit a detectible microbial cloud into surrounding indoor air, nor whether such clouds are sufficient ... | 2015 | 26417541 |
| fluorescence polarization based nucleic acid testing for rapid and cost-effective diagnosis of infectious disease. | a new nucleic acid detection method was developed for a rapid and cost-effective diagnosis of infectious disease. this approach relies on the three unique elements: 1) detection probes that regulate dna polymerase activity in response to the complementary target dna; 2) universal reporters conjugated with a single fluorophore; and 3) fluorescence polarization (fp) detection. as a proof-of-concept, the assay was used to detect and sub-type salmonella bacteria with sensitivities down to a single b ... | 2015 | 26420633 |
| dissemination of extended-spectrum β-lactamases and quinolone resistance genes among clinical isolates of uropathogenic escherichia coli in children. | urinary tract infection (uti) is one of the most common childhood bacterial infections and escherichia coli is the major pathogen. producing β-lactamase enzymes are the most common mechanism of bacterial resistance. | 2015 | 26421128 |
| evaluation of the rapidec carba np test for detection of carbapenemases in enterobacteriaceae. | this study evaluated the performance of the rapidec carba np test, which was introduced recently into the market for the detection of carbapenemase production in a broad spectrum of β-lactamase-producing enterobacteriaceae clinical isolates. in total, 252 clinical enterobacteriaceae isolates that had been genetically characterized with respect to carbapenemase, extended-spectrum β-lactamase (esbl), and ampc genes were analyzed; 51/252 isolates (20.2%) were genetically confirmed to be carbapenema ... | 2015 | 26424840 |
| simplified protocol for carba np test for enhanced detection of carbapenemase producers directly from bacterial cultures. | we compared carbapenemase detection among 266 gram-negative bacilli (161 carbapenemase producers) using the carba np tests issued by the clsi (cnpt-clsi) and a novel protocol (cnpt-direct) designed for carbapenemase detection direct from bacterial cultures (instead of bacterial extracts required by the clsi tests). the specificities were comparable (100%), but the cnpt-direct was more sensitive (98% versus 84%). the cnpt-direct was easier to perform due to the direct use of colonies and offered ... | 2015 | 26424841 |
| complete genome sequence of citrobacter freundii myophage merlin. | bacteriophage merlin is a t4-like myophage which infects citrobacter freundii, a member of the enterobacteriaceae family. c. freundii is an opportunistic pathogen that is a common cause of nosocomial infections. this report announces the complete genome of myophage merlin and describes its features. | 2015 | 26430046 |
| complete genome sequence of citrobacter freundii myophage michonne. | citrobacter freundii is a gram-negative opportunistic pathogen that causes dangerous infections such as neonatal meningitis. c. freundii also harbors antibiotic resistance, making phages infecting this host valuable tools. here, we announce the complete genome of the c. freundii felixo1-like myophage michonne and describe its notable features. | 2015 | 26430047 |
| comparative evaluation of the hydrogen production by mixed consortium, synthetic co-culture and pure culture using distillery effluent. | wastewater comprises of various carbon sources. so, the use of microbial consortium may improve the hydrogen production and organic reduction. the present study deals with biohydrogen production by acidogenic mixed consortia (amc), synthetic co-culture (klebsiella pneumoniae iit-bt 08 and citrobacter freundii iit-bt l139) and pure culture using distillery effluent (de). higher hydrogen yield was observed in case of amc (9.17 mol/kg codreduced) as compared to the synthetic co-culture and pure cul ... | 2015 | 26433784 |
| aerobic bacteriological study of acute exacerbations of chronic obstructive pulmonary disease. | the natural history of chronic obstructive pulmonary disease is characterized by frequent exacerbations. majority of exacerbations are infectious and bacteria responsible for 30-50% of these cases. the purpose of this study was to determine the bacteriology of acute exacerbations of chronic obstructive pulmonary disease in hospitalized patients in our institution and their antibiotic susceptibility pattern to formulate cost effective antibiotic strategy and reducing the emergence of drug resista ... | 2015 | 26435942 |
| adverse events associated with fosfomycin use: review of the literature and analyses of the fda adverse event reporting system database. | the growing problem of antibacterial resistance resulted in an increased interest in fosfomycin, especially its parenteral formulation. we reviewed fosfomycin safety profile using the food and drug administration adverse event (ae) reporting system (faers) and published literature. | 2015 | 26437630 |
| increased hydrolysis of oximino-β-lactams by cmy-107, a tyr199cys mutant form of cmy-2 produced by escherichia coli. | the cephalosporinase cmy-107, a tyr199cys mutant form of cmy-2 encoded by an inci self-transferable plasmid carried by an escherichia coli clinical strain, was characterized. the enzyme hydrolyzed oximino-cephalosporins and aztreonam more efficiently than cmy-2 did. | 2015 | 26438499 |
| flow cytometric evaluation of physico-chemical impact on gram-positive and gram-negative bacteria. | since heat sensitivity of fruits and vegetables limits the application of thermal inactivation processes, new emerging inactivation technologies have to be established to fulfill the requirements of food safety without affecting the produce quality. the efficiency of inactivation treatments has to be ensured and monitored. monitoring of inactivation effects is commonly performed using traditional cultivation methods which have the disadvantage of the time span needed to obtain results. the aim o ... | 2015 | 26441874 |
| my life with mu. | 2015 | 26442190 | |
| surgical correction of rectal prolapse in laboratory mice (mus musculus). | rectal prolapse is a common clinical problem in laboratory mice. this condition may occur spontaneously, develop after genetic manipulations, result from infections with pathogens such as citrobacter species, or arise secondary to experimental design such as colitis models. the current standard of care at our institution is limited to monitoring mice until tissue becomes ulcerated or necrotic; this strategy often leads to premature euthanasia of valuable animals prior to the study endpoint. surg ... | 2015 | 26442289 |
| biotin and lipoic acid: synthesis, attachment, and regulation. | two vitamins, biotin and lipoic acid, are essential in all three domains of life. both coenzymes function only when covalently attached to key metabolic enzymes. there they act as "swinging arms" that shuttle intermediates between two active sites (= covalent substrate channeling) of key metabolic enzymes. although biotin was discovered over 100 years ago and lipoic acid 60 years ago, it was not known how either coenzyme is made until recently. in escherichia coli the synthetic pathways for both ... | 2014 | 26442940 |
| characterization of phicfp-1, a virulent bacteriophage specific for citrobacter freundii. | citrobacter freundii, a gram-negative bacterium, causes many opportunistic infections. bacteriophage phicfp-1 was isolated and characterized by its ability to lyse the multidrug-resistant clinical c. freundii strain p10159. transmission electron microscopy showed that the phage has an icosahedral head and a short tail, making it a podoviridae family member. in a single-step growth experiment, phicfp-1 exhibited an eclipse period of 20 min and a burst size of 100 particles per cell. its genome as ... | 2016 | 26455439 |
| cooccurrence of multiple ampc β-lactamases in escherichia coli, klebsiella pneumoniae, and proteus mirabilis in tunisia. | over a period of 40 months, plasmid-mediated ampc β-lactamases were detected in tunis, tunisia, in 78 isolates (0.59%) of escherichia coli, klebsiella pneumoniae, and proteus mirabilis. in 67 isolates, only one ampc gene was detected, i.e., blacmy-2-type (n = 33), blaacc (n = 23), bladha (n = 6) or blaebc (n = 5). multiple ampc genes were detected in 11 isolates, with the following distribution: blamox-2, blafox-3, and blacmy-4/16 (n = 6), blafox-3 and blamox-2 (n = 3), and blacmy-4 and blamox-2 ... | 2015 | 26459902 |
| cooccurrence of multiple ampc β-lactamases in escherichia coli, klebsiella pneumoniae, and proteus mirabilis in tunisia. | over a period of 40 months, plasmid-mediated ampc β-lactamases were detected in tunis, tunisia, in 78 isolates (0.59%) of escherichia coli, klebsiella pneumoniae, and proteus mirabilis. in 67 isolates, only one ampc gene was detected, i.e., blacmy-2-type (n = 33), blaacc (n = 23), bladha (n = 6) or blaebc (n = 5). multiple ampc genes were detected in 11 isolates, with the following distribution: blamox-2, blafox-3, and blacmy-4/16 (n = 6), blafox-3 and blamox-2 (n = 3), and blacmy-4 and blamox-2 ... | 2015 | 26459902 |
| metabolic engineering of klebsiella pneumoniae for the production of 2-butanone from glucose. | 2-butanone is an important commodity chemical of wide application in different areas. in this study, klebsiella pneumoniae was engineered to directly produce 2-butanone from glucose by extending its native 2, 3-butanediol synthesis pathway. to identify the potential enzyme for the efficient conversion of 2, 3-butanediol to 2-butanone, we screened different glycerol dehydratases and diol dehydratases. by introducing the diol dehydratase from lactobacillus brevis and deleting the ldha gene encodin ... | 2015 | 26465746 |
| high prevalence of salmonella and imp-4-producing enterobacteriaceae in the silver gull on five islands, australia. | the objective of this study was to investigate the silver gull as an indicator of environmental contamination by salmonellae and carbapenemase-producing enterobacteriaceae (cpe) in south-east australia. | 2015 | 26472769 |
| high prevalence of salmonella and imp-4-producing enterobacteriaceae in the silver gull on five islands, australia. | the objective of this study was to investigate the silver gull as an indicator of environmental contamination by salmonellae and carbapenemase-producing enterobacteriaceae (cpe) in south-east australia. | 2015 | 26472769 |
| complete genome sequence of citrobacter freundii myophage mordin. | citrobacter freundii is a gram-negative opportunistic pathogen that is associated with urinary tract infections. bacteriophages infecting c. freundii can be used as an effective treatment to fight these infections. here, we announce the complete genome sequence of the c. freundii felix o1-like myophage mordin and describe its features. | 2015 | 26472844 |
| molecular characterization of carbapenem-nonsusceptible enterobacterial isolates collected during a prospective interregional survey in france and susceptibility to the novel ceftazidime-avibactam and aztreonam-avibactam combinations. | an interregional surveillance program was conducted in the northwestern part of france to determine the prevalence of carbapenem-nonsusceptible enterobacteriaceae (cnse) isolates and their susceptibility to ceftazidime-avibactam and aztreonam-avibactam combinations. nonduplicate cnse clinical isolates were prospectively collected from six hospitals between june 2012 and november 2013. mics of ceftazidime and aztreonam, alone or combined with a fixed concentration of avibactam (4 μg/ml), and thos ... | 2015 | 26482307 |
| molecular characterization of carbapenem-nonsusceptible enterobacterial isolates collected during a prospective interregional survey in france and susceptibility to the novel ceftazidime-avibactam and aztreonam-avibactam combinations. | an interregional surveillance program was conducted in the northwestern part of france to determine the prevalence of carbapenem-nonsusceptible enterobacteriaceae (cnse) isolates and their susceptibility to ceftazidime-avibactam and aztreonam-avibactam combinations. nonduplicate cnse clinical isolates were prospectively collected from six hospitals between june 2012 and november 2013. mics of ceftazidime and aztreonam, alone or combined with a fixed concentration of avibactam (4 μg/ml), and thos ... | 2015 | 26482307 |
| antimicrobial susceptibilities of aerobic and facultative gram-negative bacilli from intra-abdominal infections in patients from seven regions in china in 2012 and 2013. | to evaluate the antimicrobial susceptibility of gram-negative bacilli that caused hospital-acquired and community-acquired intra-abdominal infections (iais) in china between 2012 and 2013, we determined the susceptibilities to 12 antimicrobials and the extended-spectrum β-lactamase (esbl) statuses of 3,540 iai isolates from seven geographic areas in china in a central laboratory using clsi broth microdilution and interpretive standards. most infections were caused by escherichia coli (46.3%) and ... | 2015 | 26482308 |
| antimicrobial susceptibilities of aerobic and facultative gram-negative bacilli from intra-abdominal infections in patients from seven regions in china in 2012 and 2013. | to evaluate the antimicrobial susceptibility of gram-negative bacilli that caused hospital-acquired and community-acquired intra-abdominal infections (iais) in china between 2012 and 2013, we determined the susceptibilities to 12 antimicrobials and the extended-spectrum β-lactamase (esbl) statuses of 3,540 iai isolates from seven geographic areas in china in a central laboratory using clsi broth microdilution and interpretive standards. most infections were caused by escherichia coli (46.3%) and ... | 2015 | 26482308 |
| relationship between intestinal microbiota and colorectal cancer. | the human gastrointestinal tract hosts a complex and vast microbial community with up to 10(11)-10(12) microorganisms colonizing the colon. the gut microbiota has a serious effect on homeostasis and pathogenesis through a number of mechanisms. in recent years, the relationship between the intestinal microbiota and sporadic colorectal cancer has attracted much scientific interest. mechanisms underlying colonic carcinogenesis include the conversion of procarcinogenic diet-related factors to carcin ... | 2015 | 26483877 |
| detection of carbapenemases in clinical enterobacteriaceae isolates using the vitek ast-n202 card. | the rapid and accurate detection of carbapenemase-producing enterobacteriaceae (cpe) in clinical microbiology laboratories is essential for the treatment and control of infections caused by these microorganisms. this study was performed to evaluate the ability of the vitek ast-n202 card to detect cpe isolates. | 2015 | 26483990 |
| in vitro antibacterial activity of doripenem against gram-negative blood isolates in a korean tertiary care center. | doripenem is the most recently introduced antimicrobial agent of the carbapenem class. it is a valuable therapeutic option in the context of increasing antimicrobial resistance to imipenem and meropenem among gram-negative bacilli (gnb) clinical isolates. however, clinicians are usually reluctant to prescribe doripenem, because susceptibility to doripenem is not automatically reported by most clinical laboratories and the in vitro activity of doripenem against clinically significant gnb isolates ... | 2015 | 26483991 |
| genome sequencing, annotation of citrobacter freundii strain gtc 09479. | we report the 4.9-mb genome sequence of citrobacter freundii strain gtc 09479, isolated from urine sample collected during the year 1983 at gifu university graduate school of medicine, japan. this draft genome consist of 4,899,578 bp with 51.62% g + c, 4,574 predicted cdss, 72 trnas and 10 rrnas. | 2014 | 26484065 |
| carbapenem-resistant enterobacteriaceae in children, united states, 1999-2012. | the prevalence of carbapenem-resistant enterobacteriaceae (cre) infections is increasing in the united states. however, few studies have addressed their epidemiology in children. to phenotypically identify cre isolates cultured from patients 1-17 years of age, we used antimicrobial susceptibilities of enterobacteriaceae reported to 300 laboratories participating in the surveillance network-usa database during january 1999-july 2012. of 316,253 isolates analyzed, 266 (0.08%) were identified as cr ... | 2015 | 26486124 |
| resistance integrons: class 1, 2 and 3 integrons. | as recently indiscriminate abuse of existing antibiotics in both clinical and veterinary treatment leads to proliferation of antibiotic resistance in microbes and poses a dilemma for the future treatment of such bacterial infection, antimicrobial resistance has been considered to be one of the currently leading concerns in global public health, and reported to widely spread and extended to a large variety of microorganisms. in china, as one of the currently worst areas for antibiotics abuse, the ... | 2015 | 26487554 |
| port site infection in laparoscopic surgery: a review of its management. | laparoscopic surgery (ls), also termed minimal access surgery, has brought a paradigm shift in the approach to modern surgical care. early postoperative recovery, less pain, improved aesthesis and early return to work have led to its popularity both amongst surgeons and patients. its application has progressed from cholecystectomies and appendectomies to various other fields including gastrointestinal surgery, urology, gynecology and oncosurgery. however, ls has its own package of complications. ... | 2015 | 26488021 |
| evaluation of an inexpensive growth medium for direct detection of escherichia coli in temperate and sub-tropical waters. | the cost and complexity of traditional methods for the detection of faecal indicator bacteria, including e. coli, hinder widespread monitoring of drinking water quality, especially in low-income countries and outside controlled laboratory settings. in these settings the problem is exacerbated by the lack of inexpensive media for the detection of e. coli in drinking water. we developed a new low-cost growth medium, aquatest (at), and validated its use for the direct detection of e. coli in temper ... | 2015 | 26495983 |
| pyogenic sacroiliitis in a 13-month-old child: a case report and literature review. | pyogenic sacroiliitis is exceptional in very young children. diagnosis is difficult because clinical examination is misleading. faber test is rarely helpful in very young children. inflammatory syndrome is frequent. bone scintigraphy and mri are very sensitive for the diagnosis. joint fluid aspiration and blood cultures are useful to identify the pathogen. appropriate antibiotic therapy provides rapid regression of symptoms and healing. we report the case of pyogenic sacroiliitis in a 13-month-o ... | 2015 | 26496260 |
| growth kinetics of salmonella enterica in hajna tetrathionate broth, rappaport broth and modified semisolid rappaport agar. | to determine the appropriate method for isolating salmonella enterica, we compared the growth of s. enterica serovars using three selective enrichment media. s. enterica was more successfully isolated from artificially contaminated fecal samples after enrichment in hajna tetrathionate broth or modified semisolid rappaport agar than in rappaport broth. since most bacteria (other than motile s. enterica) do not migrate on modified semisolid rappaport agar, the growth characteristics of s. enterica ... | 2015 | 26498402 |
| growth kinetics of salmonella enterica in hajna tetrathionate broth, rappaport broth and modified semisolid rappaport agar. | to determine the appropriate method for isolating salmonella enterica, we compared the growth of s. enterica serovars using three selective enrichment media. s. enterica was more successfully isolated from artificially contaminated fecal samples after enrichment in hajna tetrathionate broth or modified semisolid rappaport agar than in rappaport broth. since most bacteria (other than motile s. enterica) do not migrate on modified semisolid rappaport agar, the growth characteristics of s. enterica ... | 2015 | 26498402 |
| deterioration to extinction of wastewater bacteria by non-thermal atmospheric pressure air plasma as assessed by 16s rdna-dgge fingerprinting. | the use of cold plasma jets for inactivation of a variety of microorganisms has recently been evaluated via culture-based methods. accordingly, elucidation of the role of cold plasma in decontamination would be inaccurate because most microbial populations within a system remain unexplored owing to the high amount of yet uncultured bacteria. the impact of cold atmospheric plasma on the bacterial community structure of wastewater from two different industries was investigated by metagenomic-based ... | 2015 | 26500637 |
| optical scatter patterns facilitate rapid differentiation of enterobacteriaceae on chromagar™ orientation medium. | enterobacteriaceae family comprised pathogens and commensals and has a significant impact on food safety and public health. enterobacteriaceae is often enumerated and presumptively identified on chromogenic media, such as chromagar(tm) orientation medium based on colony profile; however, classification is highly arbitrary, and some could not be differentiated due to similar chromogen production. here, we investigated the ability of the laser optical sensor, bardot (bacterial rapid detection usin ... | 2015 | 26503189 |
| optical scatter patterns facilitate rapid differentiation of enterobacteriaceae on chromagar™ orientation medium. | enterobacteriaceae family comprised pathogens and commensals and has a significant impact on food safety and public health. enterobacteriaceae is often enumerated and presumptively identified on chromogenic media, such as chromagar(tm) orientation medium based on colony profile; however, classification is highly arbitrary, and some could not be differentiated due to similar chromogen production. here, we investigated the ability of the laser optical sensor, bardot (bacterial rapid detection usin ... | 2015 | 26503189 |
| immune-relevant and antioxidant activities of vitellogenin and yolk proteins in fish. | vitellogenin (vtg), the major egg yolk precursor protein, is traditionally thought to provide protein- and lipid-rich nutrients for developing embryos and larvae. however, the roles of vtg as well as its derived yolk proteins lipovitellin (lv) and phosvitin (pv) extend beyond nutritional functions. accumulating data have demonstrated that vtg, lv and pv participate in host innate immune defense with multifaceted functions. they can all act as multivalent pattern recognition receptors capable of ... | 2015 | 26506386 |
| direct screening of blood by pcr and pyrosequencing for a 16s rrna gene target from emergency department and intensive care unit patients being evaluated for bloodstream infection. | here we compared the results of pcr/pyrosequencing to those of culture for detecting bacteria directly from blood. dna was extracted from 1,130 blood samples from 913 patients suspected of bacteremia (enrollment criteria were physician-ordered blood culture and complete blood count [cbc]), and 102 controls (healthy blood donors). real-time pcr assays for beta-globin and universal 16s rrna gene targets were performed on all 1,232 extracts. specimens identified by universal 16s rrna gene pcr/pyros ... | 2015 | 26511737 |
| direct screening of blood by pcr and pyrosequencing for a 16s rrna gene target from emergency department and intensive care unit patients being evaluated for bloodstream infection. | here we compared the results of pcr/pyrosequencing to those of culture for detecting bacteria directly from blood. dna was extracted from 1,130 blood samples from 913 patients suspected of bacteremia (enrollment criteria were physician-ordered blood culture and complete blood count [cbc]), and 102 controls (healthy blood donors). real-time pcr assays for beta-globin and universal 16s rrna gene targets were performed on all 1,232 extracts. specimens identified by universal 16s rrna gene pcr/pyros ... | 2015 | 26511737 |
| the role of plant-microbe interactions and their exploitation for phytoremediation of air pollutants. | since air pollution has been linked to a plethora of human health problems, strategies to improve air quality are indispensable. despite the complexity in composition of air pollution, phytoremediation was shown to be effective in cleaning air. plants are known to scavenge significant amounts of air pollutants on their aboveground plant parts. leaf fall and runoff lead to transfer of (part of) the adsorbed pollutants to the soil and rhizosphere below. after uptake in the roots and leaves, plants ... | 2015 | 26516837 |
| lateral dissemination and inter-patient transmission of blakpc-3: role of a conjugative plasmid in spreading carbapenem resistance. | the objective of this study was to describe the nosocomial spread of carbapenemase-producing enterobacteria and characterize a plasmid involved in kpc dissemination. | 2016 | 26518052 |
| analysis of the structure and function of fox-4 cephamycinase. | class c β-lactamases poorly hydrolyze cephamycins (e.g., cefoxitin, cefotetan, and moxalactam). in the past 2 decades, a new family of plasmid-based ampc β-lactamases conferring resistance to cefoxitin, the fox family, has grown to include nine unique members descended from the aeromonas caviae chromosomal ampc. to understand the basis for the unique cephamycinase activity in the fox family, we determined the first x-ray crystal structures of fox-4, apo enzyme and the acyl-enzyme with its namesa ... | 2016 | 26525784 |
| cefotaxime and amoxicillin-clavulanate synergism against extended-spectrum-β-lactamase-producing escherichia coli in a murine model of urinary tract infection. | we investigated the efficacies of cefotaxime (ctx) and amoxicillin (amx)-clavulanate (cla) (amc) against extended-spectrum-β-lactamase (esbl)-producing escherichia coli in vitro and in a murine model of urinary tract infection (uti). mics, the checkerboard dilution method, and time-kill curves were used to explore the in vitro synergism between cefotaxime and amoxicillin-clavulanate against two isogenic e. coli strains-cft073-rr and its transconjugant, cft073-rr tc bla(ctx-m-15)-harboring a bla( ... | 2015 | 26525800 |
| cefotaxime and amoxicillin-clavulanate synergism against extended-spectrum-β-lactamase-producing escherichia coli in a murine model of urinary tract infection. | we investigated the efficacies of cefotaxime (ctx) and amoxicillin (amx)-clavulanate (cla) (amc) against extended-spectrum-β-lactamase (esbl)-producing escherichia coli in vitro and in a murine model of urinary tract infection (uti). mics, the checkerboard dilution method, and time-kill curves were used to explore the in vitro synergism between cefotaxime and amoxicillin-clavulanate against two isogenic e. coli strains-cft073-rr and its transconjugant, cft073-rr tc bla(ctx-m-15)-harboring a bla( ... | 2015 | 26525800 |
| the spread of bla oxa-48 and bla oxa-244 carbapenemase genes among klebsiella pneumoniae, proteus mirabilis and enterobacter spp. isolated in moscow, russia. | the spread of carbapenemase-producing enterobacteriaceae (cpe) is a great problem of healthcare worldwide. study of the spread for bla oxa-48-like genes coding epidemically significant carbapenemases among hospital pathogens is important for the regional and global epidemiology of antimicrobial resistance. | 2015 | 26526183 |
| description of a systematic pharmaceutical care approach intended to increase the appropriateness of medication use by elderly patients. | the pharmaceutical care practice represents a model of responsible pharmacist involvement in the pharmacotherapy optimization of various population groups, including the elderly, known to be at risk for drug-related problems. romanian pharmacists could use validated pharmaceutical care experiences to confirm their role as health-care professionals. this descriptive research presents the application in two real and different environments of practice of a structured pharmaceutical care approach co ... | 2014 | 26528011 |
| trends in extended spectrum beta-lactamase (esbl) producing enterobacteriaceae and esbl genes in a dutch teaching hospital, measured in 5 yearly point prevalence surveys (2010-2014). | this paper describes the trends in prevalence of esbl producing enterobacteriaceae (esbl-e) and esbl genes, measured in five consecutive yearly point prevalence surveys (pps). all patients present in the hospital and in a day-care clinic (including patients on dialysis) on the day of the survey, were screened for perianal esbl-e carriage. perianal swabs were taken and cultured using an enrichment broth and a selective agar plate. both phenotypic and genotypic methods were used to detect the prod ... | 2015 | 26528549 |
| directed evolution and resolution mechanism of 1, 3-propanediol oxidoreductase from klebsiella pneumoniae toward higher activity by error-prone pcr and bioinformatics. | 1, 3-propanediol oxidoreductase (pdor) is a key enzyme in glycerol bioconversion to 1,3-propanediol (1, 3-pd) which is a valuable chemical and one of the six new petrochemical products. we used error-prone pcr and activity screening to identify mutants of klebsiella pneumoniae (k. pneumoniae) pdor with improved activity. the activity of one of the identified mutants, pdor'-24, which includes a single mutation, a199s, was 48 u/mg, 4.9 times that of the wild-type enzyme. molecular docking was perf ... | 2015 | 26528716 |
| multicenter evaluation of the bruker maldi biotyper ca system for the identification of clinical aerobic gram-negative bacterial isolates. | the prompt and accurate identification of bacterial pathogens is fundamental to patient health and outcome. recent advances in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) have revolutionized bacterial identification in the clinical laboratory, but uniform incorporation of this technology in the u.s. market has been delayed by a lack of fda-cleared systems. in this study, we conducted a multicenter evaluation of the maldi biotyper ca (mbt-ca) system ... | 2015 | 26529504 |
| evaluation of antimicrobial therapy of blood culture positive healthcare-associated infections in children. | knowledge of the quality of antimicrobial therapy (amt) used for invasive healthcare-associated infections (hais) in paediatrics is scarce. influence of the final information about the isolated pathogen on the subsequent targeted amt was investigated in our study. | 2015 | 26539831 |
| spectrum adequacy of antibiotic regimens for secondary peritonitis: a retrospective analysis in intermediate and intensive care unit patients. | secondary peritonitis requires surgical source control and adequate antimicrobial treatment. antimicrobial regimens are usually selected according to local susceptibility data of individual pathogens against single agents, but this neglects both the polymicrobial nature of the infection and the use of combination therapy. we analysed the probability of common regimens to cover all relevant pathogens isolated in one patient ("spectrum adequacy rate", sar) in a real-life data set. | 2015 | 26541549 |
| structure, function, and assembly of adhesive organelles by uropathogenic bacteria. | bacteria assemble a wide range of adhesive proteins, termed adhesins, to mediate binding to receptors and colonization of surfaces. for pathogenic bacteria, adhesins are critical for early stages of infection, allowing the bacteria to initiate contact with host cells, colonize different tissues, and establish a foothold within the host. the adhesins expressed by a pathogen are also critical for bacterial-bacterial interactions and the formation of bacterial communities, including biofilms. the a ... | 2015 | 26542038 |
| development of a rapid and simplified protocol for direct bacterial identification from positive blood cultures by using matrix assisted laser desorption ionization time-of- flight mass spectrometry. | bloodstream infections represent serious conditions carrying a high mortality and morbidity rate. rapid identification of microorganisms and prompt institution of adequate antimicrobial therapy is of utmost importance for a successful outcome. aiming at the development of a rapid, simplified and efficient protocol, we developed and compared two in-house preparatory methods for the direct identification of bacteria from positive blood culture flasks (bd bactec fx system) by using matrix-assisted ... | 2015 | 26546000 |
| heterologous expression and functional characterization of the exogenously acquired aminoglycoside resistance methyltransferases rmtd, rmtd2, and rmtg. | the exogenously acquired 16s rrna methyltransferases rmtd, rmtd2, and rmtg were cloned and heterologously expressed in escherichia coli, and the recombinant proteins were purified to near homogeneity. each methyltransferase conferred an aminoglycoside resistance profile consistent with m(7)g1405 modification, and this activity was confirmed by in vitro 30s methylation assays. analyses of protein structure and interaction with s-adenosyl-l-methionine suggest that the molecular mechanisms of subst ... | 2015 | 26552988 |
| heterologous expression and functional characterization of the exogenously acquired aminoglycoside resistance methyltransferases rmtd, rmtd2, and rmtg. | the exogenously acquired 16s rrna methyltransferases rmtd, rmtd2, and rmtg were cloned and heterologously expressed in escherichia coli, and the recombinant proteins were purified to near homogeneity. each methyltransferase conferred an aminoglycoside resistance profile consistent with m(7)g1405 modification, and this activity was confirmed by in vitro 30s methylation assays. analyses of protein structure and interaction with s-adenosyl-l-methionine suggest that the molecular mechanisms of subst ... | 2015 | 26552988 |
| bacteriological profile of surgical site infections and their antibiogram: a study from resource constrained rural setting of uttarakhand state, india. | surgical site infections (ssi) constitute a major public health problem worldwide and are the second most frequently reported nosocomial infections. they are responsible for increasing the treatment cost, length of hospital stay and significant morbidity and mortality. | 2015 | 26557520 |
| use of next generation sequencing data to develop a qpcr method for specific detection of eu-unauthorized genetically modified bacillus subtilis overproducing riboflavin. | recently, the presence of an unauthorized genetically modified (gm) bacillus subtilis bacterium overproducing vitamin b2 in a feed additive was notified by the rapid alert system for food and feed (rasff). this has demonstrated that a contamination by a gm micro-organism (gmm) may occur in feed additives and has confronted for the first time,the enforcement laboratories with this type of rasff. as no sequence information of this gmm nor any specific detection or identification method was availab ... | 2015 | 26559764 |
| in vitro antimicrobial activity of a siderophore cephalosporin, s-649266, against enterobacteriaceae clinical isolates, including carbapenem-resistant strains. | s-649266 is a novel siderophore cephalosporin antibiotic with a catechol moiety on the 3-position side chain. two sets of clinical isolate collections were used to evaluate the antimicrobial activity of s-649266 against enterobacteriaceae. these sets included 617 global isolates collected between 2009 and 2011 and 233 β-lactamase-identified isolates, including 47 kpc-, 49 ndm-, 12 vim-, and 8 imp-producers. the mic90 values of s-649266 against the first set of escherichia coli, klebsiella pneumo ... | 2016 | 26574013 |
| secondary metabolites extracted from marine sponge associated comamonas testosteroni and citrobacter freundii as potential antimicrobials against mdr pathogens and hypothetical leads for vp40 matrix protein of ebola virus: an in vitro and in silico investigation. | the current study explores therapeutic potential of metabolites extracted from marine sponge (cliona sp.)-associated bacteria against mdr pathogens and predicts the binding prospective of probable lead molecules against vp40 target of ebola virus. the metabolite-producing bacteria were characterized by agar overlay assay and as per the protocols in bergey's manual of determinative bacteriology. the antibacterial activities of extracted metabolites were tested against clinical pathogens by well-d ... | 2016 | 26577929 |
| prevalence of extended-spectrum cephalosporin-resistant escherichia coli in a farrowing farm: st1121 clone harboring inchi2 plasmid contributes to the dissemination of bla cmy-2. | during a regular monitoring of antimicrobial resistance in a farrowing farm in southern china, 117 escherichia coli isolates were obtained from sows and piglets. compared with the isolates from piglets, the isolates from sows exhibited higher resistance rates to the tested cephalosporins. correspondingly, the total detection rate of the bla cmy-2/bla ctx-m genes in the sow isolates (34.2%) was also significantly higher than that of the piglet isolates (13.6%; p < 0.05). the bla cmy-2 gene had a ... | 2015 | 26579110 |
| comparative study of a novel biochemical assay, the rapidec carba np test, for detecting carbapenemase-producing enterobacteriaceae. | the novel biochemical test, the rapidec carba np (rcnp), was evaluated using carbapenemase- and non-carbapenemase-producing enterobacteriaceae isolates. the rcnp test was compared with the carba np test (cnp) and the modified hodge test. compared to the cnp test, the rcnp test had identical sensitivity (96%) and lower specificity (93% versus 100%). the medium used to culture the isolates significantly affected test sensitivity and specificity. the rcnp test was quicker and easier to perform than ... | 2016 | 26582833 |
| evaluation of an image analysis device (apas) for screening urine cultures. | while advancements have been made in some areas of pathology with diagnostic materials being screened using image analysis technologies, the reporting of cultures from agar plates remains a manual process. we compared the results for 2,163 urine cultures read by a reference panel of microbiologists, by the routine laboratory process, and by an automated plate reading system, apas (lbt innovations ltd., south australia). apas detected colonies with a sensitivity of 99.1% and a specificity of 99.3 ... | 2016 | 26582838 |
| group 3 innate lymphoid cells continuously require the transcription factor gata-3 after commitment. | the transcription factor gata-3 is indispensable for the development of all innate lymphoid cells (ilcs) that express the interleukin 7 receptor α-chain (il-7rα). however, the function of low gata-3 expression in committed group 3 ilcs (ilc3 cells) has not been identified. we found that gata-3 regulated the homeostasis of ilc3 cells by controlling il-7rα expression. in addition, gata-3 served a critical function in the development of the nkp46(+) ilc3 subset by regulating the balance between the ... | 2015 | 26595886 |
| group 3 innate lymphoid cells continuously require the transcription factor gata-3 after commitment. | the transcription factor gata-3 is indispensable for the development of all innate lymphoid cells (ilcs) that express the interleukin 7 receptor α-chain (il-7rα). however, the function of low gata-3 expression in committed group 3 ilcs (ilc3 cells) has not been identified. we found that gata-3 regulated the homeostasis of ilc3 cells by controlling il-7rα expression. in addition, gata-3 served a critical function in the development of the nkp46(+) ilc3 subset by regulating the balance between the ... | 2015 | 26595886 |
| investigation of yersinia pestis laboratory adaptation through a combined genomics and proteomics approach. | the bacterial pathogen yersinia pestis, the cause of plague in humans and animals, normally has a sylvatic lifestyle, cycling between fleas and mammals. in contrast, laboratory-grown y. pestis experiences a more constant environment and conditions that it would not normally encounter. the transition from the natural environment to the laboratory results in a vastly different set of selective pressures, and represents what could be considered domestication. understanding the kinds of adaptations ... | 2015 | 26599979 |
| continuous control of tracheal cuff pressure for vap prevention: a collaborative meta-analysis of individual participant data. | underinflation of tracheal cuff is a risk factor for microaspiration of contaminated secretions and subsequent ventilator-associated pneumonia (vap). the aim of this collaborative meta-analysis of individual participant data is to determine the impact of continuous control of p cuff on the incidence of vap. | 2015 | 26603289 |
| outbreak of gastroenteritis among medical students, madhya pradesh, central india. | although diarrheal diseases with known etiologies are under regular surveillance by the integrated disease surveillance project in india, only limited food-borne outbreaks were subjected to systematic epidemiological investigation. we examined one incidence of a food-borne outbreak among medical students in bhopal, india, to identifying the source and mode of transmission, and to implement appropriate preventive measures. | 2015 | 26604614 |
| detection of carbapenemase production in a collection of enterobacteriaceae with characterized resistance mechanisms from clinical and environmental origins by use of both carba np and blue-carba tests. | rapid-screening methods to confirm the presence of resistance mechanisms in multidrug-resistant bacteria are currently recommended. carba np and blue-carba tests were evaluated in carbapenemase-producing enterobacteriaceae from hospital (n = 102) and environmental (n = 57) origins for detecting the different molecular classes among them. both methods showed to be fast and cost-effective, with high sensitivity (98% to 100%) and specificity (100%), and may be easily introduced in the routine labor ... | 2016 | 26607979 |
| use of an enrichment broth improves detection of extended-spectrum-beta-lactamase-producing enterobacteriaceae in clinical stool samples. | this study evaluated the impact of preenrichment on the detection of extended-spectrum beta-lactamase-producing enterobacteriaceae (esbl-e) in clinical stool samples. esbl-e were detected in 41 of 343 patients (12.0%). as 31.7% of the esbl-e carriers were identified by preenrichment, only this additional diagnostic step significantly improved the detection of esbl-e. | 2016 | 26607984 |
| epidemiologic and genotypic review of carbapenemase-producing organisms in british columbia, canada, between 2008 and 2014. | carbapenemase-producing organisms (cpos) are a serious emerging problem for health care facilities worldwide. owing to their resistance to most antimicrobial therapies, cpos are difficult to treat and pose a challenge for infection prevention and control. since 2010, lab-based surveillance for cpos and pcr-based testing were implemented in british columbia (bc), canada. a review of cpos in bc from 2008 to march 2014 was done to characterize the resistance mechanisms and possible clonal strain tr ... | 2016 | 26607987 |
| tuning the phosphoryl donor specificity of dihydroxyacetone kinase from atp to inorganic polyphosphate. an insight from computational studies. | dihydroxyacetone (dha) kinase from citrobacter freundii provides an easy entry for the preparation of dha phosphate; a very important c3 building block in nature. to modify the phosphoryl donor specificity of this enzyme from atp to inorganic polyphosphate (poly-p); a directed evolution program has been initiated. in the first cycle of evolution, the native enzyme was subjected to one round of error-prone pcr (ep-pcr) followed directly (without selection) by a round of dna shuffling. although th ... | 2015 | 26610480 |
| antibacterial suture vs silk for the surgical removal of impacted lower third molars. a randomized clinical study. | the aim of this study was to evaluate the clinical and microbiological impact of an antibacterial suture (monocryl® plus) in the surgical removal of i3m. | 2015 | 26615503 |
| antibacterial suture vs silk for the surgical removal of impacted lower third molars. a randomized clinical study. | the aim of this study was to evaluate the clinical and microbiological impact of an antibacterial suture (monocryl® plus) in the surgical removal of i3m. | 2015 | 26615503 |
| prevalence of multidrug resistant uropathogenic bacteria in pediatric patients of a tertiary care hospital in eastern india. | today, because systemic infections such as urinary tract infection (uti) affect even pediatric patients, antibiotic resistant bacteria have become a constant clinical challenge. in the present study, a total of 1054 urine samples were collected from pediatric patients over 18 months. from these samples, 510 isolates of pathogenic bacteria were collected using hicrome uti agar. antibiotic sensitivity tests of isolates were performed using the kirby-bauer method. two gram-positive bacteria (entero ... | 2017 | 26617250 |
| a rare case of ecthyma gangrenosum caused by proteus vulgaris and candida albicans in a patient with castleman disease. | 2015 | 26618137 | |
| antibiogram and heavy metal tolerance of bullfrog bacteria in malaysia. | bacterial isolates from 30 farmed bullfrogs (lithobates catesbeianus) weighing 500-600 g at johore, malaysia with external clinical signs of ulcer, red leg and torticollis were tested for their antibiograms and heavy metal tolerance patterns. a total of 17 bacterial species with 77 strains were successfully isolated and assigned to 21 antibiotics and 4 types of heavy metal (hg(2+), cr(6+), cd(2+), cu(2+)). results revealed that bacteria were resistant against lincomycin (92%), oleandomycin (72.7 ... | 2011 | 26623279 |
| screening of nursing home residents for colonization with carbapenem-resistant enterobacteriaceae admitted to acute care hospitals: incidence and risk factors. | there are increasing reports of multidrug-resistant gram-negative bacilli in nursing homes and acute care hospitals. | 2016 | 26631643 |
| associations between single nucleotide polymorphisms of high mobility group box 1 protein and clinical outcomes in korean sepsis patients. | high mobility group box 1 (hmgb1) plays a central role in the pathogenesis of sepsis and multiple organ dysfunction syndromes. we investigated the associations of a single nucleotide polymorphism (snp; rs1045411) in hmgb1 with various clinical parameters, severity, and prognosis in patients with sepsis, severe sepsis, or septic shock. | 2015 | 26632390 |
| associations between single nucleotide polymorphisms of high mobility group box 1 protein and clinical outcomes in korean sepsis patients. | high mobility group box 1 (hmgb1) plays a central role in the pathogenesis of sepsis and multiple organ dysfunction syndromes. we investigated the associations of a single nucleotide polymorphism (snp; rs1045411) in hmgb1 with various clinical parameters, severity, and prognosis in patients with sepsis, severe sepsis, or septic shock. | 2015 | 26632390 |
| evaluation of presurgical skin preparation agents in african clawed frogs (xenopus laevis). | despite the routine collection of oocytes from african clawed frogs (xenopus laevis) for use in research, few studies have evaluated methods for preparing their skin for surgery. we evaluated 3 skin preparatory agents by examining their antibacterial efficacy and the gross and microscopic appearance of xenopus skin after exposure. frogs (n = 14) were sedated and treated (contact time, 10 min) with 0.9% sterile nacl on one-half of the ventrum and with 0.5% povidone-iodine or 0.75% chlorhexidine o ... | 2015 | 26632790 |
| mass spectrometry-based method of detecting and distinguishing type 1 and type 2 shiga-like toxins in human serum. | shiga-like toxins (verotoxins) are responsible for the virulence associated with a variety of foodborne bacterial pathogens. direct detection of toxins requires a specific and sensitive technique. in this study, we describe a mass spectrometry-based method of analyzing the tryptic decapeptides derived from the non-toxic b subunits. a gene encoding a single protein that yields a set of relevant peptides upon digestion with trypsin was designed. the (15)n-labeled protein was prepared by growing th ... | 2015 | 26633510 |
| community-acquired pneumonia and empyema caused by citrobacter koseri in an immunocompetent patient. | citrobacter species, belonging to the family enterobacteriaceae, are environmental organisms commonly found in soil, water, and the intestinal tracts of animals and humans. citrobacter koseri is known to be an uncommon but serious cause of both sporadic and epidemic septicemia and meningitis in neonates and young infants. most cases reported have occurred in immunocompromised hosts. the infections caused by citrobacter are difficult to treat with usual broad spectrum antibiotics owing to rapid g ... | 2015 | 26634165 |
| evaluation of the byg carba test, a new electrochemical assay for rapid laboratory detection of carbapenemase-producing enterobacteriaceae. | accurate detection of carbapenemase-producing enterobacteriaceae (cpe) constitutes a major laboratory diagnostic challenge. we evaluated an electrochemical technique (the byg carba test) which allows detection of cpe in less than 35 min. the byg carba test was first validated in triplicate against 57 collection isolates with previously characterized β-lactam resistance mechanisms (oxa-48, n = 12; kpc, n = 8; ndm, n = 8; vim, n = 8; imp, n = 3; gim, n = 1; ges-6, n = 1; no carbapenemase, n = 16) ... | 2016 | 26637378 |
| complete sequence of blakpc-2-harboring plasmid with a mosaic of incn1- and incn3-type plasmids in a klebsiella pneumoniae isolate from south korea. | 2016 | 26643324 | |
| multiyear, multinational survey of the incidence and global distribution of metallo-β-lactamase-producing enterobacteriaceae and pseudomonas aeruginosa. | metallo-β-lactamases (mbls) hydrolyze all classes of β-lactams except monobactams and are not inhibited by classic serine β-lactamase inhibitors. gram-negative pathogens isolated from patient infections were collected from 202 medical centers in 40 countries as part of a global surveillance study from 2012 to 2014. carbapenem-nonsusceptible enterobacteriaceae and pseudomonas aeruginosa were characterized for bla genes encoding vim, imp, ndm, spm, and gim variants using pcr and sequencing. a tota ... | 2016 | 26643349 |
| structural and functional analyses reveal insights into the molecular properties of the escherichia coli z ring stabilizing protein, zapc. | in escherichia coli cell division is driven by the tubulin-like gtpase, ftsz, which forms the cytokinetic z-ring. the z-ring serves as a dynamic platform for the assembly of the multiprotein divisome, which catalyzes membrane cleavage to create equal daughter cells. several proteins effect ftsz assembly, thereby providing spatiotemporal control over cell division. one important class of ftsz interacting/regulatory proteins is the z-ring-associated proteins, zaps, which typically modulate z-ring ... | 2015 | 26655719 |
| structural and functional analyses reveal insights into the molecular properties of the escherichia coli z ring stabilizing protein, zapc. | in escherichia coli cell division is driven by the tubulin-like gtpase, ftsz, which forms the cytokinetic z-ring. the z-ring serves as a dynamic platform for the assembly of the multiprotein divisome, which catalyzes membrane cleavage to create equal daughter cells. several proteins effect ftsz assembly, thereby providing spatiotemporal control over cell division. one important class of ftsz interacting/regulatory proteins is the z-ring-associated proteins, zaps, which typically modulate z-ring ... | 2015 | 26655719 |
| mesaconase/fumarase fumd in escherichia coli o157:h7 and promiscuity of escherichia coli class i fumarases fuma and fumb. | mesaconase catalyzes the hydration of mesaconate (methylfumarate) to (s)-citramalate. the enzyme participates in the methylaspartate pathway of glutamate fermentation as well as in the metabolism of various c5-dicarboxylic acids such as mesaconate or l-threo-β-methylmalate. we have recently shown that burkholderia xenovorans uses a promiscuous class i fumarase to catalyze this reaction in the course of mesaconate utilization. here we show that classical escherichia coli class i fumarases a and b ... | 2015 | 26658641 |
| correlation of β-lactamase production and colistin resistance among enterobacteriaceae isolates from a global surveillance program. | the increasing use of carbapenems for treating multidrug-resistant (mdr) gram-negative bacterial infections has contributed to the global dissemination of carbapenem-resistant enterobacteriaceae (cre). serine and metallo-β-lactamases (mbls) that hydrolyze carbapenems have become prevalent and endemic in some countries, necessitating the use of older classes of agents, such as colistin. a total of 19,719 isolates of enterobacteriaceae (excluding proteeae and serratia spp., which have innate resis ... | 2016 | 26666920 |
| emergence of oxa-162 carbapenemase- and dha-1 ampc cephalosporinase-producing sequence type 11 klebsiella pneumoniae causing community-onset infection in greece. | oxa-48-like carbapenemases have only recently emerged in europe. oxa-162 is a rare oxa-48 variant usually coexpressed with extended-spectrum β-lactamases. here, we report the identification of the first oxa-162 carbapenemase-producing klebsiella pneumoniae isolates, which coexpressed an ampc cephalosporinase (dha-1), retrieved from a patient in greece. they belonged to a single sequence type (st11) and caused the first documented community-onset urinary tract infections attributable to an oxa-48 ... | 2016 | 26666930 |
| β-lactamase characterization of gram-negative pathogens recovered from patients enrolled in the phase 2 trials for ceftazidime-avibactam: clinical efficacies analyzed against subsets of molecularly characterized isolates. | the correlation of the clinical efficacies of ceftazidime-avibactam and comparators (carbapenems) was evaluated against baseline gram-negative isolates having characterized β-lactam resistance mechanisms from complicated urinary tract infection (cuti) and complicated intra-abdominal infection (ciai) phase 2 trials. enterobacteriaceae displaying ceftriaxone and/or ceftazidime mics of ≥ 2 μg/ml (69 isolates) and nonfermentative gram-negative bacilli (nf-gnb [three isolates]) with ceftazidime mics ... | 2016 | 26666936 |
| risk factors and outcomes for intestinal carriage of ampc-hyperproducing enterobacteriaceae in intensive care unit patients. | in a cohort of 1,209 intensive care unit (icu) patients, the prevalence of intestinal colonization with high-level expressed ampc cephalosporinase-producing enterobacteriaceae (hlac-pe) rose steadily from 2% at admission to 30% in patients with lengths of stay (los) exceeding 4 weeks. in multivariate analysis, los was the main predictor of carriage acquisition after adjustment on antimicrobial exposure. hlac-pe infection occurred in 15% of carriers. carriage and infection were associated with a ... | 2016 | 26666938 |
| 2d/3d/4d contrast-enhanced voiding urosonography in the diagnosis and monitoring of treatment of vesicoureteral reflux in children - can it replace voiding cystourethrography? | vesicoureteral reflux appears in 20-50% of pediatric patients with recurrent urinary tract infections. the most common method of diagnosing this disease is voiding cystourethrography. however, contemporary pediatric radiology does not favor this method due to exposure to x-radiation. | 2013 | 26674600 |
| [antimicrobial resistance monitoring of gram-negative bacilli isolated from 15 teaching hospitals in 2014 in china]. | to investigate the current situation of antimicrobial resistance of nosocomial gram-negative bacilli in 2014 in china. | 2015 | 26675021 |
| rapid detection of oxa-48-producing enterobacteriaceae by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | a rapid and sensitive (100%) matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) assay was developed to detect oxa-48-type producers, using 161 previously characterized clinical isolates. ertapenem was monitored to detect carbapenem resistance, and temocillin was included in the assay as a marker for oxa-48-producers. structural analysis of temocillin is described. data are obtained within 60 min. | 2016 | 26677247 |