Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| antibiotic resistance among campylobacter jejuni/coli strains acquired in sweden and abroad: a longitudinal study. | antibiotic resistance was compared in 844 campylobacter jejuni/coli strains acquired outside sweden and 575 acquired in sweden during 1990-2002. there was a clear gradual increase in ciprofloxacin and tetracycline resistance among c. jejuni/coli strains acquired outside sweden during the 13 y period. this trend was not seen for erythromycin or in domestically acquired strains for any of the 3 antibiotics tested. | 2003 | 14514147 |
| disinfectant susceptibility testing of avian and swine campylobacter isolates by a filtration method. | the susceptibility testing of disinfectants against campylobacter jejuni and campylobacter coli strains from broilers and pigs was investigated. the filtration method european standard en 1040 was adapted to campylobacter cultures and validated with reference strains. two disinfectants were tested: 1% benzalkonium chloride active matter, as quaternary ammonium compound, and 0.63% sodium hypochlorite as chlorine-releasing agent. both disinfectants were effective against the 34 campylobacter strai ... | 2003 | 14516706 |
| simultaneous detection of mutations associated with resistance to macrolides and quinolones in campylobacter jejuni and c. coli using a pcr-line probe assay. | quinolone and macrolide resistance of campylobacter jejuni and campylobacter coli mainly depend on a mutation in gyra and in 23s rdna, respectively. in order to detect quinolone and/or macrolide resistant c. jejuni and c. coli strains, a macrolide and quinolone line probe assay (mq-lipa) was developed and 42 c. jejuni and c. coli strains were tested to evaluate the efficiency of the assay. profiles of the mutations in 23s rdna and in gyra characterized by mq-lipa agreed with resistance to macrol ... | 2003 | 14522100 |
| application of real-time pcr for quantitative detection of campylobacter jejuni in poultry, milk and environmental water. | campylobacter jejuni is a leading human food-borne pathogen. the rapid and sensitive detection of c. jejuni is necessary for the maintenance of a safe food/water supply. in this article, we present a real-time polymerase chain reaction (pcr) assay for quantitative detection of c. jejuni in naturally contaminated poultry, milk and environmental samples without an enrichment step. the whole assay can be completed in 60 min with a detection limit of approximately 1 cfu. the standard curve correlati ... | 2003 | 14522462 |
| ribotyping characterisation of campylobacter isolates randomly collected from different sources in italy. | in this study the potential for using the automated psti ribotyping as a primary library typing method to survey campylobacter and for identification of two thermophilic campylobacter jejuni and campylobacter coli species was evaluated. a total of 158 isolates randomly collected in italy from different sources were analyzed. a large percentage of chicken (28%), turkey (27%) and turkey meat (25%) isolates shared their ribotyping profiles (ribotypes) with those of humans, whereas the swine isolate ... | 2003 | 14522511 |
| comparison of the bax system with a multiplex pcr method for simultaneous detection and identification of campylobacter jejuni and campylobacter coli in environmental samples. | the campylobacter detection is performed by conventional culture methods and the identification of campylobacter jejuni and campylobacter coli is principally based on the hippurate hydrolysis test. the two major drawbacks of this biochemical test for species identification include the inconsistency of the results and the presence of atypical strains, which can lead to the misidentification of an isolate. as an alternative, multiplex polymerase chain reaction (mpcr) protocols for the simultaneous ... | 2003 | 14527799 |
| a 6 x 6 drop plate method for simultaneous colony counting and mpn enumeration of campylobacter jejuni, listeria monocytogenes, and escherichia coli. | a protocol was developed using 96-well plates and multichannel pipettes for serial dilutions, followed by drop plating on agar in a 6 x 6 format. this protocol permits simultaneous plating of six dilutions which greatly decreases the number of plates utilized thereby saving incubator space for organisms such as campylobacter which require unique environmental conditions. | 2003 | 14529971 |
| using quantitative real-time pcr to determine the hygienic status of cattle manure. | we developed a suitable system of dna extraction and real-time quantitative polymerase chain reaction (qpcr) for the specific and sensitive quantification of pathogens and other relevant (indicator) organisms in recalcitrant material such as cattle manure. pcr inhibition by coextraction of humic compounds was minimized in this system, resulting in detection sensitivity of one target dna copy per reaction well. data from qpcr analysis for escherichia coli agreed with cultivation based results, bu ... | 2003 | 14531427 |
| a model to estimate the optimal sample size for microbiological surveys. | estimating optimal sample size for microbiological surveys is a challenge for laboratory managers. when insufficient sampling is conducted, biased inferences are likely; however, when excessive sampling is conducted valuable laboratory resources are wasted. this report presents a statistical model for the estimation of the sample size appropriate for the accurate identification of the bacterial subtypes of interest in a specimen. this applied model for microbiology laboratory use is based on a b ... | 2003 | 14532078 |
| application of host-specific bacteriophages to the surface of chicken skin leads to a reduction in recovery of campylobacter jejuni. | retail poultry products are widely purported as the major infection vehicle for human campylobacteriosis. numerous intervention strategies have sought to reduce campylobacter contamination on broiler carcasses in the abattoir. this study reports the efficacy of bacteriophage in reducing the number of recoverable campylobacter jejuni cells on artificially contaminated chicken skin. | 2003 | 14532096 |
| comparison of campylobacter isolates from poultry and humans: association between in vitro virulence properties, biotypes, and pulsed-field gel electrophoresis clusters. | the in vitro virulence properties of 197 temporally and geographically related campylobacter isolates from chicken broilers and humans were compared. comparisons of the virulence properties associated with genotypes and biotypes were made. all isolates adhered to, and 63% invaded, int-407 cells, whereas 13% were cytotoxic for cho cells. cho cell-cytotoxic extracts were also cytotoxic for int-407 cells, but the sensitivity for vero cells was variable. the proportion of isolates demonstrating a hi ... | 2003 | 14532099 |
| molecular subtyping of vibrio cholerae o1 and o139 by pulsed-field gel electrophoresis in hong kong: correlation with epidemiological events from 1994 to 2002. | two hundred twenty isolates of vibrio cholerae o1 and o139 collected from 1994 to 2002 in hong kong were analyzed by pulsed-field gel electrophoresis (pfge). chromosomal dnas from all v. cholerae isolates in agarose plugs were digested with the restriction enzyme noti, resulting in 20 to 27 bands. sixty distinctive pfge patterns in the range of 10 to 300 kb were noted among 213 isolates typeable by pfge. by comparing the common pfge patterns obtained from four well-defined outbreaks of v. choler ... | 2003 | 14532174 |
| evaluation of protocol using gene capture and pcr for detection of helicobacter pylori dna in feces. | the route of transmission of helicobacter pylori, which is usually acquired in childhood and is one of the most common bacterial infections in humans, remains undetermined. mapping the distribution of h. pylori genotypes within families could help to determine the routes of transmission and risk factors. here we describe a noninvasive method for obtaining h. pylori dna isolates from the feces of children. children presenting with gastrointestinal symptoms at the royal hospital for sick children ... | 2003 | 14532187 |
| diagnosis of helicobacter pylori infection in adults with intellectual disability. | helicobacter pylori infection is common among adults with intellectual disability. the acceptabilities and accuracies of different diagnostic tests in this population are unknown. we aimed to determine (i) patient acceptability and (ii) performance characteristics of serology, fecal-antigen, and urea breath tests among adults with intellectual disability. one hundred sixty-eight such adults underwent h. pylori testing with serology and fecal-antigen tests, and a portion underwent treatment. one ... | 2003 | 14532206 |
| utility of multilocus sequence typing as an epidemiological tool for investigation of outbreaks of gastroenteritis caused by campylobacter jejuni. | multilocus sequence typing (mlst) has been proven useful for the study of the global population structure of campylobacter jejuni; however, its usefulness for the investigation of outbreaks of disease caused by c. jejuni has not been proven. in this study, mlst plus sequencing of the flaa short variable region (svr) were applied to 47 isolates from 12 outbreaks of c. jejuni infection whose relatedness has been determined previously, and the results were compared to those of serotyping and pulsed ... | 2003 | 14532212 |
| temporal and geographical distribution and overlap of penner heat-stable serotypes and pulsed-field gel electrophoresis genotypes of campylobacter jejuni isolates collected from humans and chickens in finland during a seasonal peak. | the association of penner heat-stable serotypes and pulsed-field gel electrophoresis genotypes of 208 human and 30 chicken campylobacter jejuni isolates was studied. overall, 46% of the human strains had overlapping sero- and genotype combinations with chicken strains. the percentage was reduced to 31% for strains that were considered temporally related. this suggests common environmental sources. | 2003 | 14532246 |
| high prevalence of atypical enteropathogenic escherichia coli (epec) in norwegian children with diarrhoea. | the aim of the present study was to investigate the relative contribution of enteropathogenic escherichia coli (epec) as a cause of infectious diarrhoea in norwegian children. data from faecal specimens from children <2 years old with diarrhoea during the year 2001 were analysed. e. coli isolates with the attaching and effacing genotype (eae+) were examined for the presence of the bundle-forming pilus (bfpa) and shiga toxin genes by pcr, and for genetic relatedness by pfge. during the 1-year per ... | 2003 | 14532347 |
| molecular mimicry of host structures by lipooligosaccharides of neisseria meningitidis: characterization of sialylated and nonsialylated lacto-n-neotetraose (galbeta1-4glcnacbeta1-3galbeta1-4glc) structures in lipooligosaccharides using monoclonal antibodies and specific lectins. | neisseria meningitidis lipooligosaccharides (loss) are classified into 12 immunotypes. most loss are heterogeneous in having a few components by sds-page analysis that differ antigenically and chemically. we have utilized a monoclonal antibody that recognizes lacto-n-neotetraose (lnnt) and the lectin, maackia amurensis leukoagglutinin (mal), which is specific for neunacalpha2-3galbeta1-4glcnac trisacchride sequence to characterize the 12 n. meningitidis loss. using the combination of elisa, sds- ... | 2001 | 14533820 |
| gammadelta t cell non-responsiveness in campylobacter jejuni-associated guillain-barré syndrome patients. | to seek evidence for a role of molecular mimicry in the induction of guillain-barré syndrome (gbs), the authors studied campylobacter jejuni-reactive t lymphocytes in patients with gbs. in contrast to controls, gammadelta t cells of patients with gbs with antecedent c jejuni infections failed to respond to c jejuni. supplementing cell cultures with the cytokines interleukin-2 or interleukin-15 resulted in restoration of the gammadelta t cell proliferative response. gammadelta t cell non-responsi ... | 2003 | 14557578 |
| antimicrobial resistance in campylobacter from broilers: association with production type and antimicrobial use. | the isolation and antimicrobial resistance of campylobacter jejuni and campylobacter coli strains from broilers arriving in french slaughterhouses, were analysed according to production types (i.e. standard, export or free-range) and antimicrobial (i.e. coccidiostats, growth promoters or therapeutic agents) administration in flocks. prevalence was 56.6% in standard, 51.3% in export and 80.0% in free-range broilers. three hundred and ninety-three strains were identified. two-thirds of the strains ... | 2003 | 14559174 |
| functionality of system components: conservation of protein function in protein feature space. | many protein features useful for prediction of protein function can be predicted from sequence, including posttranslational modifications, subcellular localization, and physical/chemical properties. we show here that such protein features are more conserved among orthologs than paralogs, indicating they are crucial for protein function and thus subject to selective pressure. this means that a function prediction method based on sequence-derived features may be able to discriminate between protei ... | 2003 | 14559779 |
| failure of viable nonculturable campylobacter jejuni to colonize the cecum of newly hatched leghorn chicks. | campylobacter jejuni cells entered the viable but nonculturable (vbnc) state upon suspension in sterile water. cell viability was determined with tetrazolium violet. vbnc cells suspended in water for 7, 10, or 14 days were given, by gavage, to day-of-hatch leghorn chickens. the ceca of control and challenged birds were examined for the presence of campylobacteria by conventional microbiological methods at 1 wk and 2 wk after challenge inoculation and by polymerase chain reaction methods at 1 wk ... | 2003 | 14562908 |
| development of a selective broth medium for the detection of injured campylobacter jejuni by capacitance monitoring. | the purpose of these studies was to develop a conductimetric method for the rapid detection of campylobacter jejuni. numerous basal medium components were analyzed to develop a growth-enhancing broth medium for detection of freeze-injured campylobacter cells using a conductimetric system. the final medium was composed of a modified campy-line agar from which the agar and triphenyltetrazolium chloride were removed and the amino acid, l-arginine was added. pure isolates of c. jejuni. (frozen and t ... | 2003 | 14572208 |
| antibacterial activities of phenolic benzaldehydes and benzoic acids against campylobacter jejuni, escherichia coli, listeria monocytogenes, and salmonella enterica. | we evaluated the bactericidal activities of 35 benzaldehydes, 34 benzoic acids, and 1 benzoic acid methyl ester against campylobacter jejuni, escherichia coli o157:h7, listeria monocytogenes, and salmonella enterica when these compounds were substituted on the benzene ring with 0, 1, 2, or 3 hydroxy (oh) and/or methoxy (och3) groups in a ph 7.0 buffer. dose-response plots were used to determine the percentage of the sample that induced a 50% decrease in cfu after 60 min (ba50). of the 70 compoun ... | 2003 | 14572218 |
| consumer food-handling behaviors associated with prevention of 13 foodborne illnesses. | to be effective in reducing the incidence of foodborne illness, consumers and food safety educators need information about behaviors that will decrease exposure to foodborne pathogens. a four-round delphi technique was used to survey nationally recognized experts in food microbiology, epidemiology, food safety education, and food safety policy with the aim of identifying and ranking food-handling and consumption behaviors associated with 13 major foodborne pathogens. the food safety experts rank ... | 2003 | 14572229 |
| comparison of monte carlo and fuzzy math simulation methods for quantitative microbial risk assessment. | the objective of food safety risk assessment is to quantify levels of risk for consumers as well as to design improved processing, distribution, and preparation systems that reduce exposure to acceptable limits. monte carlo simulation tools have been used to deal with the inherent variability in food systems, but these tools require substantial data for estimates of probability distributions. the objective of this study was to evaluate the use of fuzzy values to represent uncertainty. fuzzy math ... | 2003 | 14572230 |
| identification of a gene that affects the efficiency of host cell infection by legionella pneumophila in a temperature-dependent fashion. | the ability to infect host cells is critical for the survival and replication of intracellular pathogens in humans. we previously found that many genes involved in the ability of legionella pneumophila to infect macrophages are not expressed efficiently under standard laboratory growth conditions. we have developed an approach using expression of l. pneumophila genes from an exogenous constitutive promoter on a low-copy-number vector that allows identification of genes involved in host cell infe ... | 2003 | 14573644 |
| a cdta-cdtc complex can block killing of hela cells by haemophilus ducreyi cytolethal distending toxin. | the cytolethal distending toxin (cdt) of haemophilus ducreyi is comprised of the cdta, cdtb, and cdtc proteins, with the cdtb protein having demonstrated enzymatic (i.e., dnase) activity. using a single recombinant escherichia coli strain with two plasmids individually containing the h. ducreyi cdta and cdtc genes, we purified a noncovalent cdta-cdtc complex. incubation of this cdta-cdtc complex with hela cells blocked killing of these cells by cdt holotoxin. furthermore, the addition of purifie ... | 2003 | 14573688 |
| transcription profiles of the bacterium mycoplasma pneumoniae grown at different temperatures. | applying microarray technology, we have investigated the transcriptome of the small bacterium mycoplasma pneumoniae grown at three different temperature conditions: 32, 37 and 32 degrees c followed by a heat shock for 15 min at 43 degrees c, before isolating the rna. from 688 proposed open-reading frames, 676 were investigated and 564 were found to be expressed (p < 0.001; 606 with p < 0.01) and at least 33 (p < 0.001; 77 at p < 0.01) regulated. by quantitative real-time pcr of selected mrna spe ... | 2003 | 14576319 |
| planktonic or biofilm growth affects survival, hydrophobicity and protein expression patterns of a pathogenic campylobacter jejuni strain. | the effect of planktonic or biofilm modes of growth on survival, hydrophobicity and cellular protein expression patterns of a pathogenic campylobacter jejuni strain were determined. this was achieved by growing the strain in brain heart infusion broth (with 1% yeast extract), or attached to glass beads in the same medium, at 37 degrees c for 48 h under microaerophilic conditions. cells from the broth or the bead surfaces were stored at different temperatures (4, 10, 25 and 37 degrees c) for 28 d ... | 2003 | 14580968 |
| extensive repetitive dna facilitates prokaryotic genome plasticity. | prokaryotic genomes are substantially diverse, even when from closely related species, with the resulting phenotypic diversity representing a repertoire of adaptations to specific constraints. within the microbial population, genome content may not be fixed, as changing selective forces favor particular phenotypes; however, organisms well adapted to particular niches may have evolved mechanisms to facilitate such plasticity. the highly diverse helicobacter pylori is a model for studying genome p ... | 2003 | 14593200 |
| flagellin glycosylation island in pseudomonas syringae pv. glycinea and its role in host specificity. | the deduced amino acid sequences of the flagellins of pseudomonas syringae pv. tabaci and p. syringae pv. glycinea are identical; however, their abilities to induce a hypersensitive reaction are clearly different. the reason for the difference seems to depend on the posttranslational modification of the flagellins. to investigate the role of this posttranslational modification in the interactions between plants and bacterial pathogens, we isolated genes that are potentially involved in the postt ... | 2003 | 14594840 |
| distribution of campylobacter jejuni penner serotypes in broiler flocks 1998-2000 in a small danish community with special reference to serotype 4-complex. | during the period january 1998-december 2001, all danish broiler flocks were monitored bacteriologically for thermophilic campylobacters and isolates were stored at -80 degrees c. six neighbouring broiler farms in a small community were selected for detailed examination of all campylobacter jejuni isolated (n = 180) from these farms during 1998-2000 using penner serotyping and pulsed-field gel electrophoresis (pfge). the area and the farms were selected according to their prevalence of campyloba ... | 2003 | 14596533 |
| comparative genome analysis of campylobacter jejuni using whole genome dna microarrays. | whole genome dna microarrays were constructed and used to investigate genomic diversity in 18 campylobacter jejuni strains from diverse sources. new algorithms were developed that dynamically determine the boundary between the conserved and variable genes. seven hypervariable plasticity regions (pr) were identified in the genome (pr1 to pr7) containing 136 genes (50%) of the variable gene pool. when comparisons were made with the sequenced strain nctc11168, the number of absent or divergent gene ... | 2003 | 14596944 |
| interspecies communication in bacteria. | until recently, bacteria were considered to live rather asocial, reclusive lives. new research shows that, in fact, bacteria have elaborate chemical signaling systems that enable them to communicate within and between species. one signal, termed ai-2, appears to be universal and facilitates interspecies communication. many processes, including virulence factor production, biofilm formation, and motility, are controlled by ai-2. strategies that interfere with communication in bacteria are being e ... | 2003 | 14597753 |
| multilocus sequence typing for comparison of veterinary and human isolates of campylobacter jejuni. | multilocus sequence typing (mlst) has been applied to 266 campylobacter jejuni isolates, mainly from veterinary sources, including cattle, sheep, poultry, pigs, pets, and the environment, as well as isolates from human cases of campylobacteriosis. the populations of veterinary and human isolates overlap, suggesting that most veterinary sources should be considered reservoirs of pathogenic campylobacters. there were some associations between source and sequence type complex, indicating that host ... | 2003 | 14602588 |
| induction and resuscitation of viable but nonculturable salmonella enterica serovar typhimurium dt104. | salmonella enterica serovar typhimurium dt104 11601 was tested for its ability to maintain viability in minimal, chemically defined solutions. periodic monitoring of growth and survival in microcosms of different ion concentrations, maintained at various temperatures, showed a gradual decline in culturable organisms ( approximately 235 days) at 5 degrees c. organisms maintained at a higher temperature (21 degrees c) showed continuous, equivalent cfu per milliliter ( approximately 10(6)) up to 40 ... | 2003 | 14602627 |
| web-based phylogenetic assignment tool for analysis of terminal restriction fragment length polymorphism profiles of microbial communities. | culture-independent dna fingerprints are commonly used to assess the diversity of a microbial community. however, relating species composition to community profiles produced by community fingerprint methods is not straightforward. terminal restriction fragment length polymorphism (t-rflp) is a community fingerprint method in which phylogenetic assignments may be inferred from the terminal restriction fragment (t-rf) sizes through the use of web-based resources that predict t-rf sizes for known b ... | 2003 | 14602639 |
| duplex real-time sybr green pcr assays for detection of 17 species of food- or waterborne pathogens in stools. | a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using the qiaamp dna stool mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. the protocol used the same lc-pcr with 20 pairs of specific primers. the products formed were identified based on a melting point temperature (t(m)) curve analysis. the 17 species of food- or waterborne pathogens examined were enteroinvasive escherichi ... | 2003 | 14605150 |
| enteric campylobacter: purging its secrets? | campylobacterial infections are the most common cause of bacterial enterocolitis in humans. among children, especially in developing countries, campylobacter infections can cause severe life-threatening diarrheal disease. although usually associated with a benign outcome in the developed world, the burden of illness posed by campylobacter infections is enormous, and serious neurologic sequelae also can occur. for a variety of reasons our understanding of the molecular and cellular pathogenesis o ... | 2004 | 14605259 |
| the outs and ins of bacterial type iv secretion substrates. | bacteria use type iv secretion systems (t4ss) to translocate macromolecular substrates destined for bacterial, plant or human target cells. the t4ss are medically important, contributing to virulence-gene spread, genome plasticity and the alteration of host cellular processes during infection. the t4ss are ancestrally related to bacterial conjugation machines, but present-day functions include (i) conjugal transfer of dna by cell-to-cell contact, (ii) translocation of effector molecules to eukar ... | 2003 | 14607070 |
| characterization of waterborne outbreak-associated campylobacter jejuni, walkerton, ontario. | 2003 | 14609457 | |
| purification and properties of the formate dehydrogenase and characterization of the fdha gene of sulfurospirillum multivorans. | the soluble periplasmic subunit of the formate dehydrogenase fdha of the tetrachloroethene-reducing anaerobe sulfurospirillum multivorans was purified to apparent homogeneity and the gene ( fdha) was identified and sequenced. the purified enzyme catalyzed the oxidation of formate with oxidized methyl viologen as electron acceptor at a specific activity of 1683 nkat/mg protein. the apparent molecular mass of the native enzyme was determined by gel filtration to be about 100 kda, which was confirm ... | 2003 | 14610638 |
| a golden age for microbial genomics. | 2003 | 14611656 | |
| multidrug resistance in campylobacter jejuni strains collected from finnish patients during 1995-2000. | the resistance of campylobacter jejuni to fluoroquinolones is increasing globally. this study was performed to delineate those antimicrobial agents that are effective in vitro against ciprofloxacin-resistant c. jejuni isolates and potentially suitable for the treatment of severe disease when fluoroquinolone resistance or multidrug resistance is known or suspected. | 2003 | 14613954 |
| coevolution of an aminoacyl-trna synthetase with its trna substrates. | glutamyl-trna synthetases (glurss) occur in two types, the discriminating and the nondiscriminating enzymes. they differ in their choice of substrates and use either trnaglu or both trnaglu and trnagln. although most organisms encode only one glurs, a number of bacteria encode two different glurs proteins; yet, the trna specificity of these enzymes and the reason for such gene duplications are unknown. a database search revealed duplicated glurs genes in >20 bacterial species, suggesting that th ... | 2003 | 14615592 |
| campylobacter jejuni: unusual cause of cholecystitis with lithiasis. case report and literature review. | a 51-year-old man presented with acute cholecystitis and the routine intraoperative culture of the bile grew campylobacter jejuni. the patient was cured by laparoscopic cholecystectomy without specific antimicrobial treatment. cholecystitis owing to campylobacter spp. could be missed because a culture for campylobacter is not routinely requested nor is it cost effective to look for it in bile or gallbladder specimens. moreover, the fastidious nature of these bacteria dictates against their recov ... | 2003 | 14616689 |
| evaluation of the prospect microplate assay for detection of campylobacter: a routine laboratory perspective. | to evaluate the use of the new enzyme-linked immunosorbent assay, the prospect campylobacter microplate assay (alexon-trend, minneapolis, mn, usa), which allows 2-h detection of both campylobacter jejuni and campylobacter coli antigen directly in stool specimens. | 2003 | 14616724 |
| pseudaminic acid, the major modification on campylobacter flagellin, is synthesized via the cj1293 gene. | flagellins from campylobacter jejuni 81-176 and campylobacter coli vc167 are heavily glycosylated. the major modifications on both flagellins are pseudaminic acid (pse5ac7ac), a nine carbon sugar that is similar to sialic acid, and an acetamidino-substituted analogue of pseudaminic acid (pseam). previous data have indicated that pseam is synthesized via pse5ac7ac in c. jejuni 81-176, but that the two sugars are synthesized using independent pathways in c. coli vc167. the cj1293 gene of c. jejuni ... | 2003 | 14617187 |
| transcription of sigma54-dependent but not sigma28-dependent flagellar genes in campylobacter jejuni is associated with formation of the flagellar secretory apparatus. | we performed a genetic analysis of flagellar regulation in campylobacter jejuni, from which we elucidated key portions of the flagellar transcriptional cascade in this bacterium. for this study, we developed a reporter gene system for c. jejuni involving asta, encoding arylsulphatase, and placed asta under control of the sigma 54-regulated flgde2 promoter in c. jejuni strain 81-176. the asta reporter fusion combined with transposon mutagenesis allowed us to identify genes in which insertions abo ... | 2003 | 14617189 |
| a variant of multifocal motor neuropathy with acute, generalised presentation and persistent conduction blocks. | multifocal motor neuropathy with persistent conduction blocks is classically described as a chronic neuropathy with progressive onset, and acute forms have not previously been characterised. we report four cases of severe motor impairment with acute and generalised onset and with persistent motor conduction blocks. | 2003 | 14617715 |
| heat-stable antigen serotyping of campylobacter jejuni strains isolated from hospitalized children in athens, greece. | a hundred and twentynine campylobacter jejuni strains isolated from hospitalized children with gastroenteritis were serotyped by the heat-stable antigen scheme (hs, penner's method). isolates belonged to two different periods. group a contained strains isolated in 1987-1988 and group b contained strains which were isolated in 1998-2000. a variety of serotypes was found. serotype hs:2 was predominant, followed by the hs:4 complex and hs:1,44. many clinically important guillain-barré syndrome asso ... | 2003 | 14620946 |
| resistance patterns of campylobacter spp. strains isolated from poultry carcasses in a big swiss poultry slaughterhouse. | the aim of this study was to determine resistance patterns of strains of campylobacter spp. isolated from poultry carcasses in one of the two big swiss poultry slaughterhouses. a variety of antibiotics with clinical relevance in human and/or in veterinary medicine was tested. in addition, the results of the disc diffusion method, e-test and microdilution broth methods were compared. of the 195 campylobacter jejuni strains isolated from 195 poultry carcasses from 21 flocks, 134 strains were susce ... | 2003 | 14623389 |
| effect of temperature on viability of campylobacter jejuni and campylobacter coli on raw chicken or pork skin. | to determine growth and survival of campylobacter jejuni and campylobacter coli on chicken and pork, campylobacter spp. (10(4) cfu/cm2) were inoculated on pieces of raw, irradiated chicken or pork skin and exposed to temperatures ranging from -20 to 42 degrees c under either microaerobic or aerobic conditions. viable counts over 48 h declined 2 to 3 log cfu/cm2 at -20 degrees c and 1 to 2 log cfu/cm2 at 25 degrees c regardless of skin type, species of campylobacter, or level of oxygen. at 4 degr ... | 2003 | 14627278 |
| use of an autobioluminescent campylobacter jejuni to monitor cell survival as a function of temperature, ph, and sodium chloride. | the effects of storage temperature (4, 22, and 30 degrees c), ph (4.0 to 8.5), and sodium chloride concentration (0.25 to 7.5%, wt/vol) on the survival of a strain of campylobacter jejuni atcc 35921 with an autobioluminescent phenotype (generated from the fusion of luxcdabe genes from xenorhabdus luminescens to the c. jejuni flaa promoter) in mueller-hinton broth under aerobic conditions were determined. at comparable phs, autobioluminescent c. jejuni cells die most rapidly at 30 degrees c and m ... | 2003 | 14627279 |
| conservation of dna curvature signals in regulatory regions of prokaryotic genes. | dna curvature plays a well-characterized role in many transcriptional regulation mechanisms. we present evidence for the conservation of curvature signals in putative regulatory regions of several archaeal and eubacterial genomes. genes with highly curved upstream regions were identified in orthologous groups, based on the annotations of the cluster of orthologous groups of proteins (cog) database. cogs possessing a significant number of genes with curvature signals were analyzed, and conserved ... | 2003 | 14627810 |
| functional characterization of helicobacter pylori dnab helicase. | helicobacter pylori causes gastric ulcer diseases and gastric adenocarcinoma in humans. not much is known regarding dna replication in h.pylori that is important for cell survival. here we report the cloning, expression and characterization of h.pylori dnab (hpdnab) helicase both in vitro and in vivo. among the dnab homologs, only escherichia coli dnab has been studied extensively. hpdnab showed strong 5' to 3' helicase and atpase activity. interestingly, h.pylori does not have an obvious dnac h ... | 2003 | 14627816 |
| campylobacter jejuni enterocolitis presenting as inflammatory bowel disease. | we report a case of campylobacter jejuni enterocolitis presenting as inflammatory bowel disease in a 19-year old woman. after a useless course of corticosteroids, ceftazidime and metronidazole, she was successfully treated with erythromicin. campylobacter species represent an important cause of gastroenteritis in children and adults. the rate of campylobacter isolation is 5-6 per 100,000 persons. this rate, however, grossly understimates the actual number of campylobacter infections. in most cas ... | 2003 | 14628162 |
| novel employment of lactate dehydrogenase release from porcine aortic endothelial cells (paec) as a quantitative marker of cytotoxic activity in thermophilic campylobacter spp. from human faecal isolates, poultry and environmental sources. | the aim of this study was to employ a novel cytotoxicity assay based on primary porcine aortic endothelial cells in combination with a lactate dehydrogenase release assay to quantitatively determine differences in cytotoxin production between campylobacter jejuni, c. coli, c. lari and urease-positive thermophilic campylobacters (uptc), isolated from human faeces, animals and environmental sources. campylobacter isolates totalling 34 and comprising of c. jejuni (n = 24) c. coli (n = 5) and uptc ( ... | 2003 | 14628998 |
| isolation of campylobacter jejuni and campylobacter coli from the gall bladder samples of sheep and identification by polymerase chain reaction. | in this study, 100 gall bladder samples of sheep slaughtered at an abattoir in elaziğ province were examined for campylobacter jejuni and campylobacter coli by culture and polymerase chain reaction (pcr). preston campylobacter agar supplemented with 7% horse blood and preston selective supplement (oxoid, hampshire, uk) were used for isolation of the agents. campylobacter spp. were isolated in 66 samples, and they were identified as 34% c. jejuni and 32% c. coli. a multiplex pcr based upon the us ... | 2003 | 14629001 |
| inter-laboratory evaluation of three flagellin pcr/rflp methods for typing campylobacter jejuni and c. coli: the campynet experience. | to compare typeability, discriminatory ability, and inter-laboratory reproducibility of three flagellin pcr/rflp (fla typing) methods previously described for campylobacter. | 2003 | 14633007 |
| detection and survival of campylobacter in chicken eggs. | campylobacter jejuni, a food-borne human pathogen, is widespread in poultry; however, the sources of infection and modes of transmission of this organism on chicken farms are not well understood. the objective of this study was to determine if vertical transmission of c. jejuni occurs via eggs. | 2003 | 14633036 |
| promiscuity of mhc class ib-restricted t cell responses. | murine infection with the gram-positive intracellular bacterium listeria monocytogenes activates cd8(+) t cells that recognize bacterially derived n-formyl methionine peptides in the context of h2-m3 mhc class ib molecules. three peptides, fmigwii, fmivil, and fmivtlf, are targets of l. monocytogenes-specific cd8(+) t cells. to investigate epitope cross-recognition by h2-m3-restricted cd8(+) t cells, we deleted the sequence encoding fmigwii from a virulent strain of l. monocytogenes. infection w ... | 2003 | 14634106 |
| ribosomal protection proteins and their mechanism of tetracycline resistance. | 2003 | 14638464 | |
| antimicrobial resistance in campylobacter jejuni and campylobacter coli strains isolated in 1991 and 2001-2002 from poultry and humans in berlin, germany. | the susceptibilities of 430 campylobacter jejuni strains and 79 c. coli strains to six antimicrobial agents were tested and analyzed. the two sets of strains originated from retail market chicken and turkey samples and from humans, respectively, in berlin, germany. two groups of isolates, one dating from 1991 and the other dating from 2001-2002, were tested. of the campylobacter sp. isolates recovered from humans in 2001-2002, 45.1% were resistant to ciprofloxacin, 37.8% were resistant to tetrac ... | 2003 | 14638490 |
| effects of quorum sensing on flaa transcription and autoagglutination in campylobacter jejuni. | some bacteria can communicate with other species of bacteria by means of autoinducer-2 (ai-2)-mediated quorum sensing. in this study, we demonstrated that ai-2-mediated quorum sensing influences the transcription of flaa, the major flagellin gene in campylobacter jejuni. a null mutation of luxs in c. jejuni strain 81116 reduced flaa transcription (approximately 43% that of the wild-type) and induced a reduction in motility. however, the luxs mutant had the same level of total flagellin protein a ... | 2003 | 14638994 |
| a dna element recognised by the molybdenum-responsive transcription factor mode is conserved in proteobacteria, green sulphur bacteria and archaea. | the transition metal molybdenum is essential for life. escherichia coli imports this metal into the cell in the form of molybdate ions, which are taken up via an abc transport system. in e. coli and other proteobacteria molybdenum metabolism and homeostasis are regulated by the molybdate-responsive transcription factor mode. | 2003 | 14641908 |
| heat resistance in liquids of enterococcus spp., listeria spp., escherichia coli, yersinia enterocolitica, salmonella spp. and campylobacter spp. | the aim of the work was to collect, evaluate, summarize and compare heat resistance data reported for campylobacter, enterococcus, escherichia, listeria, salmonella and yersinia spp. the work was limited to resistance in liquids with ph values 6-8. results obtained under similar experimental conditions were sought. thermal destruction lines for the various bacterial groups studied were constructed using log10 d values and treatment temperatures. there was a good linear relationship between log10 ... | 2003 | 14650540 |
| risk factors for sporadic campylobacter jejuni infections in rural michigan: a prospective case-control study. | this case-control study investigated risk factors for campylobacteriosis in a rural population. exposure to live farm animals was hypothesized to increase the risk for campylobacter jejuni enteritis. | 2003 | 14652344 |
| [use of aflp analysis for the molecular biological fine typing of campylobacter jejuni strains from turkey flocks]. | one of the most promising genotyping methods for microorganisms is the aflp (amplified fragment length polymorphism)-analysis, originally developed to genotype plants. the aflp method has a high discriminatory power and an excellent reproducibility. thus it is suitable for solving taxonomic and epidemiological questions. aflp analysis was used to reveal the genetic diversity of campylobacter (c.) jejuni strains from turkeys. the aim was to detect and genotype c. jejuni strains on individual turk ... | 2003 | 14655622 |
| [suitability of sscp-pcr analysis for molecular detection of quinolone resistance in campylobacter jejuni]. | foodborne infections with campylobacter spp. are increasing, especially antibiotic resistant strains are emerging. quinolone resistant isolates can cause failure of therapy in severe clinical infections. molecular characterisation is needed for the detection of resistant variants of c. jejuni. therefore 23 isolates from poultry and human medicine as well as three control strains were tested for their minimal inhibitory concentration, their single-strand-conformation-polymorphism (sscp)-pcr patte ... | 2003 | 14655627 |
| the wisconsin state laboratory of hygiene and emerging enteric pathogens. | at the turn of the 20th century, typhoid fever was common in wisconsin, and was a major impetus for the establishment of the wisconsin state laboratory of hygiene (wslh) in 1903. by the 1940s, typhoid was virtually eliminated in the united states due to public health measures such as disinfection of drinking water, sewage treatment, pasteurization, and shellfish bed sanitation. however, new food and waterborne pathogens have emerged to take the place of salmonella typhi. infections with non-typh ... | 2003 | 14658567 |
| new connections in the prokaryotic toxin-antitoxin network: relationship with the eukaryotic nonsense-mediated rna decay system. | several prokaryotic plasmids maintain themselves in their hosts by means of diverse post-segregational cell killing systems. recent findings suggest that chromosomally encoded copies of toxins and antitoxins of post-segregational cell killing systems - such as the rele system - might function as regulatory switches under stress conditions. the rele toxin cleaves ribosome-associated transcripts, whereas another post-segregational cell killing toxin, pare, functions as a gyrase inhibitor. | 2003 | 14659018 |
| genetic diversity of campylobacter jejuni isolates from farm animals and the farm environment. | the genetic diversity of campylobacter jejuni isolates from farm animals and their environment was investigated by multilocus sequence typing (mlst). a total of 30 genotypes, defined by allelic profiles (assigned to sequence types [sts]), were found in 112 c. jejuni isolates originating in poultry, cattle, sheep, starlings, and slurry. all but two of these genotypes belonged to one of nine c. jejuni clonal complexes previously identified in isolates from human disease and retail food samples and ... | 2003 | 14660392 |
| survival of helicobacter pylori in a natural freshwater environment. | the mode by which helicobacter pylori, the causative agent of most gastric ulcers, is transmitted remains undetermined. epidemiological evidence suggests these organisms are waterborne; however, h. pylori has rarely been grown from potential water sources. this may be due to the ability of this organism to rapidly enter the viable but nonculturable (vbnc) state. our investigation examines the entrance of h. pylori into this state in laboratory cultures and a natural freshwater environment as wel ... | 2003 | 14660399 |
| avian reservoirs and zoonotic potential of the emerging human pathogen helicobacter canadensis. | a polyphasic identification approach was used to investigate the taxonomic position of campylobacter-like isolates recovered from barnacle geese (branta leucopsis) and canada geese (branta candensis). seven strains were selected from a collection of 21 isolates and analyzed by extensive phenotypic testing; four strains were characterized by 16s rrna gene sequence analysis. the results clearly identified the bird isolates as helicobacter canadensis, recently described as an emerging human pathoge ... | 2003 | 14660407 |
| functional refolding of the campylobacter jejuni momp (major outer membrane protein) porin by groel from the same species. | functional and structural studies of outer membrane proteins from gram-negative bacteria are frequently carried out using refolded proteins. recombinant proteins are produced in escherichia coli as inclusion bodies and then tediously refolded by dilution in buffered detergent solutions. in the present work, we obtained the refolding of momp (major outer membrane protein) from campylobacter assisted by the molecular chaperone groel. refolded momp recovered its native pore-forming activity when re ... | 2004 | 14662009 |
| risk factors for antibiotic resistance in campylobacter spp. isolated from raw poultry meat in switzerland. | the world-wide increase of foodborne infections with antibiotic resistant pathogens is of growing concern and is designated by the world health organization as an emerging public health problem. thermophilic campylobacter have been recognised as a major cause of foodborne bacterial gastrointestinal human infections in switzerland and in many other countries throughout the world. poultry meat is the most common source for foodborne cases caused by campylobacter. because all classes of antibiotics ... | 2003 | 14662013 |
| risk factors associated with campylobacter jejuni infections in curaçao, netherlands antilles. | a steady increase in the incidence of guillain-barré syndrome (gbs) with a seasonal preponderance, almost exclusively related to campylobacter jejuni, and a rise in the incidence of laboratory-confirmed campylobacter enteritis have been reported from curaçao, netherlands antilles. we therefore investigated possible risk factors associated with diarrhea due to epidemic c. jejuni. typing by pulsed-field gel electrophoresis identified four epidemic clones which accounted for almost 60% of the infec ... | 2003 | 14662945 |
| molecular evidence for dissemination of unique campylobacter jejuni clones in curaçao, netherlands antilles. | campylobacter jejuni isolates (n = 234) associated with gastroenteritis and the guillain-barré syndrome (gbs) in the island of curaçao, netherlands antilles, and collected from march 1999 to march 2000 were investigated by a range of molecular typing techniques. data obtained by pulsed-field gel electrophoresis (pfge), amplified fragment length polymorphism (aflp) analysis, multilocus sequence typing (mlst), automated ribotyping, and sequence analysis of the short variable region of the flagelli ... | 2003 | 14662946 |
| multilocus sequence typing of candida glabrata reveals geographically enriched clades. | the haploid pathogenic yeast candida glabrata is the second most common candida species isolated from cases of bloodstream infection. the clinical relevance of c. glabrata is enhanced by its reduced susceptibility to fluconazole. despite this, little is known of the epidemiology or population structure of this species. we developed a multilocus sequence typing (mlst) scheme for c. glabrata and used it to fingerprint a geographically diverse collection of 107 clinical isolates and 2 reference str ... | 2003 | 14662965 |
| variation of the natural transformation frequency of campylobacter jejuni in liquid shake culture. | natural transformation, a mechanism that generates genetic diversity in campylobacter jejuni, was studied in a novel liquid shake culturing system that allowed an approximately 10 000-fold increase in cell density. c. jejuni transformation frequency was analysed in this system under 10 %, 5.0 % and 0.7 % co(2) atmospheres. at 5.0 % and 10 % co(2) concentrations, when purified isogenic chromosomal dna was used to assess competence, transformation frequency ranged from 10(-3) to 10(-4) at low cell ... | 2003 | 14663092 |
| molecular basis of bacterial outer membrane permeability revisited. | gram-negative bacteria characteristically are surrounded by an additional membrane layer, the outer membrane. although outer membrane components often play important roles in the interaction of symbiotic or pathogenic bacteria with their host organisms, the major role of this membrane must usually be to serve as a permeability barrier to prevent the entry of noxious compounds and at the same time to allow the influx of nutrient molecules. this review summarizes the development in the field since ... | 2003 | 14665678 |
| comparison of three methods for epidemiological typing of campylobacter jejuni and c. coli. | a total of 168 campylobacter strains (154 c. jejuni and 14 c. coli) isolated from human clinical samples and chicken meat were typed using penner serotyping, randomly amplified polymorphic dna (rapd), and pulsed-field gel electrophoresis (pfge) with four restriction enzymes (sac ii, sal i, sma i, kpn i). the 168 strains were found to represent 13 different penner-types and 72 different rapd-types. however, the discriminatory potential of pfge was dependent on the restriction enzymes used. the 16 ... | 2003 | 14669910 |
| direct microscopic observation and viability determination of campylobacter jejuni on chicken skin. | a method was developed to determine the survival of campylobacter jejuni at specific sites on chicken skin, and this method was used to observe the survival of c. jejuni at various locations on the skin during storage. this method uses confocal scanning laser microscopy to visualize c. jejuni transformed with p(c)gfp plasmid (gfp-campylobacter) and stained with 5-cyano-2,3-ditolyl tetrazolium chloride (ctc). the green fluorescence of dead c. jejuni cells and the red fluorescent ctc-formazan in v ... | 2003 | 14672217 |
| transfer of salmonella and campylobacter from stainless steel to romaine lettuce. | the degree of transfer of campylobacter jejuni and salmonella enterica serovar typhimurium was evaluated from a stainless steel contact surface to a ready-to-eat food (lettuce). stainless steel coupons (25 cm2) were inoculated with a 20-microl drop of either c. jejuni or salmonella typhimurium to provide an inoculum level of approximately 10(6) cfu/28 mm2. wet and dry lettuce (lactuca sativa var. longifolia) pieces (9 cm2) were placed onto the inoculated stainless steel surface for 10 s after th ... | 2003 | 14672218 |
| antagonistic activities of several bacteria on in vitro growth of 10 strains of campylobacter jejuni/coli. | chicken meat contaminated with campylobacter jejuni can be the source of human enteritis. to decrease the risk of human infection, campylobacter should be controlled at farm levels. orally given probiotic bacteria could prevent colonisation of chicken with pathogenic bacteria like campylobacter. the aim of this study was to investigate the effect of different bacteria on campylobacter growth. our results demonstrated that bacteria isolated from conventional chicken had potential inhibitory activ ... | 2004 | 14672829 |
| applicability of a rapid duplex real-time pcr assay for speciation of campylobacter jejuni and campylobacter coli directly from culture plates. | a rapid duplex real-time polymerase chain reaction (pcr) assay for speciation of campylobacter jejuni and campylobacter coli using the abi prism 7700 sequence detection system (applied biosystems) was developed based on two of the genes used in a conventional multiplex pcr. a rapid turnaround time of 3 h was achieved with the use of boiled cell lysates. applicability of the assay was tested with 6015 random campylobacter strains referred to the campylobacter reference unit, with 97.6% being iden ... | 2003 | 14680705 |
| a novel method for rapidly diagnosing the causes of diarrhoea. | the microbiological diagnosis of infectious diarrhoea may take several days using conventional techniques. in order to determine whether flatus can be used to make a rapid diagnosis, the volatile organic compounds associated with diarrhoea were analysed. | 2004 | 14684577 |
| [acute diarrhea in children less than 3 years of age: enteropathogens isolated in patient's stools, compared with a control group] | objectives: to observe the occurrence of different etiological agents of acute diarrhea (ad) in stool specimens of patients and children in a control group. material and methods: 100 children less than three years of age with ad were studied as well as 100 controls, between november 1993 and may 1994. stool specimens were collected in both groups and the following enteropathogens were searched for: rotavirus, escherichia coli (epec, etec, eiec, ehec), salmonella sp, shigella sp, campylobacter je ... | 1999 | 14685548 |
| chicken juice, a food-based model system suitable to study survival of campylobacter jejuni. | the purpose of this study was to develop a food-based model system that resembles the environment that campylobacter jejuni experiences on raw poultry products and use this model system to investigate growth and survival of the bacterium. | 2004 | 14687218 |
| l-serine catabolism via an oxygen-labile l-serine dehydratase is essential for colonization of the avian gut by campylobacter jejuni. | campylobacter jejuni is a microaerophilic, asaccharolytic bacterium. the identity of the carbon and energy sources used by c. jejuni in vivo is unknown, but the genome sequence of strain nctc11168 indicates the presence of genes for catabolism of a limited range of amino acids, including serine. specific omission of l-serine from a defined medium containing a mixture of amino acids led to a dramatic decrease in cell yields. as c. jejuni does not have a biosynthetic serine requirement, this suppo ... | 2004 | 14688104 |
| neisseria meningitidis lipooligosaccharide structure-dependent activation of the macrophage cd14/toll-like receptor 4 pathway. | meningococcal lipopoly(oligo)saccharide (los) is a major inflammatory mediator of fulminant meningococcal sepsis and meningitis. highly purified wild-type meningococcal los and los from genetically defined mutants of neisseria meningitidis that contained specific mutations in los biosynthesis pathways were used to confirm that meningococcal los activation of macrophages was cd14/toll-like receptor 4 (tlr4)-md-2 dependent and to elucidate the los structural requirement for tlr4 activation. expres ... | 2004 | 14688118 |
| induction of interleukin-8 in t84 cells by vibrio cholerae. | the induction of interleukin-8 (il-8) in vitro has been suggested to correlate with the reactogenicity of vibrio cholerae vaccine candidates. v. cholerae vaccine candidate 638, a hemagglutinin protease/hap-defective strain, was recently reported to be well tolerated in human volunteers, suggesting a role for hap in reactogenicity. we examined the role of hap in the induction of il-8 in intestinal epithelial t84 cells. wild-type v. cholerae strains 3038 and c7258 and a vaccine candidate strain, j ... | 2004 | 14688120 |
| identifying genes for neuron survival and axon outgrowth in hirudo medicinalis. | we have studied the molecular basis of nervous system repair in invertebrate (hirudo medicinalis) nerve cells. unlike in mammals, neurons in invertebrates survive injury and regrow processes to restore the connections that they held before the damage occurred. to identify genes whose expression is regulated after injury, we have used subtractive probes, constructed from regenerating and non-regenerating ganglia from the leech hirudo medicinalis, to screen cdna libraries made from whole leech cns ... | 2004 | 14690474 |
| induction of human igm and igg anti-gm1 antibodies in transgenic mice in response to lipopolysaccharides from campylobacter jejuni. | campylobacter jejuni lipopolysaccharides (lps) are implicated in the development of autoantibodies to gm1 ganglioside in patients with neuropathy following c. jejuni infection. cjlps bears oligosaccharides that are cross reactive with gm1 ganglioside and presumably exerts its effects via molecular mimicry. to study the mechanisms that are involved in development of the autoantibody response, a transgenic mouse line was developed that expresses an igm anti-gm1 antibody derived from a patient with ... | 2004 | 14698848 |
| the genome-sequenced variant of campylobacter jejuni nctc 11168 and the original clonal clinical isolate differ markedly in colonization, gene expression, and virulence-associated phenotypes. | the genome sequence of the enteric bacterial pathogen campylobacter jejuni nctc 11168 (11168-gs) was published in 2000, providing a valuable resource for the identification of c. jejuni-specific colonization and virulence factors. surprisingly, the 11168-gs clone was subsequently found to colonize 1-day-old chicks following oral challenge very poorly compared to other strains. in contrast, we have found that the original clinical isolate from which 11168-gs was derived, 11168-o, is an excellent ... | 2004 | 14702320 |
| bickerstaff's brainstem encephalitis associated with igm antibodies to gm1b and galnac-gd1a. | this is the first report of a case of bickerstaff's brainstem encephalitis (bbe) associated with igm antibodies to gm1b and galnac-gd1a. subsequent to campylobacter jejuni enteritis, the patient rapidly developed consciousness disturbance and hyperreflexia in addition to external ophthalmoplegia and cerebellar-like ataxia. eeg showed transient 7 hz monorhythmic theta activities, predominantly in the front-central area. he received high doses of immunoglobulin intravenously and had completely rec ... | 2004 | 14706228 |
| cecal colonization of chicks by bovine-derived strains of campylobacter. | campylobacter jejuni and campylobacter coli strains were isolated from feces of dairy cattle at farms with no known problem due to campylobacteria. farms were located in the northeast, desert southwest, and pacific west. twenty isolates were identified by ribotyping with a riboprinter. the ability of these bovine isolates to colonize the ceca of chicks was determined by challenge inoculation and reisolation of the challenge strain from the ceca at 1 and 2 wk after challenge. isolates recovered f ... | 2003 | 14708992 |
| antimicrobial susceptibility trends in campylobacter jejuni and campylobacter coli isolated from a rural egyptian pediatric population with diarrhea. | comparative and trend analysis was conducted on annual prevalence of antimicrobial susceptibility among campylobacter jejuni and campylobacter coli recovered from rural egyptian children from 1995 through 2000. c. jejuni and c. coli demonstrated significant decreasing trends in ciprofloxacin susceptibility over the study period (p < 0.001 for both). in general, c. coli demonstrated a higher degree of susceptibility than c. jejuni, however, there was no statistical difference (p = 0.2) comparing ... | 2003 | 14711482 |