Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| comparison of e-test and broth microdilution methods for antifungal drug susceptibility testing of molds. | we compared the e test with a broth microdilution method, performed according to national committee for clinical laboratory standards document m27-a guidelines, for determining the in vitro susceptibilities of 90 isolates of pathogenic molds (10 absidia corymbifera, 10 aspergillus flavus, 10 aspergillus fumigatus, 10 aspergillus niger, 10 aspergillus terreus, 10 exophiala dermatitidis, 10 fusarium solani, 10 scedosporium apiospermum, 5 scedosporium prolificans, and 5 scopulariopsis brevicaulis). ... | 1999 | 10203509 |
| backbone dynamics of fusarium solani pisi cutinase probed by nuclear magnetic resonance: the lack of interfacial activation revisited. | the backbone dynamics of fusarium solani pisi cutinase has been studied by a variety of nuclear magnetic resonance experiments to probe internal motions on different time scales. the core of cutinase appears to be highly rigid. the binding site, including the oxyanion hole, is mobile on the microsecond to millisecond time scale, in contrast to the well-defined active site and preformed oxyanion hole elucidated by x-ray crystallography [martinez, c., de geus, p., lauwereys, m., matthyssens, g., a ... | 1999 | 10220318 |
| in vitro and in vivo experimental activities of antifungal agents against fusarium solani. | in the treatment of disseminated fusarium infections, amphotericin b either alone or in combination with flucytosine and rifampin is the drug therapy most frequently used. the efficacy of these antifungal drugs was evaluated in a murine disseminated-infection model, with five strains of fusarium solani. all the treatments were clearly ineffective. | 1999 | 10223945 |
| in vitro activity of syn-2869, a novel triazole agent, against emerging and less common mold pathogens. | the in vitro activity of syn-2869 was compared with that of amphotericin b and itraconazole. mics for 100 isolates of pathogenic molds belonging to 12 species were determined by a broth microdilution adaptation of the method recommended by the national committee for clinical laboratory standards. syn-2869 and itraconazole showed comparable, good activity against the dematiaceous molds cladophialophora bantiana, cladophialophora carrionii, exophiala dermatitidis, fonsecaea pedrosoi, phialophora p ... | 1999 | 10223947 |
| nmr studies of fusarium solani pisi cutinase in complex with phosphonate inhibitors. | the backbone dynamics of fusarium solani pisi cutinase in complex with a phosphonate inhibitor has been studied by a variety of nuclear magnetic resonance experiments to probe internal motions on different time scales. the results have been compared with dynamical studies performed on free cutinase. in solution, the enzyme adopts its active conformation only upon binding the inhibitor. while the active site ser120 is rigidly attached to the stable alpha/beta core of the protein, the remainder of ... | 1999 | 10320324 |
| effects of methylprednisolone and cyclosporine a on fungal growth in vitro. | the management of corneal transplants after mycotic keratitis often poses a therapeutic dilemma. clinicians are hesitant to use topical steroids because of their potential enhancement of fungal growth. this study seeks to evaluate the in vitro effects of methylprednisolone and cyclosporine a on the growth of various molds that often are responsible for keratomycoses. | 1999 | 10336034 |
| subcutaneous fusarium foot abscess in a renal transplant patient. | fusarium species are ubiquitous plant and grain phytopathogens that rarely cause opportunistic infections in immunocompromised patients. while disseminated fusarium infections are almost always fatal, localized infections may be responsive to a combination of systemic antibiotic therapy and surgical debridement. we present a diabetic renal transplant patient who developed a foot abscess due to fusarium solani. infection persisted despite aggressive surgical debridement and a 3-month course of in ... | 1999 | 10349541 |
| gallotannin hydrolysis by immobilized fungal mycelia in a packed bed bioreactor. | hydrolysis of gallotannin to gallic acid by immobilized mycelia of aspergillus niger mtcc 282, aspergillus fischerii mtcc 150, fusarium solani mtcc 350 and trichoderma viride mtcc 167 in a packed bed bioreactor was studied. fungal mycelia preinduced with 5 g l-1 gallotannin were immobilized in calcium alginate gel (1.5%) and the resultant beads were packed in a column to a bed volume of 175 mm3. gallotannin dissolved in distilled water was passed through the column and the eluate was recycled af ... | 1999 | 10355367 |
| transformation of aspergillus awamori by agrobacterium tumefaciens-mediated homologous recombination. | agrobacterium tumefaciens is known to transfer part of its tumor-inducing (ti) plasmid to the filamentous fungus aspergillus awamori by illegitimate recombination with the fungal genome. here, we show that when this ti dna shares homology with the a. awamori genome, integration can also occur by homologous recombination. on the basis of this finding, we have developed an efficient method for constructing recombinant mold strains free from bacterial dna by a. tumefaciens-mediated transformation. ... | 1999 | 10385327 |
| in vitro susceptibility of opportunistic fusarium spp. to essential oils. | the inhibitory effects of essential oils extracted from 10 indian plants were evaluated against five fungi. the plants used for extraction of essential oils were six species of the genus eucalyptus and ocimum basilicum, prosopis cineraria and derris indica. the fungi used in the experiments were fusarium solani, f. oxysporum, f. pallidoroseum, f. acuminatum and f. chlamydosporum. the susceptibility of the fusarium species was tested by the paper disc method and the serial dilution technique. the ... | 1999 | 10394856 |
| specific detection of fusarium species in blood and tissues by a pcr technique. | fusarium species are opportunistic nosocomial pathogens that often cause fatal invasive mycoses. we designed a primer pair that amplifies by pcr a fragment of a gene coding for the rrna of fusarium species. the dnas of the main fusarium species and neocosmospora vasinfecta but not the dnas from 11 medically important fungi were amplified by these primers. the lower limit of detection of the pcr system was 10 fg of fusarium solani dna by ethidium bromide staining. to test the ability of this pcr ... | 1999 | 10405380 |
| dna fingerprinting of fusarium solani isolates related to a cutaneous infection in a sea turtle. | random amplified polymorphic dna (rapd) fingerprinting was applied to fusarium solani from a cutaneous hyalohyphomycosis in a loggerhead sea turtle. a total of seven f. solani isolates were examined, three from culture collections and four from the turtle infection (one from the turtle's lesions and three from the sand of the tank in which the turtle was kept). the banding patterns of the isolates from culture collections were markedly different from the patterns of the isolates from both the tu ... | 1999 | 10421856 |
| fungal keratitis as an indicator of hiv infection in africa. | a 1-year hospital-based study was undertaken on 212 patients at muhimbili medical centre (mmc) from october 1994 to october 1995. the objective was to determine the aetiology of corneal ulceration. a comparison of the prevalence of hiv infection between patients with fungal keratitis and those with non-fungal keratitis was included. bacterial infection (32.1%), and mycotic infection (15.1%) were the leading causes of corneal ulceration. there were 32 patients with fungal keratitis and 180 patien ... | 1999 | 10448232 |
| tryptophan mediated photoreduction of disulfide bond causes unusual fluorescence behaviour of fusarium solani pisi cutinase. | the fluorescence signal of the single tryptophan residue (trp69) of fusarium solani pisi cutinase is highly quenched. however, prolonged irradiation of the enzyme in the tryptophan absorption band causes an increase of the tryptophan fluorescence quantum yield by an order of magnitude. by using a combination of nmr spectroscopy and chemical detection of free thiol groups with a sulfhydryl reagent we could unambiguously show that the unusual fluorescence behaviour of trp69 in cutinase is caused b ... | 1999 | 10462054 |
| antimicrobial comparison of a new multi-purpose disinfecting solution to a 3% hydrogen peroxide system. | we compared the antimicrobial activity of a new multi-purpose disinfection solution (opti-free express with aldox) to a 3% hydrogen peroxide disinfecting system. the antimicrobial ingredients in the new solution are polyquaternium-1 and myristamidopropyl dimethylamine. | 1999 | 10555736 |
| cutinase: from molecular level to bioprocess development. | this review analyzes the role of cutinases in nature and their potential biotechnological applications. the cloning and expression of a fungal cutinase, fusarium solani f. pisi, in escherichia coli and saccharomyces cerevisiae hosts are described. the three-dimensional structure of this cutinase is also analyzed and its function as a lipase is discussed and compared with other lipases. the biocatalytic applications of cutinase are described taking into account the preparation of different cutina ... | 1999 | 10556791 |
| interdigital intertrigo of the feet due to therapy-resistant fusarium solani. | we report a case of bilateral intertrigo of the third and fourth interdigital spaces of the feet in a 34-year-old immunocompetent senegalese male. a diagnosis of fusarium solani infection was made. systemic and topical therapy with terbinafine led to clinical but not mycological recovery. as this mould is potentially dangerous for immunodepressed subjects, early diagnosis and rigorous follow-up of skin diseases caused by this agent are advisable. | 1999 | 10559592 |
| microdilution susceptibility testing of amphotericin b, itraconazole, and voriconazole against clinical isolates of aspergillus and fusarium species. | we compared the activities of amphotericin b, itraconazole, and voriconazole against clinical aspergillus (n = 82) and fusarium (n = 22) isolates by a microdilution method adopted from the national committee for clinical laboratory standards (nccls-m27a). rpmi 1640 (rpmi), rpmi 1640 supplemented to 2% glucose (rpmi-2), and antibiotic medium 3 supplemented to 2% glucose (am3) were used as test media. mics were determined after 24, 48, and 72 h. a narrow range of amphotericin b mics was observed f ... | 1999 | 10565912 |
| in vitro activities of a new lipopeptide antifungal agent, fk463, against a variety of clinically important fungi. | the in vitro antifungal activity and spectrum of fk463 were compared with those of amphotericin b, fluconazole, and itraconazole by using a broth microdilution method specified by national committee for clinical laboratory standards document m27-a (national committee for clinical laboratory standards, wayne, pa., 1997). fk463 exhibited broad-spectrum activity against clinically important pathogens including candida species (mic range, <==0.0039 to 2 microg/ml) and aspergillus species (mic range, ... | 2000 | 10602723 |
| localized oral fusarium infection in an aids patient with malignant lymphoma. | we report here a case of localized oral fusarium infection in an aids patient who developed an ulceration in the soft palate. fusarium solani was identified by histopathology and culture. we believe this to be the second reported case of oral fusarium infection in a patient with haematological malignancy and the first reported association of oral fusarium infection with aids. | 1999 | 10609534 |
| an unusual pectate lyase from a bacillus sp. with high activity on pectin: cloning and characterization. | the gene pela encoding a pectate lyase from the strain bacillus sp. bp-23 was cloned and expressed in escherichia coli. the nucleotide sequence of a 1214 bp dna fragment containing pela gene was determined, revealing an orf of 666 nucleotides that encoded a protein of 23233 da. the deduced amino acid sequence of the encoded enzyme showed homology to pectate lyases a, b, c and d from fusarium solani, pel-3 and pelb from erwinia carotovora and pell from erwinia chrysanthemi. homology was also foun ... | 2000 | 10658655 |
| microbial transformation of benzosampangine. | microbial transformation studies of the synthetic antifungal alkaloid benzosampangine (1) have revealed that 1 is metabolized by a number of microorganisms. using a standard two-stage fermentation technique absidia glauca (atcc 22752), cunninghamella blakesleeana (atcc 8688a), cunninghamella species (nrrl 5695), fusarium solani f. sp. cucurbitae (csih #c-5), and rhizopogon species (atcc 36060) each produced a beta-glucopyranose conjugate of benzosampangine (2). the identity of 2 was established ... | 2000 | 10757729 |
| deduced amino-acid sequence and possible catalytic residues of a novel pectate lyase from an alkaliphilic strain of bacillus. | the nucleotide sequence of the gene for a highly alkaline, low-molecular-mass pectate lyase (pel-15) from an alkaliphilic bacillus isolate was determined. it harbored an open reading frame of 672 bp encoding the mature enzyme of 197 amino acids with a predicted molecular mass of 20 924 da. the deduced amino-acid sequence of the mature enzyme showed very low homology (< 20.4% identity) to those of known pectinolytic enzymes in the large pectate lyase superfamily (the polysaccharide lyase family 1 ... | 2000 | 10759850 |
| transgenic arabidopsis plants expressing a fungal cutinase show alterations in the structure and properties of the cuticle and postgenital organ fusions. | a major structural component of the cuticle of plants is cutin. analysis of the function of cutin in vivo has been limited because no mutants with specific defects in cutin have been characterized. therefore, transgenic arabidopsis plants were generated that express and secrete a cutinase from fusarium solani f sp pisi. arabidopsis plants expressing the cutinase in the extracellular space showed an altered ultrastructure of the cuticle and an enhanced permeability of the cuticle to solutes. in a ... | 2000 | 10810146 |
| genetic engineering of the fusarium solani pisi lipase cutinase for enhanced partitioning in peg-phosphate aqueous two-phase systems. | the fusarium solani pisi lipase cutinase has been genetically engineered to investigate the influence of c-terminal peptide extensions on the partitioning of the enzyme in peg-salt based aqueous two-phase bioseparation systems. seven different cutinase lipase variants were constructed containing various c-terminal peptide extensions including tryptophan rich peptide tags ((wp)(2) and (wp)(4)), positively ((rp)(4)) and negatively ((dp)(4)) charged tags as well as combined tags with tryptophan tog ... | 2000 | 10812184 |
| differential expression of eight chitinase genes in medicago truncatula roots during mycorrhiza formation, nodulation, and pathogen infection. | expression of eight different chitinase genes, representing members of five chitinase classes, was studied in medicago truncatula roots during formation of arbuscular mycorrhiza with glomus intraradices, nodulation with rhizobium meliloti, and pathogen attack by phytophthora megasperma f. sp. medicaginis, fusarium solani f. sp. phaseoli (compatible interactions with root rot symptoms), ascochyta pisi (compatible, symptomless), and f. solani f. sp. pisi (incompatible, nonhost interaction). in the ... | 2000 | 10875337 |
| successful treatment of fusarium solani ecthyma gangrenosum in a child with acute lymphoblastic leukemia in relapse. | 2000 | 10877182 | |
| rapid detection and identification of candida, aspergillus, and fusarium species in ocular samples using nested pcr. | a protocol for the rapid detection of fungal dna in ocular samples, derived from three species, candida albicans, aspergillus fumigatus, and fusarium solani, has been developed. two novel panfungal primers complementary to 18s rrna sequences present in all three species were designed. panfungal pcr was followed by three nested pcrs utilizing species-specific primers. pcr sensitivity ranged from 50 to 100 fg of free dna and between one and two c. albicans organisms. in addition, we also developed ... | 2000 | 10921948 |
| comparison of nccls and 3-(4,5-dimethyl-2-thiazyl)-2, 5-diphenyl-2h-tetrazolium bromide (mtt) methods of in vitro susceptibility testing of filamentous fungi and development of a new simplified method. | the susceptibility of 30 clinical isolates belonging to six different species of filamentous fungi (aspergillus fumigatus, aspergillus flavus, scedosporium prolificans, scedosporium apiospermum, fusarium solani, and fusarium oxysporum) was tested against six antifungal drugs (miconazole, voriconazole, itraconazole, ur9825, terbinafine, and amphotericin b) with the microdilution method recommended by the national committee for clinical laboratory standards (nccls) (m38-p). the mics were compared ... | 2000 | 10921957 |
| pyrithiamine resistance gene (ptra) of aspergillus oryzae: cloning, characterization and application as a dominant selectable marker for transformation. | a pyrithiamine (pt) resistance gene (ptra) was cloned from a genomic dna library prepared from a pt resistant mutant of aspergillus oryzae. it conferred high resistance to pt on an a. oryzae industrial strain as well as a. nidulans. nucleotide sequence analysis showed that the ptra gene contained one intron (58-bp) and encodes 327 amino acid (aa) residues. additionally, the deduced aa sequence has 72% and 63% identity to fusarium solani sti35 encoding a stress-inducible protein and saccharomyces ... | 2000 | 10945258 |
| fungal keratitis in a soft contact lens wearer. | to describe a case of fungal keratitis in a soft contact lens wearer. | 2000 | 10946989 |
| mixed infection caused by two species of fusarium in a human immunodeficiency virus-positive patient. | we report on a case of mixed infection caused by two species of fusarium in a human immunodeficiency virus-positive patient with lymphoma who was neutropenic due to chemotherapy. the patient showed the typical signs of a disseminated fusarial infection, with fusarium solani isolated from skin lesions and f. verticillioides isolated from blood. the report discusses how difficult it is to make an accurate diagnosis when an immunosuppressed patient is infected with more than one fungal species, esp ... | 2000 | 10970404 |
| influence of he-ne laser irradiation of soybean seeds on seed mycoflora, growth, nodulation, and resistance to fusarium solani. | laser irradiation of soybean seeds for 3 min caused a clear reduction in the number of seed-borne fungi which became more pronounced as the irradiation time was extended. pretreatment of the seeds with methylene blue, methyl red and carmine enhanced the effect of laser. rhizoctonia solani, alternaria tenuissima, cercospora kikuchii and colletotrichum truncatum were completely eliminated when the seeds were pretreated with a dye and irradiated for 10 min. seed germination was stimulated on exposu ... | 1999 | 10983234 |
| effects of ionic surfactants used in reversed micelles on cutinase activity and stability. | the effects of aqueous surfactant solutions on the kinetics and stability of cutinase from fusarium solani pisi were studied. the surfactant sodium bis[2-ethylhexyl]ester sulfosuccinic acid (aot) acts as a pseudo-competitive inhibitor within a limited concentration range relative to the hydrolysis of short-chain p-nitrophenyl esters. for higher concentrations a hyperbolic mixed inhibition takes place. a pseudo-activation of hydrolysis in presence of aot and hexadecyltrimethyl-ammonium bromide (c ... | 2000 | 11004558 |
| invasive hyalohyphomycosis due to fusarium solani in a patient with acute lymphocytic leukemia. | 2000 | 11044201 | |
| treatment of postkeratitis fusarium endophthalmitis with amphotericin b lipid complex. | the authors report the first case of fusarium solani keratitis that progressed to fungal endophthalmitis and was successfully treated with amphotericin b lipid complex (ablc). | 2000 | 11095064 |
| fusarium solani pisi cutinase. | cutinase from fusarium solani pisi has been studied extensively with respect to its structural and functional properties. the crystal structure of the enzyme was solved to high atomic resolution (1 angstrom), while data on structural dynamics have been obtained from detailed nmr studies. functional data were mainly derived from kinetic studies using substrate analogues that simplify the kinetic behaviour. the properties of wild-type cutinase are reviewed and discussed in relation with the effec ... | 2000 | 11099798 |
| a continuous membrane bioreactor for ester synthesis in organic media: i. operational characterization and stability. | the feasibility of continuous ester synthesis in a membrane bioreactor (mbr) by a recombinant cutinase from fusarium solani pisi was investigated, using the optimal conditions previously attained by medium engineering. the objective was to analyze the mbr behavior as a differential or an integral reactor. the main component of the reactor was an anisotropic ceramic membrane with 15,000 nmwco. the operating variables included the influence of substrates ratio and flow rate on the conversion degre ... | 2001 | 11114650 |
| a continuous membrane bioreactor for ester synthesis in organic media: ii. modeling of mbr continuous operation. | a model was developed to describe the conversion degree in a membrane bioreactor (mbr) for the synthesis of short-chain esters as a function of the flow rate. the transesterification reaction was catalyzed by a recombinant cutinase of fusarium solani pisi microencapsulated in reversed micelles of aot/isooctane. the differences of product concentration in permeate and retentate together with the deactivation profiles led to an enzyme distribution evaluation that describes the experimental values ... | 2001 | 11114651 |
| analysis of the dynamics of fungal communities in soil via fungal-specific pcr of soil dna followed by denaturing gradient gel electrophoresis. | a molecular method for profiling of fungal communities in soil was applied in experiments in soil microcosms, with two objectives, (1) to assess the persistence of two selected fungal species in soil, and (2) to analyze the response of the natural fungal community to a spill of sulphurous petrol in the same soil. to achieve the aims, two soil dna extraction methods, one originally designed for the direct extraction of bacterial community dna and the other one aimed to obtain fungal dna, were tes ... | 2000 | 11121612 |
| multiple conformations of catalytic serine and histidine in acetylxylan esterase at 0.90 a. | acetylxylan esterase (axeii; 207 amino acids) from penicillium purpurogenum has substrate specificities toward acetate esters of d-xylopyranose residues in xylan and belongs to a new class of alpha/beta hydrolases. the crystal structure of axeii has been determined by single isomorphous replacement and anomalous scattering, and refined at 0.90- and 1.10-a resolutions with data collected at 85 k and 295 k, respectively. the tertiary structure consists of a doubly wound alpha/beta sandwich, having ... | 2001 | 11134051 |
| a comparison of the effects of dna-damaging agents and biotic elicitors on the induction of plant defense genes, nuclear distortion, and cell death. | pea (pisum sativum l. cv alcan) endocarp tissue challenged with an incompatible fungal pathogen, fusarium solani f. sp. phaseoli or fungal elicitors results in the induction of pathogenesis-related (pr) genes and the accumulation of pisatin, a phytoalexin. essentially the same response occurs in pea tissue exposed to dna-specific agents that crosslink or intercalate dna. in this study, the effects of dna-damaging agents were assessed relative to the inducible expression of several pea pr genes: ... | 2001 | 11161032 |
| induction of cutinolytic esterase activity during saprophytic growth of cucurbit pathogens, fusarium solani f. sp. cucurbitae races one and two (nectria haematococca mpi and mpv, respectively). | cutins from fruit of cucurbita maxima and cucurbita moschata cultivars, apple and a c(16) alcohol (hexadecanol) were used to induce cutinolytic esterase activity during saprophytic growth of strains of the two cucurbit pathogens, fusarium solani f. sp. cucurbitae, race 1 (nectria haematococca mating population (mpi) and f. solani f. sp. cucurbitae, race 2 (mpv). four strains of mpv and 11 strains of mpi were were included in the study. although we were primarily interested in the two cucurbit pa ... | 2001 | 11164297 |
| cutaneous fungal infection caused by fusarium solani. | 1998 | 11189218 | |
| disinfection efficacy of contact lens care solutions against ocular pathogens. | three commercially available products labeled as multi-purpose contact lens solutions, one multi-purpose disinfecting solution, and a hydrogen peroxide system were evaluated for antimicrobial activity according to the current international organization for standardization (iso) and the u.s. food and drug administration (fda) stand-alone procedure for disinfecting products. one multi-purpose solution was selected to assess its antimicrobial activity against two human corneal isolates of pseudomon ... | 2001 | 11215601 |
| assessment of growth of fusarium solani by cyclic voltammetry and possible bioanalytical applications. | fusarium solani, the fungus isolated from polyurethane waste scraps, was studied for its voltammetric response. the peak current values were obtained for different days and were plotted against time. this electrochemical method based on the voltammetric response showed all the four phases of the growth of the fungus. the growth curve obtained matched well with the conventional methodology, which is obtained by assessing the increase of dry weight of the organisms against time. the electrochemica ... | 2000 | 11219046 |
| three-dimensional structure of the catalytic core of acetylxylan esterase from trichoderma reesei: insights into the deacetylation mechanism. | acetylxylan esterase from trichoderma reesei removes acetyl side groups from xylan. the crystal structure of the catalytic core of the enzyme was solved at 1.9 a resolution. the core has an alpha/beta/alpha sandwich fold, similar to that of homologous acetylxylan esterase from penicillium purpurogenum and cutinase from fusarium solani. all three enzymes belong to family 5 of the carbohydrate esterases and the superfamily of the alpha/beta hydrolase fold. evidently, the enzymes have diverged from ... | 2000 | 11243887 |
| antigens of fusarium solani: types and criteria for immunochemical characterization. | there are no established methods for the preparation and standardization of fusarium solani antigens. this lack of standardization makes it difficult to use these antigens in allergenic diagnostic tests. | 2000 | 11270086 |
| enzymatic, clinical and histologic evaluation of corneal tissues in experimental fungal keratitis in rabbits. | mycotic keratitis, being frequently refractive to most of the currently available antifungal therapy, continues to pose a therapeutic challenge to the clinician. in keratitis of infectious etiology stromal dissolution may be brought about by a combination of agent and host factors. an understanding of the source and nature of corneal tissue damage is essential for evolving more effective therapeutic modalities in the treatment of fungal keratitis. in the present study, we have characterized the ... | 2001 | 11273671 |
| comparison of the e-test with the nccls m38-p method for antifungal susceptibility testing of common and emerging pathogenic filamentous fungi. | the national committee for clinical laboratory standards (nccls) m38-p method describes standard parameters for testing the fungistatic antifungal activities (mics) of established agents against filamentous fungi (molds). the present study evaluated the in vitro fungistatic activities of itraconazole and amphotericin b by the e-test and the nccls m38-p microdilution method against 186 common and emerging pathogenic molds (123 isolates of aspergillus spp. [five species], 16 isolates of fusarium s ... | 2001 | 11283057 |
| phase ii study of 4-ipomeanol, a naturally occurring alkylating furan, in patients with advanced hepatocellular carcinoma. | 4-ipomeanol (ipo; nsc 394438), a naturally occurring furan isolated from common sweet potatoes (ipomoea batatas) infected with the fungus fusarium solani was the first agent to be developed by the national cancer institute based on a biochemical-biological rationale as an anticancer agent targeted specifically against lung cancer. prior to clinical development, ipo was shown to induce pulmonary toxicity in the lungs of several mammalian species because the agent is metabolized to a highly reacti ... | 2001 | 11291834 |
| polymicrobial keratitis after laser in situ keratomileusis. | to report a case of polymicrobial infectious keratitis in one eye of a patient who had undergone bilateral simultaneous laser in situ keratomileusis (lasik). | 2001 | 11310765 |
| effect of culture conditions on lipase production by fusarium solani in batch fermentation. | lipase (glycerol ester hydrolase ec 3.1.1.3.) from a brazilian strain of fusarium solani fsi has been investigated. the effect of different carbon sources and trace elements added to basal medium was observed with the aim of improving enzyme production. lipase specific activity was highest (0.45 u mg(-1)) for sesame oil. when this medium was supplemented with trace elements using olive oil, corn oil and sesame oil the lipase specific activity increased to 0.86, 1.89 and 1.64 u mg(-1), respective ... | 2001 | 11315806 |
| effect of salts and surfactants on the partitioning of fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch. | an aqueous two-phase system composed by a thermoseparating random copolymer of ethylene oxide/propylene oxide 50/50 (%w/w), breox, and hydroxypropyl starch-reppal pes 100 was evaluated for the partitioning of fusarium solani pisi recombinant cutinase. the effect of several additives on the partitioning of pure cutinase was evaluated. micelles of sodium dodecanoate provided a ten-fold increase of the partitioning coefficient (k=9) and recovery yields of 60-75%. the phase diagrams of the systems c ... | 2000 | 11321518 |
| scale-up of recombinant cutinase recovery by whole broth extraction with peg-phosphate aqueous two-phase. | a whole broth extraction using an aqueous two-phase system (atps) composed by 5% (w/w) peg 3350 and 15% (w/w) phosphate was used for the scale-up extraction and isolation of a recombinant fusarium solani pisi cutinase, an extracellular mutant enzyme expressed in saccharomyces cerevisiae, containing a fusion peptide (wp)4. the experiments were carried out at three different scales (10 ml, 1 l and 30 l). mixing time and stirrer speed were evaluated at lab scale (1 l) with two different system comp ... | 2000 | 11321521 |
| engineering the ph-optimum of a triglyceride lipase: from predictions based on electrostatic computations to experimental results. | the optimisation of enzymes for particular purposes or conditions remains an important target in virtually all protein engineering endeavours. here, we present a successful strategy for altering the ph-optimum of the triglyceride lipase cutinase from fusarium solani pisi. the computed electrostatic ph-dependent potentials in the active site environment are correlated with the experimentally observed enzymatic activities. at ph-optimum a distinct negative potential is present in all the lipases a ... | 2001 | 11334666 |
| [ocular parasitoses and mycoses: cases diagnosed in the central university hospital of sfax between 1996 and 1999]. | parasitical and fungal ophthalmic infections are polymorphic and variably severe. they are rarely reported in publications. the aim of our study has been to specify the parasitic and fungal agents isolated from the ocular samples in our district (sfax-tunisia). we surveyed retrospectively the different ocular parasitosis and mycosis diagnosed in our laboratory (sfax hospital) over a 4 year-period (1996-1999). fungal ophthalmic infections were dominated by corneal localisations: 20 cases of kerat ... | 2001 | 11346972 |
| cloning and expression analysis of nhl1, a gene encoding an extracellular lipase from the fungal pea pathogen nectria haematococca mp vi (fusarium solani f. sp. pisi) that is expressed in planta. | the filamentous fungus nectria haematococca (anamorph fusarium solani f. sp. pisi) resides in soil, and attacks pea seedlings in the area of the underground epicotyl and upper tap root, causing foot rot disease. we detected lipase activity during in vitro growth of n. haematococca. subsequently, a lipase gene was cloned and functionally characterised by heterologous expression in saccharomyces cerevisiae. the full-length cdna of 1152 bp was cloned using a 3' race-pcr approach coupled with cdna l ... | 2001 | 11361331 |
| fusarium solani major allergen peptide iv-1 binds ige but does not release histamine. | fusarium solani (fs) is an important allergen source afflicting 4% of the nasobronchial allergy patients. fus s i3596*, a 65 kda major glycoprotein allergen of fs reacts with 95% fungus sensitive patients. | 2001 | 11422158 |
| identification of a putative solanum tuberosum transcriptional coactivator up-regulated in potato tubers by fusarium solani f. sp. eumartii infection and wounding. | coadaptors or coactivators are a new class of transcription factors capable of interconnecting a regulator dna-binding protein with a component of the basal transcription machinery allowing transcriptional activation to proceed. we report the identification of a novel solanum tuberosum ssp. tuberosum putative transcription coactivator, named stmbf1 (solanum tuberosum multiprotein bridging factor 1). the stmbf1 cdna was isolated from a fusarium solani f. sp. eumartii-infected potato tuber cdna li ... | 2001 | 11454227 |
| antifungal activity of bacillus sp. isolated from compost. | four strains of bacillus isolated from lupine compost exhibited an antifungal activity against six plant fungal pathogens (rhizoctonia solani, bipolaris sorokiniana, sclerotinia sclerotiorum, trichothecium roseum, fusarium solani, fusarium oxysporum). it was significantly influenced by the composition of the cultivation media. | 2000 | 11501422 |
| in vitro antifungal activities of the new triazole ur-9825 against clinically important filamentous fungi. | we used a modified reference microdilution method (the m-38p method) to evaluate the in vitro activities of the new triazole ur-9825 in comparison with those of amphotericin b against 77 strains of opportunistic filamentous fungi. ur-9825 was clearly more active than amphotericin b against all fungi except fusarium solani and scytalidium spp. notably, ur-9825 had low mics for aspergillus fumigatus and paecilomyces lilacinus (mics at which 90% of isolates are inhibited, 0.125 microg/ml for both s ... | 2001 | 11502542 |
| dsc studies of fusarium solani pisi cutinase: consequences for stability in the presence of surfactants. | the application of cutinase from fusarium solani pisi as a fat-stain removing ingredient in laundry washing is hampered by its lack of stability in the presence of anionic surfactants. we postulate that the stability of cutinase towards anionics can be improved by mutations increasing its temperature stability. thermal unfolding as measured with dsc, appears to be irreversible, though the thermograms are more symmetric than predicted by a simple irreversible model. in the presence of taurodeoxyc ... | 2001 | 11527580 |
| use of topical clotrimazole in human keratomycosis. | twelve out of 15 patients (9 males and 6 females) with laboratory-proven fungal keratitis were treated with topical clotrimazole, a synthetic imidazole derivative. their ages ranged from 8 to 70 years. the posttreatment visual acuities were significantly better than the pretreatment visual acuities. no significant biomicroscopic signs of ocular surface toxicity were noted. the ocular condition in 3 patients (all infected with fusarium solani) deteriorated to blindness despite the antifungal ther ... | 2001 | 11528261 |
| fusariosis associated with pathogenic fusarium species colonization of a hospital water system: a new paradigm for the epidemiology of opportunistic mold infections. | we sought the reservoir of fusarium species in a hospital with cases of known fusarial infections. cultures of samples from patients and the environment were performed and evaluated for relatedness by use of molecular methods. fusarium species was recovered from 162 (57%) of 283 water system samples. of 92 sink drains tested, 72 (88%) yielded fusarium solani; 12 (16%) of 71 sink faucet aerators and 2 (8%) of 26 shower heads yielded fusarium oxysporum. fusarium solani was isolated from the hospit ... | 2001 | 11692299 |
| synergy, pharmacodynamics, and time-sequenced ultrastructural changes of the interaction between nikkomycin z and the echinocandin fk463 against aspergillus fumigatus. | we investigated the potential synergy between two cell wall-active agents, the echinocandin fk463 (fk) and the chitin synthase inhibitor nikkomycin z (nz), against 16 isolates of filamentous fungi. susceptibility testing was performed with a broth macrodilution procedure by nccls methods. the median minimal effective concentration (mec) of fk against all aspergillus species was 0.25 microg/ml (range, 0.05 to 0.5 microg/ml). for fusarium solani and rhizopus oryzae, mecs of fk were >512 microg/ml. ... | 2001 | 11709302 |
| [activity of itraconazole against clinical isolates of aspergillus spp. and fusarium spp. determined by the m38-p nccls method]. | the antifungal activity of itraconazole was studied in 101 clinical isolates of aspergillus fumigatus, a. flavus, a. niger, a. terreus, a. nidulans, a. candidus, a. glaucus, a. clavatus, fusarium solani, f. oxysporum and f. semitectum. the minimum inhibitory concentrations (mic) were determined according to the protocol of the m38-p national committee for laboratory standards (nccls) document using a microdilution method in 1640 rpmi liquid medium (visual reading at 48 and 72 h incubation). in g ... | 2001 | 11753450 |
| regulation of constitutively expressed and induced cutinase genes by different zinc finger transcription factors in fusarium solani f. sp. pisi (nectria haematococca). | cutin monomers, generated by the low levels of constitutively expressed cutinase, induce high levels of cutinase that can help pathogenic fungi to penetrate into the host through the cuticle whose major structural polymer is cutin. we cloned three highly homologous cutinase genes, cut1, cut2, and cut3, from fusarium solani f. pisi (nectria haematococca). amino acid sequence deduced from the nucleotide sequence of cut1 and cut2/3 matched with that of the peptides from cutinase 1 and cutinase 2, r ... | 2002 | 11756444 |
| detection of microbial volatile organic compounds (mvocs) produced by moulds on various materials. | twelve fungal species were screened for microbial volatile organic compounds (mvocs): aspergillus fumigatus, a. versicolor, a. niger, a. ochraceus, trichoderma harzianum, t. pseudokoningii, penicillium brevicompactum, p. chrysogenum, p. claviforme, p. expansum, fusarium solani and mucor sp. more than 150 volatile substances derived from fungal cultures have been analysed by head-space solid-phase microextraction (hs-spme). each species had a defined mvoc profile which may be subjected to conside ... | 2001 | 11759153 |
| direct product sequestration of a recombinant cutinase from batch fermentations of saccharomyces cerevisiae. | the recovery of cutinase of fusarium solani pisi produced by the yeast saccharomyces cerevisiae was studied in a fluidised bed adsorption system directly integrated with a productive fermenter (so-called direct product sequestration; dps). the relative efficiency of this system was compared with the one of a conventional purification process by discrete sequences of fermentation, broth clarification, ultrafiltration and fixed bed anion exchange chromatography. by direct product sequestration of ... | 2001 | 11787802 |
| mitosis and motor proteins in the filamentous ascomycete, nectria haematococca, and some related fungi. | among filamentous fungi, mitosis has been studied in-depth in just a few species. the mitotic apparatuses in the ascomycetous fusarium spp. are the most clearly and readily visualized in vivo within this group; fluorescent labeling is unnecessary. this superior cytological tractability has enabled detailed studies and revealing experiments that have led the way toward a more complete understanding of fungal mitosis. some of the most important discoveries include the role of half-spindles in deve ... | 2002 | 11804038 |
| evaluation of lectin staining in the diagnosis of fungal keratitis in an experimental rabbit model. | to assess the sensitivity, specificity, and reliability of peroxidase labeled lectin staining in the diagnosis of fungal keratitis in an experimental rabbit model. | 2002 | 11815751 |
| bioremediation of ddt in soil by genetically improved strains of soil fungus fusarium solani. | bioremediation of ddt in soil by genetically improved recombinants of the soil fungus fusarium solani was studied. the parent strains were isolated from soil enriched with ddd or dde (immediate anaerobic and aerobic degradation products of ddt), as further degradation of these products are slow processes compared to the parent compound. these naturally occurring strains isolated from soil, however, are poor degraders of ddt and differed in their capability to degrade its metabolites such as ddd, ... | 2001 | 11826906 |
| pyrene degradation by yeasts and filamentous fungi. | the saprotrophic soil fungi fusarium solani (mart.) sacc., cylindrocarpon didymum (hartig) wollenw, penicillium variabile sopp. and the yeasts rhodotorula glutinis (fresenius) harrison and rhodotorula minuta (saito) harrison were cultured in mineral medium with pyrene. the remaining pyrene concentrations were periodically determined during 20 incubation days, using hplc. to assess the metabolism of pyrene degradation we added 0.1 microci of [4,5,9,10] 14c-pyrene to each fungi culture and measure ... | 2002 | 11843531 |
| high probability of disrupting a disulphide bridge mediated by an endogenous excited tryptophan residue. | it is well known that ultraviolet (uv) radiation may reduce or even abolish the biological activity of proteins and enzymes. uv light, as a component of sunlight, is illuminating all light-exposed parts of living organisms, partly composed of proteins and enzymes. although a considerable amount of empirical evidence for uv damage has been compiled, no deeper understanding of this important phenomenon has yet emerged. the present paper presents a detailed analysis of a classical example of uv-ind ... | 2002 | 11847281 |
| [an autopsy case of ph1--positive acute lymphoblastic leukemia with disseminated infection of fusarium solani]. | a 56-year-old woman with ph1--positive acute lymphoblastic leukemia was admitted to our hospital for induction chemotherapy in june 1999. the patient was presented with a central scotoma of left eye during treatment course and was given diagnosis of endophthalmitis. thereafter she also developed skin induration and suffered from serious pneumonia. amphotericin b administration was started because of high titer of beta-d-glucan, but soon discontinued due to its adverse effect. blood cultures yiel ... | 2002 | 11852477 |
| cutinase-peptide fusions in thermoseparating aqueous two-phase systems. prediction of partitioning and enhanced tag efficiency by detergent addition. | it is of increasing importance to develop efficient purification methods for recombinant proteins where the number of steps can be minimised. the aim has been to establish a method for predicting the partitioning of the wild-type target protein in an aqueous two-phase system, and with this as basis, develop fusion tags and optimise the phase system for enhanced partitioning of the target protein. the surface of the lipolytic enzyme cutinase from fusarium solani pisi was investigated with a compu ... | 2002 | 11873963 |
| mycetoma caused by fusarium solani with osteolytic lesions on the hand: case report. | eumycetoma is a mycotic disease caused by saprophytic soil fungi that are usually inoculated through minor injuries. a case of mycetoma in a brazilian farmer aged 71 years is reported. this patient presented erythema and edema on the dorsal surface of the left hand with multiple crusted and cicatricial lesions. no macroscopic grains were observed. the histopathological findings showed grains consisted of numerous hyphae which stained well with gomori-grocott method. this material obtained by cut ... | 2002 | 11913759 |
| fusarium infections of the skin. | fusarium species are ubiquitous and may be found in the soil, air and on plants. fusarium species can cause mycotoxicosis in humans following ingestion of food that has been colonized by the fungal organism. in humans, fusarium species can also cause disease that is localized, focally invasive or disseminated. the pathogen generally affects immunocompromised individuals with infection of immunocompetent persons being rarely reported. localized infection includes septic arthritis, endophthalmitis ... | 2000 | 11964778 |
| substrate-regulated cyanide hydratase (chy) gene expression in fusarium solani: the potential of a transcription-based assay for monitoring the biotransformation of cyanide complexes. | the fungus fusarium solani detoxifies cyanide through induction of the cyanide hydratase gene activity (chy) in the presence of either kcn or the metal-complexed cyanides, k2ni(cn)4 or k4fe(cn)6, at ph 7.0 and 4.0 respectively. sequence analysis of the chy gene identified primers for reverse transcriptase-polymerase chain reaction (rt-pcr)-directed analysis of mrna transcripts, which demonstrated that activity correlated to the substrate-specific induction of gene expression. chy transcription w ... | 2002 | 12000318 |
| diversity in soil fungi from undisturbed and disturbed celtis tala and scutia buxifolia forests in the eastern buenos aires province (argentina). | the rhizospheric soil microfungi from a native forest (undisturbed and disturbed) were studied using soil dilution plate and soil washing methods. fungi were isolated using slightly acid and alkaline culture media. 54 taxa were isolated: 49 from undisturbed forest soil and 37 from disturbed forest soil. acremonium sp., aspergillus ustus, coemansia pectinata, doratomyces stemonitis, fusarium solani, f. oxysporum, gliocladium roseum, humicola fusco-atra, mortierella sp., penicillium lilacinum, tri ... | 2002 | 12002400 |
| in vitro activity of fk463, a novel lipopeptide antifungal agent, against a variety of clinically important molds. | the in vitro antifungal activity of fk463 against a variety of clinically important opportunistic molds was compared with amphotericin b, itraconazole and fluconazole by using the broth microdilution method m27-a specified by the national committee for clinical laboratory standards. fk463 exhibited potent activity against aspergillus species, which was superior to those of all other compounds tested. fk463 was also active against the dematiaceous fungi cladosporium trichoides, exophiala spinifer ... | 2002 | 12011539 |
| alternate conformations observed in catalytic serine of bacillus subtilis lipase determined at 1.3 a resolution. | bacillus subtilis extracellular lipase (bsl) has an exceptionally low molecular weight (19.4 kda) for a member of the lipase family. a crystallographic study was performed on bsl in order to design and produce mutant bsl that will be more suitable for industrial uses based on analysis of the three-dimensional structure. recently, the crystal structure of bsl has been determined at 1.5 a resolution [van pouderoyen et al. (2001). j. mol. biol. 309, 215-226]. in the present study, a new crystal for ... | 2002 | 12077437 |
| host recognition by pathogenic fungi through plant flavonoids. | a common characteristic among fungal pathogens of plants is that each specializes on a narrow range of specific plants as hosts. one adaptation to a specific host plant is the recognition of the host's chemicals which can be used to trigger genes or developmental pathways needed for pathogenesis. the production of characteristic flavonoids by plants, particularly those exuded from roots by legumes, appear to be used as signals for various microbes, including symbionts as well as pathogens. nectr ... | 2002 | 12083470 |
| ocular and systemic posaconazole(sch-56592) treatment of invasive fusarium solani keratitis and endophthalmitis. | 2002 | 12084760 | |
| allergenic cross-reactivity of curvularia lunata with other airborne fungal species. | curvularia lunata is an important fungus for respiratory allergic disorders. previous studies indicated cross-reactivity of curvularia with other fungi. however, the cross-reactive allergenic component (s) were not identified. the present work was carried out to study the shared allergenic components of c. lunata and others. | 2002 | 12100306 |
| canker and wilt of black locust (robinia pseudoacacia l.) caused by fusarium species. | from the pathological material of black locust trees showing symptoms of wilting of the foliage or canker of the bark the following fusarium species were isolated: fusarium avenaceum (fr.) sacc., fusarium lateritium nees., fusarium semitectum berk. & rav., fusarium solani (mart.) sacc., fusarium sulphureum schlecht. (syn.: fusarium sambucinum fuckel f. 6 wollenw.) the results of the provocation infections of one-year-old black locust seedlings showed that all of the species--except fusarium sola ... | 2002 | 12109155 |
| endogenous endophthalmitis following disseminated fungemia due to fusarium solani in a patient with acute myeloid leukemia. | we report the case of a young man with a resistant acute myeloid leukemia (aml) who developed a disseminated fungemia due to fusarium solani involving the skin and lungs, during the neutropenic phase following a chemotherapy course. despite continuous therapy with liposomal amphotericin b, he developed a bilateral endophthalmitis that rapidly evolved to complete blindness. the patient underwent two procedures of vitrectomy, with detection of f. solani in the vitreous fluid, and continued antifun ... | 2002 | 12144539 |
| analysis of phylogenetic relationship of cylindrocarpon lichenicola and acremonium falciforme to the fusarium solani species complex and a review of similarities in the spectrum of opportunistic infections caused by these fungi. | an emerging pattern of similarity in medical case reports led to a project to compare the phylogenetic affinities of two well-known tropical fungal opportunistic pathogens, cylindrocarpon lichenicola and acremonium falciforme, to members of the fusarium solani species complex. c. lichenicola and a. falciforme, despite their deviating conidial morphologies, were shown via sequencing of the ribosomal large subunit to be well instituted within a clade mainly consisting of typical f. solani strains ... | 2002 | 12149344 |
| electrophoretic karyotyping and gene mapping of seven formae speciales in fusarium solani. | chromosomal dnas of 22 strains in 7 formae speciales (f. spp.) of fusarium solani were compared by pulsed field gel electrophoresis (pfge) and gene mapping on the chromosomes. using pfge, complete separation of the full components of the genome was not attained, due to the limited resolution of large chromosomes, but 5-12 chromosomes with sizes of 0.6-5.7 mbp were resolvable for every strain. although each strain had a unique banding profile, similarity in the banding profile was noticed among s ... | 2002 | 12172966 |
| a case of melanonychia caused by fusarium solani. | 2002 | 12207614 | |
| isolation of fusarium solani from a dog: identification by molecular analysis. | a strain of fusarium solani was isolated from a dog showing many cutaneous and submucosal nodules and pyogranulomatous kidney lesions. clinical isolates from this systemic infection were identified using microscopic examination and confirmed by molecular analysis. | 2002 | 12230226 |
| only specific tobacco (nicotiana tabacum) chitinases and [beta]-1,3-glucanases exhibit antifungal activity. | different isoforms of chitinases and [beta]-1,3-glucanases of tobacco (nicotiana tabacum cv samsun nn) were tested for their antifungal activities. the class i, vacuolar chitinase and [beta]-1,3-glucanase isoforms were the most active against fusarium solani germlings, resulting in lysis of the hyphal tips and in growth inhibition. in additon, we observed that the class i chitinase and [beta]-1,3-glucanase acted synergistically. the class ii isoforms of the two hydrolases exhibited no antifungal ... | 1993 | 12231736 |
| fusarium solani keratitis following lasik for myopia. | 2002 | 12234908 | |
| bioactivation of the fungal phytotoxin 2,5-anhydro-d-glucitol by glycolytic enzymes is an essential component of its mechanism of action. | an isolate of fusarium solani, nrrl 18883, produces the natural phytotoxin 2,5-anhydro-d-glucitol (ahg). this fungal metabolite inhibited the growth of roots (150 of 1.6 mm), but it did not have any in vitro inhibitory activity. the mechanism of action of ahg requires enzymatic phosphorylation by plant glycolytic kinases to yield ahg-1,6-bisphosphate (ahg-1,6-bisp), an inhibitor of fru-1,6-bisp aldolase. ahg-1,6-bisp had an i50 value of 570 microm on aldolase activity, and it competed with fru-1 ... | 2002 | 12240991 |
| optimal testing conditions for determining mics and minimum fungicidal concentrations of new and established antifungal agents for uncommon molds: nccls collaborative study. | this collaborative three-center study evaluated nccls m38-a document testing conditions and other testing conditions for the antifungal susceptibility testing of 35 isolates of aspergillus nidulans, a. terreus, bipolaris hawaiiensis, b. spicifera, cladophialophora bantiana, dactylaria constricta, fusarium solani, paecilomyces lilacinus, scedosporium prolificans, trichoderma longibrachiatum, and wangiella dermatitidis for itraconazole, three new triazoles (voriconazole, posaconazole, and ravucona ... | 2002 | 12354880 |
| integration of production and aqueous two-phase systems extraction of extracellular fusarium solani pisi cutinase fusion proteins. | genetic engineering was integrated with the production and purification of fusarium solani pisi cutinases, in order to obtain the highest amount of enzyme activity units, after purification. an aqueous two-phase system (atps) of polyethylene glycol 3350, dipotassium phosphate and whole broth was used for the extraction of three extracellular cutinases expressed in saccharomyces cerevisiae. the production/extraction process was evaluated regarding cutinases secretion in the medium, partition beha ... | 2003 | 12413786 |
| damping-off in conifer seedling nurseries in noshahr and kelardasht. | to study the damping-off of conifer seedlings, we have collected samples from the roots of conifer seedlings (pinus nigra, picea excelsa, abieces spp, cupressus arizonica, cupressus sempervirens) from nurseries in the south of iran (noshahr and kelardasht). after disinfecting the samples, we have used standard media like pda, ma and cla. the following fungi were identified: fusarium solani, fusarium oxysporum, fusarium sambucinum, clamydosporium, rhizoctonia solani, cylindrocarpon spp., alternar ... | 2001 | 12425024 |
| resistance locus pyramids alter transcript abundance in soybean roots inoculated with fusarium solani f.sp. glycines. | soybean sudden death syndrome (sds) is caused by fusarium solani f.sp. glycines (fsg). six quantitative trait loci (qtls), each conferring partial resistance to sds, have been discovered in an essex x forrest recombinant inbred line (ril) population, but their mode of action is not clear. this study aimed to identify genes (ests) whose mrna transcripts were altered in abundance in soybean roots following inoculation of fsg. roots of the soybean variety forrest (four resistance alleles) were inoc ... | 2002 | 12436262 |
| partitioning of peptide-tagged proteins in aqueous two-phase systems using hydrophobically modified micelle-forming thermoseparating polymer. | genetic engineering has been used to construct hydrophobically modified fusion proteins of cutinase from fusarium solani pisi and tryptophan-containing peptides. the aim was to enhance the partitioning of the tagged protein in a novel aqueous two-phase system formed by only one water-soluble polymer. the system was based on a hydrophobically modified random copolymer of ethylene oxide (eo) and propylene oxide (po) units, hm-eopo, with myristyl groups (c(14)h(29)) at both ends. the hm-eopo polyme ... | 2002 | 12445475 |