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decoctions from citrus reticulata blanco seeds protect the uroepithelium against escherichia coli invasion.traditional usage suggests citrus reticulata blanco seeds have beneficial effects against infection. the purpose of this study was to investigate the effect of citrus reticulata on the uroepithelium and to determine the mechanisms responsible for protection against urinary tract infection (uti).201324120518
phenolic compositions and antioxidant capacities of chinese wild mandarin (citrus reticulata blanco) fruits.as one of the most important centres of origin for the genus citrus l., china is rich in wild mandarin germplasm. in this study, phenolic compounds in the peels of 14 wild mandarin genotypes native to china were determined and their antioxidant capacities were evaluated using dpph, frap, abts and orac methods. we found that nieduyeju had the highest total phenol content (51.14 mg/g dw), and wulongsuanju had the highest total flavonoid content (20.66 mg/g dw). hesperidin, the dominant flavonoid, ...201424128530
detection of mandarin in orange juice by single-nucleotide polymorphism qpcr assay.a dual-probe real time pcr (qpcr) dna-based analysis was devised for the identification of mandarin in orange juice. a single nucleotide polymorphism at the trnl-trnf intergenic region of the chloroplast chromosome was confirmed in nine orange (citrus sinensis) and thirteen commercial varieties of mandarin, including citrus reticulata and citrus unshiu species and a mandarin × tangelo hybrid. two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the du ...201424128588
characterization of the citrus genome through analysis of restriction fragment length polymorphisms.studies on the nature of restriction fragment length polymorphisms (rflps) were undertaken to characterize the citrus genome. this type of analysis has not been carried out with any other perennial crop. citrus reticulata blanco cv clementine, c. xparadisi macf. cv duncan, and an f1 hybrid (lb 1-21) were used to determine what probe/enzyme combinations revealed polymorphisms in southern analysis, and a backcross family (lb 1-21x'clementine') of 65 randomly selected hybrid seedlings was used for ...199624166267
somatic hybridization between citrus reticulata and citropsis gabunensis through electrofusion.protoplasts from embryogenic calli of citrus reticulata blanco cv. ponkan and citropsis gabunensis (engl.) swing. & m. kell (cabon cherry orange), were isolated and fused using electric current. maximum fusion frequency was obtained with ac at 75 kv/cm (1.0 mhz) for 15 s, followed by dc square-wave pulses at 1.25 kv/cm for 40 μs. fusion-treated protoplasts were cultured on mt medium containing no growth regulators, solidified with 0.6% bacto difco agar. protoplast-derived calli were proliferated ...199424194127
[analysis on component difference in citrus reticulata before and after being processed with salt by uplc-q-tof/ms].to analyze components of citrus reticulata and salt-processed c. reticulata by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (uplc-q-tof/ms), and compared the changes in components before and after being processed with salt. principal component analysis (pca) and partial least squares discriminant analysis (opls-da) were adopted to analyze the difference in fingerprint between crude and processed c. reticulata, showing increased content of erioc ...201324199563
somatic hybrid plants from sexually incompatible woody species: citrus reticulata and citropsis gilletiana.allotetraploid intergeneric somatic hybrid plants between citrus reticulata blanco cv. cleopatra mandarin and citropsis gilletiana swing. & m. kell. (common name gillet's cherry orange) were regenerated following protoplast fusion. cleopatra protoplasts were isolated from an ovule-derived embryogenic suspension culture and fused chemically with leaf-derived protoplasts of citropsis gilletiana. cleopatra mandarin and somatic hybrid plants were regenerated via somatic embryogenesis. hybrid plant i ...199024232779
karyological studies in ten species of citrus(linnaeus, 1753) (rutaceae) of north-east india.ten citrus (linnaeus, 1753) species of north-east india have been karyo-morphologically analysed. all studied species had 2n=18 chromosomes without any evidence of numerical variation. all the chromosomes were found to be of metacentric and sub-metacentric in all the species; the morphology of the chromosomes showing size difference only. symmetrical karyotype which does not have much difference in the ratio of longest to shortest chromosome in all the species was observed. three species, citrus ...201124260635
a novel macromolecular extract screened from satsuma with pro-inflammatory effect.excessive consumption of horticultural fruit is a double-edged sword with both positive and negative effects. in eastern countries, a large number of people have suffered from shang huo as a result of excessive consumption of "heating" foods, such as lychee, longan, mandarin orange, mango and civet durian. the present study adopted a step by step strategy screened the compositions with pro-inflammatory effect in satsuma fruits. the pro-inflammatory effects of all fractions were evaluated in raw ...201424336758
antiviral activity of polymethoxylated flavones from "guangchenpi", the edible and medicinal pericarps of citrus reticulata 'chachi'.the present study found that the supercritical fluid extract of "guangchenpi" possessed in vitro antiviral activity against respiratory syncytial virus (rsv). bioassay-guided isolation and identification of this extract led to obtain five active polymethoxylated flavones (1-5). cytopathic effect (cpe) reduction assay exhibited that tangeretin (2) and nobiletin (3), two major polymethoxylated flavones in the extract, possessed better anti-rsv effect comparable to the positive control ribavirin. p ...201424377463
differential response of stomata to air humidity in the parasitic mistletoe (phthirusa pyrifolia) and its host, mandarin orange (citrus resitulata).measurements of co2 and h2o exchange rate and the calculated leaf conductance of attached leaves were conducted over a range of leaf-to-air vapour pressure difference (vpd) (1.5 to 5.5 kpa) to compare the response of the parasitic mistletoe, phthirusa pyrifolia, with that of its host, the mandarin orange, citrus reticulata. seedlings of the host infected with the parasite were grown in well-watered and adequately fertilized large pots outdoors at the ciat headquarters, palmira, colombia, south a ...198624442365
anti-fungal activity of citrus reticulata blanco essential oil against penicillium italicum and penicillium digitatum.the chemical composition of citrus reticulata blanco essential oil was analysed using gc/ms. monoterpene hydrocarbons (c10h16) constituted the majority (88.96%, w/w) of the total oil. the oils dose-dependently inhibited penicillium italicum and penicillium digitatum. the anti-fungal activity of the oils against p. italicum was attributed to citronellol, octanal, citral, decanal, nonanal, β-pinene, linalool, and γ-terpinene, whereas anti-fungal activity against p. digitatum is attributed to octan ...201424491729
isolation, screening, characterization, and selection of superior rhizobacterial strains as bioinoculants for seedling emergence and growth promotion of mandarin orange (citrus reticulata blanco).mandarin orange (mo) is an important fruit crop of tropical and subtropical regions of the world. a total of 217 morphologically distinct rhizobacteria from mo orchards in 3 states of northeastern india were isolated and analyzed for 4 plant-growth-promoting (pgp) attributes: nitrogen fixation, production of indole acetic acid like substances, solubilization of phosphate, and ability to antagonize pathogenic fungi. isolates were ranked based on in-vitro-assayed pgp attributes, and 10 superior is ...201424498985
rapid identification of polymethoxylated flavonoids in traditional chinese medicines with a practical strategy of stepwise mass defect filtering coupled to diagnostic product ions analysis based on a hybrid ltq-orbitrap mass spectrometer.the methodology of stepwise mass defect filtering (mdf) approach coupled to diagnostic product ions (dpis) analysis on a hybrid linear trap quadrupole (ltq)/orbitrap mass spectrometer was the first to be established to screen and identify structural analogues from complex herbal extracts.201524596165
new depside from citrus reticulata blanco.a new depside, named depcitrus a (1), and 31 known compounds were isolated from the peels, leaves and branch barks of citrus reticulata blanco. methylation of the high polarity fractions from the branch barks and peels gave one new methylated compound named depcitrus b (14) and five known compounds. their structures were established based on spectroscopic evidence. the antioxidant, antimicrobial and cytotoxic activities of some pure compounds were evaluated.201424635118
rapid screening and identification of target constituents using full scan-parent ions list-dynamic exclusion acquisition coupled to diagnostic product ions analysis on a hybrid ltq-orbitrap mass spectrometer.a highly sensitive and effective strategy for rapid screening and identification of target constituents has been developed using full scan-parent ions list-dynamic exclusion (fs-pil-de) acquisition coupled to diagnostic product ions (dpis) analysis on a hybrid ltq-orbitrap mass spectrometer. the fs-pil-de was adopted as a survey scan to trigger the ms/ms acquisition of all the predictable constituents contained in traditional chinese medicines. additionally, dpis analysis can provide a criterion ...201424767453
sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication.cultivated citrus are selections from, or hybrids of, wild progenitor species whose identities and contributions to citrus domestication remain controversial. here we sequence and compare citrus genomes--a high-quality reference haploid clementine genome and mandarin, pummelo, sweet-orange and sour-orange genomes--and show that cultivated types derive from two progenitor species. although cultivated pummelos represent selections from one progenitor species, citrus maxima, cultivated mandarins ar ...201424908277
neuroprotective effects of citrus reticulata in scopolamine-induced dementia oxidative stress in rats.the purpose of the study was to evaluate the potential effects of citrus reticulate (mandarin) peel methanolic extract (mpme) on memory dysfunction in rats. memory impairment was produced by scopolamine (1.4 mg/kg, intraperitoneally injected). brain acetylcholinesterase enzyme (ache) activity was measured to assess the central cholinergic activity. this study also investigated the effect of scopolamine on norepinephrine, dopamine and serotonin content in rat hippocampus, striatum and cerebral co ...201424938777
genome sequence of streptomyces wadayamensis strain a23, an endophytic actinobacterium from citrus reticulata.the actinobacterium streptomyces wadayamensis a23 is an endophyte of citrus reticulata that produces the antimycin and mannopeptimycin antibiotics, among others. the strain has the capability to inhibit xylella fastidiosa growth. the draft genome of s. wadayamensis a23 has ~7.0 mb and 6,006 protein-coding sequences, with a 73.5% g+c content.201424994795
effects of plant extracts on microbial population, methane emission and ruminal fermentation characteristics in in vitro.this study was conducted to evaluate effects of plant extracts on methanogenesis and rumen microbial diversity in in vitro. plant extracts (artemisia princeps var. orientalis; wormwood, allium sativum for. pekinense; garlic, allium cepa; onion, zingiber officinale; ginger, citrus unshiu; mandarin orange, lonicera japonica; honeysuckle) were obtained from the plant extract bank at korea research institute of bioscience and biotechnology. the rumen fluid was collected before morning feeding from a ...201225049630
comparison of the effects of blending and juicing on the phytochemicals contents and antioxidant capacity of typical korean kernel fruit juices.four korean kernel fruit (apple, pear, persimmon, and mandarin orange) juices were obtained by household processing techniques (i.e., blending, juicing). whole and flesh fractions of each fruit were extracted by a blender or a juicer and then examined for phytochemical content (i.e., organic acids, polyphenol compounds). the antioxidant capacity of each juice was determined by ferric reducing antioxidant power (frap) and 2,2-diphenyl-1-picrylhydrazyl (dpph) assays. results revealed that juices t ...201425054109
isolation and characterization of putative endophytic bacteria antagonistic to phoma tracheiphila and verticillium albo-atrum.a collection of 200 bacterial isolates recovered from citrus plants (citrus limon, citrus sinensis, and citrus reticulata), medicago truncatula and laurus nobilis, was established. in vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens phoma tracheiphila and verticillium albo-atrum. isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (pgpb) abilities, as well as for the presence of nonribosomal pe ...201425074353
inhibitory effects of four plants flavonoids extracts on fatty acid synthase.fatty acid synthase (fas) had been found overexpress and hyperactive in most cancers. pharmacological inhibitors of fas activity preferentially repress cancer cell proliferation and induce cancer cell apoptosis without affecting nonmalignant fibroblasts. these made fas an excellent drug target for cancer therapy. the activity of fas in 11 different kinds of cancer cells, including esophageal carcinoma (ec109, ec8712, h5e973), gastric carcinoma (n87, bgc823), lung carcinoma (a549, 95-d), hepatoma ...200925084411
[chemical variability of essential oils in peels collected in different time from citrus reticulata 'ponkan'].to study the chemical variation of essential oils in peels collected from citrus reticulata' ponkan' in different time.201325090671
effect of the nematode biocide dbx-1003 in controlling citrus nematode infecting mandarin, and interrelationship with the co-inhabitant fungi.in a field experiment, the nematode-biocide, dbx-1003, was evaluated against the citrus nematode, tylenchulus semipenetrans infecting mandarin, citrus reticulata, dbx-1003 20% g., was applied in october 2008 at the rate 1/2 kg/tree, and root and soil samples were collected monthly until the next october, 2009. successive treatment of the same biocide was added in may 2009. for the comparison, vydate 24% l, was applied as well as non-treated check trees were left. data revealed that the biocide t ...201325151818
extraction efficiency of hydrophilic and lipophilic antioxidants from lyophilized foods using pressurized liquid extraction and manual extraction.the efficient extraction of antioxidants from food samples is necessary in order to accurately measure their antioxidant capacities. α-tocopherol and gallic acid were spiked into samples of 5 lyophilized and pulverized vegetables and fruits (onion, cabbage, satsuma mandarin orange, pumpkin, and spinach). the lipophilic and hydrophilic antioxidants in the samples were sequentially extracted with a mixed solvent of n-hexane and dichloromethane, and then with acetic acid-acidified aqueous methanol. ...201425155095
community analysis of arbuscular mycorrhizal fungi in roots of poncirus trifoliata and citrus reticulata based on ssu rdna.morphological observation of arbuscular mycorrhizal fungi (amf) species in rhizospheric soil could not accurately reflect the actual amf colonizing status in roots, while molecular identification of indigenous amf colonizing citrus rootstocks at present was rare in china. in our study, community of amf colonizing trifoliate orange (poncirus trifoliata l. raf.) and red tangerine (citrus reticulata blanco) were analyzed based on small subunit of ribosomal dna genes. morphological observation showe ...201425162057
botanicals, selective insecticides, and predators to control diaphorina citri (hemiptera: liviidae) in citrus orchards.the asian citrus psyllid (acp) diaphorina citri kuwayama vectors pathogens that cause huanglongbing (hlb) or citrus greening devastating and economically important disease present in most citrus growing regions. young citrus shoots are required for psyllid reproduction and development. during winter citrus trees produce little or no new growth. overwintering adults reproduce in spring on newly emerging shoots also attractive to other pests and beneficial insects. botanicals and relatively select ...201425205398
a survey of plants responsible for causing irritant contact dermatitis in the amathole district, eastern cape, south africa.potentially harmful plants grow almost everywhere, hence, it is not practical to eradicate them all. however, a basic understanding of adverse cutaneous reactions and the common plants that cause each type can enable vulnerable individuals to discover the source of their dermatitis and thus prevent re-exposure. the aim of this study therefore, was to document the plants responsible for irritant contact dermatitis (icd) in the eastern cape, along with their respective irritants and clinical prese ...201425311272
zinc stress induces physiological, ultra-structural and biochemical changes in mandarin orange (citrus reticulata blanco) seedlings.zinc (zn) is an essential micronutrient for higher plants; yet, at higher concentrations it is toxic. in order to explore the effect of zn stress on growth, biochemical, physiological and ultra-structural changes, 1 year old mandarin plants were grown under various zn concentrations (1, 2, 3, 4, 5, 10 15 and 20 mm) for 14 weeks. the biomass of the plants increased with increasing zn concentrations and finally declined under excess zn concentration but the prime increase was observed at 4 and 5 m ...201425320469
[impacts of land-use types on soil c mineralization and temperature sensitivity of forests in qianyanzhou, jiangxi province, china].decomposition of soil organic matter plays an important role in the regulation of carbon (c) cycles at ecosystem or regional scales, and is closely related to temperature, moisture, and land-use types. the influences of soil temperature, moisture, and land-use types on soil c mineralization in citrus reticulata and pinus elliottii forests were investigated at the qianyanzhou ecological experiment station, chinese academy of sciences, by conducting incubation experiments at 5-level temperatures ( ...201425345040
13-oxo-9(z),11(e),15(z)-octadecatrienoic acid activates peroxisome proliferator-activated receptor γ in adipocytes.peroxisome proliferator-activated receptor (ppar)γ is expressed in adipose tissue and plays a key role in the regulation of adipogenesis. pparγ activators are known to have potent antihyperglycemic activity and are used to treat insulin resistance associated with diabetes. therefore, many natural and synthetic agonists of pparγ are used in the treatment of glucose disorders. in the present study, we found that 13-oxo-9(z),11(e),15(z)-octadecatrienoic acid (13-oxo-ota), a linolenic acid derivativ ...201525425149
transcript profiling analysis of rhodosporidium paludigenum-mediated signalling pathways and defense responses in mandarin orange.to investigate the basis of inducible resistance response in postharvest mandarin orange, cdna microarray and high-performance liquid chromatography were performed to study transcriptional and metabolic changes in rhodosporidium paludigenum strain treated fruit. the microarray data mining revealed that r. paludigenum activated transcription of genes important for plant hormones, signalling transduction, stress and defensive responses in orange peel tissue. moreover, up-regulation of phenylalanin ...201525442597
characterization of three linalool synthase genes from citrus unshiu marc. and analysis of linalool-mediated resistance against xanthomonas citri subsp. citri and penicilium italicum in citrus leaves and fruits.three cdna clones from satsuma mandarin (citrus unshiu marc.) were isolated and expressed in escherichia coli. custs3-1 and custs3-2 encode linalool synthases and custs4 encodes a nerolidol/linalool synthase. transcripts of custs3-1, custs3-2 and custs4 were abundant in young fruit at 60 days after flowering (daf), flowers and leaves, respectively. treatments with xanthomonas citri subsp. citri (xcc), the causal agent of citrus canker and penicillium italicum (pi), the cause of post-harvest frui ...201425443842
transcriptome and metabolome analyses of sugar and organic acid metabolism in ponkan (citrus reticulata) fruit during fruit maturation.ponkan (citrus reticulata blanco cv. ponkan) is an important mandarin citrus in china. however, the low ratio of sugars to organic acids makes it less acceptable for consumers. in this work, three stages (s120, early development stage; s195, commercial harvest stage; s205, delayed harvest stage) of ponkan fruit were selected for study. among 28 primary metabolites analyzed in fruit, sugars increased while organic acids in general decreased. rna-seq analysis was carried out and 19,504 genes were ...201525455100
parasitoids and hyperparasitoids (hymenoptera) on aphids (hemiptera) infesting citrus in east mediterranean region of turkey.the aphids, aphid parasitoids, and hyperparasitoids found in citrus orchards, the parasitoids' and hyperparasitoids' seasonal abundance, and the plant-aphid-parasitoid relationships in hatay, osmaniye, adana, and mersin provinces of the east mediterranean region of turkey are presented in the present 2-yr study. aphidius colemani viereck, binodoxys angelicae (haliday), and lysiphlebus confusus tremblay and eady (hymenoptera: braconidae: aphidiinae) were encountered as the most common parasitoids ...201425480969
asymmetric reduction of ketones by biocatalysis using clementine mandarin (citrus reticulata) fruit grown in annaba or by ruthenium catalysis for access to both enantiomers.biocatalytic reduction of prochiral ketones using freshly ripened clementine mandarin (citrus reticulata) in aqueous medium is reported. high enantioselectivities were observed, especially for the bioreduction of indanone , tetralone , and thiochromanone with respectively 95%, 99%, and 86% enantiomeric excess (ee). enantioselective bio- and metal-catalyzed reactions were compared. chiral ruthenium catalysts afforded good asymmetric inductions (>75% ee) in most cases, enantiomeric excesses depend ...201525482318
assessment of the effectiveness of orange (citrus reticulata) peel in the recovery of nickel from electroplating wastewater.wastewater discharged from electroplating industry contains different concentrations of heavy metals, which when released into the environment pose a health hazard to human beings.201425534181
next generation haplotyping to decipher nuclear genomic interspecific admixture in citrus species: analysis of chromosome 2.the most economically important citrus species originated by natural interspecific hybridization between four ancestral taxa (citrus reticulata, citrus maxima, citrus medica, and citrus micrantha) and from limited subsequent interspecific recombination as a result of apomixis and vegetative propagation. such reticulate evolution coupled with vegetative propagation results in mosaic genomes with large chromosome fragments from the basic taxa in frequent interspecific heterozygosity. modern breedi ...201425544367
diversity in sensory quality and determining factors influencing mandarin flavor liking.we evaluated the sensory quality of 42 mandarin varieties that belong to 7 different natural subgroups: common mandarin (citrus reticulata blanco), clementine (citrus clementina hort. ex. tan), satsuma (citrus unshiu marcovitch), mediterranean mandarin (citrus deliciosa tenore), king mandarin (citrus nobilis loureiro), and mandarin hybrids, such as tangor (citrus reticulata × citrus sinensis) and tangelo (citrus reticulata × citrus paradisi). consumer flavor acceptance tests revealed wide divers ...201525559381
heat shock transcription factors expression during fruit development and under hot air stress in ponkan (citrus reticulata blanco cv. ponkan) fruit.heat shock transcription factors (hsfs) play a role in plant responses to stress. citrus is an economically important fruit whose genome has been fully sequenced. so far, no detailed characterization of the hsf gene family is available for citrus. a genome-wide analysis was carried out in citrus clementina to identify hsf genes, named cchsfs. eighteen cchsfs were identified and classified into three main clades (clades a, b and c) according to the structural characteristics and the phylogenetic ...201525596345
genetic diversity and phylogenetic analysis of citrus (l) from north-east india as revealed by meiosis, and molecular analysis of internal transcribed spacer region of rdna.the north-eastern region of india is reported to be the center of origin and rich in diversity of citrus (l.) species, where some wild and endangered species namely citrus indica, citrus macroptera, citrus latipes, citrus ichagensis and citrus assamensis exist in their natural and undisturbed habitat. in order to have comprehensive information about the extent of genetic variability and the occurrence of cryptic genomic hybridity between and within various citrus species, a combined approach inv ...201425606407
[prevention of senescence and stress by food composition].the high prevalence of dementia in aged individuals suggests that aging is the most important risk factor and that senescence further enhances dementia. we have searched for dietary factors that prevent brain senescence using a mouse model of age-related neurodegeneration (samp10). this mouse line is suitable for studying brain senescence because brain atrophy and cognitive dysfunction are observed with aging, similar to humans. the production of reactive oxygen species and oxidative damage are ...201525743897
spatial and temporal spread of citrus tristeza virus and its aphid vectors in the north western area of morocco.first report of citrus tristeza virus (ctv,closterovirus) in morocco datesback to 1961 in collections of citrus varieties. an exhaustive survey of citrus in the north of the country in 2009 revealed that ctv was spread all over the citrus production area. we attempted to evaluate the relative contribution of different aphid species in the spread of ctv disease in a citrus reticulata orchard at the loukkous region during 2 years (2012 and 2013). the overall ctv incidence estimated in the experime ...201625884375
assessment of the genetic variability amongst mandarin (citrus reticulata blanco) accessions in bhutan using aflp markers.bhutan is a small himalayan country lying within the region considered to be the origin of citrus. diverse citrus wild types grow naturally in different climates, elevations and edaphic conditions, but only mandarin is cultivated commercially. the first report of huanglongbing (also known as greening disease) in bhutan in 2003, and the threat it posed to the country's citrus orchards prompted the collection of mandarin germplasm from across the country. this paper describes the genetic diversity ...201525902849
preparation, characterization and application of molecularly imprinted monolithic column for hesperetin.the molecularly imprinted solid-phase extraction (mispe) monolithic column coupled with high-performance liquid chromatography (hplc) was firstly developed for the extraction of hesperetin in the flesh of citrus reticulata cv. chachiensis, which is a traditional chinese medicine (tcm). the molecularly imprinted polymers (mips) have been prepared by a thermal polymerization method using hesperetin as the template, acrylamide (am) as functional monomer and ethylene glycol dimethacrylamide (egdma) ...201525910048
[pharmacological superiority of nobiletin-rich citrus reticulata peel, a multicomponent drug, over nobiletin alone regarding anti-dementia action]. 201525958909
house fly (musca domestica l.) attraction to insect honeydew.house flies are of major concern as vectors of food-borne pathogens to food crops. house flies are common pests on cattle feedlots and dairies, where they develop in and feed on animal waste. by contacting animal waste, house flies can acquire human pathogenic bacteria such as escherichia coli and salmonella spp., in addition to other bacteria, viruses, or parasites that may infect humans and animals. the subsequent dispersal of house flies from animal facilities to nearby agricultural fields co ...201525970333
chemical features of pericarpium citri reticulatae and pericarpium citri reticulatae viride revealed by gc-ms metabolomics analysis.this paper introduces a detailed method to apply metabolic profiles conducting on tangerine peels (citrus reticulata 'dahongpao') at three maturity stages from july to december. principal component analysis not only demonstrated the metabolic footprints of tangerine peels during ripening but also revealed the compounds (d-limonene and linalool) that mostly contributed to it. furthermore, some other characteristic compounds were screened to further reveal the chemical features of pericarpium citr ...201525976810
ougan (citrus reticulata cv. suavissima) flavedo extract suppresses cancer motility by interfering with epithelial-to-mesenchymal transition in skov3 cells.ougan (citrus reticulata cv. suavissima) flavedo extract (ofe) exhibited potential anti-tumor effects with unclear underlying mechanisms. this study aims to evaluate the potential anti-metastatic activities of ofe on human ovarian cancer cells, and investigate its inhibitory effect on epithelial-to-mesenchymal transition (emt).201526131016
biological control of toxigenic citrus and papaya-rotting fungi by streptomyces violascens mt7 and its extracellular metabolites.an indian indigenous, loktak lake soil isolate streptomyces violascens mt7 was assessed for its biocontrol potential both in vitro and in vivo against toxigenic fruit-rotting fungi. strain mt7 exhibited broad-spectrum antifungal activity against various pathogenic postharvest fungi of citrus and papaya. in shake-flask fermentation, antagonist s. violascens mt7 highly produced extracellular antifungal metabolites in early stationary growth phase in glucose-yeast extract-malt extract (m93) broth. ...201526214840
cold tolerance of the mediterranean fruit fly in date and mandarin.the mediterranean fruit fly, ceratitis capitata (wiedemann), is an endemic pest in israel and there can be low levels of infestation of dates (phoenix dactylifera l.). because c. capitata is considered a quarantine pest by several major importing countries, the export of fresh dates requires the elimination of this pest. for mandarin, cold storage at 1.11°c for 15 d is considered to be an effective treatment for the elimination of c. capitata. in this study, we compared the cold tolerance of c. ...201426309262
distinct carotenoid and flavonoid accumulation in a spontaneous mutant of ponkan (citrus reticulata blanco) results in yellowish fruit and enhanced postharvest resistance.as the most important fresh fruit worldwide, citrus is often subjected to huge postharvest losses caused by abiotic and biotic stresses. as a promising strategy to reduce postharvest losses, enhancing natural defense by potential metabolism reprogramming in citrus mutants has rarely been reported. the yellowish spontaneous mutant of ponkan (citrus reticulata blanco) (yp) was used to investigate the influence of metabolism reprogramming on postharvest performance. our results show that reduced xa ...201526329679
in vivo induction of tetraploid in tangerine citrus plants (citrus reticulata blanco) with the use of colchicine.this in vivo experiment was carried out at sakhon nakhon rajabhat university, sakhon nakhon province, thailand during march-october 2013. the study aims to search for some possibilities in inducing a large number of tetraploid sets of chromosomes in tangerine citrus seedlings with the use of colchicine chemical. a randomized complete block design (rcbd) with four replications was used. seeds of tangerine citrus were treated with colchicine solutions. the experiment consisted of seven treatments, ...201526353415
flavonoid fraction of citrus reticulata juice reduces proliferation and migration of anaplastic thyroid carcinoma cells.effects of flavonoids extracted from citrus reticulata (mandarin) juice on proliferation and migration of 3 human anaplastic thyroid carcinoma (atc) cell lines were evaluated. flavonoid components of mandarin juice extract (mje) were analyzed by uhplc. proliferation of cal-62, c-643, and 8505c cells, measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, was significantly reduced by mje in a concentration- and time-dependent way, with maximal effect elicited at 0.5 mg/ml conce ...201526365817
patterns and drivers of soil respiration under long-term citrus reticulate in southern china.soil respiration (rs) is a major source of carbon emission in terrestrial ecosystems. despite the fact that the influence of land use practice on rs has been widely studied, the patterns and drivers on rs of citrus reticulata cultivation, a worldwide land use practice are unclear. in this current study, we investigated the influence of long-term cultivation of citrus reticulata (co) and of co intercropped with soybean (cb) on soil nutrients, water availability, and rs in southern china. results ...201526368561
an examination on the modern significance of "yakushokudougen" in transferring to organic agriculture.this study attempts to identify whether and why the difference in corruption progress exists between organic and conventional farm products by conducting two corruption experiments of farm products and separation experiment of bacteria, as well as farmer survey. the results of corruption experiments for wenzhou mandarin oranges (citrus unshiu) and polished rice showed that conventional farm products demonstrated fast-growing corruption with strong unpleasant smell distinctively different from or ...201526370525
crwskp1, an skp1-like gene, is involved in the self-incompatibility reaction of "wuzishatangju" (citrus reticulata blanco).plant s-phase kinase-associated protein 1 (skp1) genes play crucial roles in plant development and differentiation. however, the role of skp1 in citrus is unclear. herein, we described a novel skp1-like gene, designated as crwskp1, from "wuzishatangju" (citrus reticulata blanco). the cdna sequence of crwskp1 is 779 base pairs (bp) and contains an open reading frame (orf) of 477 bp. the genomic sequence of the crwskp1 gene is 1296 bp with two exons and one intron. crwskp1 has high identity with s ...201526370985
determination of the precise sequences of computationally predicted mirnas in citrus reticulata by mir-race and characterization of the related target genes using rlm-race.micrornas play vital roles in various biological and metabolic processes by regulating the expression of their target genes in model plants. since there are limited reports on mirnas in citrus reticulata (crt-mirnas), the determination of precise sequences of mirnas is essential to further analyze the functions of mirnas in citrus reticulata. here, mir-race, a recently developed technique for determination of the potential mirnas computationally, was employed to identify the precise sequences of ...201626385323
pathogen infection drives patterns of nutrient resorption in citrus plants.nutrient resorption processes in the plants infected by pathogen remain poorly understood. huanglongbing (hlb) is a destructive disease of citrus. hlb-pathogen 'candidatus liberibacter asiaticus' grows specifically in the phloem of hosts and may cause problems in the plant vascular system after infection. therefore, it brings a great concern about the phloem nutrient transport and nutrient intra-cycling in hlb-affected plants. we investigated the effects of 'ca. l. asiaticus' infection on nitrog ...201526419510
slight fermentation with lactobacillus fermentium improves the taste (sugar:acid ratio) of citrus (citrus reticulata cv. chachiensis) juice.the aim of this study was to evaluate the hypothesis that fermentation with lactobacillus fermentium, which can metabolize citric acid, could be applied in improving the taste (sugar:acid ratio) of citrus juice. during fermentation, the strain of l. fermentium can preferentially utilize citric acid of citrus (citrus reticulata cv. chachiensis) juice to support the growth without the consumption of sugar. after 6 h of fermentation with l. fermentium at 30 °c, the sugar:acid ratio of citrus juice ...201526447635
nmr relaxometry study of development of freeze damage in mandarin orange.freeze damage to citrus fruit is one of the major causes of the loss of marketable fruit for the citrus industry. because freeze damage occurs inside citrus fruit, detecting freeze damage is very challenging. this study addresses this problem by using nmr relaxometry to monitor changes at the sub-cellular level in mandarin flesh at two freezing temperatures, -4 °c and -20 °c.201626455496
development and reproduction of panonychus citri (prostigmata: tetranychidae) on different species and varieties of citrus plants.the species and varieties of citrus plants that are currently grown can favor the population growth of the citrus red mite panonychus citri (mcgregor) (prostigmata: tetranychidae) and alter the pest management programs in citrus groves. in this study we evaluated, in the laboratory, the development and reproduction of p. citri and estimated its life table parameters when reared on four varieties of citrus sinensis (l.) osbeck (valencia, pera, natal, and hamlin), one variety of citrus reticulata ...201526459376
methyl-n-methylanthranilate, a pungent compound from citrus reticulata blanco leaves.more analgesic compounds are needed in medicine against pain since the available drugs displayed secondary effects. natural products are a source of molecules to develop new analgesics, using the information of plants, applied against pain, with effects such as pungency, tingling, and needle, due to their possible role in the central nervous system (ncs). citrus reticulata blanco (rutaceae) leaves are usually bitten to flavor the mouth and possess this type effect in lips and tongues; due to thi ...201626471095
comparison of ensemble strategies in online nir for monitoring the extraction process of pericarpium citri reticulatae based on different variable selections.different ensemble strategies were compared in online near-infrared models for monitoring active pharmaceutical ingredients of traditional chinese medicine. bagging partial least square regression and boosting partial least square regression were adopted to near-infrared models, to determine hesperidin and nobiletin content during the extraction process of pericarpium citri reticulatae in a pilot scale system. different pretreatment methods were investigated, including savitzky-golay smoothing, ...201626485639
neuroprotective effect of citrus unshiu immature peel and nobiletin inhibiting hydrogen peroxide-induced oxidative stress in ht22 murine hippocampal neuronal cells.oxidative stress-induced cell damage is common in the etiology of several neurobiological disorders, including alzheimer's disease and parkinson's disease. in a case study, nobiletin-rich citrus reticulata peels could prevent the progression of cognitive impairment in donepezil-preadministered alzheimer's disease patients.201526664016
inhibitory effects of amines from citrus reticulata on bleomycin-induced pulmonary fibrosis in rats.idiopathic pulmonary fibrosis (ipf) is a progressive, fatal lung disease for which, thus far, there are no effective treatments. the pericarp of citrus reticulata, as a traditional herbal drug, has been used for the clinical treatment of lung-related diseases in china for many years. in the present study, the amines from the pericarp of citrus reticulata were isolated, and their hydrochlorides were prepared. the results of screening using cultured human embryonic lung fibroblasts (helfs) reveale ...201626675886
storage stability of kinnow fruit (citrus reticulata) as affected by cmc and guar gum-based silver nanoparticle coatings.the influence of carboxy methyl cellulose (cmc) and guargum-based coatings containing silver nanoparticles was studied on the postharvest storage stability of the kinnow mandarin (citrus reticulata cv. blanco) for a period of 120 days (85%-90% relative humidity) at 4 °c and 10 °c. physicochemical and microbiological qualities were monitored after every 15 days of storage. overall results revealed an increase in total soluble solid (tss), total sugars, reducing sugars and weight loss but this inc ...201526694344
coupling a branch enclosure with differential mobility spectrometry to isolate and measure plant volatiles in contained greenhouse settings.volatile organic compounds (vocs) are off-gassed from all living organisms and represent end products of metabolic pathways within the system. in agricultural systems, these vocs can provide important information on plant health and can ordinarily be measured non-invasively without harvesting tissue from the plants. previously we reported a portable gas chromatography/differential mobility spectrometry (gc/dms) system that could distinguish voc profiles of pathogen-infected citrus from healthy t ...201626695246
oxidative burst inhibition, cytotoxicity and antibacterial acriquinoline alkaloids from citrus reticulate (blanco).two novel acridone-quinoline alkaloids, acriquinoline a (1) and acriquinoline b (2), together with twenty-two known compounds were isolated from the methanol extract of the root of citrus reticulata blanco. the structures of all compounds were determined by comprehensive analyses of their 1d and 2d nmr and mass spectral (ei and esi) data. the possible biosynthesis for the formation of above compounds is proposed, based on close examination of their structures. compounds 1, 2, 6, 10 and 14-17 exh ...201626711890
identification of novel and conserved mirnas in leaves of in vitro grown citrus reticulata "lugan" plantlets by solexa sequencing.micrornas (mirnas) play essential roles in plant development, but the roles in the in vitro plant development are unknown. leaves of ponkan plantlets derived from mature embryos at in vitro culture conditions were used to sequence small rna fraction via solexa sequencing, and the mirnas expression was analyzed. the results showed that there were 3,065,625 unique sequences in ponkan, of which 0.79% were mirnas. the rna sequences with lengths of 18-25 nt derived from the library were analyzed, lea ...201526779240
the use of aromasticks to help with sleep problems: a patient experience survey.to document the use of aromasticks to facilitate sleep in a cancer centre in the uk. sleep disturbance is a common problem amongst patients diagnosed with cancer. essential oils may be inhaled by means of an aromastick (a personal inhaler device containing essential oils) as a means of improving sleep. a prospective audit of aromasticks given to help facilitate sleep. sixty-five aromasticks were given out over a 13 week period. 94% of patients reported that they did use their aromastick to help ...201626850806
first report of lecanodiaspis dendrobii douglas, 1892 (hemiptera: lecanodiaspididae) and the associated parasitoid cephaleta sp. (hymenoptera: pteromalidae) in brazil.lecanodiaspis dendrobii douglas, 1892 (hemiptera: coccoidea: lecanodiaspididae) and the associated parasitoid cephaleta sp. (hymenoptera: pteromalidae) are reported for the first time in brazil. specimens of this scale insect were collected on branches and stems of acacia mangium willd., leucaena leucocephala (lam.) de wit (fabaceae), morus nigra l. (moraceae), citrus reticulata blanco (rutaceae), tectona grandis l. f. (verbenaceae), anacardium occidentale (anacardiaceae), annona squamosa l. and ...201626871743
the influence of negative urgency, attentional bias, and emotional dimensions on palatable food consumption.we tested a theoretical model concerning the role of attentional bias and negative affect in food consumption that offers important advances. we hypothesized that the effects of negative affect manipulations on food consumption vary as a function of trait levels of negative urgency (nu; tendency to act impulsively when distressed), and attentional bias and that the roles of emotional arousal and negative emotional valence differ and should be studied separately. 190 undergraduate women were rand ...201626877214
prevention of obesity and type 2 diabetes with aged citrus peel (chenpi) extract.chenpi is the dry peel of the plant citrus reticulata blanco after an aging processing. it has been used as an antidigestive and anti-inflammatory traditional medicine, as well as culinary seasoning and dietary supplement, in china. however, its efficacy and underlying scientific mechanism have not been sufficiently investigated. chenpi is uniquely enriched with a high content of 5-demethylated polymethoxyflavones (5-oh pmfs). the effect of chenpi extract on improving metabolic features was exam ...201626912037
metals in agricultural produce associated with acid-mine drainage in mount morgan (queensland, australia).acid-mine drainage (amd) into the dee river from the historic gold and copper mine in mount morgan, queensland (australia) has been of concern to farmers in the area since 1925. this study sought to determine the levels of amd-related metals and sulfur in agricultural produce grown near the mine-impacted dee river, compare these with similar produce grown in reference fields (which had no known amd influence), and assess any potential health risk using relevant australian or us guidelines. analy ...201626979303
anti‑inflammatory and antioxidant activity of the traditional herbal formula gwakhyangjeonggi‑san via enhancement of heme oxygenase‑1 expression in raw264.7 macrophages.gwakhyangjeonggi‑san (ghjgs) is a mixture of herbal plants, including agastache rugosa, perilla frutescens, angelica dahurica, areca catechu, poria cocos, magnolia officinalis, atractylodes macrocephala, citrus reticulata, pinellia ternata, platycodon grandiflorum, glycyrrhiza uralensis, ziziphus jujuba and zingiber officinale. ghjgs has been used for treating diarrhea‑predominant irritable bowel syndrome in traditional korean medicine. in the present study, the anti‑inflammatory and antioxidant ...201627052497
alterations of physiology and gene expression due to long-term magnesium-deficiency differ between leaves and roots of citrus reticulata.seedlings of ponkan (citrus reticulata) were irrigated with nutrient solution containing 0 (mg-deficiency) or 1mm mgso4 (control) every two day for 16 weeks. thereafter, we examined magnesium (mg)-deficiency-induced changes in leaf and root gas exchange, total soluble proteins and gene expression. mg-deficiency lowered leaf co2 assimilation, and increased leaf dark respiration. however, mg-deficient roots had lower respiration. total soluble protein level was not significantly altered by mg-defi ...201627163764
metabolic variations in different citrus rootstock cultivars associated with different responses to huanglongbing.huanglongbing (hlb) is one of the most destructive bacterial diseases of citrus. no resistant cultivars have been identified, although tolerance has been observed in the genus poncirus and some of its hybrids with citrus that are commonly used as rootstocks. in this study we exploited this tolerance by comparing five different tolerant hybrids with a cultivar that shows pronounced hlb sensitivity to discern potential contributing metabolic factors. whole leaves of infected and non-infected green ...201627236226
low temperature induced changes in citrate metabolism in ponkan (citrus reticulata blanco cv. ponkan) fruit during maturation.citrate is the most important organic acid in citrus fruit, and its concentration in fruit cells is regulated mainly by the balance between synthesis and degradation. ponkan (citrus reticulate blanco cv. ponkan) is one of the major citrus cultivars grew in china, and the fruit are picked before fully mature to avoid bad weather. greenhouse production is widely used to prolong the maturation period and improve the quality of ponkan fruit by maintaining adequate temperature and providing protectio ...201627249065
proteomic analysis of differentially expressed proteins involved in peel senescence in harvested mandarin fruit.mandarin (citrus reticulata), a non-climacteric fruit, is an economically important fruit worldwide. the mechanism underlying senescence of non-climacteric fruit is poorly understood. in this study, a gel-based proteomic study followed by lc-esi-ms/ms analysis was carried out to investigate the proteomic changes involved in peel senescence in harvested mandarin "shatangju" fruit stored for 18 days. over the course of the storage period, the fruit gradually senesced, accompanied by a decreased re ...201627303420
purification and characterization of arctium lappa l. (edible burdock) polyphenol oxidase.polyphenol oxidase of arctium lappa l. (edible burdock) has been purified by chromatographies on deae-cellulose, sephadex g-75, and phenyl-cellulofine to a homogeneous state as judged by sds polyacrylamide gel electrophoresis. the molecular weight of the purified enzyme was estimated to be 31,000 by sds-page and 25,000 by gel filtration on tskgel g2000sw. the optimum ph was 7.0 and the enzyme was stable at ph 7.0-9.0. the enzyme oxidized triphenols such as pyrogallol and phloroglucinol, and was ...199327314765
evaluation of skin anti-aging potential of citrus reticulata blanco peel.the peel of citrus reticulata blanco is traditionally used as tonic, stomachic, astringent, and carminative. it is also useful in skin care.201727365982
chemical composition and anti-inflammatory effects of essential oil from hallabong flower.a number of essential oils derived from plants are claimed to have several medicinal functions, including anti-cancer and anti-inflammation effects. however, the chemical composition and biological activities of flower-derived components have not been sufficiently characterized. therefore, we investigated the composition of essential oils from hallabong flower [(citrus unshiu marcov × citrus sinensis osbeck) × citrus reticulata blanco] and their anti-inflammatory effects. hydro-distilled essenti ...201327366141
discrimination of citrus reticulata blanco and citrus reticulata 'chachi' by gas chromatograph-mass spectrometry based metabolomics approach.citri reticulatae pericarpium, mainly including the pericarp of citrus reticulata blanco and the pericarp of citrus reticulata 'chachi', has been consumed daily as food and dietary supplement for centuries. in this study, gc-ms based metabolomics was employed to compare comprehensively the volatile constituents in citrus reticulata blanco and citrus reticulata 'chachi'. principal component analysis and orthogonal partial least squares discrimination analysis indicated that samples could be disti ...201627374515
protective efficacy of the ingestion of mandarin orange containing β-cryptoxanthin on lipopolysaccharide-induced acute nephritis.β-cryptoxanthin is a common carotenoid pigment found in fruit, especially in satsuma mandarins and in persimmons. after ingestion, β-cryptoxanthin is distributed to and accumulates in organs, such as the liver, lung, and kidney. recent studies have reported that because of its antioxidant defense, β-cryptoxanthin performs several important functions in the preservation of human health and in the prevention of several diseases, including cancer and osteoporosis. the present study aims to determin ...201627374966
digital gene expression analysis of ponkan mandarin (citrus reticulata blanco) in response to asia citrus psyllid-vectored huanglongbing infection.citrus huanglongbing (hlb), the most destructive citrus disease, can be transmitted by psyllids and diseased budwoods. although the final symptoms of the two main hlb transmission ways were similar and hard to distinguish, the host responses might be different. in this study, the global gene changes in leaves of ponkan (citrus reticulata) mandarin trees following psyllid-transmission of hlb were analyzed at the early symptomatic stage (13 weeks post inoculation, wpi) and late symptomatic stage ( ...201627384559
host plant affects the sexual attractiveness of the female white-spotted longicorn beetle, anoplophora malasiaca.anoplophora malasiaca (coleoptera: cerambycidae) is a serious pest that destroys various landscape and crop trees in japan. we evaluated the precopulatory responses of three different a. malasiaca populations collected from mandarin orange, willow and blueberry trees. most of the males accepted mates from within the same host plant population as well as females from the willow and blueberry populations. however, significant number of males from the blueberry and willow populations rejected femal ...201627412452
rotylenchus microstriatus n.sp. (hoplolaimidae: nematoda) from bolivia.rotylenchus microstriatus n.sp. is described from musa sp. and citrus reticulata in bolivia. it has males and is characterized by a continuous and finely striated lip region of 9 to 11 annules. ac]19820601.198327432373
a combined spectroscopic and tddft study of natural dyes extracted from fruit peels of citrus reticulata and musa acuminata for dye-sensitized solar cells.this study reports the novel spectroscopic investigations and enhanced the electron transfers of citrus reticulata and musa acuminata fruit peels as the photosensitizers for the dye-sensitized solar cells. the calculated td-dft-ub3lyp/6-31+g(d,p)-iefpcm(uaks), experiment spectra of ultra-violet-visible spectroscopy, and fourier transform infrared spectroscopy studies indicate the main flavonoid (hesperidin and gallocatechin) structures of the dye extracts. the optimized flavonoid structures are ...201727497289
novel synthesized 2, 4-dapg analogues: antifungal activity, mechanism and toxicology.2, 4-diacetylphloroglucinol (2,4-dapg), a natural phenolic compound, has been investigated in light of its biological activities against plant pathogens. to improve its potential application, fourteen 2,4-dapg analogous were synthesized through the friedel-crafts reaction using acyl chlorides and phloroglucinol. of the 2,4-dapg derivatives, mp4 exhibited much higher antifungal activity against penicillium digitatum and p. italicum, the major pathogenic fungi in citrus fruit, than 2, 4-dapg in vi ...201627562341
in vitro physicochemical, phytochemical and functional properties of fiber rich fractions derived from by-products of six fruits.a comparative study was done on the health promoting and functional properties of the fibers obtained as by-products from six fruits viz., pomace of carambola (averrhoa carambola l.) and pineapple (ananas comosus l. merr), peels of watermelon (citrullus lanatus), burmese grape (baccurea sapida muell. arg) and khasi mandarin orange (citrus reticulata blanco), and blossom of seeded banana (musa balbisiana, abb). highest yield of fiber was obtained from burmese grape peel (bgpl, 79.94 ± 0.41 g/100  ...201627570274
possible gender difference in anti-stress effect of β-cryptoxanthin.beta-cryptoxanthin [β-crx, (3r)-β, β-caroten-3-ol] is a provitamin a and a potent antioxidant that is abundant in satsuma mandarin orange (citrus unshiu marc.), which is the most popular fruit in japan. the anti-stress effect of β-crx on humans was evaluated in fifth-year university students during both routine daily life at the university and at pharmacy practice. the study design was a double-blind group comparison and participants (n=20; female 12, male 8) were randomly assigned to β-crx-rich ...201627592828
quantification of pesticides in food crops using quechers approaches and gc-ms/ms.the analysis of pesticides in food products requires accurate measurements for which standardised protocols have been developed. this paper reports the validation of quechers (quick, easy, cheap, effective, rugged, and safe)-based methods applied to three different food samples (brown rice, red pepper and mandarin orange) analysed using gas chromatography-tandem mass spectrometry (gc-ms/ms). the validation of the analytical methods used to measure 113 pesticides, including various insecticides, ...201627619377
composition by lc-ms/ms of new carotenoid esters in mango and citrus.interest in the composition of carotenoid esters of fruits is growing because esterification may affect their bioavailability. thus, the aim was to provide a detailed identification of carotenoid esters in citrus and mango. orange cv. 'valencia' and cv. 'pera' presented 9 free carotenoids, 38 monoesters, and 60 diesters. violaxanthin and luteoxanthin derivatives were the major ones, followed by antheraxanthin, lutein, zeaxanthin, β-cryptoxanthin, and zeinoxanthin esters, many of them reported fo ...201627712060
flavonoid composition of orange peel and its association with antioxidant and anti-inflammatory activities.dried citrus peel derived from citrus reticulata, also called "chenpi", possesses a complex mixture of flavonoids and has a history of traditional use to treat a variety of digestive disorders. we compared three sources of conventional chenpi from california (usa), guangxi, zhejiang, and two sources of "nchenpi", which contain greater nobiletin content, from sichuan and xinhui (china). xinhui orange peel extract (ope) had highest content of polymethoxylated flavones, along with greatest capacity ...201727719891
reusable nanobiocatalysts for the efficient extraction of pigments from orange peel.nagpur mandarin orange (citrus reticulata) peels were subjected to treatment with nanobiocatalysts in the form of cellulase and pectinase immobilized magnetic nanoparticles (mnps). mnps (fe3o4) with average diameter in range of 40-90 nm were immobilized with cellulase and pectinase through aptes and glutaraldehyde. treatment followed by extraction into organic solvents resulted in 8-9 fold increase in extraction of carotenoidic pigments compared to use of free enzymes. optimum ph and temperature ...201627765971
genome wide selection in citrus breeding.genome wide selection (gws) is essential for the genetic improvement of perennial species such as citrus because of its ability to increase gain per unit time and to enable the efficient selection of characteristics with low heritability. this study assessed gws efficiency in a population of citrus and compared it with selection based on phenotypic data. a total of 180 individual trees from a cross between pera sweet orange (citrus sinensis osbeck) and murcott tangor (citrus sinensis osbeck x ci ...201627813590
protective effect of tangeritin in transgenic drosophila model of parkinson's disease.parkinson's disease (pd) is a neurodegenerative disorder caused due to the loss of dopaminergic neurons in substantia nigra region of midbrain. the disease is characterized by the accumulation of alpha-synuclein into depositions known as lewy bodies. till date there is no cure for pd but the limited number of medications may provide temporary relief from the pd symptoms. flavonoids are a group of polyphenols found in plants. the health benefits of flavonoids have been universally accepted. tange ...201727814588
schistosomicidal effects of the essential oils of citrus limonia and citrus reticulata against schistosoma mansoni.we report the in vitro schistosomicidal effects of the essential oil obtained from citrus limonia leaves (cl-eo) and c. reticulata fruit peels (cr-eo), cultivated in brazil, against schistosoma mansoni worms. limonene (29.9%), β-pinene (12.0%), sabinene (9.0%), citronellal (9.0%), and citronellol (5.8%) are the major constituents of cl-eo; limonene (26.5%), γ-terpinene (17.2%), linalool (11.1%), octanal (8.0%), myrcene (6.2%), and capraldehyde (3.9%) predominate in cr-eo. cl-eo displayed moderat ...201727936310
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