Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the effect of titanium dioxide inhalation on the pulmonary clearance of pasteurella haemolytica in the mouse. | four inhalation exposure chambers were designed and built in which up to 80 mice per chamber could be accommodated. exposure for several weeks to an inert inorganic particulate aerosol of titanium dioxide at a respirable aerosol concentration of 20 mg m-3 impaired the clearance of pasteurella haemolytica in proportion to the duration of exposure. other groups exposed to a lower respirable concentration of 2 mg m-3 exhibited similar clearance rates relative to mice maintained in clean air. a reco ... | 1989 | 2792057 |
| pulmonary clearance of pasteurella haemolytica and immune responses in mice following exposure to titanium dioxide. | the effects of dust inhalation on immune responses in mice to aerosolized pasteurella haemolytica were investigated. animals exposed to titanium dioxide dust (tio2) at a respirable aerosol concentration of approximately 20 mg m-3 for 20 hr per day, before or after aerosol vaccination, had impaired pulmonary bacterial clearance relative to animals kept in clean air. additionally, lymphocytes from the local (mediastinal) lymph nodes had depressed responses to bacterial antigen in vitro in mice exp ... | 1989 | 2792059 |
| chemotactic response of bovine neutrophils to pasteurella haemolytica culture fluid. | bovine neutrophil chemotactic activity was detected in the supernatant fluid of logarithmic phase cultures of p. haemolytica serotype 1. the chemoattractant was produced under culture conditions suitable for p. haemolytica leukotoxin production. an inverse correlation existed between the leukotoxin lc50 and the chemotactic activity in the culture fluid. elimination of leukotoxin activity by heating, dilution or ultrafiltration, exposed the chemotactic activity in the culture fluid. the chemoattr ... | 1989 | 2800327 |
| effect of pasteurella haemolytica infection on the distribution of sulfadiazine and trimethoprim into tissue chambers implanted subcutaneously in cattle. | a study was designed to determine the effect of pasteurella haemolytica infection on the rate and extent of penetration of sulfadiazine and trimethoprim into tissue chambers implanted sc in cattle. thermoplastic tissue chambers were implanted sc in 6 calves. at 35 days after implantation, sulfadiazine (25 mg/kg of body weight) and trimethoprim (5 mg/kg) were administered iv to 5 of the calves. chamber fluid and blood samples were collected from each animal at various time intervals for 24 hours ... | 1989 | 2802332 |
| interaction between pasteurella haemolytica, sulfadiazine/trimethoprim, and bovine viral diarrhea virus. | a study was designed to develop and define a sc tissue chamber as a suitable device for establishing a soft-tissue infection model in cattle and to use this model to study the interaction between pasteurella haemolytica, sulfadiazine/trimethoprim, and bovine viral diarrhea virus (bvdv). thermoplastic tissue chambers were implanted in the paralumbar fossae of 20 calves. at 35 days after implantation, calves were allotted to 4 groups of equal size and the calves in 2 groups were inoculated intratr ... | 1989 | 2802333 |
| direct effects of pasteurella haemolytica lipopolysaccharide on bovine pulmonary endothelial cells in vitro. | bovine pulmonary artery cells in cell culture were exposed to lipopolysaccharide (lps) purified from pasteurella haemolytica serotype al. this resulted in severe membrane damage, which caused a time- and dose-dependent release of lactate dehydrogenase that was first detected 4 hours after exposure and reached a maximal mean release of 67% after 24 hours of exposure to 1 micrograms of lps/ml. mean release of 51chromium followed by a similar pattern and reached a maximum of 61% following 24 hours ... | 1989 | 2802342 |
| intraperitoneal inoculation of ewes with an autogenous vaccine to prevent mastitis due to pasteurella haemolytica. | 1989 | 2818367 | |
| pasteurella haemolytica serotype 1 colonization of the nasal passages of virus-infected calves. | nasal passages of calves with a virus-induced respiratory tract disease became colonized by pasteurella haemolytica serotype 1 after they were inoculated intranasally with p haemolytica. inoculation with infectious bovine rhinotracheitis virus caused a more severe clinical illness and resulted in a greater degree of colonization with p haemolytica than developed after inoculation with parainfluenza-3 virus. nasal passages of parainfluenza-3 virus-inoculated calves were colonized to a greater deg ... | 1987 | 2829666 |
| effects of smooth and rough pasteurella haemolytica lipopolysaccharides on plasma cyclic-nucleotides and free fatty acids in calves. | the present study examined the potency of smooth or rough pasteurella haemolytica lipopolysaccharide infusion (lps, 24 ng kg-1 min-1 for 500 min) on plasma cyclic-nucleotides and several free fatty acids (ffa) in calves. both smooth or rough lps increased plasma camp immediately to its maximum at 1 h of infusion, whereas plasma cgmp levels rose slowly and peaked 12 h later. the increases in camp levels were more prolonged for smooth lps than rough lps. the maximum plasma camp rise coincided with ... | 1987 | 2830707 |
| the possible involvement of immunosuppression caused by a lentivirus in the aetiology of jaagsiekte and pasteurellosis in sheep. | a south african isolate of ovine lentivirus was shown to cause a mild immunosuppression in sheep, reflected by a reduced delayed hypersensitivity reaction. this effect, measured in terms of skin swelling after intradermal inoculation with tuberculin, showed a positive linear relationship with the latency period before the appearance of jaagsiekte symptoms in animals co-infected with jsrv, as well as with the activity of monocytes. in a parallel study, increased susceptibility of lentivirus-infec ... | 1988 | 2848209 |
| effects of immunization with bovine herpesvirus-1 glycoproteins on bovine herpesvirus-1-induced alteration of bovine neutrophil chemotactic and anti-pasteurella haemolytica activities. | it has been reported previously that active bovine herpesvirus-1 (bhv-1) infection greatly enhances the susceptibility of cattle to secondary bacterial pneumonia involving pasteurella haemolytica. the present study examines the possibility that immunization of bhv-1 naive calves with purified bhv-1 glycoproteins would protect them against changes in neutrophil function that might compromise their ability to eliminate p. haemolytica during an active bhv-1 infection. the results show that circulat ... | 1988 | 2848379 |
| preincubation of bovine blood neutrophils with bovine herpesvirus-1 does not impair neutrophil interaction with pasteurella haemolytica a1 in vitro. | in this study we examined the direct effects of bovine herpesvirus-1 on the interaction of bovine blood neutrophils with pasteurella haemolytica a1. preincubation of neutrophils for approximately 2 h in vitro with bhv-1 at a multiplicity of infection of 5:1 had no effect on neutrophil random migration and directed migration to zymosan-activated bovine serum. neutrophils also were unimpaired in their ability to ingest and kill p. haemolytica a1. preincubation of neutrophils with bhv-1 did not eli ... | 1988 | 2855289 |
| electron microscopic description of glycocalyx and fimbriae on the surface of pasteurella haemolytica-a1. | several electron microscopic techniques were used to examine the surface of cells of pasteurella haemolytica (biotype a, serotype 1) grown in vitro. all methods showed the presence of a very extensive glycocalyx on logarithmic phase (6 h) cells grown in liquid media. the anionic glycocalyx of these cells stained well with ruthenium red, but collapsed during dehydration for electron microscopy unless stabilized with specific antibodies. when the same techniques were used to examine cells in the s ... | 1987 | 2882831 |
| purification of fimbriae from pasteurella haemolytica a-1. | pasteurella haemolytica a-1 produces large, rigid fimbriae with a diameter of 12 nm and a denisty of 1.32. their subunit molecular weight was 35,000 as determined by sds-polyacrylamide gel electrophoresis and immunoblotting with monoclonal antibodies raised against native fimbriae. the isoelectric point of the purified fimbriae was 4.8. while pasteurella haemolytica whole cells plus a crude shear fraction were capable of agglutinating bovine erythrocytes, purified fimbriae exhibited no such acti ... | 1988 | 2904643 |
| secretion of cyclolysin, the calmodulin-sensitive adenylate cyclase-haemolysin bifunctional protein of bordetella pertussis. | the calmodulin-sensitive adenylate cyclase of bordetella pertussis, a 45 kd secreted protein, is synthesized as a 1706 amino acid precursor. we have shown that this precursor is a bifunctional protein, carrying both adenylate cyclase and haemolytic activities. the 1250 carboxy-terminal amino acids of the precursor showed 25% similarity with escherichia coli alpha-haemolysin (hlya) and 22% similarity with pasteurella haemolytica leucotoxin. three open reading frames were identified downstream fro ... | 1988 | 2905265 |
| cloning, nucleotide sequence, and characterization of genes encoding the secretion function of the pasteurella haemolytica leukotoxin determinant. | the structural gene of the pasteurella haemolytica leukotoxin determinant is highly homologous to that of the escherichia coli hemolysin determinant, which also encodes a specialized set of genes involved in the secretion of the hemolysin. in this report, we describe the cloning and nucleotide sequence of the analogous secretion genes from p. haemolytica which make up the remainder of the leukotoxin determinant. the secretion genes were cloned directly from the p. haemolytica chromosome to form ... | 1989 | 2914876 |
| pasteurella haemolytica antigens associated with resistance to pneumonic pasteurellosis. | antigens associated with whole pasteurella haemolytica biotype a serotype 1, a capsular carbohydrate-protein extract of the organism, and p. haemolytica leukotoxin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. antigens of the electrophoresed preparations were detected by western blotting (immunoblotting) with sera from cattle which were either nonvaccinated or vaccinated with live or killed p. haemolytica vaccines and had variable degrees of resistance to experimen ... | 1989 | 2917783 |
| development and evaluation of an enzyme-linked immunosorbent assay for bovine antibody (igg) to pasteurella haemolytica. | the sensitivity of an indirect enzyme-linked immunosorbent assay (elisa) for bovine igg serum antibody to pasteurella haemolytica was compared with that of an indirect hemagglutination (iha) test. pasteurella haemolytica serotypes were grown in a chemically defined cell culture medium, and soluble antigens released into the growth medium were used in the elisa and iha test. an elisa with serotype-1 antigen consistently detected antibody in sera that were positive by iha test (correlation, 99%). ... | 1989 | 2919816 |
| a retrospective study of hepatic abscesses in goats: pathological and microbiological findings. | post-mortem examination of 658 goats showed that 17 (2.5%) exhibited hepatic abscesses. the following bacteria were isolated: corynebacterium pseudotuberculosis (58.9%), escherichia coli (11.8%), corynebacterium sp. (11.8%), pasteurella haemolytica (5.9%), proteus sp. (5.9%) and staphylococcus aureus (5.9%). hepatic abscesses occurred more frequently in adult animals. eleven of the 17 goats (64.7%) were older than 12 months of age. four goats (23.5%) were 12 months of age and only two (11.8%) we ... | 1989 | 2920279 |
| fatal pasteurella haemolytica pneumonia in bighorn sheep after direct contact with clinically normal domestic sheep. | six rocky mountain bighorn sheep were raised in captivity from birth (n = 5) or taken from the wild as a lamb (n = 1). after the bighorn sheep were in captivity for over a year, 6 clinically normal domestic sheep were placed on the 2 ha of pasture on which the bighorn sheep were kept. nasal swab specimens were obtained from all sheep at the time the domestic sheep were introduced. pasteurella haemolytica was isolated from swab specimens obtained from 4 of 6 domestic sheep, but not from specimens ... | 1989 | 2930021 |
| a rapid micro-method for the study of antibody-mediated killing of bacteria, with specific application to infection of sheep with pasteurella haemolytica. | a micro-titration plate bactericidal assay was developed to measure complement-dependent antibody-mediated killing of pasteurella haemolytica. sera and lung washings from specific pathogen-free (spf) lambs convalescent from a challenge with live, virulent p. haemolytica were bactericidal in the presence of complement. similar samples from naive spf lambs had no such activity. purified igg derived from a convalescent lamb serum was as bactericidal as the whole serum. absorption of convalescent se ... | 1988 | 2967579 |
| effect of intratracheal inoculation of pasteurella haemolytica cytotoxin on the integrity of rat lung. | this study was conducted to investigate the in vivo effect of a single intratracheal inoculation of pasteurella haemolytica cytotoxin on the rat lung. changes in the biochemical and cytological composition of bronchoalveolar lavage fluid were used to estimate the magnitude of pulmonary cell injury, inflammatory response, vascular permeability and functional status of pulmonary alveolar macrophages. effect of treatment was compared with rats intratracheally inoculated with supernatants of pasteur ... | 1987 | 2968834 |
| comparison of the pneumopathogenicity of two strains of bovine viral diarrhea virus. | the pneumopathogenicity in calves of 2 strains of bovine viral diarrhea (bvd) virus, isolate 2724 (a noncytopathogenic virus) and isolate 72 (a cytopathogenic virus), was compared. all calves were inoculated endobronchially, using fiberoptic bronchoscopy. two calves were given pasteurella haemolytica, 2 calves were given the noncytopathogenic bvd virus, and 2 calves were given cytopathogenic bvd virus. five calves were inoculated sequentially with bvd virus and, 5 days later, with p haemolytica. ... | 1985 | 2982295 |
| experimental reproduction of septicemic pasteurellosis in feedlot lambs: bacteriologic and pathologic examinations. | septicemic pasteurellosis (sp) was induced in feedlot lambs. twenty-eight lambs, randomly allotted into 7 groups, were given combinations of 3 treatments: (i) immunosuppression using hydrocortisone solubilized in dimethyl sulfoxide, (ii) rapid changes in feed, from 100% roughage to 90% concentrate, and (iii) oral inoculation of pasteurella haemolytica biotype t. feed changes and immunosuppression by hydrocortisone were needed for the production of sp. pasteurella haemolytica inoculation was not ... | 1985 | 2982297 |
| production of superoxide anion by bovine pulmonary macrophages challenged with soluble and particulate stimuli. | the effects of opsonized zymosan, phorbal myristate acetate, and live pasteurella haemolytica on superoxide anion production by bovine pulmonary macrophages were determined. the anion responses were dose-dependent for all stimuli, except for unopsonized p haemolytica. the effect of viable p haemolytica on macrophage viability was related to bacterial dosage and the presence of opsonizing antibody. superoxide responses varied directly with the dose of opsonized live p haemolytica, but indirectly ... | 1985 | 2986492 |
| effect of bovine alpha 1 interferon on bovine herpesvirus type 1-induced respiratory disease. | treatment of calves with bovine recombinant alpha 1 interferon prior to challenge with bovine herpesvirus type 1 increased the animals' ability to withstand a subsequent pasteurella haemolytica challenge. the reduction in viral-bacterial synergy observed following interferon treatment did not appear to be due to a direct effect of the interferon on virus replication in the upper respiratory tract. thus, even though interferon-treated animals shed slightly less virus from their nasal passages tha ... | 1985 | 2997372 |
| experimentally induced pneumonic pasteurellosis: dose-response relationships and protection against natural reinfection in calves. | calves were inoculated intranasally with 2 x 10(6.2) tissue culture infective doses of infectious bovine rhinotracheitis virus, followed in 7 days by intratracheal inoculations with 1 of 4 challenge doses of pathogenic pasteurella haemolytica. severity and duration of the ensuing clinical signs of respiratory tract disease were correlated with the challenge dose of bacteria. calves given 1 x 10(6) colony-forming units (cfu) of bacteria did not develop reliable clinical evidence of disease, where ... | 1985 | 3002216 |
| bovine herpesvirus-1 and pasteurella haemolytica aerobiology in experimentally infected calves. | eight calves (2 calves in each of 4 groups) were exposed to an aerosol of bovine herpesvirus-1 (bhv-1) and 4 days later to an aerosol of pasteurella haemolytica. samples of tracheal and exhaled air were taken simultaneously beginning 1 day before viral exposure and once a day up to 3 to 4 days after the bacterial exposure. samples were also taken during the period of aerosol exposure. only 0.04% to 0.42% of p haemolytica-carrying droplets of the bacterial aerosol passed beyond the cranial part o ... | 1986 | 3006556 |
| a simple immunological detection method for the direct screening of genes from clone banks. | a simple immunoassay has been developed which can be used in the isolation of particular gene(s) from a clone bank of recombinant plasmids. a clone bank of the dna is constructed with a plasmid vector and maintained in escherichia coli. the recombinant clones were filtered onto a hydrophobic grid membrane and grown up into individual colonies, and a replica was made onto nitrocellulose paper. the bacterial cells were then lysed with chloroform and the proteins were immobilized onto the nitrocell ... | 1986 | 3008788 |
| the effect of parainfluenza virus type 3 on the phagocytic cell response of the ovine lung to pasteurella haemolytica. | groups of caesarean-derived, colostrum-deprived lambs were inoculated by the intratracheal route with pasteurella haemolytica 4 to 6 days after the inoculation of parainfluenza virus type 3 (pi3). some were killed immediately (0 h) and others 24 h later. control groups were inoculated with pi3 alone, p. haemolytica alone or media alone. pulmonary phagocytic cells, p. haemolytica and pi3 were recovered by pulmonary lavage. the phagocytes were separated into alveolar macrophage (am) and neutrophil ... | 1986 | 3010545 |
| cellular inflammatory response in the lungs of calves exposed to bovine viral diarrhea virus, mycoplasma bovis, and pasteurella haemolytica. | three, 5, or 7 days after inoculation with bovine viral diarrhea (bvd) virus (n = 12) or mycoplasma bovis (n = 12), groups of calves were exposed to aerosols of pasteurella haemolytica and were euthanatized 4 hours later. histologic lesions in the lungs and the ratios of neutrophils to alveolar macrophages, collected by bronchoalveolar lavage, were compared with those of clinically healthy calves (n = 8) and calves inoculated with bvd virus only (n = 4), m bovis only (n = 4), or p haemolytica on ... | 1986 | 3014929 |
| induction of immunity against pneumonic pasteurellosis following experimental infection in calves. | immunity against pneumonic pasteurellosis was studied in calves after recovery from experimental respiratory disease with pasteurella haemolytica. nine calves were exposed to aerosols of parainfluenza-3 virus and pasteurella haemolytica a1 six days apart to produce respiratory disease. after recovery from the disease, these nine principal and four control calves were challenged with aerosols of bovine herpesvirus 1 and p. haemolytica a1 four days apart. with this viral-bacterial challenge, the n ... | 1986 | 3017526 |
| the effect of parainfluenza virus type 3 and pasteurella haemolytica on oxygen-dependent and oxygen-independent bactericidal mechanisms of ovine pulmonary phagocytic cells. | groups of caesarean-derived, colostrum-deprived lambs were inoculated by the intratracheal route with pasteurella haemolytica, either alone or 4 or 6 days after the inoculation of parainfluenza virus type 3 (pi3). other groups were inoculated with pi3 followed by veal infusion broth, or with uninfected cell culture fluid followed by veal infusion broth (controls). all lambs were killed 24 h after the second inoculation. pulmonary phagocytic cells were recovered by lavage and separated into alveo ... | 1986 | 3018994 |
| protection of cattle from bovine herpesvirus type i (bhv-1) infection by immunization with individual viral glycoproteins. | the major glycoproteins gi, giii, and giv of bovine herpesvirus-1 (bhv-1) were found to induce high levels of antibody in cattle which could neutralize virus and participate in antibody-dependent cell cytotoxicity of bhv-1-infected cells. immunized animals were fully protected from disease, using a bhv-1/pasteurella haemolytica aerosol challenge model but not from infection with the virus. thus, virus could still replicate in the nasal passages of immunized animals, although to a lesser extent t ... | 1987 | 3037783 |
| use of recombinant bovine alpha 1 interferon in reducing respiratory disease induced by bovine herpesvirus type 1. | intranasal or intramuscular treatment of calves with recombinant bovine alpha 1 interferon before challenge with bovine herpesvirus type 1 and pasteurella haemolytica reduced clinical signs, number of sick days, lung lesions, and weight loss. the effective dose was determined to be relatively broad within the range of 1 to 50 mg per animal. no adverse effects were observed even at high doses of interferon (50 mg per animal). administration before virus infection was more effective than administr ... | 1987 | 3038008 |
| identification of ovine adenovirus types five and six in an epizootic of respiratory tract disease in recently weaned lambs. | eight hundred fourteen polypay-cross lambs, 32 to 35 days old, were housed in an isolation barn under semiconfinement conditions. the lambs were observed twice daily for clinical signs of respiratory tract disease. during the 8-week observation period, an epizootic of respiratory tract disease developed, in which the combined morbidity and mortality was approximately 13%. the overall mortality was 4.1%, of which 57.6% was attributable to pneumonia. pasteurella haemolytica was isolated from 11 (5 ... | 1987 | 3038805 |
| nucleotide sequence of the leukotoxin genes of pasteurella haemolytica a1. | a 4.4-kilobase-pair dna fragment coding for the leukotoxin of pasteurella haemolytica a1 has been isolated, and its nucleotide sequence has been determined. two open reading frames, designated lktc and lkta, coding for proteins of 19.8 and 101.9 kilodaltons, respectively, were identified. expression of the two genes in minicell-labeling experiments resulted in the production of the predicted proteins lktc and lkta. by using an antiserum against the soluble antigens of p. haemolytica a1 in wester ... | 1987 | 3040588 |
| [pathogenesis and immunoprophylaxis of pasteurella haemolytica infections (review)]. | 1988 | 3048241 | |
| bovine pneumonic pasteurellosis: immunity to pasteurella haemolytica. | 1988 | 3060454 | |
| identification of brucella abortus in formalin-fixed, paraffin-embedded tissues of cows, goats, and mice with an avidin-biotin-peroxidase complex immunoenzymatic staining technique. | an avidin-biotin-peroxidase complex immunoenzymatic staining technique was evaluated for light microscopic detection of brucella organisms in formalin-fixed, paraffin-embedded tissues. tissues from cows, goats, and mice inoculated with b abortus strain 2308 were examined, using rabbit antiserum to brucella cell surface protein as primary antibody. stained organisms were identified histologically in tissue sections containing b abortus, as detected by bacteriologic examination of duplicate nonpro ... | 1986 | 3096172 |
| chromatographic separation and characterization of pasteurella haemolytica cytotoxin. | biochemical and immunologic properties of the cytotoxin (leukotoxin) produced by pasteurella haemolytica were examined. crude, bacteria-free supernatants from logarithmic phase p haemolytica were fractionated, using a series of column chromatographic techniques. sequential anion exchange chromatography, gel-filtration chromatography, and chromatofocusing resulted in a cytotoxic substance (cytotoxin-c) of approximately 160 kilodaltons (kd), as determined by use of gel-filtration chromatography. p ... | 1986 | 3096174 |
| bacterial pneumonia stimulates macromolecule secretion and ion and water fluxes in sheep trachea. | in vivo instillation of pasteurella haemolytica (greater than or equal to 10(7) colony-forming units/kg) into a lobar bronchus of sheep produced bacterial pneumonia by 7 days postinoculation. infection was verified bacteriologically and histologically. macromolecule secretion and ion and water fluxes were subsequently measured in tracheal tissues in vitro and were compared with values from sham-infected sheep. macromolecules were radiolabeled with 35so4 and [3h]threonine, and we measured the sec ... | 1987 | 3112107 |
| antibiotic sensitivity of pasteurella haemolytica isolated by means of a fibreoptic endoscope from cases of pneumonic pasteurellosis in cattle. | bacterial isolations from tracheal and bronchial washes obtained with the aid of a fibreoptic endoscope were carried out over a 7 month period in a feedlot on calves suffering from acute pneumonic pasteurellosis. pasteurella haemolytica and pseudomonas aeruginosa represented the majority of isolates. antibiotic sensitivities of the pasteurella isolates are reported on. | 1987 | 3127766 |
| [serotyping of pasteurella haemolytica strains using indirect hemagglutination, double diffusion and countercurrent immunoelectrophoresis]. | 1988 | 3128440 | |
| structure of the o-chain of the lipopolysaccharide of pasteurella haemolytica serotype t3. | cell-wall lipopolysaccharide isolated from pasteurella haemolytica serotype t3 using the phenol-water extraction procedure was shown to be an s type lipopolysaccharide by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. hydrolysis with mild acid afforded a lipid-free, antigenic o-chain polysaccharide. on the basis of one- and two-dimensional 1h and 13c nuclear magnetic resonance studies, in conjunction with microanalytical chemical methods, the o-polysaccharide was determined to be a l ... | 1988 | 3145754 |
| outbreak of pneumonia caused by pasteurella haemolytica infection in shiba goats in japan. | 1988 | 3172608 | |
| experimental contact transmission of pasteurella haemolytica from clinically normal domestic sheep causing pneumonia in rocky mountain bighorn sheep. | two rocky mountain bighorn lambs (ovis canadensis canadensis) were held in captivity for 120 days before being housed with two domestic sheep. the lambs were clinically normal and had no pasteurella spp. on nasal swab cultures. the domestic sheep were known to carry pasteurella haemolytica biotype a in the nasal passages. after being in close contact for 19 days. p. haemolytica biotype a was cultured from nasal swabs of one of the bighorn lambs. by 26 days, both bighorn sheep developed coughs, w ... | 1988 | 3193561 |
| haemolytic effect of pasteurella haemolytica on blood from young mammals. | on agar plates containing young lamb blood, pasteurella haemolytica produces a wide outer zone of partial haemolysis in addition to the narrow zone of complete clearing seen on adult sheep blood agar. to determine whether this phenomenon was limited to lamb blood, samples from young animals of 20 mammalian species were examined. two species--the barbary sheep (ammotragus lervia) and scimitar horned oryx (oryx tao)--possessed blood that gave this effect provided that the samples were taken from y ... | 1988 | 3194597 |
| susceptibility of rocky mountain bighorn sheep and domestic sheep to pneumonia induced by bighorn and domestic livestock strains of pasteurella haemolytica. | bighorn sheep were inoculated intratracheally with suspensions of nonhemolytic pasteurella haemolytica biotype t (10(12) organisms) unique to wild bighorns, with beta-hemolytic p. haemolytica biotype t (10(12) organisms) isolated from clinically normal domestic sheep or intradermally with half a dose of a cattle vaccine containing p. haemolytica biotype a (10(5) organisms). the bighorn strain caused lobar necrotizing bronchopneumonia whereas both domestic livestock strains precipitated fatal sep ... | 1988 | 3196974 |
| elisa for the measurement of sheep antibodies to the capsular antigens of pasteurella haemolytica serotypes. | an enzyme-linked immunosorbent assay utilising direct binding of capsular polysaccharide antigens to polystyrene immunoassay plates was used to measure sheep antibodies to pasteurella haemolytica a1, a2 and a6 serotypes. low level cross reactivity occurred between a2 antigen and heterologous antisera. a strong unilateral cross reaction between a1 antigen and anti-a6 serum was abolished by absorption. these reactions suggest shared capsular antigens between serotypes. | 1988 | 3212293 |
| genetic determinant of the rob-1 beta-lactamase in bovine and porcine pasteurella strains. | the rob-1 beta-lactamase, previously described in haemophilus influenzae, has been found in the genus pasteurella. in three bovine strains of pasteurella multocida and pasteurella haemolytica, rob-1 production was determined by plasmids of 4.4 kilobases. in one porcine strain of pasteurella aerogenes, the enzyme seems to be chromosomally encoded. | 1988 | 3263836 |
| activation of protein breakdown and prostaglandin e2 production in rat skeletal muscle in fever is signaled by a macrophage product distinct from interleukin 1 or other known monokines. | during sepsis or after injection of endotoxin into rats, there is a large increase in muscle protein breakdown and prostaglandin e2 (peg2) production. prior studies showed that partially purified interleukin 1 (il-1) from human monocytes can stimulate these processes when added to isolated rat muscles. the availability of pure recombinant il-1 and other monokines has allowed us to investigate the identity of the active agent in this process. incubation of muscles with recombinant human or murine ... | 1988 | 3284911 |
| efficacy of a streptomycin-dependent, live pasteurella haemolytica vaccine against challenge exposure to pasteurella haemolytica in cattle. | a streptomycin-dependent, live pasteurella haemolytica vaccine was given in 1 or 2 doses to 2 groups of weaned calves; 2 other groups of calves were not vaccinated. all calves in the vaccinated groups and calves in 1 of the nonvaccinated groups were stressed by transport, intratracheally inoculated with bovine herpesvirus type-1 (cooper strain), and then intratracheally inoculated with p haemolytica type a1. the 4th group of calves (nonvaccinated controls) was not stressed and were not intratrac ... | 1987 | 3296883 |
| analysis of major antigens of haemophilus (actinobacillus) pleuropneumoniae and related organisms. | outer membrane protein (omp)-enriched extracts and whole-cell protein preparations of haemophilus (actinobacillus) pleuropneumoniae and related organisms were examined by polyacrylamide gel electrophoresis and immunoblotting. both the omp-enriched and whole-cell protein profiles of actinobacillus suis, a. pleuropneumoniae (nad-independent biovar), a. lignieresii, and pasteurella haemolytica were very similar to those of h. pleuropneumoniae serotypes 1 to 8. antisera prepared against h. pleuropne ... | 1987 | 3298061 |
| identification and characterization of the pasteurella haemolytica leukotoxin. | the identification and chromatographic characterization of the leukotoxin of pasteurella haemolytica is described. the toxin, which has an apparent native molecular weight of greater than 400,000 as judged by gel exclusion chromatography, has a 105-kilodalton (105k) polypeptide as its major protein component. the proteolytic degradation of the 105k polypeptide could be correlated with the loss of toxin activity in aging cultures of p. haemolytica. antisera raised against purified 105k polypeptid ... | 1987 | 3308703 |
| immunoperoxidase evaluation of pneumonic lesions induced by pasteurella haemolytica in calves. | calves inoculated with pasteurella haemolytica serovar 1 developed lesions of coagulation necrosis in the lungs that were sharply demarcated by leukocytes. the p haemolytica antigen was detected in the area of coagulation necrosis in histologic sections, using an immunoperoxidase technique. in the central area of the necrotic tissue, the bacterial antigen was diffusely presented in the necrotic alveolar wall, fibrin, serous exudate, and degenerated leukocyte. the bacterial antigen also was found ... | 1987 | 3310766 |
| failure of a pasteurella haemolytica extract vaccine to protect calves against experimental pneumonic pasteurellosis. | 1987 | 3314101 | |
| extensive homology between the leukotoxin of pasteurella haemolytica a1 and the alpha-hemolysin of escherichia coli. | the 19.8- and 101.9-kilodalton leukotoxin proteins of pasteurella haemolytica (lktc and lkta, respectively) share extensive homology with the hlyc and hlya alpha-hemolysin proteins of escherichia coli. the leukotoxin lkta protein cross-reacts with hemolysin-specific antisera in western blot (immunoblot) analysis, indicating that it shares epitopes with the alpha-hemolysin hlya protein. both lkta and hlya contain a conserved hydrophobic region, as well as a set of tandemly repeated domains. these ... | 1987 | 3316038 |
| vaccination of calves with leukotoxic culture supernatant from pasteurella haemolytica. | in three experiments subcutaneous vaccination of calves with adjuvanted bacteria-free leukotoxic culture supernatant from log phase cultures of pasteurella haemolytica a1 (toxin 1) was shown to induce some protection against intrabronchial challenge with live p. haemolytica a1. this toxin 1 vaccine was as effective as a whole cell bacterin in stimulating agglutinating antibody to p. haemolytica. induction of leukotoxin neutralizing activity was variable; in some cases vaccination only primed the ... | 1988 | 3349399 |
| acute mastitis and disseminated intravascular coagulopathy caused by pasteurella haemolytica in a cow. | pasteurella haemolytica was found to be the cause of acute mastitis, toxemia, and disseminated intravascular coagulopathy in a cow. intensive treatment with antibiotics and fluid and heparin administration failed to reverse the progression of the disease, and death resulted. necropsy revealed extensive evidence of consumptive coagulopathy, as well as mastitis. pasteurella haemolytica rarely has been implicated as a cause of mastitis in cows. | 1988 | 3350743 |
| development and evaluation of an enzyme-linked immunosorbent assay for bovine antibody to pasteurella haemolytica: constructing an enzyme-linked immunosorbent assay titer. | a 2-stage strategy was developed and evaluated for estimating serum antibody titer by use of elisa and a series of dilutions. in stage 1, the linear response region and least-square estimate of the assay line slope were established from 9-point dilution assays. provided that the reading was within the linear response region, this information was used in the stage-2 estimation of titer from a single absorbance reading. operationally, 2 fixed dilutions were selected, one suitably low and one suita ... | 1988 | 3354965 |
| [detection of antibodies to pasteurella multocida and pasteurella haemolytica in the serum of calves]. | 1988 | 3369910 | |
| pulmonary lesions induced by pasteurella haemolytica in neutrophil sufficient and neutrophil deficient calves. | the role of neutrophils in the development of peracute lung lesions of bovine pneumonic pasteurellosis was investigated. eight calves were divided into two groups of four calves each. group i was treated with intravenous phosphate-buffered saline and served as the neutrophil sufficient calves. group ii was treated with intravenous hydroxyurea which produced a state of neutropenia. when peripheral blood neutrophil numbers dropped below 300 cells/microl in group ii, all calves were challenged with ... | 1988 | 3370555 |
| growth of an acanthamoeba isolate on a gram-negative bacterium, probably pasteurella haemolytica. | a gram-negative bacterium, probably pasteurella haemolytica, was found to support the growth of acanthamoeba spp. this provides a useful means for initial isolation of acanthamoeba spp. and for culturing these parasites to high cell densities. | 1988 | 3379534 |
| infectious agents in respiratory disease of housed, fattening lambs in northern ireland. | a two-year longitudinal, microbiological and pathological survey of respiratory disease in lambs housed for fattening at three-and-a-half to four months of age was undertaken. in the first year samples of nasal mucus and blood were taken from lambs each week for the first nine weeks after entry to a fattening unit and each week one lamb was examined post mortem. in the second year two additional fattening units were included in the survey, when samples of blood and nasal mucus were taken from la ... | 1988 | 3381463 |
| comparison of the toxic and antigenic properties of single bovine isolates of pasteurella haemolytica representing five serotypes and an untypable strain. | single strains of 5 different p. haemolytica serotypes (1, 2, 5, 6 and 9) and an untypable strain were compared in an attempt to detect differences which might be related to virulence. all but the untypable strain caused extensive lesions when injected into the lungs of healthy cattle. each strain was found to be encapsulated and to be toxic in vitro for bovine leukocytes. each strain also produced leukotoxin in vitro. the toxins varied, however, in total toxic activity and in the kinetics of le ... | 1988 | 3381490 |
| characterisation of a new serotype of p haemolytica isolated in hungary. | three strains belonging to a new serotype of pasteurella haemolytica (a16) were isolated from lambs and a wild boar in hungary. the identity and validity of the new serotype was proved by biochemical tests and by the indirect haemagglutination test using unabsorbed and absorbed hyperimmune sera raised in rabbits. | 1988 | 3406538 |
| effects of environmental temperature, relative humidity and vaccination on pasteurella haemolytica in lungs of mice. | groups of mice were kept in four combinations of environmental temperature (8 and 20 degrees c) and humidity [50 per cent and 90 per cent relative humidity (rh)]. the mice were anaesthetized and inoculated intranasally with pasteurella haemolytica growing logarithmically. groups of 11 mice were killed at 0.5 h intervals for 6 h after inoculation and the number of colony-forming-units (cfu) of pasteurella haemolytica in the lungs was determined. the cfu in the lungs of mice were affected by a sig ... | 1988 | 3417911 |
| plasmids and resistance to 9 chemotherapeutic agents of pasteurella multocida and pasteurella haemolytica. epidemiological aspects. | 1987 | 3434027 | |
| adaptation of a colorimetric microtitration assay for quantifying pasteurella haemolytica a1 leukotoxin and antileukotoxin. | a colorimetric microtitration assay was adapted to quantify the cytotoxicity of pasteurella haemolytica a1 leukotoxin to bovine neutrophils used as target cells. the viability of leukotoxin-treated target cells was detected by use of a tetrazolium dye that living cells reduced to dark blue formazan. the amount of formazan formed (which was quantified by use of an elisa plate reader) was directly proportional to the number of viable target cells. this assay system also was used to measure leukoto ... | 1987 | 3434898 |
| induced pasteurellosis and pulmonary vascular adrenoceptor function in bull calves. | effects of pasteurella haemolytica inoculation on pulmonary vascular function were studied in 5 conscious standing jersey bull calves. instruments were implanted in each calf to measure pulmonary arterial, pulmonary arterial wedge, left atrial, and systemic arterial pressures and cardiac output. each calf was challenge exposed with 5 sequential 3-minute infusions of isoproterenol (a beta agonist) or phenylephrine (an alpha agonist) for maximal doses of 1.8 micrograms of isoproterenol or 2.3 micr ... | 1987 | 3434901 |
| pulmonary dysfunction in neonatal calves after intratracheal inoculation of small volumes of fluid. | intratracheal instillation of 20 ml of room temperature (21 to 24 c) fluid in anesthetized neonatal calves resulted in rapid onset of reversible pulmonary dysfunction. arterial o2 tension and dynamic compliance decreased, whereas pulmonary arterial pressure, pulmonary vascular resistance, alveolar arterial o2 difference, and total pulmonary resistance increased from base-line values. abnormalities of gas exchange and pulmonary mechanics were induced by intratracheal fluid instillation whether or ... | 1987 | 3434902 |
| efficacy of sulbactam-ampicillin in an induced pasteurella haemolytica pneumonia model in calves. | therapeutic efficacy of sulbactam, a beta-lactamase inhibitor, in combination with ampicillin was evaluated in an ampicillin-resistant pasteurella haemolytica pneumonia model in cattle, using an iv agar emboli method of infection. groups of cattle given vehicle (group 1, n = 19) or ampicillin (group 2, n = 8) had 74% and 50% mortality, respectively, whereas group 3 (n = 11) given sulbactam-ampicillin had no mortality. morbidities were 100% in groups 1 and 2 and 27% in group 3. retrospectively, m ... | 1987 | 3434913 |
| [serotypes of pasteurella haemolytica in lungs with pneumonia from sheep in mexico]. | 1987 | 3438607 | |
| purulent epididymitis in a sucking lamb caused by pasteurella haemolytica. | 1987 | 3447464 | |
| automated colorimetric assay for the detection of pasteurella haemolytica leucotoxin. | a rapid and easily performed colorimetric method has been adapted for the detection of the pasteurella haemolytica leucotoxin using the lysis of bovine leucocyte bl-3 cells (a bovine lymphoid cell line obtained from dr. g. theilen, university of california, davis). the method involves incubating bl-3 cells in the presence of p. haemolytica culture supernatant and assessing cell survival at the end of the assay by staining remaining bl-3 cells with the dye neutral red. solubilization of the cells ... | 1986 | 3487878 |
| a live pasteurella haemolytica vaccine efficacy trial. | a live pasteurella haemolytica serotype 1 vaccine was used in an efficacy trial conducted on 100 lightweight feeder calves purchased from a florida ranch. forty-one calves were inoculated with the vaccine intradermally in the neck. fifty-nine calves served as nonvaccinated controls. fourteen days later, the calves were shipped to an order buyer in eastern tennessee, where the calves were mixed with 60 local calves in a community sale barn for 72 hours. after 3 additional days, the calves were sh ... | 1986 | 3514562 |
| hemodynamic effects of acute pneumonia experimentally induced in newborn calves inoculated with pasteurella haemolytica. | hemodynamic responses to acute pneumonia and to hypoxia were investigated in 10 newborn calves. experiments were performed on heparinized, anesthesized and ventilated calves. control calves were inoculated intratracheally with bovine fetal serum. pneumonia was induced in treated calves by intratracheal inoculation with p haemolytica suspended in bovine fetal serum. before inoculation (base line), at the time of inoculation (t = 0), and at 30-minute intervals for 3 hours, pulmonary arterial press ... | 1986 | 3524330 |
| cloning of a serotype-specific antigen from pasteurella haemolytica a1. | recombinant plasmids coding for a soluble (or surface) antigen of pasteurella haemolytica a1 were identified. two plasmids, both containing the same 5.4 kilobase pairs of insert dna, were recovered independently by screening a clone band of p. haemolytica a1 genomic dna in escherichia coli for the expression of p. haemolytica a1 soluble antigens (r. y. c. lo and l.a. cameron, can. j. biochem. cell biol. 64:73-76, 1986). e. coli cells carrying the plasmids were found to be agglutinated by an anti ... | 1986 | 3527985 |
| immunologic response and resistance to experimentally induced pneumonic pasteurellosis in cattle vaccinated with various dosages of lyophilized pasteurella haemolytica. | pasteurella haemolytica was lyophilized in an enriched soybean polypeptone broth. lyophilization in this medium resulted in a mean 10-fold loss in p haemolytica viability, as opposed to up to a 10(4)-fold loss in viability when other media were used. lyophilized p haemolytica was reconstituted and used as a live vaccine in 3 experiments. calves were challenge exposed by transthoracic injection with virulent p haemolytica. in experiment 1, 2 subcutaneous injections (7-day interval between injecti ... | 1986 | 3530067 |
| an evaluation of the api zym system as a means of identifying haemophilus somnus and related taxa. | the commercially available api zym microbiological identification system was evaluated for the rapid identification of haemophilus somnus. eighty-seven isolates of the organism had api zym profiles which were characteristic. the api zym profiles demonstrate clear differences between h. somnus and other genera but suggest a close association to three related organisms. enzyme activity of h. somnus isolates were similar to organisms identified as histophilus ovis, haemophilus agni and strains uqv ... | 1986 | 3530416 |
| assessment of immunity to pasteurella haemolytica in sheep by in vitro methods. | the sera of conventionally-reared sheep which had been experimentally infected with pasteurella haemolytica serotype a2 and mycoplasma ovipneumoniae were compared with those of animals from the same source which had been vaccinated 3 times with an alhydrogel-adsorbed mixture of cell-wall extract and heat-killed cells of p. haemolytica a2 emulsified in freund's incomplete adjuvant. both groups of animals had high titres of anti-p. haemolytica a2 igg by elisa, high opsonic indices in a phagocytic ... | 1986 | 3539670 |
| some aspects of the occurrence of resistant bacteria in the normal animal flora. | the paper outlines some of the problems encountered in assessing the occurrence of antibiotic resistance in the normal flora of animals, using escherichia coli as an example. additional information is provided on the occurrence and mechanisms of resistance in clostridium perfringens, pasteurella haemolytica and staphylococcus aureus, and some of the factors which may affect this resistance. the paper concludes with general considerations about the choice of organism, the sample size and the desi ... | 1986 | 3542933 |
| pneumonic pasteurellosis: examination of typable and untypable pasteurella haemolytica strains for leukotoxin production, plasmid content, and antimicrobial susceptibility. | plasmid dna screening experiments were conducted to determine whether a relationship existed between the presence of plasmids and antibiotic resistance in pasteurella haemolytica or the capability to produce hemolysin or leukotoxin (cytotoxin). regardless of plasmid content, all p haemolytica isolates produced characteristic hemolysis on blood agar plates. similarly, standardized suspensions of living bacteria and sterile concentrated (approx 200:1) culture supernatant from strains representing ... | 1987 | 3565892 |
| antigenic specificity of convalescent serum from cattle with haemophilus somnus-induced experimental abortion. | the antigens of haemophilus somnus recognized by convalescent bovine serum were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting with a protein a-peroxidase conjugate. the same two 76k and 40k antigens were predominant in whole-bacterium preparations and in outer-membrane-enriched, triton x-100-insoluble fractions. the surface location of these two antigens was confirmed by absorbing antiserum with whole, live bacteria. absorption with h. somnus removed a ... | 1987 | 3570470 |
| in vitro antibacterial properties of el-870, a new semi-synthetic macrolide antibiotic. | a new macrolide antibiotic, el-870, 20-deoxo-20-(3,5-dimethylpiperidin-1-yl)desmycosin, has been prepared by chemical modification of desmycosin. in vitro, against selected animal bacterial pathogens, it inhibited growth of pasteurella multocida, pasteurella haemolytica, mycoplasma hyopneumoniae, actinobacillus pleuropneumoniae, streptococcus suis, actinomyces pyogenes and certain other bacteria at levels of 6.25 micrograms/ml or less. in general, the mics for gram-negative enteric bacteria have ... | 1987 | 3570967 |
| proliferation of pasteurella haemolytica in the calf respiratory tract after an abrupt change in climate. | two groups of four calves were exposed to a poly-disperse aerosol of 1.3 x 10(4) to 16 x 10(4) colony forming units (cfu) of nalidixic acid resistant pasteurella haemolytica type a1 litre-1 of air with a mass median aerodynamic diameter of 2.6 +/- 0.8 microns. one group of calves was kept at 5 degrees c, 72 per cent relative humidity (rh) and the second was subjected to an abrupt change in climate directly after aerosol exposure from 5 degrees c, 75 per cent rh to 13 degrees c, 84 per cent rh. c ... | 1987 | 3589165 |
| pasteurella haemolytica cytotoxin: relative susceptibility of bovine leucocytes. | an in vitro 51cr-release assay was used to compare the susceptibility of various leucocytes from normal cattle to pasteurella haemolytica cytotoxin. neutrophils were found to be more sensitive than mammary or bronchoalveolar macrophages. neutrophils induced with lipopolysaccharide (lps) and mammary macrophages activated in vitro with lps were as sensitive as homologous untreated cells. bronchoalveolar macrophages from adult cows were significantly more resistant than those from calves. sub-cytol ... | 1987 | 3604053 |
| ingestion and killing of pasteurella haemolytica a1 by bovine neutrophils in vitro. | in this study, various parameters affecting the ability of bovine neutrophils to ingest and kill a virulent strain of pasteurella haemolytica a1 in vitro were examined. ingestion of p. haemolytica was serum dependent (optimal serum concentration 10%) and was mediated principally by heat-stable opsonins, presumably antibodies, that could be removed by absorption with formalin-killed p. haemolytica. ingested p. haemolytica were killed by neutrophils within 1-4 h incubation; the magnitude of killin ... | 1987 | 3629916 |
| the immune response to experimental pasteurella haemolytica infection in calves. | four male dairy calves, ages 1-9 months, were inoculated intratracheally (it), with log dilutions (1.5 x 10(3)-1.5 x 10(6)) of an isolate of p. haemolytica a-1. doses of bacteria varied according to ages of the calves, older calves receiving the larger doses. all four calves became severely ill within 24 h after inoculation and antibiotic treatment was considered essential. two months later the four calves remained healthy after it injection of p. haemolytica, again given in log dilution (2.8 x ... | 1987 | 3629917 |
| ceftiofur sodium, a broad-spectrum cephalosporin: evaluation in vitro and in vivo in mice. | ceftiofur sodium, a broad-spectrum beta-lactamase-resistant cephalosporin, was evaluated in vitro and in vivo in mice. ceftiofur is the sodium salt of (6r, 7r)-7[( 2-amino-4-thiazolyl)-z- (methoxyimino)acetyl]amino)-3-[( (2-furanylcarbonyl)thio]methyl)-8-oxo-5- thia-1-azabicyclo-[4.2.0]oct-2-ene-2-carboxylate. minimal inhibitory concentration values were obtained with 264 strains representing 9 genera and 17 species of bacterial pathogens from cattle, swine, sheep, horses, poultry, dogs, cats, a ... | 1987 | 3631686 |
| [results of experimental infection of calves with pasteurella multocida and pasteurella haemolytica]. | 1987 | 3632244 | |
| new serotype of pasteurella haemolytica isolated in hungary. | 1987 | 3660552 | |
| vaccines for respiratory disease in cattle. | respiratory disease is one of the most serious disease complexes affecting beef cattle production. for example, it is claimed to cost the uk industry about 70 million pounds per year. it is usually associated with young cattle and can occur in a variety of situations. it is a good example of multifactorial disease in that its aetiology involves both infection by a variety of microorganisms and a number of environmental factors. several distinct syndromes occur and a number of microorganisms are ... | 1987 | 3673201 |
| neutralization of pasteurella haemolytica leucotoxin by bovine immunoglobulins. | 1987 | 3676692 | |
| the efficacy of sulbactam-ampicillin in the therapy of respiratory disease associated with ampicillin resistant pasteurella species in housed calves. | sulbactam-ampicillin is a combination of sulbactam, a beta-lactamase inhibitor, and ampicillin, a broad spectrum beta-lactam antibiotic. the efficacy of sulbactam-ampicillin was evaluated in the treatment of calf respiratory disease associated with ampicillin-sensitive and ampicillin-resistant strains of pasteurella haemolytica and pasteurella multocida. treatment with sulbactam-ampicillin was compared with treatment with ampicillin alone in 123 friesian calves, between three and five weeks old, ... | 1987 | 3686802 |
| stress diminishes infiltration and oxygen metabolism of phagocytic cells in calves. | the influences of a stress situation on the phagocytic cell function before and after infection with pasteurella haemolytica were measured in calves. no differences in phagocytic and metabolic activity of alveolar macrophages (ams) were observed in vitro between cells isolated from stressed and nonstressed animals. the uptake of bacteria and the migration of polymorphonuclear leukocytes (pmns) did not differ. however, the production of superoxide by pmns isolated from stressed animals was signif ... | 1987 | 3692578 |
| comparison of single strains of four serotypes of pasteurella haemolytica biotype a in experimental pneumonia of sheep. | single strains of serotypes a1, a2, a7 and a9 of pasteurella haemolytica were separately used in combination with mycoplasma ovipneumoniae to reproduce pneumonia. macroscopically and microscopically the pneumonias associated with individual serotypes were similar and it is concluded that serotypes of p haemolytica isolated with low frequency in field disease may be equally virulent to common serotypes. | 1986 | 3704319 |
| attempt to prevent the effects of experimental chronic pneumonia in sheep by vaccination against pasteurella haemolytica. | 1986 | 3708312 | |
| serum antibodies to pasteurella haemolytica lipopolysaccharide: relationship to experimental bovine pneumonic pasteurellosis. | an enzyme-linked immunosorbent assay was used to determine the serum antibody response to pasteurella haemolytica lipopolysaccharide (lps) for calves vaccinated with saline solution, a formalin-killed p haemolytica bacterin, or live p haemolytica. bacterin-vaccinated calves had a lower antibody response to lps than did calves vaccinated with live p haemolytica. calves vaccinated with either saline solution or the bacterin were more susceptible to intrapulmonic challenge exposure with p haemolyti ... | 1986 | 3717738 |