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insecticidal activity of ethyl acetate extracts from culture filtrates of mangrove fungal endophytes.in the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude ethyl acetate culture filtrate extracts from 110 mangrove fungal endophytes were screened for their toxicity. toxicity tests of all extracts against brine shrimp (artemia salina) larvae were performed. the extracts with the highest toxicity were further examined for insecticidal activity against spodoptera litura larvae and acetylcholinesterase (ache) inhibition activity. the results showed th ...201526190921
[characterization and cloning of the cry2a gene from indigenous isolates of bacillus thuringiensis].discovery of novel cry genes of bacillus thuringiensis (bt) with higher toxicity is important for the development of transgenic bt crops resistant to target pests. two new indigenous isolates of bt were characterized for their colony type, crystal inclusion and toxicity with helicoverpa armigera hubner and spodoptera litura linn. screening of cry2a genes from the two new isolates of bt, t30 and t48, by pcr with cry2a family primers showed the presence of cry2a genes only in the isolate t30. furt ...201726299858
identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm spodoptera litura.insect odorant binding proteins (obps) are thought to involve in insects' olfaction perception. in the present study, we identified 38 obp genes from the antennal transcriptomes of spodoptera litura. tissue expression profiles analysis revealed that 17 of the 38 slitobp transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. the rpkm value analysis revealed that seven obps (slitpbp1-3, slitgobp1-2, slitobp3 and slitobp5) a ...201526346731
growth performance and biometric characteristics of spodoptera litura (lepidoptera: noctuidae) reared on different host plants.spodoptera litura (f.), an important polyphagous insect pest, attacks ca. 300 economic crops in dozens of countries. investigations into its growth and development performance on different host plants can provide an understanding of the potential for increase of s. litura population in the field. we measured the development time, body weight, and head capsule width of s. litura larvae reared on cabbage, taro, peanut, and sesbania, a green manure. larvae reared on cabbage ingested a significantly ...201526453712
the chitinase c gene pschic from pseudomonas sp. and its synergistic effects on larvicidal activity.pseudomonas sp. strain txg6-1, a chitinolytic gram-negative bacterium, was isolated from a vegetable field in taixing city, jiangsu province, china. in this study, a pseudomonas chitinase c gene (pschic) was isolated from the chromosomal dna of this bacterium using a pair of specific primers. the pschic gene consisted of an open reading frame of 1443 nucleotides and encoded 480 amino acid residues with a calculated molecular mass of 51.66 kda. the deduced pschic amino acid sequence lacked a sign ...201526500441
ectopic overexpression of wssgtl1, a sterol glucosyltransferase gene in withania somnifera, promotes growth, enhances glycowithanolide and provides tolerance to abiotic and biotic stresses.overexpression of sterol glycosyltransferase (sgtl1) gene of withania somnifera showing its involvement in glycosylation of withanolide that leads to enhanced growth and tolerance to biotic and abiotic stresses. withania somnifera is widely used in ayurvedic medicines for over 3000 years due to its therapeutic properties. it contains a variety of glycosylated steroids called withanosides that possess neuroregenerative, adaptogenic, anticonvulsant, immunomodulatory and antioxidant activities. the ...201626518426
efficacy of sub lethal concentration of entomopathogenic fungi on the feeding and reproduction of spodoptera litura.in the present investigation, impact of sub lethal concentrations of entomopathogenic fungi, namely isaria fumosorosea, beauveria bassiana and paecilomyces variotii, secondary metabolite on feeding, growth, fecundity and hatchability of spodoptera litura was performed. the s. litura treated with i. fumosorosea and b. bassiana metabolites exhibited renounced food consumption. the growth rate of treated s. litura with metabolite of i. fumosorosea had drastic reduction. in the case of approximate d ...201526558183
demography and consumption of spodoptera litura (lepidoptera: noctuidae) reared on cabbage and taro.spodoptera litura (f.) causes considerable economic damage to multiple agro-crops annually in many countries. in this study, the demography of s. litura reared on cabbage and taro was investigated using the age-stage, two-sex life table at 25±1°c, 60±10% relative humidity, and a photoperiod of 12: 12 (l:d) h. our results showed that the net reproductive rate, intrinsic rate, and finite rate of population increase on cabbage (1893.1 offspring, 0.2374 d(−1), and 1.2679 d(−1)) were all not signific ...201626578628
evidence of recent interspecies horizontal gene transfer regarding nucleopolyhedrovirus infection of spodoptera frugiperda.baculoviruses are insect-associated viruses carrying large, circular double-stranded-dna genomes with significant biotechnological applications such as biological pest control, recombinant protein production, gene delivery in mammals and as a model of dna genome evolution. these pathogens infect insects from the orders lepidoptera, hymenoptera and diptera, and have high species diversity which is expressed in their diverse biological properties including morphology, virulence or pathogenicity. s ...201526607569
conversion of volatile alcohols into their glucosides in arabidopsis.exposure of tomato plants to volatile chemicals emitted from common cutworm (spodoptera litura)-infested conspecifics led to accumulation of the glycoside, (z)-3-hexenyl vicianoside. accumulation of (z)-3-hexenyl vicianoside in the exposed plants has adverse impacts on the performance of the common cutworms. the aglycon of (z)-3-hexenyl vicianoside is derived from airborne (z)-3-hexenol emitted from infested plants. the ability to incorporate and convert (z)-3-hexenol to its corresponding glycos ...201726629260
glutathione s-transferase slgste1 in spodoptera litura may be associated with feeding adaptation of host plants.spodoptera litura is polyphagous pest insect and feeds on plants of more than 90 families. in this study the role of glutathione s-transferase epilson 1 (slgste1) in s. litura in detoxification was examined. this gene was up-regulated in the midgut of s. litura at the transcriptional and protein levels when the insect fed on brassica juncea or diet containing phytochemicals such as indole-3-carbinol and allyl-isothiocyanate that are metabolic products of sinigrin and glucobrassicin in b. juncea. ...201626631599
in-silico determination of insecticidal potential of vip3aa-cry1ac fusion protein against lepidopteran targets using molecular docking.study and research of bt (bacillus thuringiensis) transgenic plants have opened new ways to combat insect pests. over the decades, however, insect pests, especially the lepidopteran, have developed tolerance against bt delta-endotoxins. such issues can be addressed through the development of novel toxins with greater toxicity and affinity against a broad range of insect receptors. in this computational study, functional domains of bacillus thuringiensis crystal delta-endotoxin (cry1ac) insectici ...201526697037
physiological effects of resveratrol and coumaric acid on two major groundnut pests and their egg parasitoid behavior.groundnut, arachis hypogea l., is one of the plant species that synthesizes phenolic compounds, resveratrol and coumaric acid. they are induced as a defense strategy in plant in response to feeding lepidopterans. the present study investigated the role of resveratrol and coumaric acid in producing antiherbivore effects as a direct defense against two major groundnut pests, spodoptera litura f. and amsacta albistriga w., and in indirect defense by attracting the egg parasitoid trichogramma chilon ...201626890503
cloning, characterization and expression of a novel haplotype cry2a-type gene from bacillus thuringiensis strain swk1, native to himalayan valley kashmir.bacillus thuringiensis (bt) is a gram positive bacterium which is effectively being used in pest management strategies as an eco-friendly bioinsecticide. in the present study a new cry2a gene was cloned from a promising indigenous b. thuringiensis swk1 strain previously characterized for its toxicity against spodoptera litura and helicoverpa armigera larvae. the nucleotide sequence of the cloned cry2a gene pointed out that the open reading frame has 1902 bases encoding a polypeptide of 634 amino ...201626906447
expression and purification of virus like particles (vlps) of foot-and-mouth disease virus in eri silkworm (samia cynthia ricini) larvae.foot-and-mouth disease (fmd) is a highly contagious viral disease, which causes severe economic loss to livestock. virus like particles (vlps) produced by recombinant dna technology are gaining importance because of their immunogenic properties and safety in developing a new vaccine for fmd. in the present study, a practical and economically feasible approach of expression, purification and characterization of vlps of fmdv in eri silkworm (samia cynthia ricini) larvae was described. although thr ...201626925448
herbicidal and cytotoxic constituents from aralia armata (wall.) seem.two new triterpenoids, 3β-hydroxyoleana-11,13(18)-diene-28,30-dioic acid (1) and 3-oxooleana-11,13(18)-diene-28,30-dioic acid (2), one novel triterpenoid glycoside, 3β-o-(6'-o-methyl-β-d-glucuronopyranosyl)oleana-11,13(18)-dien-28-oic acid (3) along with six known compounds (4 - 9) were isolated from the stem bark of aralia armata (wall.) seem. their structures were elucidated through extensive spectroscopic methods. the herbicidal activities of these compounds against bidens pilosa l., an invas ...201626948515
molecular and in silico characterization of achaea janata granulovirus granulin gene.achaea janata granulovirus (acjagv), an insect virus belonging to baculoviridae, infects semilooper, a widely distributed defoliating pest on castor beans (ricinus communis l.) and several other plant hosts in india. the propagation and purification of the hyderabad isolate acjagv were performed, granulin gene from this isolate was amplified, cloned and sequenced, and its homology with other known granulin genes was assessed. the 753-bp granulin orf of acjagv encoded for a granulin protein of 25 ...201626984814
sweet potato nac transcription factor, ibnac1, upregulates sporamin gene expression by binding the swre motif against mechanical wounding and herbivore attack.sporamin is a tuberous storage protein with trypsin inhibitory activity in sweet potato (ipomoea batatas lam.), which accounts for 85% of the soluble protein in tubers. it is constitutively expressed in tuberous roots but is expressed in leaves only after wounding. thus far, its wound-inducible signal transduction mechanisms remain unclear. in the present work, a 53-bp dna region, sporamin wound-response cis-element (swre), was identified in the sporamin promoter and was determined to be respons ...201626996980
transcriptional profiling analysis of spodoptera litura larvae challenged with vip3aa toxin and possible involvement of trypsin in the toxin activation.vip proteins, a new group of insecticidal toxins produced by bacillus thuringiensis, are effective against specific pests including spodoptera litura. here, we report construction of a transcriptome database of s. litura by de novo assembly along with detection of the transcriptional response of s. litura larvae to vip3aa toxin. in total, 56,498 unigenes with an n50 value of 1,853 bp were obtained. results of transcriptome abundance showed that vip3aa toxin provoked a wide transcriptional respon ...201627025647
molecular characterization of autophagy-related gene 5 from spodoptera exigua and expression analysis under various stress conditions.autophagy is not only involved in development, but also has been proved to attend immune response against invading pathogens. autophagy protein 5 (atg5) is an important autophagic protein, which plays a crucial role in autophagosome elongation. although atg5 has been well studied in mammal, yeast, and drosophila, little is known about atg5 in lepidopteran insects. we cloned putative seatg5 gene from spodoptera exigua larvae by the rapid amplification of cdna ends method, and its characteristics ...201627226059
insecticidal potential of an endophytic cladosporium velox against spodoptera litura mediated through inhibition of alpha glycosidases.alpha glucosidase inhibitory activity was exhibited by partially purified fractions obtained from an endophytic cladosporium velox, isolated from tinospora cordifolia. taking into account the increasing importance of digestive enzyme inhibitors as insecticidal agents, the entomopathogenic potential of the fractions obtained was evaluated against spodoptera litura (fab.), a polyphagous pest. considerable mortality was obtained when the larvae were fed on diet supplemented with the partially purif ...201627265826
siderophore biosynthesis but not reductive iron assimilation is essential for the dimorphic fungus nomuraea rileyi conidiation, dimorphism transition, resistance to oxidative stress, pigmented microsclerotium formation, and virulence.iron is an indispensable factor for the dimorphic insect pathogenic nomuraea rileyi to form persistent microsclerotia which can replace conidia or blastospores for commercial mass production. there are two high affinity iron acquisition pathways in n. rileyi, siderophore-assisted iron mobilization and reductive iron assimilation systems. transcription of the two iron uptake pathways related genes is induced under iron-limiting conditions. stage-specific iron uptake-related genes expression durin ...201627379061
pheromone lure and trap color affects bycatch in agricultural landscapes of utah.aerial traps, using combinations of color and attractive lures, are a critical tool for detecting and managing insect pest populations. yet, despite improvements in trap efficacy, collection of nontarget species ("bycatch") plagues many insect pest surveys. bycatch can influence survey effectiveness by reducing the available space for target species and increasing trap screening time, especially in areas where thousands of insects are captured as bycatch in a given season. additionally, bycatch ...201627412193
insecticidal activity and histopathological effects of vip3aa protein from bacillus thuringiensis on spodoptera litura.vegetative insecticidal proteins (vips) are insecticidal proteins synthesized by bacillus thuringiensis during the vegetative stage of growth. in this study, vip3aa protein, obtained by in vitro expression of the vip3aa gene from b. thuringiensis wb5, displayed high insecticidal activity against spodoptera litura aside from spodoptera exigua and helicoverpa armigera. bioassay results showed that the toxicity of vip3aa protein against s. litura larvae statistically decreased along with the increa ...201627435544
the generalist inside the specialist: gut bacterial communities of two insect species feeding on toxic plants are dominated by enterococcus sp.some specialist insects feed on plants rich in secondary compounds, which pose a major selective pressure on both the phytophagous and the gut microbiota. however, microbial communities of toxic plant feeders are still poorly characterized. here, we show the bacterial communities of the gut of two specialized lepidoptera, hyles euphorbiae and brithys crini, which exclusively feed on latex-rich euphorbia sp. and alkaloid-rich pancratium maritimum, respectively. a metagenomic analysis based on hig ...201627446044
target and non-target toxicity of botanical insecticide derived from couroupita guianensis l. flower against generalist herbivore, spodoptera litura fab. and an earthworm, eisenia foetida savigny.botanical insecticides may provide alternatives to synthetic insecticides for controlling spodoptera litura (f.) and they are target specific, biodegradable, and harmless to mammals. eight natural chemical compounds with larvicidal activity were identified from fraction f6 of c. guianensis flower extract. probit analysis of 95% confidence level exposed an lc50 of 223ppm against s. litura third instar larvae. the growth and development of s. litura was affected in sub-lethal concentrations of fra ...201627476000
toxicity and physiological effect of quercetin on generalist herbivore, spodoptera litura fab. and a non-target earthworm eisenia fetida savigny.a novel flavonoid, quercetin, was isolated from the medicinal plant euphorbia hirta l. through chromatography techniques including: tlc, column chromatography, nmr and then screened for toxicity to larvae of spodoptera litura fab. bioassays were used to analyze pupal weight, survival rate, fecundity, egg hatchability, population growth index, nutritional index and histopathology of treated larvae at a range of e. hirta extract concentrations. results of toxicity assays demonstrated that, 6 ppm o ...201627657818
molecular cloning and characterization of α-amylase/subtilisin inhibitor from rhizome of ligusticum chuanxiong.to clone and characterize a novel bi-functional α-amylase/subtilisin inhibitor (lasi) from the rhizome of ligusticum chuanxiong, a traditional chinese medicine.201727752792
inducible de novo biosynthesis of isoflavonoids in soybean leaves by spodoptera litura derived elicitors: tracer techniques aided by high resolution lcms.isoflavonoids are a characteristic family of natural products in legumes known to mediate a range of plant-biotic interactions. for example, in soybean (glycine max: fabaceae) multiple isoflavones are induced and accumulate in leaves following attack by spodoptera litura (lepidoptera: noctuidae) larvae. to quantitatively examine patterns of activated de novo biosynthesis, soybean (var. enrei) leaves were treated with a combination of plant defense elicitors present in s. litura gut content extra ...201627826811
spodoptera litura autophagy-related protein 1 interacts with autophagy-related protein 5 and enhances its degradation.it is known that the autophagy-related protein 1 (atg1) plays critical roles in the regulation of autophagy in mammals and yeast, whereas the function of atg1 in lepidopteran insects is not well elucidated. here spodoptera litura atg1 (slatg1) and its interactions with other atg proteins were characterized. alternative splicing of slatg1 produced at least four transcript variants. over-expression and rna interference knockdown of slatg1 demonstrated that slatg1 enhanced autophagy. slatg1a-green ...201727902874
antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of markhamia tomentosa.plants harbor endophytes with potential bioactivity. markhamia tomentosa (benth) k. schum ex. engl. (bignoniaceae) is reported to possess antioxidant, anti-inflammatory and anticancer activities.201727937112
molecular dynamics and protein interaction studies of lipopeptide (iturin a) on α- amylase of spodoptera litura.the small mottled willow moth (spodoptera litura) is one of the best-known agricultural pest insects. to understand the insecticidal activity, we have selected iturin a compound produced by bacillus amyloliquefaciens rhnk22 which showed the strongest and most common inhibitory effect on the spodoptera litura protein. in this work we have identified the action of iturin a on α- amylase is a major digestive enzyme of spodoptera litura using docking studies. a 3d model of α- amylase from spodoptera ...201727940096
characterization of putative virulence factors of serratia marcescens strain sen for pathogenesis in spodoptera litura.two serratia marcescens strains, sen and icc-4, isolated from diseased insect cadavers were observed to differ considerably in their virulence towards spodoptera litura. the present study was aimed to characterize the possible virulence factors present in the virulent serratia marcescens strain sen. both the s. marcescens strains were evaluated for the presence of various lytic enzymes such as chitinase, lipase, protease and phospholipase. the virulent s. marcescens strain sen was observed to po ...201727993620
plant defence induced by pgpr against spodoptera litura in tomato (solanum lycopersicum l.).the research conducted including its rationale: spodoptera litura is the major pest of tomato causing significant reduction in tomato yield. application of plant growth promoting rhizobacteria(pgpr) prevent use of chemical fertilizer and synthetic pesticides through enhancement of plant growth and yield and induction of systemic resistance. present investigation is an attempt to evaluate the role of pgpr, pseudomonas putida and rothia sp. on the physiology and yield of tomato fruit infested with ...201728004873
weeding volatiles reduce leaf and seed damage to field-grown soybeans and increase seed isoflavones.field experiments were conducted over 3 years (2012, 2013, and 2015), in which half of the young stage soybean plants were exposed to volatiles from cut goldenrods three times over 2-3 weeks, while the other half remained unexposed. there was a significant reduction in the level of the total leaf damage on exposed soybean plants compared with unexposed ones. in 2015, the proportion of damage to plants by spodoptera litura larvae, a dominant herbivore, was significantly less in the exposed field ...201728134284
responses of the cutworm spodoptera litura (lepidoptera: noctuidae) to two bt corn hybrids expressing cry1ab.to examine the responses of the secondary lepidopteran pest spodoptera litura to two bacillus thuringiensis (bt) corn hybrids [5422bt1 (event bt11), 5422cbcl (mon810)] expressing cry1ab, larval bioassays with cry1ab toxin, corn leaves or kernels and bagging on corn plants were conducted. the results showed that larvae displayed a similar performance when fed kernels, but not leaves of 5422bt1, 5422cbcl and their near-isogenic non-bt corn (5422). significantly higher cry1ab amounts were detected ...201728186125
characterization of parasporin gene harboring indian isolates of bacillus thuringiensis.bacillus thuringiensis (bt) is popularly known as insecticidal bacterium. however, non-insecticidal bt strains are more extensively available in natural environment than the insecticidal ones. parasporin (ps) is a collection of genealogically heterogeneous cry proteins synthesized in non-insecticidal isolates of bt. an important character generally related with ps proteins is their strong cytocidal activity preferentially on human cancer cells of various origins. identification and characterizat ...201428324389
differential expression of chemosensory-protein genes in midguts in response to diet of spodoptera litura.while it has been well characterized that chemosensory receptors in guts of mammals have great influence on food preference, much remains elusive in insects. insect chemosensory proteins (csps) are soluble proteins that could deliver chemicals to olfactory and gustatory receptors. recent studies have identified a number of csps expressed in midgut in lepidoptera insects, which started to reveal their roles in chemical recognition and stimulating appetite in midgut. in this study, we examined exp ...201728331183
economic injury level and demography-based control timing projection of spodoptera litura (lepidoptera: noctuidae) at different growth stages of arachis hypogaea.spodoptera litura (f.), one of the most devastating pests in many asian countries, is normally controlled by relying on chemical insecticides. to encourage an integrated pest management approach, we determined the economic injury level (eil) for s. litura on peanut, arachis hypogaea l., by larval infestation with late instars at different crop growth stages. the cumulative consumption rate of the fifth- and sixth-instars was used as the relative unit for the "spodoptera injury equivalent" (sie). ...201728334106
pre-exposures to taro (colocasia esculenta) leaf volatiles enhance the reproductive behaviors in spodoptera litura.plant volatiles serve as sensory cues for insects to find food and habitats. they are also reported to affect many insect behaviors. in the current study, we determined how exposures to taro (colocasia esculenta) for 24h affect reproductive behaviors in spodoptera litura. further, we investigated the mechanisms that regulate taro volatiles-induced reproductive behavior in s. litura by recording peripheral, behavioral, and neuronal responses of male moths to sex pheromone components (z9,e11-14:oa ...201728336369
20-hydroxyecdysone positively regulates the transcription of the antimicrobial peptide, lebocin, via bmets and bmbr-c z4 in the midgut of bombyx mori during metamorphosis.metamorphosis is an essential physiological process in insects. this process is triggered by 20-hydroxyecydsone (20e). lebocin, an antimicrobial peptide of lepidoptera insects, was significantly up-regulated in the midgut, but not in the fat body of bombyx mori during metamorphosis. in this study, the expression regulation of lebocin in b. mori midgut was studied. the results showed that b. mori lebocin and its activator bmets were not responsive to bacterial infection in the midgut, instead, th ...201728390932
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