Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| rhinocerebral zygomycosis in a patient with acute lymphoblastic leukemia. | we report a case of a 28-year-old man with acute lymphoblastic leukemia who developed rhinocerebral zygomycosis during induction chemotherapy. this life-threatening fungal infection is an infrequent cause of neutropenic fever, and is occasionally found in patients with leukemia and lymphoma, or patients with severely compromised defence mechanisms due to other diseases. it is caused by moulds belonging to the mucoraceae family, and is characterized by local destruction of the affected organ. in ... | 2001 | 11332157 |
| diguanidino and "reversed" diamidino 2,5-diarylfurans as antimicrobial agents. | dicationic 2,5-bis(4-guanidinophenyl)furans 5a-5f, 2,5-bis[4-(arylimino)aminophenyl]furans 6a-6b and 6e-6k, and 2,5-bis[4-(alkylimino)aminophenyl]furans 6c-6d have been synthesized starting from 2,5-bis[tri-n-butylstannyl]furan. thermal melting studies with poly da*dt and the duplex oligomer d(cgcgaattcgcg)2 demonstrated high dna binding affinities for a number of the compounds. the binding affinities are highly dependent on structure and are significantly affected by substituents both on the ph ... | 2001 | 11356109 |
| whole cell biocatalyst for biodiesel fuel production utilizing rhizopus oryzae cells immobilized within biomass support particles. | as part of a research program aimed at producing biodiesel fuel from plant oils enzymatically cells of rhizopus oryzae (r. oryzae) ifo4697 (with a 1,3-positional specificity lipase) immobilized within biomass support particles (bsps) were investigated for the methanolysis of soybean oil. the r. oryzae cells easily became immobilized within the bsps during batch operation. to enhance the methanolysis activity of the immobilized cells under the culture conditions used, various substrate-related co ... | 2001 | 11356369 |
| cloning and expression analysis of nhl1, a gene encoding an extracellular lipase from the fungal pea pathogen nectria haematococca mp vi (fusarium solani f. sp. pisi) that is expressed in planta. | the filamentous fungus nectria haematococca (anamorph fusarium solani f. sp. pisi) resides in soil, and attacks pea seedlings in the area of the underground epicotyl and upper tap root, causing foot rot disease. we detected lipase activity during in vitro growth of n. haematococca. subsequently, a lipase gene was cloned and functionally characterised by heterologous expression in saccharomyces cerevisiae. the full-length cdna of 1152 bp was cloned using a 3' race-pcr approach coupled with cdna l ... | 2001 | 11361331 |
| function of the prosequence for in vivo folding and secretion of active rhizopus oryzae lipase in saccharomyces cerevisiae. | the role of the prosequence of rhizopus oryzae lipase (rol) with a preprosequence was analyzed by an expression system using saccharomyces cerevisiae. when the mature portion of rol (mrol) fused to the pre-alpha-factor leader sequence was expressed, secretion of active mrol was not observed. however, when mrol was synthesized together with the prosequence in trans (individually and coincidentally), secretion of active mrol was observed. the results indicate that the prosequence of rol helped cor ... | 2001 | 11398926 |
| study of lipase-catalyzed hydrolysis of some monoterpene esters. | studies of the hydrolysis of bornyl, citronellyl, geranyl, and terpenyl acetates by commercially available lipases of candida rugosa, rhizopus arrhizus, and mucor miehei are presented. the hydrolysis of these monoterpene esters is investigated at various temperatures and phs, and the time dependence of the percentage of esters hydrolysed is studied. the catalytic activities of the lipases in hydrolysing the esters are compared and, overall, it is observed that under the experimental conditions u ... | 2001 | 11400729 |
| stability and function of interdomain linker variants of glucoamylase 1 from aspergillus niger. | several variants of glucoamylase 1 (ga1) from aspergillus niger were created in which the highly o-glycosylated peptide (aa 468--508) connecting the (alpha/alpha)(6)-barrel catalytic domain and the starch binding domain was substituted at the gene level by equivalent segments of glucoamylases from hormoconis resinae, humicola grisea, and rhizopus oryzae encoding 5, 19, and 36 amino acid residues. variants were constructed in which the h. resinae linker was elongated by proline-rich sequences as ... | 2001 | 11478902 |
| kinetic studies of rhizopus oryzae lipase using monomolecular film technique. | rhizopus oryzae lipase (rol) was found to be a true lipase. this enzyme presents the interfacial activation phenomenon. the n-terminal amino acid sequence of rol was compared to those of rhizopus lipases. purified rol possesses the same n-terminal sequence as the mature rhizopus niveus lipase (rnl). this sequence was found in the last 28 amino acids of the propeptide sequence derived from the cdna of rhizopus delemar lipase (rdl). using the baro-stat method, we have measured the hydrolysis rate ... | 2001 | 11506890 |
| blends of aliphatic polyesters. vi. lipase-catalyzed hydrolysis and visualized phase structure of biodegradable blends from poly(epsilon-caprolactone) and poly(l-lactide). | phase-separated biodegradable polymer blends were prepared from poly(epsilon-caprolactone) (pcl) and poly(l-lactide) (plla), and rhizopus arrhizus lipase-catalyzed hydrolysis and phase structure of the blend films were investigated. gravimetry revealed that the lipase-catalyzed hydrolysis of pcl in pcl- and plla-rich phases is disturbed by the presence of plla. polarimetry confirmed the occurrence of a predominant hydrolysis of pcl and subsequent removal of the hydrolyzed water-soluble pcl oligo ... | 2001 | 11518579 |
| ten years' experience in zygomycosis at a tertiary care centre in india. | to define the spectrum of zygomycosis due to mucorales in an indian scenario. | 2001 | 11545569 |
| antifungal activities and cytotoxicity studies of six new azasordarins. | gw 471552, gw 471558, gw 479821, gw 515716, gw 570009, and gw 587270 are members of a new family of sordarin derivatives called azasordarins. the in vitro activities of these compounds were evaluated against clinical isolates of yeasts, including candida albicans, candida non-albicans, and cryptococcus neoformans strains. activities against pneumocystis carinii, aspergillus spp., less common molds, and dermatophytes were also investigated. azasordarin derivatives displayed significant activities ... | 2001 | 11600368 |
| spacer-mediated display of active lipase on the yeast cell surface. | we have constructed a saccharomyces cerevisiae strain displaying an active lipase on the cell surface by cell surface engineering. the gene encoding rhizopus oryzae lipase (rol) was fused with the genes encoding the pre-alpha-factor leader sequence and the c-terminal half of alpha-agglutinin including the glycosylphosphatidylinositol-anchor attachment signal. the constructed gene was overexpressed under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter. linker peptides (spacer ... | 2001 | 11601614 |
| efficient and selective microbial esterification with dry mycelium of rhizopus oryzae. | the use of dry mycelium of rhizopus oryzae as biocatalyst for ester production in organic solvent has been studied. mycelia with notable carboxylesterase activity were produced when different tweens (20, 40, 60 and 80) were employed as main carbon source for the growth. dry mycelium of four strains of rhizopus oryzae proved effective for efficiently catalysing the synthesis of different flavour esters (hexylacetate and butyrate, geranylacetate and butyrate) starting from the corresponding alcoho ... | 2001 | 11604169 |
| chemoenzymatic synthesis of antiviral carbocyclic nucleosides: asymmetric hydrolysis of meso-3,5-bis(acetoxymethyl)cyclopentenes using rhizopusdelemar lipase. | 7-substituted norbornadienes were stereoselectively converted into the meso-3,5-bis(acetoxymethyl)cyclopentenes by a three-step sequence of ozonolysis, reduction, and acetylation. rhizopus delemar lipase (rdl)-catalyzed asymmetric hydrolysis of meso-3,5-bis(acetoxymethyl)cyclopentenes afforded the monoalcohols of high enantiomeric purities (>95% ee) in good yields (64-95%). the obtained monoalcohols 11 and 14 could be applied for the synthesis of antiviral carbocyclic nucleosides (-)-carbovir an ... | 1996 | 11667592 |
| microbiological oxygenation of bridgehead azabicycloalkanes. | a series of n-substituted bridgehead azabicycloalkanes has been prepared and examined as substrates for microbiological oxygenation using the fungi beauveria bassiana, rhizopus nigricans, aspergillus ochraceus, and rhizopus arrhizus. oxygenation using b. bassiana of n-tosyl-7-azabicyclo[2.2.1]heptane gave n-[p-(hydroxymethyl)benzenesulfonyl]-7-azabicyclo[2.2.1]heptane (56% yield), of n-(phenyloxycarbonyl)-7-azabicyclo[2.2.1]heptane gave the 2-endo-ol (56% yield, 51% ee), of n-boc-7-azabicyclo[2. ... | 1997 | 11671535 |
| structurally novel bioconversion products of the marine natural product sarcophine effectively inhibit jb6 cell transformation. | sarcophytol a (1) and b (2) (see chart 1) are cembrane-type diterpenes known to inhibit tumor promotion. indicative of this inhibitory response, we currently report sarcophytol a (1) mediates dose-dependent diminution of 12-o-tetradecanoylphorbol 13-acetate (tpa)-induced transformation of jb6 cells. moreover, a structurally related furanocembrane diterpene, sarcophine (3), isolated in good yield from the red sea soft coral sarcophyton glaucum, was also found to serve as an effective inhibitor of ... | 1998 | 11672397 |
| animal models of zygomycosis--absidia, rhizopus, rhizomucor, and cunninghamella. | infections caused by zygomycetes, which have been increasing in recent years, are known for their difficulty of diagnosis and treatment. because little is known about this fungus and its infection, vigorous research is now in serious demand. as in many other systemic mycoses, animal model studies are essential in the investigation of zygomycosis, particularly for the study of pathogenesis, diagnosis and treatment. unfortunately, such studies have been limited when compared with those of aspergil ... | 2001 | 11694096 |
| synergy, pharmacodynamics, and time-sequenced ultrastructural changes of the interaction between nikkomycin z and the echinocandin fk463 against aspergillus fumigatus. | we investigated the potential synergy between two cell wall-active agents, the echinocandin fk463 (fk) and the chitin synthase inhibitor nikkomycin z (nz), against 16 isolates of filamentous fungi. susceptibility testing was performed with a broth macrodilution procedure by nccls methods. the median minimal effective concentration (mec) of fk against all aspergillus species was 0.25 microg/ml (range, 0.05 to 0.5 microg/ml). for fusarium solani and rhizopus oryzae, mecs of fk were >512 microg/ml. ... | 2001 | 11709302 |
| hyperbaric oxygen as an adjunct in zygomycosis: randomized controlled trial in a murine model. | zygomycosis was induced by injecting cd-1 mice with 5 mg of intraperitoneal deferoxamine and then 10(6) cfu of intravenous and intrasinus rhizopus arrhizus. the addition of hyperbaric oxygen (2.0 atm absolute twice daily) to amphotericin b did not improve survival over that achieved with amphotericin b and placebo air treatments. | 2001 | 11709348 |
| nutritional influence on fungal colony growth and biomass distribution in response to toxic metals. | this work examines nutritional influence on fungal colony growth and biomass distribution in response to toxic metals. in low-substrate solid medium, 0.1 mm cd, cu and zn caused a decrease in radial expansion of both trichoderma viride and rhizopus arrhizus. however, as the amount of available carbon source (glucose) increased, the apparent toxicity of the metals decreased. these metals also affected the overall length of the fungal mycelium and branching patterns. in low-nutrient conditions, t. ... | 2001 | 11731141 |
| lipase-catalysed hydrolysis of short-chain substrates in solution and in emulsion: a kinetic study. | we have studied the enzymatic hydrolysis of solutions and emulsions of vinyl propionate, vinyl butyrate and tripropionin by lipases of various origin and specificity. kinetic studies of the hydrolysis of short-chain substrates by microbial triacylglycerol lipases from rhizopus oryzae, mucor miehei, candida rugosa, candida antarctica a and by (phospho)lipase from guinea-pig pancreas show that these lipolytic enzymes follow the michaelis-menten model. surprisingly, the activity against solutions o ... | 2001 | 11750885 |
| yeast whole-cell biocatalyst constructed by intracellular overproduction of rhizopus oryzae lipase is applicable to biodiesel fuel production. | yeast whole-cell biocatalysts for lipase-catalyzed reactions were constructed by intracellularly overproducing rhizopus oryzae lipase (rol) in saccharomvces cerevisiae mt8-1. the gene encoding lipase from r. orvzae ifo4697 was cloned, and intracellular overproduction systems of a recombinant rol with a pro-sequence (rprorol) were constructed. when rprorol from r. oryzae ifo4697 was produced under the control of the 5'-upstream region of the isocitrate lyase gene of candida tropicalis (upr-icl) a ... | 2001 | 11762598 |
| adsorption properties and activities of lipase on a gold substrate modified by self-assembled monolayers. | the adsorption properties, amount and specific activity of lipase d from rhizopus delemar were investigated by employing a gold substrate modified with seven kinds of thiol monolayer. quartz crystal microbalance measurements revealed that the amount of the enzyme adsorbed to the hydrophobic monolayers (e.g. benzenethiol) was much higher than that to the hydrophilic monolayers (e.g. 3-mercaptopropanoic acid). in contrast, lipase d adsorbed to the hydrophilic, 2-amino-1-ethanethiol monolayer showe ... | 2001 | 11791710 |
| biosorption of heavy metals using whole mold mycelia and parts thereof. | biosorption of heavy metals was carried out using whole mycelia and selected components of aspergillus niger, rhizopus oryzae and mucor rouxii. binding of copper, cadmium, nickel and zinc was considerably improved by treating the cell wall fraction with 4 m naoh at 121 degrees c. chitosan contributed most to the biosorptive capacity. 0.96 mmol copper was bound by 1 g of the treated mycelium of m. rouxii dsm 1191. | 2002 | 11800481 |
| case report. fatal rhinocerebral zygomycosis due to rhizopus oryzae. | a case of rhinocerebral zygomycosis due to rhizopus oryzae, arising after trauma in a 53-year-old diabetic man, is reported. diagnosis was based on histological and mycological examination. fragments of the colonies were observed by scanning electron microscopy. this is the first case diagnosed in tuscany. | 2002 | 11856437 |
| formation of and coalescence from the inclusion complex of a biodegradable block copolymer and alpha-cyclodextrin. 2: a novel way to regulate the biodegradation behavior of biodegradable block copolymers. | a biodegradable block copolymer (pcl-b-plla, m(n) = 1.72 x 10(4), m(w)/m(n) = 1.37) of poly(epsilon-caprolactone) (pcl) and poly(l-lactide) (plla) with very low crystallinity was obtained by forming the inclusion complex between alpha-cyclodextrin molecules and pcl-b-plla followed by coalescence of the guest polymer chains. films of the as-synthesized and coalesced copolymer samples, pcl and plla homopolymers of approximately the same chain lengths as the corresponding blocks of pcl-b-plla, and ... | 2002 | 11866574 |
| optimization of some additives to improve protease production under ssf. | in a locally isolated rhizopus oryzae strain highest-production of protease (388.54/g wheat bran) was observed in presence of tween-80 and dioctyl sodium sulfosuccinate individually at 40mg/g wheat bran concentration. under solid state fermentation biotin (0.0025mg/g wheat bran); ca2+ (0.05mg/g wheat bran) and 1-naphthyl acetic acid (0.01mg/g wheat bran) also showed some inducing effect on the synthesis of the enzyme protease by solid state fermentation. | 2001 | 11906108 |
| synthesis of phosphatidylcholine with defined fatty acid in the sn-1 position by lipase-catalyzed esterification and transesterification reaction. | the incorporation of caproic acid in the sn-1 position of phosphatidylcholine (pc) catalyzed by lipase from rhizopus oryzae was investigated in a water activity-controlled organic medium. the reaction was carried out either as esterification or transesterification. a comparison between these two reaction modes was made with regard to product yield, product purity, reaction time, and byproduct formation as a consequence of acyl migration. the yield in the esterification and transesterification re ... | 2002 | 11948447 |
| fluorescent inhibitors reveal solvent-dependent micropolarity in the lipid binding sites of lipases. | triacylglycerol analogue p-nitrophenyl phosphonates specifically react with the active-site serine of lipolytic enzymes to give covalent lipase-inhibitor complexes, mimicking the first transition state which is involved in lipase-mediated ester hydrolysis. here we report on a new type of phosphonate inhibitors containing a polarity-sensitive fluorophore to monitor micropolarity around the active site of the enzyme in different solvents. the respective compounds are hexyl and methyl dimethylamino ... | 2002 | 12009403 |
| in vitro activity of fk463, a novel lipopeptide antifungal agent, against a variety of clinically important molds. | the in vitro antifungal activity of fk463 against a variety of clinically important opportunistic molds was compared with amphotericin b, itraconazole and fluconazole by using the broth microdilution method m27-a specified by the national committee for clinical laboratory standards. fk463 exhibited potent activity against aspergillus species, which was superior to those of all other compounds tested. fk463 was also active against the dematiaceous fungi cladosporium trichoides, exophiala spinifer ... | 2002 | 12011539 |
| gas-phase enzymatic esterification on immobilized lipases in mcm-41 molecular sieves. | several lipolytic enzymes were immobilized in the pores of mcm-41 and al-mcm-41 molecular sieves and used as catalysts in the gas-phase esterification of acetic acid with ethanol. the entrapment of enzymes depended on the molecular sieve and the type of enzyme used. the order of enzymatic activity for enzymes entrapped in the pores of mcm-41 and al-mcm-41 in the esterification reaction was of (rhizopus niveus lipases) < fap-15 (rhizopus oryzae lipases) < lex (mucorjavanicus lipases) < ps (pseudo ... | 2002 | 12018317 |
| lactic acid fermentation of potato pulp by the fungus rhizopus oryzae. | thirty-eight strains of the fungus rhizopus oryzae were grown on potato pulp, an agricultural by-product of the starch industry. either lactic acid or fumaric acid and ethanol were formed, and the ratio differed among the strains tested. the highest amount of l(+)-lactic acid (10 mg/g fresh matter) was observed in the pulp fermented for six days by rhizopus oryzae ifo 4707. the ifo 4707 strain rapidly reduced the hardness and ph of potato pulp within one day followed by the gradual synthesis of ... | 2002 | 12029519 |
| comparison of the effects of temperature and water activity on growth rate of food spoilage moulds. | the influence of temperature (t) and water activity (aw) on the growth rate (mu) of seven moulds (alternaria alternata, aspergillus flavus, cladosporium cladosporioides, mucor racemosus, penicillium chrysogenum, rhizopus oryzae and trichoderma harzianum) was assessed in suboptimal conditions. firstly, the dependence of fungal growth on temperature, at aw 0.99, was modelled through an approach described previously for bacteria. a dimensionless growth rate variable: mu(dimalpha)=mu/mu (optalpha) d ... | 2002 | 12032803 |
| enhanced separation of filamentous fungi by ultrasonic field: possible usage in repeated batch processes. | usage of ultrasonic field-based filters in retention of filamentous fungal cells was assessed using rhizopus arrhizus nrrl 1526 as a model organism. effects of operating conditions, such as power input, harvest pump flow rate, run time and stop time, on the system's separation efficiency (se) were evaluated by modulating the variables according to a central composite design (ccd). the standard pump with which the ultrasonic filter was equipped was shown to be unsuitable and was, therefore, subst ... | 2002 | 12067525 |
| fungal chitosan production and its characterization. | the objective of this investigation was to evaluate the chitosans produced by several species of fungi. | 2002 | 12081543 |
| copper complexes with bioactive ligands. part ii--antifungal activity. | antifungal activity of new copper(ii) complexes of 2-methylthionicotinate (2-mesnic) of the composition cu(2-mesnic)2(menia)(2).4h2o (where menia is n-methylnicotinamide), and cu(2-mesnic)2(nia)(2).2h2o (where nia is nicotinamide) and cu(2-mesnic)2l2 (where l is isonicotinamide, inia, or ethyl nicotinate, etnic) were tested on various strains of filamentous fungi by the macrodilution method. most sensitive against copper(ii) adducts with bioactive ligands were rhizopus oryzae and microsporum gyp ... | 2002 | 12094729 |
| direct surface analysis of fungal species by matrix-assisted laser desorption/ionization mass spectrometry. | in this study various methods of sample preparation and matrices were investigated to determine optimum collection and analysis criteria for fungal analysis by matrix-assisted laser desorption/ionization mass spectrometry (maldi-ms). intact spores and/or hyphae of aspergillus niger, rhizopus oryzae, trichoderma reesei and phanerochaete chrysosporium were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms). the fungal samples were applied to the ... | 2002 | 12112615 |
| fungal infections in marrow transplant recipients under antifungal prophylaxis with fluconazole. | fungal infection is one of the most important causes of morbidity and mortality in bone marrow transplant (bmt) recipients. the growing incidence of these infections is related to several factors including prolonged granulocytopenia, use of broad-spectrum antibiotics, conditioning regimens, and use of immunosuppression to avoid graft-versus-host disease (gvhd). in the present series, we report five cases of invasive mold infections documented among 64 bmt recipients undergoing fluconazole antifu ... | 2002 | 12131918 |
| construction of yeast strains with high cell surface lipase activity by using novel display systems based on the flo1p flocculation functional domain. | we constructed a novel cell-surface display system, using as a new type of cell-wall anchor 3,297 or 4,341 bp of the 3' region of the flo1 gene (fs or fl gene, respectively), which encodes the flocculation functional domain of flo1p. in this system, the n terminus of the target protein was fused to the fs or fl protein and the fusion proteins were expressed under the control of the inducible promoter upr-icl (5' upstream region of the isocitrate lyase of candida tropicalis). using this new syste ... | 2002 | 12200308 |
| production of l(+)-lactic acid from glucose and starch by immobilized cells of rhizopus oryzae in a rotating fibrous bed bioreactor. | a rotating fibrous-bed bioreactor (rfb) was developed for fermentation to produce l(+)-lactic acid from glucose and cornstarch by rhizopus oryzae. fungal mycelia were immobilized on cotton cloth in the rfb for a prolonged period to study the fermentation kinetics and process stability. the ph and dissolved oxygen concentration (do) were found to have significant effects on lactic acid productivity and yield, with ph 6 and 90% do being the optimal conditions. a high lactic acid yield of 90% (w/w) ... | 2002 | 12209781 |
| the role of ligand exchange in the uptake of iron from microbial siderophores by gramineous plants. | the siderophore rhizoferrin, produced by the fungus rhizopus arrhizus, was previously found to be as an efficient fe source as fe-ethylenediamine-di(o-hydroxphenylacetic acid) to strategy i plants. the role of this microbial siderophore in fe uptake by strategy ii plants is the focus of this research. fe-rhizoferrin was found to be an efficient fe source for barley (hordeum vulgare l.) and corn (zea mays l.). the mechanisms by which these gramineae utilize fe from fe-rhizoferrin and from other c ... | 1996 | 12226445 |
| removal of heavy metals from aqueous solution using rhizopus delemar mycelia in free and polyurethane-bound form. | this study assesses the ability of mycelia of rhizopus delemar (both free and immobilized on polyurethane foam) to remove heavy metals from single-ion solutions as well as from a mixture of them. all experiments were conducted using 0.5-5 mm solutions of cuso4 x 5h2o, cocl2-6h2o and feso4 7h2o. mycelia immobilized on polyurethane foam cells showed some times increase in uptake compared with that of free cells. metal ions accumulation from a mixed solution was decreased slightly for cobalt and ir ... | 2002 | 12240988 |
| homologous recombination and double-strand break repair in the transformation of rhizopus oryzae. | genetic transformation of the mucorales fungi has been problematic, since dna transformed into the host rarely integrates and usually is mitotically unstable in the absence of selective pressure. in this study, transformation of rhizopus oryzae was investigated to determine if the fate of introduced dna could be predicted based on double-strand break repair and recombination mechanisms found in other fungi. a transformation system was developed with uracil auxotrophs of rhizopus oryzae that coul ... | 2002 | 12436261 |
| microbial and chemical transformation studies of the bioactive marine sesquiterpenes (s)-(+)-curcuphenol and -curcudiol isolated from a deep reef collection of the jamaican sponge didiscus oxeata. | microbial and chemical transformation studies of the marine sesquiterpene phenols (s)-(+)-curcuphenol (1) and (s)-(+)-curcudiol (2), isolated from the jamaican sponge didiscus oxeata, were accomplished. preparative-scale fermentation of 1 with kluyveromyces marxianus var. lactis (atcc 2628) has resulted in the isolation of six new metabolites: (s)-(+)-15-hydroxycurcuphenol (3), (s)-(+)-12-hydroxycurcuphenol (4), (s)-(+)-12,15-dihydroxycurcuphenol (5), (s)-(+)-15-hydroxycurcuphenol-12-al (6), (s) ... | 2002 | 12444675 |
| long anchor using flo1 protein enhances reactivity of cell surface-displayed glucoamylase to polymer substrates. | we investigated the influence of anchor length on the reactivity to polymer substrate of enzyme displayed on yeast cell surfaces. using various lengths [42, 102, 146, 318, 428, and 1,326 amino acids (aa)] of the c-terminal region of the saccharomyces cerevisiae flo1 protein (flo1p), which plays a major role in yeast flocculation, six display systems with various anchor lengths were constructed. in these systems, the target protein was displayed on the yeast cell surface under the control of the ... | 2002 | 12466889 |
| in vitro susceptibilities of zygomycetes to conventional and new antifungals. | in vitro susceptibilities of 36 zygomycete isolates, belonging to six genera, to itraconazole, posaconazole, voriconazole, terbinafine, amphotericin b and 5-fluorocytosine were determined by using a broth microdilution adaptation of the national committee for clinical laboratory standards m-38p reference method. the influence of incubation time on mic values, and the performance of a spectrophotometric method for mic determination in comparison with the visual reference method, were also evaluat ... | 2003 | 12493786 |
| [determination of lactic acid in fermentation broth of rhizopus oryzae by reversed-phase high performance liquid chromatography (rp-hplc)]. | a method for determining lactic acid in fermentation broth of rhizopus oryzae by rp-hplc is described. the operating conditions were wakosil-ii 5 c18 rs column(4.6 mm i.d. x 150 mm, 5 microns) at room temperature, 0.01 mol/l phosphoric acid solution (ph 2.5) as mobile phase with a flow rate of 1.0 ml/min and uv detection at 210 nm. the retention time of lactic acid was 3.820 min. this method is simple, rapid and accurate. the results will not be affected by other components in the broth. the rel ... | 2000 | 12541741 |
| [determination of enantiomeric purity for lactic acid in fermentation broth by rhizopus oryzae with high performance liquid chromatography]. | a procedure for the resolution of dl-lactic acid and the determination of d-isomer ratio in l-lactic acid fermentation broth by rhizopus oryzae is described. the effects of ph of mobile phase and concentration of chiral mobile phase additives on resolution of dl-lactic acid were investigated. the optical isomers of lactic acid were resolved by rp-hplc with 2,3,6-tri-o-beta-cyclodextrin(tm-beta-cd) as a chiral mobile phase additive, and c18 column as stationary phase, and detected at wavelength 2 ... | 2001 | 12541838 |
| lactic acid production by saccharomyces cerevisiae expressing a rhizopus oryzae lactate dehydrogenase gene. | this work demonstrates the first example of a fungal lactate dehydrogenase (ldh) expressed in yeast. a l(+)-ldh gene, ldha, from the filamentous fungus rhizopus oryzae was modified to be expressed under control of the saccharomyces cerevisiae adh1 promoter and terminator and then placed in a 2 micro -containing yeast-replicating plasmid. the resulting construct, pldha68x, was transformed and tested by fermentation analyses in haploid and diploid yeast containing similar genetic backgrounds. both ... | 2003 | 12545382 |
| [l-lactic acid fermentation by immobilized rhizopus oryzae in a three-phase fluidized-bed]. | l-lactic acid fermentation in a three-phase fluidized-bed bioreactor was studied by using immobilized rhizopus oryzae cells with polyurethane particles as supporter. the results indicated that the immobilized cells were stable after used for 8 batches repeatedly. the rate and the yield of lactic acid fermentation with glucose as carbon source were 19.1 g h-1.l (bead)-1 and 82.5%, respectively. with the hydrolyzate of corn cob residue from xylose manufacturer by cellulase hydrolysis, the yield of ... | 2000 | 12548964 |
| molecular cloning of endo-beta-d-1,4-glucanase genes, rce1, rce2, and rce3, from rhizopus oryzae. | three endoglucanase genes, designated the rce1, rce2, and rce3 genes, were isolated from rhizopus oryzae as the first cellulase genes from the subdivision zygomycota: all the amino acid sequences deduced from the rce1, rce2, and rce3 genes consisted of three distinct domains: cellulose binding domains, linker domains, and catalytic domains belonging to glycosyl hydrolase family 45. the rce3 gene had two tandem repeated sequences of cellulose binding domains, while rce1 and rce2 had only one. rce ... | 2003 | 12591897 |
| production of mycelium biomass and ethanol from paper pulp sulfite liquor by rhizopus oryzae. | the cultivation conditions for rhizopus oryzae grown in synthetic medium and paper pulp spent sulfite liquor (ssl) were investigated to achieve high biomass and ethanol yields using shake flasks and bioreactors. the fungus assimilated the hexoses glucose, mannose and galactose, and the pentoses xylose and arabinose as well as acetic acid which are present in ssl. the assimilation of hexoses was faster than pentoses during cultivation in a synthetic medium. however, all sugars were assimilated co ... | 2003 | 12618037 |
| regulation of expression of the rhizopus oryzae uricase and urease enzymes. | the regulation of intracellular urease and uricase activities was examined in rhizopus oryzae. urease activity (2.4 u/mg protein) was present in r. oryzae mycelium grown in minimal medium containing nh4ci as sole nitrogen source. this activity increased threefold under nitrogen derepression conditions, but no induction by urea was detected. control of urease activity in r. oryzae differs from that found in neurospora crassa but resembles the situation in aspergillus nidulans. no uricase activity ... | 2002 | 12619824 |
| in vitro evaluation of combination of terbinafine with itraconazole or amphotericin b against zygomycota. | the combined activity in vitro of amphotericin b/terbinafine and itraconazole/terbinafine was assessed against 17 clinical isolates of zygomycota using a checkerboard technique. itraconazole/terbinafine combination exhibited a potent synergistic effect against the most of strains. amphotericin b/terbinafine combination showed an indifferent interaction for rhizopus oryzae, and an additive effect for the other species. | 2003 | 12663161 |
| zygomycosis: a report of eleven cases and a review of the brazilian literature. | eleven cases of zygomycosis (mucormycosis) observed throughout an eighteen year period (1982-2000) have been reviewed. the most important demographic and clinical data of seven patients were tabulated. the remaining four are related as illustrative cases. seven patients presented with the pulmonary form of the disease; two patients presented with the pulmonary manifestation associated with sinusitis; and two patients presented with the rhinocerebral form. predisposing conditions, in decreasing o ... | 2002 | 12716233 |
| safety evaluation of lipase produced from rhizopus oryzae: summary of toxicological data. | the toxicity of lipase d, an enzyme preparation, was evaluated in a series of studies. lipase d selectively hydrolyzes triglycerides of fatty acids. it also catalyzes the interesterification of edible fats and oils. in a 13-week gavage study, sprague-dawley rats received lipase d at levels of 0, 500, 1000, or 2000 mg/kg body wt./day. a dose dependent decrease in urinary ph was observed, but there were no effects on electrolyte balance, kidney weight, or histology of the kidney. the no-observed-a ... | 2003 | 12726758 |
| endophytic fungi associated with mediterranean plants as a source of mycelium-bound lipases. | a screening of endophytic fungi isolated from mediterranean plants rendered a mycelium-bound lipase from a strain of rhizopus oryzae that catalyzed the esterification of fatty acids in isooctane. the influence of various factors (water content, temperature, and ph) on ester synthesis was investigated. catalytic activity was inversely correlated with water content. this enzyme was active over the entire ph range studied, from ph 3 to ph 8, and activity was maximal at ph 4 and ph 7. the enzyme was ... | 2003 | 12744662 |
| evaluation of the post-antifungal effect (pafe) of amphotericin b and nystatin against 30 zygomycetes using two different media. | the post-antifungal effect (pafe) of amphotericin b and nystatin against 30 clinical zygomycetes was evaluated using two different media. pafe is a suppression of fungal growth after limited drug exposure. the mics of both drugs were determined using nccls m38-p guidelines. a spectrophotometric method was used to determine pafe in vitro. spores were exposed to amphotericin b and nystatin in rpmi-1640 or am3 at concentrations of 4 x and 1 x mic for 4 h for absidia sp. and at 1 x and 0.5 x mic for ... | 2003 | 12775672 |
| induction of rhizopus oryzae pyruvate decarboxylase genes. | two pyruvate decarboxylase genes, pdca, and pdcb, were cloned from rhizopus oryzae. these genes are similar to each other with approximately 85% nucleotide sequence identity within the coding region. multiple transcriptional start sites and polyadenylation sites were found for both genes. the deduced translation product of each gene results in a 561 amino acid protein with approximate molecular weight of 61 kda each. the amino acid identity between the two proteins was 91% as calculated by lipma ... | 2003 | 12783195 |
| evaluation of two types of polyurethane for the immobilisation of rhizopus oryzae for copper uptake. | previous studies have shown that rhizopus oryzae strain im 057412 grown in reticulated polyurethane foam demonstrated the same heavy metal adsorption capacity as the free biomass. immobilisation in other types of polymers was shown to reduce the biomass uptake capacity because of mass transfer limitations due to the restricted porosity of the immobilisation matrices. for practical purposes the growing of biomass in polyurethene support particles to use as a commercial adsorbent is not viable or ... | 2003 | 12830953 |
| role of the pectinolytic enzyme in the lactic acid fermentation of potato pulp by rhizopus oryzae. | rhizopus oryzae strain nbrc 4707 produced lactic acid and ethanol more efficiently than strain nrrl 395 in potato pulp, an agricultural by-product of the starch industry. the two strains developed comparable activities of xylanase, cellulase, alpha-amylase, and glucoamylase, while the polygalacturonase activity of strain nbrc 4707 was double that of strain nrrl 395. the addition of commercial pectinase enhanced the formation of metabolites, suggesting that the degradation of pectic substances de ... | 2003 | 12844245 |
| cloning and characterisation of a glucoamylase gene (glam) from the dimorphic zygomycete mucor circinelloides. | this article reports a novel strategy for the cloning of glucoamylase genes using conserved sequences and semi-nested pcr and its application in cloning the glam glucoamylase gene and cdna from the dimorphic zygomycete mucor circinelloides. the deduced 609-amino-acid enzyme (including signal peptide) is 63% identical to the rhizopus oryzae raw starch-degrading glucoamylase and is the third glucoamylase reported to have the putative starch-binding domain placed n-terminally. the c-terminal cataly ... | 2003 | 12883866 |
| evaluation of the catalytic activity of lipases immobilized on chrysotile for esterification. | in the present work, the ester synthesis in organic media catalyzed by lipases immobilized on chrysotile was studied. lipases of different sources (mucor javanicus, pseudomonas cepacia, rhizopus oryzae, aspergillus niger and candida rugosa) were immobilized on chrysotile, an inexpensive magnesium silicate, and used for esterification of hexanoic, octanoic and lauric acid with methanol, ethanol, 1-butanol and 1-octanol at 25 c in hexane as solvent. the best results were obtained with mucor javani ... | 2003 | 12894300 |
| a new solution for an old problem: the regiochemical distribution of the acyl chains in galactolipids can be established by electrospray ionization tandem mass spectrometry. | electrospray ionization-quadrupole ion trap mass spectrometry (esi-qitms), either in positive- or in negative-ion mode, has been used to establish the chemical structures (chain length, degree of unsaturation, positional distribution) of the fatty acids attached to the primary (sn-1) and secondary (sn-2) hydroxyl groups of the glycerol moiety of natural monogalactosyl- (mgdg) and digalactosyldiacylglycerols (dgdg), isolated from the freshwater dinoflagellate glenodinium sanguineum and from a mar ... | 2003 | 12913862 |
| rdna its sequence of rhizopus oryzae: its application to classification and identification of lactic acid producers. | rhizopus oryzae is an important organism for its production of organic acids such as lactic acid, fumaric acid, etc. to date, there were no easy methods to classify strains according to their acid production. the sequences of the ribosomal rna-encoding dna (rdna) internal transcribed spacer (its) region of 64 strains of r. oryzae were analyzed and found to conserve mutations correspond to acid production. we have devised a way to use these mutations for a novel method to identify lactic-acid-pro ... | 2003 | 12951506 |
| antimicrobial activities of eusteralis deccanensis and e. quadrifolia essential oils. | antimicrobial activity of the essential oils of eusteralis deccanensis and e. quadrifolia were investigated on bacillus subtilis, b. megaterium, escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, rhizopus oryzae, aspergillus niger and colletotrichum musae. both the oils possess growth inhibitory activity against most of the microorganisms tested. | 2003 | 12974466 |
| the effects of metabolic alterations on experimental rhizopus oryzae (mucormycosis) infection. | 1956 | 13361273 | |
| the effects of cortisone and chemical inflammation on experimental mucormycosis (rhizopus oryzae infection). | 1957 | 13423366 | |
| leukopenia with granulocytopenia in experimental mucormycosis (rhizopus oryzae infection). | mucormycosis was produced in rabbits with sustained, severe leukopenia and granulocytopenia induced by repeated injections of nitrogen mustard. initially, these animals developed extensive fungus lesions at the site of inoculation which later became granulomatous and tended to heal. only the early phases of host resistance appeared impaired by the virtual elimination of the polymorphonuclear leukocyte as a factor in the host response. despite the persistent leukopenia and granulocytopenia, the l ... | 1957 | 13475609 |
| activation of quiescent mucormycotic granulomata in rabbits by induction of acute alloxan diabetes. | in normal rabbits subcutaneous granulomata produced by the injection of a spore suspension of rhizopus oryzae remained confined to the site of inoculation, showed no fungus proliferation, no longer yielded the agent on culture 10 weeks after inoculation, and eventually healed. similar well established granulomata in rabbits with acute alloxan diabetes induced 8, 10, and 15 days after injection of the fungus uniformly showed activation of the infection. this occurred only in animals showing aceto ... | 1958 | 13549649 |
| production of fumaric acid by rhizopus arrhizus. | 1959 | 13637887 | |
| studies of opportunistic fungi. i. inhibition of rhizopus oryzae by human serum. | 1961 | 13703046 | |
| gas exchange in rhizopus oryzae. | 1960 | 13826036 | |
| acute inflammation and tissue mast cells in adrenalectomized rats with cutaneous mucormycosis. | the effects of the absence of adrenal tissues on the inflammatory reaction and on the relationship of the tissue mast cells to the inflammatory process have been studied histologically in rats with cutaneous rhizopus oryzae infection. degranulation and regranulation of the tissue mast cells at the site of injury have been found to correlate respectively with the onset and subsidence of the exudative cellular phase of inflammation. in the adrenalectomized animals regranulation of these cells and ... | 1963 | 14074382 |
| on a new flavin mononucleotide produced by rhizopus oryzae. | 1963 | 14109857 | |
| the germination of sporangiospores of rhizopus arrhizus; spore swelling and germ-tube emergence. | 1964 | 14184103 | |
| [a case of mucormycosis (rhizopus oryzae)]. | 1964 | 14196841 | |
| cerebral mucormycosis: pathogenesis of the disease; description of the fungus, rhizopus oryzae, isolated from a fatal case. | 1955 | 14361464 | |
| fluorescent organophosphonates as inhibitors of microbial lipases. | short- and long-chain 1-o-alkyl-2-acylaminodeoxyglycero- and alkoxy-alkylphosphonic acid p-nitrophenyl esters were synthesized as inhibitors for analytical and mechanistic studies on lipolytic enzymes. the respective compounds contain perylene or nitrobenzoxadiazole as reporter fluorophores covalently bound to the omega-ends of the respective 2-acylamino- and alkoxy- residues. their inhibitory effects on the activities of three selected lipases showing different substrate preferences were determ ... | 2003 | 14499469 |
| liposomal amphotericin b, and not amphotericin b deoxycholate, improves survival of diabetic mice infected with rhizopus oryzae. | the efficacies of liposomal amphotericin b (lamb) and amphotericin b deoxycholate (amb) were compared in a diabetic murine model of hematogenously disseminated rhizopus oryzae infection. at 7.5 mg/kg of body weight twice a day (b.i.d.), lamb significantly improved overall survival compared to the rates of survival in both untreated control mice (p = 0.001) and mice treated with 0.5 mg of amb per kg b.i.d. (p = 0.047). these data indicate that high-dose lamb is more effective than amb in treating ... | 2003 | 14506054 |
| usefulness of frozen section in rhinocerebral mucormycosis diagnosis and management. | rhinocerebral mucormycosis (rcm) is a well-described fulminant fungal infection that presents acutely in patients with ketoacidosis and immunosuppression. very early diagnosis, established with the demonstration of characterised hyphae in tissues, greatly improves the prognosis of rcm. in this regard, the specificity and the sensitivity of frozen section for the diagnosis and the surgical debridement of rcm were evaluated in this study. | 2003 | 14506964 |
| comparison of fumaric acid production by rhizopus oryzae using different neutralizing agents. | fumaric acid fermentation in a 10-l bubble column fermenter using different neutralizing agents [caco(3), ca(oh)(2), nahco(3)] by rhizopus oryzae atcc 20344 was examined. it was found that in the fermentation using caco(3 )as the neutralizing agent the highest fumaric acid weight yield and volumetric productivity were obtained, 53.4% and 1.03 g/l x h(-1) respectively. in the nahco(3) case, the fumaric acid weight yield and volumetric productivity were 33.7% and 0.69 g/l x h(-1), respectively, mu ... | 2002 | 14508676 |
| use of turbidimetric growth curves for early determination of antifungal drug resistance of filamentous fungi. | a previously described microbroth kinetic system (j. meletiadis, j. f. meis, j. w. mouton, and p. e. verweij, j. clin. microbiol. 39:478-484, 2001) based on continuous monitoring of changes in the optical density of fungal growth was used to describe turbidimetric growth curves of different filamentous fungi in the presence of increasing concentrations of antifungal drugs. therefore, 24 clinical mold isolates, including rhizopus oryzae, aspergillus fumigatus, aspergillus flavus, and scedosporium ... | 2003 | 14532210 |
| genetic immobilization of proteins on the yeast cell surface. | a genetic system has been exploited to immobilize proteins in their active and functional forms on the cell surface of yeast, saccharomyces cerevisiae. dnas encoding proteins with a secretion signal peptide were fused with the genes encoding yeast agglutinins, a- and alpha-type proteins involved in mating. the fusion gene was introduced into s. cerevisiae and expressed under the control of several promoters. appearance of the fused proteins expressed on the cell surface was demonstrated biochemi ... | 2000 | 14538113 |
| activity of posaconazole in treatment of experimental disseminated zygomycosis. | three isolates of zygomycetes were used to produce a disseminated infection in nonimmunocompromised mice. against all zygomycete strains, amphotericin b significantly prolonged survival. itraconazole was inactive against rhizopus microsporus and rhizopus oryzae but was partially active against absidia corymbifera. posaconazole had no beneficial effects against r. oryzae but showed partial activity against a. corymbifera. posaconazole had a clear dose-response effect against r. microsporus. | 2003 | 14576138 |
| lactic acid production by rhizopus oryzae transformants with modified lactate dehydrogenase activity. | rhizopus oryzae is capable of producing high levels of lactic acid by the fermentation of glucose. yields typically vary over 60-80%, with the remaining glucose diverted primarily into ethanol fermentation. the goal of this work was to increase lactate dehydrogenase (ldh) activity, so lactic acid fermentation could more effectively compete for available pyruvate. three different constructs, pldha71x, pldha48xi, and pldha89vii, containing various lengths of the ldha gene fragment, were transforme ... | 2004 | 14624317 |
| effect of fungi fermentation on organoleptic properties, energy content and in-vitro multienzyme digestibility of cassava products (flour & gari). | the present study sought to investigate the effect of fungi fermentation on the energy content, sensory quality and the digestibility (in vitro) of cassava products (flour and gari). the fungi fermented cassava products (gari and flour) were produced, by fermenting cassava mash with pure strains of some common saprophytes, namely, aspergillus flavus, aspergillus niger, rhizopus oryzae and saccharomyces spp (baker's yeast and palm wine yeast) for 72 hrs before processing into cassava flour and ga ... | 2003 | 14653508 |
| enantioselective transesterification using lipase-displaying yeast whole-cell biocatalyst. | an enantioselective transesterification in non-aqueous organic solvent was developed by utilizing a lipase-displaying yeast whole cell biocatalyst constructed in our previous study. as a model reaction, optical resolution of (rs)-1-phenylethanol, which serves as one of chiral building blocks, was carried out by enantioselective transesterification with vinyl acetate. recombinant rhizopus oryzae lipase displayed on the yeast cell surface retained its activity in hexane, heptane, cyclohexane and o ... | 2004 | 14689244 |
| identification and characterization of a novel delta6-fatty acid desaturase gene from rhizopus arrhizus. | a cdna sequence putatively encoding a delta(6)-fatty acid desaturase was isolated from rhizopus arrhizus using reverse transcription polymerase chain reaction and rapid amplification of cdna ends methods. sequence analysis indicated that this cdna sequence had an open reading frame of 1377 bp encoding 458 amino acids of 52 kda. the deduced amino acid sequence showed high similarity to those of fungal delta(6)-fatty acid desaturases which comprised the characteristics of membrane-bound desaturase ... | 2004 | 14706830 |
| rhizopus arrhizus--a producer for simultaneous saccharification and fermentation of starch waste materials to l(+)-lactic acid. | rhizopus arrhizus, strain dar 36017, produced l(+)-lactic acid in a simultaneous saccharification and fermentation process using starch waste effluents. lactic acid at 19.5-44.3 g l(-1) with a yield of 0.85-0.96 g g(-1) was produced in 40 h using 20-60 g starch l(-1). supplementation of nitrogen source may be unnecessary if potato or corn starch waste effluent was used as a production medium. | 2003 | 14719810 |
| biosynthesis of tannase and gallic acid from tannin rich substrates by rhizopus oryzae and aspergillus foetidus. | modified solid-state fermentation (mssf) of tannin-rich substrates for production of tannase and gallic acid was carried out using two fungal cultures, rhizopus oryzae (ro iit rb-13, nrrl 21498) and aspergillus foetidus (gmrb013 mtcc 3557). the tannin rich substrates included powdered fruits of terminalia chebula and caesalpinia digyna pod cover powder. the different environmental parameters for the maximum production of tannase and gallic acid were optimized through media engineering. the highe ... | 2004 | 14768027 |
| bioconversion of waste office paper to l(+)-lactic acid by the filamentous fungus rhizopus oryzae. | l(+)-lactic acid production was investigated using an enzymatic hydrolysate of waste office automation (oa) paper in a culture of the filamentous fungus rhizopus oryzae. in 4 d culture, 82.8 g/l glucose, 7 g/l xylose, and 3.4 g/l cellobiose contained in the hydrolysate were consumed to produce 49.1 g/l of lactic acid. the lactic acid yield and production rate were only 0.59 g/g and 16.3 g/l/d, respectively, only 75% and 61% of the results from the glucose medium. the low production rate from was ... | 2004 | 14987724 |
| evidence for selective hydrolysis of aliphatic copolyesters induced by lipase catalysis. | high molar mass random poly(butylene succinate-co-butylene sebacate), p(bs-co-bse), and poly(butylene succinate-co-butylene adipate), p(bs-co-ba), with different composition, were synthesized and subjected to enzymatic hydrolysis by lipase from mucor miehei or from rhizopus arrhizus. the enzymatic hydrolysis of p(bs-co-bse)s and p(bs-co-ba)s films produced a mixture of water-soluble monomers and co-oligomers that were separated and identified by on-line high performance liquid chromatography/ele ... | 2004 | 15003003 |
| repair of plasmid dna used for transformation of rhizopus oryzae by gene conversion. | techniques for genetic manipulation of the filamentous fungus rhizopus have been hampered due to a lack of understanding regarding the recombination and replication mechanisms that affect the fate of introduced dna. the ability to target chromosomal integration of a plasmid has been difficult because dna transformed into rhizopus rarely integrates and is autonomously replicated in a high molecular weight concatenated arrangement (i.e., series or chain). linearization of the plasmid prior to tran ... | 2004 | 15007626 |
| investigation of the importance of the c-2 oxygen function in the transformation of stemodin analogues by rhizopus oryzae atcc 11145. | a new stemodinoside, stemodin-alpha-l-arabinofuranoside (5), was isolated from the plant stemodia maritima. incubation of stemodin (2) with rhizopus oryzae atcc 11145 gave 2 alpha,7 beta,13(s)-trihydroxystemodane (17) and 2 alpha,3 beta,13(s),16 alpha-tetrahydroxystemodane (18) whilst stemodinone (8) afforded 6 alpha,13(s)-dihydroxystemodan-2-one (19). the bioconversion of 2 beta,13(s)-dihydroxystemodane (10) by the fungus yielded 2 beta,7 beta,13(s)-trihydroxystemodane (20) whereas stemod-12-en ... | 2004 | 15016566 |
| expression of a rhizopus oryzae lipase in pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter. | a rhizopus oryzae lipase gene has been expressed in pichia pastoris as a reporter using the formaldehyde dehydrogenase 1 promoter (pfld1) of this organism, which has been reported to be strongly and independently induced by either methanol as sole carbon source or methylamine as sole nitrogen source. levels of lipase expressed and secreted under the control of the pfld1 at different induction conditions have been compared to those obtained with the commonly used alcohol oxidase 1 promoter (paox1 ... | 2004 | 15063618 |
| development of a system for integrative and stable transformation of the zygomycete rhizopus oryzae by agrobacterium-mediated dna transfer. | two transformation systems, based on the use of cacl(2)/peg and agrobacterium tumefaciens, respectively, were developed for the zygomycete rhizopus oryzae. irrespective of the selection marker used, a pyr4 marker derived from r. niveus or a dominant amds(+) marker from aspergillus nidulans, and irrespective of the configuration of the transforming dna (linear or circular), the transformants obtained with the cacl(2)/peg transformation method were found to carry multiple copies of tandemly linked ... | 2004 | 15067540 |
| complete resolution of pulmonary rhizopus oryzae infection with itraconazole treatment: more evidence of the utility of azoles for zygomycosis. | zygomycosis often requires aggressive surgical and antifungal therapy. we report a non-neutropenic patient with myelodysplastic syndrome and iron overload receiving cytotoxic therapy who presented with pulmonary rhizopus oryzae infection. this patient was cured through the use of itraconazole alone and the literature on the utility of azole antifungals for zygomycosis is reviewed. | 2004 | 15078434 |
| cloning and functional characterization of the rhizopus oryzae high affinity iron permease (rftr1) gene. | rhizopus oryzae is the most common etiologic agent of mucormycosis. clinical and animal model data clearly demonstrate that the presence of elevated available serum iron predisposes the host to develop mucormycosis. therefore, the high affinity iron permease (rftr1) which encodes a protein required to scavenge iron from the environment, is highly likely to be a critical determinant of virulence for r. oryzae. we have cloned rftr1 by using a pcr approach relying on degenerate primers designed fro ... | 2004 | 15158278 |
| purification of the extracellular pectinolytic enzyme from the fungus rhizopus oryzae nbrc 4707. | the pectinolytic enzyme from the solid-state culture of rhizopus oryzae nbrc 4707 was purified to homogeneity by column chromatography on cm-toyopearl 650 m and hydroxylapatite. the molecular weight of the enzyme was estimated by sds-polyacrylamide gel electrophoresis to be 31,000 and was reduced to 29,700 after treatment with endoglycosidase h. maximal activity was observed near ph 4.5 at 45 degrees c. the enzyme was shown to be endopolygalacturonase, as judged from the formation of oligogalact ... | 2004 | 15160610 |