Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| synthesis and structure-activity relationship study of monotesone-a, an antifungal component of monotes engleri. | the synthesis of (+/-)-monotesone-a, an antifungal component of monotes engleri, as well as its four structural analogues were accomplished starting from the corresponding mom-protected phloracetophenone and benzaldehyde derivatives. antifungal activities of the five flavanone derivatives were studied against candida albicans (14053 and atcc 10231), candida inconspicua, candida dubliniensis and candida krusei. | 2008 | 18404558 |
| [activity of anidulafungin against candida biofilms]. | currently, no standardized method to study the in vitro activity of antifungal agents on biofilms is available, thus, the comparison among different authors is difficult. the studies discussed in this review use the xtt reduction to measure the activity of antifungals on biofilms of 24 h of maturation. to date, biofilm anidulafungin mics of 47 isolates of candida spp. (25 candida albicans, 16 candida tropicalis, 5 candida dubliniensis and 1 candida parapsilosis) have been published. the geometri ... | 2008 | 18473507 |
| [prevalence of oral lesions by candida sp.: their varieties and serotypes in a population of patients with aids under a highly active antiretroviral therapy.]. | the aim of this study has been to determine the prevalence of oral candidiasis and oral candida carriers in an aids population under highly active antiretroviral therapy. eighty-six aids patients treated with an antiretroviral combination (indinavir o ritonavir o saquinavir + zidovudine [azt] + lamivudine [3tc]). patients were grouped attending the predisposing factors for hiv infection in: intravenous drug users (idu), heterosexuals, homosexuals, patients using hematological products or having ... | 1998 | 18473534 |
| candida dubliniensis and candida albicans display surface variations consistent with observed intergeneric coaggregation. | adherence of yeasts to other microorganisms and epithelial cell surfaces is important in their colonization. comparative studies based on the coaggregation of candida dubliniensis versus candida albicans with fusobacterium nucleatum and other oral bacteria suggested differences in the surfaces of these yeasts. transmission electron microscopy was used to test the hypothesis that there are morphologic variations in the cell surface of these two species. c. dubliniensis type strain cd36 and c. alb ... | 1999 | 18473545 |
| candida dubliniensis: an update. | 1999 | 18473572 | |
| differentiation of candida dubliniensis from candida albicans on rosemary extract agar and oregano extract agar. | candida dubliniensis is a recently described pathogenic species which shares many phenotypic features with candida albicans and therefore, may be misidentified in microbiological laboratories. because molecular methods can be onerous and unfeasible in routine mycological laboratories with restricted budgets such as those in developing countries, phenotypic techniques have been encouraged in the development of differential media for the presumptive identification of these species. we examined the ... | 2008 | 18484650 |
| candida dubliniensis: first identification in sfax hospital, tunisia. | candida dubliniensis, a newly described fungal pathogen associated mainly to immunocompromised host's infection, is phenotypically closely related to c. albicans. in this study, we report for the first time, isolation and identification, in tunisia, of 14 isolates of c. dubliniensis from 12 human immunodeficiency virus-negative patients hospitalised in the intensive care unit (icu) at habib bourguiba sfax hospital. our study was firstly based on the failure to grow at 45 degrees c. this presumpt ... | 2009 | 18522698 |
| molecular epidemiology of candidemia: evidence of clusters of smoldering nosocomial infections. | invasive fungal infections pose a serious threat to hospitalized patients worldwide. in particular, the prevalence of clusters of nosocomial infection among patients with candidemia remains unknown. the aim of this study was to investigate the molecular epidemiology of candidemia in a nationwide setting in iceland during a 16-year period. | 2008 | 18549311 |
| yeast diversity in the oral microbiota of subjects with periodontitis: candida albicans and candida dubliniensis colonize the periodontal pockets. | the term periodontitis encompasses several polymicrobial infectious diseases, of multifactorial etiology, with chronic and aggressive forms. in spite of the etiopathogenic differences between these two forms of the disease, few studies have analyzed the subgingival colonization by yeast. the objective of this investigation was to analyze the composition of the yeast microbiota present in the mucosa and subgingival sites of healthy individuals and patients with aggressive and chronic periodontiti ... | 2008 | 18608938 |
| isolation of candida dubliniensis for the first time in cali, colombia, and its identification with phenotyping methods. | candida dubliniensis is an emerging pathogenic yeast isolated mainly from the oral cavity of hiv-infected patients. the close phenotypic and genotypic relationship between c. albicans and c. dubliniensis has led to incorrectly identifying isolates of c. dubliniensis as c. albicans. the oral cavities of 107 diabetic patients were studied in cali, colombia, and 72 colonies of candida, with shades of green on chromagar candida culture media, were obtained. various phenotypic tests were carried out, ... | 2009 | 18622716 |
| the expression of genes involved in the ergosterol biosynthesis pathway in candida albicans and candida dubliniensis biofilms exposed to fluconazole. | the expression of the erg1, erg3, erg7, erg9, erg11 and erg25 genes in response to incubation with fluconazole and biofilm formation was investigated using reverse-transcription pcr and real-time pcr in candida albicans and candida dubliniensis clinical isolates. the viability of biofilm was measured using an 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2h-tetrazolium-5-carboxanilide (xtt) reduction assay and confocal scanning laser microscopy (cslm). expression of the erg11 gene was found to be low ... | 2009 | 18627475 |
| multiplex tandem pcr: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens. | we describe the first development and evaluation of a rapid multiplex tandem pcr (mt-pcr) assay for the detection and identification of fungi directly from blood culture specimens that have been flagged as positive. the assay uses a short-cycle multiplex amplification, followed by 12 simultaneous pcrs which target the fungal internal transcribed spacer 1 (its1) and its2 region, elongation factor 1-alpha (ef1-alpha), and beta-tubulin genes to identify 11 fungal pathogens: candida albicans, candid ... | 2008 | 18632914 |
| first molecular method for discriminating between candida africana, candida albicans, and candida dubliniensis by using hwp1 gene. | the authors report here a polymerase chain reaction-based assay using a single primer pair (cr-f/cr-r), allowing discrimination between candida africana and candida albicans by using the hwp1 gene. the method also identifies candida dubliniensis because it produces 3 different dna fragments: approximately 700 bp for c. africana, 941 bp for c. albicans, and 569 for c. dubliniensis. | 2008 | 18640803 |
| genetic diversity and exoenzyme activities of candida albicans and candida dubliniensis isolated from the oral cavity of brazilian periodontal patients. | mucosal surfaces are the primary oral reservoirs of candida species, but these species can also be found in subgingival biofilm. the present study investigated the genetic diversity and production of exoenzymes of c. albicans and c. dubliniensis isolated from the oral cavity of systemically healthy patients with periodontitis. | 2008 | 18672229 |
| correlation between enzyme production, germ tube formation and susceptibility to fluconazole in candida species isolated from patients with denture-related stomatitis and control individuals. | phospholipase and proteinase secretion in yeasts of the genus candida has been described as a relevant virulence factor. also, germ tube formation by candida albicans is associated with its invasive capacity and is considered an important pathogenic mechanism. | 2008 | 18764856 |
| variation of cell surface hydrophobicity and biofilm formation among genotypes of candida albicans and candida dubliniensis under antifungal treatment. | candida infections are frequently associated with formation of biofilms on artificial medical devices. this work studied variation of cell surface hydrophobicity (csh) and formation of biofilm in relation to candida albicans and candida dubliniensis genotypes and an effect of some conventional antifungal agents on both csh and biofilm. the 50 isolates of c. albicans and c. dubliniensis were classified into genotypes a, b, c, and d, genotype d being exclusively represented by c. dubliniensis. no ... | 2008 | 18772934 |
| gain-of-function mutations in the transcription factor mrr1 are responsible for overexpression of the mdr1 efflux pump in fluconazole-resistant candida dubliniensis strains. | candida dubliniensis, a yeast that is closely related to candida albicans, can rapidly develop resistance to the commonly used antifungal agent fluconazole in vitro and in vivo during antimycotic therapy. fluconazole resistance in c. dubliniensis is usually caused by constitutive overexpression of the mdr1 gene, which encodes a multidrug efflux pump of the major facilitator superfamily. the zinc cluster transcription factor mrr1p has recently been shown to control mdr1 expression in c. albicans ... | 2008 | 18809934 |
| isolation of candida dubliniensis in denture stomatitis. | to describe the isolation of candida dubliniensis from a patient with denture stomatitis and to compare with the presence of yeasts in the oral cavities of denture wearers. | 2009 | 18950745 |
| phenotypic and genotypic identification of candida dubliniensis from subgingival sites in immunocompetent subjects in argentina. | it is generally recognized that candida dubliniensis is commonly found in immunocompromised patients, such as those with advanced human immunodeficiency virus infection, at sites of periodontal disease. since there are no data available for argentina, the aim of this study was to determine the prevalence of and to identify c. dubliniensis in periodontal pockets from immunocompetent subjects living in buenos aires, argentina, through a comparison of phenotypic and molecular assays. | 2008 | 18954358 |
| arachidonic acid increases antifungal susceptibility of candida albicans and candida dubliniensis. | during candida albicans infection, arachidonic acid (aa) is released from phospholipids of infected host cell membranes and used by c. albicans as the sole carbon source and for production of eicosanoids. aa can be incorporated into the phospholipids of yeasts, influencing the saturation level and fluidity of yeast cell membranes. it is suggested that the effectiveness of polyene (e.g. amphotericin b) and imidazole (e.g. clotrimazole) antifungals may depend upon the level of unsaturation and erg ... | 2009 | 18971215 |
| neonatal septicaemia in a premature infant due to candida dubliniensis. | candida dubliniensis is a recently described species that shares many features with candida albicans. there are very few reports of isolation of this species from bloodstream in adults and paediatric population. here we report a case of neonatal septicaemia produced by c. dubliniensis in a premature infant admitted to neonatal intensive care unit. the preterm male neonate with a gestational age of 30 weeks and a birth weight of 1.2 kg presented with respiratory distress syndrome for which mechan ... | 2008 | 18974498 |
| molecular epidemiology of candida albicans and its closely related yeasts candida dubliniensis and candida africana. | we performed a molecular study to determine the occurrence of candida albicans, candida africana, and candida dubliniensis in different clinical samples. the study provides new insights into the epidemiology of candidiasis in hospitalized patients in three hospitals in southern italy. it also reports the first detailed epidemiological data concerning the occurrence of c. africana in clinical samples. | 2009 | 18987171 |
| in vitro activity of essential oils extracted from plants used as spices against fluconazole-resistant and fluconazole-susceptible candida spp. | in the present study, the antifungal activity of selected essential oils obtained from plants used as spices was evaluated against both fluconazole-resistant and fluconazole-susceptible candida spp. the candida species studied were candida albicans, candida dubliniensis, candida tropicalis, candida glabrata, and candida krusei. for comparison purposes, they were arranged in groups as c. albicans, c. dubliniensis, and candida non-albicans. the essential oils were obtained from cinnamomum zeylanic ... | 2008 | 18997851 |
| [candida dubliniensis: identification methods and epidemiologic implication.] | candida dubliniensis was recently described (1995) associated with oral candidiasis in hiv-positive patients. this organism is very closely related to the pathogenic human yeast, candida albicans, and share a great number of phenotypic and genotypic characters. this great similarity limits the discrimination between these two species. several phenotypic and molecular methods were developed. the phenotypic methods are simply used in routine discrimination between these two species and depend on t ... | 2008 | 19046828 |
| rapid evolution of cse4p-rich centromeric dna sequences in closely related pathogenic yeasts, candida albicans and candida dubliniensis. | the cse4p-containing centromere regions of candida albicans have unique and different dna sequences on each of the eight chromosomes. in a closely related yeast, c. dubliniensis, we have identified the centromeric histone, cdcse4p, and shown that it is localized at the kinetochore. we have identified putative centromeric regions, orthologous to the c. albicans centromeres, in each of the eight c. dubliniensis chromosomes by bioinformatic analysis. chromatin immunoprecipitation followed by pcr us ... | 2008 | 19060206 |
| differentiation of candida dubliniensis on chrom agar and pal's agar. | difference in expression of putative virulence factors and in antifungal susceptibility among different candida species has raised the need for species-level identification. the close relationship of candida dubliniensis with c. albicans has led to misidentification of c. dubliniensis isolates as c. albicans. phenotypic tests include ability to produce chlamydospore on casein agar, colony colour development on differential media chrom agar candida medium and ability to form hyphal fringe on pal' ... | 2009 | 19172062 |
| comparison of the susceptibilities of clinical isolates of candida albicans and candida dubliniensis to essential oils. | here, a microdilution technique based on the m27-a2 protocol (nccls, 2002) was employed to compare the susceptibilities of candida albicans and candida dubliniensis to essential oils extracted from plants used as spices. the chemical compositions of the essential oils were defined based on the analysis of retention indices obtained by gas chromatography-mass spectroscopy. taken together, the results showed that the activity of the compounds against the two species was similar. | 2010 | 19207850 |
| multifocal osteomyelitis caused by candida dubliniensis. | candida dubliniensis is an emerging fungal pathogen, especially in immunodeficient patients. we report what is to the best of our knowledge the first case of multifocal osteomyelitis following disseminated infection in a patient after haematopoietic stem cell transplantation. pfge for typing of c. dubliniensis was developed and the necessity of long-term antifungal therapy is discussed. | 2009 | 19208893 |
| [isolation of candida dubliniensis in different clinical samples. analysis of phenotypical methods to differentiate it from candida albicans]. | in order to estimate the frequence of candida dubliniensis in clinical samples in f. j. muñiz infectious diseases hospital, a total of 388 yeasts from september 2005 to august 2007. there were 212 isolates which presented a green color on chromagar candida medium and produced germ tubes and chlamidoconidiae in milk-agar; so as to distinguish whether they corresponded to candida albicans or c. dubliniensis, different phenotypical methods were utilized. it was also evaluated the usefulness of each ... | 2008 | 19213243 |
| hypertonic sabouraud dextrose agar as a substrate for differentiation of candida dubliniensis. | in this study, we aimed to detect the proportion of candida dubliniensis among yeast strains previously identified as c. albicans by using several phenotypic methods and pcr.for this purpose, we screened 300 strains by using phenotypic tests suggested for the identification of c. dubliniensis in the literature, but we detected high proportion of false-positive reactions. only two strains (0.6%) were detected as true c. dubliniensis by pcr and api id 32c methods. moreover, these two strains gave ... | 2009 | 19229655 |
| genome-wide gene expression profiling and a forward genetic screen show that differential expression of the sodium ion transporter ena21 contributes to the differential tolerance of candida albicans and candida dubliniensis to osmotic stress. | candida albicans is more pathogenic than candida dubliniensis. however, this disparity in virulence is surprising given the high level of sequence conservation and the wide range of phenotypic traits shared by these two species. increased sensitivity to environmental stresses has been suggested to be a possible contributory factor to the lower virulence of c. dubliniensis. in this study, we investigated, in the first comparison of c. albicans and c. dubliniensis by transcriptional profiling, glo ... | 2009 | 19239621 |
| the enigma of the major repeat sequence of candida albicans. | the major repeat sequence, discovered in the yeast candida albicans, is a stretch of repeated dna that occurs nine times in the haploid genome of this opportunistic fungal pathogen and probably a similar number of times in the genome of candida dubliniensis. in c. albicans it constitutes 1-2% of the genome. its occurrence is limited to those two species. despite its major role as a genomic feature, its function, mode of expansion in size due to duplication of internal subunits, and its origin an ... | 2009 | 19257844 |
| recognition of candida albicans als3 by the germ tube-specific monoclonal antibody 3d9.3. | monoclonal antibody 3d9.3 (mab 3d9.3) reacts with the surface of candida albicans germ tubes and recognizes a protein epitope. we used a two-step chromatography procedure to purify and identify the antigen (3d9) from c. albicans strain 66396 germ tubes. mab 3d9.3 recognized two intense protein bands at 140 and 180 kda. a comparative analysis between theoretical and experimental mass spectrum peaks showed that both bands corresponded to als3. this conclusion was supported by lack of reactivity be ... | 2009 | 19291169 |
| breakthrough candida dubliniensis fungemia in an acute myeloid leukemia patient during voriconazole therapy successfully treated with caspofungin. | 2009 | 19297283 | |
| molecular epidemiology of global candida dubliniensis isolates utilizing genomic-wide, co-dominant, pcr-based markers for strain delineation. | the molecular epidemiology of candida dubliniensis has been studied using large complex dna probes for southern analysis and has revealed the existence of distinct genotypes within this species. the aim of the present study was to utilize a pcr-based analysis of molecular co-dominant markers to assess the relatedness of a global and temporally diverse collection of well characterized isolates of c. dubliniensis. sixty-two c. dubliniensis strains were collected from the authors of previously publ ... | 2009 | 19353373 |
| [production of virulence factors in vitro by pathogenic species of the genus candida]. | the growth capacity at 39 degrees c and 42 degrees c, production of hydrolytic enzymes and hemolytic activity of 21 clinical and reference strains of seven species of candida spp were evaluated in vitro.candida dubliniensis and candida krusei demonstrated lower virulence potential and candida albicans higher potential. | 2009 | 19448950 |
| [activity of micafungin against candida biofilms]. | most recalcitrant infections are associated to colonization and microbial biofilm development. these biofilms are difficult to eliminate by the immune response mechanisms and the current antimicrobial therapy. | 2009 | 19463277 |
| colony morphologies, species, and biotypes of yeasts from thrush and denture stomatitis. | objective. to study the species and phenotypic characteristics of yeasts, i.e. colony morphology, biotypes, and biotype relatedness, and the oral distribution of yeasts, in thrush and denture stomatitis. material and methods. yeast colony morphology was observed under a stereo-microscope and photographed with a digital camera. genus, species, and biotypes of the yeast isolates were identified by using a commercial kit, id 32c. yeast biotype dendrograms were generated by spotfire software and sps ... | 2009 | 19479451 |
| isolation of candida dubliniensis from denture wearers. | candida albicans is considered the most important candida species able to cause oral infections in denture wearers. in recent years, candida dubliniensis has emerged as a pathogenic yeast in humans. the close phenotypic similarities of c. albicans and c. dubliniensis have led to the misidentification of these species. in this work, our aim was to verify through pcr the presence of c. dubliniensis in palate and maxillary denture samples from 112 denture wearers presenting with or without denture- ... | 2009 | 19498202 |
| purification and germination of candida albicans and candida dubliniensis chlamydospores cultured in liquid media. | candida albicans and candida dubliniensis are the only candida sp. that have been observed to produce chlamydospores. the function of these large, thick-walled cells is currently unknown. in this report, we describe the production and purification of chlamydospores from these species in defined liquid media. staining with the fluorescent dye fun-1 indicated that chlamydospores are metabolically active cells, but that metabolic activity is undetectable in chlamydospores that are >30 days old. how ... | 2009 | 19538507 |
| differential expression of candida dubliniensis-secreted aspartyl proteinase genes (cdsap1-4) under different physiological conditions and during infection of a keratinocyte culture. | the in vitro and keratinocyte (hacat cells) culture expression of four putative genes coding for secreted aspartyl proteases of candida dubliniensis-cdsap1, cdsap2, cdsap3, and cdsap4 (cdsap1-4) - is reported for the first time. in addition, cdsap7, 8, 9, and 10, orthologous genes of candida albicans, were recognized in c. dubliniensis genome. there are no orthologs of c. albicans sap5 and 6 in c. dubliniensis. the expression of cdsap1 and 2 was independent of the morphological stage of c. dubli ... | 2009 | 19538512 |
| phylogeny and evolution of the aspartyl protease family from clinically relevant candida species. | aspartyl proteases are a class of enzymes that include the yeast aspartyl proteases and secreted aspartyl protease (sap) superfamilies. several sap superfamily members have been demonstrated or suggested as virulence factors in opportunistic pathogens of the genus candida. candida albicans, candida tropicalis, candida dubliniensis and candida parapsilosis harbour 10, four, eight and three sap genes, respectively. in this work, genome mining and phylogenetic analyses revealed the presence of new ... | 2009 | 19547880 |
| oral distribution of candida species and presence of oral lesions in brazilian leprosy patients under multidrug therapy. | the aim of this study was to evaluate the prevalence of candida spp. and presence of oral lesions in brazilian leprosy patients under multidrug therapy (mdt). | 2009 | 19549111 |
| the importance of strain variation in virulence of candida dubliniensis and candida albicans: results of a blinded histopathological study of invasive candidiasis. | the pathogenic yeast candida dubliniensis is increasingly reported as a cause of systemic fungal infections. we compared the virulence of 9 clinical bloodstream isolates of c. dubliniensis with 3 c. albicans isolates in a murine model of invasive candidiasis. quantification of organisms and inflammatory changes in kidneys of infected animals were evaluated in a blinded, systematic manner. average 7-day mortality among animals infected with c. dubliniensis was 21.0% (33/157 animals; range for str ... | 2009 | 19604278 |
| evaluation of chrom-pal medium for the isolation and direct identification of candida dubliniensis in primary cultures from the oral cavity. | candida albicans is the species most frequently isolated from oral specimens, but the recovery of other candida species such as candida dubliniensis is increasing. differentiation of c. dubliniensis from c. albicans requires special tests and both species are misidentified in some studies. chrom-pal (ch-p) is a novel chromogenic medium used in our laboratory for differentiation between c. albicans and c. dubliniensis on the basis of colony colour and morphology, and chlamydospore production. the ... | 2009 | 19608690 |
| candidaemia with uncommon candida species: predisposing factors, outcome, antifungal susceptibility, and implications for management. | the risk factors for and clinical features of bloodstream infection with uncommon candida spp. (species other than c. albicans, c. glabrata, c. parapsilosis, c. tropicals and c. krusei) are incompletely defined. to identify clinical variables associated with these species that might guide management, 57 cases of candidaemia resulting from uncommon candida spp. were analysed in comparison with 517 episodes of candida albicans candidaemia (2001-2004). infection with uncommon candida spp. (5.3% of ... | 2009 | 19614718 |
| adherence, colonization and dissemination of candida dubliniensis and other candida species. | adherence to host cells is essential for yeasts to develop their full pathogenic potential since it triggers the process that leads to colonization and enables their persistence in the host. the aim of this work was to study the in vitro adherence of candida dubliniensis and other candida species, as well as the relation of adherence with the colonization and dissemination of these yeasts in an experimental mice model. clinical isolates of candida dubliniensis, candida albicans, candida glabrata ... | 2010 | 19626546 |
| monoclonal antibody-mediated inhibition of adhesion of candida albicans and candida dubliniensis to human epithelial cells. | the aim of this study was to assess the role of whole saliva, four saliva-derived preparations, and six monoclonal antibodies (mabs), directed against components of the cell wall of candida albicans, on the adhesion of c. albicans and candida dubliniensis to human epithelial cells (hec). c. albicans serotype a ncpf 3153 and c. albicans serotype b atcc 90028 showed higher adhesion to hec than c. dubliniensis ncpf 3949. pooled whole saliva was more efficient than salivary secretory immunoglobulin ... | 2009 | 19627362 |
| escherichia coli and its lipopolysaccharide modulate in vitro candida biofilm formation. | demystification of microbial behaviour in mixed biofilms could have a major impact on our understanding of infectious diseases. the objectives of this study were to evaluate in vitro the interactions of six different candida species and a gram-negative coliform, escherichia coli, in dual-species biofilms, and to assess the effect of e. coli lps on candida biofilm formation. a single isolate of e. coli atcc 25922 and six different species of candida, candida albicans atcc 90028, candida glabrata ... | 2009 | 19661208 |
| yeasts isolated from a fungus-growing ant nest, including the description of trichosporon chiarellii sp. nov., an anamorphic basidiomycetous yeast. | thirty-nine yeast strains were recovered from a field nest of a primitive and putative novel attine ant species in the genus myrmicocrypta (hymenoptera: formicidae: tribe attini). yeasts isolated from the fungus garden and waste deposit included candida dubliniensis, candida oleophila, cryptococcus haglerorum and hanseniaspora uvarum. in addition, one morphological type was isolated overwhelmingly. sequencing data of partial large-subunit (lsu) rdna and the internal transcribed spacer (its) regi ... | 2010 | 19671730 |
| candida albicans or candida dubliniensis? | candida albicans is increasing as an opportunistic pathogen causing candidemia and candidiasis worldwide. in addition, other non-albicans candida species are now also associated with pertinent infections. these include the closely related c. dubliniensis, which shares many phenotypic similarities with c. albicans. these similarities pose problems in the identification of isolates and have previously led to misidentification of these species. as a result, several identification techniques based o ... | 2011 | 19682314 |
| a ser29leu substitution in the cytosine deaminase fca1p is responsible for clade-specific flucytosine resistance in candida dubliniensis. | the population structure of the opportunistic yeast pathogen candida dubliniensis is composed of three main multilocus sequence typing clades (clades c1 to c3), and clade c3 predominantly consists of isolates from the middle east that exhibit high-level resistance (mic(50) > or = 128 microg/ml) to the fungicidal agent flucytosine (5fc). the close relative of c. dubliniensis, c. albicans, also exhibits clade-specific resistance to 5fc, and resistance is most commonly mediated by an arg101cys subs ... | 2009 | 19704126 |
| comparative genomics of the fungal pathogens candida dubliniensis and candida albicans. | candida dubliniensis is the closest known relative of candida albicans, the most pathogenic yeast species in humans. however, despite both species sharing many phenotypic characteristics, including the ability to form true hyphae, c. dubliniensis is a significantly less virulent and less versatile pathogen. therefore, to identify c. albicans-specific genes that may be responsible for an increased capacity to cause disease, we have sequenced the c. dubliniensis genome and compared it with the kno ... | 2009 | 19745113 |
| in vitro efficacy of amphotericin b, 5-fluorocytosine, fluconazole, voriconazole and posaconazole against candida dubliniensis isolates using time-kill methodology. | candida dubliniensis is a recently described yeast that causes infections in mucosal surfaces as well as sterile body sites. candida dubliniensis develops resistance to fluconazole (flc) more rapidly than the closely related species c. albicans. the killing activity of amphotericin b (amb), 5-fluorocytosine (5fc), flc, voriconazole (vrc) and posaconazole (pos) was determined against six c. dubliniensis clinical isolates, identified using molecular biological methods and c. dubliniensis cd36 refe ... | 2010 | 19761489 |
| paradoxical growth of candida dubliniensis does not preclude in vivo response to echinocandin therapy. | candida dubliniensis commonly shows paradoxical or trailing growth effects in vitro in the presence of echinocandins. we tested the in vitro activities of anidulafungin, caspofungin, and micafungin against clinical isolates of c. dubliniensis and evaluated the efficacy of these drugs in two murine models of systemic infection. the three echinocandins were similarly effective in the treatment of experimental disseminated infections with c. dubliniensis strains showing or not showing abnormal grow ... | 2009 | 19786599 |
| genetic differences between avian and human isolates of candida dubliniensis. | when candida dubliniensis isolates obtained from seabird excrement and from humans in ireland were compared by using multilocus sequence typing, 13 of 14 avian isolates were genetically distinct from human isolates. the remaining avian isolate was indistinguishable from a human isolate, suggesting that transmission may occur between humans and birds. | 2009 | 19788816 |
| detection and antifungal susceptibility testing of oral candida dubliniensis from human immunodeficiency virus-infected patients. | candida dubliniensis, an opportunistic yeast that has been implicated in oropharyngeal candidiasis (opc) in patients infected with human immunodeficiency virus (hiv) may be under-reported due to its similarity with candida albicans. resistance to fluconazole is often seen in c. dubliniensis isolates from clinical specimens. | 2009 | 19805956 |
| real-time lightcycler polymerase chain reaction and melting temperature analysis for identification of clinically important candida spp. | invasive candidiasis is a major fungal infection occurring in patients who have prolonged hospital admissions. the rapid detection and confirmation of candida spp. in clinical specimens is essential for efficient management and improved prognosis of these patients. the purpose of this study was to develop a real-time lightcycler polymerase chain reaction (pcr) assay for the identification of candida spp. commonly associated with invasive infections. | 2009 | 19949751 |
| prevalence of candida dubliniensis among cancer patients in kuwait: a 5-year retrospective study. | summary despite close genetic and phenotypic relationship of candida dubliniensis with candida albicans, its role in human disease is mostly restricted to oral colonisation, particularly among hiv-infected patients. the prevalence of c. dubliniensis in association with other disease conditions has been infrequently reported. in this study, we present data on the prevalence of c. dubliniensis among yeast species isolated from cancer patients over a 5-year period. a total of 1445 yeast isolates re ... | 2009 | 20002881 |
| comparative transcript profiling of candida albicans and candida dubliniensis identifies sfl2, a c. albicans gene required for virulence in a reconstituted epithelial infection model. | candida albicans and candida dubliniensis are closely related species displaying differences in virulence and genome content, therefore providing potential opportunities to identify novel c. albicans virulence genes. c. albicans gene arrays were used for comparative analysis of global gene expression in the two species in reconstituted human oral epithelium (rhe). c. albicans (sc5314) showed upregulation of hypha-specific and virulence genes within 30 min postinoculation, coinciding with rapid i ... | 2010 | 20023067 |
| phospholipase and proteinase activities of candida isolates from denture wearers. | summary the aim of the present study was to characterise phospholipase and proteinase activities of oral candida isolates from 100 denture wearers and to study the relationship of these activities with denture stomatitis. of 100 patients studied, 44 suffered from denture stomatitis. specimens were collected by swabbing the denture and underlying mucosa. isolates were previously identified by conventional mycological and genotypic methods. the phospholipase and proteinase activities were evaluate ... | 2009 | 20028461 |
| species identification of candida isolates obtained from oral lesions of hiv infected patients. | a total of 60 patients suspected to have aids with oral lesions suggestive of oral candidiasis were studied. candida species were isolated from 50 patients. candida albicans was the commonest isolate (70 %) followed candida parapsilosis(15%), candida glabrata (7.5%) and candida tropicalis (5%) respectively. candida dubliniensis was isolated from a single case only. though the reports from developed countries show more prevalence of the novel species candida dubliniensis, in our study it was isol ... | 2009 | 20101347 |
| extracts from alternanthera maritima as natural photosensitizers in photodynamic antimicrobial chemotherapy (pact). | this study investigated the effect of photodynamic antimicrobial chemotherapy (pact) using extracts from alternanthera maritima on the viability of candida dubliniensis. human infections constitute a great health problem. several antifungal drugs are currently available, but their uses are limited by a number of factors, such as low potency, poor solubility, microbial resistance, and drug toxicity. therefore, the search for new and more effective antimicrobial agents and the development of alter ... | 2010 | 20172737 |
| longitudinal genotyping of candida dubliniensis isolates reveals strain maintenance, microevolution, and the emergence of itraconazole resistance. | we investigated the population structure of 208 candida dubliniensis isolates obtained from 29 patients (25 human immunodeficiency virus [hiv] positive and 4 hiv negative) as part of a longitudinal study. the isolates were identified as c. dubliniensis by arbitrarily primed pcr (ap-pcr) and then genotyped using the cd25 probe specific for c. dubliniensis. the majority of the isolates (55 of 58) were unique to individual patients, and more than one genotype was recovered from 15 of 29 patients. a ... | 2010 | 20200288 |
| adherence ability of candida africana: a comparative study with candida albicans and candida dubliniensis. | summary in this study, we compared the adherence ability to human hela cells and biofilm formation of three closely related candida yeast. in our experiments, candida africana showed poor adhesion ability to human hela cells and the absence of biofilm formation on polyvinyl chloride strips. conversely, candida albicans and candida dubliniensis formed mature biofilms and stable attachment to hela cells. to our knowledge, this is the first comparative study reporting data on biofilm formation and ... | 2010 | 20202113 |
| response to oxidative stress in eight pathogenic yeast species of the genus candida. | in the course of an infection, the formation of reactive oxygen species by phagocytes and the antioxidant defense mechanisms of microorganisms play a crucial role in pathogenesis. in this study, isolates representing 8 pathogenic candida species-candida albicans, candida dubliniensis, candida famata, candida glabrata, candida guilliermondii, candida krusei, candida parapsilosis and candida tropicalis-were compared with regard to their resistance to oxidative stress in vitro. we evaluated degree ... | 2010 | 20229037 |
| experimental pathogenicity of candida albicans and candida dubliniensis with continuous and discontinuous fringes morphotypes. | summary a possible correlation between the presence of discontinuous fringes and high virulence has been previously suggested. the aim of this study was to compare the pathogenicity of candida albicans and candida dubliniensis with continuous and discontinuous fringes morphotypes on mice. for c. albicans, two discontinuous fringe morphotype isolates (pn 69, pn 74), two continuous fringe morphotype isolates (n 60, n 33) and one reference strain were used. for c. dubliniensis, three discontinuous ... | 2010 | 20337935 |
| a real-time pcr assay for the differentiation of candida species. | we established a real-time pcr assay for the detection and strain identification of candida species and demonstrated the ability to differentiate between candida albicans the most common species, and also candida parapsilosis, candida glabrata, candida tropicalis and candida dubliniensis by lightcycler pcr and melting curve analysis. | 2010 | 20456528 |
| comparison of phenotypic methods for the identification of candida dubliniensis. | mixed infections caused by different candida species are the rule rather than the exception. the discrimination between the two closely related species candida albicans and candida dubliniensis is not trivial. therefore, there is a need for fast, reliable, and inexpensive methods with high specificity for the identification and differentiation of these two candida species, which are frequently detected in the oral cavities of patients with a human immunodeficiency virus infection. | 2010 | 20457432 |
| analysis of gene evolution and metabolic pathways using the candida gene order browser. | candida species are the most common cause of opportunistic fungal infection worldwide. recent sequencing efforts have provided a wealth of candida genomic data. we have developed the candida gene order browser (cgob), an online tool that aids comparative syntenic analyses of candida species. cgob incorporates all available candida clade genome sequences including two candida albicans isolates (sc5314 and wo-1) and 8 closely related species (candida dubliniensis, candida tropicalis, candida parap ... | 2010 | 20459735 |
| candida dubliniensis does not show phospholipase activity: true or false? | the phospholipase activity in candida albicans and candida dubliniensis isolated from oral candidiasis cases were studied. | 2010 | 20464155 |
| candida species extracellular alcohols: production and effect in sessile cells. | cell-cell signaling alcohol molecules were recently identified in candida albicans and candida dubliniensis supernatants. to date, it is not known whether these molecules are produced by other candida species and their role in biofilm formation is not fully clarified. herein, candida parapsilosis and candida tropicalis extracellular alcohols production by planktonic cultures was analyzed by headspace-solid-phase microextraction and gas chromatography-mass spectrometry. both these candida species ... | 2010 | 20473968 |
| candida dubliniensis screening using the germ tube test in clinical yeast isolates and prevalence of c. dubliniensis in korea. | the aim of this study was to screen for c. dubliniensis using the germ tube test with human pooled serum (hps) in clinical isolates and investigate the prevalence of c. dubliniensis in korea. among 1,854 yeast strains isolated, 1,404 strains of c. albicans (on the basis of positive results of the germ tube test) and 192 germ tube-negative yeast strains were examined. all 1,596 clinical isolates were examined using the germ tube test with hps, the differential temperature, and nacl tolerance test ... | 2010 | 20486193 |
| candida dubliniensis: epidemiology and phenotypic methods for identification. | candida dubliniensis is an emerging pathogen first described in 1995, which shares many phenotypic features with candida albicans and therefore may be misidentified in microbial laboratories. despite various phenotypic techniques described in the literature to differentiate the two species, the correct identification of c. dubliniensis remains problematic due to phenotypic similarities between these species. thus, as the differences between both are best characterized at genetic levels, several ... | 2010 | 20490751 |
| drbc agar: a new tool for candida dubliniensis identification. | candida dubliniensis pathogenic species, which shares many phenotypic features with c. albicans, may be misidentified in the microbiology laboratory. the growth on drbc agar at 25 degrees c was shown to be a new tool for differentiation between c. dubliniensis and c. albicans. all 27 isolates of c. dubliniensis showed in this medium rough colonies (peripheral hyphal fringes) and abundant chlamydospore production, while all 103 isolates of c. albicans showed smooth colonies without fringes or chl ... | 2010 | 20490758 |
| experimental systemic virulence of oral candida dubliniensis isolates in comparison with candida albicans, candida tropicalis and candida krusei. | there are no previous studies on the comparative virulence of candida dubliniensis with other non-albicans species. the aim of this study was to compare the virulence and infection kinetics of c. dubliniensis and other species. candida albicans, c. dubliniensis, candida tropicalis and candida krusei (reference strains) were inoculated intravenously in mice. for infection kinetics evaluation, a group of five animals were sacrificed after 6 h, 3, 7, 14 and 21 days. microbiological evaluations (liv ... | 2010 | 20492535 |
| mechanisms of antifungal drug resistance in candida dubliniensis. | candida dubliniensis was first described in 1995 and is the most closely related species to the predominant human fungal pathogen candida albicans. c. dubliniensis is significantly less prevalent and less pathogenic than c. albicans and is primarily associated with infections in hiv-infected individuals and other immunocompromised cohorts. the population structure of c. dubliniensis consists of three well-defined major clades and is significantly less diverse than c. albicans. the majority of c. ... | 2010 | 20521937 |
| the effect of brief exposure to sub-therapeutic concentrations of chlorhexidine gluconate on germ tube formation of oral candida dubliniensis. | summary adherence of candida has been implicated as the first step in the pathogenesis of oral candidosis, and germ tube formation, a contributory attribute. while chlorhexidine gluconate is by far the most common antiseptic mouthwash prescribed in dentistry, its intraoral concentration fluctuates considerably because of the dynamics of the oral cavity. hence, the main objective of this study was to investigate the effect of brief exposure to three different sub-therapeutic concentrations of chl ... | 2010 | 20557460 |
| bacterial lipopolysaccharides variably modulate in vitro biofilm formation of candida species. | the objective of this study was to evaluate the effect of the bacterial endotoxin lps on candida biofilm formation in vitro. the effect of the lps of pseudomonas aeruginosa, klebsiella pneumoniae, serratia marcescens and salmonella typhimurium on six different species of candida, comprising candida albicans atcc 90028, candida glabrata atcc 90030, candida krusei atcc 6258, candida tropicalis atcc 13803, candida parapsilosis atcc 22019 and candida dubliniensis mya 646, was studied using a standar ... | 2010 | 20576747 |
| pphy--a cell-bound phytase from the yeast pichia anomala: molecular cloning of the gene pphy and characterization of the recombinant enzyme. | the pichia anomala gene pphy, which codes for a cell-bound phytase, was isolated from genomic dna by pcr, using oligonucleotide sequences derived from the n-terminal region of the purified phytase protein (pphyp) and a degenerate primer derived from conserved sequences of yeast and fungal phytases as primers. the gene harbours an orf of 1389bp, encoding a 462-amino-acid protein. the deduced amino acid sequence has similarity, to a varied extent, with those of phosphatases from pichia stipitis (6 ... | 2010 | 20599570 |
| differentiation of candida dubliniensis from candida albicans with the use of killer toxins. | the aim of this study was to report the ability of killer toxins, previously used as biotyping techniques, as a new tool to differentiate c. albicans from c. dubliniensis. the susceptibility of c. albicans and c. dubliniensis to killer toxins ranged from 33.9 to 93.3% and from 6.67 to 93.3%, respectively. | 2010 | 20602027 |
| prevalence and antifungal drug sensitivity of non-albicans candida in oral rinse samples of self-caring elderly. | gerodontology 2010; doi: 10.1111/j.1741-2358.2010.00407.x prevalence and antifungal drug sensitivity of non-albicans candida in oral rinse samples of self-caring elderly aim: to assess the prevalence and antifungal drug sensitivity of non-albicans candida (nac) species in elderly outpatients. materials and methods: we investigated oral rinse samples of 194 self-caring elderly population (mean age 83 years) with emphasis on background factors for harbouring nac. susceptibility of candida species ... | 2010 | 20609005 |
| phenotypic and genotypic characterisation of candida dubliniensis isolate in a patient with disseminated candidiasis. | 2010 | 20637055 | |
| differential filamentation of candida albicans and candida dubliniensis is governed by nutrient regulation of ume6 expression. | candida dubliniensis is closely related to candida albicans; however, it is responsible for fewer infections in humans and is less virulent in animal models of infection. c. dubliniensis forms fewer hyphae in vivo, and this may contribute to its reduced virulence. in this study we show that, unlike c. albicans, c. dubliniensis fails to form hyphae in yeast extract-peptone-dextrose (ypd) medium supplemented with 10% (vol/vol) fetal calf serum (ypds medium). however, c. dubliniensis filaments in w ... | 2010 | 20639413 |
| giant mycotic right atrial thrombus due to candida dubliniensis septicemia in a premature infant. | 2010 | 20661110 | |
| candida africana and its closest relatives. | summary candida africana is a recently described opportunistic yeast pathogen that has been linked to vaginal candidiasis. this yeast was first described, in 1995, as atypical chlamydospore-negative candida albicans strain, and subsequently proposed as a new candida species on the basis of morphological, biochemical and physiological characteristics clearly different from those of typical c. albicans isolates. phylogenetic studies based on the comparison of ribosomal dna sequences demonstrated t ... | 2010 | 20667001 |
| effect of inhibitors of arachidonic acid metabolism on prostaglandin e₂ production by candida albicans and candida dubliniensis biofilms. | arachidonic acid (aa) is released from infected host cells during candida albicans infection and may serve as carbon source for yeast growth and as precursor for the production of biologically active eicosanoids, such as prostaglandin e₂ (pge₂) by c. albicans. however, the mechanism involved in this production is still unclear. therefore, it was of interest to investigate the effect of different arachidonic acid metabolism inhibitors on pge₂ production by biofilms of c. albicans and the closely ... | 2011 | 20821232 |
| in vitro activity of fluconazole and voriconazole against clinical isolates of candida spp. by e-test method. | the in vitro susceptibility of clinical candida isolates towards fluconazole and voriconazole was determined using the e-test method. a total of 41 clinical isolates recovered from patients since 2004 until 2009 from two local hospitals in kuala lumpur, malaysia were used. these comprised candida tropicalis, candida albicans, candida krusei, candida parapsilosis, candida rugosa, candida dubliniensis and candida glabrata. strains from american type culture collection were used as quality control. ... | 2010 | 20962716 |
| recovery of candida dubliniensis and other candida species from the oral cavity of subjects with periodontitis who had well-controlled and poorly controlled type 2 diabetes: a pilot study. | the goal of this study was to determine the prevalence of candida dubliniensis and other candida species from saliva samples and from subgingival plaque samples at periodontally healthy and periodontally diseased sites in subjects who had type 2 diabetes and periodontitis. saliva and subgingival samples were obtained from 30 subjects with periodontitis: 15 with poorly controlled and 15 with well-controlled type 2 diabetes. samples were analyzed for the presence of c. dubliniensis and other candi ... | 2010 | 21044102 |
| effect of marine polyunsaturated fatty acids on biofilm formation of candida albicans and candida dubliniensis. | the effect of marine polyunsaturated fatty acids on biofilm formation by the human pathogens candida albicans and candida dubliniensis was investigated. it was found that stearidonic acid (18:4 n-3), eicosapentaenoic acid (20:5 n-3), docosapentaenoic acid (22:5 n-3) and docosahexaenoic acid (22:6 n-3) have an inhibitory effect on mitochondrial metabolism of both c. albicans and c. dubliniensis and that the production of biofilm biomass by c. dubliniensis was more susceptible to these fatty acids ... | 2010 | 21116408 |
| improved dna-fish for cytometric detection of candida spp. | aims: we developed improved methods for dna-based fluorescence in situ hybridization (fish) for rapid detection of candida spp. and candida albicans via flow cytometry. methods and results: two previously reported c. albicans-targeted dna probes were evaluated against whole cells of c. albicans and related candida species using a rapid, high-temperature hybridization protocol. one probe (calb2208) was shown for the first time to be suitable as a fish probe. although cell labelling for both pro ... | 2011 | 21205104 |
| a comparison of the antifungal activity of herbal toothpastes against other brands of toothpastes on clinical isolates of candida albicans and candida dubliniensis. | to evaluate the anti-candida activity on candida albicans and candida dubliniensis species of 2 herbal and 7 other brands of toothpastes commonly used in kuwait. | 2011 | 21252563 |
| differential virulence of candida albicans and c. dubliniensis: a role for tor1 kinase? | candida albicans and candida dubliniensis are two very closely related species of pathogenic yeast. c. albicans is the most prevalent species in the human gastrointestinal tract and is responsible for far more opportunistic infections in comparison with c. dubliniensis. this disparity is likely to be due to the reduced ability of c. dubliniensis to undergo the yeast to hypha transition, a change in morphology that plays an important role in c. albicans virulence. we have recently shown that hyph ... | 2011 | 21289475 |
| [prevalence of candida dubliniensis in a tertiary hospital in madrid]. | 2011 | 21333396 | |
| lack of candida africana and candida dubliniensis in vaginal candida albicans isolates in turkey using hwp1 gene polymorphisms. | candida africana differs from the common strains of c. albicans and c. dubliniensis morphologically, physiologically, genetically, and, in particular, clinically. this fungal pathogen is primarily recovered from genital specimens, especially in vaginal specimens. in this investigation, we reexamined 195 vaginal c. albicans isolates for the presence of c. africana and c. dubliniensis by using hyphal wall protein 1 (hwp1) gene polymorphisms. all study isolates were confirmed to be c. albicans, and ... | 2011 | 21380767 |
| application of hypertonic sabouraud glucose agar for differentiation of candida dubliniensis from candida albicans. | an evaluation of hypertonic sabouraud glucose agar (sga) with 6.5% nacl for phenotypic differentiation of candida dubliniensis from candida albicans is reported. identity of the test fungi (c. albicans, 84; c. dubliniensis, 18) was based on their typical phenotypic characteristics and confirmed by a diagnostic polymerase chain reaction that targets the novel c. dubliniensis group i intron in the large ribosomal subunit. at 96 h of incubation at 28 -¦c, all of the 84 c. albicans isolates showed g ... | 2011 | 21396542 |
| presence of candida spp. in the oral cavity of heart transplantation patients. | candida spp. can lead to infections or even fungal sepsis particularly among immunocompromised individuals. | 2011 | 21437462 |
| prevalence of candida dubliniensis among oral candida isolates in patients attending the kuwait university dental clinic. | the aim of this study was to determine the oral candidal carriage of patients seeking dental treatment at the kuwait university dental clinic and to ascertain the candida species composition among them. | 2011 | 21454999 |
| genetic relatedness of subgingival and buccal candida dubliniensis isolates in immunocompetent subjects assessed by rapd-pcr. | it is recognized that candida dubliniensis commonly colonizes oral and subgingival sites in immunocompetent subjects with periodontal disease. | 2009 | 21523209 |
| calcineurin controls drug tolerance, hyphal growth, and virulence in candida dubliniensis. | candida dubliniensis is an emerging pathogenic yeast species closely related to candida albicans and frequently found colonizing or infecting the oral cavities of hiv/aids patients. drug resistance during c. dubliniensis infection is common and constitutes a significant therapeutic challenge. the calcineurin inhibitor fk506 exhibits synergistic fungicidal activity with azoles or echinocandins in the fungal pathogens c. albicans, cryptococcus neoformans, and aspergillus fumigatus. in this study, ... | 2011 | 21531874 |