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transcatheter embolization of mycotic aneurysm of the subclavian artery with metallic coils.mycotic aneurysms of the subclavian artery are rare. this report describes an experience of 2 rare cases in which transcatheter embolization with metallic coils was performed for the management of these lesions alternative to surgery. two patients who had been treated with chemotherapy for malignant neoplasms were diagnosed as having mycotic aneurysms of the left subclavian artery. the causes of these lesions were presumed to be the invasion of the arterial wall by the pulmonary abscess in case ...200010952342
[bacteremia caused by stenotrophomonas maltophilia: epidemiology, clinical features, and prognosis factors].the epidemiologic, clinical and prognostic characteristics of stenotrophomonas maltophilia bacteremias for a six-year period (january 1993 to december 1998) were reviewed. twenty episodes of s. maltophilia bacteremia were include, which represented 0.62% of all bacteremias caused by gram-negative rods during that period. seventy percent of bacteremias were clinically significant. the most common predisposing factors were the previous use of antimicrobial agents and the presence of vascular cathe ...200010953584
endocarditis caused by stenotrophomonas maltophilia.stenotrophomonas maltophilia is frequently isolated from hospital environments. in recent years, it has been reported that this bacterium is causing hospital infections at increasing rates and is gaining importance because of its multiple resistance. although it has been related to several infections, endocarditis caused by s. maltophilia is rarely encountered. in this case study, endocarditis in a 40-y-old man with a history of aortic valve replacement is presented. blood cultures revealed s. m ...200010959657
[prevalence of microbiota in the digestive tract of wild females of lutzomyia longipalpis lutz & neiva, 1912) (diptera: psychodidae)].we dissected the digestive tract of 245 females in pools of 35 flies forming 7 groups. these flies were lutzomyia longipalpis originating from lapinha cave, lagoa santa, minas gerais. out of the 8 species of bacteria isolated there was a predominancy of gram negative bacterias (gnb) in the group of non-fermenters of sugar belonging to the following species: acinetobacter lwoffii, stenotrophomonas maltophilia, pseudomonas putida and flavimonas orizihabitans. the group of gnb fermenters were: ente ...200010967602
the use of silica gel prepared by sol-gel method and polyurethane foam as microbial carriers in the continuous degradation of phenol.a mixed microbial culture was immobilized by entrapment into silica gel (sg) and entrapment/ adsorption on polyurethane foam (pu) and ceramic foam. the phenol degradation performance of the sg biocatalyst was studied in a packed-bed reactor (pbr), packed-bed reactor with ceramic foam (pbrc) and fluidized-bed reactor (fbr). in continuous experiments the maximum degradation rate of phenol (q(s)max) decreased in the order: pbrc (598 mg l(-1) h(-1)) > pbr (pu, 471 mg l(-1)h(-1)) > pbr(sg, 394 mg l(- ...200010968628
stenotrophomonas maltophilia endocarditis of prosthetic aortic valve: report of a case and review of literature.stenotrophomonas maltophilia (previously known as xanthomonas maltophilia and pseudomonas maltophilia ) is an aerobic, nonfermenting, gram-negative bacillus, which has emerged as a serious nosocomial pathogen in patients with compromised immunity. it is a rare cause of endocarditis with only 20 cases previously reported in medical literature. the risk factors associated with s maltophilia endocarditis include intravenous drug abuse, dental treatment, previous cardiac surgery, and infected intrav ...200010986530
stenotrophomonas maltophilia infection in children. 200011001113
investigation of an outbreak of gram-negative bacteremia among hematology-oncology outpatients.to identify risk factors associated with an outbreak of gram-negative bacteremia (gnb).200011001264
bacteremia due to stenotrophomonas maltophilia in patients with hematologic malignancies.predisposing factors, clinical characteristics, and antimicrobial treatment of 37 hematology patients with stenotrophomonas maltophilia bacteremia who were seen at the department of hematology of the university la sapienza (rome) from 1987 to 1996 were evaluated. the results were compared with a control group of patients with pseudomonas aeruginosa bacteremia. profound neutropenia was more prolonged in the s. maltophilia group (p=.025), severe cellulitis occurred only in s. maltophilia-infected ...200011017819
design, synthesis, and sar of novel carbapenem antibiotics with high stability to xanthomonas maltophilia oxyiminocephalosporinase type ii.racemic cis-6-(phenylacetamido)carbapenem (21), 2-hydroxycarbonyl-cis-6-(phenylacetamido)carbapenem (22), 2-methoxycarbonyl-cis-6-(phenylacetamido)carbapenem (30), 2-methoxycarbomethyl-cis-6-(phenylacetamido)carbapenem (33), 2-hydroxyethyl-cis-6-(phenylacetamido)carbapenem (34), and 2-acetoxyethyl-cis-6-(phenylacetamido)carbapenem (35) were synthesized. formation of the carbapenem nuclei in 21, 22, and 30 involved dehydrophosphonation of the corresponding 2-diphenylphosphono-6-(phenylacetamido)c ...200011020277
cloning and characterization of smedef, a novel multidrug efflux pump from stenotrophomonas maltophilia.stenotrophomonas maltophilia is a nosocomial bacterial pathogen intrinsically resistant to several antibiotics. the mechanisms involved in this intrinsic multiresistance phenotype are poorly understood. a library of chromosomal dna from a spontaneous multidrug-resistant s. maltophilia d457r mutant (a. alonso and j. l. martinez, antimicrob. agents chemother. 41:1140-1142, 1997) was screened for complementation of erythromycin susceptibility on an antibiotic-hypersusceptible escherichia coli delta ...200011036026
carbapenemases: a problem in waiting?carbapenems are stable to most prevalent beta-lactamases, and chromosomal carbapenemases are restricted to stenotrophomonas maltophilia, to a few bacteroides fragilis, and to rare pathogens. nevertheless, an acquired metallo-beta-lactamase called imp-1 is beginning to emerge in pseudomonas aeruginosa and enterobacteriaceae isolates in japan, and has also been found in isolates from singapore. furthermore, imp-producing acinetobacter spp. have been identified in italy and hong kong. recently a se ...200011050448
enrichment and isolation of non-specific aromatic degraders from unique uncontaminated (plant and faecal material) sources and contaminated soils.microbial analysis of contaminated soil and uncontaminated plant and faecal material resulted in the enrichment of a number of microbial communities capable of utilizing a range of environmental pollutants. growth was observed on polycyclic aromatic hydrocarbons, polychlorinated biphenyls, heterocyclic aromatic compounds and organochlorine pesticides. however, none of the communities could grow on pentachlorophenol. pure cultures were isolated from microbial communities using phenanthrene and py ...200011054168
monitoring bacterial transport by stable isotope enrichment of cells.understanding the transport and behavior of bacteria in the environment has broad implications in diverse areas, ranging from agriculture to groundwater quality, risk assessment, and bioremediation. the ability to reliably track and enumerate specific bacterial populations in the context of native communities and environments is key to developing this understanding. we report a novel bacterial tracking approach, based on altering the stable carbon isotope value (delta(13)c) of bacterial cells, w ...200011055946
influence of an extended incubation period on values of minimum inhibitory concentrations of antibiotics in stenotrophomonas maltophilia clinical strains.in 106 stenotrophomonas maltophilia clinical strains the susceptibility to 19 kinds of antibiotics was tested by the broth dilution micromethod at 24 h and 48 h incubation. isolated strains demonstrated the lowest frequency of resistance to cotrimoxazole (7.5% of resistant strains at 24 h incubation and 18.9% at 48 h), ofloxacin (13.2% and 30.2%), ciprofloxacin (19.8% and 50.9%) and to cefoperazone/sulbactam (20.8% and 37.7%). the smallest growth of the number of resistant strains after extended ...200011061630
antibacterial spectrum of a novel des-fluoro(6) quinolone, bms-284756.the in vitro spectrum of a novel des-fluoro(6) quinolone, bms-284756, was compared with those of five fluoroquinolones (trovafloxacin, moxifloxacin, levofloxacin, ofloxacin, and ciprofloxacin). bms-284756 was among the most active and often was the most active quinolone against staphylococci (including methicillin-resistant strains), streptococci, pneumococci (including ciprofloxacin-nonsusceptible and penicillin-resistant strains), and enterococcus faecalis. bms-284756 inhibited approximately 6 ...200011083639
partial degradation of p-aminoazobenzene by a defined mixed culture of bacillus subtilis and stenotrophomonas maltophilia.a defined mixed culture of bacillus subtilis and stenotrophomonas maltophilia was used to accomplish the partial biodegradation of the azo-dye p-aminoazobenzene (paab). kinetic experiments were conducted, under aerobic conditions, to study the mineralization of p-aminoazobenzene by the above-defined mixed culture, under aerobic conditions. the combination of two previously developed models, (zissi et al., 1997), which describes paab biodegradation by bacillus subtilis into aniline and p-phenylen ...200111084593
waterborne nosocomial infections.waterborne pathogens cause infections in health-care facilities. despite guidelines addressing these pathogens, outbreaks and pseudo-outbreaks continue to occur. we reviewed recent reports of infections caused by pseudomonas aeruginosa, stenotrophomonas maltophilia, chryseobacterium species, nontuberculous mycobacteria, and legionella species. mycobacterium avium complex (mac) infection in hiv patients has been linked to hospital water distribution systems; molecular subtyping showed that mac is ...200011095897
nonrespiratory stenotrophomonas maltophilia infection at a children's hospital.to describe stenotrophomonas maltophilia infection in children, we reviewed the medical records of patients with isolates from nonrespiratory sites and identified 85 episodes, 51 (60%) of which represented true infection. forty-two episodes (82.4%) were hospital acquired. commonly associated with s. maltophilia infection were underlying illness (in 90.2% of cases), previous hospitalizations (in 78.7%), previous antibiotic exposure (in 78.4%), and the presence of a central venous catheter (in 76. ...200011095997
unusual respiratory bacterial flora in cystic fibrosis: microbiologic and clinical features.pulmonary infections continue to be a significant source of morbidity and mortality among patients with cystic fibrosis. although our understanding of the pathogenesis and clinical consequences of pulmonary infections with pseudomonas aeruginosa has increased greatly in recent years, very little is known about potentially emerging pathogens such as burkholderia cepacia complex, stenotrophomonas maltophilia, alcaligenes xylosoxidans, and methicillin-resistant staphylococcus aureus. in this review ...200011100967
identification and detection of stenotrophomonas maltophilia by rrna-directed pcr.stenotrophomonas maltophilia has recently emerged as an important nosocomial pathogen in immunocompromised patients, in transplant recipients, and in persons with cystic fibrosis (cf). while this organism is nonpathogenic in healthy individuals, it is increasingly associated with morbidity and mortality in susceptible populations. recent studies have indicated that for approximately 10% of cf patients with moderate lung disease, s. maltophilia can be cultured from respiratory tract secretions. i ...200011101555
a tem-2beta-lactamase encoded on an active tn1-like transposon in the genome of a clinical isolate of stenotrophomonas maltophilia.a constitutively expressed beta-lactamase gene from a clinical isolate of stenotrophomonas maltophilia, j675ia, has been cloned. its dna sequence is almost identical to that of bla(tem2) (one nucleotide change) and the expressed enzyme is a bush type 2a penicillinase with an amino acid sequence identical to that of tem-2. the bla(tem) gene was present within a novel tn1/tn3-type transposon in the genome of isolate j675ia and the transposon was able to mobilize bla(tem) on to the broad host-range ...200011102404
[sterilization of phacoemulsification and vitrectomy instruments. contamination and evaluation].contamination of automated surgical equipment is widely disregarded as a potential source of perioperative infection. we investigated the possibility of contamination of the aspiration fluid by the vacuum control manifold (vcm). the normal, unsterile internal vcm was compared with a modified external vcm that was regularly disinfected.200011105548
16s rdna sequence analysis of xylella fastidiosa strains.the 16s rdna encoding the small subunit ribosomal rna were amplified by pcr, cloned, and sequenced from 16 strains of xylella fastidiosa originating from nine different hosts. in pair-wise comparisons, x. fastidiosa strains showed a maximum variation of 1.0% or 14 nucleotide positions. when all 16 sequences were considered as a set, 54 variable positions were found. analysis of the sequence data indicated that the x. fastdiosa strains formed three rdna groups. group one includes pierce's disease ...200011108013
evaluation of current activities of fluoroquinolones against gram-negative bacilli using centralized in vitro testing and electronic surveillance.given the propensity for enterobacteriaceae and clinically significant nonfermentative gram-negative bacilli to acquire antimicrobial resistance, consistent surveillance of the activities of agents commonly prescribed to treat infections arising from these organisms is imperative. this study determined the activities of two fluoroquinolones, levofloxacin and ciprofloxacin, and seven comparative agents against recent clinical isolates of enterobacteriaceae, pseudomonas aeruginosa, acinetobacter b ...200111120976
comparison of antifungal activities and 16s ribosomal dna sequences of clinical and environmental isolates of stenotrophomonas maltophilia.in recent years, the gram-negative bacterium stenotrophomonas maltophilia has become increasingly important in biotechnology and as a nosocomial pathogen, giving rise to a need for new information about its taxonomy and epidemiology. to determine intraspecies diversity and whether strains can be distinguished based on the sources of their isolation, 50 s. maltophilia isolates from clinical and environmental sources, including strains of biotechnological interest, were investigated. the isolates ...200111136762
survival of stenotrophomonas maltophilia following exposure to concentrations of tobramycin used in aerosolized therapy for cystic fibrosis patients.the use of aerosolized tobramycin is an important component in the management of patients with cystic fibrosis, particularly those with chronic pseudomonas aeruginosa infection. clinicians have been concerned that long-term therapy with aerosolized tobramycin may increase the risk of colonization with multi-resistant bacteria, including stenotrophomonas maltophilia. the ability of five strains (three clinical, two environmental) of s. maltophilia to survive exposure to 16000 microg/ml tobramycin ...200111137651
influence of an infectious disease service on antibiotic prescription behavior and selection of multiresistant pathogens.a routine infectious disease service was established in january 1998 in order to optimize the antibiotic usage and prescription pattern of a neurologic intensive care unit (nicu).200011139159
[nosocomial infections in neonatal and pediatric intensive care. the appeal of ciprofloxacin].nosocomial infections, caused by multiresistant bacteria, are very common in neonatal intensive care units (niu) and they engage the vital prognosis. material and methods: from january 1994 to december 1995, 29 children suffered from nosocomial infections due to multiresistant bacteria. results: bacteria were isolated in blood cultures and/or in cerebrospinal fluid and included klebsiella (14 cases), enterobacter (eight cases), pseudomonas (three cases), acinetobacter (one case), stenotrophomona ...200011147060
effects of empiric antibiotic administration for suspected pneumonia on subsequent opportunistic pulmonary infections.optimal guidelines for empiric antibiotic (eab) therapy in cases of suspected post-traumatic ventilator-associated pneumonia (vap) are not well defined. eab administration is thought to increase the incidence of opportunistic organisms; however, culture-directed (as opposed to empiric) treatment may delay antibiotic administration with possible adverse consequences. our goal was to examine the impact of eab administration on the incidence of subsequent vap and opportunistic organisms in a series ...200011149581
comparison of serum bactericidal activity of ceftazidime, ciprofloxacin and meropenem against stenotrophomonas maltophilia. 200111152445
plasmid location and molecular heterogeneity of the l1 and l2 beta-lactamase genes of stenotrophomonas maltophilia.an approximately 200-kb plasmid has been purified from clinical isolates of stenotrophomonas maltophilia. this plasmid was found in all of the 10 isolates examined and contains both the l1 and the l2 beta-lactamase genes. the location of l1 and l2 on a plasmid makes it more likely that they could spread to other gram-negative bacteria, potentially causing clinical problems. sequence analysis of the 10 l1 genes revealed three novel genes, l1c, l1d, and l1e, with 8, 12, and 20% divergence from the ...200111158734
the in vitro activity of sulbactam combined with third generation cephalosporins against third generation cephalosporin-resistant bacteria.the in vitro activity of the beta-lactamase inhibitor sulbactam combined with cefuroxime, cefotaxime or ceftazidime in the ratio of 1:1 was studied against ceftazidime- or cefuroxime-resistant gram-negative rods and staphylococcus aureus. sulbactam enhanced the antibacterial activities of cefuroxime, cefotaxime and ceftazidime against gram-negative rods. the mic(90) of ceftazidime against escherichia coli, enterobacter cloacae, citrobacter freundii, acinetobacter spp. and pseudomonas aeruginosa ...200111165119
improved isolation of stenotrophomonas maltophilia from the sputa of patients with cystic fibrosis using a selective medium. 200011168159
resistance patterns among nosocomial pathogens: trends over the past few years.multiple surveillance studies have demonstrated that resistance among prevalent pathogens is increasing at an alarming rate, leading to greater patient morbidity and mortality from nosocomial infections. among gram-positive organisms, the most important resistant pathogens are methicillin- (oxacillin-)resistant staphylococcus aureus, beta-lactam-resistant and multidrug-resistant pneumococci, and vancomycin-resistant enterococci. important causes of gram-negative resistance include extended-spect ...200111171776
metallo-beta-lactamase producers in environmental microbiota: new molecular class b enzyme in janthinobacterium lividum.eleven environmental samples from different sources were screened for the presence of metallo-beta-lactamase-producing bacteria by using a selective enrichment medium containing a carbapenem antibiotic and subsequently testing each isolate for production of edta-inhibitable carbapenemase activity. a total of 15 metallo-beta-lactamase-producing isolates, including 10 stenotrophomonas maltophilia isolates, 3 chryseobacterium spp., one aeromonas hydrophila isolate, and one janthinobacterium lividum ...200111181369
[problems of antibiotic-resistance gram negative pathogens in the hospital environment].gram-negative pathogens in the hospital environment belong to such distinct families as enterobacteriaceae, pseudomonas, acinetobacter and stenotrophomonas. they differ in virulence but all present natural (or intrinsic) resistance and the capacity to develop rapidly acquired resistance, the main factor in their selection in the hospital environment. dissemination of resistance is due either to dissemination of a clonal strain or dissemination of the gene of resistance amongst bacteria belonging ...200011189438
the problem of a solvent exposable disulfide when preparing co(ii)-substituted metallo-beta-lactamase l1 from stenotrophomonas maltophilia.in an effort to prepare co(ii)-substituted metallo-beta-lactamase l1 from stenotrophomonas maltophilia for future spectroscopic and mechanistic studies, two methods for the preparation of co(ii)-l1 were tested. method a involved adding cocl2 directly to apo-l1 under anaerobic conditions. the resulting enzyme contained 1.9 moles of cobalt and exhibited very little activity, and uv-vis, 1h nmr, and epr studies indicated that most of the cobalt in this sample was co(iii). method b involved reducing ...200111191226
clinical implications of stenotrophomonas maltophilia resistant to trimethoprim-sulfamethoxazole: a study of 69 patients at 2 university hospitals.we conducted a retrospective case study at 2 tertiary care centers to determine the clinical implications of trimethoprim-sulfamethoxazole resistant stenotrophomonas maltophilia (tsrsm). of 69 reviewed cases (mean age, 57 y; male gender, 70%), 40 (58%) were classified as infections associated with tsrsm (respiratory tract, 14; soft tissue, 11; bloodstream, 8; other sites, 7). severe underlying comorbidities (86%) and previous antibiotic exposure (99%) were common. cefotetan (susceptibility, 55%) ...200011200376
the formation of migratory ripples in a mixed species bacterial biofilm growing in turbulent flow.mixed-species biofilms, consisting of klebsiella pneumoniae, pseudomonas aeruginosa, pseudomonas fluorescens and stenotrophomonas maltophilia, were grown in glass flow cells under either laminar or turbulent flow. the biofilms grown in laminar flow consisted of roughly circular-shaped microcolonies separated by water channels. in contrast, biofilm microcolonies grown in turbulent flow were elongated in the downstream direction, forming filamentous 'streamers'. moreover, biofilms growing in turbu ...199911207765
structures of the o4 and o18 antigens of stenotrophomonas maltophilia: a case of enantiomeric repeating units.the o-specific side-chain polymers of lipopolysaccharides from the reference strains for stenotrophomonas maltophilia serogroups 04 and o18 are both xylosylated rhamnans. in the 04 polymer, both sugar components are the d isomers, whereas the o18 polymer contains only the l isomers. by means of nmr spectroscopy, methylation analysis and smith degradation, the repeating unit of the 04 polymer was identified as a doubly-branched pentasaccharide of the structure shown below. the o18 polymer is base ...200111217974
structure of the o16 antigen of stenotrophomonas maltophilia.a polysaccharide containing d-ribose, n-acetyl-d-glucosamine, and n-acetyl-d-mannosamine was isolated from the phenol-soluble lipopolysaccharide extracted from defatted cell walls of the reference strain (560) for serogroup o16 of stenotrophomonas maltophilia. the results of methylation analysis, chemical degradations, and nmr spectroscopy showed that the polysaccharide is based on a branched trisaccharide repeating-unit of the structure shown below. although ribose was absent from about half of ...200111217982
prevalence of nonfermenters in clinical specimens.one hundred and thirty three non fermenting gram negative bacilli isolated out of 625 different clinical specimens were identified and characterised. samples were exudate from chronic suppractive otits media (341), diabetic foot (117) wound (116) and blood (51). of these isolates pseudomonas aeruginosa 105(78.94%) predominated followed by acinetobacter sp 8 [6.1%], pseudomonas putrifaciens 6(4.5%), flavobacterium sp 6(4.5%), xanthomonas maltophilia 5(3.75%), alkaligenes sp 3 (2.25). 31 (23.30%) ...200011227617
analysis of bacterial community structure in sulfurous-oil-containing soils and detection of species carrying dibenzothiophene desulfurization (dsz) genes.the selective effects of sulfur-containing hydrocarbons, with respect to changes in bacterial community structure and selection of desulfurizing organisms and genes, were studied in soil. samples taken from a polluted field soil (a) along a concentration gradient of sulfurous oil and from soil microcosms treated with dibenzothiophene (dbt)-containing petroleum (fsl soil) were analyzed. analyses included plate counts of total bacteria and of dbt utilizers, molecular community profiling via soil d ...200111229891
[digestive tract microbiota in female lutzomyia longipalpis (lutz & neiva, 1912) (diptera: psychodidae) from colonies feeding on blood meal and sucrose plus blood meal].there are very few reports on the microbiota of the digestive tract of sand flies, an important omission considering that blood is not the only meal ingested. male and female sand flies obtain sugar meals from several sources, thereby increasing their chance of infection with microorganisms. chances of contamination are higher when insects are bred in the laboratory, and this may affect the development of leishmania spp. from the digestive tract of 300 sand fly females separated in two groups we ...200111241946
comparative activities of isepamicin, amikacin, cefepime, and ciprofloxacin alone or in combination with other antibiotics against stenotrophomonas maltophilia. 200111245333
characterization of oxytetracycline-resistant heterotrophic bacteria originating from hospital and freshwater fishfarm environments in england and ireland.this ecotaxonomic study compared the antibiotic tolerance among culturable oxytetracyline-resistant (ot(r)) heterotrophic strains isolated from two aquatic environments representing human activities in health care and aquaculture, namely hospital effluents and freshwater fishfarms. using a standardized methodology, samples taken in england and ireland were analyzed to determine the antibiotic tolerance profiles of two groups of culturable ot(r) bacterial isolates at the intergeneric and intragen ...200011249032
susceptibility of burkholderia cepacia and other pathogens of importance in cystic fibrosis to u.v. light.to investigate the potential usefulness of u.v. germicidal irradiation (uvgi) in preventing the spread of burkholderia cepacia, an important pathogen in cystic fibrosis (cf), the in-vitro susceptibility of b. cepacia to uvgi was determined. five strains were exposed to uvgi from a 7.2-w source. burkholderia cepacia was less susceptible to uvgi than other important cf-related pathogens, namely staphylococcus aureus and pseudomonas aeruginosa, but was more susceptible than stenotrophomonas maltoph ...200111264740
persistence and variability of stenotrophomonas maltophilia in cystic fibrosis patients, madrid, 1991-1998.during 1991 to 1998 at least one stenotrophomonas maltophilia pulmonary infection was observed in 25 (24%) of 104 cystic fibrosis patients at the same unit of our hospital in spain. ribotyping and pulse-field gel electrophoresis (pfge) characterization of 76 s. maltophilia isolates from these patients indicated an overall clonal incidence of 47.1%, reflecting new strains in 44% of patients with repeated positive cultures for s. maltophilia. six patients with repeated episodes were persistently c ...200111266301
hospital infestation by the cluster fly, pollenia rudis sensu stricto fabricius 1794 (diptera: calliphoridae), and its possible role in transmission of bacterial pathogens in germany.the potential of the cluster fly, pollenia rudis sensu stricto, to transmit bacterial pathogens was investigated during a mass infestation that took place in a german hospital. cluster flies were individually examined for mesophilic bacteria carried on the exoskeleton. bacterial growth could only be detected by using the enrichment culture technique to increase sensitivity, but not by direct intoculation of fly samples to agar plates. all 50 cluster fly samples that were tested carried opportuni ...200111279815
multicentre study of the in vitro activity of cefepime, a broad-spectrum cephalosporin, compared to other broad-spectrum agents.the in vitro activity of cefepime was compared to that of a range of other broad-spectrum agents, using a gradient diffusion mic method, against 995 recent clinical isolates of enterobacteriaceae, pseudomonas aeruginosa, other nonfermentative gram-negative bacilli, staphylococci (except oxacillin-resistant staphylococcus aureus), streptococci, enterococci, and aerobic gram-positive bacilli. cefepime had excellent activity against enterobacteriaceae, including eight presumptive extended-spectrum ...200111280610
in vitro activity of moxifloxacin against stenotrophomonas maltophilia blood isolates from patients with hematologic malignancies. 200111284945
clustering of infections caused by different pfge types of stenotrophomonas maltophilia occurring simultaneously in a university hospital. 200111289781
increasing threat of gram-negative bacteria.the widespread use of broad-spectrum antibiotics has led to emergence of antibiotic-resistant strains of many gram-negative organisms. this problem is particularly serious in critically ill patients, especially those with ventilator-associated pneumonia. extensive antibiotic resistance has developed in gram-negative bacteria, due both to innate resistance in some species and the fact that they are highly adept at acquiring antibiotic-resistant determinants from each other. antibiotic resistance ...200111292879
antimicrobial susceptibilities of unique stenotrophomonas maltophilia clinical strains.susceptibility to 41 antimicrobials was studied with 99 stenotrophomonas maltophilia strains, and different pulsed-field gel electrophoresis profiles were identified among 130 prospectively collected isolates. moxalactam, doxycycline, minocycline, and clinafloxacin displayed the highest activity (> or = 98% susceptibility). ticarcillin resistance (75%) was reverted by clavulanate in 25% of strains. trimethoprim-sulfamethoxazole resistance was 26.2% (> or = 4 [trimethoprim]/76 [sulfamethoxazole] ...200111302834
emerging importance of multidrug-resistant acinetobacter species and stenotrophomonas maltophilia as pathogens in seriously ill patients: geographic patterns, epidemiological features, and trends in the sentry antimicrobial surveillance program (1997-1999).as part of the sentry antimicrobial surveillance program, a total of 1078 acinetobacter species and 842 stenotrophomonas maltophilia isolates were collected between january 1997 and december 1999 from 5 geographic regions (canada, the united states, latin america, europe, and the asia-pacific). the frequency of infections (by geographic region and body site), including those due to imipenem-resistant acinetobacter species and trimethoprim-sulfamethoxazole (tmp-smz)-resistant s. maltophilia, was ...200111320451
integration of molecular characterization of microorganisms in a global antimicrobial resistance surveillance program.the sentry antimicrobial surveillance program has incorporated molecular strain typing and resistance genotyping as a means of providing additional information that may be useful for understanding pathogenic microorganisms worldwide. resistance phenotypes of interest include multidrug-resistant pathogens, extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae, methicillin-resistant staphylococcus aureus (mrsa), vancomycin-resistant enterococci, and fluoroquinolone-resistant (fqr) s ...200111320455
complete mineralization of dodecyldimethylamine using a two-membered bacterial culture.complete degradation of dodecyldimethylamine was achieved using a two-membered bacterial culture isolated from activated sludge. one member, identified as burkholderia cepacia, was capable of degrading the alkyl chain of the molecule. the other member, identified as stenotrophomonas maltophilia, was able to degrade dimethylamine, the product of the former. batch culture experiments revealed that the two-membered culture consisting of b. cepacia and s. maltophilia was based on a commensalistic re ...200111321543
multidrug efflux pumps and antimicrobial resistance in pseudomonas aeruginosa and related organisms.pseudomonas aeruginosa is an opportunistic human pathogen characterized by an innate resistance to multiple antimicrobial agents. a major contribution to this intrinsic multidrug resistance is provided by a number of broadly-specific multidrug efflux systems, including mexab-oprm and mexxy-oprm. in addition, these and two additional tripartite efflux systems, mexcd-oprj and mexef-oprn, promote acquired multidrug resistance as a result of mutational hyperexpression of the efflux genes. in additio ...200111321581
antibiotic use and development of resistance in blood culture isolates: 8 years of experience from a cancer referral center.the consumption of antimicrobial agents in a slovakian national cancer institute from 1989-1996 was compared with resistance rates in clinically significant blood culture isolates. we observed an increase in resistance of viridans streptococci to penicillin and of enterococci to ampicillin. resistance to vancomycin and teicoplanin was stable over the entire period despite a 20-fold increase in vancomycin consumption. nor did we observe increased resistance to ofloxacin despite a 10-fold increase ...200111330359
susceptibility to antimicrobial agents of stenotrophomonas maltophilia isolated from patients with cancer and bacteremia. 200111340542
expression of multidrug efflux pump smedef by clinical isolates of stenotrophomonas maltophilia.the presence of the multidrug efflux pump smedef was assessed in a collection of clinical isolates of stenotrophomonas maltophilia. all isolates encoded this pump, as demonstrated by pcr. forty-seven percent of the strains overproduced a protein of the same size that was immunoreactive against an anti-smef antibody, and 33% overexpressed the gene semd when they were tested by reverse transcription-pcr. a correlation between smedef overexpression and antibiotic resistance was observed.200111353642
[infections caused by stenotrophomonas maltophilia (sma) in intensive care patients--a prospective case-control study].the importance of stenotrophomonas maltophilia (sma) as an etiologic frequently polyresistant pathogen in severe nosocomial infections has increased.200111381633
comparison of the antimicrobial tolerance of oxytetracycline-resistant heterotrophic bacteria isolated from hospital sewage and freshwater fishfarm water in belgium.the aim of this study was to investigate the relationship between antimicrobial tolerance and taxonomic diversity among the culturable oxytetracycline-resistant (ot(r)) heterotrophic bacterial population in two belgian aquatic sites receiving wastewater either from human medicine or from aquaculture. the study of ot(r) heterotrophs and mesophilic aeromonas spp. allowed comparison of tolerance data at the intergenus as well as at the intragenus level. in total, 354 independently obtained ot(r) is ...200111403391
antimicrobial activities of gatifloxacin against nosocomial isolates of stenotrophomonas maltophilia measured by mic and time-kill studies.we determined in vitro activities of gatifloxacin and seven other drugs against 100 isolates of stenotrophomonas maltophilia using the agar gradient diffusion (etest) method. percentages of susceptible isolates were as follows: trimethoprim-sulfamethoxazole, 90%; gatifloxacin, 71%; levofloxacin, 57%; ticarcillin-clavulanic acid, 54%; ceftazidime, 49%; ciprofloxacin, 29%; cefepime, 21%; and piperacillin-tazobactam, 20%. time-kill studies of three isolates indicated that gatifloxacin was bacterici ...200111408235
antibiotic resistance surveillance of stenotrophomonas maltophilia, 1993-1999. 200111418532
recommendations for susceptibility tests on fastidious organisms and those requiring special handling.fastidious organisms present problems in antimicrobial susceptibility testing related to particular cultural requirements or slow growth. methods for commonly isolated fastidious organisms, including haemolytic streptococci, neisseria spp. and haemophilus spp., are included in the description of the standardized disc diffusion method. however, some further information is given here for haemophilus influenzae and neisseria spp. other less commonly isolated fastidious organisms are detailed and mo ...200111420340
binary culture biofilm formation by stenotrophomonas maltophilia and fusarium oxysporum.binary culture biofilm formation by stenotrophomonas maltophilia and fusarium oxysporum was investigated using the recirculating modified robbins device batch culture system. sequential attachment studies were carried out in the robbins device on pvc and glass surfaces, with each species as either the first or the second colonizer. different surfaces had no significant effect on total numbers of s. maltophilia and f. oxysporum in the binary population biofilm. the attachment of the second coloni ...200111420659
[trends for bacteremia and risk factors for death in a tertiary hospital in mexico city. 1981-1992].to describe the trends and risk factors of death for bacteremia in adult from a tertiary-care center from 1981 to 1992.200111432088
surveillance of antibiotic resistance in european icus.antibiotic resistance among bacteria causing hospital-acquired infections poses a threat, particularly to patients in intensive care units (icus). in order to control the spread of resistant bacteria, local, regional and national resistance surveillance data must be used to develop efficient intervention strategies. in an attempt to identify national differences and the dynamics of antibiotic resistance in european icus, data have been merged from several networks of resistance surveillance perf ...200111439002
resistance to antibiotics and biocides among non-fermenting gram-negative bacteria.to investigate the antibiotic and biocide susceptibilities of clinical isolates of rarely encountered gram-negative, non-fermenting bacteria.200111442564
long-term preservation of strains of burkholderia cepacia, pseudomonas spp. and stenotrophomonas maltophilia isolated from patients with cystic fibrosis.long-term preservation methods are important in the maintenance of bacteria for downstream research applications. most clinical laboratories have only limited resources for archiving isolates and therefore require cost-effective and simple methods. an effective and cheap storage method using debrinated blood and maintenance at -80 degrees c is described.200111442821
novel mechanism of hydrolysis of therapeutic beta-lactams by stenotrophomonas maltophilia l1 metallo-beta-lactamase.stopped-flow tryptophan fluorescence under single turnover and pseudo-first-order conditions has been used to investigate the kinetic mechanism of beta-lactam hydrolysis by the stenotrophomonas maltophilia l1 metallo-beta-lactamase. for the cephalosporin substrates nitrocefin and cefaclor and the carbapenem meropenem, a substantial quench of fluorescence is observed on association of substrate with enzyme. we have assigned this to a rearrangement event subsequent to formation of an initial colli ...200111443136
production of n-acyl homoserine lactones by gram-negative bacteria isolated from contact lens wearers.the purpose of this study was to investigate the production of n-acyl-homoserine lactone (ahl) signal molecules in ocular gram-negative bacteria. a total of 91 ocular strains isolated from contact lens adverse response patients and asymptomatic subjects were used in the study. these included acinetobacter, aeromonas hydrophila, escherichia coli, haemophilus influenzae, klebsiella oxytoca, pseudomonas aeruginosa, serratia liquefaciens, serratia marcescens, and stenotrophomonas maltophilia. the bi ...200111446456
comparative in vitro activity of gemifloxacin, ciprofloxacin, levofloxacin and ofloxacin in a north american surveillance study.the in vitro activity of gemifloxacin, a new fluoroquinolone, was compared to three marketed fluoroquinolones; ciprofloxacin, levofloxacin and ofloxacin against over 4,000 recent clinical isolates covering 29 species isolated in the united states and canada between 1997-1999. based on mic(90)s, gemifloxacin was the most potent fluoroquinolone tested against a majority of gram-positive isolates: streptococcus pneumoniae, penicillin resistant s. pneumoniae, macrolide resistant s. pneumoniae, cipro ...200111448564
intralenticular abscess caused by stenotrophomonas maltophilia. 200111450743
stenotrophomonas maltophilia endophthalmitis after penetrating injury by a wooden splinter. 200111450746
antibiotic resistance among gram-negative non-fermentative bacteria at a teaching hospital in saudi arabia.the incidence and antimicrobial resistance of gram-negative non-fermentative bacteria isolated over 1 year at king abdulaziz university hospital, jeddah, saudi arabia were investigated. a total of 499 of these microorganisms were collected and account for 16% of all gram-negative bacteria isolated. the most common species were pseudomonas aeruginosa 291 (56%), acinetobacter baumannii 170 (34%), and stenotrophomonas maltophilia 35 (7%). 168 (34%) of these microorganisms were isolated from intensi ...200111450883
isolation and characterisation of new gram-negative and gram-positive atrazine degrading bacteria from different french soils.the capacity of 12 soils to degrade atrazine was studied in laboratory incubations using radiolabelled atrazine. eight soils showed enhanced degradation of this compound. twenty-five bacterial strains able to degrade atrazine were isolated by an enrichment method from 10 of these soils. these soils were chosen for their wide range of physico-chemical characteristics. their history of treatment with atrazine was also variable. the genetic diversity of atrazine degraders was determined by amplifie ...200111451526
efficacy of cefepime versus ceftazidime in the treatment of adult pneumonia.effective empiric treatment of pneumonia requires antibiotic coverage against gram-negative and gram-positive pathogens, including drug-resistant isolates. this study evaluated the efficacy of cefepime treatment in 20 patients with community-acquired pneumonia (cap) and 21 patients with hospital-acquired pneumonia (hap), and ceftazidime treatment in 20 patients with hap. the mean age of patients was over 70 years. more than half of the patients had multiple lobe involvement. there was no signifi ...200111456359
growth of chitinolytic dune soil beta-subclass proteobacteria in response to invading fungal hyphae.it has frequently been reported that chitinolytic soil bacteria, in particular biocontrol strains, can lyse living fungal hyphae, thereby releasing potential growth substrate. however, the conditions used in such assays (high bacterial density, rich media, fragmented hyphae) make it difficult to determine whether mycolytic activity is actually of importance for the growth and survival of chitinolytic bacteria in soils. an unidentified group of beta-subclass proteobacteria (cbetaps) was most domi ...200111472904
polymicrobial outbreak of intermittent peritoneal dialysis peritonitis during external wall renovation at a dialysis center.to investigate an outbreak of peritonitis in intermittent peritoneal dialysis (ipd) patients.200111475346
septicemia associated with stenotrophomonas maltophilia in a west african dwarf crocodile (osteolaemus tetraspis subsp. tetraspis).a 17-year-old male captive west african dwarf crocodile (osteolaemus tetraspis subsp. tetraspis) died 1 month after fighting with a penmate. abrasions were present on the head and mandible. necropsy revealed a vegetative valvular lesion of the left atrioventricular valve, miliary foci of necrosis in the endocardium and myocardium, multiple duodenal and rectal ulcers, and serous atrophy of body fat. stenotrophomonas maltophilia was isolated in pure culture from lung, liver, and kidney. gram-negat ...200111482606
occurrence of variants with temperature-dependent susceptibility (tds) to antibiotics among stenotrophomonas maltophilia clinical strains.susceptibility to 20 antibiotics was tested in 104 stenotrophomonas maltophilia strains at 37 and 30 degrees c by means of a dilution micromethod to verify the phenomenon of temperature-dependent susceptibility (tds). trimethoprim-sulfamethoxazole, pefloxacin and ofloxacin were the most active preparations at 37 degrees c (93, 90, and 86% of susceptible strains, respectively), whilst trimethoprim-sulfamethoxazole, cefoperazone-sulbactam and pefloxacin performed best at 30 degrees c (94, 94, and ...200111501404
identity and potential functions of heterotrophic bacterial isolates from a continuous-upflow fixed-bed reactor for denitrification of drinking water with bacterial polyester as source of carbon and electron donor.a collection of 186 heterotrophic bacteria, isolated directly from a continuous-upflow fixed-bed reactor for the denitrification of drinking water, in which poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (phbv) granules acted as biofilm carrier, carbon source and electron donor, was studied with regard to taxonomic affiliation and degradation and denitrification characteristics. two granule samples were taken from a fully operating reactor for enumeration and isolation of heterotrophic bacteria. o ...200111518336
evaluation of the vitek 2 system for the identification and susceptibility testing of three species of nonfermenting gram-negative rods frequently isolated from clinical samples.vitek 2 is a new automatic system for the identification and susceptibility testing of the most clinically important bacteria. in the present study 198 clinical isolates, including pseudomonas aeruginosa (n = 146), acinetobacter baumannii (n = 25), and stenotrophomonas maltophilia (n = 27) were evaluated. reference susceptibility testing of cefepime, cefotaxime, ceftazidime, ciprofloxacin, gentamicin, imipenem, meropenem, piperacillin, tobramycin, levofloxacin (only for p. aeruginosa), co-trimox ...200111526158
[antibiotic therapy of cystic fibrosis in children].it is postulated that p. aeruginosa in monoculture or in association with staphylococcus aureus keeps its leading position in chronic bacterial inflammatory broncho-pulmonary processes in children with cystic fibrosis. antibiotic resistant strains of burkholderia cepacia, stenotrophomonas maltophila, alcaligenes xylosoxidans were revealed (7.1% of the strains). p. aeruginosa strains were susceptible to aminoglycosides, ciprofloxacin, and polymixin b. susceptibility of smooth and mucoid forms of ...200111544748
infections caused by stenotrophomonas maltophilia--a prospective study.stenotrophomonas maltophilia is an opportunistic microorganism, often highly resistant to routinely tested antibiotics. this microorganism is isolated in specimens from patients with nosocomial infections with increasing frequency.200111545481
mineralization and co-metabolic degradation of phenoxyalkanoic acid herbicides by a pure bacterial culture isolated from an aquifer.a mecoprop [(+/-)-2-(4-chloro-2-methylphenoxy)propionic acid; mcpp]-degrading bacterium identified as stenotrophomonas maltophilia pm was isolated from a danish aquifer. besides mecoprop, the bacterium was also able to degrade mcpa [(4-chloro-2-methylphenoxy)acetic acid)], mcpb [(4-chloro-2-methylphenoxy)butyric acid], 4-cpa [(4-chlorophenoxy)acetic acid], 2, 4-d [(2, 4-dichlorophenoxy)acetic acid], 2, 4-dp [(+/-)-2-(2, 4-dichlorophenoxy)propionic acid] and 2, 4-db [(2, 4-dichlorophenoxy)butyric ...200111549024
microbiological and immunologic considerations with aerosolized drug delivery.the development of drug resistance is a major theoretical concern with the long-term delivery of aerosolized antibiotics via inhalation. a randomized, placebo-controlled, double-blind study, which compared inhaled tobramycin plus standard cystic fibrosis (cf) care to placebo plus standard cf care, examined the following microbiological parameters: percentage of patients with at least one pseudomonas aeruginosa (pa) strain with a minimal inhibitory concentration (mic) > 16 microg/ml (ie, the brea ...200111555566
cathelicidin peptides inhibit multiply antibiotic-resistant pathogens from patients with cystic fibrosis.endogenous peptide antibiotics are under investigation as inhaled therapeutic agents for cystic fibrosis (cf) lung disease. the bactericidal activities of five cathelicidin peptides (ll37 [human], cap18 [rabbit], mcramp [mouse], rcramp [rat], and smap29 [sheep]), three novel alpha-helical peptides derived from smap29 and termed ovispirins (ov-1, ov-2, and ov-3), and two derivatives of cap18 were tested by broth microdilution assays. their mics were determined for multiply antibiotic-resistant ps ...200111557478
use of random amplified polymorphic dna pcr to examine epidemiology of stenotrophomonas maltophilia and achromobacter (alcaligenes) xylosoxidans from patients with cystic fibrosis.stenotrophomonas maltophilia and achromobacter (alcaligenes) xylosoxidans have been increasingly recognized as a cause of respiratory tract colonization in cystic fibrosis (cf). although both organisms have been associated with progressive deterioration of pulmonary function, demonstration of causality is lacking. to examine the molecular epidemiology of s. maltophilia and a. xylosoxidans in cf, isolates from patients monitored for up to 2 years were fingerprinted using a pcr-based randomly ampl ...200111574579
fluoroquinolone susceptibilities of efflux-mediated multidrug-resistant pseudomonas aeruginosa, stenotrophomonas maltophilia and burkholderia cepacia.the antibacterial activities of seven fluoroquinolones (ciprofloxacin, bayy3118, clinafloxacin, gemifloxacin, moxifloxacin, sparfloxacin and trovafloxacin) against isogenic efflux-mediated multidrug-resistant strains of pseudomonas aeruginosa, stenotrophomonas maltophilia and burkholderia cepacia, were compared. the results indicate that these fluoroquinolones are all substrates for the multidrug efflux systems of these organisms. clinafloxacin was found generally to be the most active agent aga ...200111581236
stenotrophomonas maltophilia interaction with human epithelial respiratory cells in vitro.bacteria of stenotrophomonas maltophilia have been isolated with increasing frequency from the airways of cystic fibrosis (cf) patients, usually following p. aeruginosa infections, but their adherence to human epithelial respiratory cells has never been investigated. in this study, various s. maltophilia strains were seen to adhere to epithelial respiratory cells in vitro, mainly along intercellular junctions. bacteria could also enter into host cells, as determined by the gentamicin exclusion a ...200111592630
stenotrophomonas maltophilia meningitis - a rare cause of headache in the ed. 200111593485
endocarditis caused by stenotrophomonas maltophilia. 199911593523
[utility of prolonged incubation and terminal subcultures of blood cultures from immunocompromised patients].the value of blind terminal subcultures (7 and 30 days) and prolonged incubation (30 days) of blood cultures from immunosuppressed patients was analyzed in the fundación favaloro, the fundación para la lucha contra las enfermedades neurológicas de la infancia and the hospital de niños ricardo gutiérrez. a total of 2707 blood cultures and 369 patients were included (transplantation of solid organs 154, oncohematologic disorders 106 and solid tumors 109). bact-alert bottles were incubated at 35 de ...200111594009
synergistic activities of gatifloxacin in combination with other antimicrobial agents against pseudomonas aeruginosa and related species.drug combinations have been used to treat serious infections caused by pseudomonas, burkholderia, stenotrophomonas, and acinetobacter. in this study, the combined drug effects of gatifloxacin (gat) and nonquinolones were determined by time-kill analysis at clinically achievable drug concentrations. synergy (>or=2 log(10)-enhanced killing at 24 h) was observed with gat plus amikacin or a beta-lactam against 50 to 75% of strains, including strains nonsusceptible to one or both drugs.200111600384
in vitro activity of moxifloxacin against common clinical bacterial isolates in taiwan.the in vitro antimicrobial activities of moxifloxacin were compared with 7 other antimicrobial agents. a total of 707 isolates of 11 common pathogenic bacteria were collected from the national taiwan university hospital; antimicrobial activities against these isolates were evaluated by minimum inhibitory concentration using an agar-dilution method. most common pathogenic bacteria were susceptible to moxifloxacin, including methicillin-susceptible and -resistant staphylococcus aureus, methicillin ...200111605808
[new aspects of antibiotic resistance and possibilities of its prevention].new phenomena of the antibiotic resistance in bacteria have recently appeared. the may hold present explosive development of resistance and prevent its transferability from multiple drug resistant bacteria to still sensitive ones. they may prevent the production of so-called extended-spectrum beta-lactamases (esbls) among enterobacteriaceae producing resistance virtually to all penicillins and cephalosporins with exception of those antibiotics potentiated by clavulanic acid or sulbactam, the res ...200111678026
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