Publications

antimicrobial activity of water and acetone extracts of some eucomis taxa.	to evaluate the antibacterial and antifungal properties of acetone and water extracts of selected eucomis taxa.	2017	29080618
intrinsic short-tailed azole resistance in mucormycetes is due to an evolutionary conserved aminoacid substitution of the lanosterol 14α-demethylase.	mucormycoses are emerging and potentially lethal infections. an increase of breakthrough infections has been found in cohorts receiving short-tailed azoles prophylaxis (e.g. voriconazole (vcz)). although vcz is ineffective in vitro and in vivo, long-tailed triazoles such as posaconazole remain active against mucormycetes. our goal was to validate the molecular mechanism of resistance to short-tailed triazoles in mucorales. the paralogous cytochrome p450 genes (cyp51 f1 and cyp51 f5) of rhizopus ...	2017	29162893
development of a plasmid free crispr-cas9 system for the genetic modification of mucor circinelloides.	mucor circinelloides and other members of mucorales are filamentous fungi, widely used as model organisms in basic and applied studies. although genetic manipulation methods have been described for some mucoral fungi, construction of stable integrative transformants by homologous recombination has remained a great challenge in these organisms. in the present study, a plasmid free crispr-cas9 system was firstly developed for the genetic modification of a mucoral fungus. the described method offer ...	2017	29196656
identification and characterization of diacylglycerol acyltransferase from oleaginous fungus mucor circinelloides.	acyl-coa:diacylglycerol acyltransferase (dgat) is a pivotal regulator of triacylglycerol (tag) synthesis. the oleaginous fungus mucor circinelloides has four putative dgats: mcdgat1a, mcdgat1b, mcdgat2a, and mcdgat2b, classified into the dgat1 and dgat2 subfamilies, respectively. to identify and characterize dgats in m. circinelloides, these four genes were expressed in saccharomyces cerevisiae h1246 (tag-deficient quadruple mutant), individually. tag biosynthesis was restored only by the expres ...	2018	29260551
control of morphology and virulence by adp-ribosylation factors (arf) in mucor circinelloides.	mucor circinelloides is a dimorphic fungus used to study cell differentiation that has emerged as a model to characterize mucormycosis. in this work, we identified four adp-ribosylation factor (arf)-encoding genes (arf1-arf4) and study their role in the morphogenesis and virulence. arfs are key regulators of the vesicular trafficking process and are associated with both growth and virulence in fungi. arf1 and arf2 share 96% identity and arf3 and arf4 share 89% identity, which suggests that the g ...	2017	29264641
mucor circinelloides induces platelet aggregation through integrin αiibβ3 and fcγriia.	thrombosis is a hallmark of the fatal fungal infection mucormycosis. yet, the platelet activation pathway in response to mucormycetes is unknown. in this study we determined the platelet aggregation potential of mucor circinelloides (m. circinelloides) nrrl3631, characterized the signaling pathway facilitating aggregation in response to fungal spores, and identified the influence of the spore developmental stage upon platelet aggregation potential. using impedance and light-transmission aggregom ...	2018	29297721