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[peptostreptococcus endocarditis: presentation of two cases and literature review].we describe two new cases of peptostreptococcus endocarditis, one case of peptostreptococcus micros prosthetic valve endocarditis and the other of peptostreptococcus assaccharolyticus tricuspid native valve endocarditis in an intravenous drug user (idu) patient and review nine cases previously reported.200818769744
photodynamic therapy as an adjunct to non-surgical periodontal treatment: a randomized, controlled clinical trial.recent preclinical and clinical data have suggested a potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of this study was to evaluate the clinical and microbiologic effects of the adjunctive use of pdt to non-surgical periodontal treatment.200818771363
targeted antimicrobial activity of a specific igg-smap28 conjugate against porphyromonas gingivalis in a mixed culture.antimicrobial peptides coupled to a ligand, receptor or antibody for a specific pathogenic bacteria could be used to develop narrow-spectrum pharmaceuticals with 'targeted' antimicrobial activity void of adverse reactions often associated with the use of broad-spectrum antibiotics. to assess the feasibility of this approach, in this study sheep myeloid antimicrobial peptide (smap) 28 was linked to affinity- and protein g-purified rabbit immunoglobulin g (igg) antibodies specific to the outer sur ...200918778918
[bacteremia after periodontal procedures].bacteremia frequently occurs after oral surgery and odontology procedures. periodontitis may affect the incidence and bacterial spectrum of bacteremia. periodontal disease may be a significant risk factor for the development of certain systemic diseases. this study has aimed to evaluate the frequency of aerobic and anaerobic bacteria in the bloodstream following scaling and root planing.200818792815
correlates of periodontal decline and biologic markers in older adults.there is limited information on infectious and host responses distinguishing older people with or without active periodontitis. this study measured bacterial and serum cytokine and high-sensitivity c-reactive protein (hscrp) levels in older persons.200818834247
tracer experiments on the mechanism of glycine fermentation by diplococcus glycinophilus. 194818910737
the effect of a supragingival plaque-control regimen on the subgingival microbiota in smokers and never-smokers: evaluation by real-time polymerase chain reaction.the aim of the present study was to evaluate the effect of strict supragingival plaque control on the subgingival microbiota in smokers and never-smokers. research into the impact of supragingival plaque control on the number of subgingival bacteria has resulted in contradictory findings. real-time polymerase chain reaction (pcr) has been suggested as a valid alternative to current microbiologic methods based on bacteria cultures.200819053920
effectiveness of ozone against endodontopathogenic microorganisms in a root canal biofilm model.to assess the antimicrobial efficacy of aqueous (1.25-20 microg ml(-1)) and gaseous ozone (1-53 g m(-3)) as an alternative antiseptic against endodontic pathogens in suspension and a biofilm model.200919125975
short-term clinical and microbiological evaluations of peri-implant diseases before and after mechanical anti-infective therapies.the aim of this study was to evaluate the clinical and microbiological effects of mechanical anti-infective therapies for mucositis and peri-implantitis.200919126114
the microbiota of acute apical abscesses.as the breadth of bacterial diversity in the oral cavity has been deciphered by molecular studies, several newly identified species/phylotypes have emerged as potential pathogens. we hypothesized that many of these species/phylotypes could also be involved with the etiology of endodontic abscesses. abscess aspirates from 42 persons were analyzed for the presence of 81 species/phylotypes by means of a reverse-capture checkerboard hybridization assay. associations between the most frequently detec ...200919131319
new bacterial compositions in root-filled teeth with periradicular lesions.the aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. specimens were sampled from patients undergoing root canal retreatment. the bacteria were characterized by morphologic and biochemical analysis and by 16s rrna gene sequencing. microorganisms were detected in 10 of 18 teeth. the majority of positive samples revealed a mixed culture of 2-8 species. in 2 teeth enterococcus faecalis was the only detected species. for the first ...200919166766
lysozyme and microbiota in relation to gingivitis and periodontitis.the aim of this study was to investigate associations between the immune component of the oral cavity, lysozyme, in gingival crevicular fluid and unstimulated saliva, and colonization dynamics of periodontopathogenic bacteria from supra- and subgingival plaque samples in patients with gingivitis or periodontitis.200919179970
does pregnancy have an impact on the subgingival microbiota?we investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care.200919228092
collagenase production and hemolytic activity related to 16s rrna variability among parvimonas micra oral isolates.parvimonas micra are gram positive anaerobic cocci isolated from the oral cavity and frequently related to polymicrobial infections in humans. despite reports about phenotypic differences, the genotypic variation of p. micra and its role in virulence are still not elucidated. the aim of this study was to determine the genotypic diversity of p. micra isolates obtained from the subgingival biofilm of subjects with different periodontal conditions and to correlate these findings with phenotypic tra ...201019351562
the microbiota on different oral surfaces in healthy children.knowledge of the early oral colonization patterns could provide a better understanding of oral biofilm development and disease initiation that in turn could be the basis for early preventive programmes.200919416446
bacteria in the apical root canal of teeth with primary apical periodontitis.bacteria settled in the apical root canal are in a privileged position to inflict damage to the periradicular tissues. therefore, the species identified in this region can be of special relevance for the pathogenesis of apical periodontitis. this study investigated the occurrence and levels of several bacterial taxa in the apical root canal of teeth with apical periodontitis.200919426923
methods for optimizing dna extraction before quantifying oral bacterial numbers by real-time pcr.methods for the optimal extraction of genomic dna for real-time pcr enumeration of oral bacteria using the muramidase, mutanolysin, were developed using a simple in vitro oral flora model comprised of the facultative anaerobic gram-positive bacteria, lactobacillus acidophilus and streptococcus mutans, the gram-positive anaerobe, parvimonas micra, and the gram-negative anaerobes, porphyromonas gingivalis, prevotella melaninogenica and fusobacterium nucleatum. traditional, as well as more elaborat ...200919459962
molecular analysis of persistent periradicular lesions and root ends reveals a diverse microbial profile.the microbial etiology of periradicular lesions is recognized; however, the bacterial profile of these lesions is not well elucidated. the purpose of this study was to determine the frequency of bacterial colonization in these lesions and to characterize the bacterial community present in root ends and periradicular lesions.200919567313
effect of age on gingival crevicular fluid concentrations of mif and pge2.we used an experimental gingivitis study design to compare crevicular fluid concentrations of migration inhibitory factor (mif) and prostaglandin e(2) (pge(2)) in younger (18 to 30 yrs) and older (46 to 77 yrs) healthy adults. pge(2) increased after 1 wk in younger participants, whereas it decreased in older individuals after 1 wk of plaque accumulation. a significant interaction between age and time was observed for pge(2) (p = 0.04). high concentrations of mif were identified in both age group ...200919641151
bone microbial contamination influences autogenous grafting in sinus augmentation.the oral occurrence of putative microbial pathogens in humans has been documented in health and disease. the presence of periodontopathogens in patients with a history of periodontal disease may have a negative impact on bone regeneration. this investigation was conducted to confirm the presence of periodontal pathogens in bone particles harvested intraorally for maxillary sinus augmentation and to assess the clinical and radiographic outcomes 6 to 12 months after bone augmentation.200919656037
murdochiella asaccharolytica gen. nov., sp. nov., a gram-stain-positive, anaerobic coccus isolated from human wound specimens.two strains of previously unknown gram-stain-positive, anaerobic, coccus-shaped bacteria from human wound specimens were characterized using phenotypic and molecular taxonomic methods. comparative 16s rrna gene sequencing studies and distinguishable biochemical characteristics demonstrated that these two unknown strains, wal 1855c(t) and wal 2038e, are genotypically homogeneous and constitute a novel lineage within clostridium cluster xiii. there was 13-14 % 16s rrna gene sequence divergence bet ...201019666803
comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray.this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim).200919722792
[bacteria isolated from surgical infections and their susceptibility to antimicrobial agents--special reference to bacteria isolated between april 2007 and march 2008].bacteria isolated from infections in abdominal surgery during the period from april 2007 to march 2008 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 707 strains including 24 strains of candida spp. were isolated from 181 (79.0%) of 229 patients with surgical infections. three hundred and ninety-five strains were isolated from primary infections, and 288 strains were isolated from postoperative infections. from primary infections, anae ...200919860320
microbial profile of ligature-induced periodontitis in rats.the aim of this study was to evaluate the composition of the biofilm accumulated around ligatures in rats by checkerboard dna-dna hybridization using probes made from human oral bacterial species.201019931851
the bactericidal effect of a genius nd:yag laser.to evaluate the 'in vitro' bactericidal effect of the nd:yag laser (genius, mølsgaard dental, copenhagen, denmark) on six periodontal pathogens.201020096084
detection of periodontopathogenic bacteria in pregnant women by traditional anaerobic culture method and by a commercial molecular genetic method.to culture facultative and strict anaerobic bacteria is a well-established method for analyzing subgingival plaque samples. micro-ident and micro-ident plus (hain lifescience gmbh, nehren, germany) tests are two commercially available rapid pcr-based methods for the identification and quantification of putative periodontopathogen bacteria. in this study, we compared these commercial pcr-based hybridization methods with conventional anaerobic culture technique. a total of 36 subgingival plaque sa ...201020219690
two new amino-acid-fermenting bacteria, clostridium propionicum and diplococcus glycinophilus. 194620277143
amino acid fermentations by clostridium propionicum and diplococcus glycinophilus. 194720283568
do elderly edentulous patients with a history of periodontitis harbor periodontal pathogens?the presence of periodontal pathogens in the oral cavity may impact implant survival. therefore, this study aimed to determine the prevalence of campylobacter rectus, porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, prevotella intermedia, tannerella forsythia, treponema denticola, eikenella corrodens and parvimonas micra in a specific elderly population with a history of periodontitis who have never worn dentures.201020337663
response of subgingival bacteria to smoking cessation.it has been demonstrated that smoking cessation alters the subgingival microbial profile; however, the response of individual bacteria within this ecosystem has not been well studied. the aim of this investigation, therefore, was to longitudinally examine the effect of smoking cessation on the prevalence and levels of selected subgingival bacteria using molecular approaches for bacterial identification and enumeration. subgingival plaque was collected from 22 smokers at the baseline and 12 month ...201020410352
severe odontogenic infections. epidemiological, microbiological and therapeutic factors.objectives: a retrospective study is made of the odontogenic infections treated in la paz university hospital (madrid, spain) during 2007 and 2008, with an epidemiological and microbiological analysis of a large group of patients. study design: a retrospective descriptive study was made, involving the consecutive inclusion of all patients with odontogenic infection requiring admission to our hospital in the period between january 2007 and december 2008. results: a total of 151 patients were incl ...201020711116
subgingival microbial profiles of smokers with periodontitis.the subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. we tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16s sequencing. smokers ...201020739702
effect of khat chewing on periodontal pathogens in subgingival biofilm from chronic periodontitis patients.existing in vitro and in vivo data suggest that khat may have a favorable effect on periodontal microbiota. the purpose of this study was to assess the effect of khat chewing on major periodontal pathogens in subgingival plaque samples from subjects with chronic periodontitis.201020816745
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents --special references to bacteria isolated between april 2008 and march 2009].bacteria isolated from infections in abdominal surgery during the period from april 2008 to march 2009 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 712 strains including 18 strains of candida spp. were isolated from 173 (80.5%) of 215 patients with surgical infections. three hundred and sixty-six strains were isolated from primary infections, and 346 strains were isolated from postoperative infections. from primary infections, anaero ...201020919496
bactericidal activity of stabilized chlorine dioxide as an endodontic irrigant in a polymicrobial biofilm tooth model system.the purpose of this study was to determine bactericidal efficacy of 0.04% stabilized chlorine dioxide, 3% sodium hypochlorite, 2% chlorhexidine gluconate, and sterile distilled water in a polymicrobial biofilm model.201020951304
setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.caries and periodontitis are important human diseases associated with formation of multi-species biofilms. the involved bacteria are intensively studied to understand the molecular basis of the interactions in such biofilms. this study established a basic in vitro single and mixed-species culture model for oral bacteria combining three complimentary methods. the setup allows a rapid screening for effects in the mutual species interaction. furthermore, it is easy to handle, inexpensive, and repro ...201020957048
early bacterial colonization and soft tissue health around zirconia and titanium abutments: an in vivo study in man.aim: to compare the early bacterial colonization and soft tissue health of mucosa adjacent to zirconia (zro(2) ) and titanium (ti) abutment surfaces in vivo. materials and methods: twenty edentulous subjects received two endosseous mandibular implants. the implants were fitted with either a zro(2) or a ti abutment (non-submerged implant placement, within-subject comparison, left-right randomization). sulcular bacterial sampling and the assessment of probing pocket depth, recession and bleeding o ...201021054554
antimicrobial susceptibility of clinically relevant gram-positive anaerobic cocci collected over a three-year period in the netherlands.the susceptibility of 14 species of 115 gram-positive anaerobic cocci (gpac) was determined for 14 antibiotics. to assure correct identification, strains were genotypically identified by fluorescence in situ hybridization and sequencing. susceptibility differences (mic₅₀ and mic₉₀) for penicillin g, clindamycin, tigecycline, levofloxacin, amoxicillin-clavulanic acid, cefoxitin, ertapenem, meropenem, metronidazole, and doxycycline were found for the three clinically most relevant gpac species: fi ...201021189338
antimicrobial activity of diterpenes from viguiera arenaria against endodontic bacteria.six pimarane-type diterpenes isolated from viguiera arenaria baker and two semi-synthetic derivatives were evaluated in vitro against a panel of representative microorganisms responsible for dental root canal infections. the microdilution method was used for the determination of the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) against porphyromonas gingivalis, prevotella nigrescens, prevotella intermedia, prevotella buccae, fusobacterium nucleatum, bacteroi ...201121233793
tongue biofilm areal density and tongue coating index.two methods have been employed to measure the amount of tongue biofilm in humans: (1) areal density of microbes (cfu cm(-2)) (tongue scrape from a known/measured area), or (2) a tongue-coating index, where a human judge assesses the amount of 'coating' by visual inspection. the two methods were compared, both on human volunteers and on artificial biofilms. fusobacterium nucleatum (fn), prevotella intermedia (pi), streptococcus mutans (sm) and peptostreptococcus micros (pm) were obtained from sto ...200821386152
antibiotic susceptibility of cocultures in polymicrobial infections such as peri-implantitis or periodontitis: an in vitro model.background: although polymicrobial infections, such as peri-implantitis or periodontitis, were postulated in the literature to be caused by synergistic effects of bacteria, these effects remain unclear looking at antibiotic susceptibility. the aim of this study is to compare the antibiotic susceptibilities of pure cultures and definite cocultures. methods: laboratory strains of aggregatibacter actinomycetemcomitans (aa) (previously actinobacillus actinomycetemcomitans), capnocytophaga ochracea ( ...201121405933
clinical periodontal and microbiologic parameters in patients with rheumatoid arthritis.background: a limited number of studies suggest the prevalence of periodontal pathogens in patients with rheumatoid arthritis (ra); however, results are inconsistent. the aim of this study was to investigate clinical periodontal and microbiologic parameters in patients with ra. methods: 66 patients with ra aged 49.5±8.4 years participated in the study. the periodontal situation was assessed with the periodontal screening index (psr(®)/psi); allocated to: healthy, gingivitis (psr(®)/psi score 0-2 ...201121405936
clustering of subgingival microbial species in adolescents with periodontitis.it has become increasingly recognized that groups of microorganisms interact within the subgingival plaque of adult subjects with periodontitis. it is much less clear, however, whether the consortia of microorganisms associated with periodontitis are different in early and more advanced cases of periodontitis. to investigate this point further, subgingival plaque was collected from six sites in 87 adolescents with periodontitis and 73 controls and the samples were analyzed for the detection of 1 ...201121410554
influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces in vitro.objectives: the aim of this study was to analyse the influence of the microtopography and hydrophilicity of titanium (ti) substrates on initial oral biofilm formation. materials and methods: nine bacterial species belonging to the normal oral microbiota, including: aggregatibacter actinomycetemcomitans, actinomyces israelii, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, parvimonas micra, porphyromonas gingivalis, prevotella intermedia, and streptococcus sanguinis were teste ...201121492236
analysis of microbiota associated with peri-implantitis using 16s rrna gene clone library.peri-implantitis (pi) is an inflammatory disease which leads to the destruction of soft and hard tissues around osseointegrated implants. the subgingival microbiota appears to be responsible for peri-implant lesions and although the complexity of the microbiota has been reported in pi, the microbiota responsible for pi has not been identified.201021523229
spiramycin resistance in human periodontitis microbiota.purpose: the occurrence of in vitro resistance to therapeutic concentrations of spiramycin, amoxicillin, and metronidazole was determined for putative periodontal pathogens isolated in the united states. materials and methods: subgingival plaque specimens from 37 consecutive adults with untreated severe periodontitis were anaerobically cultured, and isolated putative periodontal pathogens were identified to a species level. in vitro resistance to spiramycin at 4 μg/ml, amoxicillin at 8 μg/ml, an ...201121524712
effectiveness of ozone against periodontal pathogenic microorganisms.huth kc, quirling m, lenzke s, paschos e, kamereck k, brand k, hickel r, ilie n. effectiveness of ozone against periodontal pathogenic microorganisms. eur j oral sci 2011; 119: 204-210. ©2011 eur j oral sci ozone has been proposed as an adjunct antiseptic in periodontitis therapy. the aim of this study was to investigate the antimicrobial effectiveness of gaseous/aqueous ozone, in comparison with that of the established antiseptic chlorhexidine digluconate (chx), against periodontal microorganis ...201121564314
detection of nine microorganisms from the initial carious root lesions using a taqman-based real-time pcr.oral diseases (2011) objective:  the purpose of this study was to quantify nine selected cariogenic bacteria in plaque from sound root surfaces and initial carious root lesions using taqman pcr and to analyse a putative dependence on the kind of initial periodontal treatment. material and methods:  fifty-four subjects with generalized chronic periodontitis were randomly allocated to one of the following initial periodontal therapies: full-mouth disinfection, full-mouth scaling and root planing o ...201121605286
Tongue piercing: the effect of material on microbiological findings.Biofilms on oral piercings may serve as a bacterial reservoir and lead to systemic bacteremia or local transmission of pathogenic microbiota. The use of piercing materials which are less susceptible to biofilm accumulation could contribute to prevention of problems. The present study investigated whether there are microbiological differences in bacterial samples collected from tongue piercings made of different materials.201121700161
in vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers.the aim of this study was to investigate in vitro the cytotoxicity and antibacterial properties of four different endodontic sealers using human periodontal ligament fibroblast cell proliferation and visual analysis of growth inhibition.201121831282
analysis of symptomatic and asymptomatic primary root canal infections in adult norwegian patients.this molecular study analyzed the microbiota of primary root canal infections from adult norwegian patients.201121846535
[Bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between April 2009 and March 2010].Bacteria isolated from surgical infections during the period from April 2009 to March 2010 were investigated in a multicenter study in Japan, and the following results were obtained. In this series, 671 strains including 16 strains of Candida spp. were isolated from 174 (79.1%) of 220 patients with surgical infections. Four hundred and eleven strains were isolated from primary infections, and 244 strains were isolated from surgical site infection. From primary infections, anaerobic Gram-negative ...201121861307
antibiotic effects on bacterial profile in osteonecrosis of the jaw.oral diseases (2011) 18, 85-95 objective:  oral infection is considered to play a critical role in the pathogenesis of bisphosphonate-related osteonecrosis of the jaw (bronj), and antibiotic therapy has become a mainstay of bronj therapy. this study was aimed to investigate the effect of antibiotics on bacterial diversity in bronj tissues. materials and methods:  the bacterial profile from soft tissues associated with the bronj lesion was determined using 16s rrna-based denaturing gradient gel e ...201221883710
[Multiple complications of acute unilateral rhinosinusitis--a case report].Intracranial or orbital complications after chronic and acute rhinosinusitis are rare, but could be dangerous. The complications of acute rhinosinusitis more often are observed in young patients. The combination of both intracranial and orbital complications together is found after acute inflammation.201121916225
detection of periodontal pathogens in newborns and children with mixed dentition.we report the age-related prevalence of red complex periodontal pathogens, porphyromonas gingivalis, treponema denticola, and tannerella forsythia, along with four strains of orange complex pathogens. the bacteria present in samples isolated from tongue, cheek, and subgingival sulcus in edentulous newborns and children with mixed dentition were monitored by polymerase chain reaction (pcr). p. gingivalis was not detected in any site of any subject in the two groups tested. however, t. denticola w ...201121928086
investigation of six selected bacterial species in endo-periodontal lesions.didilescu ac, rusu d, anghel a, nica l, iliescu a, greabu m, bancescu g, stratul si. investigation of six selected bacterial species in endo-periodontal lesions. international endodontic journal, 45, 282-293, 2012. abstract: aim  to investigate and determine possible associations of six tested bacteria belonging to 'orange' and 'green' complexes, in endo-periodontal lesions: parvimonas micra, fusobacterium nucleatum, campylobacter rectus, eubacterium nodatum, eikenella corrodens and capnocytopha ...201122077868
Persistent extraradicular infection in root-filled asymptomatic human tooth: scanning electron microscopic analysis and microbial investigation after apical microsurgery.Procedural accidents have a negative effect on healing and might contribute to the persistence of infections in inaccessible apical areas, requiring surgical intervention. This report describes a case of persistent apical periodontitis of a lower left first molar associated with the sinus tract and a periapical lesion that required nonsurgical endodontic retreatment and apical surgery for resolution.201122099908
the subgingival microbiota of papillon-lefèvre syndrome.background: there is little information about the microbiological profiles of periodontal lesions in papillon-lefèvre syndrome (pls) and the significance of bacteria in the pathogenesis of periodontitis in these patients. this comprehensive analysis of the subgingival microbiota in pls patients used 16s rrna clonal analysis and the 16s rrna-based human oral microbe identification microarray (homim). methods: thirteen pls patients, from 7 unrelated families, volunteered for this microbiological s ...201122141356
extracellular deoxyribonuclease production by periodontal bacteria.palmer lj, chapple ilc, wright hj, roberts a, cooper pr. extracellular deoxyribonuclease production by periodontal bacteria. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01451.x © 2011 john wiley & sons a/s background and objective:  whilst certain bacteria have long been known to secrete extracellular deoxyribonuclease (dnase), the purpose in microbial physiology was unclear. recently, however, this enzyme has been demonstrated to confer enhanced virulence, enabling bacteria to evade the ...201122150619
gingival fluid cytokine expression and subgingival bacterial counts during pregnancy and postpartum: a case series.the aim of this study was to assess gingival fluid (gcf) cytokine messenger rna (mrna) levels, subgingival bacteria, and clinical periodontal conditions during a normal pregnancy to postpartum.201322249562
necrotizing periodontal diseases in hiv-infected brazilian patients: a clinical and microbiologic descriptive study.objective: nowadays, necrotizing periodontal diseases have a low prevalence; however, a better understanding of the etiopathogenesis of these diseases is necessary for determining more adequate preventive and therapeutic strategies. method and materials: from a pool of 1,232 hiv-infected patients, 15 presented with necrotizing periodontal diseases, which were evaluated by full-mouth periodontal clinical measurements. subgingival biofilm samples were collected from necrotizing lesions of six of t ...201222259811
treponema denticola chymotrypsin-like proteinase (ctlp) integrates spirochaetes within oral microbial communities.treponema denticola is found ubiquitously in the human oral cavity and is mainly associated with bacterial communities implicated in the establishment and development of periodontal disease. the ability to become integrated within biofilm communities is crucial to the growth and survival of oral bacteria, and involves inter-bacterial coaggregation, metabolic cooperation, and synergy against host defences. in this article we show that the chymotrypsin-like proteinase (ctlp), found within a high-m ...201222313692
impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and individuals with refractory periodontitis using the human oral microbe identification microarray.this study compares the changes to the subgingival microbiota of individuals with "refractory" periodontitis (rp) or treatable periodontitis (good responders [gr]) before and after periodontal therapy by using the human oral microbe identification microarray (homim) analysis.201222324467
molecular methods for diagnosis of odontogenic infections.historically, the identification of microorganisms has been limited to species that could be cultured in the microbiology laboratory. the purpose of the present study was to apply molecular techniques to identify microorganisms in orofacial odontogenic infections (ois).201222326175
microbiologic profile of endodontic infections from hiv- and hiv+ patients using multiple-displacement amplification and checkerboard dna-dna hybridization.to compare the microbiota of endodontic infections in necrotic pulp from hiv-negative and hiv-positive subjects.201222335194
antimicrobial activity of streptococcus salivarius k12 on bacteria involved in oral malodour.to investigate the antimicrobial activity of the bacteriocin-producing strain streptococcus salivarius k12 against several bacteria involved in halitosis.201222405584
oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients.oral mucositis is a serious and debilitating side effect of conditioning regimens for hematopoietic stem cell transplant (hsct). through hsct, the homeostasis in the oral cavity is disrupted. the contribution of the oral microflora to mucositis remains to be clarified. the aim of our study was to investigate the relationship between yeasts, bacteria associated with periodontitis, and oral ulcerations in hsct recipients.201222531876
complication of etanercept treatment for rheumatoid arthritis--purulent pericarditis caused by a commensal organism.the patient presented with increasing fatigue and dyspnoea. the patient had medical history of rheumatoid arthritis for which she had been taking methotrexate for the past 15 years and etanercept for the past 6 years. initial diagnosis was cardiac failure but further investigation by echocardiogram revealed a large pericardial effusion. empirical piperacillin-tazobactam was started due to moderately raised inflammatory markers. four hundred millilitre of frank pus was aspirated from the pericard ...201222605835
microbial diversity in failed endodontic root-filled teeth.persistent/secondary infections of human root canals play an important role in the failure of endodontic treatment. this study used 16s rrna sequencing to assess microbial diversity in root-filled teeth associated with failed endodontic treatment.201222613548
microbiological characterization in children with aggressive periodontitis.the objective of this study was to characterize the subgingival microbiota of african-american children with localized aggressive periodontitis (lap). fifty-one children were included. subgingival plaque samples were taken from diseased (dd) and healthy sites (dh) in lap and from healthy sites in hs and hc and analyzed by 16s rrna-based microarrays. aggregatibacter actinomycetemcomitans (aa) was the only species found to be both more prevalent (or = 8.3, p = 0.0025) and abundant (p < 0.01) in dd ...201222863892
long-term follow-up of turned single implants placed in periodontally healthy patients after 16 to 22 years: microbiologic outcome.survival rates in implant dentistry today are high, although late failures do occur for many reasons, including peri-implant infections. the primary objective of this study is to investigate microbiota around single turned implants after 16 to 22 years. secondary objectives are to compare teeth and implants and to correlate microbiologic, radiographic, and clinical parameters.201322934839
do elderly edentulous patients with a history of periodontitis harbor periodontal pathogens?the presence of periodontal pathogens in the oral cavity may impact implant survival. therefore, this study aimed to determine the prevalence of campylobacter rectus, porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, prevotella intermedia, tannerella forsythia, treponema denticola, eikenella corrodens, and parvimonas micra in a specific elderly population with a history of periodontitis who have never worn dentures.201222988660
a double-blind randomized placebo-controlled study on the clinical and microbial effects of an essential oil mouth rinse used by patients in supportive periodontal care.this 3-month double-blind randomized placebo-controlled study evaluated the clinical and microbial effects of an essential oil mouth rinse used as an adjunct to mechanical plaque control by patients in supportive periodontal care.201322998386
subgingival microflora in inflammatory bowel disease patients with untreated periodontitis.to analyze the subgingival microflora composition of inflammatory bowel disease (ibd) patients with untreated chronic periodontitis and compare them with systemically healthy controls also having untreated chronic periodontitis.201323060013
antibacterial activity of calcium hydroxide and chlorhexidine containing points against fusobacterium nucleatum and parvimonas micra.up to 90% of the flora of an infected root canal consists of obligate anaerobic bacteria. however, most studies have centred on microaerophiles. this quantitative assay investigated the antibacterial effects against fusobacterium nucleatum and parvimonas micra of gutta-percha (gp) points containing either calcium hydroxide (ca(oh)(2)) or chlorhexidine (chx) compared to those of conventional gp points.201223077425
microbiological profile and antimicrobial susceptibility pattern of infected root canals associated with periapical abscesses.the aim of this investigation was to identify microorganisms from root canals with periapical abscesses and assess the susceptibility of specific anaerobic bacteria to selected antimicrobials and their β-lactamase production. sixty root canals were microbiologically investigated. the susceptibility of anaerococcus prevotii, fusobacterium necrophorum, f. nucleatum, parvimonas micra, and prevotella intermedia/nigrescens to antimicrobials was evaluated with the etest, whereas β-lactamase production ...201323224675
comprehensive microbiological findings in peri-implantitis and periodontitis.the microbial differences between peri-implantitis and periodontitis in the same subjects were examined using 16s rrna gene clone library analysis and real-time polymerase chain reaction.201323294017
[expression of cytokines in dentogingival sulcus in patients after dental implantation and during development of peri-implantitis].determination of cytokine content in various areas of dentition in patients with peri-implantitis associated with parodontopathogenic bacteria species of i and ii order.201223297645
effectiveness of three antimicrobial mouthrinses on the disinfection of toothbrushes stored in closed containers: a randomized clinical investigation by dna checkerboard and culture.the aim of this in vivo study was to evaluate the efficacy of three antimicrobial solutions on the disinfection of toothbrushes after storage in closed containers.201423316683
clinical and microbiological results following nonsurgical periodontal therapy with or without local administration of piperacillin/tazobactam.we assessed if adjunct administration of piperacillin/tazobactam added clinical and microbiological treatment benefits.201323334268
an investigation of the presence of specific anaerobic species in necrotic primary teeth.different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. the aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (pcs) and root canals (rcs) of infected primary teeth using pcr methods. paired pc and rc samples were collected from 15 subjects and analyzed by pcr for the presence of filifactor alocis, fusobacterium nucleatum, parvimonas micra, porphyromonas endod ...201323538426
comparative evaluation of subgingival plaque microflora in pregnant and non-pregnant women: a clinical and microbiologic study.gingival changes in pregnancy have been attributed to changes in the subgingival biofilm related to hormonal variations.201323633772
effect of metalloporphyrins on red autofluorescence from oral bacteria.the aim of this study was to assess the red autofluorescence from bacterial species related to dental caries and periodontitis in the presence of different nutrients in the growth medium. bacteria were grown anaerobically on tryptic soy agar (tsa) supplemented with nutrients, including magnesium-porphyrins from spinach and iron-porphyrins from heme. the autofluorescence was then assessed at 405 nm excitation. on the tsa without additives, no autofluorescence was observed from any of the species ...201323659237
a consortium of aggregatibacter actinomycetemcomitans, streptococcus parasanguinis, and filifactor alocis is present in sites prior to bone loss in a longitudinal study of localized aggressive periodontitis.aggregatibacter actinomycetemcomitans-induced localized aggressive periodontitis (lap) in african-american adolescents has been documented but is poorly understood. two thousand fifty-eight adolescents aged 11 to 17 years were screened for their periodontal status and the presence of a. actinomycetemcomitans in their oral cavity. seventy-one a. actinomycetemcomitans-negative and 63 a. actinomycetemcomitans-positive periodontally healthy subjects were enrolled, sampled, examined, and radiographed ...201323784124
16s rrna region based pcr protocol for identification and subtyping of parvimonas micra.the present study established a pcr protocol in order to identify parvimonas micra and to evaluate the intra-species diversity by pcr-rflp of 16s rrna partial sequence. the data indicated that the protocol was able to identify this species which could be clustered in five genotypes.200824031274
binding properties of treponema denticola lipooligosaccharide.the cell-surface lipooligosaccharide (los) of treponema denticola possesses several biological properties. the aim of this study was to investigate the binding properties of t. denticola los to extracellular matrix (ecm) proteins, mucosal cells, and oral bacteria.201324049558
microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis.the detection of special bacterial species in patients with periodontitis is considered to be useful for clinical diagnosis and treatment. the collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. however, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria. the aim of this cross-sectional s ...201424144271
analysis of bacterial flora associated with peri-implantitis using obligate anaerobic culture technique and 16s rdna gene sequence.to analyze and characterize the predominant bacterial flora associated with peri-implantitis by using culture techniques under obligate anaerobic conditions and 16s rdna gene sequences.201324278920
differences in bacterial saliva profile between periodontitis patients and a control cohort.periodontitis is a multifactorial disease in which subgingival bacteria play an important role in the pathogenesis of the disease. the objective of this study was to determine if periodontitis is associated with a characteristic salivary bacterial profile. this was accomplished by comparing the bacterial profile of saliva from subjects with chronic periodontitis with that of saliva from a control cohort.201424303924
genome sequence of parvimonas micra strain a293, isolated from an abdominal abscess from a patient in the united kingdom.parvimonas micra is an important oral microbe that has the ability to grow and proliferate within oral biofilms and is involved in periodontal disease, leading to gingival bleeding, gingival recession, alveolar bone loss, and tooth mobility. however, occasionally these normally oral pathogens can cause infections at other sites in the body. we present the genome sequence of parvimonas micra strain a293, a smooth parvimonas micra strain isolated from an abdominal abscess from a patient at barts h ...201324309744
multifocal hematogenous vertebral osteomyelitis due to parvimonas micra and a subsequent pleural effusion in a diabetic patient. 201424315516
the effects of different orthodontic appliances upon microbial communities.orthodontic appliances can promote accumulation of dental plaque, with associated enamel decalcification or gingival inflammation. the aim of this study was to examine longer-term microbiological changes during orthodontic treatment with fixed appliances.201424345204
complications of adenotonsillectomy: a case report of meningitis due to dual infection with nontypeable haemophilus influenzae and streptococcus pneumoniae, and a prospective study of the rate of postoperative bacteremia.bacterial meningitis is a rare complication of adenotonsillectomy. we present a case of meningitis due to nontypeable haemophilus influenzae and streptococcus pneumoniae after adenotonsillectomy. pulsed-field gel electrophoresis patterns indicated that the oral cavity was the source of h. influenzae and s. pneumoniae isolated from the cerebrospinal fluid. blood culture study: as bacteremia is thought to be one of the etiologies of meningitis, we prospectively investigated the rate of bacteremia ...201324396981
performance evaluation and bacteria analysis of afb-mfc enriched with high-strength synthetic wastewater.in order to study the performance and bacterial communities of an anaerobic fluidized bed microbial fuel cell (afb-mfc) system, the 16s rdna gene sequencing was applied, and high-strength synthetic wastewater was treated by the afb-mfc system. the high-strength synthetic wastewater, in which the concentrations of chemical oxygen demand (cod), nitrite nitrogen, and nitrate nitrogen were above 19,000, 2,516-3,871 and 927-1,427 mg/l, was treated by the afb-mfc system. the removal efficiency of cod, ...201424434962
preservation of bacterial dna by human dentin.the capacity of dentin and collagen to bind dna and protect against spontaneous and nuclease-induced degradation was evaluated individually and by the incubation of dna with nuclease-producing bacteria in a mixed culture.201424461411
[a case of septic pulmonary embolism due to peptostreptococcus micros with multiple infection of the head and neck].a 46-year-old man with no underlying diseases visited our hospital with otorrhea, ocular motility disorder of the left eye, dizziness and loss of appetite which had lasted for two months. chest radiography and computed tomography (ct) showed bilateral multiple pulmonary nodules and cavities. furthermore, ct of the head and neck revealed bilateral mastoiditis, a left orbital abscess and a deep neck abscess. peptostreptococcus micros was cultured from blood and otorrhea specimens. in addition, p. ...201324483025
bacteremia following scaling and root planing: a clinico-microbiological study.bacteremia frequently occurs after treatment procedures such as extractions, scaling, root planing, periodontal surgery. there is currently significant interest in the possibility that bacteremia with oral bacteria may play role in pathogenesis of atherosclerosis. there are well-conducted studies that have determined the frequency of passage of periodontal microorganisms to the bloodstream after periodontal treatment. there is scarce information related to the incidence of periodontopathic micro ...201324554880
beta-lactamic resistance profiles in porphyromonas, prevotella, and parvimonas species isolated from acute endodontic infections.susceptibility to beta-lactamic agents has changed among anaerobic isolates from acute endodontic infections. the aim of the present study was to determine the prevalence of the cfxa/cfxa2 gene in prevotella spp., porphyromonas spp., and parviomonas micra strains and show its phenotypic expression.201424565649
bacteria community study of combined periodontal-endodontic lesions using denaturing gradient gel electrophoresis and sequencing analysis.the entire microbial population and predominant microflora of root canals (rcs) and adjacent periodontal pockets (pps) from teeth with combined periodontal-endodontic lesions were determined and compared.201424579762
microbiological assessment of the implant-abutment interface in different connections: cross-sectional study after 5 years of functional loading.to evaluate the bacterial microflora present inside the implant connection and in the peri-implant sulcus fluid of healthy implants, and to analyze the relationships between these harboring sites for four different implant systems after at least 5 years of functional loading.201524665919
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