Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [the isolation of mutant salmonella cholerae-suis with the cya and crp phenotypes and a study of their biological properties]. | 1994 | 7879548 | |
| evidence for functional polymorphism of the spvr gene regulating virulence gene expression in salmonella. | the expression of salmonella enterica spv virulence genes was studied in serovariants dublin and typhimurium using western blotting (immunoblotting), spv-lacz operon fusions and northern blotting. the spva protein was detected in immunoblots from stationary phase cultures of dublin but not from the corresponding cultures of typhimurium. transcriptional measurements, using a spva-lacz operon fusion, indicated 8-10 times higher spva transcription in dublin. in an isogenic escherichia coli chromoso ... | 1995 | 7891657 |
| cloning and molecular analysis of the salmonella enterica ansp gene, encoding an l-asparagine permease. | a gene (ansp), which encodes an l-asparagine permease, has been isolated from a cosmid library of salmonella enterica during screening for recombinant clones which encode l-asparaginase. nucleotide sequence analysis reveals that the gene product is a polypeptide of 497 amino acid residues, containing 12 putative transmembrane segments. the calculated molecular mass is 54 kda, although maxicell analysis by sds-page gave an apparent molecular mass of 37 kda. comparison of the deduced amino acid se ... | 1995 | 7894705 |
| clonal lines of salmonella enterica serotype enteritidis documented by is200-, ribo-, pulsed-field gel electrophoresis and rflp typing. | sixty-two selected strains of salmonella serotype enteritidis of 33 phage types (pts), and one strain classified as rdnc, were characterised by four different chromosomally based typing methods to elucidate genetic relationships among strains of different phage types. based on is200-hybridisation patterns, two major groups, containing strains of the most commonly encountered phage types, and six minor groups (seven with the rdnc strain included) were observed. is200 pattern was a stable epidemio ... | 1994 | 7904649 |
| immersion disinfection of irreversible hydrocolloid impressions with sodium hypochlorite. part i: microbiology. | current american dental association infection control guidelines recommend immersion disinfection of irreversible hydrocolloid impressions, and this study further defines the parameters for use of sodium hypochlorite. sodium hypochlorite has been shown to be an effective disinfectant for impressions; however, it has not been fully evaluated for optimum immersion time and concentration. in this study, irreversible hydrocolloid impressions contaminated with different bacteria were immersed in vary ... | 1994 | 7916888 |
| salmonella typhimurium phage types from human salmonellosis in denmark 1988-1993. | a total of 989 isolates of salmonella enterica ssp. enterica serovar typhimurium from cases of human salmonellosis were investigated by phage typing. the isolates comprised all isolates recovered during the month of august in each of the years from 1988 to 1993. phage typing assigned 82.6% of the strains to 36 different definitive types, 11.9% of the strains belonged to types of unknown lysis pattern (rdnc), and 5.5% could not be typed by the phages used (nt). three phage types (12, 66 and 110) ... | 1994 | 7917221 |
| intergeneric transfer and recombination of the 6-phosphogluconate dehydrogenase gene (gnd) in enteric bacteria. | the gnd gene, encoding 6-phosphogluconate dehydrogenase (ec 1.1.1.44), was sequenced in 87 strains of 15 species assigned to five nominal genera of the enterobacteriaceae, including 36 isolates of salmonella enterica and 32 strains of escherichia coli. in s. enterica, the effective (realized) rate of recombination of horizontally transferred gnd sequences is only moderately higher than the rates for other chromosomal housekeeping genes. in contrast, recombination at gnd has occurred with such hi ... | 1994 | 7937867 |
| genomic lineage of salmonella enterica serovar dublin. | thirty five strains of the host adapted salmonella serotype dublin (s. dublin) have been characterized by is200 patterns, ribotyping, pulsed-field gel electrophoresis (pfge), restriction fragment polymorphism after hybridization with five randomly cloned dna-fragments of s. enteritidis (rflp), and plasmid profiling in order to divide the strains into 'genomic lines'. for comparison, 20 other strains of 9 different group-d serotypes were included. the is200 patterns were identical in all strains ... | 1994 | 7941292 |
| in vitro synthesis of cdp-d-abequose using salmonella enzymes of cloned rfb genes. production of cdp-6-deoxy-d-xylo-4-hexulose, cdp-3,6-dideoxy-d-xylo-4-hexulose and cdp-3,6-dideoxy-d-galactose, and isolation by hplc. | in vitro enzymic synthesis of cdp-d-[u-14c]abequose, cdp-6-deoxy-d-xylo-4-hexulose and cdp-3,6-dideoxy-d-xylo-4-hexulose was achieved using enzymes from cell extracts of cultures of escherichia coli strains harbouring and expressing genes of the rfb gene cluster of salmonella enterica lt2. from an initial synthesis step, cdp-6-deoxy-d-xylo-4-hexulose was isolated after 30 min reaction, using cdp-d-glucose, nad and cdp-glucose 4.6-dehydratase, followed by protein precipitation and desalting by ge ... | 1994 | 7957223 |
| epidemic dissemination of salmonella enterica spp. enterica serovar bovismorbificans in southern italy in the years 1989-1991. | epidemic strains of salmonella enterica subsp. enterica serovar bovismorbificans isolated in southern italy during the years 1989-1991 were submitted to a molecular epidemiological study in comparison with isolates identified in the years 1980-1988 in the same geographic area. genomic dna fragments obtained by digestion with bgli or eco ri hybridized with escherichia coli rrna to produce three distinct, but highly related patterns. ribotype 1, which had never been identified before 1989, was fou ... | 1994 | 7957796 |
| transcriptional regulation of salmonella enterica virulence plasmid genes in cultured macrophages. | the plasmid-carried spv genes promote virulence of salmonellae in mice by allowing bacterial growth in the reticuloendothelial tissue. when the bacteria are cultivated under normal laboratory conditions the spv genes appear dormant. this communication explores the transcriptional regulation of spv genes within murine macrophage-like j774-a.1 cells utilizing a new reporter system. transcriptional fusions were constructed between promoter elements of the salmonella enterica var. typhimurium spv ge ... | 1993 | 7968517 |
| reactive arthritis following an outbreak of salmonella infection in finland. | the incidence and clinical features of reactive arthritis (rea) following an outbreak of enteritis caused by salmonella enterica ssp. enterica serovar 4,5,12:b:-were studied in the autumn of 1992. the outbreak occurred in several municipalities and originated from sprouted mung beans. a questionnaire on extra-enteric manifestations such as joint and eye symptoms was mailed 2 to 5 months after the outbreak to all bacteriologically proven cases. two hundred and forty-six of the 272 (90%) subjects ... | 1994 | 8000742 |
| molecular analysis of the rfb gene cluster of a group d2 salmonella enterica strain: evidence for its origin from an insertion sequence-mediated recombination event between group e and d1 strains. | the salmonella enterica o antigen is a highly variable surface polysaccharide composed of a repeated oligosaccharide (the o unit). the o unit produced by serogroup d2 has structural features in common with those of groups d1 and e1, and hybridization studies had previously suggested that the d2 rfb gene cluster responsible for o-unit biosynthesis is indeed a hybrid of the two. in this study, the rfb gene cluster was cloned from a group d2 strain of s. enterica sv. strasbourg. mapping, hybridizat ... | 1994 | 8021222 |
| isolations of salmonella choleraesuis var kunzendorf from swine herds in southwestern ontario (1980-1993). | 1994 | 8050078 | |
| regulation of spvr gene expression of salmonella virulence plasmid pkdsc50 in salmonella choleraesuis serovar choleraesuis. | the expression regulation of spvr, a regulatory gene on the virulence plasmid (pkdsc50) of salmonella choleraesuis serovar choleraesuis, was investigated by spvr-lacz translational fusion. the spvr gene was found to be positively regulated by its own product, the spvr protein, and this unusual positive autoregulation was repressed by the products of spva and spvb, virulence-associated genes present downstream from the spvr gene. amino acid sequence analysis revealed that the amino-terminal regio ... | 1994 | 8052129 |
| in vitro adhesion and invasion of salmonella enterica serovar havana. | a total of 132 isolates of the havana serovar of salmonela enterica were studied for their adhesion to and invasion of hela cells in the presence of d-mannose. a total of 124 (93.9%) isolates were adherent, and 51% of these were invasive. of these 124 strains 82 (66%) adhered to hela cells with a pattern typical for enteroaggregative (eagg) escherichia coli whereas the remaining 34% adhered in a non-aggregative (non-eagg) pattern. of the 82 strains showing the eagg pattern 60% were invasive wher ... | 1994 | 8057827 |
| increased occurrence of kcn-resistant salmonella strains. | 104 kcn-resistant salmonella strains were detected among 1391 strains typed (7.4%) during the first 7 months in 1993. in particular salmonella enterica strains of subspecies i isolated from humans and foods exhibited this character. those kcn-resistant salmonella strains did not belong to a special antigen formula. the second flagellar phase could not be detected in most of these strains. kcn resistance does not seem to be plasmid-encoded. | 1994 | 8061418 |
| studies of the biosynthesis of 3,6-dideoxyhexoses: molecular cloning and characterization of the asc (ascarylose) region from yersinia pseudotuberculosis serogroup va. | the 3,6-dideoxyhexoses are found in the lipopolysaccharides of gram-negative bacteria, where they have been shown to be the dominant antigenic determinants. of the five 3,6-dideoxyhexoses known to occur naturally, four have been found in various strains of salmonella enterica (abequose, tyvelose, paratose, and colitose) and all five, including ascarylose, are present among the serotypes of yersinia pseudotuberculosis. although there exists one report of the cloning of the rfb region harboring th ... | 1994 | 8071227 |
| basement membrane carbohydrate as a target for bacterial adhesion: binding of type i fimbriae of salmonella enterica and escherichia coli to laminin. | adherence of type-1-fimbriate salmonella enterica and escherichia coli to immobilized proteins of the extracellular matrix and reconstituted basement membranes was studied. the type-1-fimbriate strain sh401 of s. enterica serovar enteritidis showed good adherence to laminin, whereas the adherence to fibronectin, type i, type iii, type iv or type v collagens was poor. only minimal adherence to the matrix proteins was seen with a non-fimbriate strain of s. enterica serovar typhimurium. a specific ... | 1993 | 8095317 |
| lysogenic phage in salmonella enterica serovar heidelberg (salmonella heidelberg): implications for organism tracing. | a phage typing system using a group of 11 closely related phage (as judged by southern analysis and restriction fragment length polymorphism analysis) was able to distinguish at least six phage types in salmonella heidelberg of human and animal origin. restriction fragment length polymorphism analysis using cosmid probes from s. heidelberg confirmed that most s. heidelberg isolates belong to a single 'clonal' group. southern analysis using dna isolated from each of the testing phage group showed ... | 1993 | 8099890 |
| molecular genetic basis of allelic polymorphism in malate dehydrogenase (mdh) in natural populations of escherichia coli and salmonella enterica. | nucleotide sequences of the mdh gene encoding the metabolic enzyme malate dehydrogenase (mdh) were determined for 44 strains representing the major lineages of escherichia coli and the eight subspecies of salmonella enterica. sequence diversity was four times greater in s. enterica than in e. coli, and in both species the rate of amino acid substitution was lower in the nad(+)-binding domain than in the catalytic domain. divergence of the mdh genes of the two species apparently has not involved ... | 1994 | 8108402 |
| analysis of the antibiotic resistance detected in 2043 strains of salmonella enterica subsp. enterica isolated in stool cultures of spanish patients with acute diarrhoea (1986-1991). | 1993 | 8125841 | |
| antimicrobial susceptibility of actinobacillus pleuropneumoniae, pasteurella multocida and salmonella choleraesuis isolates from pigs. | the in vitro susceptibility of 839 isolates of actinobacillus pleuropneumoniae, 969 isolates of pasteurella multocida and 104 isolates of salmonella choleraesuis from pigs to the fluoroquinolone danofloxacin, and eight other commonly used antimicrobial drugs was determined by veterinary diagnostic laboratories in europe, japan, south africa and north america between 1989 and 1991, by using a broth microdilution technique. the minimum inhibitory concentrations of danofloxacin, amoxycillin, ceftio ... | 1994 | 8128570 |
| intermediate filament-like network formed in vitro by a bacterial coiled coil protein. | the tlpa protein encoded by the virulence plasmid of salmonella enterica is an alpha-helical 371-amino acid protein possessing characteristics similar to eukaryotic coiled coil proteins (koski, p., saarilahti, h., sukupolvi, s., taira, s., rikkonen, p., osterlund, k., hurme, r., and rhen, m. (1992) j. biol. chem. 267, 12258-12265). in this paper we have investigated inter- and intramolecular associations and the morphology of structures formed by tlpa. dynamics and temperature stability of tlpa ... | 1994 | 8144657 |
| recombinational basis of serovar diversity in salmonella enterica. | the flic gene, which encodes phase 1 flagellin, was sequenced in strains of 15 salmonella enterica serovars expressing flagellar antigenic factors of the g series. the occurrence of each of the flagellin serotypes g,m, m,t, and g,z51 in distantly related strains is the result of horizontal exchange of dna, as indicated by identity or close similarity in nucleotide sequence of all or parts of the antigenic factor-determining central region of flic. the flagellin genes of some serovars are complex ... | 1994 | 8146152 |
| photochemical inactivation of pathogenic bacteria in human platelet concentrates. | platelet concentrates (pc) may be infrequently contaminated with low levels of bacteria that can cause septicemia and death in patients receiving transfusion therapy. we evaluated the efficacy of a photochemical decontamination (pcd) technique using 8-methoxypsoralen (8-mop) and long wavelength uv light (uva) to inactivate bacteria in standard therapeutic pc. twelve phylogenetically distinct pathogenic bacteria, 5 gram-positive and 7 gram-negative organisms, were seeded into pc to a final challe ... | 1994 | 8167348 |
| characterization of the dtdp-rhamnose biosynthetic genes encoded in the rfb locus of shigella flexneri. | the nucleotide sequence of the proximal half of the rfb region of shigella flexneri has been determined, and the genes encoding enzymes involved in the biosynthesis of dtdp-rhamnose have been identified. these genes show strong homology to the rfb genes encoding dtdp-rhamnose biosynthesis in salmonella enterica serovar typhimurium (strain lt2) and s. enterica serovar anatum (strain m32) (jiang et al., 1991; wang et al., 1992). an open reading frame upstream of rfbb was also identified which enco ... | 1994 | 8170390 |
| the characterization of danish isolates of salmonella enterica serovar enteritidis by phage typing and plasmid profiling: 1980-1990. | plasmid profiling, phage typing and antimicrobial resistance typing have been carried out on 736 isolates of salmonella enterica serovar enteritidis collected in denmark during the period 1980 to 1990. strains originated from cases of human salmonellosis, broiler poultry flocks, layer poultry flocks, quarantined imported poultry, environmental samples from hatchery units, and from bovines. phage type (pt) 1 was found to be the most common type among isolates of poultry origin (57.6%) followed by ... | 1994 | 8185887 |
| sendai virus pneumonia: evidence for the early recruitment of gamma delta t cells during the disease course. | we previously reported that gamma delta t cells appeared and could play a protective role early in infections with intracellular bacteria such as listeria monocytogenes, mycobacterium bovis bcg, and salmonella choleraesuis. to extend these findings to virus infection, we examined the developmental sequence of gamma delta t cells in bronchoalveolar lavage during the course of sendai virus infection in c57bl/6 mice. to produce a natural but nonlethal infection course as far as possible, we used a ... | 1994 | 8189536 |
| [outbreaks of food poisonings of microbial and parasitic origins]. | in order to know the agents and foods related more frequently with food-borne disease outbreaks, we reviewed all the outbreaks studied between 1980 and 1989 by the national laboratory of public health. a total of 79 outbreaks of food-borne diseases of microbial origin were reviewed. the causative agent was identified in 50 (73%) outbreaks. twenty-four per cent of the outbreaks occurred in parties, 10.3 per cent in school or nurseries, 8.6 per cent in restaurants and 8.6 per cent in hospitals. st ... | 1993 | 8235891 |
| identification of six open reading frames in the salmonella enterica subsp. enterica ser. typhi viab locus involved in vi antigen production. | 1993 | 8248629 | |
| short-term treatment of traveler's diarrhea with norfloxacin: a double-blind, placebo-controlled study during two seasons. | clinical efficacy of norfloxacin for treatment of traveler's diarrhea in 106 finnish tourists vacationing in morocco was evaluated during two different seasons. when the criteria for diagnosis of traveler's diarrhea were fulfilled, norfloxacin (400 mg) or a placebo was given orally, twice daily for 3 days. all symptoms and signs subsided sooner in the norfloxacin group. the clearest difference was observed in the duration of diarrhea: 1.2 days in the norfloxacin group vs. 3.3 days in the placebo ... | 1993 | 8268363 |
| purification, characterization, and high performance liquid chromatography assay of salmonella glucose-1-phosphate cytidylyltransferase from the cloned rfbf gene. | we report the purification and characterization of glucose-1-phosphate cytidylyltransferase, the first of five enzymes committed to biosynthesis of cdp-d-abequose from salmonella enterica strain lt2. the purification was greatly facilitated by using a cloned rfbf gene encoding this enzyme. pure enzyme was obtained by 64-fold enrichment in three chromatography steps. the nh2-terminal sequence of the purified enzyme was in agreement with the sequence predicted from the nucleotide sequence of the r ... | 1994 | 8276783 |
| udp-sugar hydrolase isozymes in salmonella enterica and escherichia coli: silent alleles of usha in related strains of group i salmonella isolates, and of ushb in wild-type and k12 strains of e. coli, indicate recent and early silencing events, respectively. | escherichia coli contains a single periplasmic udp-glucose hydrolase (5'-nucleotidase) encoded by usha. salmonella enterica, serotype typhimurium, also contains a single udp-glucose hydrolase but, in contrast to e. coli, it is membrane-bound and is encoded by the non-homologous ushb gene; salmonella enterica (typhimurium) also contains a silent allele of the usha gene (usha0). in this report, we show that nearly all natural isolates of salmonella contain both udp-sugar hydrolases, i.e. they are ... | 1993 | 8288106 |
| acute phase response to salmonella in a pig liver perfusion model. | livers from 7- to 14-day old pigs were maintained on a perfusion apparatus for 3 h. the perfused livers maintained physiologic and immunologic functions during perfusion. the perfused livers retained 78-94% of a non-recirculating inoculum of approximately 1-5 x 10(8) salmonella choleraesuis (scs), and cleared 94.9 +/- 1.7% of the retained (scs) during the 3-h perfusion period. when the acute phase response (apr) was induced in liver donor pigs 24 h before liver perfusion, the perfused livers had ... | 1993 | 8291202 |
| noti macrorestriction analysis suggests a clonal relationship of salmonella enterica, ser. enteritidis phage lysotype 4 strains. | 30 different strains of salmonella enterica ser. enteritidis (s. enteritidis) isolated at the institute for medical microbiology, hannover, were subjected to a noti macrorestriction analysis. the results were compared with the respective phage lysis patterns. 25 of 30 isolates belonged to phage lysotype 4; all these strains displayed an identical genomic noti restriction fragment pattern. in contrast, five isolates of other phage lysotypes revealed more or less prominent differences in their not ... | 1993 | 8300252 |
| characterization of the small cryptic plasmid, pimvs1, of salmonella enterica ser. typhimurium. | the nucleotide sequence has been determined of a small cryptic plasmid of salmonella typhimurium, designated pimvs1, which correlated with an outbreak of gastroenteritis. this plasmid has a size of 3357 bp with no known functions. it does not encode any protein products but shows homology to several well-characterized plasmids. the replication system with rnai and rnaii and the replication origin (oriv) is highly similar to that of plasmid p15a. it also has a putative mobilisation origin similar ... | 1993 | 8302933 |
| presence of microorganisms in used ultrasonic cleaning solutions. | this study analyzed ultrasonic cleaning solutions for bacterial contamination and for antibacterial activity after the solutions were used for a full day in dental offices. ten dental offices ultrasonically cleaned their instruments by their standard procedures for a full day using one batch of their normal cleaning solution. the solutions were then quantitatively analyzed for levels of bacterial contamination and for antibacterial activity against staphylococcus aureus and pseudomonas aeruginos ... | 1993 | 8329158 |
| [bacterial ascites caused by salmonella enterica subsp. enterica serotype indiana]. | 1993 | 8368710 | |
| purification, characterization and hplc assay of salmonella glucose-1-phosphate thymidylyl-transferase from the cloned rfba gene. | we here report on the purification and characterization of glucose-1-phosphate thymidylyl-transferase, the first of four enzymes committed to biosynthesis of dtdp-l-rhamnose from salmonella enterica strain lt2. the purification was greatly facilitated by the cloning of the rfba gene encoding this enzyme. pure enzyme was obtained by 109-fold enrichment in three chromatography steps. the glucose-1-phosphate thymidylytransferase catalyzes a reversible bimolecular group transfer reaction and kinetic ... | 1993 | 8382158 |
| cloning and molecular characterization of a gene involved in salmonella adherence and invasion of cultured epithelial cells. | our laboratories have independently identified a gene in salmonella choleraesuis and salmonella typhimurium that is necessary for efficient adherence and entry of these organisms into cultured epithelial cells. introduction of a mutated gene into several salmonella strains belonging to different serotypes rendered these organisms deficient for adherence and invasion of cultured cells. this effect was most pronounced in the host-adapted serotypes salmonella gallinarum, s. choleraesuis, and salmon ... | 1993 | 8382333 |
| isolation and characterization of carboxylesterase e3 from salmonella enterica. | three esterases (est-) hydrolysing alpha-naphthyl acetate: est-e1, est-e3 and est-e4 produced by salmonella enterica serovar typhimurium, strain lt2 were separated by deae chromatography and gel filtration. est-e3, the major component of this set of enzymes, clearly differed from the two other esterases by its apparent molecular weight, titration curve, substrate specificity and inactivation. immunoglobulins raised against est-e3 completely neutralized the activity of est-e3 but did not react wi ... | 1993 | 8407677 |
| regulation of the ansb gene of salmonella enterica. | the expression of l-asparaginase ii (encoded by ansb) in salmonella enterica was found to be positively regulated by the camp receptor protein (crp) and anaerobiosis. the anaerobic regulation of the s. enterica ansb gene is not mediated by the anaerobic transcriptional activator fnr. this is unlike the situation of the ansb gene of escherichia coli, which is dependent on both crp and fnr. to investigate this fundamental difference in the regulation of l-asparaginase ii expression in s. enterica, ... | 1993 | 8412661 |
| nontoxic forms of lipopolysaccharides, poorly cleared in vivo, are present in variable amounts in phenol-extracted endotoxins. | a radiolabeled lipopolysaccharide (lps) from salmonella choleraesuis was as toxic for galactosamine-treated mice as the unlabeled preparation. after intravenous injection, a large proportion (37%) of the labeled material remained in the circulation. this circulating form of lps was not toxic, even when reextracted with phenol, and remained in the circulation when reinjected into mice. furthermore, toxic and nontoxic constituents were directly isolated in vitro by hydrophobic gel chromatography o ... | 1993 | 8418160 |
| oral immunization of mice and swine with an attenuated salmonella choleraesuis [delta cya-12 delta(crp-cdt)19] mutant containing a recombinant plasmid. | salmonella choleraesuis chi 3781, an attenuated [delta cya-12 delta(crp-cdt)19] mutant, was electroporated with the plasmid pba31-r7, which codes for the expression of a 31-kda protein from brucella abortus (bcsp31). recombinant s. choleraesuis chi 3781 stably maintained the pba31-r7 plasmid and continued to express the cloned protein following recovery of the organism from orally inoculated animals. unlike previous studies using s. typhimurium chi 4064(pba31-r7), s. choleraesuis chi 3781(pba31- ... | 1993 | 8423090 |
| molecular mechanism of the regulation of expression of plasmid-encoded mouse bacteremia (mba) genes in salmonella serovar choleraesuis. | the regulation of mouse bacteremia genes (mba genes) encoded by a 6.4 kb region on the 50 kb virulence plasmid (pkdsc50) of salmonella serovar choleraesuis was analyzed. the genes mba1, mba2, mba3, and mba4, are arranged in this order, and form a cluster located in the 6.4 kb mba region. we prepared four antibodies, each specific for an individual mba protein, using synthetic peptides as antigens. their amino acid sequences were deduced from the dna sequence of the corresponding mba genes. each ... | 1993 | 8437568 |
| molecular analysis of the 3,6-dideoxyhexose pathway genes of yersinia pseudotuberculosis serogroup iia. | salmonella enterica and yersinia pseudotuberculosis are the only examples in nature known to use a variety of 3,6-dideoxyhexose derivatives as o antigen constituents. to allow a comparison of the responsible biosynthetic genes of the two organisms, we have sequenced a section of the y. pseudotuberculosis serogroup iia rfb region that contained the genes for the abequose biosynthetic pathway. comparison of the identified genes with the rfb region of s. enterica lt2 showed that the two dideoxyhexo ... | 1993 | 8444803 |
| salmonella choleraesuis pneumonia in a cat without signs of gastrointestinal tract disease. | primary salmonella choleraesuis pneumonia without enteric manifestations was diagnosed in an 8-year-old cat. the diagnosis was based on culture of the organism from bronchial brushings. pathogens were not isolated from multiple fecal samples. the cat initially responded to treatment with sulfamethoxazole trimethoprim, but relapsed after the owner discontinued treatment because of difficulty in administration. treatment with ampicillin, followed by amoxicillin, resulted in complete resolution of ... | 1993 | 8468222 |
| different appearance of gamma delta t cells during salmonellosis between ityr and itys mice. | ity gene, which is believed to be a single gene on proximal mouse chromosome 1, determines susceptibility to salmonella infection. we have recently demonstrated that the gamma delta t cells specific for purified protein derivative appear at the early stage after infection with salmonella choleraesuis strain 31n-1 (avirulent strain) in mice. in the present study, we compared the appearance of the gamma delta t cells in ityr and itys mice after infection with s. choleraesuis in an attempt to eluci ... | 1993 | 8468480 |
| purification and characterization of three carboxylesterases from enterobacteriaceae. | the carboxylesterases from proteus vulgaris, salmonella enterica and citrobacter amalonaticus were purified 104-, 95- and 120-fold, respectively by chromatography. the enzymes had similar catalytic activities but differed considerably in their inactivation by heat, di-isopropyl fluorophosphate and cd2+, zn2+, hg2+ and cu2+. quantitative neutralization of hydrolytic activity with specific immunoglobulins indicated that the three enzymes were antigenically distinct. | 1993 | 8472928 |
| molecular analysis of spv virulence genes of the salmonella virulence plasmids. | genes on an 8 kb region common to the virulence plasmids of several serovars of salmonella are sufficient to replace the entire plasmid in enabling systemic infection in animal models. this virulence region encompasses five genes which previously have been designated with different names from each investigating laboratory. a common nomenclature has been devised for the five genes, i.e. spv for salmonella plasmid virulence. the first gene, spvr, encodes a positive activator for the following four ... | 1993 | 8483415 |
| [enteric fever caused by multiresistant salmonella typhi: 2 autochthonous cases]. | references about isolation of strains of salmonella typhi multiply resistant to antimicrobials and native from spain are very scant. we describe here two cases of typhoid fever produced by two identical strains of salmonella typhi. both strains are multiresistant and autochthonous, judging from epidemiological investigations. | 1993 | 8512972 |
| tumor necrosis factor-alpha production in swine after oral or respiratory challenge exposure with live salmonella typhimurium or salmonella choleraesuis. | a series of experiments was conducted to document tumor necrosis factor-alpha (tnf) activity in serum of swine after inoculation with salmonella spp endotoxin and after oral or respiratory tract challenge exposure with live salmonella spp. for experiment 1, a potentially lethal dose of s typhimurium endotoxin (25 micrograms/kg of body weight) was administered iv, and serum tnf activity was measured. high tnf (approx 700 iu/ml) activity at 1 to 2 hours after administration of the inoculum was ass ... | 1995 | 8533971 |
| genetic organisation and evolution of yersinia pseudotuberculosis 3,6-dideoxyhexose biosynthetic genes. | 3,6-dideoxyhexose (ddh) sugars occur in some of the o antigens of salmonella enterica and yersinia pseudotuberculosis, but are otherwise rarely found in nature. y. pseudotuberculosis ddh biosynthetic genes rfbs (encoding cdp-paratose synthetase) and rfbe (encoding cdp-tyvelose epimerase) were amplified and cloned, and their sequences determined. comparisons with the equivalent genes of s. enterica show that the genetic arrangement of ddh genes is very similar; however, in y. pseudotuberculosis t ... | 1995 | 8541300 |
| il-15 is a novel growth factor for murine gamma delta t cells induced by salmonella infection. | we have previously shown evidence for the early recruitment of gamma delta t cells during the disease course of primary infections with listeria monocytogenes or salmonella choleraesuis in mice. since gamma delta t cells at this stage of the disease do not produce il-2, the growth factor for the gamma delta t cells remains unknown. il-15 is a novel cytokine that uses beta- and gamma-chain of il-2r for signal transduction, and is produced by activated monocytes/macrophages. in this study, we inve ... | 1996 | 8543818 |
| molecular typing of salmonella enterica subsp. enterica serovar enteritidis isolates. | a collection of 31 epidemiologically unrelated salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) isolates obtained during a 12-year period was characterised by different molecular typing methods. plasmid profile analysis, the detection of plasmid-encoded virulence genes and ribotyping allowed little or no further differentiation amongst these isolates. two different hybridisation patterns were observed by is200-typing of the s. enteritidis isolates. however, pulsed-field g ... | 1996 | 8544212 |
| differentiation of strains from a food-borne outbreak of salmonella enterica by phenotypic and genetic typing methods. | a combination of typing methods was used to identify the strains and the infection source in a food-borne outbreak of salmonella enterica occurring in a summer camp and affecting 25 children. all isolates tested were found to be serovar enteritidis, with an identical biotype api 20e and ribotype. however, they differed in their plasmid profiles and/or antibiograms, and were grouped into three strains. one strain was found in human stools, another in a hen's egg, and the third in both stools and ... | 1995 | 8549719 |
| salmonella enteritidis agfbac operon encoding thin, aggregative fimbriae. | salmonella enteritidis produces thin, aggregative fimbriae, named sef17, which are composed of polymerized agfa fimbrin proteins. dna sequence analysis of a 2-kb region of s. enteritidis dna revealed three contiguous genes, agfbac. the 453-bp agfa gene encodes the agfa fimbrin, which was predicted to be 74% identical and 86% similar in primary sequence to the escherichia coli curli structural protein, csga. phag, a puc18 derivative containing a 3.0-kb hindiii fragment encoding agfbac, directed t ... | 1996 | 8550497 |
| large outbreak of salmonella enterica serotype paratyphi b infection caused by a goats' milk cheese, france, 1993: a case finding and epidemiological study. | to assess the magnitude of a nationwide outbreak of infection with salmonella enterica serotype paratyphi b and identify the vehicle and source of infection. | 1996 | 8555937 |
| the salmonella dublin virulence plasmid does not modulate early t-cell responses in mice. | the virulence plasmid in salmonella dublin mediates systemic infection in mice and cattle. the role of gamma delta t cells or hepatic extrathymic t cells has recently been reported to be important in the control of the early stage of salmonella choleraesuis infections of mice. here, we report on t-cell responses in conventional mice after challenge with a virulent strain of s. dublin carrying a virulence plasmid or with a strain cured of the plasmid. over a period of 4 days postinfection, when b ... | 1996 | 8557343 |
| dna fingerprint analysis of standard, intermediate, and variant antigenic types of salmonella enterica subspecies enterica serovar gallinarum biovar pullorum. | dna fingerprint profiles and antigenic types of 150 salmonella enterica serovar gallinarum biovar pullorum field isolates and seven reference strains were evaluated. field isolates were submitted to the national veterinary services laboratories (nvsl), ames, iowa, between oct. 1, 1990, and aug. 20, 1991. three antigenic types (standard [n = 96], intermediate [n = 50], and variant [n = 4]) were identified among the field isolates. the ncii fingerprint profiles of all field isolates were identical ... | 1995 | 8561745 |
| natural transmission of salmonella choleraesuis in swine. | this experiment was designed to study the natural transmission of salmonella choleraesuis in swine. forty pigs were divided into three groups. group 1 (n = 12) was challenged with 10(8) cfu of s. choleraesuis per ml by intranasal inoculation. one day postinoculation (p.i.), group 2 (n = 24) was commingled with group 1. group 3 (n = 4) served as uninoculated controls. serum samples were collected weekly. blastogenesis assays and necropsies were performed at 1, 2, 4, 6, 9, and 12 weeks p.i., and 1 ... | 1996 | 8572691 |
| salmonella enterotoxin (stn) gene is prevalent among strains of salmonella enterica, but not among salmonella bongori and other enterobacteriaceae. | all strains and serovars of salmonella enterica such as serovar typhimurium, enteritidis, dublin, typhi, etc. were found to carry the salmonella enterotoxin determinant stn as far as examined in pcr and hybridization studies. however, using mdck cells for testing the toxicity of the strains under investigation, only a limited number of stn positive strains revealed phenotypically the salmonella enterotoxin stn. in contrast to s. enterica, other enterobacteriaceae including salmonella bongori wer ... | 1995 | 8580901 |
| gene transfer is a major factor in bacterial evolution. | lateral gene transfer in four strains of salmonella enterica has been assessed using genomic subtraction. strain lt2 (subspecies i serovar typhimurium) chromosomal dna was used as target and subtracted by three subspecies i strains of serovars typhimurium (s21), muenchen (s71), typhi (m229), and a subspecies v strain (m321). data from probing random cosmids of lt2 dna with preparations of the residual lt2 dna after subtraction were used to estimate the amounts of lt2 dna not able to hybridize to ... | 1996 | 8583905 |
| polymorphism of rrna genes and plasmid analysis in the typing of salmonella enterica serovar enteritidis from a spanish health area. | a molecular epidemiological study of salmonella enterica serovar enteritidis (s. enteritidis) in a spanish health area was carried out using two genotypic methods; polymorphism of rrna genes (ribotyping) and plasmid analysis. the series included 100 isolates randomly selected from among those collected over the period 1984-92 (50 from sporadic episodes and 50 from 10 outbreaks). ribotyping using hindiii, smai, and ecori showed a notable degree of homogeneity within enteritidis serovar, it being ... | 1995 | 8590390 |
| influence of inoculation route on the carrier state of salmonella choleraesuis in swine. | this study was designed to investigate the carrier state of swine infected with salmonella choleraesuis. thirty-five pigs were divided into 3 groups. groups 1 (n = 15) and 2 (n = 16) were challenged with 10(8) cfu of s. choleraesuis intranasally or by gastric route, respectively. group 3 (n = 4) served as uninoculated controls. pigs were necropsied at 2, 4, 6, and 12 weeks post inoculation. clinical signs and microscopic lesions were more severe for group 1. salmonella choleraesuis was recovered ... | 1995 | 8604554 |
| methanol extracts lps from deep rough bacteria. | several rough strains of escherichia coli, salmonella enterica and proteus mirabilis were cultivated in the presence of (14c)acetate, which incorporated into their phospholipids and lipopolysaccharide. phospholipids were removed from the cells with ethanol extraction. however, as shown by thin layer chromatography, methanol additionally extracted remarkable quantities of lipopolysaccharide from deep rough strains, but not from bacteria containing complete or nearly complete core. | 1996 | 8605006 |
| [epidemiological studies in 2 episodes of food poisoning by salmonella enterica serovar enteritidis and the phenotypic and genotypic characterization of the epidemic strains]. | 1994 | 8611233 | |
| specific detection of salmonella enterica serotype enteritidis using the polymerase chain reaction. | an assay was developed for the specific detection of salmonella enterica serotype enteritidis, using a novel application of the polymerase chain reaction (pcr). this pcr assay is based on the mismatch amplification mutation assay, an allele-specific reaction, and can discriminate enteritidis from all other salmonella. pcr primers were selected to amplify a 351-base pair (bp) dna fragment from the salmonella plasmid virulence a (spv a) gene of enteritidis. a single base difference at position 272 ... | 1996 | 8620904 |
| c-terminal half of salmonella enterica wbap (rfbp) is the galactosyl-1-phosphate transferase domain catalyzing the first step of o-antigen synthesis. | we previously showed that the product of the wbap gene of salmonella enterica serovar typhimurium has two functions: it is involved in the first step of o-antigen synthesis (the galactosyltransferase [gt] function) and in a later step (the t function), first thought to be the flipping of the o-antigen subunit on undecaprenyl pyrophosphate from the cytoplasmic face to the periplasmic face of the cytoplasmic membrane. we now locate two wbap(t) mutations within the first half of the wbap gene by se ... | 1996 | 8626328 |
| linkage of genes essential for synthesis of a polysaccharide capsule in sphingomonas strain s88. | several structurally related capsular polysaccharides that are secreted by members of the genus sphingomonas are being developed as aqueous rheological control agents for diverse industrial and food applications. they include gellan (s-60), welan (s-130), rhamsan (s-194), s-657, s-88, s-198, s-7, and nw-11. we refer to these polysaccharides as sphingans, after the genus name. this paper characterizes the first gene cluster isolated from a sphingomonas species (s88) that is required for capsule s ... | 1996 | 8626338 |
| virulence and genotype stability of salmonella enterica serovar berta during a natural outbreak. | strains of salmonella enterica serotype berta, collected over a period of 6 years from a well documented natural outbreak in denmark, have been characterized in order to assess the stability of chromosomal typing systems and virulence properties. outbreak strains were identical in pvu ii and psti is200 profiles, all but two strains showed the same sma i ribotype, and all but one strain showed the same not i pulsed field gel electrophoretic pattern, indicating that these molecular markers remaine ... | 1996 | 8666069 |
| growth hormone modulates il-alpha and ifn-gamma release by murine splenocytes activated by lps or porins of salmonella typhimurium. | the effect of growth hormone (gh) on the release of il-1alpha and ifn-gamma from murine splenocytes was investigated. their release from splenocytes activated by salmonella enterica serovar typhimurium lipopolysaccharide (lps) 0.5 microg/ml was increased by c. 65% in the presence of gh 100 pg/ml. with splenocytes activated by s. typhimurium porins 5 microg/ml, gh increased the production of both il-1alpha and ifn-gamma by c. 56%. polymyxin treatment abolished the cytokine-releasing activity of l ... | 1996 | 8667411 |
| effect of dose on the immune response and persistence of salmonella choleraesuis infection in swine. | to establish the effect of dose on persistence of and immune response to salmonella choleraesuis in swine. | 1996 | 8669761 |
| [foodborne diseases: a survey on working methods used by 6 public health services in the lombardy region. 1. outbreaks]. | since foodborne diseases, especially those caused by bacteria, have become an increasingly important public health problem, the authors conducted a survey in order to evaluate the organization of, and the intervention carried out by, six public health services in the lombardia region, after reports of foodborne diseases outbreaks. lack of correct methodology was detected, not to mention the usual omission of epidemic curves and attack rates. besides, too many microbiological tests were made and ... | 1995 | 8679179 |
| a family of lysozyme-like virulence factors in bacterial pathogens of plants and animals. | we describe a conserved family of bacterial gene products that includes the virb1 virulence factor encoded by tumor-inducing plasmids of agrobacterium spp., proteins involved in conjugative dna transfer of broad-host-range bacterial plasmids, and gene products that may be involved in invasion by shigella spp. and salmonella enterica. sequence analysis and structural modeling show that the proteins in this group are related to chicken egg white lysozyme and are likely to adopt a lysozyme-like str ... | 1996 | 8692991 |
| cytokine release by porcine livers perfused with lipopolysaccharide or live salmonella choleraesuis. | to develop a model to study the kinetics and relative amounts of cytokines produced by liver cells during enteric infection. | 1996 | 8712509 |
| [detection of resistance to the amoxicillin-clavulamic acid combination in salmonella and shigella]. | to study the emergence of amoxicilline/clavulanate resistance in salmonella and shigella. | 1996 | 8714185 |
| prevalence and serovar distribution of salmonella in fresh and frozen meat from captive nile crocodiles (crocodylus niloticus). | salmonella was isolated from 6 of 20 (30.0%) samples of fresh meat, and from 28 of 140 (20.0%) samples of frozen meat processed for human consumption from captive nile crocodiles (crocodylus niloticus) in zimbabwe. salmonella enterica isolates showed a serovar distribution of 41.2% (14/34) subsp. enterica, 11.8% (4/34) subsp. salamae and 41.2% (14/34) subsp. diarizonae. analyses of fresh meat samples yielded aeromonas (a. hydrophila) in 18 of 20 samples (90%), and a mean aerobic plate count (apc ... | 1996 | 8722192 |
| molecular analysis of salmonella enterica serotype dublin: building bridges between population genetic and molecular epidemiological studies. | population genetic studies of salmonella enterica serotype dublin using multilocus enzyme electrophoresis have recognised two dominant clones termed du1 and du3. the characterisation of plasmids in dublin suggests greater strain diversity. the application of restriction enzyme fragmentation pattern (refp) analysis of genomic dna using sau3a and hincii together with plasmid subtractive analysis can resolve anomalies in earlier comparisons. twenty-six isolates were selected for inclusion in the st ... | 1996 | 8738324 |
| the salmonella virulence plasmid enhances salmonella-induced lysis of macrophages and influences inflammatory responses. | the salmonella dublin virulence plasmid mediates systemic infection in mice and cattle. here, we analyze the interaction between wild-type and plasmid-cured salmonella strains with phagocytes in vitro and in vivo. the intracellular recovery of s. dublin from murine peritoneal and bovine alveolar macrophages cultured in the presence of gentamicin in vitro was not related to virulence plasmid carriage. however, the virulence plasmid increased the lytic activity of s. dublin, salmonella typhimurium ... | 1996 | 8757880 |
| distribution, gene sequence and expression in vivo of the plasmid encoded fimbrial antigen of salmonella serotype enteritidis. | the pefa gene which encoded the serotype associated plasmid (sap) mediated fimbrial major subunit antigen of salmonella enterica serotype typhimurium shared genetic identity with 128 of 706 salmonella isolates as demonstrated by dot (colony) hybridization. seventy-seven of 113 isolates of typhimurium and individual isolates of serotypes bovis-morbificans, cholerae-suis and enteritidis phage type 9b hybridized pefa strongly, whereas 48 isolates of enteritidis hybridized pefa weakly and one enteri ... | 1996 | 8760946 |
| world health organisation--supervised interlaboratory comparison of elisas for the serological detection of salmonella enterica serotype enteritidis in chickens. | a collaborative exercise, supervised by the world health organisation, was set up to compare elisas used for the serological detection of salmonella enteritica serotype enteritidis in chickens. the aim was to ascertain how far agreement could be reached on the interpretation of optical density readings for high titre, intermediate titre and low titre sera. two sets of sera were sent to 14 participants. the first set compared high, medium and low titre sera raised in specified-pathogen-free and c ... | 1996 | 8760952 |
| isolates of salmonella enterica enteritidis pt4 with enhanced heat and acid tolerance are more virulent in mice and more invasive in chickens. | two enteritidis pt4 isolates which differed in inherent tolerance to heat, acid, h2o2 and the ability to survive on surfaces were used to infect mice, day-old chicks or laying hens. the acid-, heat-, h2o2- and surface-tolerant isolate was more virulent in mice and more invasive in laying hens, particularly in reproductive tissue. however, no significant differences were observed in behaviour in chicks. both pt4 isolates were able to infect chicks housed in the same room as infected birds, althou ... | 1996 | 8760953 |
| epidemiology of salmonella enterica serotype enteritidis infections in southern italy during the years 1980-1994. | increased frequency of identification of salmonella enterica serotype enteritidis as a causative agent of sporadic and epidemic cases of infection in humans, along with isolation in many parts of the world of strains belonging in a large proportion to a few phage types, has made phage typing alone inadequate for epidemiological investigations. in southern italy the epidemic increase in isolation of s. enterica serotype enteritidis that has been observed since 1990 has been associated in approxim ... | 1996 | 8763625 |
| [salmonella freetown: 1st isolation in argentina]. | in this paper we report the first case of salmonella freetown in argentina. it was isolated from a stool sample of a child of 1 month and 7 days, assisted as outpatient in the hospital de niños dr. ricardo gutiérrez of the city of santa fe. on the basis of the biochemical characteristics and antigenic formulae, this new serovar belongs to the species salmonella enterica subspecies enterica. | 1996 | 8768486 |
| typing of salmonella enterica serovar saintpaul: an outbreak investigation. | during the summer of 1993 an outbreak of human salmonellosis caused by salmonella serovar saintpaul occurred in denmark. a total of 35 isolates originating from pigs, turkeys and imported foodstuffs, and 10 human isolates were compared following their characterization by agglutination of the 0:5 factor, antibiogram typing, plasmid profiling, ribotyping and pulsed field gel electrophoresis, in order to identify the most probable source of infection. after typing, the source of the investigated ou ... | 1996 | 8774669 |
| antibacterial activity of a synthetic peptide (pr-26) derived from pr-39, a proline-arginine-rich neutrophil antimicrobial peptide. | pr-39 is a proline-arginine-rich (pr) neutrophil antibacterial peptide originally identified and purified from the porcine small intestine. we report on the synthesis of a functional antibacterial domain of pr-39, the first 26 amino acid residues of the nh2 terminus. pr-26 was as potent as or more potent than pr-39 against enteric gram-negative bacteria. this truncated form of pr-39 potentiated neutrophil phagocytosis of salmonella choleraesuis and decreased the level of s. typhimurium invasion ... | 1996 | 8787891 |
| the identification, cloning and mutagenesis of a genetic locus required for lipopolysaccharide biosynthesis in bordetella pertussis. | bordetella pertussis lipopolysaccharide (lps) is biologically active, being both toxic and immunogenic. using transposon mutagenesis we have identified a genetic locus required for the biosynthesis of lps in b. pertussis, which has been cloned and sequenced. we have also identified equivalent loci in bordetella bronchiseptica and bordetella parapertussis and cloned part of it from b. parapertussis. the amino acid sequences derived from most of the genes present in the sequenced b. pertussis locu ... | 1996 | 8821935 |
| biology and clinical significance of virulence plasmids in salmonella serovars. | non-typhoid salmonella strains containing virulence plasmids are highly associated with bacteria and disseminated infection in humans. these plasmids are found in salmonella serovars adapted to domestic animals, such as salmonella dublin and salmonella choleraesuis, as well as in the widely distributed pathogens salmonella typhimurium and salmonella enteritidis. although virulence plasmids differ between serovars, all contain a highly conserved 8-kb region containing the spv locus that encodes t ... | 1995 | 8845442 |
| partial purification and characterization of salmonella cytotoxin. | the cytotoxins of two strains of salmonella enterica subspecies enterica serovar nchanga (mp2309) and virchow (mp1871) were partially purified and characterized physicochemically. the toxin was salt-precipitable and non-dialysable. upon filtration through sephadex g-100 gel column, the toxicity was observed mainly in 2nd peak. the cytotoxic activity was lost completely when heated at 121 degrees c for 15 min. the toxin was found active between a narrow range of ph (i.e., 6.0 to 9.0). the cytotox ... | 1996 | 8861639 |
| phage types and ribotypes of salmonella enteritidis in southern italy. | differently from other european countries, southern italy was affected by a considerable increase in human infections due to salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) only after 1990. on the present investigation, two groups of s. enteritidis strains isolated during the low-incidence period 1980-1984 and the epidemic period 1990-1993, respectively, have been submitted to phage-typing and ribotyping in order to ascertain whether the epidemic increase was determined ... | 1996 | 8861878 |
| molecular typing of yersinia pseudotuberculosis by using an is200-like element. | the is200-like insertion sequence (is) is a 708-bp element recently found in yersinia pestis. its nucleotide sequence is 85% identical to that of is200 recovered in most salmonella enterica isolates. it is also present in multiple copies in y. pseudotuberculosis. in contrast, this is is found in some (biotype 1b strains) but not other y. enterocolitica strains and is absent in the nonpathogenic yersiniae: y. frederiksenii, y. kristensenii, y. intermedia, y. bercovieri, and y. mollaretii. the num ... | 1996 | 8862590 |
| a novel relationship between o-antigen variation, matrix formation, and invasiveness of salmonella enteritidis. | salmonella enterica enteritidis in chickens serves as a reservoir for salmonellosis in humans and the structure of its lipopolysaccharide (lps) has been used to assess invasiveness. culture from chick spleens generated colonies with an unusual wrinkled morphology, and it is designated the lacy phenotype. the characterize the nature of the morphological change, three isogenic variants were compared. only the lacy phenotype produced a temperature-dependent cell surface matrix composed of several p ... | 1996 | 8870619 |
| investigation of an outbreak of human salmonellosis caused by salmonella enterica ssp. enterica serovar infantis by use of pulsed field gel electrophoresis. | analysis of chromosomal dna restriction patterns produced by pulsed field gel electrophoresis (pfge) was used to investigate an outbreak of human salmonellosis caused by salmonella enterica ssp. enterica serovar infantis (s. infantis) involving more than 500 registered human cases. the outbreak had been tentatively traced back to a single pig slaughterhouse. a total of 135 isolates from various sources produced 21 different pfge patterns with the restriction endonuclease xbai. all human isolates ... | 1996 | 8880333 |
| molecular characterization of salmonella enterica subsp. enterica serovar choleraesuis field isolates and differentiation from homologous live vaccine strains suisaloral and sc-54. | four independent molecular methods were used to characterize the salmonella enterica subsp. enterica serovar choleraesuis live vaccine strains sc-54 and suisaloral and to differentiate them from s. choleraesuis field isolates. plasmid analysis revealed the presence of seven plasmid profiles. a virulence plasmid of 52-kbp was identified by hybridization with an spvb-spvc gene probe in each of the s. choleraesuis field isolates and in the suisaloral vaccine strain, but not in the sc-54 vaccine str ... | 1996 | 8880500 |
| idiopathic disseminated bacillus calmette-guérin infection: a french national retrospective study. | disseminated bacillus calmette-guérin (bcg) infection after inoculation of live vaccine is considered to result from impaired immunity of the child. however, in half of the cases, regarded as idiopathic, no well-defined immunodeficiency condition can account for the infection. the objective of the present study is to report the prevalence, clinical features, associated infections, and outcomes of children with idiopathic disseminated bcg infection. | 1996 | 8885960 |
| high mutation frequencies among escherichia coli and salmonella pathogens. | here it is reported that the incidence of mutators among isolates of pathogenic escherichia coli and salmonella enterica is high (over 1 percent). these findings counter the theory, founded on studies with laboratory-attenuated strains, that suggests mutators are rare among bacterial populations. defects in methyl-directed mismatch repair underlie all mutator phenotypes described here. of nine independently derived hypermutable strains, seven contained a defective muts allele. because these muta ... | 1996 | 8895473 |
| characterization of a nosocomial outbreak involving an epidemic plasmid encoding for tem-27 in salmonella enterica subspecies enterica serotype othmarschen. | a ceftazidime-resistant salmonella enterica subspecies enterica serotype othmarschen strain, harboring the plasmid-mediated new extended-spectrum beta-lactamase tem-27, was involved in a nosocomial outbreak (8 patients) at the pediatric cardiology department of the ramón y cajal hospital in madrid. genomic dna polymorphism analysis, using an arbitrarily primed polymerase chain reaction, demonstrated the clonal nature of all salmonella isolates. the plasmid encoding tem-27 (pjmm1) was characteriz ... | 1996 | 8896503 |
| molecular typing of salmonella enterica serovar typhi. | the efficiencies of different tests for epidemiological markers--phage typing, ribotyping, is200 typing, and pulsed-field gel electrophoresis (pfge)--were evaluated for strains from sporadic cases of typhoid fever and a well-defined outbreak. ribotyping and pfge proved to be the most discriminating. both detected two different patterns among outbreak-associated strains. | 1996 | 8897193 |