Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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[induction of il2 responsiveness in allergen-stimulated lymphocytes from children with post respiratory syncytial virus (rsv) infection]. | induced interleukin 2 (il2) responsiveness in allergen-stimulated lymphocytes from children with post respiratory syncytial virus (rsv) infection was studied. induction of il2 responsiveness in the patients lymphocytes was observed upon stimulation with allergens such as dermatophgoides farinae (df) antigen, ovalbumin (ova) and alpha-casein. such responses were not induced in age-matched normal lymphocytes. maximum response was shown in lymphocytes from children who had been suffering from the i ... | 1992 | 1622335 |
rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool. | we compared the detection of seven respiratory viruses by using a commercially available monoclonal antibody pool in a 2-day shell vial assay with that by using standard cell culture with respiratory syncytial virus (rsv) enzyme-linked immunosorbent assay (elisa)-negative nasal secretions from hospitalized children. we found 179 respiratory virus isolates by either method in 675 specimens. overall, the shell vial assay detected 147 of 179 (79%) of the positives after 2 days; cell culture detecte ... | 1992 | 1624569 |
sequence analysis of the 22k, sh and g genes of turkey rhinotracheitis virus and their intergenic regions reveals a gene order different from that of other pneumoviruses. | the nucleotide and deduced amino acid sequences of three genes of turkey rhinotracheitis virus (trtv) together with the nucleotide sequences of the relevant intergenic regions were determined. the deduced amino acid sequence of one of the genes shows significant identity (42%) to that of the 22k protein of human respiratory syncytial virus (rsv). the trtv 22k gene, like that of rsv, has a second open reading frame, although the amino acid sequence deduced from this reading frame does not show an ... | 1992 | 1629697 |
oligomerization and post-translational processing of glycoprotein g of human respiratory syncytial virus: altered o-glycosylation in the presence of brefeldin a. | the post-translational maturation of the attachment g glycoprotein of human respiratory syncytial virus (rsv) was investigated. the g protein formed homo-oligomers which sedimented in sucrose gradients at the same rate as the fusion f protein tetramer. oligomerization of the g protein was insensitive to carbonylcyanide m-chlorophenylhydrazine, showing that this step occurs in the endoplasmic reticulum prior to o-glycosylation which initiated in the trans-golgi compartment. the sedimentation of t ... | 1992 | 1634876 |
[evaluation of 5 methods for the diagnosis of infections by respiratory syncytial virus]. | to compare and evaluate four different rapid methods of respiratory syncytial virus antigen detection with the isolation of the virus in cell culture. | 1992 | 1643129 |
complete nucleotide sequences of two soybean mosaic virus strains differentiated by response of soybean containing the rsv resistance gene. | the complete nucleotide sequence of the genomic rnas of strains g2 and g7 of soybean mosaic virus were determined. in both cases, the genome is 9588 nucleotides long, excluding the 3'-terminal poly(a) sequence. a large open reading frame (nucleotides 132 to 9329) encodes a polyprotein of 3066 amino acids with a predicted m(r) of either 349542 (strain g2) or 349741 (strain g7). based on comparison with the proposed locations of cleavage sites of other potyvirus polyproteins, nine mature proteins ... | 1992 | 1645142 |
transcription of muscle-specific genes is repressed by reactivation of pp60v-src in postmitotic quail myotubes. | quail myogenic cells infected with temperature sensitive (ts) mutants of rous sarcoma virus (rsv) exhibit a temperature-dependent transformation and block of differentiation. when the cells are allowed to differentiate at the restrictive temperature (41 degrees c) and then shifted back to the permissive temperature (35 degrees c), a sharp reduction in the accumulation of muscle-specific mrnas is observed, following reactivation of the transforming protein pp60v-src. a kinetic analysis of this do ... | 1991 | 1645448 |
dimerization of human immunodeficiency virus (type 1) rna: stimulation by cations and possible mechanism. | the retroviral genome consists of two identical rna molecules joined close to their 5' ends by the dimer linkage structure. recent findings indicated that retroviral rna dimerization and encapsidation are probably related events during virion assembly. we studied the cation-induced dimerization of hiv-1 rna and results indicate that all in vitro generated hiv-1 rnas containing a 100 nucleotide domain downstream from the 5' splice site are able to dimerize. rna dimerization depends on the concent ... | 1991 | 1645868 |
rapid diagnosis of viral respiratory infections. comparison between immunofluorescence on clinical samples and immunofluorescence on centrifuged cell cultures. | we compared the technique of immunofluorescence on clinical samples (ifcs) and immunofluorescence on centrifuged cell cultures (ifcc) for the detection of respiratory viruses, using monoclonal antibodies. four hundred fourty five nasopharyngeal aspirates were tested prospectively for rsv, influenza- (a and b), parainfluenza viruses (1, 2, 3) and adenovirus. ifcs and ifcc detected 94% and 51% of rsv respectively, 78.5% and 82% of influenza viruses, 8.3% and 100% of parainfluenzaviruses and 0 and ... | 1991 | 1645920 |
production of a transforming growth factor-beta-like growth factor by rsv-transformed rat cerebral microvascular endothelial cells. | this study describes the preliminary characterization of a rous sarcoma virus (rsv)-transformed rat cerebral microvascular endothelial cell (rce-t1)-derived growth factor with biological properties similar to transforming growth factor-beta (tgf-beta). a significant portion of this rce-t1-derived tgf-beta-like growth factor was secreted in an active form and its secretion was elevated 2- to 3-fold in late passage cultures comprised of rapidly growing, less differentiated cells exhibiting reduced ... | 1991 | 1648783 |
[virological surveillance of acute respiratory tract illnesses of children in morioka, japan. i. epidemiological patterns of infection with respiratory viruses over a 10-year period]. | a virological surveillance of acute respiratory tract illnesses (rti) of children in morioka, japan, was maintained from september 1973 to december 1983. nasal and throat swabs were collected from 4,334 children with rti. these patients consisted of 3,500 children (80.8%) with upper rti (urti) and 834 children (16.2%) with lower rti. when these patients with urti were classified by maximum temperature recorded into 4 groups (less than or equal to 37.0 degrees c, 37.1-37.9 degrees c, 38.0-38.4 de ... | 1991 | 1649233 |
pathogenesis of severe acute respiratory infections in the developing world: respiratory syncytial virus and parainfluenza viruses. | respiratory syncytial virus (rsv) and parainfluenza viruses (pivs) are the most frequently isolated pathogens in infants and children with acute lower respiratory infection (lri) in the developed world. less information is available about their importance in lri in the developing world, but they are probably important there also. the pathogenesis of viral bronchiolitis and pneumonia involves inoculation and early replication in the upper respiratory tract, followed by aspiration into the lower r ... | 1991 | 1650494 |
modulation of intercellular adherens-type junctions and tyrosine phosphorylation of their components in rsv-transformed cultured chick lens cells. | transformation of cultured chick lens epithelial cells with a temperature-sensitive mutant of rous sarcoma virus (tsrsv) leads to radical changes in cell shape and interactions. when cultured at the restrictive temperature (42 degrees c), the transformed cells largely retained epithelial morphology and intercellular adherens junctions (aj), whereas on switch to the permissive temperature (37 degrees c) they rapidly became fibroblastoid, their aj deteriorated, and cell adhesion molecules (a-cam) ... | 1991 | 1650581 |
v-src genes in two cell lines of so-called rsv-transformed human cells are defective and inactive. | trial of rous sarcoma virus (rsv) induction by cell fusion with chick embryo cells (cec) and wing web test from so-called rsv-transformed human cells, kc and rsb cells, was unsuccessful. the loss of rsv inducibility was also confirmed by dna transfection method. southern blot and northern blot hybridization of dna and rna from those cells with the v-src probe revealed that the v-src genes in those cells were defective and not expressed. on the other hand, the v-src gene in rsv-transformed mouse ... | 1991 | 1651045 |
[induction of unstable mutations in drosophila melanogaster by microinjections of oncogenic virus dna into the embryo polar plasma. prolonged genetic instability of mutations at the lobe locus]. | mutations lobe induced by the microinjections of rsv cdna into drosophila melanogaster early embryos are characterized by permanent genetic instability; the level of this instability is being changed in time. based on the results of genetic analyses of lobe mutations and molecular analysis of white and adh mutations induced at high frequency in this system of gene instability, we supposed that unstable mutations which arose under the influence of retroviral cdna are of the insertional nature. | 1991 | 1652537 |
invasion by wc5 rat cerebellar cells is independent of rsv-induced changes in growth and adhesion. | the wc5 rat cerebellar cell line, which is infected with a rous sarcoma virus that is temperature-sensitive for pp60src transformation, shows temperature-dependent expression of the neural-cell-adhesion molecule (n-cam) and glial fibrillary acidic protein (gfap). we found that wc5 cells maintained at the non-permissive temperature in both monolayer cultures and spheroids are subject to density-dependent inhibition of growth, whereas cells maintained at the permissive temperature continued to gro ... | 1991 | 1652569 |
transformation-dependent activation of urokinase-type plasminogen activator by a plasmin-independent mechanism: involvement of cell surface membranes. | transformed cells produce elevated levels of the urokinase-type plasminogen activator (u-pa), which has been linked with the invasive or migratory phenotype of these cells. the u-pa is secreted and normally maintained in the inactive, single-chain form (scu-pa) and it has been assumed that natural activation occurs via a plasmin-mediated cleavage converting scu-pa to the active, two-chain form (tcu-pa). we now demonstrate that secreted scu-pa in rous sarcoma virus-transformed chicken embryo fibr ... | 1991 | 1652763 |
isolation of adenovirus from lambs with upper respiratory syndrome. | the role of viruses in the etiology of recurrent upper respiratory disease in newly weaned lambs was studied during 1984-1985 at the north dakota sheep experiment station. serum samples collected from lambs at weaning, from lambs with signs of respiratory disease, and 3 weeks following the onset of clinical signs were tested for antibodies to ovine adenovirus (oav), respiratory syncytial virus (rsv), and parainfluenza type-3 virus (pi-3). virus isolation studies were performed on nasal secretion ... | 1991 | 1655060 |
assembly and processing of avian retroviral gag polyproteins containing linked protease dimers. | assembly and maturation of retroviral particles requires the aggregation and controlled proteolytic cleavage of polyprotein core precursors by a precursor-encoded protease (pr). active, mature retroviral pr is a dimer, and the accumulation of precursors at sites of assembly may facilitate subunit interaction and subsequent activation of this enzyme. in addition, it has been suggested that cellular cytoplasmic components act as inhibitors of pr activity, so that processing is delayed until the na ... | 1991 | 1656090 |
complex expression pattern of the cef-4 cytokine in transformed and mitogenically stimulated cells. | the transformation of chicken embryo fibroblasts (cef) by the rous sarcoma virus leads to the constitutive expression of the cellular gene designated cef-4. with specific antisera, we confirmed that transformed cells actively synthesize and secrete a 6 kda polypeptide corresponding to the cef-4 gene product. the expression of cef-4 was investigated by northern and immunoprecipitation analyses. upon activation of pp60v-src in cells infected by a ts mutant of rsv, the expression of cef-4 was bipha ... | 1991 | 1656364 |
characterization of rous sarcoma virus intronic sequences that negatively regulate splicing. | retroviruses splice only a fraction of their primary rna transcripts to subgenomic mrna. the unspliced rna is transported to the cytoplasm, where it serves as genomic rna as well as mrna for the gag and pol genes. deletion of sequences from the rous sarcoma virus gag gene, which is part of the intron of the subgenomic mrnas, was previously observed to result in an increase in the ratio of spliced to unspliced rna. these sequences, which we termed a negative regulator of splicing (nrs), can be mo ... | 1991 | 1656608 |
pexpress: a family of expression vectors containing a single transcription unit active in prokaryotes, eukaryotes and in vitro. | we have constructed a family of expression vectors containing a single transcription unit that is active in escherichia coli, eukaryotic cells, and in coupled in vitro transcription-translation systems. these vectors use the rous sarcoma virus-long terminal repeat (rsv-ltr) as the promoter/enhancer for eukaryotic cells. in vitro transcription is made possible by inclusion of a bacteriophage t7 promoter. this same promoter is actively transcribed in e. coli that produce t7 rna polymerase. other f ... | 1991 | 1657716 |
the isopycnic, compartmentalized integration of rous sarcoma virus sequences. | rous sarcoma virus (rsv) can cause tumors in hamsters, which harbor complete or partially deleted rsv sequences, in their genomes. here we have studied the localization of rsv sequences integrated into the genome of cell lines derived from six independent hamster tumors. we have found that integration occurred in the isochores richest in guanine + cytosine, of the host genome, as it had been previously observed for bovine leukemia and hepatitis b viral sequences. the integration of rsv proviral ... | 1991 | 1657723 |
importance of sv40 early 5' distal sequences in directing heterologous gene expression. | the chloramphenicol acetyltransferase-encoding reporter gene (cat) is used extensively in assessing the ability of transcriptional regulatory elements (tre) to direct gene expression in eukaryotic cells. two commonly utilized plasmids contain the cat coding sequences under the transcriptional control of the rous sarcoma virus ltr (prsvcat) or simian virus 40 early (sv40e) promoter (psv2cat). in the present study, we have recloned the rsv-ltr and sv40e tre into a puc18 vector. direct comparison o ... | 1991 | 1657724 |
viral pneumonia. | viral pneumonias are common in infants and young children but rare in adults. respiratory syncytial virus (rsv) and para-influenza viruses are the most frequent viral pathogens in infants and children. influenza virus types a and b account for over one half of viral pneumonias in adults. immunocompromised hosts are susceptible to pneumonias caused by cytomegalovirus (cmv) and other herpesviruses, as well as rubeola and adenovirus. diagnosis of viral pneumonia depends on appropriate viral culture ... | 1991 | 1659594 |
elevation of nerve growth factor synthesis by constitutive expression of v-src oncogene in cultured rat fibroblasts. | rat fibroblast 3y1 cells transformed by rous sarcoma virus (rsv) or transfected with the v-src gene showed a highly constitutive v-src gene expression. simultaneously, marked increases in the cellular level of nerve growth factor (ngf) mrna and ngf content in the culture medium were observed. the levels of ngf mrna and ngf secreted into the medium were correlated with the expression level of v-src mrna gene in both transformants and control 3y1 cells. these results suggest that v-src gene expres ... | 1991 | 1660581 |
detection of rotavirus antigen in tracheal aspirates of infants and children with pneumonia. | clinical manifestations of respiratory tract infection often precede or coincide with rotavirus gastroenteritis in infants and children. to investigate the possible association between respiratory tract manifestations and rotavirus infection, the authors determined human rotavirus (hrv) antigen and respiratory syncytial virus (rsv) antigen in tracheal aspirates of 58 children with clinically diagnosed pneumonia by enzyme-linked immunosorbent assay (elisa) and immunofluorescent antibody technique ... | 1991 | 1661224 |
a new variant of rous sarcoma virus-33 nondefective, pathogenic for rats. | rous sarcoma virus-33 (rsv-33) belongs to rsvs that have the least number of passages beyond its isolation from chicken tumor no. 1 among all current strains of rsv. biological characterization indicated that it was pathogenic for rats. the results of the proviral restriction enzyme analysis showed that the established rat tumorigenic cell lines were the most likely infected by the same virus having a full-length genome. | 1991 | 1662779 |
[virological surveillance of acute respiratory tract illnesses of children in morioka, japan. ii. rhinovirus infection]. | rhinoviruses (hrvs) were isolated from 307 children (7.1%) in the virological surveillance of 4334 children with acute respiratory tract illnesses in morioka, japan (september 1973-december 1983). although hrvs were isolated throughout the year, frequency of hrv infection was significantly higher (p less than 0.001) during the april-november (233/2853; 8.2%) than during the december-march (47/1481; 5.0%). there were two peaks of incidence in may (9.5%) and september (9.1%). during the may-septem ... | 1991 | 1665166 |
synthesis and structure-activity relationships of 2',3'-dideoxypurine nucleosides as potential antiretroviral agents. | in order to study the structure-activity relationships of 2',3'-dideoxypurine nucleosides as potential anti-retroviral agents, various purine derivatives have been synthesized and tested against rous sarcoma virus (rsv) in chick embryo fibroblast (cef) cells, and against human immunodeficiency virus (hiv) in human cd4+ t cells (ath8). some of the new purine derivatives found to be significant antiretroviral activities. and the correlations of activity between anti-rsv and anti-hiv have shown fai ... | 1991 | 1668777 |
longitudinal study of acute respiratory diseases in rio de janeiro: occurrence of respiratory viruses during four consecutive years. | the occurrence of different viruses in nasopharyngeal secretions from children less than 5 years old with acute respiratory infections (ari) was investigated over a period of 4 years (1982-1985) in rio de janeiro. of the viruses known to be associated with ari, all but influenza c and parainfluenza types 1, 2 and 4 were found. viruses were found more frequently in children attending emergency or pediatric wards than in outpatients. this was clearly related to the high incidence of respiratory sy ... | 1991 | 1668974 |
suppressed expression of icam-1 and lfa-1 and abrogation of leukocyte collaboration after exposure of human mononuclear leukocytes to respiratory syncytial virus in vitro. comparison with exposure to influenza virus. | human mononuclear leukocytes (mnl) exposed to respiratory syncytial virus (rsv) produce net il-1 inhibitor bioactivity with the anticipated consequences of cell cycle arrest, suppressed virus-specific proliferation, and reduced expression of activation markers. these studies were undertaken to investigate effects of exposure and resultant net il-1 inhibitor activity on the expression of the intercellular adhesion molecule-1 (icam-1), and its ligand the lymphocyte function-associated antigen (lfa ... | 1991 | 1677945 |
modulation of retinal differentiation by oncogenes: effect of the v-src gene on expression of choline acetyltransferase and glutamine synthetase. | expression of the protooncogene c-src in chick neural retina is developmentally regulated and associated with neural differentiation. in the present study, chick neural retina (nr) cell cultures from 7 day embryos were exposed to the exogenous src oncogene, the c-src counterpart, to establish the effect of expression of v-src on specific retinal cellular differentiation. nr cells from 7 day chick embryos were placed in monolayer or rotation culture and infected with rous sarcoma virus (rsv) cont ... | 1991 | 1681112 |
isolation of a catalytically competent phosphorylated tyrosine kinase from rous sarcoma virus-induced rat tumor by immunoadsorption to and hapten elution from phosphotyrosine binding antibodies. | a procedure has been developed for the isolation of a catalytically competent phosphorylated tyrosine kinase (rsv y-kinase) from avian sarcoma virus-induced rat tumors. the procedure involves reaction of partially purified rsv y-kinase with atp to effect tyrosyl phosphorylation of catalytically competent rsv y-kinase. tyrosyl phosphorylated rsv y-kinase was isolated from the heterogeneous reaction mixture by immunoadsorption on immobilized phosphotyrosyl binding antibodies and elution with the h ... | 1990 | 1690027 |
phosphorylation of connexin43 gap junction protein in uninfected and rous sarcoma virus-transformed mammalian fibroblasts. | gap junctions are membrane channels that permit the interchange of ions and other low-molecular-weight molecules between adjacent cells. rous sarcoma virus (rsv)-induced transformation is marked by an early and profound disruption of gap-junctional communication, suggesting that these membrane structures may serve as sites of pp60v-src action. we have begun an investigation of this possibility by identifying and characterizing putative proteins involved in junctional communication in fibroblasts ... | 1990 | 1690850 |
demonstration of respiratory syncytial virus in an autopsy series. | respiratory syncytial virus (rsv) antigen was demonstrated in formalin-fixed, paraffin-embedded autopsy tissue using an immunoperoxidase technique. eighteen autopsy cases were selected on the basis of one of the following criteria: a positive culture for rsv, antemortem or postmortem; positive elisa test for rsv, antemortem or postmortem; or postmortem histology suggestive of paramyxovirus infection. controls included three cases from which parainfluenza or influenza virus had been cultured and ... | 1990 | 1695371 |
[recombinant rna-dependent dna-polymerase from rous sarcoma virus. isolation and properties]. | preparations of recombinant reverse transcriptase rsv were isolated from e. coli hb101/pmf14 cell cultures. the enzyme purified to homogeneity was shown to be made up of two subunits with molecular masses of 97 +/- 4 and 61 +/- 3 kda. a comparison of enzymatic properties of recombinant transcriptase to those of the enzyme isolated from the rsv (rauss sarcoma) virus demonstrated that in the preparations under study the recombinant reverse transcriptase exists in a subunit form, alpha beta, and ma ... | 1990 | 1696135 |
establishment and characterization of sv40 t-antigen immortalized human esophageal epithelial cells. | normal human esophageal autopsy tissue was explanted in serum-free medium. the epithelial outgrowths were subcultured and then transfected by strontium phosphate coprecipitation with plasmid prsv-t consisting of the rsv-ltr promoter and the sequence encoding the simian virus 40 large t-antigen. the transfected cells, but not the sham-transfected controls, formed multilayered colonies within 3-4 weeks, after which the colonies were transferred and cell strains (he-451 and he-457) developed. both ... | 1991 | 1703038 |
construction of a recombinant adenovirus containing the denv gene from bacteriophage t4 which can partially restore the dna repair deficiency in xeroderma pigmentosum fibroblasts. | the denv gene from bacteriophage t4 encodes a pyrimidine dimer-specific endonuclease that has the capacity to initiate excision repair of dna. cells from excision repair-deficient xeroderma pigmentosum (xp) patients are able to carry out excision repair initiated by the denv gene product and introduction of the denv gene into xp cells results in the partial restoration of colony-forming ability after irradiation with uv light. in this work we have constructed a helper-independent recombinant hum ... | 1991 | 1704821 |
induction of morphological change by tyrosine kinase inhibitors in rous sarcoma virus-transformed rat kidney cells. | erbstatin and methyl 2,5-dihydroxycinnamate, related tyrosine kinase inhibitors, induced a morphological change in temperature-sensitive rous sarcoma virus-transformed rat kidney (rsvts-nrk) that brought the cells close to the morphology of their normal counterpart. erbstatin did not change the morphology of normal or kirsten sarcoma virus-transformed rat kidney cells. erbstatin also inhibited morphological transformation of rsvts-nrk cells induced by a shifting in temperature. actin stress fibr ... | 1991 | 1704855 |
cell/substratum adhesions in rsv-transformed rat fibroblasts. | cell/substratum adhesions have been studied in rat fibroblasts transformed by a ts-mutant of rous sarcoma virus (la-29) using light and electron microscopy and a variety of preparative methods including immunolabeling. cells were studied both during the process of transformation, i.e., shifting from 39 degrees to 35 degrees c, and in a fully transformed state (passaged at 35 degrees c continuously). the typical focal contacts observed at 39 degrees c (restrictive temperature) were replaced by "p ... | 1991 | 1706278 |
synthetic peptides corresponding to the f protein of rsv stimulate murine b and t cells but fail to confer protection. | we have previously located a major neutralization site of the fusion protein of respiratory syncytial virus (rsv) in the polypeptide region extending from amino acids ile221 to glu232. in this report, 8 peptides corresponding to the six major hydrophilic regions of the f1 subunit were selected to analyse their immunogenic and protective capacities as well as their ability to block the high neutralization activities of 4 monoclonal antibodies (mabs). only 5 of the 8 peptides tested induced specif ... | 1991 | 1706591 |
immunodominant t-cell epitope on the f protein of respiratory syncytial virus recognized by human lymphocytes. | the lymphocyte proliferative responses to respiratory syncytial virus (rsv) were evaluated for 10 healthy adult donors and compared with proliferative responses to a chimeric glycoprotein (fg glycoprotein) which consists of the extracellular domains of both the f and g proteins of rsv and which is produced from a recombinant baculovirus. the lymphocytes of all 10 donors responded to rsv, and the proliferative responses to the whole virus were highly correlated with the responses to the fg glycop ... | 1991 | 1710289 |
analysis of cdnas of the proto-oncogene c-src: heterogeneity in 5' exons and possible mechanism for the genesis of the 3' end of v-src. | to further characterize the gene structure of the proto-oncogene c-src and the mechanism for the genesis of the v-src sequence in rous sarcoma virus, we have analyzed genomic and cdna copies of the chicken c-src gene. from a cdna library of chicken embryo fibroblasts, we isolated and sequenced several overlapping cdna clones covering the full length of the 4-kb c-src mrna. the cdna sequence contains a 1.84-kb sequence downstream from the 1.6-kb pp60c-src coding region. an open reading frame of 2 ... | 1991 | 1712905 |
antiretroviral activities of anthraquinones and their inhibitory effects on reverse transcriptase. | alizarin complexone (ac), alizarin red s (ars) and various other anthraquinones were evaluated for their inhibitory effects on rous-associated virus 2 reverse transcriptase (rav-2 rt). some 1,2-dihydroxyanthraquinones were active against this enzyme and ac was the most potent inhibitor among these compounds [50% inhibitory concentration (ic50): 3.8 micrograms/ml]. ac slightly inhibited rous sarcoma virus rt (rsv rt) and human immunodeficiency virus type 1 rt (hiv-1 rt) (ic50: 100 micrograms/ml a ... | 1991 | 1716088 |
recombinant reverse transcriptase of rous sarcoma virus: characterization of dna polymerase and rnaase h activities. | enzyme preparations of rous sarcoma virus (rsv) reverse transcriptase have been isolated from a culture of e. coli hb101(pmf14). the enzyme has been purified to homogeneity and been shown to consist of two subunits, of molecular mass 97.4 and 61.3 kda, respectively. the optimum conditions for the dna polymerase and rnaase h activities, fidelity of dna synthesis on a homogeneous rna template, and the inhibitory effect of azidothymidine triphosphate have been determined. data on the use of rsv rec ... | 1991 | 1717011 |
use of intravenous gamma globulin to passively immunize high-risk children against respiratory syncytial virus: safety and pharmacokinetics. the rsvig study group. | infants with cardiopulmonary disease develop severe illness from respiratory syncytial virus (rsv) infection. safety, feasibility, and pharmacokinetics of intravenous gamma globulin (ivig) to prevent rsv illness were studied in 23 high-risk infants in a phase i trial. ivig with an rsv neutralizing antibody titer of 1:1,100 in 5% solution was given monthly over a 2- to 4-h period in a clinical setting during the rsv season. the first group (n = 7) received 500 mg/kg of body weight, the second gro ... | 1991 | 1718213 |
differential inhibitory effects of sulfated polysaccharides and polymers on the replication of various myxoviruses and retroviruses, depending on the composition of the target amino acid sequences of the viral envelope glycoproteins. | sulfated polysaccharides (i.e., dextran sulfate) and sulfated polymers (i.e., sulfated polyvinylalcohol and sulfated copolymers of acrylic acid with vinylalcohol) were found to be potent and selective inhibitors of the replication of respiratory syncytial virus (rsv) and influenza virus type a (influenza a virus) but not of other myxoviruses (parainfluenza 3, measles, and influenza b viruses). the compounds were also inhibitory to human immunodeficiency virus type 1 (hiv-1) and hiv-2 and simian ... | 1991 | 1725692 |
respiratory syncytial virus infections in pediatric liver transplant recipients. | respiratory syncytial virus (rsv) is the most important cause of lower respiratory tract infection in infants and young children. the charts of 17 children found to have rsv among 493 children who underwent liver transplantation between february 1985 and february 1991 were reviewed. the median age at diagnosis was 20 months. median time of diagnosis was 24 days after transplantation. thirteen patients developed nosocomial infections while convalescing from their transplant. common symptoms inclu ... | 1992 | 1727886 |
eosinophil degranulation in the respiratory tract during naturally acquired respiratory syncytial virus infection. | eosinophil cationic protein (ecp), a cytotoxic protein contained in the granules of eosinophils, has been suggested as having an important role in the pathogenesis of asthma. to determine whether ecp plays a similar role in bronchiolitis, we tested samples of nasopharyngeal secretions, obtained from a group of 47 children with various forms of illness related to respiratory syncytial virus (rsv) and from 26 children with non-rsv upper respiratory tract illness or bacterial pneumonia, for the pre ... | 1992 | 1731020 |
resistance to respiratory syncytial virus (rsv) challenge induced by infection with a vaccinia virus recombinant expressing the rsv m2 protein (vac-m2) is mediated by cd8+ t cells, while that induced by vac-f or vac-g recombinants is mediated by antibodies. | it was previously demonstrated that the vaccinia virus recombinants expressing the respiratory syncytial virus (rsv) f, g, or m2 (also designated as 22k) protein (vac-f, vac-g, or vac-m2, respectively) induced almost complete resistance to rsv challenge in balb/c mice. in the present study, we sought to identify the humoral and/or cellular mediators of this resistance. mice were immunized by infection with a single recombinant vaccinia virus and were subsequently given a monoclonal antibody dire ... | 1992 | 1731105 |
further studies with short duration ribavirin aerosol for the treatment of influenza virus infection in mice and respiratory syncytial virus infection in cotton rats. | ribavirin aerosol administration has been shown to be effective in the treatment of respiratory syncytial virus (rsv) infections in infants and in influenza a and b virus infections in young adults. long treatment schedules and potential for environmental contamination have stimulated the search for alternative dosing schedules. thus, we attempted to determine the length of time of ribavirin aerosol necessary for effective treatment of influenza and rsv. in rsv-infected cotton rats, aerosolizati ... | 1992 | 1736809 |
viral respiratory infections in the institutionalized elderly: clinical and epidemiologic findings. | to prospectively evaluate the incidence and impact of viral respiratory infection in the institutionalized elderly during a winter season. | 1992 | 1740594 |
respiratory syncytial virus (rsv): a common health problem. | rsv infections pose a variety of considerations for emergency personnel. nurses should be suspicious of this cause of illness during the winter months because of its prevalence, should be able to recognize and treat those who are experiencing or are at risk for acquiring lower respiratory tract complications, and should be aware of the need to reduce the spread of the virus by using isolation techniques, vigorous hand-washing protocol, and other protective measures to reduce contact with the org ... | 1992 | 1740879 |
cytokine (tumor necrosis factor, il-6, and il-8) production by respiratory syncytial virus-infected human alveolar macrophages. | human alveolar macrophages (am) are susceptible to infection with respiratory syncytial virus (rsv), but the infection is abortive after the initial cycles of virus replication. we have investigated if rsv infection of am results in the production of cytokines tnf, il-6, and il-8, all of which may modulate inflammatory and immune responses to the virus, as well as may directly protect respiratory epithelial cells against spread of infection. within 1 h after interaction with rsv, increased mrna ... | 1991 | 1753101 |
[detection of rs viral sequences in otitis media effusion from children]. | the role of respiratory syncytial virus (rs virus) in otitis media with effusion was studied. reverse transcriptase-polymerase chain reaction (rt-pcr) and nested polymerase chain reaction (nested pcr) were used to provide accurate methods for detection of rs viral sequences in the otitis media effusion from children. we could find rs viral sequences in 10/12 samples tested which had been collected during and even after natural outbreak of rsv in the community. these observations suggest rs virus ... | 1991 | 1757848 |
occurrence of respiratory syncytial virus subtypes in hospitalized children in cleveland, ohio from 1985 to 1988. | in order to determine the frequency of occurrence of the two respiratory syncytial virus (rsv) subtypes in hospitalized children in cleveland, ohio, we analyzed clinical isolates obtained during three consecutive winter epidemic seasons between 1985 and 1988. rsv was recovered from the frozen clinical specimens of 197 patients: 176 subtype a, and 21 subtype b. subtype a predominated during all three epidemic seasons, ranging from 83 to 94% of isolates. we surveyed the clinical records of 16 chil ... | 1991 | 1758737 |
the cold war against rsv. | respiratory syncytial virus (rsv) can cause substantial morbidity and mortality in high risk patients. the authors discuss prevention and treatment of this virus. | 1991 | 1765449 |
immune responses of lambs experimentally infected with bovine respiratory syncytial virus and pasteurella haemolytica. | the immune responses of lambs experimentally infected with bovine respiratory syncytial virus (rsv) and pasteurella haemolytica were compared with lambs infected with bovine rsv or pasteurella haemolytica alone. superinfection with p. haemolytica aggravated the reduction in the number of cd5+, cd4+ and lca p220+ (b) lymphocytes caused by bovine rsv, but it had no effect on the neutralizing antibodies against p. haemolytica cytotoxin. serum samples obtained from lambs experimentally infected with ... | 1991 | 1779037 |
evaluation of a commercial enzyme immunomembrane filter assay for detection of respiratory syncytial virus in clinical specimens. | a commercial enzyme immunomembrane filter assay (eifa) for respiratory syncytial virus (rsv) was compared prospectively with isolation in cell culture and an enzyme immunoassay. a total of 595 respiratory specimens, mostly from pediatric patients, were examined. the eifa was 70.96% sensitive and 72.40% specific in comparison with cell culture. results for 40 specimens (6.72%) were uninterpretable, mainly due to filtration difficulties. twenty-one (25%) of 84 specimens whose results were initiall ... | 1991 | 1802701 |
failure to detect paramyxovirus sequences in paget's disease of bone using the polymerase chain reaction. | it is widely believed that paget's disease of bone is due to a "slow virus" infection of osteoclasts with one of the paramyxovirus group. controversy continues to surround the identity of the virus involved, however, since at different times evidence has been presented implicating measles virus (mv), respiratory syncytial virus (rsv), and canine distemper virus (cdv) as putative infective agents. in this study we used the technique of reverse transcription and polymerase chain reaction (pcr) to ... | 1991 | 1805546 |
comparative study of respiratory syncytial virus in nasopharyngeal aspirates using conventional cell culture, shell viral centrifugation culture, immunofluorescence and biotin-avidin enzyme linked immunosorbent assays. | 133 nasopharyngeal aspirates (npa) were simultaneously tested for the presence of respiratory syncytial virus (rsv) by conventional cell culture (ccc), shell vial centrifugation culture (svc), immunofluorescence assay (ifa) and biotin-avidin enzyme linked immunosorbent assay (b-a elisa). these yielded positive results in 32(24%), 45(33.8%), 36(27%) and 40(30%) of specimens, respectively. specimens positive by ifa and b-a elisa were all also positive by svc. the sensitivity of ccc, ifa, and b-a e ... | 1991 | 1807259 |
expression of a human monoclonal anti-hiv-1 antibody in cho cells. | the cdna coding for the light and heavy chains, respectively, of the human monoclonal antibody 3d6 (igg1, kappa), which binds specifically to human immunodeficiency virus-1 (hiv-1) gp41, was inserted into three different mammalian expression vectors and transfected into chinese hamster ovary (cho) cells. transcription was under the control of rous sarcoma virus long terminal repeat (rsv ltr), human cytomegalovirus major immediate early (cmv ie) promoter, and mouse mammary tumor virus long termin ... | 1991 | 1809191 |
a chimeric glycoprotein of human respiratory syncytial virus termed fg induces t-cell mediated immunity in mice. | popliteal lymph node cells taken from mice vaccinated with the fg glycoprotein were exposed in vitro to respiratory syncytial virus (rsv) antigens. proliferation to fg or rsv antigens was blocked with anti-cd4 monoclonal antibody treatment. fg-vaccinated mice developed classical late delayed type hypersensitivity (dth) reactions when exposed to fg antigen in vivo. | 1991 | 1811371 |
severe bronchiolitis in children. | acute viral bronchiolitis is one of the most common causes of paediatric respiratory disease, especially in the first year of life. young infants and those with pre-existent cardiorespiratory problems are at an increased risk of acute respiratory failure necessitating mechanical ventilation. a prospective study was carried out from november 1988 to october 1989, to analyse the clinical features, aetiology and outcome of patients with severe bronchiolitis. 52 (29.9%) out of 174 cases of acute bro ... | 1991 | 1812334 |
clinical characteristics of respiratory syncytial virus (rsv) subgroup infections in japan. | the subgroup characteristics of 130 strains of respiratory syncytial virus (rsv) isolated in sapporo during 9 epidemic years 1980-1989 were determined. monoclonal antibodies raised against the rsv long strains were used. subgroup a included 77 (59.2%) isolates and subgroup b 52 (40.0%) strains, while 1 strain was considered to be a variant of a subgroup a strain. the distribution by age of infants and children was different for the 2 subgroups: less than 1 year of age infants with subgroup a inf ... | 1991 | 1815326 |
nucleotide sequences of the 3' leader and 5' trailer regions of human respiratory syncytial virus genomic rna. | the nucleotide sequences of the 3' extracistronic (leader) and 5' extracistronic (trailer) regions were determined for genomic rna (vrna) of human respiratory syncytial virus (rsv) strain a2. to sequence the 3' leader region, vrna was extracted from purified virions, size-selected, polyadenylated, copied into cdna, amplified by the polymerase chain reaction, cloned, and sequenced. the 3' leader sequence is 44 nt, which is somewhat shorter than its counterparts (50 to 70 nt) in other nonsegmented ... | 1991 | 1840712 |
isolation of a population of transiently transfected quiescent and senescent cells by magnetic affinity cell sorting. | rous sarcoma virus (rsv) and cytomegalovirus (cmv) promoters were tested for activity in proliferating and nonproliferating (quiescent or senescent) human embryo fibroblasts. these promoters were cloned upstream of the coding sequence for the tac subunit of the interleukin 2 receptor, and activity was calculated from the fraction of tac antigen positive cells detected in a coupled transient transfection/magnetic affinity cell sorting assay. differences in promoter activities are substantial in q ... | 1991 | 1845795 |
amino acids encoded downstream of gag are not required by rous sarcoma virus protease during gag-mediated assembly. | rous sarcoma virus (rsv) and its relatives are unique in that they appear to encode their viral protease in the gag reading frame. as a result, this 124-amino-acid sequence is found at the carboxy terminus of each gag precursor molecule and, upon ribosome frameshifting, embedded within each gag-pol molecule. however, rigorous proof has never been obtained for the activity of this 124-amino-acid gag domain during virion assembly in vivo. if the active protease actually included amino acids encode ... | 1991 | 1845888 |
drosophila alcohol dehydrogenase: a novel reporter gene for use in mammalian embryos. | a reporter gene construct containing the drosophila alcohol dehydrogenase (adh) gene under regulation of the rous sarcoma virus long terminal repeat (rsv ltr) was microinjected into mouse zygote pronuclei. adh activity after injection of the rsv-adh construct was visualized in cultured embryos by means of a simple histochemical enzyme assay. the rsv ltr was an efficient promoter that led to abundant adh activity at all stages of preimplantation development. there was a high incidence of mosaicis ... | 1991 | 1846008 |
inhibition of transient gene expression with plasmids encoding herpes simplex virus type 1 ul55 and alpha genes. | herpes simplex virus type 1 (hsv-1) subgenomic sequences from 0.743 to 0.782 map units have been molecularly cloned as plasmid at1 and shown to inhibit stable dna-mediated gene transformation of ltk- cells with the hsv-1 thymidine kinase (tk) gene. here it is shown that at1 also inhibits transient gene expression. expression from the chloramphenicol acetyltransferase (cat) gene under the control of either the hsv-1 tk gene or the rous sarcoma virus (rsv) promoter was inhibited when cotransfected ... | 1991 | 1846642 |
invasion of rous sarcoma virus-transformed retinal cells: role of cell motility. | transformation of retinal neuro-epithelial cells by rous sarcoma virus (rsv) leads to many alterations in cell phenotype, including changes in cell movement, cell-cell adhesion and protease secretion. to define and quantitate the alterations in cell movement, we analyzed video recordings of cultured cells using the computer-assisted dynamic morphology system (dms). control neuro-epithelial cells showed very low levels of translocation and membrane activity. after transformation, neuro-epithelial ... | 1991 | 1847356 |
phosphorylation of the avian retrovirus integration protein and proteolytic processing of its carboxyl terminus. | the integration protein (in) of the prague a strain of rous sarcoma virus (rsv) was analyzed by high-resolution sodium dodecyl sulfate-polyacrylamide gel electrophoresis. three polypeptides of similar proportions and molecular mass (32 kda) were immunoprecipitated by an antiserum directed against the first 10 amino acids of the amino terminus of in. however, the faster-migrating nonphosphorylated polypeptide was not immunoprecipitated by two different polyclonal antisera directed against the las ... | 1991 | 1847443 |
defining nucleic acid-binding properties of avian retrovirus integrase by deletion analysis. | integration of retroviral dna into the host genome requires the activity of retrovirus-encoded integration protein in. we expressed rous sarcoma virus (rsv) in, 286 amino acid residues in length, by using in vitro transcription, followed by in vitro translation in rabbit reticulocyte lysate. the nucleic acid-binding activity of in vitro-translated in was assessed by using dna-cellulose affinity chromatography and poly(u)-sepharose affinity chromatography and by sedimentation analysis in the pres ... | 1991 | 1847445 |
localization and induced expression of fusion genes in the rat lung. | liposome-mediated gene transfer is useful for dna transfection into cells in culture. we wondered whether this method could be used to introduce new dna into the intact lung. fusion genes containing either the rous sarcoma virus (rsv) promoter or the mouse mammary tumor virus (mmtv) promoter (which contains glucocorticoid response elements) were linked to the bacterial gene chloramphenicol acetyltransferase (cat), an enzyme not present in mammalian cells. plasmids containing the rsv-cat fusion g ... | 1991 | 1848084 |
a range of catalytic efficiencies with avian retroviral protease subunits genetically linked to form single polypeptide chains. | molecular modeling based on the crystal structure of the rous sarcoma virus (rsv) protease dimer has been used to link the two identical subunits of this enzyme into a functional, single polypeptide chain resembling the nonviral aspartic proteases. six different linkages were selected to test the importance of different interactions between the amino acids at the amino and carboxyl termini of the two subunits. these linkages were introduced into molecular clones of fused protease genes and the l ... | 1991 | 1848230 |
isolation and characterization of a 70-kda metalloprotease (gelatinase) that is elevated in rous sarcoma virus-transformed chicken embryo fibroblasts. | chicken embryo fibroblasts (cef) transformed by rous sarcoma virus (rsvcef) secrete a 70-kda metallo-gelatinase at elevated levels over that of normal cef. the 70-kda enzyme has been purified from rsvcef conditioned medium and represents 1-3% of the total protein in the rsvcef conditioned medium. a 22-kda protein, which appears to be the avian form of the tissue inhibitor of metalloproteases (timp), is co-isolated in association with the 70-kda enzyme and can be separated from the enzyme by gel ... | 1991 | 1848240 |
genetic analysis of the rous sarcoma virus subgroup d env gene: mammal tropism correlates with temperature sensitivity of gp85. | subgroup d avian sarcoma and leukosis viruses can penetrate a variety of mammalian cells in addition to cells from their natural host, chickens. sequences derived from the gp85-coding domain within the env gene of a mammal-tropic subgroup d virus (schmidt-ruppin d strain of rous sarcoma virus [sr-d rsv]) and a non-mammal-tropic subgroup b virus (rous-associated virus type 2) were recombined to map genetic determinants that allow penetration of mammalian cells. the following conclusions were base ... | 1991 | 1848317 |
phosphatidylinositol-3 kinase is activated in v-src, v-yes, and v-fps transformed chicken embryo fibroblasts. | pi-3 kinase activity has been shown to associate with p60v-src. we found that immunoprecipitates of p60v-src exhibit an activity that catalyzes the formation of pi-3-p, pi-3,4-p2 and pip3 from pi, pi-4-p, and pi-4,5-p2, respectively. transformation of chicken embryo fibroblasts (cef) by p60v-src of rous sarcoma virus (rsv) caused elevation of pi-3-p, pi-3,4-p2, and pip3, suggesting that the pi-3 kinase may be activated in these cells. similar elevations were seen in cells transformed with the v- ... | 1991 | 1849246 |
specificity studies on retroviral proteinase from myeloblastosis-associated virus. | the specificity of the p15 proteinase of myeloblastosis-associated virus (mav) was tested with nonviral high molecular weight substrates and with synthetic peptides. peptides with sequences spanning known cleavage sites in viral polyproteins of rous sarcoma virus (rsv) and avian leukemia viruses, as well as in bsa and hsa, were synthesized, and the rate of their cleavage by the mav proteinase was compared. synthetic peptides require for successful cleavage at least 4 residues at the n-terminal s ... | 1991 | 1849425 |
structural and evolutionary relationships between retroviral and eucaryotic aspartic proteinases. | three-dimensional crystal structures of the homologous retroviral proteinases from rous sarcoma virus (rsv pr) and from human immunodeficiency virus (hiv-1 pr) are to a large extent similar and bear close resemblance to the six known structures of the bilobal fungal and mammalian aspartic proteinases. systematic three-dimensional structural superpositions were carried out between the retroviral and the eucaryotic aspartic proteinases. both retroviral enzymes were found to be similarly related to ... | 1991 | 1851433 |
mapping the transcriptional transactivation function of simian virus 40 large t antigen. | t antigen is able to transactivate gene expression from the simian virus 40 (sv40) late promoter and from several other viral and cellular promoters. neither the mechanisms of transactivation by t antigen nor the regions of t antigen required for this activity have been determined. to address the latter point, we have measured the ability of a set of sv40 large t antigen mutants to stimulate gene expression in cv-1 monkey kidney cells from the sv40 late promoter and rous sarcoma virus (rsv) long ... | 1991 | 1851853 |
effects of recombinant human alpha a interferon in gnotobiotic calves challenged with respiratory syncytial virus. | the effects of recombinant human alpha a interferon were studied in gnotobiotic calves challenged with respiratory syncytial virus (rsv). gnotobiotic calves given doses of interferon by intramuscular injection over five days showed a marked, dose-related, rise in rectal temperature and depression of circulating leucocytes. differential counts showed decreases in both lymphocytes and neutrophils. no significant pathological differences were found between treated and untreated calves, nor could an ... | 1991 | 1852059 |
[the resistance of in vivo selected tumor cells of malignant phenotype to the cytolytic action of activated peritoneal macrophages in vitro]. | using radioisotope cytolytic 3h-thymidine release assay the sensitivity of low-malignant spontaneously in vitro transformed hamster embryo cells (sthe strain) and its in vivo selected malignant variants (sthe-lm-4, sthe-lm-8, sthe-75/18) as well as rous sarcoma virus (rsv) (schmidt-ruppin strain) tumorigenic transformants (het-sr-1, tu-sr) were tested to cytolysis by peritoneal syrian hamster macrophages (mp) activated in vitro with levan, lps, mdp and pma. it has been shown that the only parent ... | 1991 | 1854964 |
etiology of acute respiratory infections in children in tropical southern india. | nasopharyngeal secretions and throat-swab specimens from 809 children less than 6 years old with acute respiratory infection were examined by culture and indirect immunofluorescence for the presence of virus or viral antigen. blood was cultured for the presence of bacteria in selected cases of lower respiratory infection (lri); pleural fluid also was cultured in cases of empyema. viruses were detected in 163 (49%) of 331 children with lri. respiratory syncytial virus (rsv) was the commonest agen ... | 1991 | 1862277 |
antibody enhancement of respiratory syncytial virus stimulation of leukotriene production by a macrophagelike cell line. | the clinical and epidemiologic features of respiratory syncytial virus (rsv) infections suggest that rsv-specific antibody may sometimes contribute to the disease process. recently, it has been demonstrated that virus-specific antibody can enhance rsv infection of macrophagelike cells in vitro. we evaluated the possibility that antibody might also enhance rsv stimulation of the bronchoactive mediator of inflammation leukotriene c-4 (ltc4)in a macrophagelike cell line, u937. the addition of rsv l ... | 1991 | 1870211 |
reinfection of mice with respiratory syncytial virus. | a balb/c model of respiratory syncytial virus infection has been developed in which high-titered replication occurs in lung, immunological infiltrates in lung can be detected histologically, and illness can be consistently reproduced. the immunodeterminants of rsv reinfection in this system were investigated by rechallenging mice with rsv early (less than 2 months after primary infection) and late (16 to 21 months after primary infection) and correlating illness and titer of rsv isolated from lu ... | 1991 | 1885946 |
reliability of two new test kits for rapid diagnosis of respiratory syncytial virus infection. | two new rapid enzyme immunoassays (eias) for detecting respiratory syncytial virus (rsv), directigen (becton dickinson microbiology systems) and testpack (abbott diagnostics) were compared with virus isolation and direct immunofluorescence by using fresh specimens. the sensitivities of both eias were low (72 to 73%), but when initial specimens were used, testpack had a high sensitivity (92%) in contrast to that of directigen (76%). because of its high sensitivity and specificity, testpack can be ... | 1991 | 1890186 |
community- and hospital-acquired respiratory syncytial virus infections in chile. | nasopharyngeal aspirates were obtained on admission from 614 patients younger than 2 years of age who were hospitalized in a ward for acute respiratory infections from june 1988 through october, 1989, in santiago, chile. patients in two rooms were followed during the cold seasons by sampling aspirates every other day during the child's entire hospital stay. clinical features were recorded daily. indirect monoclonal immunofluorescent assay and isolation in hep-2 were used for respiratory syncytia ... | 1991 | 1891287 |
tissue distribution of mucosal antibody-producing cells specific for respiratory syncytial virus in severe combined immune deficiency (scid) mice engrafted with human tonsils. | groups of c.b-17 scid mice were reconstituted intraperitoneally with human tonsillar mononuclear cells (hu-tmc) from children seropositive for antibody to respiratory syncytial virus (rsv) and subsequently challenged intraperitoneally with inactivated rsv or sham-immunized. the synthesis and the distribution characteristics of human antibody to rsv in various murine tissues were studied using an enzyme-linked immunospot assay (elispot). no specific antibody was observed in sham-immunized animals ... | 1991 | 1893614 |
high-level expression of human c-jun gene causes cellular transformation of chicken embryo fibroblasts. | to analyze the transforming activity of c-jun, a rous sarcoma virus (rsv) variant that carries human c-jun instead of v-src (jh1) was constructed. after infection onto chicken embryo fibroblasts (cef), jh1 formed foci with a titer comparable to that of wild-type rsv, and the infected cells grew in soft agar, indicating that the human c-jun gene has transforming potential, like the v-jun gene. the expression of fra-2, one of the recently isolated fos-related antigens, but not fos was detected in ... | 1991 | 1900269 |
characteristics of fusion of respiratory syncytial virus with hep-2 cells as measured by r18 fluorescence dequenching assay. | the characteristics of fusion of respiratory syncytial virus (rsv) with hep-2 cells were studied by the r18 fluorescence dequenching assay of membrane fusion. a gradual increase in fluorescence intensity indicative of virion-cell fusion was observed when r18-labeled rsv was incubated with hep-2 cells. approximately 35% dequenching of the probe fluorescence was observed in 1 h at 37 degrees c. fusion showed a temperature dependence, with significant dequenching occurring above 18 degrees c. the d ... | 1991 | 1906550 |
effect of respiratory syncytial virus infection on mice with protein malnutrition. | respiratory syncytial virus (rsv) pulmonary infection was produced in balb/c mice fed protein-deficient diets in an effort to understand the severity of viral pneumonia in infants in developing countries. as in previously published experiments with sendai virus, animals on the deficient diet became clinically malnourished, and certain aspects of their cell-mediated immunity were altered. the course of rsv infection in protein-deprived mice was essentially identical to that in normally nourished ... | 1991 | 1906929 |
enhancer mediated suppression of epsilon heavy-chain gene expression in a murine ige-producing hybridoma. | in vitro co-culture of ige-secreting hybridoma cells (b53) with spleen cells harvested from mice with established b53 tumours results in a specific, t cell-dependent suppression of epsilon-chain expression in the b53 cells. the role of immunoglobulin enhancers in the suppression of ige synthesis in b53 cells was examined by transfecting b53 cells with cat expression vectors containing the immunoglobulin heavy- or kappa light-chain intron enhancers or a rous sarcoma virus (rsv) ltr. when epsilon- ... | 1991 | 1907351 |
respiratory syncytial virus infection in anti-mu-treated mice. | balb/c mice were depleted of b cells by anti-mu treatment to investigate the pathogenesis of respiratory syncytial virus (rsv) infection in the absence of antibody. termination of rsv replication after primary infection occurred with the same kinetics in anti-mu-treated mice as in phosphate-buffered saline (pbs)-treated controls. yet, when rechallenged, anti-mu-treated mice were more permissive to rsv replication than pbs-treated controls. anti-mu-treated mice also experienced greater illness th ... | 1991 | 1908028 |
role of t lymphocyte subsets in the pathogenesis of primary infection and rechallenge with respiratory syncytial virus in mice. | the role of cd4+ and cd8+ t lymphocytes in terminating respiratory syncytial virus (rsv) replication, causing disease, and protecting from reinfection was investigated using a balb/c mouse model in which cd4+ or cd8+ lymphocytes or both were depleted by injections of mab directed against the respective mouse lymphocyte determinants. kinetics of rsv replication, illness, and pathology were assessed after primary infection and rechallenge. both cd4+ and cd8+ lymphocyte subsets were involved in ter ... | 1991 | 1909350 |
clinical observations on lower respiratory tract infections with special reference to serum ige levels. | to examine if the occurrence of wheezing among children with lower respiratory tract infections is associated with atopic predisposition, serum levels of ige were determined for groups of children with or without wheezing, during infection with mycoplasma pneumoniae or respiratory syncytial virus (rsv). in m. pneumoniae infection, more than 60% of wheezy children over 2 years of age showed a high ige level (200 iu/ml or more), while ige levels were less than 200 iu/ml in 73% of non-wheezy childr ... | 1991 | 1923666 |
subacute effects of respiratory syncytial virus infection on lung function in lambs. | we examined the effects of ovine respiratory syncytial virus (rsv) infection on lung mechanics, lung histology, and airway reactivity in lambs. nine lambs were inoculated with ovine rsv and seven control lambs with normal saline or viral media. serum neutralization titers were obtained prior to and 3 weeks post-inoculation (pi). open lung biopsies were performed 1 and 3 weeks pi. lung mechanics including dynamic compliance (cdyn), resistance of the lung (rl), and functional residual capacity (fr ... | 1991 | 1923668 |
respiratory syncytial virus infection among intubated adults in a university medical intensive care unit. | respiratory syncytial virus is the major cause of lower respiratory tract infection in children. adults who are immunocompromised, aged, institutionalized, and/or have underlying medical diseases may be at risk for severe rsv infection. intubated adults in an micu were evaluated for evidence of rsv infection. respiratory secretions were analyzed by cell culture and rsv eia. serologic testing was obtained. respiratory secretions from micu personnel with acute respiratory symptoms and patients adm ... | 1991 | 1935297 |
epidemiological picture of respiratory viral infections in croatia. | this paper presents the epidemiological study of respiratory viral infections in croatia from 1 september 1986 till 31 august 1987. a total of 527 patients with acute respiratory diseases were examined. their nasopharyngeal secretion and/or throat swab were taken and the viruses were demonstrated by the method of direct viral diagnosis (isolation and rapid immunofluorescent detection). this 12-month study on acute respiratory infections in croatia in 1986/1987 shows that viruses were the agents ... | 1991 | 1950639 |