Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the tendency of lentiviral open reading frames to become a-rich: constraints imposed by viral genome organization and cellular trna availability. | human immunodeficiency virus type 1 (hiv-1) and other lentiviridae demonstrate a strong preference for the a-nucleotide, which can account for up to 40% of the viral rna genome. the biological mechanism responsible for this nucleotide bias is currently unknown. the increased a-content of these viral genomes corresponds to the typical use of synonymous codons by all members of the lentiviral family (hiv, siv, biv, fiv, caev, eiav, visna) and the human spuma retrovirus, but not by other retrovirus ... | 1995 | 7666442 |
| induction of accelerated feline immunodeficiency virus disease by acute-phase virus passage. | development of feline immunodeficiency virus (fiv) infection in cats as a small animal model for lentiviral immunodeficiency disease has been hampered by the prolonged and variable disease course following experimental infection. to address this issue, we generated high-titer, unselected fiv stocks by pooling plasma from cats acutely infected with a subgroup c fiv isolate designated cabcpadyooc (fiv-c-pgammer). subsequent infection with this virus pool resulted in rapidly progressive, fatal dise ... | 1995 | 7666517 |
| vaccine-induced virus-neutralizing antibodies and cytotoxic t cells do not protect macaques from experimental infection with simian immunodeficiency virus sivmac32h (j5). | to gain further insight into the ability of subunit vaccines to protect monkeys from experimental infection with simian immunodeficiency virus (siv), two groups of cynomolgus macaques were immunized with either recombinant sivmac32h-derived envelope glycoproteins (env) incorporated into immune-stimulating complexes (iscoms) (group a) or with these siv env iscoms in combination with p27gag iscoms and three nef lipopeptides (group b). four monkeys immunized with recombinant feline immunodeficiency ... | 1995 | 7666529 |
| prospective hematologic and clinicopathologic study of asymptomatic cats with naturally acquired feline immunodeficiency virus infection. | prospective studies were performed over a 28- to 77-month period (median, 66 months) on 5 cats with naturally acquired feline immunodeficiency virus (fiv) infection in an attempt to correlate hematologic and clinicopathologic changes with the emergence of clinical disease. on presentation, all cats were asymptomatic; free of opportunistic infections; and had normal complete blood counts, bone marrow morphologies, marrow progenitor frequencies, and progenitor in vitro growth characteristics. duri ... | 1995 | 7674214 |
| b epitopes and selection pressures in feline immunodeficiency virus envelope glycoproteins. | in order to map linear b epitopes in feline immunodeficiency virus (fiv) envelope glycoproteins (env), a random library of fiv env polypeptides fused to beta-galactosidase and expressed in escherichia coli was screened by using sera from experimentally fiv-infected cats. we mapped five antibody-binding domains in the surface envelope glycoprotein (su1 to su5) and four in the transmembrane envelope glycoprotein (tm1 to tm4). immunological analysis with 48 serum samples from naturally or experimen ... | 1993 | 7678301 |
| a monoclonal antibody which blocks infection with feline immunodeficiency virus identifies a possible non-cd4 receptor. | monoclonal antibody vpg15 detects a 24-kda cell surface protein on feline cells permissive for infection with feline immunodeficiency virus (fiv). the antibody blocks infection of fiv-susceptible cells, and expression of the vpg15 marker is decreased in fiv-infected cells in vitro. these results suggest that the antibody may recognize an fiv receptor distinct from cd4. | 1993 | 7679750 |
| detection of b epitopes on the p24 gag protein of feline immunodeficiency virus by monoclonal antibodies. | seven monoclonal antibodies were obtained after immunization of mice with purified sodium dodecyl sulfate (sds)-disrupted feline immunodeficiency virus (fiv). six antibodies specifically bound antigens in the cytoplasm of fiv-infected cells as determined by indirect immunofluorescence and reacted with fiv p24 gag gene product in immunoblots. one reacted positively with virus-infected cells, but failed to recognize fiv structural proteins by immunoblotting. using competition binding studies, the ... | 1993 | 7681301 |
| derivatives of 4-amino-3,6-disulfonato-1,8-naphthalimide inhibit reverse transcriptase and suppress human and feline immunodeficiency virus expression in cultured cells. | we have developed a series of 4-amino-3,6-disulfonato-1,8-naphthalimide (adsn) derivatives in an attempt to create nontoxic compounds effective against lentivirus infections. the adsn derivative lucifer yellow ch ([n-(hydra zinocarbonyl)amino]-4-amino-3,6-disulfonato-1,8-naphthalimid e) (lych) was chosen as a parent compound because of its low toxicity in vivo and in vitro and its tendency to accumulate in monocyte/macrophages, a major reservoir for lentiviruses in vivo. several adsn derivatives ... | 1993 | 7684385 |
| enhanced neutralization of feline immunodeficiency virus by complement viral lysis. | the ability of complement to inactivate feline immunodeficiency virus (fiv) was examined. treatment of virus with complement plus sub-neutralizing titers of antiserum resulted in a significant reduction in the virus titer compared with treatment of the virus with complement or antibody alone. one of the mechanisms by which cat complement inactivates fiv was shown to be by viral lysis as determined by a reverse transcriptase release assay. kinetic studies revealed that viral lysis is initiated so ... | 1993 | 7685130 |
| neutralizing antibodies in cats infected with feline immunodeficiency virus. | sera from cats experimentally infected with five isolates of feline immunodeficiency virus (fiv) from various geographical regions and from fiv enzyme-linked immunosorbent assay-seropositive field cats from four european countries neutralized the petaluma strain of fiv (fiv-p), originally isolated in california, at high titers. in addition, fiv-p and a european isolate proved equally susceptible to neutralization by all sera tested. coupled with observations by fevereiro et al. (m. fevereiro, c. ... | 1993 | 7686186 |
| in vivo lymphocyte tropism of feline immunodeficiency virus. | feline immunodeficiency virus (fiv) infection in the cat is similar to human immunodeficiency virus type 1 infection in causing a selective reduction in cd4+ cell numbers, leading to inversion of the cd4+/cd8+ ratio. to determine whether fiv, similar to human immunodeficiency virus type 1, has a tropism for cd4+ cells, we examined the in vitro and in vivo susceptibilities of feline lymphocyte subpopulations to fiv infection. infection of interleukin-2-dependent cd4+ or cd8+ lymphocyte cultures w ... | 1993 | 7688819 |
| feline immunodeficiency virus infection: plasma, but not peripheral blood mononuclear cell virus titer is influenced by zidovudine and cyclosporine. | the plasma and peripheral blood mononuclear cell (pbmc) titer of feline immunodeficiency virus (fiv) in experimentally infected cats was assessed following administration of either zidovudine or cyclosporine. treatments were begun 24 h post infection (p.i.) and continued for 4 weeks. zidovudine treatment did not prevent establishment of infection with fiv, but plasma virus titers were significantly lower than controls at 2 weeks p.i. this reduction of plasma virus titer by zidovudine was not mai ... | 1993 | 7688949 |
| establishment of a feline t-lymphoblastoid cell line susceptible to feline immunodeficiency viruses. | a feline t-lymphoblastoid cell line susceptible to feline immunodeficiency viruses (fiv) was established and designated as yu-1 cells. yu-1 cells continued to grow over one year with more than 100 successive passages in the presence of human recombinant interleukin-2. surface antigens of yu-1 cells were feline pan-t+, cd4+, and cd8-. susceptibility of yu-1 cells to fiv strains were higher than that of the primary culture of the feline peripheral blood mononuclear cells, indicating that this cell ... | 1993 | 7689139 |
| serological diagnosis of feline immunodeficiency virus infection based on synthetic peptides from env glycoproteins. | feline immunodeficiency virus (fiv) is a lentivirus which infects domestic cats, causing an acquired immunodeficiency syndrome (aids). the aim of the present work was the development of an immunoassay for the diagnosis of fiv infection, using synthetic peptides from fiv envelope (env) glycoproteins. four peptides (8 to 11 amino acids long) corresponding to group-specific epitopes of fiv env extracellular (su) or transmembrane (tm) glycoproteins were synthesized. they were evaluated by enzyme-lin ... | 1993 | 7689247 |
| the feline immunodeficiency virus orf-a gene facilitates efficient viral replication in established t-cell lines and peripheral blood lymphocytes. | frameshift mutants corresponding to all of the identified open reading frames of feline immunodeficiency virus, including the orf-a gene, which has an unknown function, were constructed in vitro. upon transfection into cells, no significant difference between the phenotypes of orf-a mutant clones and those of wild-type clones was demonstrated. although only orf-a mutant virus among all mutant viruses from transfected cells showed infectivity in established t-cell lines, the replication and propa ... | 1993 | 7690413 |
| feline immunodeficiency virus: an epidemiological review. | from a synthesis of 59 serosurveys, we estimate and discuss influence of several parameters on feline immunodeficiency virus (fiv) infection. the overall infection rate estimated from 85,529 tested cats is high, 11.04%, besides the virus seems to be rather endemic. parameters influencing fiv infection are sex, age and roaming habits. neutering, breed and group size effects seem to be rather indirect. prevalence differences between countries and potential cofactors are also discussed. results sho ... | 1994 | 7697467 |
| comparison of fibrosarcomas that developed at vaccination sites and at nonvaccination sites in cats: 239 cases (1991-1992). | questionnaires were sent to veterinarians who had submitted a fibrosarcoma from a cat to the surgical pathology services of the veterinary schools of the university of pennsylvania and tufts university between jan 1, 1991 and june 30, 1992. questionnaire items included signalment, felv and feline immunodeficiency virus status, site of sarcoma, vaccination site, vaccines used, treatment, biologic behavior of the tumor, and final outcome. data were analyzed, using student's t-test for continuous d ... | 1994 | 7698923 |
| induction of feline acquired immune deficiency syndrome by feline leukemia virus: alteration in response to hormones in the hypothalamic-pituitary-gonadal system. | male kittens who were infected with the feline leukemia virus (felv) were found at various times after exposure to contain a sequence of dysfunction in their hypothalamic-pituitary-gonadal (hpg) system. to understand whether the involved endocrine glands in this system were damaged by felv, the hypothalamus, pituitary, and testes were tested for hormonal responsiveness in vivo and in vitro. the infected cats were administered with luteinizing hormone-releasing hormone (lhrh) and human chorionic ... | 1995 | 7700890 |
| feline immunodeficiency virus: an interesting model for aids studies and an important cat pathogen. | the lentivirus feline immunodeficiency virus (fiv) is a widespread pathogen of the domestic cat that is mainly transmitted through bites, although other means of transmission are also possible. its prevalence ranges from 1 to 10% in different cat populations throughout the world, thus representing a large reservoir of naturally infected animals. fiv resembles the human immunodeficiency virus (hiv) in many respects. similarities include the structural features of the virion, the general organizat ... | 1995 | 7704896 |
| molecular analysis of tumours from feline immunodeficiency virus (fiv)-infected cats: an indirect role for fiv? | five tumours, which arose in cats naturally or experimentally infected with feline immunodeficiency virus (fiv), were examined with molecular probes to establish tumour cell lineage and to screen for integrated viral sequences. three of the tumours were classed as b-cell lymphomas on the basis of morphology, immunocytochemistry, rearrangement of immunoglobulin heavy chain genes and lack of rearrangement of t-cell receptor (tcr) beta-chain genes. two of these b-cell tumours arose in specific path ... | 1995 | 7705953 |
| feline immunodeficiency virus infection--a model for hiv and aids? | 1995 | 7707329 | |
| analysis of leucocytes and lymphocyte subsets for different clinical stages of naturally acquired feline immunodeficiency virus infection. | we report alterations in leucocytes numbers and lymphocyte subset percentages, determined by flow cytometry, for three observed and previously defined clinical stages (asymptomatic carrier (ac), aids-related complex (arc) and aids) of naturally occurring fiv infection. unstaged fiv-positive cats had significantly lower numbers of total leucocytes (wcc) and neutrophils, lower percentages of pant+ and cd4+, lower cd4:cd8 ratio and a higher percentage of b cells compared with unstaged fiv-negative ... | 1994 | 7725628 |
| vertical transmission of feline immunodeficiency virus. | we studied vertical transmission of feline immunodeficiency virus (fiv) to determine whether it might provide a model with which to study intervention strategies for mother-to-offspring transmission of human immunodeficiency virus (hiv). we found that pregnant cats acutely infected with fiv (fiv-csu-2771) transmitted the virus to their offspring via both prenatal and postnatal routes. in utero transmission led to several pathogenic consequences including arrested fetal development, abortion, sti ... | 1995 | 7734190 |
| nucleotide sequence of feline immunodeficiency virus: classification of japanese isolates into two subtypes which are distinct from non-japanese subtypes. | seven isolates of feline immunodeficiency virus (fiv), shizuoka, yokohama, sendai-1, sendai-2, fukuoka, aomori-1, and aomori-2, were isolated from fiv-seropositive domestic cats in japan, and their proviral dnas were amplified by pcr. the nucleotide sequences of their env and gag genes were determined and compared with those of previously described isolates: u.s. and european isolates and one japanese isolate, tm2. phylogenetic analyses of complete env gene sequences demonstrate that worldwide i ... | 1995 | 7745712 |
| enhancement of feline immunodeficiency virus infection after immunization with envelope glycoprotein subunit vaccines. | cats were immunized three times with different recombinant feline immunodeficiency virus (fiv) candidate vaccines. recombinant vaccinia virus (rvv)-expressed envelope glycoprotein with (vgr657) or without (vgr657 x 15) the cleavage site and an fiv envelope bacterial fusion protein (beta-galactosidase-env) were incorporated into immune-stimulating complexes or adjuvanted with quil a. although all immunized cats developed antibodies against the envelope protein, only the cats vaccinated with the r ... | 1995 | 7745719 |
| the c/ebp site in the feline immunodeficiency virus (fiv) long terminal repeat (ltr) is necessary for its efficient replication and is also involved in the inhibition of fiv ltr-directed gene expression by pseudorabies virus icp4. | we investigated effects of site-specific mutation of the putative c/ebp binding site in the feline immunodeficiency virus (fiv) long terminal repeat (ltr) on the basal promoter activity in crandell feline kidney (crfk) cells and on replication efficiency in crfk cells and a t-lymphoblastoid cell line, mya-1 cells. mutation of the c/ebp site reduced the basal promoter activity in crfk cells and prevented efficient fiv replication in both crfk and mya-1 cells. gel-mobility-shift assay using nuclea ... | 1995 | 7747422 |
| early suppression of viremia by zdv does not alter the spread of feline immunodeficiency virus infection in cats. | prophylactic zidovudine (zdv) therapy in feline immunodeficiency virus (fiv) inoculated cats was evaluated for 12 months postinfection (pi) and 11 months post drug treatment. plasma fiv antigenemia was prevented in six of six zdv-treated and none of six untreated cats during the initial phase of infection. the present study is a continuation of that earlier work. cd4 lymphocyte numbers from zdv-treated cats were higher than in the untreated cats. cd8 lymphocytes numbers were maintained within co ... | 1995 | 7749786 |
| cdna cloning and eukaryotic expression of feline cd9. | a monoclonal antibody (vpg15) has been described which can block infection with feline immunodeficiency virus (fiv) and which recognizes the feline homologue of cd9. in order to study the role of feline cd9 in infection with fiv we have molecularly cloned a cdna encoding feline cd9 by r.a.c.e (rapid amplification of cdna ends). the amino acid sequence of feline cd9 displays 95.1, 93.8 and 90.7% homology to human, murine and bovine cd9, respectively. although feline cd9 appears most homologous to ... | 1995 | 7753050 |
| protection of neonatal kittens against feline immunodeficiency virus infection with passive maternal antiviral antibodies. | maternal antibodies from either vaccinated or feline immunodeficiency virus (fiv)-infected female cats (queens) were evaluated for their ability to protect kittens against homologous fiv infection. | 1995 | 7755911 |
| seroepidemiological survey of feline retrovirus infections in cats in taiwan in 1993 and 1994. | in order to investigate the prevalence of infections with three feline retroviruses feline immunodeficiency virus (fiv), feline leukemia virus (felv) and feline syncytial virus (fsv) in taiwan, we collected a total of 75 blood samples from cats from veterinary hospitals, a breeding cattery and a homeless shelter in 1993 and 1994. we examined the presences of anti-fiv and fsv antibodies and felv-p27 antigen in these samples by the indirect immunofluorescence and/or enzyme-linked immunosorbent ass ... | 1995 | 7756412 |
| coccidioidomycosis in 48 cats: a retrospective study (1984-1993) | coccidioidomycosis was diagnosed in 48 cats. forty-one cases were identified within a period of 3 years. coccidioides immitis was revealed by cytological or histopathological examinations, or culture in 70% of cats. the remaining 30% of cases were diagnosed by appropriate clinical signs, radiographic lesions, and serological test results. the average age of affected cats was 6.2 years with a median age of 5.0 years. fifty-four percent (n = 26) were female and 46% (n = 22) were male. domestic sho ... | 1995 | 7760314 |
| prognostic factors associated with radiotherapy of squamous cell carcinoma of the nasal plane in cats. | ninety cats were irradiated for treatment of squamous cell carcinoma of the nasal plane. the 1- and 5-year progression-free survival rates were 60.1 +/- 5.5% and 10.3 +/- 6.2%, respectively. analysis of progression-free survival times revealed that clinical stage and tumor proliferative fraction (estimated by the use of a proliferating cell nuclear-antigen immunohistochemical method) had significant prognostic value. conversely, coat color, presence of multiple facial carcinomas, histologic grad ... | 1995 | 7768722 |
| feline immunodeficiency virus (fiv)-specific cytotoxic t lymphocytes from chronically infected cats are induced in vitro by retroviral vector-transduced feline t cells expressing the fiv capsid protein. | we have previously reported the presence of feline immunodeficiency virus (fiv)-specific, major histocompatibility complex (mhc)-restricted cytolytic t lymphocytes (ctl) in experimentally fiv-infected cats. however, the fine specificity of the ctl and the role of individual fiv proteins in inducing fiv-specific ctl responses remain unknown. in this study, we examined the in vitro induction and activity of fiv p24 capsid-specific ctl obtained from cats that had been experimentally infected with f ... | 1995 | 7778274 |
| thoughts on retroviruses. | 1995 | 7782231 | |
| feline immunodeficiency virus as a model for development of molecular approaches to intervention strategies against lentivirus infections. | 1995 | 7793326 | |
| (r)-9-(2-phosphonylmethoxypropyl)-2,6-diaminopurine is a potent inhibitor of feline immunodeficiency virus infection. | the antiviral efficacy of acyclic nucleoside phosphonates, including 9-(2-phosphonylmethoxyethyl)adenine (pmea) and (r)-9-(2-phosphonylmethoxypropyl)-2,6-diaminopurine [(r)-pmpdap] against feline immunodeficiency virus (fiv) infection was determined. (r)-pmpdap showed the highest selectivity index (> 2,000) in vitro. treatment of experimentally fiv-infected asymptomatic cats with pmea or (r)-pmpdap had no effect on the cd4+/cd8+ ratio. however, mean plasma viral rna concentrations decreased sign ... | 1995 | 7793884 |
| lentiviral infection, immune response peptides and sleep. | the aberrant sleep documented in subjects with human immunodeficiency virus (hiv) infection is uniquely important because of the contribution this poor quality sleep makes to the fatigue, disability, and eventual unemployment that befalls these patients. especially given this importance in clinical care, the research on the prominent sleep changes described in hiv infection remains modest in quantity. the chronic asymptomatic stage of hiv infection is associated with the most intriguing and sing ... | 1995 | 7795894 |
| early events in lymph nodes during infection with siv and fiv. | to elucidate the initial pathogenic events in lymphoid organs, the major reservoir of virus in hiv infection, follow-ups of viral load, pathological changes and target cells were performed in the rhesus macaque sivmac251 model and in the cat fiv model. lymph nodes (ln) obtained from animals sacrificed at early time points following experimental inoculation were analysed by in situ hybridization for virus load and by combined immunohistochemistry and in situ hybridization for virus cellular tropi ... | 1994 | 7800949 |
| contribution of the feline langerhans cell to the fiv model. | in order to identify an equivalent of langerhans cells in cat stratified epithelia, we used a panel of monoclonal antibodies known to be reactive with membrane antigens present on human langerhans cells. the labelling was carried out by immunoperoxidase staining, for examination by light microscope, and by immunogold labelling, for electron microscopy. out of 18 antibodies tested, only one, mhm23 antibody, specific against cd18 antigen, presented reactivity with dendritic epithelial cells on eit ... | 1994 | 7800952 |
| [vaccination of cats against infection with feline leukemia virus (felv): first recombinant vaccine and the effect of a pre-existing infection with feline immunodeficiency virus (fiv)]. | a new recombinant felv vaccine was evaluated in 30 specified pathogen-free cats 10 months of age cats. the vaccine consisted of the non-glycosylated felv envelope protein p45, aluminium hydroxide and a saponin adjuvant. the cats (n = 18) were vaccinated twice intramuscularly, 3 weeks apart. all animals were challenged intraperitoneally with felv subgroup a, 18 weeks later. while 94% of the vaccinated cats showed no viraemia or were only transiently viraemic, 80% of the non-vaccinated animals bec ... | 1994 | 7801087 |
| histopathological changes in lymph nodes of cats experimentally infected with the feline immunodeficiency virus (fiv). | twelve specific-pathogen-free (spf) kittens aged 8-12 weeks were serially infected in pairs every 6 weeks, by the intraperitoneal route, with the feline immunodeficiency virus (fiv). three additional spf kittens were kept as controls. the infected animals were killed 10 weeks after inoculation, during the primary phase of the fiv infection. generalized lymphadenopathy (gl) was observed in the first three pairs of cats. all lymph nodes examined from the 12 infected cats showed histological change ... | 1994 | 7806702 |
| sleep patterns are disturbed in cats infected with feline immunodeficiency virus. | human immunodeficiency virus (hiv)-related sleep disturbances have been reported early in aids. likewise, the feline immunodeficiency virus (fiv), a natural lentivirus pathogen of cats, produces a similar immunodeficiency syndrome with neurological sequelae. to identify the neurophysiological substrate of fiv infection in brain, pathogen-free cats were infected with the maryland strain of fiv. eight weeks after inoculation, all fiv-infected cats seroconverted and virus was detected in the cerebr ... | 1994 | 7809152 |
| autologous red cell agglutination test for antibodies to feline immunodeficiency virus. | the t-lymphotropic lentivirus, feline immunodeficiency virus (fiv) is now recognised as a major viral pathogen affecting domestic cat populations worldwide. a rapid, autologous red cell agglutination test for antibodies to fiv has been developed. a synthetic peptide analog corresponding to the immunodominant epitope within the fiv transmembrane glycoprotein gp40 residues (680-715) kveamekflytafamqelgc (acm)nqnqffk(brac)kiplelwtr was conjugated to an anti-feline erythrocyte antibody using a thio- ... | 1994 | 7810059 |
| protection against homologous but not heterologous challenge induced by inactivated feline immunodeficiency virus vaccines. | whole inactivated virus vaccines from the fl4 cell line protected against challenge with homologous feline immunodeficiency virus (petaluma strain) but not against a heterologous fiv isolate (gl-8) which is distinct from the petaluma strain in virus neutralization. protection was associated with a type-specific neutralizing antibody response and was retained when the challenge virus was propagated in an unrelated cell line. | 1995 | 7815500 |
| effect of 3'azido-2',3'-deoxythymidine (azt) on experimental feline immunodeficiency virus infection in domestic cats. | the compound 3'azido-2',3'-deoxythymidine (azt) inhibits the replication of feline immunodeficiency virus (fiv) in cell culture, and treatment with the compound has been reported to induce some clinical improvement in some cases of feline fiv infection. in order to determine the effect of prophylactic treatment with azt on experimental fiv infection, cats were treated with the compound at 0.2, 1.0, 5, 25 or 50 mg kg-1 day-1 for 29 days. one day after the treatment was started, they were inoculat ... | 1994 | 7817010 |
| intraventricular administration of a fiv-envelope protein induces sleep architecture changes in rats. | fifteen adult male sprague-dawley rats were implanted with a set of electrodes for standard sleep recordings. a stainless steel cannula was also implanted into the lateral ventricle of these rats. fifteen additional rats were implanted with a cannula alone. rats with electrodes were habituated for 3 days or more to the recording environment, then placed into 3 groups (n = 5). one group received saline (i.c.v.), while the other two groups received either the feline immunodeficiency virus envelope ... | 1994 | 7820670 |
| feline immunodeficiency virus infection of macrophages: in vitro and in vivo inhibition by dideoxycytidine-5'-triphosphate-loaded erythrocytes. | although hiv-1 and other mammalian lentiviruses infect macrophages, they are not cytopathic. consequently, these infected long-lived cells serve as major virus reservoirs with a key role in the propagation of the virus throughout the body as well as in the pathogenesis of aids. furthermore, well-differentiated macrophages possess low abilities to phosphorylate the most common reverse transcriptase inhibitors of the nucleoside analog family. in an attempt to overcome these problems we have evalua ... | 1994 | 7826702 |
| hemostatic disorders in feline immunodeficiency virus-seropositive cats. | the hemostatic function of 40 feline immunodeficiency virus (fiv) seropositive and 8 fiv and feline leukemia virus (felv) seropositive cats was evaluated and compared with reference values from 30 clinically healthy cats. the fiv-positive cats were divided into 3 groups: group i included asymptomatic carriers; group ii comprised sick fiv-infected cats with illnesses not likely to influence the hemostatic system; and group iii included fiv-positive cats with diseases potentially associated with c ... | 1994 | 7837113 |
| substantiation of immunosuppression. | 1994 | 7848448 | |
| marrow accessory cell infection and alterations in hematopoiesis accompany severe neutropenia during experimental acute infection with feline immunodeficiency virus. | severe neutropenia and bone marrow (bm) morphologic abnormalities occur during experimentally induced primary infection with feline immunodeficiency virus (fiv), a lentivirus biologically similar to human immunodeficiency virus (hiv). to further characterize the mechanisms involved in this acute infection model of lentivirus-induced bm suppression, peripheral blood counts, histologic bm studies, and bm culture assays were performed on 12 cats that underwent necropsy at regular intervals postinoc ... | 1995 | 7849316 |
| an experimental study of primary feline immunodeficiency virus infection in cats and a historical comparison to acute simian and human immunodeficiency virus diseases. | sixteen adolescent specific pathogen free cats were inoculated with the petaluma strain of feline immunodeficiency virus (fiv) and two cats were then necropsied at each of 5, 10, 21, 28, 42, 56, 70, and 84 day time points following infection. lymphadenopathy gradually increased starting at day 10 and persisted for the duration. gross clinical signs of fever, mild to severe malaise, anorexia, diarrhea, dehydration, and generalized soreness appeared around day 42, peaked at day 56, and disappeared ... | 1994 | 7856070 |
| differences in feline immunodeficiency virus host cell range correlate with envelope fusogenic properties. | feline immunodeficiency virus (fiv) establishes persistent infections in cats inducing an acquired immunodeficiency syndrome. differences in cell tropism have been observed among isolates of fiv (t. r. phillips et al., j. virol. 64, 4605-4613, 1990). the progeny of the infectious molecular clone of fiv p34tf10 was able to productively infect a feline fibroblast cell line, crandell feline kidney cell, (crfk), while the progeny of the molecular clone pppr was not. however, pppr, after transfection ... | 1995 | 7856093 |
| comparative functional analysis of the various lentivirus long terminal repeats in human colon carcinoma cell line (sw480 cells) and feline renal cell line (crfk cells). | basal promoter activities of various lentiviral long terminal repeats (ltrs) in a human colon carcinoma cell line (sw480 cells) and a feline renal cell line (crfk cells) were examined by the chloramphenicol acetyltransferase (cat) assay using the ltr-cat reporter plasmids. in sw480 cells, the basal promoter activities induced by ltrs of visna virus, caprine arthritis-encephalitis virus (caev), and simian immunodeficiency virus (sivagm) were moderate, and those induced by ltrs of human immunodefi ... | 1994 | 7865591 |
| mlui site-dependent transcriptional regulation of the candida albicans dutpase gene. | the candida albicans dutp pyrophosphatase (dutpase) gene dut1 has been isolated by genetic complementation in s. cerevisiae. it was found to encode a 17-kda protein similar in amino-acid sequence to dutpases isolated from other systems. the gene was adapted for expression in e. coli and yielded a soluble and highly-active enzyme which is easily purified. the 5' flanking sequence of dut1 contains an mlui site typical of mcb cell-cycle-dependent uas elements of budding and fission yeast. we found ... | 1994 | 7874734 |
| mammalian cell/vaccinia virus expression vectors with increased stability of retroviral sequences in escherichia coli: production of feline immunodeficiency virus envelope protein. | many eukaryotic dna sequences, especially lenti-retrovirus proviral genomes and their env genes, are unstable when cloned in high-copy-number plasmids in escherichia coli. stability can be increased by the use of low-copy-number vectors, although plasmid yields are low. vectors are described here that contain the intermediate-copy-number p15a ori for cloning, stable propagation and higher-yield production of plasmid dna in e. coli, and the f1 ori for propagation as single-stranded phage. these v ... | 1995 | 7875588 |
| structural and functional studies of retroviral rna pseudoknots involved in ribosomal frameshifting: nucleotides at the junction of the two stems are important for efficient ribosomal frameshifting. | ribosomal frameshifting, a translational mechanism used during retroviral replication, involves a directed change in reading frame at a specific site at a defined frequency. such programmed frameshifting at the mouse mammary tumor virus (mmtv) gag-pro shift site requires two mrna signals: a heptanucleotide shifty sequence and a pseudoknot structure positioned downstream. using in vitro translation assays and enzymatic and chemical probes for rna structure, we have defined features of the pseudok ... | 1995 | 7882986 |
| structural analysis of the principal immunodominant domain of the feline immunodeficiency virus transmembrane glycoprotein. | in the transmembrane envelope glycoprotein (tm) of lentiviruses, including human immunodeficiency virus type 1 (hiv-1) and feline immunodeficiency virus (fiv), two cysteine residues, conserved in most retroviruses, are thought to form a loop containing five to seven amino acids. these elements make up a b-cell epitope recognized by nearly 100% of sera from infected patients or animals, designated the principal immunodominant domain (pid). the pid amino acid sequences are highly conserved between ... | 1995 | 7884857 |
| chimeras of receptors for gibbon ape leukemia virus/feline leukemia virus b and amphotropic murine leukemia virus reveal different modes of receptor recognition by retrovirus. | glvr1 encodes the human receptor for gibbon ape leukemia virus (galv) and feline leukemia virus subgroup b (felv-b), while the related gene glvr2 encodes the human receptor for amphotropic murine leukemia viruses (a-mlvs). the two proteins are 62% identical in their amino acid sequences and are predicted to have 10 transmembrane domains and five extracellular loops. a stretch of nine amino acids (region a) in the predicted fourth extracellular loop was previously shown to be critical for the fun ... | 1995 | 7884886 |
| early stages of feline immunodeficiency virus infection in lymph nodes and spleen. | analysis of the early stages of infection within the lymphoid organs is crucial for the understanding of the physiopathology of hiv infection. such analysis can only be performed using animal models. cats were infected with two strains of fiv and killed at regular intervals for a classic pathologic study along with a quantification of the viral load by in situ hybridization in the spleen and the lymph nodes. the pathological study showed a persistent follicular reaction, which peaked 15 days pos ... | 1994 | 7888233 |
| superinfection of cats with feline immunodeficiency virus subtypes a and b. | the ability of feline immunodeficiency virus (fiv) isolates from subtypes a and b to superinfect cats and cell cultures was tested. three specific pathogen-free (spf) cats were first inoculated with 10 id50 of subtype b virus (fivbang) and 30 weeks later inoculated with 100 id50 of subtype a virus (fivpet). on the basis of subtype-specific pcr analysis, both fiv subtypes were detected in the peripheral blood lymphocytes (pbls) of two of three cats from 9 to 30 weeks following the second inoculat ... | 1994 | 7888234 |
| early haematological and pathological abnormalities of pathogen-free cats experimentally infected with feline immunodeficiency virus (fiv). | twelve 8-12-week-old specific-pathogen-free (spf) cats were inoculated intraperitoneally with feline immunodeficiency virus, or with blood from inoculated cats. three cats of the same age were used as controls. all animals were sacrificed 10 weeks after inoculation. the inoculated cats seroconverted between the 3rd and 6th weeks after inoculation. for 6 infected cats, a decrease in the cd4/cd8 lymphocyte ratio was observed as early as the 6th to the 10th week after inoculation. granular lymphocy ... | 1994 | 7889036 |
| novel, negatively charged, human serum albumins display potent and selective in vitro anti-human immunodeficiency virus type 1 activity. | we prepared a series of modified proteins and peptides by derivatizing the positively charged epsilon-amino groups of the lysine amino acids through reaction with anhydrides of succinic acid (suc) and aconitic acid (aco). human serum albumin (hsa) was modified by introduction of a single carboxylic group (suc-hsa) or two carboxylic groups (aco-hsa) per amine function, yielding strongly negatively charged compounds. the in vitro anti-human immunodeficiency virus (hiv)-1 ic50 of suc-hsa was about ... | 1993 | 7902528 |
| malignant lymphoma associated with experimentally induced feline immunodeficiency virus infection. | a malignant low-grade b-cell lymphoma, primarily in the kidney, is described in a specific-pathogen-free cat experimentally infected with feline immunodeficiency virus (fiv) and free of feline leukaemia virus. at the time of diagnosis the cat showed a marked reduction of circulating cd4+ t lymphocytes, was 2 years old, and had been infected for 18 months. fiv was isolated both from peripheral blood mononuclear cells and the neoplastic tissue. dna of fiv gag gene was detected in several specimens ... | 1994 | 7914523 |
| effects of incidental infections and immune activation on disease progression in experimentally feline immunodeficiency virus-infected cats. | specific pathogen-free cats were experimentally infected with feline immunodeficiency virus (fiv) and subsequently exposed to common infectious pathogens and immune stimuli over a 3-year period. cats with preexisting fiv infection showed signs of disease after exposure to haemobartonella felis, toxoplasma gondii, feline herpesvirus-1, and feline calicivirus similar to signs in non-fiv-infected cats, although they were more severe. no adverse effects of immunization with inactivated rabies virus ... | 1994 | 7916048 |
| inhibition of feline immunodeficiency virus gene expression and replication by alphaherpesvirus icp4 homologues. | we investigated the effects of pseudorabies virus (prv), herpes simplex virus type 1 (hsv-1), and equine herpesvirus type 1 (ehv-1) icp4 homologues on feline immunodeficiency virus (fiv) long terminal repeat (ltr)-directed gene expression. this was done by using the transient expression chloramphenicol acetyltransferase (cat) assay in crandell feline kidney (crfk) and felis catus whole fetus 4 cells transfected with a chimeric fiv ltr-cat reporter construct in combination with effector plasmids ... | 1994 | 7931167 |
| modulation of host cell growth during fiv infection in vitro. | 1994 | 7932088 | |
| analysis of the vif gene of feline immunodeficiency virus. | we have examined fiv vif function in the context of molecular clone pf34 (genbank accession no. m25729). rabbit antisera directed against the translation product of the vif gene identified a 29-kda protein in tissue culture cells infected with fiv-pf34; this protein was not present in cultures of uninfected cells. thus, the vif gene of this virus strain is expressed in infected cells. three mutations were made in distinct regions of the vif gene of molecular clone fiv-pf34: (i) deletion of 223 b ... | 1994 | 7941360 |
| evaluation of in vivo and in vitro interactions of feline immunodeficiency virus and feline leukemia virus. | to determine the potential mechanisms for disease potentiation where feline immunodeficiency virus (fiv) infection of persistently feline leukemia virus (felv)-infected cats results in more severe fiv disease and increased mortality than fiv infection of specific pathogen-free cats. | 1994 | 7946095 |
| histopathology and viral antigen distribution in lymph nodes of cats naturally infected with feline immunodeficiency virus. | lymph nodes from cats with natural feline immunodeficiency virus (fiv) infection were examined histopathologically for characterization of lesions in the symptomatic and terminal stages. the localization of viral antigen was also studied in the nodes by immunohistochemistry using a monoclonal antibody to fiv p24. in a node of persistent generalized lymphadenopathy stage, follicular hyperplasia was prominent and the viral antigen was demonstrated predominantly in the follicular lymphocytes and ti ... | 1994 | 7948383 |
| a neutralizing antibody-inducing peptide of the v3 domain of feline immunodeficiency virus envelope glycoprotein does not induce protective immunity. | specific-pathogen-free cats, immunized with a 22-amino-acid synthetic peptide designated v3.3 and derived from the third variable region of the envelope glycoprotein of the petaluma isolate of feline immunodeficiency virus (fiv), developed high antibody titers to the v3.3 peptide and to purified virus, as assayed by enzyme-linked immunoassays, as well as neutralizing antibodies, as assayed by the inhibition of syncytium formation in crandell feline kidney cells. v3.3-immunized animals and contro ... | 1994 | 7966629 |
| competitive polymerase chain reaction for quantitating feline immunodeficiency virus load in infected cat tissues. | to quantitate fiv provirus copy numbers present in tissue of infected cats, we have applied a competitive polymerase chain reaction (cpcr) recently described for hiv. the method consists in coamplifying a fixed amount of the dna to be examined with graded copy numbers of a dna competitor incorporating a short deletion and bearing the same primer recognition sequences. these conditions ensure almost identical thermodynamic and amplification efficiency for both template species but permit a prompt ... | 1994 | 7969197 |
| viral diseases as a threat to free-living wild cats (felis silvestris) in continental europe. | 1994 | 7975059 | |
| infection of cats with molecularly cloned and biological isolates of the feline immunodeficiency virus. | molecularly cloned viruses are considered essential reagents for characterizing viral domains responsible for infectivity and disease pathogenesis in the host. the infectivity and hematological alterations associated with two molecularly cloned isolates of feline immunodeficiency virus (fiv-pppr and fiv-pf34) and an uncloned isolate (fiv-ppr) were assessed by inoculation of cats. inoculated cats were tested for viral antibody, viremia, and clinical pathological disease. peripheral blood mononucl ... | 1994 | 7975256 |
| is it possible to catch leukaemia from a cat? | 1994 | 7983981 | |
| virus neutralization reveals antigenic variation among feline immunodeficiency virus isolates. | by using a focus reduction assay in crfk feline fibroblast cells, virus neutralizing antibodies (vna) to feline immunodeficiency virus (fiv) were demonstrated in cats that had been naturally or experimentally infected with fiv. the antigenic relatedness of four strains of fiv, divergent in nucleotide sequence within the env gene, was investigated by neutralization following adaptation of each virus for growth in crfk cells. two of the viruses were from the netherlands (fiv/am-4 and am-6), one wa ... | 1994 | 7996159 |
| feline immunodeficiency virus can productively infect cultured endothelial cells from cat brain microvessels. | feline immunodeficiency virus (fiv) provokes a disease in cats characterized by histopathological lesions similar to those observed in aids patients. in order to determine whether endothelial cells from brain microvessels are involved in the central nervous system disease to the same extent as macrophages and microglia, cells were isolated from healthy cat brains, cultured and infected in vitro with the fiv villefranche iffa 1/88 strain. the isolated cells displayed typical endothelial cell ultr ... | 1994 | 7996160 |
| the induction of in vivo superinfection and recombination using feline immunodeficiency virus as the model. | this study investigated the hypothesis that under certain conditions, superinfection of cats with feline immunodeficiency virus (fiv), may occur. one fiv isolate (t91) was used to inoculate three fiv and felv-free cats. blood from an fiv-infected cat (n), which contained two variants and differed from t91 by at least 5% in nucleotide sequence in the env gene, was inoculated into a fourth cat. both t91 and blood from n were inoculated simultaneously into a fifth cat. after 22 weeks, two of the th ... | 1994 | 7998833 |
| clinical aspects of chlamydia psittaci infection in cats infected with feline immunodeficiency virus. | two groups of six cats were established, one a control group and one infected with feline immunodeficiency virus (fiv) 18 months previously. the cats in both groups were inoculated with chlamydia psittaci and the clinical progression of the infection was monitored by means of a clinical scoring system for 10 months. haematological, serological and viral and chlamydial isolation studies were also made. the response of the fiv infected group to treatment with oxytetracycline was monitored in the 1 ... | 1994 | 8009798 |
| molecular properties of feline immunodeficiency virus (fiv). | 1993 | 8012737 | |
| tenth anniversary perspectives on aids. fiv as a model for aids vaccination. | 1994 | 8018382 | |
| the development of a vaccine against feline immunodeficiency virus. | feline immunodeficiency virus (fiv) is a retrovirus causing significant disease in cats. the virus has been shown to be similar biologically to the human immunodeficiency virus (hiv), the cause of acquired immunodeficiency syndrome (aids) in humans. much interest has been expressed in the use of fiv as an animal model for hiv vaccination studies. both fiv and hiv belong to the lentivirus group of retroviruses. while there are several effective vaccines against feline leukaemia virus (felv), a ma ... | 1994 | 8025833 |
| fiv and hiv vaccines--problems and prospects. | 1994 | 8025838 | |
| serologic survey and serum biochemical reference ranges of the free-ranging mountain lion (felis concolor) in california. | serum samples from 58 mountain lions (felis concolor) in california (usa) were collected between april 1987 and february 1990. nineteen serum samples were used for serum biochemistry determinations; the ranges were similar to reference values in domestic cats, captive exotic felidae and free-ranging mountain lions. a serological survey was conducted to determine whether antibodies were present against selected infectious agents. fifty-four (93%) of 58 sera had antibodies against feline panleukop ... | 1994 | 8028105 |
| nucleotide sequence analysis of puma lentivirus (plv-14): genomic organization and relationship to other lentiviruses. | the complete nucleotide sequence of an isolate of puma lentivirus (plv-14) was obtained by an inverse polymerase chain reaction (i-pcr) technique and confirmed by conventional pcr. both methods were used to amplify overlapping regions of proviral dna, for cloning and sequencing, from genomic dna isolated from plv-14 infected florida puma (felis concolor coryi) peripheral blood mononuclear cells (pbmc). the provirus has a total length of 9100 nucleotides and the genomic organization of presumed p ... | 1994 | 8030248 |
| neuropathologic aspects of lentiviral infections. | studies of lentiviral infections of various animals and man have shown that all may invade the cns and induce pathological lesions. this is well established in infections with vv, caev, siv, hiv-1, and fiv. although vv and caev do not cause an overt immunodeficiency, they share several features pertinent for the establishment of neuropathologic lesions with those that induce immunodeficiency. this holds especially true for the initial steps and early cns lesions. 1) infection of the cns is from ... | 1994 | 8030977 |
| multiple-drug-resistant mutants of feline immunodeficiency virus selected with 2',3'-dideoxyinosine alone and in combination with 3'-azido-3'-deoxythymidine. | mutants of feline immunodeficiency virus (fiv) were selected in cell culture in the continuous presence of 10 microm (each) 3'-azido-3'-deoxythymidine (azt) and 2',3'-dideoxyinosine (ddi). these mutants (air-1 and air-3) displayed a 13-fold resistance to azt but had less than a 2-fold decrease in susceptibility to ddi. interestingly, the air mutants were cross-resistant to phosphonoformate (pfa) and were hypersensitive to 2',3'-dideoxycytidine (ddc). mutants of fiv were also selected in the pres ... | 1994 | 8031060 |
| effect of chronic feline immunodeficiency virus infection on experimental feline calicivirus-induced disease. | acute feline calicivirus (fcv) infection caused a more severe disease in chronically feline immunodeficiency virus (fiv) infected than in non-fiv infected cats. fiv infected cats shed significantly higher amounts of fcv through their saliva after fcv challenge than the non-fiv infected cats. however, there was no difference in the duration of fcv shedding. none of the cats exposed to fcv developed chronic fcv carriage. both groups of cats mounted similar titers of neutralizing antibodies to fcv. ... | 1994 | 8042279 |
| seroepidemiological and clinical survey of feline immunodeficiency virus infection in northern italy. | four hundred and thirty-nine feline serum samples from cats with different living conditions in the north of italy were tested for antibodies to feline immunodeficiency virus (fiv) and for antigen of feline leukemia virus by enzyme-linked immunosorbent assay. a western blot technique was also used on the positive sera in order to confirm the presence of specific antibodies to fiv. the western blot enabled the detection of a false positive serum. the prevalence of fiv infection in this population ... | 1994 | 8042281 |
| comparison of biological properties of feline immunodeficiency virus isolates using recombinant chimeric viruses. | the biological properties of homogeneous populations of feline immunodeficiency viruses derived from infectious, molecular clones of the tm1, tm2 and petaluma strains were compared. differences in infectivity for crandell feline kidney (crfk) cells, and in syncytium formation and replication kinetics in a feline t lymphoblastoid cell line (mya-1 cells) were observed. to investigate the basis of these differences between the tm2 and petaluma strains, we first compared the basal promoter activity ... | 1994 | 8046395 |
| removal of the cleavage site of recombinant feline immunodeficiency virus envelope protein facilitates incorporation of the surface glycoprotein in immune-stimulating complexes. | recombinant vaccinia viruses were constructed that expressed the complete env gene of feline immunodeficiency virus with or without the nucleotide sequence encoding the cleavage site between the surface (su) protein and the transmembrane (tm) protein. the removal of this cleavage site resulted in the expression of a 150k protein that was processed into a 130k protein and was not cleaved into the su and the tm proteins. removal of the cleavage site also facilitated incorporation of the su protein ... | 1994 | 8046415 |
| feline spinal lymphosarcoma: a retrospective evaluation of 23 cats. | a retrospective study of pathologically confirmed cases of feline spinal lymphosarcoma (fsl) admitted to the colleges of veterinary medicine at the university of georgia and north carolina state university from 1973 to 1988 was conducted. two hundred fourteen cases of feline lymphosarcoma were diagnosed histopathologically; involvement of the central nervous system (cns) was identified in 26 (12.1%). twenty-three of these tumors involved the spinal cord, and 22 of the 23 were solitary. a predile ... | 1994 | 8046683 |
| [diagnosis of fiv infection]. | the fiv petcheck elisa is a practical and commercially available method routinely used for the detection of anti-fiv antibodies in fiv infection. however, false positive and false negative results occur, although the reliability of the negative results is higher than that of the positive results. to determine the predictive value of a positive result in bavaria, 1911 randomly selected cat sera were collected. using the petcheck anti-fiv elisa, 103 (5.4%) of the 1911 samples tested were positive. ... | 1994 | 8048037 |
| [epidemiology of felv and fiv infection in the federal republic of germany]. | in a nationwide study 6101 cats were tested for presence of feline leukemia virus (felv) antigen and for feline immunodeficiency virus (fiv) antibodies utilizing enzyme linked immunosorbent assay (elisa). 21.8% of the german cat population may be felv- and/or fiv-positive. of the animals tested, 13.4% were found to be felv carriers, while 8.4% showed evidence of fiv infection. infection with both viruses was identified in 2.1% of the cats tested. of the animals showing clinical symptoms, nearly ... | 1994 | 8048038 |
| photopheresis. | 1994 | 8050928 | |
| induction of feline immunodeficiency virus-specific cytotoxic t cells in vivo with carrier-free synthetic peptide. | the role of cellular immunity in the establishment and progression of immunosuppressive lentivirus infection remains equivocal. to develop a model system with which these aspects of the host immune response can be studied experimentally, we examined the response of cats to a hybrid peptide containing predicted t-and b-cell epitopes from the gag and env genes of feline immunodeficiency virus (fiv). cats were immunized with an unmodified 17-residue peptide incorporating residues 196 to 208 (from g ... | 1994 | 8057464 |
| a lion lentivirus related to feline immunodeficiency virus: epidemiologic and phylogenetic aspects. | feline immunodeficiency virus (fiv) is a novel lentivirus that is genetically homologous and functionally analogous to the human aids viruses, human immunodeficiency virus types 1 and 2. fiv causes immunosuppression in domestic cats by destroying the cd4 t-lymphocyte subsets in infected hosts. a serological survey of over 400 free-ranging african and asian lions (panthera leo) for antibodies to fiv revealed endemic lentivirus prevalence with an incidence of seropositivity as high as 90%. a lion ... | 1994 | 8057472 |
| epidemiologic observations on infection with rochalimaea species among cats living in baltimore, md. | cats from several sources in baltimore, md, were tested for seropositivity to rochalimaea henselae and r quintana. co-infection with toxoplasma gondii or feline immunodeficiency virus was assessed as a risk factor for infection with rochalimaea spp. of 592 cats tested, 87 (14.7%) were seropositive for one or both rochalimaea spp, although titers to r henselae were significantly higher than those to r quintana. prevalence of seropositivity increased significantly with cat age and weight and was a ... | 1994 | 8063598 |
| pathological findings in a cat with cryptococcosis and feline immunodeficiency virus infection. | this report describes the gross, histopathological, immunocytochemical and electron microscopic findings in a cat with systemic cryptococcosis and feline immunodeficiency virus (fiv) infection. lymphadenopathy and cloudiness of leptomeninges were the major gross findings. numerous cryptococcal yeasts were found in lymph nodes, brain, and lung, and were less common in the kidney and the eye. the inflammatory reaction varied in intensity and cell type (mononuclear through granulomatous) depending ... | 1994 | 8075490 |
| development of clinical disease in cats experimentally infected with feline immunodeficiency virus. | cats naturally infected with feline immunodeficiency virus (fiv) develop an aids-like syndrome whereas experimentally infected cats do not. to investigate the role of cofactors in the development of this disease in cats, 7 specific pathogen-free (spf) and 12 random-source (rs) cats were infected with fiv. over 4 years, infected cats developed similar phenotypic and functional immune abnormalities characterized by early and chronic inversion of cd4+:cd8+ cell ratios and significantly decreased mi ... | 1994 | 8077711 |
| feline immunodeficiency virus decreases cell-cell communication and mitochondrial membrane potential. | the in vitro effects of viral replication on mitochondrial membrane potential (mmp) and gap junctional intercellular communication (gjic) were evaluated as two parameters of potential cellular injury. two distinct cell types were infected with the petaluma strain of feline immunodeficiency virus (fiv). primary astroglia supported acute fiv infection, resulting in syncytia within 3 days of infection, whereas immortalized crandell feline kidney (crfk) cells of epithelial origin supported persisten ... | 1994 | 8084006 |